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Abstract. An experimental Cryptobia salmositica infection in rainbow trout, Salmo gairdneri Richardson, produced suppression of the humoral response against sheep red blood cells as measured by direct haemagglutination. Two-month and 5-month infections produced equal suppression. The parasite also produced suppression of the humoral response against a bacterial pathogen, Yersinia ruckeri . Anti- Y. ritckeri titres were significantly lower in most fish infected with C. salmositica than in non-infected fish. Immunosuppression became evident when C. salmositica first appeared in the blood (first 2 weeks of infection), Immunosuppression was confirmed by challenge with Y. ruckeri . Mortality at challenge occurred in 64·3% to 83·3% of the fish already infected with C. salmositica at the time of initial Y. ruckeri exposure. There was no mortality at challenge if fish were not infected with C. salmositica at initial bacterial exposure, nor in those concurrently infected with both pathogens. Antigenic competition may have caused the immunosuppression. 相似文献
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Abstract. Rainbow trout that recovered from experimental Cryptobia salmositica infection 6 and 10 weeks earlier were protected against multiple intraperitoneal challenges of 50 000 and 10 000 parasites isolated from infected fish. The immunity was non-sterile; low parasitaemias were detected following a larger challenge (112 000 parasites). The indirect haemagglutination test was used to detect C. salmositica -specific agglutimns. Antibody titers increased during the first 18 weeks of infection. The infectivity of cultured C. salmositica was neutralized by incubation in heat-inactivated immune plasma. Infectivity of C, salmositica from infected fish was not neutralized by similar treatment. Complement fixing antibody was detected using the in vitro immune lysis test. Immune lysis occurred when cultured C. salmositica were used. Adoptive transfer of both leucocytes and plasma from immune fish conferred partial protection against the parasite in naive recipients. Complement fixing antibody may be important during early acute infection while phagocytosis may be important during the later chronic phase. 相似文献
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Abstract. Intraperitoneal implants of cortisol (cortisol suspended in hydrogenated coconut oil) were used to induce a graded hypercortisolism in rainbow trout, Salmo gairdneri Richardson. There was no obvious reduction in circulating lymphocytes in cortisol-implanted rainbow trout (70, 140 or 210μg/g body weight). Cortisol-implanted fish infected with Cryptobia salmositica had significantly higher parasitaemia and lower antibody litres compared with controls infected with haemonagellate but given coconut oil implants. These confirm the immunodepressive effects of the steroid. The parasite was also more readily detected at the early stage of the infection (shorter prepatent period, more infected fish and higher parasitaemia) in cortisol-implanted fish (140 and 210 μg/g body weight) than in controls. The mortality of the infected cortisol-implanted fish was higher than that of the infected fish implanted with only coconut oil, or the cortisol-implanted but non-infected fish. This in vivo study suggests that protective immunity against C. salmositica is, in part, due to a humoral response. 相似文献
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D. P. OLSON M. H. BELEAU R. A. BUSCH S. ROBERTS R. I. KRIEGER 《Journal of fish diseases》1985,8(1):103-111
Abstract. Strawberry disease is a subchronic, non–debilitating, and non–fatal inflammatory skin disease of rainbow trout that has been recognized for the past 25 years. Morbidity ranges up to 80% and the disease affects fishes from 15cm in length to brood stock size. Epizootiological data indicate that strawberry disease usually appears, first in the autumn and reaches peak incidence during the following winter and spring. Data also indicate that the disease is found only in certain trout farms in the western and Pacific north–western regions of the United States. Although the cause of strawberry disease is unknown, the normal clinical course of 8 weeks can be reduced by oral treatment with oxytetracycline. Clinical signs include appearance of bright red, raised, circumscribed, ulcerated and indurated lesions in the skin that usually range up to 3 cm or more in diameter. Microscopically, the disease is characterized as a subchronic, focal, non–suppurative dermatitis with ulceration and extensive infiltration of mononuclear inflammatory cells. 相似文献
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Abstract. Hetereogeneity of rainbow trout immunoglobulins was demonstrated by using monoclonal antibody 1A6 and polyacrylamide-gradient gel electrophoresis. Immunoglobulins defined by elisa using monoclonal antibody 1A6 were about 30% of the total immunoglobulins, detected by elisa using polyclonal antibodies, in healthy rainbow trout. In trout obtained from farms with a previous history of infectious viral diseases, 1A6-immunoglobulins were only about 14% of the total. Several serum pools from infected trout could be totally depleted of 1A6-immunoglobulins (about 12% of total immunoglobulins) by affinity chromatography over Sepharose immobilized monoclonal antibody 1A6. Polyacrylamide-gradient gel electrophoresis under denaturing conditions of total immunoglobulins, 1A6 immunoglobulins and no-1A6 immunoglobulins purified by affinity chromatography, showed a majority heavy chain of 70 KDa and a minority heavy chain of about 60 KDa, two light chains of 24 and 26 KDa, and a 11–14 KDa polypeptide. 相似文献
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Abstract. Two entirely separate outbreaks of PKD are described in an attempt to properly define the condition and enable it to be differentiated from other diseases of a similar nature. Losses of up to 75% of fingerling rainbow trout were recorded in one outbreak, illustrating the potentially serious nature of the disease. Although the kidneys showed the most obvious pathological changes, other organs were also affected. The host response was granulomatous in type, the foci of each cellular reaction being 5-20 μm eosinophilic cells considered to be parasitic. The cytoplasm of these cells contained numerous electron dense bodies some with a tail, but each with an electron lucent bar always orientated at right angles to the plasmalemma whenever they were near it. The most obvious cytoplasmic inclusion of these 'parasites' were other cells numbering up to five or six, each with its own nucleus and nucleolus, but devoid of any of the small electron dense bodies. The significance of either of these inclusions is unknown.
A limited differential diagnosis is given, and the argument presented that the condition PKD is the result of infection by a parasite, possibly an amoeba, in combination with poor quality water. 相似文献
A limited differential diagnosis is given, and the argument presented that the condition PKD is the result of infection by a parasite, possibly an amoeba, in combination with poor quality water. 相似文献
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Abstract. Electron microscopical observations of skin of rainbow trout revealed the presence of several types of phagocytic cells in the epidermis, most notably macrophages, some of which were seen crossing the basal lamina. Occasionally, filament-containing cells showed either melanin granules or phagosomes, suggesting these cells were also capable of phagocytosis. No cells with the characteristics of the Langerhans cells were observed, which does not necessarily preclude the possibility of macrophages being able to play an identical role as antigen-trapping cells. 相似文献
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M. J. PHILLIPS 《Aquaculture Research》1985,16(3):223-232
Abstract. Underwater television was used to observe the distribution and swimming behaviour of groups of farmed rainbow trout, Salmo gairdneri Richardson, in marine net cages. Undisturbed rainbow trout (mean total length 240 mm) aggregated near the surface, remaining motionless in slack water and formed polarized shoals at higher current velocities. Larger fish (mean total length 317 mm) occupied deeper parts of the cage and were more active during periods of slack water. The results are compared with previous work on farmed Atlantic salmon, Salmo salar L., and discussed in relation to the biology of wild rainbow trout and recent work on exercise, growth and condition in salmonids. 相似文献
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G. W. WARR B. R. GRIFFIN D. P. ANDERSON P. E. MCALLISTER B. LIDGERDING C. E. SMITH 《Journal of fish diseases》1984,7(1):73-82
Abstract. A rainbow trout with a malignant lymphoma of probable thymic origin showed direct spread of the tumour to the gills, metastases to the liver and head kidney, and the presence of abnormal circulating lymphocytes. The cells of the tumour possessed plasma membrane immunoglobulin detectable by immuno–fluorescence. Examination of the tumour cells by transmission electron microscopy revealed no evidence of virus associated with the cells. Infectious viruses could not be detected in the tumour tissue, and the tumour was not transmissible by injection of live tumour cells into young rainbow trout. Attempts to establish the tumour in long–term culture were unsuccessful. 相似文献
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Abstract The presence of lymphocytes in the epidermis of the rainbow trout, Salmo gairdneri Richardson, was confirmed by light and electron microscopical observation and by the use of an indirect immunocytochemical technique utilizing peroxidase-labelled antibody. These results are compared with those of other workers and the source and function of these cells in the epidermis of teleosts is discussed. 相似文献
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