辅酶Q_(10)对腹水症肉鸡脂质代谢以及抗氧化能力的影响

研究日粮中添加辅酶Q10对腹水症肉鸡脂质代谢以及抗氧化能力的影响。将180只AA雄性肉仔鸡随机分为3组,每组6个重复,8日龄起在日粮中添加不同水平的辅酶Q10,分别为0、20、40mg/kg。15-21日龄关闭水暖进行较低温处理(15-18℃)以诱发腹水症。结果表明:日粮中添加40 mg/kg辅酶Q10显著降低腹水症肉鸡机体内TG、TC和LDL-C含量(P<0.05),而HDL-C没有变化;显著提高TAOC和降低MDA含量(P<0.05)。这表明辅酶Q10相对长期添加时能够改善肉鸡脂质代谢以及总抗氧化能力。     

辅酶Q10对腹水症敏感肉鸡肝线粒体功能及抗氧化能力的影响

本文旨在研究添加辅酶Q10对腹水症敏感肉鸡肝脏线粒体功能及抗氧化能力的影响。选用1日龄艾维茵肉公鸡160只,随机分为4组,分别为大肠杆菌内毒素注射(lipopolysacchride,LPS)组(1.0 mg/kg体重剂量,腹腔注射)、LPS注射 40 mg/kg辅酶Q10添加组、生理盐水注射组、生理盐水 40 mg/kg辅酶Q10添加组,每组40只,设5个重复,每个重复8只。肉鸡饲以全植物性玉米-豆粕基础日粮,试验期6周。辅酶Q10从1日龄开始添加,10日龄开始突然关闭水暖,进行低温处理(12~15℃)以提高肉鸡腹水症敏感性。试验结果显示:(1)添加辅酶Q10肉鸡红细胞压积(PCV)、腹水心脏指数(AHI)显著降低(P<0.05)。(2)添加辅酶Q10肉鸡肝脏线粒体状态3、状态4呼吸速率、呼吸控制率(RCR)、磷氧比(P/O)变化虽不显著(P>0.05),但线粒体呼吸链复合体Ⅱ Ⅲ的质子转移与电子传递关系(H /2e)比值显著提高(P<0.05)。(3)添加辅酶Q10细胞色素C氧化酶(CCO)和H -ATP酶活性提高(P<0.05),但对线粒体NADH-细胞色素C还原酶(NCCR)和琥珀酸-细胞色素C还原酶(SCCR)活性都没有显著影响(P>0.05)。(4)添加辅酶Q10时,线粒体抗活性氧能力(ARC)显著提高(P<0.05),脂质过氧化产物丙二醛(MDA)含量显著降低(P<0.05),血清中总超氧化物歧化酶(T-SOD)活性显著升高(P<0.05),而线粒体内T-SOD、血清中过氧化氢酶(CAT)、抗超氧阴离子能力(ASAC)、ARC没有显著变化(P>0.05)。本试验结果表明,添加辅酶Q10能够在某种程度上改善腹水症敏感肉鸡的肝脏线粒体功能以及一些抗氧化能力。     

辅酶Q10的制备及应用新进展

辅酶Q10是一种广泛存在于生物体中的重要生理活性物质,具有提高人体免疫力、增强抗氧化能力、延缓衰老等功能.目前,生物合成法是工业生产制备辅酶Q10的主要方式和未来趋势,辅酶Q10的应用正从医药领域逐渐拓展到食品、保健品和化妆品等更宽广的领域.对辅酶Q10的制备方法及应用新进展进行了概述.     

辅酶Q10生物学功能及其在肉鸡腹水中的应用进展

CoQ_(10)是呼吸链的重要成分,参与细胞氧化磷酸化及ATP生成过程,是细胞的天然抗氧化剂,细胞代谢的激活剂,能够提高机体的免疫力。本文介绍了CoQ_(10)的研究和应用情况、生物学功能及在肉鸡腹水中的研究进展,建议积极探索CoQ_(10)在其他动物疾病中的应用,提高对动物疾病的预防和保健能力。     

辅酶Q10制备技术及其在医学中的应用

辅酶Q10是细胞线粒体呼吸链重要成员之一,是细胞自身产生的天然抗氧化剂和细胞代谢的激活剂。文章综述了辅酶Q10的理化性质、制备技术、在心血管病临床和其他医学方面的应用研究。     

辅酶Q10对湖羊精液常温保存效果的影响

本实验旨在探究辅酶Q10对湖羊精液常温保存效果的影响.选取3只1～2岁的健康湖羊采集精液,在基础稀释液中添加0、5、50、250、500μmol/L的辅酶Q10,检测在20℃保存过程中的湖羊精子活力、活率、曲线速度、质膜完整率、顶体完整率和活性氧(ROS)含量等指标.结果表明:随着在常温保存稀释液中添加辅酶Q10浓度的...     

辅酶Q10对肉仔鸡生产性能的影响以及对腹水症的敏感性研究

180只AA雄性肉仔鸡随机分为3组,每组6个重复,8日龄起分别饲喂代谢能为13.2MJ/kg的日粮,辅酶Q10添加量分别为0、20、40mg/kg.15～21日龄关闭水暖进行较低温处理(15～18℃)以诱发腹水症,并研究辅酶Q10添加对肉仔鸡生产性能的影响以及对腹水症的敏感性.结果表明在本试验条件下,肉仔鸡日粮中添加不同水平的辅酶Q10对生产性能的影响不显著,而能显著降低其对腹水症的敏感性.日粮添加辅酶Q10能够显著降低肉仔鸡腹水症的发病率和死亡率,降低肉仔鸡红细胞渗透脆性(EOF)(P≤0.05).虽然辅酶Q10添加与否对血液红细胞压积(PCV)没有显著影响,但40mg/kg的辅酶Q10添加量同20mg/kg的添加量相比,PCV值显著降低(P≤0.05),除了36日龄时添加40mg/kg辅酶Q10显著降低了肉仔鸡的肺动脉舒张压(PADP)外,各试验组肉仔鸡右心室内压(RVP)、肺动脉压(PAP)、右心室内压最大变化速率(±dp/dtmax)等均没有表现出显著变化,而40mg/kg的辅酶Q10组显著降低了腹水心脏指数(AHI)(P≤0.05).辅酶Q10的这种有益作用可能与其为心肌细胞充足供能、降低血液的红细胞渗透脆性有关.     

辅酶Q10对猪精液17℃保存效果的影响

为提高猪精液常温保存效果,本试验探讨17℃保存过程中,在Modena稀释液在不同浓度辅酶Q10(Co-Q10)(0、10、15、25、35μg/mL)对猪精液质量的影响。在17℃条件下每隔24 h检测不同组的精子活力、pH,每隔48 h检测精液的渗透压、精子线粒体活性和质膜完整率。结果表明：保存第5天,25μg/mL Co-Q10组精子活力、线粒体活性、质膜完整率均高于其他组（P<0.05）,pH下降速度也最慢,并且对渗透压变化影响不大。综合考虑Co-Q10在猪精液17℃保存中的适宜添加浓度为25μg/mL。     

日粮添加辅酶Q10对芦花鸡蛋蛋黄中辅酶Q10含量、产蛋量和蛋品质的影响

为了研究在日粮中添加不同水平辅酶Q₁₀对芦花鸡蛋蛋黄中辅酶Q₁₀含量、产蛋量及蛋品质的影响,试验选取38周龄、健康的芦花蛋鸡180只,随机分为4组,每组45只,对照组饲喂基础日粮,试验组分别饲喂添加0.2%、0.4%、0.8%辅酶Q₁₀的基础日粮,在正式试验开始后的第20天、第30天从每组中随机选取35枚鸡蛋,15枚用于检测蛋品质,另外20枚用于检测蛋黄中辅酶Q₁₀的含量。结果表明：第20天和第30天时,3个试验组蛋黄中辅酶Q₁₀含量均显著高于对照组(P<0.05),在第30天时,0.8%辅酶Q₁₀组辅酶Q₁₀含量最高,达9.140μg/g;整个试验期间,各组产蛋量和主要蛋品质指标(蛋黄颜色、蛋黄比率、哈氏单位、蛋清比率、蛋壳重)无显著性差异(P>0.05)。说明在日粮中添加0.2%及以上的辅酶Q₁₀能够显著增加蛋黄中辅酶Q₁₀含量,对产蛋量及蛋品质指标无明显不良影响。     

辅酶Q_(10)抗氧化作用的研究进展

辅酶Q_(10),是生物体内广泛存在的脂溶性醌类化合物,参与生物氧化呼吸链中的电子传递,促进产生ATP,维持机体代谢与能量供应。同时,它也是重要的抗氧化剂和非特异性免疫增强剂。本文主要从自由基、动脉粥样硬化、协同物质三方面探讨辅酶Q_(10)的抗氧化作用及其抗氧化机理,为辅酶Q_(10)在畜禽生产中的应用提供一定参考。     

The effect of dietary coenzyme Q10 on plasma metabolites and hepatic gene expression in broiler breeder hens

ABSTRACT

1. This study was performed to evaluate the effects of dietary supplementation of coenzyme Q10 (CoQ10) on laying rate, body weight, plasma metabolites and some liver gene expression in broiler breeder hens.

2. A total of 128 broiler breeder hens (Arbor Acres Plus, 47 weeks of age) were randomly distributed to four dietary groups supplemented with different levels of CoQ10 (0, 300, 600 or 900 mg/kg diet) with four replicates of eight hens each. During 47–54 weeks of age, laying rate, egg mass and body weight were recorded weekly. To assay plasma biochemical indicators, blood samples were collected at 54 weeks of age. At the end of the experiment, for evaluating the abdominal fat weight, liver weight and expression of the adiponectin and proliferator-activated receptor-α (PPAR-α) genes in the liver, eight hens per treatment were selected, weighed and humanely killed by decapitation.

3. Dietary supplementation of CoQ10 linearly decreased abdominal fat weight, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities by increased levels of CoQ10. The plasma levels of glucose, cholesterol and alkaline phosphatase (ALP) activity were quadratically decreased by increased levels of CoQ10. The best plasma levels of glucose, cholesterol and ALP activity were estimated at 562.5, 633.3 and 517.8 mg CoQ10/kg diet, respectively. Adiponectin and PPARα gene expression exhibited a linear increased by increased levels of CoQ10.

4. In conclusion, addition of CoQ10 to the diet influenced lipid metabolism and expression of the adiponectin and PPAR-α genes, which might be partially due to the improvement in mitochondrial metabolism and energy production. However, further studies are necessary to determine the effects of CoQ10 on these indicators in broiler breeder hens during ageing.     

Effect of supplementation with the reduced form of coenzyme Q10 on semen quality and antioxidant status in dogs with poor semen quality: Three case studies

Oxidative stress owing to an imbalance between reactive oxygen species and antioxidants, such as coenzyme Q10 (CoQ10), is a major contributor to male infertility. We investigated the effects of the reduced form of CoQ10 (ubiquinol) supplementation on semen quality in dogs with poor semen quality. Three dogs received 100 mg of ubiquinol orally once daily for 12 weeks. Semen quality, serum testosterone, and seminal plasma superoxide dismutase (SOD) activity were examined at 2-week intervals from 2 weeks before ubiquinol supplementation to 4 weeks after the treatment. Ubiquinol improved sperm motility, reduced morphologically abnormal sperm, and increased seminal plasma SOD activity; however, it had no effect on testosterone level, semen volume, and sperm number. Ubiquinol supplementation could be used as a non-endocrine therapy for infertile dogs.     

Plasma Coenzyme Q10 and Tocopherols in Thoroughbred Race Horses: Effect of Coenzyme Q10 Supplementation and Exercise

The fundamental role of coenzyme Q10 (CoQ10) in cellular bioenergetics is well established in humans and several other species, yet there is no information about its role in horses. In continuation of our study of serum CoQ10 in Thoroughbred race horses, we examined the effect of CoQ10 supplementation and exercise on changes in plasma CoQ10 and α- and γ-tocopherol concentrations. Two groups of horses, five per group, were used. One group received 1.9 g of CoQ10 daily and the second group 3.4 g as a dietary supplement. Both groups were subjected to graduated training sessions that involved low-intensity exercise (gallop) followed by high-intensity exercise (breeze). CoQ10 supplementation resulted in a significant increase in plasma CoQ10 during low-intensity exercise, whereas the increase following high-intensity exercise was markedly attenuated in both groups (P < .05). There were no changes in plasma α- and γ-tocopherol concentrations following the two exercise sessions. γ-Tocopherol constituted approximately 5% of α-tocopherol; this is the first report of plasma γ-tocopherol in horses. The attenuated response of plasma CoQ10 in horses receiving CoQ10 supplementation following high-intensity exercise may be attributed to its consumption during severe oxidative stress. This finding indicates that in horses not receiving any CoQ10 supplementation, high-intensity exercise would result in a marked depletion of endogenous CoQ10 in plasma, which could compromise their health and physical performance. This documents the importance of CoQ10 in horses. The study also establishes the safety and tolerability of high-dose CoQ10 in Thoroughbred race horses.     

Coenzyme Q10 in Equine Serum: Response to Supplementation

Although the importance of coenzyme Q10 (CoQ10) in health and disease and its critical role in cellular bioenergetics have been well established in various species, there is a total lack of information on the role of CoQ10 in horses. Therefore, the present study was undertaken first to ascertain the occurrence of CoQ10 and/or possibly other homologs of coenzyme Q (CoQ) in horse serum, and then to examine the response to CoQ10 supplementation on serum CoQ10 concentration. The study was carried out with 2-year-old Thoroughbred horses. Total CoQ (reduced and oxidized forms) in serum was assayed by high-performance liquid chromatography. The data show that horse serum contains CoQ as CoQ10, at a much lower concentration as compared with that of humans and several other species. There is no evidence for the presence of coenzyme Q9 or other homologs of CoQ. On supplementation with CoQ10 at 800 mg a day (1.47 mg/kg body weight), there was a significant increase in serum CoQ10 concentration, approximately 2.7-fold at 60 days. CoQ10 supplementation at this dosage was found to be safe and well tolerated. Additional studies are needed to examine whether maintaining higher serum CoQ10 concentrations is of potential health benefit to the horses, as assessed by selected biochemical markers and also in terms of performance.     

Supplementation with exogenous coenzyme Q10 to media for in vitro maturation and embryo culture fails to promote the developmental competence of porcine embryos

The coenzyme Q10 (CoQ10) is a potent antioxidant with critical protection role against cell oxidative stress, caused by the mitochondrial dysfunction. This study evaluated the effects of CoQ10 supplementation to in vitro maturation (IVM) or embryo culture media on the maturation, fertilization and subsequent embryonic development of pig oocytes and embryos. Maturation (Experiment 1) or embryo culture (Experiment 2) media were supplemented with 0 (control), 10, 25, 50 and 100 μM CoQ10. The addition of 10–50 μM CoQ10 to the IVM medium did not affect the percentage of MII oocytes nor the fertilization or the parameters of subsequent embryonic development. Exogenous CoQ10 in the culture medium neither did affect the development to the 2–4‐cell stage nor rates of blastocyst formation. Moreover, the highest concentration of CoQ10 (100 μM) in the maturation medium negatively affected blastocyst rates. In conclusion, exogenous CoQ10 supplementation of maturation or embryo culture media failed to improve the outcomes of our in vitro embryo production system and its use as an exogenous antioxidant should not be encouraged.     

Combined effects of guanidinoacetic acid,coenzyme Q10 and taurine on growth performance,gene expression and ascites mortality in broiler chickens

High levels of guanidinoacetate acid (GAA) deteriorate growth response in broiler chickens. We propose using coenzyme Q₁₀, an antioxidant, and taurine (TAU), a methyl donor, to cope with the situation when high level of GAA included in diet. GAA was supplemented at 0 (control), 0.75, 1.5 and 2.25 g/kg in isoenergetic and isonitrogenous diets and fed to broilers (Cobb 500) from 1 to 40 days post‐hatch. Three additional diets were prepared by adding CoQ₁₀ (40 mg/kg), TAU (40 mg/kg) or their combination (both CoQ₁₀ and TAU at 40 mg/kg) to the 2.25 g/kg GAA group. The experimental design used was a completely randomized design. While weight gain (p = 0.038) and feed conversion ratio (p = 0.024) improved when GAA added at 1.5 g/kg, higher supplementation (2.25 g/kg) deteriorated these responses. These responses, however, were significantly restored by using CoQ₁₀, TAU or their combination. Abdominal fat deposition was significantly decreased when TAU added to broiler diets by virtue of upregulating peroxisome proliferator‐activated receptor alpha. Supplementing broiler diets with CoQ₁₀ and TAU or their combination significantly decrease ascites mortality. In conclusion, CoQ₁₀ and TAU have shown beneficial effects when high level of GAA included in broiler diets.     

维拉帕米对高盐诱发肉鸡腹水综合征的预防作用

将150只8日龄商品代AA雄性肉仔鸡随机分为对照组、高盐组（饮水中添加3．0g／L氯化钠）和高盐维拉帕米组（饮水中添加3．0g／L氯化钠及0．1g／L雏拉帕米）。分别于15、22、29、36、43、50日龄测定各组鸡肺动脉压（PAP）、红细胞比客（PCV）和腹水心脏指数（AHI），并统计腹水发生率。结果显示，50日龄时，高盐组腹水发生率极显著高于对照组和高盐维拉帕米组；15日龄时，高盐组和高盐维拉帕米组PAP极显著高于对照组，36、43、50日龄时，高盐组PAP显著或极显著高于对照组和高盐维拉帕米组；整个试验期间，高盐组PCV显著或极显著高于同日龄对照组和高盐维拉帕米组；29至50日龄，高盐组AHI显著或极显著高于同日龄对照组和高盐维拉帕米组。表明，雏拉帕米对高盐诱发的肺动脉高压、右心肥大和PCV的升高有抑制作用，能够降低腹水发生率，有效预防高盐诱发的肉鸡腹水综合征。     

Effects of epigallocatechin gallate on lipid metabolism and its underlying molecular mechanism in broiler chickens

The objective of this study was to investigate the effects of epigallocatechin gallate (EGCG) on fat metabolism and to establish the molecular mechanism of these effects in broilers. Seventy‐two 28‐day‐old male Ross 308 broiler chickens were divided into three groups with different levels of EGCG supplementation for 4 weeks: normal control (NC) group, L‐EGCG (a low‐level supplement of EGCG, 40 mg/kg body weight daily) and H‐EGCG (a high‐level supplement of EGCG, 80 mg/kg body weight daily). After 4 weeks of oral administration, EGCG significantly reduced the level of abdominal fat deposition in broilers. The serum triglycerides and low‐density lipoprotein cholesterol of chickens in H‐EGCG group were also significantly decreased compared with the NC group, and the high‐density lipoprotein cholesterol was notably increased at the same time. Moreover, the vital role of the liver and abdominal adipose tissue in lipid metabolism of poultry animals was examined through gene expression and enzyme activities related to fat anabolism and catabolism in these organs. Our data show that EGCG supplementation for 2 weeks significantly downregulated the expression of fatty acid synthesis and fat deposition‐related genes, and upregulated the expression of genes involved in fatty acid β‐oxidation and lipolysis genes. Simultaneously, the activities of hepatic fatty acid synthesis enzymes (fatty acid synthase and acetyl CoA carboxylase) were significantly decreased, and the activity of carnitine palmitoyl transferase‐1 was notably elevated. The results suggest that EGCG could alleviate fat deposition in broilers through inhibiting fat anabolism and stimulating lipid catabolism in broilers.     

Protective effect of ubiquinone (coenzyme Q9) on ascites in broiler chickens

1. The effect of ubiquinone (C0Q9) on ascites in broiler chickens was investigated.

2. The commercial broilers were divided into 2 groups of 100 birds each; CoQ9‐treated group and non‐treated group.

3. The chickens were grown in a positive‐pressured house with double high efficiency particulate air filtered intakes and exhaust, and thus were strictly isolated from infectious agents.

4. The chickens (15 to 21 d old) were exposed to cold stress in order to induce ascites.

5. The number of birds with ascites in the C0Q3‐treated group was significantly lower than in the non‐treated group.

6. Survival and production rates were better in the CoQ^‐treated group than in the non‐treated group.     

日粮能量水平对皖南三黄鸡的生长和腹水综合征发生的影响

选取210只皖南三黄鸡,根据该系列商品代鸡的生产指标设计了低、中、高3种能量水平的日粮配方,能量值分别为12.81 MJ/kg、13.1 MJ/kg和13.38 MJ/kg,在常温下进行饲喂,每日称重,观察鸡群健康状况,试验持续6周。研究结果表明:高能量组生长性能都显著高于低、中能量组,尤其4周末和6周末时,高能量组平均体重显著高于其他2组。但是,高能量组腹水综合征的发病率和死亡率显著高于中、低能量水平组,分别为85.29%和6.90%。取腹水、肝脏、心脏等病料,显微镜检查未发现细菌,细菌培养呈阴性。有腹水症状鸡的红细胞压积(PCV)、血红蛋白(Hb)、红细胞计数(RBC)、乳酸脱氢酶(LDH)、谷丙转氨酶(GPT)及心脏指数(AHI)等指标均显著增高。以上结果显示:在肉鸡生产过程中,应协调好生长和腹水综合征发生之间关系,从而有利于发展皖南三黄鸡生产和减少腹水综合征所造成的经济损失。