Release of arachidonic acid metabolites fromblood by cultivation of human amniotic fluid with oneself blood

AIM: To investigate the effect of human amniotic fluid on the release of thromboxane A₂ (TXA₂), prostaglandin I₂ (PGI₂) and Leukotriene C₄ (LTC₄) from blood cells. METHODS: 1 mL human amnioticfluid and 10 mL oneself blood collected from 38-41 weeks with cesarean section were cultured at 37 ℃ for 30 min, and then centrifuged. The supernatants were taken and stored at -70 ℃. TXB₂ and 6-Keto-PGF_1α of the superntants were determined by radioimmunoassay and LTC₄ by enzyme immunoassay. RESULTS: It was found that the levels of TXB₂ and LTC₄ in blood were elevated from (63.5±52.0)ng/L and (40.1±39.2) ng/L to (189.1±102.0) ng/L and (293.5±206.1) ng/L, respectively (P<0.01), after cultivation of oneself amniotic fluid with blood, the concentrations of TXB₂ and LTC₄ were higher in meconium-stained group than those in clear amniotic fluid group, but the concentration of PGI₂ only elevated slightly from (27.4±11.6) ng/L to (33.9±10.6) ng/L (P>0.05). CONCLUSION: Amniotic fluid might stimulate the release of TXA₂ and LTC₄ from blood, it might affect the balance of TXA₂ and PGI₂ in blood, which might play an important role in the pathogenesis of amniotic fluid embolism.     

利用远缘杂交创制抗虫长豇豆新种质

开展了长豇豆(Vigna unguiculata L. ssp. sesquipedalis)与其近缘种饭豆[V. umbellata(Thunb.)Ohwi&Ohashi]的远缘杂交,对亲本和杂交高世代(F₇)进行了田间抗虫水平和荚果性状观测鉴定。结果表明:(1)饭豆品种的田间抗虫水平普遍高于长豇豆品种;(2)在长豇豆和饭豆远缘杂交的全分离谱世代(F₆)363个株系中,初选了41个虫害发生较轻的单株,组成F₇代供后续鉴定;(3)田间试验观测到F₇代株系抗虫水平有较大分离,复选出7个高抗水平的重组株系;结合单荚质量、荚长和籽粒密度等荚果相关性状数据特征,决选出在营养生长期对斜纹夜蛾、甜菜夜蛾等主要害虫综合抗性达到高抗(HR)水平且荚果性状近似于长豇豆的株系2个,实现了长豇豆抗虫的种质创新。