Nematicides induced changes in the chemical constituents of potato tubers

Changes in the chemical constituents of potato tubers grown under different concentrations of 3 synthetic organic nematicides (carbofuran, aldicarb and phorate) and a natural plant product (sawdust) were investigated. There were no significant differences in the specific gravity, dry matter and starch content of tubers from different treatments. A significant increase in the content of non-reducing and total sugars was observed in all the treatments. Nematicidal treatments had significant effects on reducing sugars, true protein, free amino acids, orthodihydroxy phenols, beta-carotene and ascorbic acid. Application of nematicides reduced the content of total phenolic compounds which is a desirable change from a processing viewpoint. Potatoes grown under different nematicidal treatments can be processed into chips and French fries as they contain permissible levels of reducing sugars required for these forms of processing.     

Rate of Cooling Alters Chip Color,Sugar Contents,and Gene Expression Profiles in Stored Potato Tubers

When stored at temperatures below 10 °C, potatoes accumulate sucrose and the reducing sugars glucose and fructose. This process, cold-induced sweetening, has been studied extensively because potatoes with elevated reducing sugar contents produce undesirable, dark-colored products and acrylamide, a suspected carcinogen, during high-temperature cooking. Potatoes in commercial storages are cooled slowly, but many research studies have used potatoes cooled rapidly. In this study, effects of cooling rate and variety on chip color, sugars, and gene expression were examined. Sucrose and reducing sugar contents were substantially lower in slowly cooled than in rapidly cooled tubers of ‘Snowden’ and “MegaChip’ for the first 11 weeks after cooling to 3 °C began. Differences in gene expression for VInv, β-amylase, SPS, AGPase and GBSS were observed between cooling treatments and varieties. Overall, the data showed that cooling rate, time in storage, and variety influenced multiple aspects of cold-induced sweetening.     

Amylase Analysis in Potato Starch Degradation During Cold Storage and Sprouting

As the fourth most important food crop, potato plays a key role in food safety and economic development of the world. Harvested potato tubers can be stored for a long time, but sprouting and cold-induced sweetening (CIS) can seriously affect the quality of tubers during storage. One of the key pathways involved in CIS is starch degradation, in which both α-amylase and β-amylase play important roles. However, each amylase belongs to an extensive gene family and it is not clear which genes are the key regulators. In this study, we identified genes most likely regulating starch degradation. We first selected candidate genes from the public potato genome database and then investigated their expression patterns associated with reducing sugars and amylase activities. The results showed that the activity of α-amylase was mainly caused by StAmy23 and the activity of β-amylase was mainly caused by StBAM1 and StBAM7. In addition, α-amylase and β-amylase may play important roles in starch degradation of the tubers stored at low temperature and during sprouting, and the amylase activity may be regulated by the amylase inhibitor in cold-stored tubers.     

Possible involvement of fructose 1,6-bisphosphatase in cold-induced sweetening of potato tubers

Summary In measuring fructose 1,6-bisphosphatase (EC 3.1.3.11; FBPase) in potato tubers, we used anti PFP antibodies to ensure that the assay was specific for FBPase and that PPi:fructose 6-phosphate 1-phosphotransferase (EC 2.7.1.90: PFP) did not contribute to the production of fructose 6-phosphate (Fru-6-P). The involvement of cytosolic FBPase in cold sweetening was determined by monitoring enzyme activity during storage ofSolanum tuberosum cv. Erntestolz tubers at 2 or 8°C. In contrast to the rapid increase in sucrose and reducing sugars, the specific activity of FBPase showed no change and PFP rose slightly during storage at 2°C. Sugar concentrations and enzyme activities remained virtually unchanged during storage at 8°C. These data allow the interconversion of fructose 1.6-bisphosphate (Fru-1,6-P₂) to Fru-6-P and vice versa by FBPase and PFP, respectively, in potato tubers, but do not support a causal relationship between coarse metabolic control of FBPase and cold-induced sweetening.     

Predictive Markers for Cold-Induced Sweetening Resistance in Cold Stored Potatoes (<Emphasis Type="Italic">Solanum tuberosum</Emphasis> L.)

An approach has been developed to screen a large number of potato clones for cold induced sweetening (CIS) resistance in breeding programs. Two key enzymes responsible for reducing sugar accumulation during cold storage were identified. Clones with the A-II isozymes of UDP-glucose pyrophosphorylase coupled with low activity of vacuolar acid invertase enzyme had increased resistance to CIS by forming less suc, which is subsequently hydrolyzed to the undesirable reducing sugars, glc and fru. Six named cultivars and 192 genetically diverse clones from various breeding programs in USA were analyzed over two years for the two key enzymes and sugar concentration in cold stored tubers. The predictability for CIS resistance during cold storage was 94% both years. Clones classified as class A accumulated low concentration of reducing sugar glc during cold storage. It is suggested that these two predictor enzymes can be used for screening parents and selections in potato breeding program.     

Increase in phosphorylase activity during cold-induced sugar accumulation in potato tubers

Summary The accumulation of reducing sugars, sucrose and hexose phosphates in cv. Bintje and genotype KW77-2916 during storage at 2, 4, or 8°C was studied in relation to several catalytic activities. Bintje tubers accumulated sugars during storage at 2 or 4°C, whereas KW77-2916 showed reduced cold-sweetening at 2°C. The increase in glucose 6-phosphate and sucrose occurred concurrently and preceded the increase in reducing sugar concentration. Phosphorylase activity showed a strong interaction with temperature, storage duration and sugar accumulation in both genotypes. Invertase activity increased in Bintje concomitantly with the increase in reducing sugars, but this effect was less obvious in KW77-2916. The activities of other glycolytic and Krebs cycle enzymes showed no obvious correlation with sugar accumulation. It is suggested that the increase in phosphorylase activity acts as a triggering event in the sweetening of potato tubers during cold storage.     

Effect of storage period, cultivar and two growing locations on the processing quality of french fried potatoes

The source of raw potato tubers and the tubers’ duration of storage have a significant effect on color and texture of french fries. Controlling these variations is crucial to ensuring a consistent, high-quality end product. Russet Burbank and Shepody potatoes grown at two sites in Manitoba, Canada were stored for 9 and 11 months in 1994, and for 1 and 3 months in 1995, to determine the effects of short-term storage period, cultivar, and two tuber-growing locations on fry color and texture. French fry texture was measured by a puncture test, and peak force and peak deformation used as indices of textural quality. Large peak forces and deformations were assumed to be indicative of improved textural quality. Hunterlab L (lightness) value was used to determine fry color, higher values being associated with improved color quality. Fries processed from 11-month-old tubers had improved color and textural quality compared to those processed from 9-month-old tubers. Fries processed after 3 months tuber storage were darker in color but firmer in texture than fries processed from tubers after 1 month of storage. Differences in texture and color between the two cultivars were more evident with prolonged storage; fries processed from Russet Burbank potatoes were superior in texture and lighter in color than fries made from Shepody potatoes. With respect to site, fries processed from potatoes grown at Portage had better texture but darker color than those processed from Carberry potatoes. It was postulated that the calcium content of the soils may be responsible for both these results.     

Progress and Successes of the Specialty Crop Research Initiative on Acrylamide Reduction in Processed Potato Products

Acrylamide, a suspected human carcinogen, is a Maillard reaction product that forms when carbohydrate-rich foods are cooked at high temperatures. Processed potato products, including French fries and potato chips, make a substantial contribution to total dietary acrylamide. Health safety concerns raised by acrylamide in food increased financial risks to the potato industry and encouraged industry to take a proactive approach toward acrylamide mitigation. The USDA National Institutes of Food and Agriculture Specialty Crop Research Initiative (SCRI) on acrylamide reduction in potato was a cooperative endeavor in which industry partners worked at a systems level with university and government researchers to develop acrylamide mitigation strategies. Short-term goals focused on identifying advanced breeding clones and recently released varieties that have lower acrylamide-forming potential than standard potato varieties. Research was also directed at developing more efficient potato breeding methods, including marker-assisted breeding, genome wide selection, and improved phenotyping methods. Data from the National Fry Processing Trial (NFPT) and SCRI agronomic trial have shown that dramatic reductions in acrylamide are achievable through the use of new varieties that maintain low concentrations of tuber reducing sugars. Chipping potato trials coordinated by Potatoes USA and data from breeding populations suggest that maintaining low tuber reducing sugars through extended storage and lowering tuber asparagine content are options for decreasing acrylamide content in potato chips. To have an impact, new varieties must have exceptional agronomic performance and must produce finished products that meet requirements for consumer attributes including color, texture and taste. Data consistently show that this is more easily achievable in chipping potatoes than in fry processing potatoes.     

Influence of low fertility and vine killing on sugar development in apical and basal portions of Russet Burbank potatoes

Russet Burbank tubers from plants which died prematurely (low fertility plots) accumulated significantly greater amounts of reducing sugars in storage than tubers from plants which were adequately fertilized. Also, a significantly greater difference in relative amounts of reducing sugars between the apical and basal portions was obtained from tubers of the low fertilizer regime. The basal portion had a three times greater amount of sugars than the apical portion. This is of particular interest to processors of French fries who try to obtain uniformity of color in processed fries. The difference in total reducing sugar accumulation in storage between tubers of high and low fertilizer regimes was greater at the lower storage temperatures. Tubers allowed to mature in the ground (second harvest date) accumulated less sugars in storage than tubers dug shortly after vine kill.     

Screening for Chip-Processing Potato Line from Introgression of Wild Species’ Germplasms with Post-Harvest Storage and Chip Qualities

The processing of potatoes into chips is expanding in China. There is a need for new processing cultivars that are well-suited for the local ecological environment. Eleven potato varieties were bred from hybridization and backcrossing between the local cultivated potato varieties (Solanum tuberosum L.) and wild potato species. Lines from different wild species’ germplasms showed that the ability to accumulate reducing sugars was significantly different during low temperature storage (4 °C). A correlation analysis was conducted to determine the correlation coefficient among reducing sugars, acid invertase, free amino acids, chip colors and the content of acrylamide after storage at room and low temperatures. The lines 0706-116, 0737-6 and 0726-205 had low levels of reducing sugars, acrylamide content and acceptable chip colors for potato chip processing. The results indicated that the transfer of wild species’ processing traits into local cultivars by hybridization and continuous backcrossing is an effective potato breeding method and that the wild germplasm resources S. phureja and S. chacoense are suitable for improving the processing traits of local varieties.     

The effects of ozone and 1,8-cineole on sprouting,fry color and sugars of stored Russet Burbank potatoes

Ozone and 1,8-cineole were investigated as alternatives to isopropyl-n-(3-chloro-phenyl)carbamate (CIPC) to control sprout development of potato (Solatium tuberosum) tubers cv Russet Burbank during long-term storage. Sprout development was similar in ozone-treated and air-stored tubers, but both had much larger and more numerous sprouts than CIPC-treated tubers. Sucrose and reducing sugars in ozone-treated tubers were similar to levels in air-stored potatoes. Fry color was not different among potatoes stored in ozone, air and CIPC. Conversely, potatoes treated with 1,8-cineole did not produce any sprouts during the 25 week study. The fry color of tubers exposed to cineole was darker than either air-stored or CIPC-treated tubers. Sucrose and reducing sugar levels were higher in cineole-treated tubers than in tubers treated with CIPC.     

Growth and preharvest stress and processing quality of potatoes

The quality factors influenced by stress conditions are: total dry matter or starch content and distribution of starch, sugar content, types of sugars and distribution, texture, mealiness, flesh color, tuber size and shape, and tuber defects such as growth cracks, and hollow heart. Early stress during growth can cause tubers to become pointed on the stem-end with low starch content and high reducing sugars. Uneven growth due to stress results in growth cracks, or hollow heart and malformed tubers. Varieties differ considerably as to susceptibility. Stress generally causes lower starch content in tubers which results in greater sugar accumulation in storage. Relative maturity is also influenced by stress during growth. Immature and over-mature tubers (from prematurely dead plants) accumulate greater amounts of sugar in storage than tubers properly matured. Stress can also cause poor texture of processed products, such as tough texture of French fries as well as lack of mealiness. Flavor and color of processed products can also be detrimentally influenced by stress.     

Effect of Different Defoliation Systems of Ryegrass–Clover on Yield and Selected Quality Parameters of Organic Potatoes (Solanum tuberosum L.) for Industrial Processing at Harvest and After Storage

The nitrogen supply plays an important role in achieving quality characteristics in organic potato farming. Different defoliation systems of ryegrass–clover may influence the amount of fixed nitrogen available to the tubers. In a field experiment, the effect of different defoliation systems (cutting, mulching and a combination of them) of the pre-crop ryegrass–clover on selected quality attributes of organically grown potatoes, destined for processing into French fries (cv. Agria) or crisps (cv. Marlen), were conducted in two consecutive years (2003 and 2004). Parameters studied included compounds related to the sensory properties of potato food (tuber dry matter, starch, reducing sugars) as well as nutritional quality (nitrogen, minerals). Selected agronomic parameters such as total tuber yields and tuber size distribution were also compared. Furthermore, the influence of additional slurry fertilisation and 4 months of storage on these parameters were investigated. Total yields and portion of tuber yield 50–60 mm were significantly affected by the pure mulching variant in 2003. In 2003, the starch concentration at harvest, as well as after storage, was above the required minimum of 22% (cv. Marlen) and 19% (cv. Agria), while in 2004, this was slightly beneath these values. In 2004, a significant increase of starch concentration by the pure cutting variant was observed. In both years, mulched ryegrass–clover caused a decrease in tuber DM contents of 2.10 and 3.54%, respectively, compared to the cutting systems. Fertilisation significantly decreased DM and starch concentration. In 2004, the reducing sugar concentration of tubers of cv. Marlen from the pure cutting system was with 2.1 g kg^?1 FM above the required maximum of 1.5 g kg^?1 for crisps. In addition, storage led to a three to fivefold increase of reducing sugars concentrations in the tubers in this year. After slurry fertilisation, 8.8% higher N contents and up to 36% higher K concentrations were measured. Mulching of the pre-crop combined with slurry application led to an up to 61% higher tuber K concentration compared to the cutting system. The results indicate that different defoliation systems of the pre-crop ryegrass–clover and slurry fertilisation had only minor effects on internal tuber quality attributes. Quality parameters were more affected by the prevailing weather conditions in combination with the genotype of different potato cultivars.     

Investigations on the role of acid invertase and UDP-glucose pyrophosphorylase in potato clones with varying resistance to cold-induced sweetening

Fifteen potato (Solanum tuberosum L.) clones with varying resistance to cold-induced sweetening were analysed for vacuolar acid invertase (AcInv, EC 3.2.1.26) and UDP-Glc pyrophosphorylase (UGPase; EC 2.7.7.9) activities related to their ability to accumulate sugars following cold storage (4 C). The UGPase isozyme profiles for each clone were also determined. Immunoblot analysis demonstrated a 55 kD protein, in seven of the 15 clones, that reacted with UGPase antisera in addition to the 53 kD UGPase subunit previously reported. The UGPase activity of these clones was significantly lower than that of the “single subunit” clones. Basal AcInv activity showed a positive correlation to the Glc:Suc ratio across the clones that was moderately significant before and after cold temperature storage. The activity of UGPase, which limits the rate of Suc formation, was of secondary importance in limiting the rate of hexogenesis when vacuolar AcInv activity was excessive. It is suggested that with the potato clones from this breeding program that AcInv (and its inhibitor) plays a dominant role in the hexogenic pathway by regulating the hexose:Suc ratio. The finding of a new protein that is reactive with UGPase antisera may prove to play an important function in the regulation of Suc formation in potatoes.     

Role of invertase in the accumulation of sugars in cold-stored potatoes

Freshly harvested potatoes contain low levels of total invertase and high levels of invertase inhibitor. Total invertase increases sharply when potatoes are placed in cold storage, but the inhibitor is not depleted in all varieties. The absence of basal invertase activity in Norchip tubers indicates that an excess of invertase is not required for reducing sugar formation. Analyses of 37 varieties and seedlings after 3 months cold storage revealed that the reducing sugar content is not proportional to the invertase activity. High sugar contents are associated with low inhibitor levels, but low sugars are not necessarily associated with high inhibitor levels. The results indicate that invertase participates in reducing sugar formation, but other factors are responsible for the regulation of starch-sugar conversion in potatoes during cold storage.     

Effects of short periods of chilling and warming on potato sugar content and chipping quality

Two days of chilling (exposure to 1 C) had no detectable effect on chip color or sugar content of potato (Solanum tuberosum L.) tubers. Four days of chilling led to increases in sugars if potatoes were stored at 10 C or 19 C for 3 to 4 days after chilling ended. Longer chilling gave increases in sugars by the end of the chilling period, and returning tubers to 10 C or 19 C magnified the response. Glucose and fructose gave similar increases from chilling treatments, except that glucose levels were consistently greater. Sucrose was increased by chilling, but the response pattern did not always resemble that of the reducing sugars. Twenty-seven days of storage at 19 C after chilling lowered levels of reducing sugars late in the storage season but not in December. Cultivars differed in response to chilling. Kennebec tubers accumulated far more reducing sugars but much less sucrose than did Norchip tubers. Storage at 19 C after chilling lowered the sucrose content of Kennebec and Monona while raising that of Norchip.     

Irrigation and soil temperature effects on Russet Burbank quality

Potato growers in the Pacific Northwest suffer large economic losses in seasons with above normal temperatures, due to excess reducing sugars in tubers, which cause dark-end French fries. Our objective was to study irrigation management effects on potato quality, particularly the factors causing dark-end French fries or sugar-end syndrome. Solid-set sprinkler irrigated plots were established on potatoes at Kimberly, Idaho during the 1987, 1988 and 1989 irrigation seasons, and at Parma, Idaho in 1987 and 1988. Irrigation treatments were high and low frequency (3/week and I/week) and two or three water application amounts referenced to estimated evapotranspiration (ET, ET+20%, and ET-20%). Neither frequency nor amount significantly affected yields. Irrigation frequency had more influence on potato quality than application amount. More frequent irrigation produced slightly higher quality tubers and lower incidence of dark-ends when fried. Soil temperature was inversely related to tuber grade quality and directly related to percentage of sugar-end tubers. Soil temperature was about 0.5 C lower under the high frequency than under the low frequency irrigation regime. On these silt loam soils, allowing available soil water to decline to 50 percent had no adverse affect on yield or quality.     

Accumulation of sugars in microtubers of potato node cuttings (cv. Kennebec) during cold storage

Summary Microtubers of potato cultivar Kennebec were stored for two months at 2 or 8°C and sampled at selected intervals for the determination of glucose, fructose, sucrose and sprout weight. The aim of this experiment was to determine whether these microtubers show the same response to storage temperature with respect to sweetening as field grown tubers. During storage at 2 °C, reducing sugars and sucrose increased rapidly to a concentration of over 1.0 and 0.7 g/100 g fresh weight, respectively. In contrast, in tubers kept at 8°C, only a relatively slight increase in reducing sugar concentration to 0.3 g/100 g fresh weight was observed and sucrose concentration remained virtually the same.     

Sugar-end in Russet Burbank potatoes

Tissue selected from the stem- and bud-end of reconditioned tubers having sugar-end disorder was compared by color after frying and chemical composition to tissue removed from the two ends of tubers without sugar-end disorder taken directly from cold storage at 45 F, and after reconditioning 3 weeks at 70 F. Chemical analyses of selected tissue suggested that the dark finish-fry color at the stem-end of sugar-end tubers following reconditioning at 70 F was due to the inability of the physiological systems within the tissue to convert reducing sugars to starch and/or to use these sugars in the respiratory process. Of the components analyzed, only a high reducing sugar content was associated with a dark finish-fry color. Neither sucrose nor free amino acid content was associated with the sugar-end disorder. Extending the reconditioning period at 70 F for sugar-end tubers did not lower the reducing sugar content or the darkening of the stem-end tissue on frying.