共查询到20条相似文献,搜索用时 62 毫秒
1.
用病鸡的输卵管囊肿液中脱落的上皮细胞感染SPF鸡胚及BHK21、Vero、L929细胞,观察3~10 d鸡胚死亡情况及细胞生长情况,并进行病原分离、鉴定、检测等.结果为SPF鸡胚感染后盲传15代,1~11代基本无鸡胚死亡,12~15代鸡胚有死亡.细胞感染后第1代可见到CPE,敏感性L929>Vero>BHK21细胞,被感染的细胞及卵黄膜(第4代起)CF呈阳性;电镜观察到胞浆中有衣原体;Giemsa染色光镜下可见包涵体颗粒;碘染色及磺胺嘧啶敏感试验均呈阴性;病毒学及细菌学检测阴性;雏鸡感染试验阳性;病鸡肝、脾、输卵管等组织病理变化明显. 相似文献
2.
3.
4.
广东某肉鸽场幼龄鸽群发生以结膜炎、鼻炎和口腔炎,呼吸困难,腹泻,消瘦,生长发育缓慢为物征的传染病。从发病鸽分离到2株菌(SG1、SG2),经电镜形态学观察、人工培养基接种试验、碘胺敏感性试验及碘染色试验鉴定为鹦鹉热衣原体(Chlamydia psittaci)。用IHA试验检查该鸽群衣原体抗体,阳性率到达92.0%。本次的鸽子传染病诊断为鸽衣原体病。 相似文献
5.
鸡大肠杆菌病病原分离与鉴定 总被引:6,自引:0,他引:6
通过在甘肃及西北部分地区进行鸡大肠杆菌病流行病学调查,采集病料120份,分出大肠杆菌105株,分离率为85%。对26株大肠杆菌进行了血清型鉴定出血清型10个,其中血清型O78、O1、O2、O114占受检菌株的76.92%。 相似文献
6.
从山西太谷县的不同鸡群中采集鸡大肠杆菌病典型病料,经细菌学检验,分离出49株鸡大肠杆菌。动物试验结果表明:全部菌株对动物均具有很强的致病作用,多数菌株可使动物死亡。O因子血清鉴定发现15个血清型,分别为O78,O143,O15,O2,O18,O11,O8,O54,O119,O26,O14,O9,O1,O49,O5,其中O78型分离率为35/49,占总数的71。14%。 相似文献
7.
8.
9.
无菌采取某鸡场送检的疑似大肠杆菌病的病死鸡的心血、肝、脾、肾,进行病原菌的分离、鉴定、生化试验,动物试验和药敏试验。结果显示引起该鸡场鸡只发病的病原为大肠杆菌,并且该菌对小白鼠和鸡有较强的致病力,对阿米卡星、链霉素、庆大霉素、氟苯尼考敏感,对环丙沙星、痢特灵中度敏感;诺氟沙星不敏感;新诺明、红霉素、青霉素、氨苄西林、头孢唑啉钠则对其没有抑菌作用。表明该鸡场出现了耐药的致病性大肠杆菌。 相似文献
10.
鸡大肠杆菌病病原的分离鉴定 总被引:2,自引:0,他引:2
无菌采取某鸡场送检的疑似大肠杆菌病的病死鸡的心血、肝、脾、肾,进行病原菌的分离、鉴定、生化试验,动物试验和药敏试验。结果显示引起该鸡场鸡只发病的病原为大肠杆菌,并且该菌对小白鼠和鸡有较强的致病力,对阿米卡星、链霉素、庆大霉素、氟苯尼考敏感,对环丙沙星、痢特灵中度敏感,对诺氟沙星不敏感,新诺明、红霉素、青霉素、氨苄西林、头孢唑啉钠则对其没有抑菌作用。表明该鸡场出现了耐药的致病性大肠杆菌,在以后的肉鸡生产中应慎用抗生素,在使用药物治疗大肠杆菌病时,应根据药敏试验结果,选择敏感药物,且应注意交替用药,按疗程投药。 相似文献
11.
12.
13.
[Objective] The paper was to isolate and identify probiotics in the intestine of laying hens. [Method] The intestinal probiotics in laying hens at peak period were isolated using conventional separation methods; the physical and chemical properties of target strains and in vitro antibacterial effects were measured. Moreover, the safety test of chicks was conducted. [Result] Four strains of lactobacillus were isolated from the jejunum of laying hens, including Lactobacillus plantarum, Bacillus acidophilus, L. delbrueckii subsp. Delbrueckii and L. delbrueckii subsp. Lactis, and their inhibition zone diameters were 18.30, 16.07, 11.45, 17.26 mm, respectively. One strain of Lactobacillus, L. brevis, was isolated from the cecum, with the inhibition zone diameter of 10.26 mm. Three strains of bacillus were isolated from the cecum, including Bacillus subtilis, B. cereus and B. licheniformis, and their inhibition zone diameters were 9.25, 8.46 and 8.37 mm, respectively. Daily drinking 2 billion units of viable bacteria was the safe dosage for chicks. [Conclusion] Eight strains of probiotics had certain inhibitory effect on Escherichia coli O_2, and had no toxic side effects to chicks. Lactobacillus had strong antibacterial effect on E. coli O_2, while the antibacterial effect of bacillus was relatively weak. 相似文献
14.
试验旨在探讨稳定可靠的贮精腺上皮细胞分离及原代培养方法,为研究鸡贮精机理提供细胞模型。以鸡输卵管的子宫阴道交接部组织样为材料,采用酶消化法和组织块培养法分离培养母鸡贮精腺上皮细胞,观察母鸡贮精腺上皮细胞的培养情况,比较不同细胞培养方法获得贮精腺上皮细胞的生长情况。结果表明,用胶原酶或胰酶单独消化母鸡子宫阴道交接部组织,经100目过滤后获得的贮精腺上皮细胞24 h后可贴壁,但48~72 h后细胞死亡;用胶原酶Ⅺ(0.01 g/mL)与胰酶(0.25%)先后消化母鸡子宫阴道交接部组织后再经100目过滤获得的贮精腺上皮细胞贴壁性良好,24~48 h细胞出现明显增殖,72 h后细胞增殖速度减慢,开始死亡;用组织块培养法7 d可获得鸡贮精腺上皮原代细胞,该细胞可传2~3代;用组织块培养法获得的细胞进行免疫组化试验,发现细胞表达贮精腺差异表达基因编码的NXPH1蛋白,该蛋白在培养细胞内的表达符合其分泌蛋白特性,表明组织块培养法所获细胞可用于后续研究。综上,用组织块培养法获得的鸡贮精腺上皮细胞可为研究母鸡贮精腺机制提供细胞模型。 相似文献
15.
从陕西关中某鸡场分离到1株革兰氏阴性杆菌,经细菌学分离鉴定为鸡大肠杆菌,对小白鼠有极强的致病作用。药敏试验结果表明:该菌株对氯霉素、卡那霉素、丁胺卡那霉素等抗生素高度敏感,对青霉素、诺氟沙星、环丙沙星等抗生素不敏感。 相似文献
16.
LI Hao-xin DUAN Gang XIANG Xun ZU Fei YANG Zhi-yuan ZHU Qi DAI Fei-yan CHANG Hua 《中国畜牧兽医》2015,42(7):1883-1889
The assay was aimed to investigate the cause of death of diseased and dead piglets,we collected samples and identified by growth characteristics of bacteria in different mediums,biochemistry identification,PCR amplification of 16S rRNA genes and sequence analysis.The results showed that the isolate was Listeria.Drug sensitive test of the isolate to 20 antibiotics was detected.The results showed that this bacterium was highly sensitive to penicillin G,fosfomycin and amoxicillin and so on;However,it was resistant to furazolidone and so on.The growth curve of Listeria showed that the bacterium was in the proliferation period when 37 ℃ cultured 6 to 14 h.The results of this study provided scientific bases for detection and treatment of listeriosis in China. 相似文献
17.
18.
19.
20.
为了对某猪场病死仔猪的死亡病因进行调查,本试验采集病料进行研究,采用鉴别培养基分离、生化特性鉴定、PCR扩增细菌16S rRNA基因及测序分析等方法对样品中存在的细菌进行分离鉴定,结果显示分离菌为李斯特杆菌。采用标准K-B纸片法对分离菌株进行20种抗菌药物的药敏试验,并对其生长曲线进行测定。结果显示,此菌株对青霉素G、磷霉素、阿莫西林等药物高敏,而对呋喃唑酮等药物耐药。37 ℃培养6~14 h后细菌处于增殖期。本研究为中国仔猪李斯特杆菌病细菌学检测及治疗提供了科学依据。 相似文献