THE EFFECT OF HERBICIDES ON THE RELATION BETWEEN COVER INDEX AND YIELD INDEX

Summary. The effect of herbicide application on the relation between first and total number of contacts using the point quadrat method was studied. It was found that none of the herbicides tested — paraquat, dalapon, disodium methylarsonate, cacodylic acid and amitrole — affected the relationship appreciably, although differences in control were obtained. It is concluded that once the relation between first and total contacts is established in a particular field, the percentage weed control can he obtained with first contacts only, and the percentage weed control for total contacts calculated if necessary.
L'effet des herbicides sur la relation entre l'indice de couverture et le rendement     

DON毒素和小麦互作对小麦幼穗细胞可溶性蛋白的影响

 本研究经小麦幼穗细胞和DON毒素混合处理,研究抗赤霉病程度不同的苏麦3号(R)、望水白(R)、延冈坊主(R)、Alondra's (S)、安徽11(S)和仪宁小麦(S)的可溶性蛋白谱带的变化。用3μg/ml的脱氧雪腐镰刀菌烯酸(DON)与小麦幼穗细胞混和处理2、8、24、30h,分别提取可溶性蛋白,进行PAGE测定。结果显示,感病品种有多条谱带消失,如仪宁小麦、安徽11、Alondra's在处理2h后Rf 0.13的条带消失,处理8h后Rf 0.02条带消失,感病品种Alondra's和安徽11在处理24h后Rf 0.05的条带消失。抗病品种的电泳谱带没有变化,如苏麦3号、望水白在毒素处理后蛋白条带没有发生变化,抗病品种延冈坊主在处理24h后RfO.OS、O.11的蛋白带仅有变浅的趋势。研究还表明抗病品种苏麦3号、望水白、延冈坊主具有感病品种没有的Rf 0.3的蛋白带,且在毒素处理前后保持一致。     

化学除草剂对恶性杂草水葫芦的控制效果

水葫芦[Eichhornia crassipes(Martius)Solms-Laubach]分布于我国南方10余省市,给农业、水产养殖业、旅游业和周围环境带来了极大的危害。采用综合治理措施、发挥生物和化学防治的优势可以达到快速、有效、持续控制的目的。本文报道了4种除草剂对水葫芦的控制效果。     

THE EFFECT OF POST-EMERGENCE HERBICIDES ON THE VEGETATIVE AND REPRODUCTIVE GROWTH OF CENTROSEMA FUBESCENS BENTH. IN QUEENSLAND

Summary. 2,4-D-amine, 2,4-D ethyl ester, 2.4-DB-potassium. MCPA-sodium, diquat and a 1:1 mixture of 2,4,5-T butyl ester and 2,4-D ethyl ester were applied to the tropical pasture legume Centrosema pubescens Benth. (centro) grown either alone or with Panicum maximum Jacq. (guinea grass). Even at 0.5 Ib/ac (total) the 2,4,5-T/2,4-D mixture caused severe damage to seedling and mature centro. 2,4-D-amine, 2,4-D ethyl ester and 2,4-DB-potassium were less damaging and there were only slight differences in response to these. Diquat at 0.25 Ib/ac desiccated centro seedlings, but most plants survived and grew satisfactorily. There was some evidence that the growth of mature centro was depressed least when the herbicides were applied during vegetative quiescence.
Application just after the start of flowering altered the flowering pattern but did not reduce seed yields significantly. 2,4-D-amine at 0.75 and 1–5 lb/ac and 2,4-D ethyl ester at 1.5 Ib/ac decreased the percentage germination of abraded seed. Diquat 0.25 Ib/ac applied at the start of flowering caused significant increases in top growth and seed pod numbers, possibly the result of modification of the normal flowering pattern.     

THE EFFECT OF REPEATED FIELD APPLICATIONS OF FOUR HERBICIDES ON THE EVOLUTION OF CARBON DIOXIDE AND MINERALIZATION OF NITROGEN IN SOIL

The effect of repeated annual applications over 7-8 years of MCPA, triallate, simazine and linuron to field plots on the evolution of CO₂ and mineralization of nitrogen in soil samples incubated in the laboratory is described. The plots were either cropped and treated with standard doses, or uncropped and sprayed with doses 3-4 times above the level used in agricultural practice. While the applications of MCPA and tri-allate did not exert any inhibitory effects in soils from the uncropped plots those of simazine and linuron led to a lowering in CO₂ output in several instances and in mineral N on infrequent occasions. These effects are assumed to be the result of a difference in the content of easily-degradable organic matter between the treated plots and the controls. A direct anti-microbial action of the two herbicides is not very probable because in laboratory experiments with simazine up to 512 ppm the output of CO₂ and the mineralization of N was not affected while linuron at 500 ppm gave only a minor depression in CO₂ evolution. Effects on soil fertility are unknown but seem unlikely in view of the small extent and infrequency of the reductions observed. On the cropped plots the MCPA and tri-allate treatment showed no effects. With linuron and simazine a significant lowering in respiration and mineralization of N occurred on single occasions only, during a 5-year period.     

STUDIES ON THE EFFECTS OF HERBICIDES ON SOIL NITRIFICATION*

Summary. A perfusion technique was used to examine effects of selected herbicides (concentration range 50–1000 ppm) on the rate of oxidation of ammonia to nitrate in garden loam soil. Effects on the nitrification rate of soil, previously enriched with nitrifying organisms, were used to estimate direct action on the processes of ammonia oxidation. The kinetics of nitrification in fresh soil were used to estimate effects on bacterial growth and to detect possible adaptations to herbicide toxicity. The herbicides used could be ranged in the following order of increasing effectiveness as inhibitors of ammonia oxidation: dichlobenil < paraquat < picloram < 2,3,6-TBA = chlorthiamid < bromoxynil < chlorflurazole < ioxynil < propanil. Endothal only stimulated ammonia oxidation, even up to 1000 ppm. Endothal, paraquat and 2,3,6-TBA had no apparent effects on bacterial proliferation; the inhibitory effects of the remaining herbicides were in the order: picloram < dichlobenil = bromoxynil < ioxynil < chlorthiamid = chlorflurazole = propanil. With the exception of paraquat and endothal all herbicides seemed to become less toxic during perfusion of fresh soil, i.e. over the enrichment period of 26–28 days. In the case of propanil and chlorthiamid this could have been due to the destruction of the herbicide by soil micro-organisms. With picloram and 2,3,6-TBA it is probable that there was an adaptive loss of sensitivity of the nitrifying organisms to the toxic action of the herbicides. The evidence available suggests that Nitrosomonas is more sensitive than Nitrobacter to herbicide action. Recherches sur les effets des herbicides sur la nitrification Résumé. Une technique de perfusion a été utilisée pour examiner les effets d'une série d'herbicides (á des concentrations de 50 á 1000 ppm) sur le taux d'oxydation de l'ammoniac en nitrate dans un sol de jardin limoneux. Les effets sur le taux de nitrification du sol, préaiablement enrichi avec des organismea nitrifiants, ont été utilisés pour évaluer l'action directe sur les processus d'oxydation de l'ammoniac. Les données cinétiques de la nitrification dans un sol frais ont été utilisées pour évaluer les effets sur la croissance des bactéries et pour déceler les adaptations possible h la toxicité de l'herbicide. Les herbicides utilisés purent être classés comme suit dans i'ordre croissant d'activité inhibitrice de I'oxydation de I'ammoniac: dichlobénil<paraquat<piclorame<2,3,6- TBA = chlorthiamide<bromoxynil<chlorflurazole<ioxynii<propanil. Scul l'endothal provoqua une stimulation de I'oxydation de I'ammoniac jusquà 1000 ppm. L'endothal, le paraquat et le 2,3,6-TBA ne présentèrent pas d'activité apparente sur la proiifération bactérienne; les effets inhibiteurs des autres herbicides furent, dans I'ordrc: piclorame < dichlobenil = bromoxynil<ioxynil<chlorthiamide = chlorflurazole = propanil. A l'exception du paraquat et de l'endothal, tous les herbicides parurent devenir moins toxiques pendant la perfusion sur un sol frais, c'est-à-dire au deli delà période d'enrichissemenl de 26 à 28 jours. Dans le cas du dichlobénil et du chlorthiamide, ceci pourrait avoir été provoqué par la destruction de l'herbicide par lea micro-organismes. Avec le piclorame et le 2,3,6-TBA, il est probable qu'il y eut une adaptation consistanten une perte dela sensibilité des organismes nitrifiants vis-à-vis de i'action toxique des herbicides. Les résultats obtenus suggèrent que les Nitrosomonas sont plus sensibles que les JVitrobacter kà l'action des herbicides. Untersuchungen zum Einfluss von Herbiziden auf die Nitrifikation des Bodens Zusammenfassung. Mit Hilfe einer Perfusionsmethode wurde der Einfluss von bestimmten Herbiziden (Konzentration zwischen 50 und IOO ppm) auf die Oxydationsrate von Ammonium zu Nitrat in Garteniehmboden untersucht. Der Einfluss auf die Nitrifikationsrate des Bodens, der zuvor mit nitrifizierenden Organismen angereichert worden war, wurde als Mass fur die direkte Wirkung der Herbizide auf die Prozesse der Ammoniumoxydation verwendet. Die Kinetik der Nltrifikation in frischem Boden wurde als Mass für die Beeinflussung des Bakterienwaehstums und zur Feststellung einer moglicherweise auftretenden Adaptation an die Herbizidtoxizität verwendet. Für untersuehte Herbizide ergab sich, geordnet nach zunehnnender Hemmung der Ammoniumoxydation, folgende Reihenfolge: Diehlobenil<Paraquat<Pieloram<2,3,6-TBA = Chlorthiamid<Bromoxynil<Chlorflurazole<Ioxynil<Propanil. Endothal stimulierte die Ammoniumoxydation selbst in Konzentration bis zu 1000 ppm. Endothal, Paraquat und 2,3,6-TBA hatten keinen augenscheinlich Efifekt auf die bakterielle Proliferation; fur die Hemmwirkung der ubrigen Herbizide ergab sich folgende Reihenfolge: Picloram < Dichlobenil = Bromoxynil < Ioxynil < Chlorthiamid = Chlorflurazole = Propanil. Mit Ausnahme von Paraquat und Endothal wurden alle Herbizide wahrend der Perfusion dureh frisehen Boden, d.h. wahrend einer Enrichmentperiode von 26–28 Tagen, of Tensichtlich weniger toxiseh. Im Falle von Propanil und Chlorthiamid kann dies auf den Abbau der Herbizide durch die Bodenmikroorganismen zurüekzuführen sein. Bei Piclorani und 2,3,6-TBA handelte es sich wahrscheinlich um einen adaptiven Verlust der Empfindlichkeit der nitrifizierenden Organismen gegenuber der toxisehen Wirkung der Herbizide. Die Ergebnisse lassen den Sehluss zu, dass Nitrosomonas gegenüber Herbizide empfindlicher ist als Nitrobakter.     

三种磺酰脲类除草剂的光解和水解作用

磺酰脲类除草剂属高效除草剂,即用量少,除草活性高。其残留期因药剂而异,据报道,它们的主要降解方式是水解、微生物降解及水溶性光分解作用,在田间土壤含水量与药剂的降解速度成正比。作者研究了氯磺隆(Chlorsulfuron)、氯嘧磺隆(Chlorimuronethyl)、吡嘧磺隆(Pyrarosulfuron-ethyl)3种磺酰脲类除草剂水溶液的水溶性光分解作用和水解作用,对了解该类除草剂的降解作用有重要意义。     

二硝基苯胺除草剂对大豆生育影响的研究

作者于1981～1983年所作的系统田间与盆栽试验证明,二硝基苯胺类除草剂中的氟乐灵、二甲戊乐灵对大豆苗期生育有明显抑制作用,表现为:(1)出苗延迟,幼芽下胚轴肿胀、脆弱、矮化,子叶革质状,单位面积用药量对幼芽矮化的影响比混土深浅大;(2)侧根显著受抑制,这种抑制作用局限于药土层,混土深度对大豆侧根的抑制比用药量高低的影响更显著;(3)根瘤菌形成受抑制,根瘤数减少,叶面积减小。随着大豆生长,上述抑制作用逐步消失,对大豆产量无显著影响。在供试3种除草剂中,丁乐灵对大豆生育无影响。从安全、经济、高效考虑,氟乐灵与二甲戊乐灵的适宜用量为1.0公斤,丁乐灵为1.5公斤/公顷,将药剂施于大豆种子之上的土层为宜,亦即施药后耙地不宜过深,将药剂混拌于0～6厘米土层为佳。     

小麦丛矮病毒对灰飞虱雌、雄虫体繁殖力影响的初步观察

 灰飞虱是小麦丛矮病毒病的传毒介体。1969年yamada和Shikata报道过丛矮病毒可在灰飞虱虫体内繁殖。     

EFFECT OF TRANSPIRATION RATE OF BEAN PLANTS ON INHIBITION OF PHOTOSYNTHESIS BY SOME ROOT-APPLIED HERBICIDES

Summary. The inhibition of photosynthesis of bean plants (Phaseolus vulgaris L.) by root-applied herbicides was determined at different rates of transpiration, obtained by varying air humidity, light intensity and temperature. The plants were treated with 5×10^?6M of simazine, simeton, lenacil, isocil, diuron and fenuron in the nutrient solution. CO₂ uptake and transpiration of intact bean plants were measured continuously. The decrease in photosynthesis was more rapid at higher transpiration rates. For each herbicide the total transpiration to the 50% inhibition level of photosynthesis was almost constant at different rates of transpiration, indicating that the degree of inhibition is determined by the transpiration stream in the plants. Treatments with lenacil and diuron resulted in a lower degree of photosynthesis inhibition than did the other herbicides. The results are discussed in relation to uptake studies, herbicidal effects and adsorption phenomena of these and similar herbicides. They are also related to the field performance of such herbicides. Effet de la uitesse de la transpiration des haricots sur l'inhibition de la Photosynthèse par quelques herbicides administerés par les racines Résumé. L'inhibition de la Photosynthèse des haricots (Phaseolus vulgaris L.) par des herbicides administerés par les racines a été déterminèe à des taux différents de transpiration, obtenus en faisant varier l'humidité de I'air, l'intensité de la lumière et la température. Les plantes ont été traitées avec une concentration de 5 × 10^?6 M de simazine, de simétone, de lénacile, d'isocile, de diuron et de fenuron dans la solution nutritive. L'absorption du CO₂ et la transpiration des plantes intactes ont été mesurées en continu. La diminution de la Photosynthèse fut plus rapide pour un taux de transpiration élevé. Pour chaque herbicide, la transpiration totale jusqu'au niveau de 50% d'inhibition dc la Photosynthèse fut assez constante aux différents taux de transpiration, ce qui indique que le degré d'inhibition est déterminé par le courant de la transpiration dans les plantes. Le lénacile et le diuron ont moins influenceé la Photosynthèse que les autres herbicides. Les résultats ont été discutés par rapport à I'assimilation, aux effets phytotoxiques et aux phénomines de l'adsorption de ces herbicides et d'autres produits similaires, Les résultats ont été considerés aussi en relation avec l'effet de ces herbicides au champ. Einfluss der Transpìratìonsìntensität von Bohnen Pflanzen auf die Photosynthesekemmung nach Application einiger Herbizide über die Wurzel Zusammenfassung. Die Photosynthesekemmung von Bohnenpflanzen (Phaseolus vulgaris L.) durch einige üer die Wurzeln applizierte Herbizide wurde unter verschiedenen Transpirationsbedingungen, die durch Änderung der Luftfeuchtigkeit, Lichtintensität und Temperatur erhalten wurden, bestimmt. Die Pflanzen wurden mit 5 × 10^?6 M Simazin, Simeton, Lenacil, Isocil, Diuron und Fenuron in der Nährlösung behandelt. CO₂-Aufnahme und Transpiration intakter Pflanzen wurden kontinuierlich gemessen. Die Abnahme der Photosynthese war bei höherer Transpirationsintensität stärker. Für jedes Herbizid war die Gesamt transpiration bis zur Stufe der 50%-Hemmung bei verschiedenen Transpirationsintensitäten weitgehend konstant. Dieses Verhaiten zeigt, dass der Grad der Hemmung durch den Transpirationsstrom in der Pflanze bestimmt wird. Die Behandlung mit Lenacil und Diuron ergaben einen geringeren Hemmungsgrad als bei den übrigen Herbiziden. Die Ergebnisse werden im Hinblick auf Aufnahme, Herbizidwirkung und Adsorphánosphanomene dieser Herbizide besprochen. Sie werden auch auf die Wirkung dieser Herbizide im Freiland bezogen.     

栗瘿长尾小蜂生物学特性及寄生效能观察

栗瘿长尾小蜂(Torymus sp.)属长尾小蜂科,长尾小蜂属,是北京郊区栗瘿蜂幼虫的重要天敌。据调查,自然寄生率一般为50%左右,最高可达90%以上,且寄生效果逐年增高。对栗瘿蜂发生有显著的抑制作用。栗瘿长尾小蜂过去在国内未见报道资料。     

THE DEVELOPMENT OF HERBICIDES FOR POTATOES

Summary. Since 1961 the Weed Research Organization has been testing and developing herbicides for weed control in potatoes. Inthe three experiments which are reported, herbicides were compared with other forms of weed control. The first experimentin 1961 was exploratory and compared yields following hand-weeding versus no weed control versus several promising herbicides. The highest yield was produced by the hand-weeded crop. Several herbicide treatments applied before crop emergence gave useful selective weed control.
Assuming weed control to be necessary, selected herbicides were then compared in 1962 and 1963 with conventional mechanical cultivations. In both years successful weed control by herbicide resulted in a higher yield of potatoes than did mechanical cultivation although the soil-acting chemical used in 1962 caused some crop damage. The 1963 experiment included triangular planting patterns, at normal and high plant densities. Increases both in gross and marketable yield were obtained at normal plant density with the triangular arrangement as compared with the row crop. The high plant population resulted in the largest gross yield but a lower yield per plant and smaller average tuber size.     

大豆苗前除草剂多元混用的研究

本试验采用5因素、5水平的正交回归旋转设计的方法,于1983—1984年在哈尔滨、佳木斯和牡丹江地区的850农场作了5种大豆苗前土壤处理防草剂多元混用的研究。结果表明,一元(单用)时,异丙甲草酸、豆科威和茅毒的效果好,而Galex与Codal较差。多元混用时,以中等用量混用为好。在亩产超过100公斤的165个混用组合中,81.2％的组合出现在不用Galex的情况下,多数组合集中在茅毒、豆科威、异丙甲草胺和Codal的中等水平上。     

THE USE OF THREE SIMPLE,RAPID BIOASSAYS ON FORTY-TWO HERBICIDES*

Three rapid bioassays were tested on forty-two herbicides having several different modes of action. A 50% or greater inhibition of growth was found at 1 ppm with thirty-one herbicides in one or more of the bioassays. Of the remaining eleven herbicides, seven were detected at 10 ppm, two at 20 ppm and two at 30 ppm. The techniques used were a Chlorella bioassay, a root bioassay with sorghum, oat and cucumber and a shoot bioassay with sorghum and oat. The duration of the bioassays was 1, 2 and 4 days respectively. As a general rule, the Chlorella bioassay was especially sensitive to photosynthetic and respiratory inhibitors but not sensitive to herbicides with other modes of action, whereas the root and/or shoot bioassays were sensitive to most of the herbicides except the photosynthetic inhibitors. The use of the three bioassays simultaneously is suggested as a possible method for primary screening of herbicides.     

EFFECTS OF THE HERBICIDES TRIFLURALIN AND CARBETAMIDE ON NODULATION AND GROWTH OF LEGUME SEEDLINGS

Summary. The effects of pre-sowing incorporated applications of trifluralin and carbetamide on the growth and nodulation of Trifolium pratense L., T. repens L., T. dubium L. and Lotus pedunculatus Cav. were examined. At rates of 1·0 and 2·0 kg/ha a.i. of trifluralin and carbetamide respectively, nodulation and growth of all legumes was reduced. Root dry weight/plant and nodules/plant were positively correlated, suggesting that the herbicides affected plant growth but not the soil rhizobial population. There were several herbicide rate × legume species interactions.
Effets des herbicides trifluraline et carbétamide sur la formation de nodosités et la croissance des plantules de légumineuses     

春麦田除草剂的应用与杂草群落演替

作者采用定点定位试验,对春麦田除草剂应用与杂草群落演替趋势进行研究。结果表明:在杂草群落多样性麦田,小麦连作并分别连续5年施用同一种除草剂,原杂草群落中占优势的靶标杂草得以控制,而非靶标杂草和抗、耐药性杂草因失去竞争和制约对象而猖獗发展,发生量较原来增加几倍至几十倍,并形成优势种群,对小麦造成新的更严重的危害。一种除草剂在同田块连续施用4年,由于杂草群落演替,抗、耐药杂草兴起,除草效果显著下降而失去其使用意义。作者提出除草剂配套使用、轮用、混用等措施,并配合以合理的轮作制度,以减轻杂草群落长期受到单一的定向选择性压力。