白梭梭幼苗生长和生物量分配对不同盐分类型和浓度的响应

采用盆栽实验,设置3种盐分类型（NaCl,Na₂SO₄以及两者等摩尔比例混合盐）和6个浓度梯度〔0（CK）,50,100,200,300,500 mmol/L）〕,探讨了白梭梭（Haloxylon persicum Bunge ex Boiss. et Buhse）幼苗生长以及不同生长期地上地下生物量分配,对不同盐分类型及浓度的响应。结果发现：① 不同盐分类型和浓度处理下,白梭梭幼苗同化枝直径和分叉数具有明显差异,幼苗株高、基径和同化枝枝长随盐分浓度增大而减小,适度盐分浓度（300 mmol/L）促进主根生长,高盐分浓度（500 mmol/L）则具有显著抑制作用。② 随处理时间和盐分浓度的增加,白梭梭幼苗生物量积累受到的抑制不断增大,与对照相比,处理后期（第75 d）500 mmol/L浓度的NaCl,Na₂SO₄和混合盐处理下,白梭梭幼苗总生物量分别降低77%,87%和78%,地上生物量分别降低78%,87%和74%,地下生物量分别降低75%,81%和76%。③ 随时间的延长,白梭梭幼苗根冠比在各盐分处理下均呈增大趋势,但随盐浓度增加,根冠比变化趋势随盐分类型和处理时间的不同而有所区别。④ 白梭梭幼苗地上-地下生物量存在明显异速生长关系,且盐分处理未显著改变异速生长指数,均表现为地下生物量累积速率大于地上生物量。研究结果表明,在盐分胁迫下,白梭梭幼苗地上地下生物量分配对Na₂SO₄作出的响应要先于NaCl和混合盐,随处理时间的延长,对3种盐分类型作出的响应趋于一致,即将更多的生物量分配给地下根系,以缓解盐分过多造成的水分短缺等影响,维持植物的存活,符合最优分配理论。     

樟子松幼苗水力结构参数和生长特征对模拟降水梯度的响应

水分是影响樟子松生长和存活的关键因素,为弄清樟子松幼苗对水分胁迫的响应过程,采用人工控制降水处理方法 (105%年均降水量：500 mm,无干旱; 74%年均降水量: 350 mm,轻度干旱;42%年均降水量: 200 mm,干旱),监测了2 a生樟子松幼苗水力结构和生长特征对降水处理的响应。结果表明：随着降水量减少,幼苗水分饱和亏缺没有发生显著变化;轻度干旱下幼苗组织密度显著增加,干旱下幼苗失水系数显著降低;表明樟子松幼苗的保水、抗旱能力随干旱胁迫程度增强而增加。干旱胁迫显著降低了株高、基径和各器官生物量的增加量,轻度干旱胁迫下幼苗根重比和根冠比显著增加,表明轻度干旱胁迫改变了生物量分配格局,促使幼苗向有利于水分维持方向的发展策略;与轻度干旱相比,干旱胁迫下幼苗生物量分配策略并没有发生明显变化。樟子松幼苗可以通过改变水力结构特征和生物量分配策略来适应轻度干旱;而在干旱下幼苗仅改变水力结构特征,不利于其在干旱环境下生存。     

白茎盐生草幼苗对干旱胁迫的响应研究

采用盆栽试验对白茎盐生草幼苗进行为期0d、5d、10d、15d、20d、25d的自然失水胁迫,通过生理生长指标和叶片微观结构研究白茎盐生草幼苗对干旱胁迫的响应,为干旱半干旱区水土保持和生态恢复提供理论基础。研究表明:随干旱程度的加深,土壤含水率、白茎盐生草叶片肉质化程度、叶片相对含水量明显下降,而根冠比、质膜相对透性、株高、冠幅呈现不同程度的上升趋势。对白茎盐生草叶片进行扫描电镜观察发现:随着干旱天数的增加,气孔长度、气孔宽度、气孔开度减小、保卫细胞增厚、而气孔密度变化不明显。结论:白茎盐生草幼苗通过增大根冠比和质膜相对透性,减小叶片肉质化程度、叶片相对含水量,株高和冠幅的增长量也减小,减小气孔长度、气孔宽度、气孔开度来适应干旱胁迫。     

干旱胁迫对玉米苗期植株生长和保护酶活性的影响

以2个不同抗旱性玉米品种云瑞47(抗旱性强)和云瑞88(抗旱性弱)为材料,采用盆栽控水试验,设置轻度干旱胁迫(LS)、中度干旱胁迫(MS)、重度干旱胁迫(SS)3个干旱处理和正常灌水(CK),研究了干旱胁迫对2个玉米品种苗期株高、叶面积、生物量、根冠比和保护酶活性的影响,以探讨干旱胁迫对玉米苗期生长发育及其生理过程的影响机制。结果表明:(1)干旱胁迫抑制2个玉米品种苗期植株生长,导致植株株高降低、叶面积减少、整株生物量显著下降,根冠比增加,且抗旱性弱的品种云瑞88对干旱胁迫的敏感性高于抗旱性强的品种云瑞47。(2)干旱胁迫下2个玉米品种叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性表现为先升高后降低趋势,在MS处理下达到最高。与CK相比,云瑞47叶片SOD、POD和CAT分别增加了27.04%、16.95%和19.13%;云瑞88叶片SOD、POD和CAT分别增加了22.55%、11.20%和12.64%;(3)干旱胁迫增加了2个品种叶片丙二醛(MDA)含量,随着干旱胁迫程度的加剧,云瑞47(耐旱性强)叶片MDA变化幅度小于云瑞88(耐旱性弱),尤其SS处理下云瑞88的MDA积累量是云瑞47的1.31倍。云瑞47的各生理参数比云瑞88受旱影响小,干旱胁迫下仍具有较强的保护酶活性和较低的MDA含量,是云瑞47适应干旱环境的主要生理原因。     

光照对胡杨幼苗定居初期生长状况和生物量分配的影响

在自然条件下通过全自然光和遮光（20%自然光）处理研究胡杨（Populus euphratica Olivier)小幼苗生物和生物量分配对光因子的响应，实验表明光照对根冠比，叶面积比率和根重比率没有显著影响，光因子不改变胡杨幼苗生物量的分配状况，高光照能明显促进根的伸长，叶面积的增加和生物量的积累，遮荫环境则会阻碍胡杨幼苗的生长。     

水分和温度对紫茎泽兰幼苗生长的影响

比较2个水分和3个温度梯度条件下,中国和墨西哥两种群紫茎泽兰（Eupatorium adenophorum Spreng）的形态、生物量分配和光合色素的可塑性反应,探讨两种群在幼苗阶段生长特点及入侵潜力。结果表明：（1）各温度水分处理下墨西哥种群株高、叶片数、总叶面积、总生物量、叶生物量比、叶根比、总叶绿素较高,中国种群根长、根生物量、根冠比较高。表明墨西哥种群在适宜的环境条件下比中国种群有更高的资源捕获能力,而中国种群对干旱和低温条件有更好的适应能力。（2）两种群紫茎泽兰对水分响应显著,100%水分下总叶面积、平均叶面积、总生物量、叶生物量比、叶面积比、叶根比、总叶绿素均高于50%水分条件。对温度响应显著,尤其20℃时,中国种群根长,墨西哥种群总叶面积,100%水分下平均叶面积、总生物量、叶生物量比、叶根比5,0%水分下根生物量比,均达最高值。两种群喜湿润的环境,温度20℃时生长较好。     

水分胁迫下荒漠地区2种草本植物生物量分配策略

水分胁迫是荒漠地区生态系统结构和功能变化的主要驱动力。分析水分胁迫下,不同生长阶段荒漠植物生物量在地上-地下的分配比例,对精确估算地下有机碳存储量有重要的理论意义。通过盆栽控制实验,设置对照、中度和重度胁迫的水分梯度,选取荒漠地区2种草本植物涩荠（Malcolmia africana）和角果藜（Ceratocarpus arenarius）,分析其生物量在地上-地下的分配比例变化。结果表明：无论水分胁迫存在与否,总生物量累积均符合Sigmoidal生长模型;水分胁迫对植株早期发育阶段总生物量的累积无明显影响,但显著减少了发育后期总生物量的累积;植株每生长10 d,涩荠和角果藜叶片占总生物量的比例分别减少8.9%～10.6%和3.1%～3.4%。几乎等同于茎所增加的比例。水分胁迫使得地下生物量显著增加,但总生物量的累积仍以地上部分为主;根冠比在整个生长过程中呈4个渐变的阶段。     

艾比湖自然保护区盐生植物的分布及其水分利用效率

以艾比湖自然保护区内盐生植物为研究对象,采用野外调查与室内实验相结合的方法,揭示土壤盐含量与盐生植物数量分布及水分利用效率之间的关系。结果表明:(1)采集的9个土壤样地可划分成高、中和低盐度区3种类型;(2)拒盐和泌盐植物的丰富度在土壤盐分梯度上没有显著性差别,稀盐植物的丰富度在高盐度区显著高于中、低盐度区(高中低)(P0.05),拒盐、泌盐和稀盐植物的多度在中盐度区均显著高于高、低盐度区(中高低)(P0.05)。在高、中盐度区,盐生植物的多度和丰富度为:稀盐拒盐泌盐植物;相反,在低盐度区,泌盐拒盐稀盐植物;(3)稀盐和泌盐植物的δ~(13)C值在盐分梯度上存在显著性差异(P0.05);拒盐植物的δ~(13)C值在土壤盐分梯度上无显著性差异(P0.05);(4)中盐度区泌盐植物的δ~(13)C值显著低于拒盐和稀盐植物外(P0.05),在高盐度和低盐度区,3种盐生植物的δ~(13)C值没有显著性变化(P0.05);(5)拒盐、稀盐和泌盐植物的脯氨酸、可溶性蛋白质、可溶性糖和硝酸还原酶活性在3个土壤盐分梯度上均无显著性差异(P0.05);(6)同土壤盐分条件下,除可溶性蛋白质、可溶性糖和硝酸还原酶活性在少数情况下3种盐生植物间有差别外,大多情况下植物抗胁迫生理指标在3种盐生植物间没有显著性变化(P0.05);(7)抗胁迫生理指标、δ~(13)C值、多度和丰富度之间的关系随盐生植物种类迥异。综合以上分析,盐生植物在土壤盐分梯度上的分布、水分利用效率均与盐生植物的类型有关,其变化可能受内部生理调节、盐分的运输规律等多种因素的综合影响。     

4种盐生植物生长对土壤石油污染的响应

为了探讨4种新疆盐生植物在石油污染土壤上的生长适应性及修复效果,设置了5种不同浓度的石油污染处理,对4种供试盐生植物的株高、鲜重、根冠比及土壤中总石油烃含量进行了观测。结果表明：① 当土壤石油污染浓度为0.5%时,对4种供试植物的生长均有一定的促进作用,其生长指标均大于空白对照组,但差异不显著。② 随着污染物浓度的增大,4种供试植物的生长也受到了显著的抑制作用。其中,盐角草与野榆钱菠菜幼苗在生长过程中,当土壤石油污染浓度分别为1.5%和2%时,出现叶片枯黄死亡现象,其生长期只能维持20 d左右。③ 经过120 d的修复,4种供试植物对土壤中石油烃类污染物都有一定的修复作用,其总石油烃降解率为：鞑靼滨藜﹥盐地碱蓬﹥野榆钱菠菜﹥盐角草。④ 与其他2种植物相比,鞑靼滨藜和盐地碱蓬具有较大的石油污染耐受性和修复效果,表明其具有修复石油污染土壤的应用潜力。     

3种棒果芥属植物生物量分配及异速生长分析

类短命植物是准噶尔荒漠早春草本植物类群的重要而独特的组成部分,研究其生物量分配和异速生长关系,有助于深入了解类短命植物的生存策略与生态功能。以棒果芥（ Sterigmostemum tomentosum ）、福海棒果芥（ S ． fuhaiense ）和黄花棒果芥（ S ． sulfureum ）为研究对象,采用挖掘法获取野外成株全株生物量,对三者的器官生物量、分配比例及异速生长关系进行了对比分析。结果表明,3种植物器官生物量及其分配比例均差异明显。福海棒果芥生物量最大,但根冠比（R／S）和叶冠比（L／S）最小;棒果芥生物量最小,但L／S最大;黄花棒果芥则具有最大的R／S。棒果芥和黄花棒果芥的根冠比（R／S）均随个体的增大而显著下降,表明二者地上（AGB）与地下生物量（BGB）分配受到个体大小的强烈影响。福海棒果芥AGB－BGB间为等速生长关系（幂指数α＝1）,而另外2种均为异速生长关系;棒果芥、福海棒果芥的叶生物量（LB）与AGB间为等速生长关系,而黄花棒果芥符合异速生长关系（α＜1）;3个物种的LB－BGB间具有共同的异速生长指数（0．816）,表现出强烈的功能趋同性。总之,3个物种间的生物量分配及异速生长关系没有一致规律,但体现了类短命植物生物量分配的特点。     

羊草干物质积累及叶片气体交换对土壤水分的响应

采用盆栽方法模拟羊草干物质积累和叶片气体交换参数对 5个水分梯度的响应表明 :轻度、中度干旱与对照相比 ,羊草叶片的相对含水量 (RWC )有所升高 ;严重和极严重干旱使RWC显著降低。干旱降低植株生物量 ,后期的降低幅度远远大于前期 ,反映出羊草叶片水分状况对适度的土壤干旱具有良好的适应性 ,而过度干旱对羊草的生长造成威胁。鞘的分配随时间呈下降趋势 ,而根茎的趋势与鞘相反 ,表明鞘的暂储物质有可能向根茎转移。干旱促进早期根的分配和根冠比增加 ,后期降低 ,表明羊草在受到持续干旱后 ,通过增加根部的比重来提高抗旱性的能力逐渐降低。干旱降低了光合速率 ,增加了胞间CO2 浓度 ,但对水分利用率 (WUE )的影响随日进程而波动 ,在上午 10时干旱促进了WUE升高 ,其他时间无显著影响 ,表明光合参数对干旱的响应受日进程影响。     

Ecological biomass allocation strategies in plant species with different life forms in a cold desert,China

Biomass allocation patterns among plant species are related to their adaptive ecological strategies. Ephemeral, ephemeroid and annual plant life forms represent three typical growth strategies of plants that grow in autumn and early spring in the cold deserts of China. These plants play an important role in reducing wind velocity in the desert areas. However, despite numerous studies, the strategies of biomass allocation among plant species with these three life forms remain contentious. In this study, we conducted a preliminary quadrat study during 2014-2016 in the southern part of the Gurbantunggut Desert, China, to investigate the allocation patterns of above-ground biomass (AGB) and below-ground biomass (BGB) at the individual level in 17 ephemeral, 3 ephemeroid and 4 annual plant species. Since ephemeral plants can germinate in autumn, we also compared biomass allocation patterns between plants that germinated in autumn 2015 and spring 2016 for 4 common ephemeral species. The healthy mature individual plants of each species were sampled and the AGB, BGB, total biomass (TB), leaf mass ratio (LMR) and root/shoot ratio (R/S) were calculated for 201 sample quadrats in the study area. We also studied the relationships between AGB and BGB of plants with the three different life forms (ephemeral, ephemeroid and annual). The mean AGB values of ephemeral, ephemeroid and annual plants were 0.806, 3.759 and 1.546 g/plant, respectively, and the mean BGB values were 0.106, 4.996 and 0.166 g/plant, respectively. The mean R/S value was significantly higher in ephemeroid plants (1.675) than in ephemeral (0.154) and annual (0.147) plants. The mean LMR was the highest in annual plants, followed by ephemeroid plants and ephemeral plants, reflecting the fact that annual plants allocate more biomass to leaves, associated with their longer life span. Biomass of ephemeral plants that germinated in autumn was significantly higher than those of corresponding plants that germinated in spring in terms of AGB, BGB and TB. However, the R/S value was similar in plants that germinated in autumn and spring. The slope of regression relationship between AGB and BGB differed significantly among the three plant life forms. These results support different biomass allocation hypotheses. Specifically, at the individual level, the AGB and BGB partitioning supports the allometric hypothesis for ephemeroid and annual plants and the isometric hypothesis for ephemeral plants.     

流动沙漠腹地柽柳叶片的生长规律

研究极端干旱区植物叶片生长特征对筛选防风固沙植物有重要意义。在塔克拉玛干沙漠腹地塔中植物园柽柳专类园区,选择8种不同柽柳,研究了生长季内叶片生长规律。结果显示:1)柽柳属不同种的单叶表面积增长模型较为类似,总体差异不大,但其增长的规律性极强,以y=ax2+bx-c二项式增长模型为主;2)方差分析发现,不同柽柳种间单叶面积差异极显著(F>F0.01);单叶鲜重和干重的差异亦明显;3)柽柳单叶表面积与其干重存在正相关关系,以y=axb的乘幂关系为主。说明塔中沙漠植物园不同柽柳叶面积增长特征有所不同,防护林和固沙树种选择过程中可优先考虑叶面积增长迅速,生物量积累快的柽柳-长穗柽柳和刚毛柽柳。     

Distribution pattern of Caragana species under the influence of climate gradient in the Inner Mongolia region,China

There is a strong climate gradient in the Inner Mongolia region, China, with solar radiation and air temperature increasing but precipitation decreasing gradually from the northeast to the southwest. Sixteen Cara- gana species exist in the Inner Mongolia region. These Caragana species exhibit a distribution pattern across moisture zones and form a geographical replacement series. In order to examine the mechanisms responsible for Caragana species distribution pattern, we selected 12 Caragana species that exhibit a distinct distribution pattern across multiple moisture zones in the Inner Mongolia region, and determined the relationships between the leaf ecological and physiological traits of these Caragana species and the aridity index and solar radiation. Along with the climatic drought gradient and the solar radiation intensification from the northeast to the southwest, leaf eco- logical characteristics of Caragana species changed drastically, i.e. the leaf shape gradually turned from flat into tegular or tubbish; the leaf hair became denser, longer and lighter in color; the leaf area, leaf biomass and specific leaf area(SLA) decreased significantly; the leaf thickness and the ratio of leaf thickness to leaf area increased sig- nificantly; and the leaf chlorophyll content decreased significantly. As the climatic drought stress increased, osmotic potentials of the main osmotic adjustment substances and the cytoplasmic ion concentration of Caragana species increased significantly. Meanwhile, the total and free water contents and water potential of leaves decreased sig- nificantly; the ratio of bound to free water increased significantly; the stomatal conductance and transpiration rate reduced significantly; and the water use efficiency(WUE) increased significantly. In addition, with the intensification of climatic drought stress, peroxidase(POD) and superoxide dismutase(SOD) activities in leaves increased sig- nificantly. As a result, the malondialdehyde(MDA) content increased while the oxygen free radical content decreased. Our results showed that most of the leaf ecological and physiological traits of the 12 Caragana species varied in ac- cordance with the climatic drought gradient in the Inner Mongolia region, which reflected the adaptation of the Cara- gana species to the local climate conditions. With relatively more active metabolism and faster growth, the Caragana species in the northeast had strong competitive abilities; on the other hand, with stronger resistance to climatic drought stress, the Caragana species in the southwest could survive in harsh environments. Based on our results, we con- cluded that both the environmental gradients and the adaptive responses of Caragana species to their environments played important roles in the formation of the Caragana species distribution pattern across the Inner Mongolia region.     

黑果枸杞幼苗对干旱胁迫的生理响应

以黑果枸杞（Lycium ruthenicum Murr.）幼苗为试验材料,采用称重控水的方法,设置4个水分处理水平（对照CK、轻度胁迫D1、中度胁迫D2、重度胁迫D3）,研究黑果枸杞幼苗对不同程度干旱胁迫的生理生化响应。结果显示：① 随着干旱胁迫的加剧,黑果枸杞幼苗株高、基径生长量、生物量积累和叶重比在轻度胁迫下升高,中、重度胁迫下降低;根重比、根冠比在轻、中度胁迫下升高,重度胁迫下降低。② 随着胁迫时间的延长,各处理组脯氨酸（Pro）、可溶性糖含量逐渐升高,可溶性蛋白含量先降后升;丙二醛（MDA）含量先增后减再逐渐增加。③ 随胁迫时间的延长,各处理组超氧化物歧化酶（SOD）活性逐渐升高,过氧化物酶（POD）、过氧化氢酶（CAT）活性呈先升后降的趋势。综合分析认为,干旱胁迫下,黑果枸杞幼苗通过调整生长和生物量分配,减少地上部分水分散失,增加根系物质的积累,以吸收更多的水分,利用渗透调节物的积累来改变细胞渗透势,以便减少胞内水分流失。同时,通过提高保护酶活性来减弱膜脂过氧化对自身的伤害,表现出较强的耐旱性。     

The influence of cyst nematodes and drought on potato growth. 1. Effects on plant growth under semi-controlled conditions

Potatoes were grown under a permanent rain shelter in mobile containers in soil with or without potato cyst nematodes (Globodera pallida). The plants were subjected to an early drought stress period starting at planting until 43 days after planting, to a late drought stress period starting at 43 days until senescence at 92 days and to a drought control. Dry matter weight and characteristics of leaves, stems, stolons and roots were determined at periodic harvests. The early drought stress and nematode infection affected many plant organ characteristics in similar ways. Numbers of leaves, specific leaf area, plant height, specific stem weight, leaf area ratio, mean tuber weight and harvest index were reduced by both stress factors at early stages of growth.Later on, interactions between both stress factors which influence the development rate of the plants led to more diverse plant reactions. Plants of all treatments rapidly senesced at about 90 days after planting. Uninfected plants had then depleted the soil nutrient supply whereas the plants grown in the inoculated soil senesced as a result of the potato cyst nematode infection.