共查询到20条相似文献，搜索用时 31 毫秒
1.
AIM:The effects of Yigu capsule on tartrate resistant acid phosphatase (TRAP) secretion and apoptosis in rat osteoclasts were investigated in order to further explore its mechanism of preventing and treating osteoporosis.METHODS:(1) Twenty-month-old Sprague-daweley rats were randomly divided into two groups(Yigu capsule group and saline group), and the drug-containing serum and control serum were prepared. (2) The newborn Sprague-daweley rat osteoclasts were cultured with different concentrations of Yigu capsule drug-containing serum. At different time point, TRAP activity was measured and the survival osteoclast was counted under reverse microscope.The percentage of osteoclast apoptosis was observed under fluorescence microscope after acridine orange staining.RESULTS:TRAP activity was lower and the percentage of osteoclast apoptosis was higher in drug-containing serum group than in control group at 24, 48 and 72 h(P<0.01), respectively, and the survival osteoclasts were less in drug-containing serum group than in control groups at 24, 48 and 72 h(P<0.01).CONCLUSIONS:These data suggest that Yigu capsule drug-containing serum induces apoptosis and inhibits TRAP activity in osteoclasts, which may be one of the mechanisms of Yigu capsule preventing and treating osteoporosis. 相似文献
2.
AIM: To investigate effects of Retinervus luffae fructus (RLF) on level of serum lipid and body weight in hyperlipidemia rats. METHODS: Thirty-two male SD rats were randomly divided into four groups: control group (A), hyperlipidemia group (B), hyperlipidemia + RLF group (C), RLF group (D). Both group A and C were fed normal diet every day, while group B and group D fed high fat diet. Meanwhile, group C and D were administered with RLF solution at the dose of 10 mL/kg, respectively for 14 days, while group A and B were administered with drinking water. RESULTS: (1) At the end of experiment, a significant reduction was found in the levels of serum total cholesterol (TC) and triglyceride (TG) of group C animals treated with RLF solution; (2) The levels of serum TC of group D was progressively decreased compared to the level of serum TC at the beginning of experiment; (3) The level of high-density lipoprotein cholesterol (HDL-C) of group C remained unaltered 8d after treatment with RLF solution; (4) The body weight in group C was obviously lower than that in group B. CONCLUSION: RLF had an obvious hypolipidemic effect on hyperlipidemia rats. It can inhibit the decrease in the HDL-C and the increase of body weight in rats. 相似文献
3.
WANG Hua-dong YANG Jing LU Da-xiang QI Ren-bin WANG Yan-ping FU Yong-mei HUANG Qi-fu LI Chu-jie 《园艺学报》2004,20(2):219-223
AIM: To investigate the effect of siduqing decoction, a Chinese medicine, on survival rate and multiple organ dysfunction in mice challenged with LPS. METHODS: Mice were administered intragastrically with Siduqing decoction or distilled water (0.2 ml/10 g) twice a day for 3 days, two hours after Chinese herbal medicine treatment on day 3, LPS or normal saline was injected intraperitoneally, and survival rates in each group were recorded at 12-h intervals. In another experiment, mice were sacrificed at 12 h after LPS, lung, liver, kidney and small intestine were collected and processed for the H & E staining. In addition, Blood was collected at 10 h after LPS injection for determining alanine aminotransferase (ALT) activity, blood urea nitrogen (BUN) and creatinine (Cr) contents. RESULTS: At 96 h after LPS injection, the survival rate (27%, n=34) was lower in LPS group than Siduqing treatment group (65%, n=31, P<0.05). ALT activity, BUN and Cr contents in serum were higher in LPS group than control group, Siduqing treatment significantly attenuated a increase in ALT activity, BUN and Cr content in serum induced by LPS. Histological examination showed inflammatory injury in the lung and intestine, hemorrhage in the lung and kidney, degeneration, necrosis in the liver and kidney, while Siduqing treatment attenuated pathological changes induced by LPS. CONCLUSION: These data indicate that Siduqing has a protective effect against LPS-induced multiple organ injury and increases survival rate in mice challenged with LPS. 相似文献
4.
AIM: To observe the effects of some component of Chinese herbs for external use on proliferation of human umbilical vein endothelial cells (HUVEC) and investigate the mechanism of promoting tissue repair. METHODS: The method of MTT was used to examine the effects of Rg1, Rh1, perlolyrine, cinnamyl aldehyde, muscone, astragaluspolysaccharin (APS), velver antler polypeptide (VAP) and soluble extract of boswellia carterii birdw (BCB) on proliferation of HUVEC. RESULTS: APS did not promote proliferation of HUVEC at 9.75 mg/L-2.5 g/L; Rh1 promoted proliferation of HUVEC at 1.94 mg/L-0.5 g/L (P<0.05 or P＜0.01), and Rg1 inhibited proliferation of HUVEC at 31 mg/L (P<0.05); VAP promoted proliferation of HUVEC at 1 mg/L-0.5 g/L with optimal dose of 10 mg/L (P<0.01), Cinnamyl aldehyde promoted proliferation of HUVEC at 2 g/L(P＜0. 05); Muscone and soluble extract of BCB inhibited proliferation of HUVEC at 1 g/L, 0.5-2.5 kg/L(P＜0. 01), respectively; Perlolyrine inhibited proliferation of HUVEC at 0.125 g/L-0.5 g/L(P＜0. 01). CONCLUSION: The external herbs for supplementing Qi and warming Yang can promote HUVEC proliferation and improve angiogenesis during tissue repair. The external herbs for promoting blood circulation and accelerating capillary movement may have influence upon other stages of tissue repair. 相似文献
5.
GAO Zhi-jie WANG Liang-xing WANG Qun-ji XIE Yu-peng CHEN Yan-fan CHEN Shao-xian WANG Wei 《园艺学报》2000,16(5):436-439
AIM:To study the effect of ligustrazine on pulmonary hypertensive rats induced by hypoxic hypercapnia. METHODS:Thirty rats were randomly divided into three groups:control group(A),hypoxic hypercapnic group(B), hypoxic hypercapnia+ligustrazine(lig.) group(C). RESULTS: (1) Mean pulmonary arterial pressure(mPAP)of group B was significantly higher than that of group A and mPAP of group C was significantly lower than that of group B(P＜0.01),differences of mean carotid pressure(mCAP) were not significant among three groups (P＞0.05); (2)Electron microscopy and immunohistochemistry showed ligustrazine could inhibit the diposition of collagenous fiber(collagen typeⅠ)in pulmonary arterioles induced by hypoxic hypercapnia; (3) Plasma endothelin level of group C was significantly lower than that of group B (P＜0.01), serum (NO 2-/NO3-) of group C was significantly higher than that of group B (P＜0.01). CONCLUSION:Ligustrazine can inhibit pulmonary hypertension and the diposition of collagen type Ⅰ in pulmonary arterial wall induced by hypoxic hypercapnia. 相似文献
6.
LI Quan-feng WANG Xiao-ming ZHU Shi-jun AI Jing GAO Huan-huan XU Chang-qing 《园艺学报》2001,17(7):690-691
AIM:To examine the effect of compound salvae-dropping-pill (CSDP) on intracellular free calcium in cultured rat myocardial cells subjected to hypoxia and reoxygenation.METHODS:The Fluo- 3/AM was applied to probe intracellular calcium concentration and the fluorescent intensity was detected using laser confocal microscopy technique.RESULTS:Fluorescent intensity in hypoxia plus CSDP group was significantly lower (1 217.78±312.07) than that of hypoxia group (1 509±508.48), and the Fluorescent intensity of hypoxia/reoxygenation plus CSDP group was also markedly lower (1 567.91±577.61) than that of hypoxia/reoxygenation group (1 617.60±477.53).CONCLUSION:The cultured rat myocardial cells could be effectively protected by administration of CSDP in case of hypoxia and reoxygenation through decreasing the intracellular calcium concentration. 相似文献
7.
AIM: To study the effects of nuclear factor-kappa B decoy oligodeoxynucleotides on generation of nitric oxide and activated oxygen in lipopolysaccharide-induced mouse macrophages.METHODS: Mouse macrophage cell lines J774.1 cells were cultured with lipopolysaccharide and liposome-mediated oligodeoxynucleotides, and the level of nitric oxide, activated oxygen and inducible nitric oxide synthase were measured. RESULTS: Nuclear factor-kappa B \"decoy\"oligodeoxynucleotides decreased the activity of inducible nitric oxide synthase in lipopolysaccharide-induced macrophage, inhibited generation of nitric oxide and reactive oxygen species. The level of nitric oxide, activated oxygen and inducible nitric oxide synthase did not change in control group.CONCLUSION: Nuclear factor-kappa B \"decoy\"oligodeoxynucleotides decrease the generation of nitric oxide and activated oxygen in lipopolysaccharide-induced macrophages, which is probably due to inhibiting the binding sites of activated nuclear factor-kappa B specially. 相似文献
8.
CHEN Xiao-yin ZHANG Qun YANG Qin-he XIE Qiu-ling SHEN Qiang XU Yun-sheng 《园艺学报》2002,18(12):1529-1531
AIM: To examine the effect of traditional chinese medicine recipe, Taoren Honghua(semen persicae-flos carthami) decoction, on hyperlipidemia without symptom. METHODS: The plasma TC, TG, LDL, HDL, apolipoprotein(Apo) A, Apo Bof the patients with hyperlipidemia without symptom were measured using automatic analyzer (shimadiu CL-7200), the production of nitric oxide(NO) was detected by Greiss reaction, and SODactivity and MDAformation were examined using o-trihydroxy benzene and barbituric methotheds, respectively. RESULTS: After oral administration of Taoren Honghua decoction, the plasma levels of TC, TG, LDL, and MDAof the patients were markedly decreased, however, the plasma levels of ApoA, HDL, SODand NOwere significantly increased and almost no change was detected in the plasma level of Apo B. In control group, it was found that although the plasma level of TC, TG and LDL were decreased ( P<0.05 ) and ApoAas well as HDLwere increased, ApoB, SOD, MDA and NO production were all unchanged. CONCLUSION: The traditional chinese medicine recipe, Taoren Honghua decoction,has a significant therapeutic effect on patients with hyperlipidemia by removing blood stasis, promoting qi circulation and in turn reducing blood lipid level. 相似文献
9.
WANG Qian GONG Mu-xin ZHAO Hui WANG Yue-xiu YU Mei Li Ke-lin HUANG Qi-fu LI Bo-guang YAN Jing 《园艺学报》2003,19(5):664-667
AIM:To observe the changes of glycemia and serum cholesterol and triglyceride in diabetic nephropathy rats and therapeutic effects of Xiaoke Keli Ji.METHODS:3/4 nephrectomy was adopted firstly, three weeks later streptozotocin(STZ) was administered intraperitoneally to establish diabetic nephropathy model in rats. Animals were divided into four groups:model group, Xiaoke Keli Ji treatment group, positive control group and sham group. Changes of serum sugar and serum creatinine, cholesterol and triglyceride were examined at 1, 2, 3, 4, 5 weeks after STZ injection. Renal tissue samples were adopted at 6th week and studied by light microscopy.RESULTS:Model group demonstrated different degree of glomerular sclerosis. Lesions in treatment group were alleviated. Serum creatinine, serum sugar and serum cholesterol were higher at 1, 2, 3, 4, 5 weeks after STZ injection in model group than that of the sham group (P＜0. 05), serum triglyceride was higher at 1, 2, 3, 5 weeks after STZ injection in model group than that of the sham group(P＜0. 05). Xiaoke Keli Ji reduced those changes. Serum sugar was in positive correlation with serum lipoprotein.CONCLUSION:Diabetic nephropathy model was duplicated successfully. High serum sugar may lead to high serum lipoprotein, Xiaoke Keli Ji may treat diabetic nephropathy by reducing serum sugar and serum cholesterol and triglyceride. 相似文献
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AIM: To identify the enhancement of relatively specific immune responses by thymopeptide and 4 polysaccharides isolated from traditional Chinese medicine in the mice bearing cervical cancer. METHODS: The model of cervical cancer-bearing mice was established by implantation of cervical cancer U14 cells into the armpit of the right forelimb of the mice. Beginning from the 2nd day of tumor implantation, 4 polysaccharides Lycium barbarum polysaccharide (LBP), Angelica sinensis polysaccharide (ASP), Ganodema lucidum polysaccharide (GLP) and Panax ginseng polysaccharide (PGP)], thymopeptide or saline solution were intragastric administered for 21 days according to the experimental design. The animals were then killed, the tumor inhibitory rate and spleen index in treatment groups were compared with those in control group. The splenic T-lymphocytes (TLC) and natural killer (NK) cells, and the content of IFN-γ and IL-10 in the tumor tissues were also determined by flow cytometry and immunohistochemical methods. RESULTS: The order of tumor inhibitory rates was: ASP < PGP < thymopeptide < LBP < GLP. The effects of GLP, LBP and thymopeptide were statistically different from that of control group. No difference of tumor inhibition between PGP/ASP groups and control group was observed. The order of spleen index was: ASP < tumor control group < thymopeptide < GLP < PGP < LBP, that of CD4/CD8 enhancement was: PGP < ASP < GLP < thymopeptide < LBP, and that of CD49b+ NK hyperplasia was: ASP < thymopeptide < LBP < GLP < PGP,with statistically significant differences among the groups. The changes of IFN-γ and IL-10 in the tumor tissues showed that Th1/Th2 in thymopeptide group, LBP group and GLP group shifted to Th1. No shift to Th1 in PGP group and ASP group was observed. CONCLUSION: LBP, GLP and thymopeptide show significant inhibitory effects on tumor growth in U14 cervical tumor-bearing mice and can enhance the immune functions in the animals. 相似文献
12.
AIM:To investigate the inhibitory effect of Chinese medicine Jinan injection(JA) on Lewis lung cancer (LLC) in mice.METHODS:The C57BL／6J mice with Lewis lung cancer(LLC) were divided into normal saline(NS), Jinan high dose (JAH), Jinan middle dose (JAM), Jinan low dose (JAL) and cyclophosphamide(CTX) groups. The body weight changes and inhibitory rate of LLC in each group were observed. In addition, flow cytometry and TUNEL were used to detect the anticancer mechanism of Jinan.RESULTS:The body weights were increased significantly in JA-treated groups vs CTX and the resistant rate was 45.79%, 40.90%, 32.48% and 98.96%, respectively. The apoptotic rate was 24.19%, 14.95% and 13.93% in JAH, JAM and JAL, respectively, and the Jinan induced apoptosis of LIC in a dose-dependent manner.CONCLUSION:Jinan injection inhibites the growth of LLC, and the apoptosis induction may be one of mechanisms that Jinan treates LLC in mice. 相似文献
13.
AIM and METHODS:To study the effect of Nao-xue-bao at three different doses on blood coagulation,platelet aggregation by observing the changes in activated partial thromboplastin time (APTT), prothrombin time (PT), antithrombin-Ⅲ(AT-III), fibrinogen(Fng), plasminogen(Plg) and platelet aggregation(PAG). RESULTS:Compared with thrombosis group, the rats treated with Naoxuebao showed that the plasma APTT,PT were longer, and the activity of AT-III was increased. The content of Fng was reduced, TT was longer, there was a negative correlation between Fng and TT. Furthermore PAG-1, PAG-5 and PAG-M were reduced. CONCLUSION: Nao-xue-bao could inhibit thrombosis in different keys of blood coagulation. 相似文献
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AIM: To observe the effects of toddalia asiatica aqueous extract (Fei Long No 1, F01) on cardiac function and hemodynamics of acute myocardial ischemia in the animal model. METHODS: High positioned double-ligation of the anterior descending left coronary artery induced acute myocardial ischemia in New Zealand rabbits. F01 268 mg/kg were ip into the acute myocardial ischemic model, Cardiac function and hemodynamic measurements were performed before and after ligation and administration of F01. t-test paired was used for statistical analysis. RESULTS: After ligation all indices was reduced significantly except that LVEDP was markedly increased and t-dp/dt max had little change. After administration of F01 changes of most indices were reversed, and returned to or were close to the baseline. 1.5 h after administration of F01 action was more markedly. But the indices of left ventricle work and consumption of oxygen ( HR, TTI and TTI×HR) were reducing continuously. CONCLUSION: F01 markedly decreases ventricle work and consumption of oxygen of acute ischemic myocardium, so that the contractility, diastolic function of myocardium and cardial output are improved. These are the mechanism of protective effect on myocardial ischemia. 相似文献
15.
AIM: To elucidate the role of NO in the effect of tetramethylpyrazine (TMP) on vasomotion of arterioles induced by noradrenalin(NA).METHODS: Albino rabbits were anesthetized,respiration were maintained,the arterial pressure were measured with a pressure transducer attached to a polyethylene canula in the left common carotid artery,the intestinal loop was mounted on the stage of an inverted microscope and bathed in balanced solution.The inside diameter of the arterial was manually measured on a TV monitor using the TV camera mounted on the microscope.RESULTS: Topical applicated NA to measured field inducd spontaneous vasomotion which has frequency and amplitude.TMP inhibited vasomotion in vasodilation. Treatment with L-NMMA did not affect the effect of TMP.CONCLUSION: L-NMMA can not inhibit action of TMP on vasomotion,TMP may act on smooth muscle directly, not through endogenous NO. 相似文献
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AIM: To further elucidate the mechanism underlying the protective effect of Chinese herb Fu Sheng powder on vascular dementia. METHODS: Primary passage of neural cells of new born rats were subjected to the ischemia-reperfusion-like injury of hypoxia plus glucose deprivation followed by reoxygenation plus glucose, and the effect of \"Fu-Sheng powder\" on neural cells was examined. RESULTS: Both 5 hours of \"ischemia\" and 5 hours of \"ischemia\" plus 5 hours of \"reperfusion\" led to severe injury to neural cells, the formation of MDA and intracellular calcium concentration increased significantly, however, the activities of SOD and fluidity of neurons decreased significantly. It was also observed that Fu-Sheng powder could significantly alleviate this injury. CONCLUSION: Fu-Sheng powder had direct protective effect on neurons subjected to iscehmia-reperfusion-likeinjury, which might be one of the mechanisms underlying its therapeutic effect on vascular dementia. 相似文献
17.
AIM: To observe the expressions of HPS60 and HPS70 in hepatocytes in rats under treatment with zhiganning on steatohepatitis. METHODS: Male SD rats were randomly divided into large dose zhiganning group, small dose zhiganning group, ursodeoxycholic acid (UDCA) group, model group, normal control group. Except the normal control group, all the other rats were fed with high fat (88% standard diet, 10% lard, 2% cholesterol) and 35% alcohol 10 mL/kg twice a day. Prophylactic drugs were used at the same time. All rats were sacrificed at the 9th week. Routine histologic features of hepatic sections were observed by HE staining and penetrated electron microscope. The expressions of HSP60 and HSP70 in the liver were detected by immunohistochemistry, respectively. RESULTS: ⑴ The degree of steatohepatitis in the large dose zhiganning group and ursodeoxycholic acid (UDCA) group were significantly decreased compared with that in model group (P<0.05). ⑵ The expression of HSP70 in the large dose zhiganning group and ursodeoxycholic acid (UDCA) group were significantly higher than that in either model group or normal control group (P<0.05, P<0.01, respectively). The expression of HSP60 in the large dose zhiganning group was significantly higher than that in either model group or normal control group (P<0.05, P<0.01, respectively). ⑶ In the large dose zhiganning group and ursodeoxycholic acid group, ultramicroscopic structure of liver was nearly normal, which was significantly improved compared with model group. CONCLUSION: The results indicate that zhiganning and UDCA effectively prevente the steatohepatitis in rats induced by high fat diet and alcohol. The enhanced expressions of HSP60 and HSP70 may play an important role in the prevention of liver from injury. 相似文献
18.
AIM:To investigate the effect of berberine on IL-1 or tumour necrosis factor (TNF) induced polymorphonuclear leucocyte(PMN)-endothelium adhesion and adhesion molecules.METHODS:Based on the model of human umbilical vein endothelial cell (HUVEC), this study adopted Rose Bengal Stain, cell ELISA, immunocyto-chemical techniques to investigate the effect of berberine on PMN-endothelium adhesion and the expression of cell adhesion molecules (CAMs).RESULTS:Berberine inhibited IL-1, TNF-induced HUVEC adhesion for PMN when pretreated HUVEC and antagonised IL-1, TNF-induced upregulation of ICAM-1 on HUVEC. Meanwhile, TNF-stimulated PMN adhesion for HUVEC and CD18 upexpression on PMN was diminished in the presence of berberine.CONCLUSION: Inhibite PMN-endothelium adhesion by downregulating the CAMs expression to inhibite PMN migration across endothelium is one of the mechanisms of antiinflammation of berberine. 相似文献
19.
AIM: To evaluate efficiency of Chinese recipe of replenishing kidney and activating blood circulation (CRRKABC) to stave senile osteoporosis(OP) of male rats. METHODS: Forty male SD rats at age o12 months were randomly divided into 4 groups: ①24 months background control; ②27 months aging control; ③CRRKABC (low dose) and ④CRRKABC (high dose). General bone mineral density (GBMD), femur bending strength (FBS), serum-BGP and urine-Hyp/Cr were measured. RESULTS: GBMD and FBS in CRRKABC (H) group were significantly higher than those in 27 months aging control group, but lower than 24 months background control group (P<0.05); GBMD, FBS in CRRKABC groups were slightly higher than those in nylestriol's group (P>0.05). Furthermore, the level of serum-BGP in CRRKABC(H) group was higher and level of U-Hyp/Cr was lower than 27 months aging control group (P<0.05) but very close to that of 24 months background control group. The level of serum-BGP in CRRKABC (L) group was lower and level of U-Hyp/Cr was higher than 24 months background control group (P<0.05) but very close to that of 27 months background control group. CONCLUSION: CRRKABC could control osteoporosis in male rats induced by aging, in a dosedependant manner. 相似文献
20.
AIM:To study the protective effect of Ligustrazini(LGT) on gut barrier function after hemorrhagic shock-reperfusion. METHODS: Thirty white rabbits were divided randomly into 3 groups: control group (A),shock group (B) and LGT group (C). Malondialdehyde(MDA), tumor necrosis factor-α(TNFα), interleukin-1β(IL-1β) and nitric oxide products(NO2-/NO3-) contents were measured in intestinal mucosa at 3 hours following reperfusion,culture of bacteria in blood from rabbits of 3 groups was carried out,the intestinal mucosa was examined under optical and electron microscope. RESULTS: MDA, TNFα, IL-1β and NO2-/NO3- contents of intestinal mucosa remained unchanged in group C,but increased significantly in group B, compared with group A. Incidence of bacterial translocation in group B was markedly higher than that in group A at 30 min following reperfusion,there was not any difference between group A and group C. Under light and electronic microscope,in comparison with A and C groups,intestinal mucosa damage in B group became more severe. CONCLUSION: LGT can protect gut barrier from intestinal ischemia-reperfusion injury induced by hemorrhagic shock through reducing oxygen free radicals,raising nitric oxide and preventing inflammation. 相似文献