超声波辅助提取大豆总木脂素及其含量分析

以乙醇为浸提溶剂,超声波辅助提取不同品种大豆中的木脂素,并用紫外-可见分光光度法对其含量进行分析,同时对浸提溶剂、料液比、浸提时间及次数等因素进行比较。结果表明:以乙醇为浸提溶剂,料液比在1/8~1/12范围,浸提时间20 min,浸提2次效果比较理想;标准对照品在0~0.18 mg.mL-1浓度范围内吸光度与其总木脂素含量呈良好的线性关系,回归方程A=6.081c+0.0445,相关系数r=0.9998,总木脂素检出限8.3×10-5mg.mL-1,平均回收率为94.4%,RSD为1.2%。该方法简便、实用、可靠,可对大豆木脂素的深入研究提供参考。     

北五味子根、茎、叶与果实木脂素成分比较研究

本文利用薄层扫描法测定了北五味子根、茎、叶与果实中木脂素的组成及含量.结果表明,茎与果实中总木脂素的组成及含量相近,有开发价值.     

响应曲面优化加压溶剂提取五味子中4种木脂素

利用响应曲面法(Response surface methodology,RSM)建模和优化加速溶剂萃取(Accelerated solvent extraction,ASE)五味子中4个木脂素。基于RSM方法 ,利用Box-Behnken设计(Box-Behnken design,BBD)获得了ASE最佳的提取条件。结果表明:ASE最佳提取4个木脂素的条件是87%的乙醇作为提取溶剂,提取温度为160℃,静态萃取时间为10min,提取压力1500 psi,1个提取循环,60%冲水量。在最佳工艺条件下,4个木脂素的实测值14.72mg/g,与模型预测值基本接近。     

高效液相色谱法同时测定五味子中8种木脂素含量

目的建立并优化一种能够同时测定五味子药材中8种木脂素含量的高效液相色谱方法,为五味子药材的质量控制方法研究提供依据。方法采用高效液相色谱方法,同时测定五味子中五味子醇甲、五味子醇乙、当归酰基戈米辛H、五味子酯甲、五味子酯乙、五味子甲素、五味子乙素和五味子丙素含量,通过专属性、线性、稳定性、精密度和回收率实验进行方法学验证,并利用该方法对20批不同产地的五味子药材进行木脂素含量的测定。结果建立的高效液相色谱检测方法专属性强、准确度和精密度高,线性和稳定性好,能够应用于木脂素类化合物的含量测定。结论本研究所建立的木脂素类化合物含量测定方法为综合评价药材的质量、完善五味子质量标准提供科学依据。     

亚麻品种及生态环境对亚麻木脂素含量的影响

亚麻木脂素只在亚麻籽皮中存在,其它器官中没有发现。亚麻不同品种和生态环境对亚麻籽中木脂素的含量影响很大,影响程度依次为品种、种植区域和年份。在检测的我国亚麻主栽品种中晋亚9号的亚麻木脂素含量最高,达到20.05 mg/g。     

染料木素和大豆素的结构分析

染料木素和大豆素是大豆异黄酮苷元的两种主要成分,具有多种生物活性,关于其混合物的同时结构分析,鲜有报道.实验为确保混合物结构解析的正确性,利用质谱技术得出的物质分子离子峰及相关特征碎片峰、红外光谱技术所得的特征官能团信息、核磁共振(氢谱、碳谱及二维核磁共振谱等)技术分析出的碳氢等元素的结构分布情况,从化学式量、主要官能团及具体分子结构等方面成功地对染料木素和大豆素的混合物进行了分子结构的同时佐证解析.这避免了实验必须对}昆合物进行分离收集,再对各物质单独结构分析所带来的繁琐技术操作,使得方法简便,快捷省时.同时,实验所得方法为其他混合物结构解析提供技术参考.     

染料木素联合多不饱和脂肪酸对MCF-7细胞脂代谢基因表达的影响

选用不同浓度的染料木素(GEN)及二十二碳六烯酸(DHA)作用于MCF-7细胞,研究大豆中抗癌成分染料木素与不同比例的多不饱和脂肪酸(PUFAs)联用,对乳腺癌细胞MCF-7增殖及脂代谢基因表达情况的影响。根据DHA的有效浓度设定亚油酸(LA)及LA∶DHA(10∶1)混合PUFAs作用浓度。将GEN分别与DHA、LA及LA∶DHA(10∶1)联合作用细胞,检测细胞生存率,并通过qPCR方法检测细胞内过氧化物酶体增殖物激活受体γ(PPARγ)、5-脂氧合酶(5-LOX)、环氧合酶-2(COX-2)mRNA表达情况。结果表明:高浓度GEN(300,200,100μmol·L~(-1))及DHA(400,300,200μmol·L~(-1))可抑制MCF-7细胞的增殖,且随着时间增加抑制作用增强。而低浓度GEN(50,25μmol·L~(-1))及LA组及10∶1组对细胞增殖有轻度促进作用。添加GEN的G+L组和G+10∶1组细胞的生存率显著降低,同时GEN可降低G+L组细胞5-LOX mRNA的表达量,且GEN与LA∶DHA(10∶1)联用,增加了细胞内PPARγ mRNA的表达水平并抑制了COX-2 mRNA的表达。试验证实GEN可抑制高比例ω-6 PUFAs对乳腺癌细胞增殖的促进作用,其作用机制主要是通过增加PPARγ mRNA表达,抑制COX-2 mRNA表达水平实现的。大豆的抗乳腺癌作用可能是由于GEN与PUFAs在抑制乳腺癌细胞增殖方面产生了协同效应。     

染料木素锌、铜金属配合物的制备及抗癌活性研究

为研究大豆中异黄酮类成分染料木素锌、铜金属配合物的制备方法及其抗癌活性,以染料木素为配体,设计并合成染料木素-锌、染料木素-铜配合物,利用红外光谱、紫外光谱、热重分析等方法对配合物进行表征,并通过MTT法考察染料木素及其锌、铜金属配合物对人非小细胞肺癌细胞A549、人乳腺癌细胞MCF?7及人脐静脉内皮细胞HUVEC的增...     

多年生野生大豆的生物学性状及叶片中大豆苷元和染料木素含量分析

以引进的78份多年生野生大豆为材料,在上海繁殖时,观察了形态性状、测定其叶片中大豆苷元及染料木素的含量,旨在明确变异范围和特点,筛选优异材料,为多年生野生大豆资源的研究与利用提供理论依据.在供试的多年生野生大豆中,Glycine falcata种有5份材料表现较好;Glycine tabacina种有9份材料大豆苷元和染料木素的含量最高.根据形态性状和大豆叶片异黄酮含量的综合表现,发现Glycine falcata种是异黄酮含量较高的优异多年生野生大豆资源.     

从银合欢种子提取高统纯度含羞草素的研究

叙述了从银合欢种子提取高纯度含差羞的草素的一种较简易的方法。该法是用水为溶剂，在６０℃进行提取，然后经纯化处理和重结晶。含羞草素的相对提取率为２４．１％￣２７．８％，纯度在９５％以上。影响提取率的关键性因素是浓缩程度，产品的主要杂质是游离氨基酸或短肽。     

Effects of different extraction methods on the structural properties and bioactivities of polysaccharides extracted from Qingke (Tibetan hulless barley)

In this study, four extraction methods, including ultrasonic assisted extraction (UAE), hot water extraction (HWE), microwave assisted extraction (MAE), and pressurized water extraction (PWE), were used to extract Qingke polysaccharides (THBs), and their physicochemical structures, in vitro antioxidant activities, and in vitro hypolipidemic activities were investigated and compared. Results demonstrated that the yields, the chemical compositions, the apparent viscosities, the molecular weights, and the molar ratios of constituent monosaccharides of THBs varied by different extraction methods. THB-P extracted by PWE possessed the highest molecular weight and apparent viscosity among all tested THBs. In addition, all THBs extracted by different methods exerted obvious in vitro antioxidant activities, in vitro binding capacities, and inhibitory effects on the pancreatic lipase. Indeed, both antioxidant activities and hypolipidemic activities of THB-P were higher than that of THB-H, THB-U, and THB-M obtained by HWE, UAE, and MAE, respectively, which might be due to the high molecular weight and apparent viscosity of THB-P. The findings indicated that the pressurized water extraction could be a convenient method for the extraction of polysaccharides from Qingke with high biological activities for applications in the functional food fields.     

普洱茶化学成分及其生物学功效

普洱茶独特的文化背景和品质引起了人们的极大关注,但有关普洱茶的研究还刚刚起步。本文从化学成分和功效方面综述了普洱茶近年来的研究进展。提出了今后的研究展望。     

油莎豆的研究现状及展望

介绍了油莎豆抗逆性强、播种期长、产量高、块茎含油率高等生物性状,以及能做绿肥、保健食用油、保健医药、饲料等用途,并对油莎豆未来大面积种植推广进行了展望。     

甜菜根腐病的生物和化学控制初步研究

采用小区和大区对比相结合的试验方法,探讨了生物和化学药剂防治甜菜根腐病的效果。研究结果表明,５０％福美双可湿性粉剂与７０％土菌消可湿性粉剂等量混合后种子重量的０．８％拌种、荧光假单胞菌１００倍液浸种２４ｈ、５％菌毒清水剂３００倍液浸种２４ｈ与５０％福美双可湿性粉剂种子重量０．８％拌种（对照）比较,防治甜菜根腐病效果均达５０％以上,差异显著,并且具有兼防甜菜立枯病和明显的增产增糖作用     

Bioactive Compounds and Health-Promoting Properties of Berry Fruits: A Review

This study characterizes biologically active compounds of berry fruits, including non-nutritive compounds such as phenolic compounds, including anthocyanins, phenolic acids, stilbens and tannins, as well as nutritive compounds such as carotenoids and vitamin C. It discusses the biological activity of those compounds, in particular their antioxidant properties and the resulting health benefits.     

富硒灵芝的研究进展

富硒灵芝是以灵芝作为硒的转化载体培养获得的,其兼具灵芝与硒的生物活性,具有很高的药用价值对近年来对富硒灵芝的研究作一综述,包括灵芝的富硒培养、富硒灵芝的生物活性、含硒有效成分的分离和分析以及富硒灵芝产品开发的现状等,并对其研究方向进行了展望、     

The impact of cellulose fibre surface modification on some physico-chemical properties of the ensuing papers

This review covers both physical and chemical modifications of cellulose fibre surface and their impact on the properties of the ensuing papers. It is not restricted to actual industrial operations, but also deals instead with ongoing research in this area, which appears promising in terms of possible applications to papermaking.     

Evaluation of spray programmes for the management of leaf spot incited by Pseudomonas syringae pv. syringae on tomato cv. Cuore di bue

Four experimental trials were carried out in northern Italy under greenhouse conditions against bacterial leaf spot of tomato, incited by Pseudomonas syringae pv. syringae, a disease recently observed in the country. The efficacy of spray programmes, based on the use of several copper-based compounds, acibenzolar-S-methyl, fosetyl Al and two biological control agents, Bacillus subtilis strain QST 713 and Pseudomonas chlororaphis MA 342, was tested. The best results were obtained with the alternation of copper oxychloride, used alone or in mixture with acibenzolar-S-methyl, with acibenzolar-S-methyl, applied for a total of two sprays. Acibenzolar-S-methyl applied alone was also effective. However, due to its possible slight phytotoxic effect, observed in our case in one trial, strategies relying on a limited number of its application are preferred. The efficacy of acibenzolar-S-methyl is higher under lower inoculum conditions. Among the tested copper-based fungicides, copper oxychloride provided the best and most consistent results, with no or limited phytotoxicity. Other copper compounds were much less effective and sometimes caused a reduction in plant height. The disease suppression achieved with B. subtilis strain QST 713 was only partial, while P. chlororaphis was not effective. The advantages of programmes based on rotation of copper compounds and acibenzolar-S-methyl are discussed.     

附子理中片中人参皂苷的最佳生产工艺研究

目的：在保证原工艺基本稳定的前提下,对附子理中片中人参提取时间进行优化。方法：以人参皂苷Re的含量为检测指标,采用高效液相色谱法测定各样品的含量,结合浸膏收率进行综合评价,确定最佳的提取时间。结果：浸膏收率和人参皂苷Re含量相对较高的两次煎煮时间分别为135min、75min。结论：该研究方法简便,准确,优化得到的工艺可以用于大工业生产。     

大豆异黄酮的生物活性及毒理学研究进展

大豆异黄酮具有预防和治疗妇女更年期综合征、心血管疾病和骨质疏松症等多种生物学活性,并对多种肿瘤如乳腺癌、前列腺癌结肠癌等具有预防和抑制作用.但作为一种重要的植物雌激素,近年来其毒理学作用尤其是对遗传物质以及人类生长发育的影响受到了广泛的关注.文章介绍了大豆异黄酮的重要生物活性并着重综述了大豆异黄酮的毒理学研究进展.