Effect of water management on soil respiration and <Emphasis Type="Italic">NEE</Emphasis> of paddy fields in Southeast China

Water management is an important factor in regulating soil respiration and the net ecosystem exchange of CO₂ (NEE) between croplands and atmosphere. However, how water management affects soil respiration and the NEE of paddy fields remains unexplored. Thus, a 2-year field experiment was carried out to study the effects of controlled irrigation (CI) during the rice season on the variation of soil respiration and NEE, with flooding irrigation (FI) as the control. A decrease of irrigation water input by 46.39% did not significantly affect rice yield but significantly increased irrigation water use efficiency by 0.99 kg m^?3. The soil respiration rate of CI paddy fields was larger than that of FI paddy fields except during the ripening stage. Natural drying management during the ripening stage resulted in a significant increase of the soil respiration rate of the FI paddy fields. Variations of NEE with different water managements were opposite to soil respiration rates during the whole rice growth stages. Total CO₂ emission of CI paddy fields through soil respiration (total R _soil) increased by 11.66% compared with FI paddy fields. The increase of total R _soil resulted in the significant decrease of total net CO₂ absorption of CI paddy fields by 11.57% compared with FI paddy fields (p < 0.05). There were inter-annual differences of soil respiration and the NEE of paddy fields. Frequent alternate wetting and drying processes in the CI paddy fields were the main factors influencing soil respiration and NEE. CI management slightly enhanced the rice dry matter amount but accelerated the consumption and decomposition of soil organic carbon and significantly increased soil respiration, which led to the decrease of net CO₂ absorption. CI management and organic carbon input technologies should be combined in applications to achieve sustainable use of water and soil resources in paddy fields.     

Impacts on soil quality from long-term irrigation with treated greywater

Drought condition in many places leads to the imperative use of greywater for irrigation. There is a serious concern on the impact of such prolonged uses on soil sustainability. The objective of this study was to evaluate the long-term impacts of greywater irrigation on soil electrical conductivity (EC) and other soil quality parameters in field conditions. Six locations were monitored in this study where home gardens have been irrigated with treated greywater for 2 years. Results showed a general reduction in EC levels of soil samples along all depth intervals at all locations. The average soil EC before greywater irrigation was 0.97 dS/m and decreased to 0.41 dS/m, which may be due to the use of greywater as well as the rainwater effect. The reduction in soil EC and irrigation water quantity shows positive correlation (correlation coefficient r = 0.64). Calcium precipitation might also have a major role in soil EC reduction. Soil calcium content was 81 mg L^?1 before using treated greywater and decreased to 43 mg L^?1 after 2 years of treated greywater usage, which might have been caused by calcium carbonates (CaCO₃) precipitation. The results of other soil chemical analyses clearly show that using treated greywater for irrigation has reduced the concentration of organic matters, K, Cd, Pb, P, Mg, Cl, Na, exchangeable sodium percentage, and sodium adsorption ratio after 2 years of application. Zn concentration increased from 11 to 15 mg L^?1, and soil pH became slightly higher from 7.6 to 7.8.     

Modelling of groundwater recharge potential from irrigated paddy field under changing climate

Groundwater recharge from irrigated paddy field under various projected climate change scenarios was assessed using HYDRUS-1D model. Recharge flux, root water uptake, evaporation and surface runoff were simulated on daily time step for the growing period of paddy. Crop evapotranspiration and effective rainfall during the simulation period were estimated to be 301.9 and 269.4 mm, respectively. Cumulative bottom flux, root water uptake, evaporation and surface runoff were 69.2, 23.2, 30.8 and 0.0 cm for sandy loam and 37.2, 23.0, 30.8 and 0.7 cm for clay loam soils, respectively. Simulation results showed that the groundwater recharge potentials in sandy loam and clay loam soils with paddy crop are 69.2 and 37.2 cm, respectively. Cumulative recharge under various climate change scenarios from paddy field varied from 63.9 to 74.4 cm, 33.7 to 39.8 cm, 29.3 to 35.4 cm and 27.1 to 34.3 cm from land units A1 (sandy loam), B1 (clay loam with slight salinity), C1 (clay loam with moderate saline and slight sodic) and D1 (clay loam with strong saline and sodic), respectively. Cumulative recharge flux under the scenarios in which increase in relative humidity along with decrease in duration of sunshine hours was associated with rise in average temperature and wind speed, groundwater recharge would increase by 7.4 %. Cumulative recharge flux under the scenarios which were based on rise in temperature along with the increase in rainfall, groundwater recharge would increase by 0.2–3.9 %. Simulation results also showed that cumulative recharge would decrease under all those scenarios, which were based on rise in temperature only.     

Water and air temperature impacts on rice (<Emphasis Type="Italic">Oryza sativa</Emphasis>) phenology

Air temperature (T_a) is commonly used for modeling rice phenology. However, since the growing point of rice is under water during the vegetative and the early part of the reproductive period, water temperature (T_w) is likely to have a greater influence on crop developmental rates than T_a during this period. To test this hypothesis, we monitored T_w, T_a, and crop phenology in three commercial irrigated rice fields in California, USA. Sampling locations were set up on along a transect from the water inlet into the field. (Water warms up as it moves into the field.) T_a averaged 22.7 °C across sampling locations within each field, but average seasonal T_w increased from 22 °C near the inlet to 23.4 °C furthest away from the inlet. Relative to T_w furthest from the inlet, low T_w near the inlet delayed time to panicle initiation (PI 5 days) and heading (HD 8 days) and the appearance of one yellow hull on the main stem panicle (R7 9 days). Using T_w instead of T_a when the active growing point is under water until booting (midway between PI and HD) in a thermal time model improved accuracy (root-mean-square error, RMSE) for predicting time to PI by 2.5 days and HD by 1.6 days and R7 by 1.8 days. This model was further validated under more typical field conditions (i.e., not close to cold water inlets) in six locations in California. Under these conditions, average T_w was 2.6 °C higher than T_a between planting and booting, primarily due to higher daily maximum T_w values. Using T_w in the model until booting improved RMSE by 1.2 days in predicting time to HD. Using T_w instead of T_a during this period could improve the accuracy of rice phenology models.     

Effect of soil salinity on the wheat and bean root respiration rate at low matric suctions

Water logging and salinity often occur together because rising water table brings salt to the surface. We studied the effects of a range of low soil matric suctions (or nearly paddy condition) (2–33 kPa) and salinity (EC = 0.7–8 dS m^?1 for bean and 2–20 dS m^?1 for wheat) on the root respiration (Rr) in two sandy loam and clay loam soils at greenhouse condition. Results showed that the aeration porosity mainly controls Rr especially at 2 kPa matric suction. As matric suction increases, soil aeration rises and consequently the Rr reaches maximum values (7.9 μmol m^?3 s^?1 for bean and wheat) at 6 and 10 kPa suctions in clay loam and sandy loam soils, respectively. Using a mechanistic soil respiration model reveals that these matric suctions, h, are corresponded to the aeration porosities of 0.18 m³ m^?3 in sandy loam and 0.16 m³ m^?3 in clay loam soils. Bean and wheat Rr remains nearly constant at higher suctions (h > 10 kPa) in sandy loam and decreases slightly in clay loam soil. Gas diffusivity and the root surface area may explain the variation of the Rr between the sandy loam and the clay loam soils. Results showed that the salinity (EC = 6–8 dS m^?1 for bean and EC = 16–20 dS m^?1 for wheat) amplifies the effect of aeration stress at 2 kPa matric suction in both soils. We also observed a strong correlation between root surface area, Rs, and the Rr for all experiments. We concluded that the aeration deficit is not only major factor determining differential plant respiration under adverse stress conditions, and the salinity has a pronounced impact on differences in crop physiological responses.     

Applicability of APSIM to capture the effectiveness of irrigation management decisions in rice-based cropping sequence in the Upper-Gangetic Plains of India

Rice–wheat (RW) production system, which covers over 13.5 million ha in the Indo-Gangetic Plains of south Asia, is vital for food and nutritional security and livelihood of millions of poor people in this part of the region. Availability of irrigation water under projected climate change scenarios is a great concern, and demonstration of the impact of different irrigation regimes on rice, wheat, and system yields is essential to adopt suitable water saving technologies to minimize risk. This study tested the ability of the agricultural production systems simulator (APSIM) model to simulate the effects of different irrigation regimes on yield, irrigation water requirement, and irrigation water productivity (WP_i) of rice, wheat, and RW system in upper-gangetic plains of India. The long-term simulated rice yield showed a steadily declining trend at an average rate of 120 kg ha^?1 yr^?1 (R ² = 0.94, p < 0.05), while long-term simulated wheat yields showed a lower declining trend at an average rate of 48 kg ha^?1 yr^?1 (R ² = 0.48, p < 0.05). The highest WP_i of 8.31 kg ha^?1 mm^?1 was observed under RW system with the rice irrigation (IR) regime of 8 days alternate wetting and drying (AWD) and five irrigations for wheat with a yield penalty of 25.5 %. The next highest WP_i was observed in the treatment with a 5-day AWD regime in rice and five irrigations for wheat, with a yield penalty of 20.1 %. Thus, we can suggest that a 5-day AWD irrigation regime for rice combined with five irrigations during wheat could be the best option under water limiting situations.     

Effects of Different Soil Treatments on the Development of <Emphasis Type="Italic">Spongospora subterranea</Emphasis> f. sp. <Emphasis Type="Italic">subterranea</Emphasis> in Potato Roots and Tubers in the Greenhouse

Powdery scab caused by Spongospora subterranea f. sp. subterranea (Sss) causes extensive losses in potato production systems globally. Two pot experiments were established in the greenhouse in summer 2013 and winter 2014 to evaluate the effectiveness of different soil chemicals, fumigant, amendments and biological control agents (BCAs) against Sss in the rhizospheric soil, potato roots and tubers. The study used visual assessment methods to assess the effect of treatments on root galling and zoosporangia production, and qPCR to measure Sss concentration in the soil and in the potato roots and tubers. All six soil treatments, namely metam sodium, fluazinam, ZincMax, calcium cyanamide, Biocult and a combination of Bacillus subtilis and Trichoderma asperellum recorded significantly (P < 0.05) lower numbers of zoosporangia in the roots compared to the untreated control. The same effect was observed on the concentration of Sss DNA in the roots at tuber initiation. A more diverse picture was obtained when root gall scores at tuber initiation and Sss DNA in the rhizospheric soil at tuber initiation and harvesting were compared. Significant differences (P < 0.05) were also noted in disease severity, disease incidence, and tuber yield between metam sodium, fluazinam, ZincMax, calcium cyanamide and the untreated control. Calcium cyanamide gave the highest tuber yield. The study demonstrated the potential of soil treatments such as metam sodium, fluazinam, ZincMax and calcium cyanamide in managing Sss in potatoes by reducing the pathogen both in the rhizospheric soil and the roots of the potato plant.     

Short-term daily forecasting of crop evapotranspiration of rice using public weather forecasts

Accurate forecasts of daily crop evapotranspiration (ET_c) are essential for real-time irrigation management and water resource allocation. This paper presents a method for the short-term forecasting of ET_c using a single-crop coefficient approach and public weather forecasts. Temperature forecasts with a 7-day lead time in 2013–2015 were retrieved and entered into a calibrated Hargreaves–Samani model to compute daily reference evapotranspiration (ET₀) forecasts, while crop coefficient (K_c) empirical values were estimated from both observed ET_c value and calculated ET₀ values using the Penman–Monteith equation for the period of 2010–2012. Daily ET_c forecasts of irrigated double-cropping rice were determined for three growing seasons during the period of 2013–2015 and were compared with ET_c values measured by the weighing lysimeters at the Jiangxi experimental irrigation station in southeastern China. During the early rice season, the average mean absolute error (MAE) and root-mean-square-error (RMSE) values of ET_c forecasts ranged from 0.95 to 1.06 mm day^?1 and from 1.18 to 1.31 mm day^?1, respectively, and the average correlation coefficient (R) ranged from 0.39 to 0.54; for late rice, the average MAE and RMSE values ranged from 1.01 to 1.09 mm day^?1 and from 1.32 to 1.40 mm day^?1, respectively, and the average R value ranged from 0.54 to 0.58. There could be three factors responsible for errors in ET_c forecasts, including temperature forecast errors, K_c value errors and neglected meteorological variables in the HS model, including wind speed and relative humidity. In addition, ET_c was more sensitive to changes in temperature than K_c. The overall results indicated that it is appropriate to forecast ET_c with the proposed model for real-time irrigation management and water resource allocation.     

Chloropicrin Soil Fumigation Reduces <Emphasis Type="Italic">Spongospora subterranea</Emphasis> Soil Inoculum Levels but Does Not Control Powdery Scab Disease on Roots and Tubers of Potato

The effect of chloropicrin fumigation on the soil populations of Spongospora subterranea and the development of powdery scab, formation of root galls and tuber yield was investigated in seven field trials conducted in Minnesota and North Dakota. Sixteen potato cultivars, with different levels of susceptibility to disease on roots and tubers, were planted in plots treated with chloropicrin at rates ranging from zero to 201.8 kg a.i. ha^?1. The amount of S. subterranea DNA in soil was determined using qPCR. Bioassays were conducted to further assess the effect of chloropicrin fumigation on root colonization by S. subterranea in two potato cultivars with contrasting disease susceptibility. In the field, chloropicrin applied at rates between 70.1 to 201.8 kg a.i. ha^?1 significantly decreased S. subterranea initial inoculum in soil but increased the amount of disease observed on roots and tubers of susceptible cultivars. The effect of increasing disease was confirmed in controlled conditions experiments. Although the amount of S. subterranea DNA in roots of bioassay plants increased with increasing chloropicrin rates, it remained similar among potato cultivars. Chloropicrin fumigation significantly increased tuber yield which in cultivars such as Shepody and Umatilla Russet were associated with the amount root galls (r = 0.30; P < 0.03). Results of these studies contradict earlier reports on the use of chloropicrin fumigation for the control of powdery scab. Factors other than inoculum level, such as environmental conditions that affect inoculum efficiency and host susceptibility, may be significant contributors to the development of powdery scab and root gall formation.     

Mapping QTLs using a novel source of salinity tolerance from Hasawi and their interaction with environments in rice

Background

Salinity is one of the most severe and widespread abiotic stresses that affect rice production. The identification of major-effect quantitative trait loci (QTLs) for traits related to salinity tolerance and understanding of QTL × environment interactions (QEIs) can help in more precise and faster development of salinity-tolerant rice varieties through marker-assisted breeding. Recombinant inbred lines (RILs) derived from IR29/Hasawi (a novel source of salinity) were screened for salinity tolerance in the IRRI phytotron in the Philippines (E1) and in two other diverse environments in Senegal (E2) and Tanzania (E3). QTLs were mapped for traits related to salinity tolerance at the seedling stage.

Results

The RILs were genotyped using 194 polymorphic SNPs (single nucleotide polymorphisms). After removing segregation distortion markers (SDM), a total of 145 and 135 SNPs were used to construct a genetic linkage map with a length of 1655 and 1662 cM, with an average marker density of 11.4 cM in E1 and 12.3 cM in E2 and E3, respectively. A total of 34 QTLs were identified on 10 chromosomes for five traits using ICIM-ADD and segregation distortion locus (SDL) mapping (IM-ADD) under salinity stress across environments. Eight major genomic regions on chromosome 1 between 170 and 175 cM (qSES1.3, qSES1.4, qSL1.2, qSL1.3, qRL1.1, qRL1.2, qFWsht1.2, qDWsht1.2), chromosome 4 at 32 cM (qSES4.1, qFWsht4.2, qDWsht4.2), chromosome 6 at 115 cM (qFWsht6.1, qDWsht6.1), chromosome 8 at 105 cM (qFWsht8.1, qDWsht8.1), and chromosome 12 at 78 cM (qFWsht12.1, qDWsht12.1) have co-localized QTLs for the multiple traits that might be governing seedling stage salinity tolerance through multiple traits in different phenotyping environments, thus suggesting these as hot spots for tolerance of salinity. Forty-nine and 30 significant pair-wise epistatic interactions were detected between QTL-linked and QTL-unlinked regions using single-environment and multi-environment analyses.

Conclusions

The identification of genomic regions for salinity tolerance in the RILs showed that Hasawi possesses alleles that are novel for salinity tolerance. The common regions for the multiple QTLs across environments as co-localized regions on chromosomes 1, 4, 6, 8, and 12 could be due to linkage or pleiotropic effect, which might be helpful for multiple QTL introgression for marker-assisted breeding programs to improve the salinity tolerance of adaptive and popular but otherwise salinity-sensitive rice varieties.

     

Self-made microlysimeters to measure soil evaporation: a test on aerobic rice in northern Italy

Soil water balance researches aimed at improving crop water use efficiency often require the determination of soil evaporation. In this technical note, the performance of simple and cheap self-made microlysimeters for the measurement of soil evaporation was tested in an aerobic rice field managed by intermittent irrigation. Six microlysimeters obtained by cutting commercial PVC pipes and closing the bottom ends with caps were positioned in appropriate PVC outer casings installed into the soil. Three measurement campaigns (for a total of 11 measurement periods) were carried out in different vegetation cover conditions (rice development, rice maturity and after the crop harvest). Evaporation amounts were analysed with respect to climatic data, vegetation cover and soil water status and, finally, compared with the simulated results of a FAO Penman–Monteith “dual crop coefficient” model implemented with site-specific data. Evaporation rates in the three campaigns were closely dependent on the evaporative demand of the atmosphere ETo (R ² = 0.96, 0.98, 0.96), while the slope of the linear regression curve was strongly related to the vegetation cover (b = 0.29, 0.12, 0.94); soil water content, always rather high during the experiments, did not affect the evaporation rate. Measured and simulated evaporation amounts showed a close agreement: the linear regression was characterized by slope and R ² of 0.98 and 0.95, while RMSE and NSE indices were 0.15 and 0.94. From the tests conducted, we can conclude that the self-made microlysimeters presented in this note are affordable instruments for measuring soil evaporation, at least in temperate climate conditions.     

The Effect of Alfalfa Residue Incorporation on Soil Bacterial Communities and the Quantity of <Emphasis Type="Italic">Verticillium dahliae</Emphasis> Microsclerotia in Potato Fields in the Columbia Basin of Washington State,USA

Verticillium wilt, caused by the soil-borne fungus Verticillium dahliae, is one of the most important diseases of potato in North America. Soil incorporation of alfalfa residues prior to planting potato could be a nonchemical Verticillium wilt management tactic by reducing the number of viable microsclerotia in field soil. Verticillium dahliae microsclerotia were quantified in field soils where organic material from alfalfa was incorporated, and numbers of microsclerotia were compared to fields where alfalfa residue was not incorporated. In addition, bacterial metagenomics was utilized to characterize soils where organic material from alfalfa was or was not incorporated to determine if alfalfa residue incorporation facilitates the formation of soils that suppress or kill V. dahliae microsclerotia. The number of V. dahliae microsclerotia in soil was greater (P = 0.0003) in fields where crop residue was incorporated than fields without incorporation when chloropicrin was used as a fumigant. Conversely, the number of V. dahliae microsclerotia observed in potato plants did not differ (P = 0.4020) between fields where residues were or were not incorporated if chloropicrin was used. Alfalfa residue incorporation did not significantly alter the soil bacterial metagenome compared to fields not subject to residue incorporation in both years of study. Despite these conclusions, the method can be employed to analyze the effect of grower practices with the intent of linking a field practice to increasing soil bacterial diversity and decreasing Verticillium wilt severity on potato.     

Agronomic fortification of rice grains with secondary and micronutrients under differing crop management and soil moisture regimes in the north Indian Plains

Although the System of Rice Intensification (SRI) has been reported to produce higher paddy (Oryza sativa L.) yields with better-quality grains, little research has addressed the latter claim. This study investigated the interactive effects of rice cultivation methods with different irrigation schedules and plant density on the uptake and concentration of sulfur (S), zinc (Zn), iron (Fe), manganese (Mn) and copper (Cu) in the grain and straw of two rice cultivars during two rainy seasons in the northern plains of India. As the two seasons differed in amounts of rainfall, there were impacts of soil moisture differences on nutrient uptake. Plots with SRI cultivation methods enhanced the grain uptake and concentrations of S, Zn, Fe, Mn and Cu by 36, 32, 28, 32 and 63%, respectively, compared to conventional transplanting (CT). Under SRI management, the highest concentrations of S, Zn and Cu in the grain and straw occurred with irrigation intervals scheduled at 3 days after disappearance of ponded water (DADPW; 3_D), whereas Fe and Mn concentrations in the grain and straw were higher with irrigation at 1 DADPW (1 _D ) compared with plots under 3 _D or 5 DADPW (5 _D ). The higher nutrient uptakes were also manifested in higher grain yield in 1 _D and 3 _D plots (by 9 and 6%, respectively) compared with 5 _D . Wider spacing (25 × 25 cm) compared with closer spacing (20 × 20 cm) significantly increased yield and the uptake and concentrations of all the said nutrients in the grains. When comparing the performance of two cultivars, the total uptakes of Zn, Fe, Mn and Cu in both grain and straw were significantly more in Hybrid 6444 than the improved variety Pant Dhan 4. Overall, SRI crop management compared to CT practices led to more biological fortification of rice grains with respect to S and the four micronutrients studied, giving a concomitant yield advantage of about 17% on average in this region.     

National risk assessment of irrigation on farmland near wastewater treatment plants in Korea

There are more than 130 agricultural water sources that are located near wastewater treatment plants (WWTPS) in Korea. The majority of the stream flow in these farmlands is dependent on effluent from the WWTPS during the dry season. This explains the indirect or direct reuse of effluent from WWTPs. Most of the farm workers use the effluent without any additional treatment because they have a lack of knowledge regarding water reuse. In addition, insufficient consideration is given to health and hygiene safety. This study reviewed the safety issues in these farmlands. A total of 53 farmlands located near WWTPs were investigated to determine if farm workers used effluent as irrigation water on their paddy rice fields. Total coliform, fecal coliform, Escherichia coli (E. coli), and the concentration of some heavy metals in paddy water and soils were measured. Quantitative microbial and toxic risk assessment methods were used to review the safety of wastewater irrigation. E. coli concentrations were used to estimate the microbial risk of enteric disease in the paddy fields. The microbial risk was 5.9 × 10^?4, which did not satisfy the minimum safety standards. Carcinogenic risk was 3.99 × 10^?5 and non-carcinogenic risk was 6.34 × 10^?1. These values were too high to be considered safe, even though the measurements of E. coli and some toxic metals were of short duration.     

Rooting Characteristics of <Emphasis Type="Italic">Solanum chacoense</Emphasis> and <Emphasis Type="Italic">Solanum tuberosum in Vitro</Emphasis>

The precise level of environmental control in vitro may aid in identifying genetically superior plant germplasm for rooting characteristics (RC) linked to increased foraging for plant nitrogen (N). The objectives of this research were to determine the phenotypic variation in root morphological responses of 49 Solanum chacoense (chc), 30 Solanum tuberosum Group Phureja – Solanum tuberosum Group Stenotomum (phu-stn), and three Solanum tuberosum (tbr) genotypes to 1.0 and 0.5 N rate in vitro for 28 d, and identify genotypes with superior RC. The 0.5 N significantly increased density of root length, surface area, and tips. All RC were significantly greater in chc than in either phu-stn or tbr. Based upon clustering on root length, surface area, and volume, the cluster with the greatest rooting values consisted of eight chc genotypes that may be utilized to initiate a breeding program to improve RC in potato.     

Effects of various organic amendments on organic carbon pools and water stable aggregates under a scented rice–potato–onion cropping system

Pools of organic carbon are quantified from the soil samples under scented rice crop from different soil layers (0–10, 10–20, and 20–30 cm) under 9 years’ long-term trials with five treatments in scented rice–potato–onion cropping system. These treatments were 100 % NPK (NPK), 50 % recommended NPK through mineral fertilizers + 50 % N as FYM (NPK + FYM), FYM + vermicompost (VC) + neem cake (NC) each equivalent to one-third of recommended N (FYM + VC + NC), 50 % N as FYM + biofertilizer for N + bone meal to substitute phosphorus requirement of crops + phosphate solubilizing bacteria (FYM + BFN + BM + PSB), FYM + vermicompost + neem cake each equivalent to 1/3rd of recommended N + PSB (FYM + VC + NC + PSB). SMBC (479 mg kg^?1), HWEOC (373 mg kg^?1), CWSCHO (235 mg kg^?1), HWSCHO (839 mg kg^?1), and ASCHO (180 mg kg^?1) were found to be the highest in the soil treated with FYM + VC + NC + PSB and the lowest with NPK. The quantity of hot water-extractable carbohydrate content is highest amongst cold water, dilute acid and hot water extractable carbohydrate that decreases with the soil depth irrespective of treatments, except CWEOC. Soil microbial biomass carbon (SMBC) shows significant correlation with CWEOC (r = 0.60**), HWEOC (r = 0.94**), CWSCHO (r = 0.75**), HWSCHO (r = 0.83**), and ASCHO (r = 0.83**) that primed for better aggregate stability irrespective of soil layers up to 30 cm depth. This indicates that labile carbon pools, most specifically water-soluble carbon, carbohydrate, microbial biomass, could be a suitable indicator for evaluation of soil quality, particularly in relation to soil aggregation.     

Feasibility and sizing of unlined on-farm pond for partial rice substitution in rainfed uplands of eastern India

Rice (Oryza sativa L.)-dominated rainfed uplands of eastern India are facing two major problems such as lack of irrigation water sources and low productivity. In fact, unlined on-farm pond (OFP) technology in this terrain could prove to be an effective agricultural drought mitigation measure for monsoon crops but failed to ensure supplemental irrigation (SI) to the next winter crops. Consequently, the OFP technology could not pick up in the region. In order to overcome the shortcomings in the technology, the location of the OFP has been changed and a new concept of partial rice substitution (PRS) has been introduced in the present study to provide SI for the second crop in winter. Maize (Zea mays L.) and rice crops at upper and lower compartments of the field, respectively, were tried at various crop substitution ratios (CSR) of 70:30, 60:40, 50:50, 40:60, and 30:70 during monsoon season. Rainfall excess from both the compartments was harvested in an unlined OFP located in between the compartments and used for providing SI. In winter, black gram (Vigna mungo L.) and mustard (Brassica campestris) were taken in the upper and lower compartments, respectively, based on availability of water in the OFP. Water balance models were used to simulate the soil moisture in crop root zone as well as storage of water in the OFP. The optimal size of such unlined OFP for average land holdings (1200 m²) in rainfed uplands and under 60:40 CSR was found to be occupying 6 % area of the crop field.     

Maturity-Adjusted Resistance of Potato (<Emphasis Type="Italic">Solanum tuberosum</Emphasis> L.) Cultivars to Verticillium Wilt Caused by <Emphasis Type="Italic">Verticillium dahliae</Emphasis>

Verticillium wilt is a fungal disease of potato caused by two species of Verticillium, V. dahliae and V. albo atrum. The pathogen infects the vascular tissue of potato plants through roots, interfering with the transport of water and nutrition, and reducing both the yield and quality of tubers. We have evaluated the reaction of 283 potato clones (274 cultivars and nine breeding selections) to inoculation with V. dahliae under greenhouse conditions. A significant linear correlation (r = 0.4, p < 0.0001) was detected between plant maturity and partial resistance to the pathogen, with late maturing clones being generally more resistant. Maturity-adjusted resistance, that takes into consideration both plant maturity and resistance, was calculated from residuals of the linear regression between the two traits. Even after adjusting for maturity, the difference in the resistance of clones was still highly significant, indicating that a substantial part of resistance cannot be explained by the effect of maturity. The highest maturity-adjusted resistance was found in the cv. Navajo, while the most susceptible clone was the cv. Pungo. We hope that the present abundance of data about the resistance and maturity of 283 clones will help potato breeders to develop cultivars with improved resistance to V. dahliae.     

Prediction of water quality effect on saturated hydraulic conductivity of soil by artificial neural networks

This study was conducted to investigate the impact of water salinity (EC_w) and sodicity (SAR_w) on saturated (K_s) and relative (K_r) hydraulic conductivities in two clay (C) and sandy clay loam (SCL) soils. The results showed that the K_s decreased with increasing SAR_w, and in all of water quality treatments, the K_s of SCL soil was higher than that of the C soil. Sodicity effect (even at high SAR_w) on the K_r of clay soil was minimized by high salinity. Although K_r of both soils similarly responded to EC_w and SAR_w, microstructure of clay soil was more sensitive to water quality. Effect of EC_w on soil structure was greater than that of SAR_w. In order to assess the applicability of artificial neural networks (ANNs) in estimating K_s and K_r, two types of FFBP and CFBP ANNs and two training algorithms, namely Levenberg–Marquardt (LM) and Bayesian regulation, were employed with two strategies of uniform threshold and different threshold functions. Multiple linear regressions were also used for K_s and K_r prediction. Based on the ANN results of second strategy, best topology (4–5–4–1) was belonged to CFBP network with LM algorithm, LOGSIG–LOGSIG–TANSIG threshold functions, and values of MAE and R² are equal to 0.1761 and 0.9945, respectively. Overall, the efficacy of ANNs is much greater than regression method for K_s prediction.     

Variation in the Carotenoid Composition of the Lycopene-Rich Brazilian Fruit <Emphasis Type="Italic">Eugenia uniflora</Emphasis> L.

The indigenous pitanga (Eugenia uniflora L.) is now marketed and commercially processed in Brazil. In the present work, the carotenoids of the pitanga fruit from two states and at two stages of ripening, as well as of processed pitanga products (frozen pulp and juice, the brands being designated as A, B and C) commercialized in Campinas, São Paulo, were determined by HPLC. As compared to ripe pitanga from Medianeira, Paraná, those from Campinas had significantly higher (all-E)-lycopene (14.0 vs. 71.1 μg/g), (13Z)-lycopene (1.1 vs. 5.0 μg/g) and (all-E)-γ-carotene (1.6 vs. 3.8 μg/g) levels. Significant increases in most of the carotenoids occurred from the partially ripe to the ripe fruits, with (all-E)-lycopene doubling its concentration in fruits from both states. Pitanga was found to be one of the richest fruit sources of carotenoids, particularly lycopene, but the processed products had much lower lycopene content. The mean (all-E)-lycopene concentration was 16.6 μg/g for frozen pulp brand A, 23.0 μg/g for bottled juice brand B and 25.6 μg/g for bottled juice brand C. Optimization of processing is therefore needed to guarantee better retention of this important carotenoid.