双耳固体汤抗运动性疲劳功效研究

为研究双耳固体汤对小鼠抗运动性疲劳功效,随机将小鼠分为5组,低剂量组(12.5 mg·kg-1d-1)、中剂量组(25 mg·kg-1d-1)、高剂量组(50 mg·kg-1d-1)、空白对照组、阳性对照组.连续灌胃7 d,测定小鼠饲料消耗、体重变化、负重游泳时间,并检测肝脏指数、乳酸等生化指标.结果表明,与空白对照组...     

鸡腿菇复合制剂的抗S180肿瘤作用

旨在探讨鸡腿菇复合制剂的抗肿瘤作用,为鸡腿菇复合制剂的推广应用提供理论基础。通过腹股沟皮下注射S180肿瘤细胞建立小鼠肿瘤模型,把接种肿瘤细胞后的小鼠随机分为阴性对照组,阳性对照组,鸡腿菇复合制剂低、中、高剂量组,2周后检测小鼠体重和肿瘤大小的不同。结果表明抗癌药物环磷酰胺的肿瘤抑制率为71.8%;鸡腿菇复合制剂低剂量组(1 233 mg·kg-1)、中剂量组(2 466 mg·kg-1)和高剂量组(3 699 mg·kg-1)的肿瘤抑制率分别为14.3%、43.5%和50.4%。这说明在实验条件下,鸡腿菇复合制剂具有明显抑瘤作用,并呈现一定的剂量依赖性。     

杏鲍菇氨基酸增强小鼠抗疲劳能力研究

探讨杏鲍菇氨基酸对抗疲劳能力的增强作用.采用小鼠动物试验,将40只雌性昆明鼠分为空白对照组、低剂量组、中剂量组和高剂量组,分别灌胃生理盐水和20 mg·kg-1、60 mg·kg-1和180 mg·kg-1剂量的杏鲍菇氨基酸提取液.负重游泳试验中,高剂量组游泳时间为(31.88±1.01)min,与空白对照组的相比延长...     

我国栽培食用菌的重金属调查

测定双孢蘑菇、巴西蘑菇、茶树菇、黑木耳、鸡腿菇、金针菇、香菇、金顶侧耳、杏鲍菇、平菇、银耳11种食用菌的175个干样品中的砷(As)、镉(Cd)、汞(Hg)、铅(Pb)的含量.结果表明,11种食用菌干品中,砷含量范围为0.02 mg·k-1～0.78 mg·kg-1;镉含量范围为0.02 mg·k-1～1.949 mg·kg-1;汞含量范围为未检出～0.654 mg·kg-1;铅含量范围为0.040 mg·kg-1～3.217 mg·kg-1.我国栽培食用菌中镉、汞、铅含量比较安全,双孢蘑菇中汞含量高与黑木耳中铅含量高具有地域特性.     

不同农业土地利用方式对土壤重金属含量影响及生态风险评价

以撂荒地、水稻田和旱田为研究对象,分别测定其土壤As、Cd、Cu、Pb含量变化特征,结合单项污染指数法和潜在生态风险指数法,对研究区土壤进行生态风险评价,了解不同农业土地利用方式对土壤重金属污染状况及其生态风险,以期为农用地土地利用方式的选择提供参考依据.结果 表明:0～50 cm土层中,土壤As含量平均值表现为旱田(26.96士11.52)mg·kg-1＞水稻田(11.05±3.72)mg·kg-1＞撂荒地(9.04±6.87)mg·kg-1;土壤Cd含量平均值表现为旱田(0.64±0.03)mg·kg-1＞水稻田(0.44±0.18)mg·kg-1＞撂荒地(0.25±0.11)mg· kg-1;土壤Cu含量平均值表现为旱田(34.11土14.83)mg·kg-1＞水稻田(20.44土6.62)mg·kg-1＞撂荒地(13.90土6.64)mg·kg-1;土壤Pb含量平均值表现为水稻田(39.26±9.85)mg· kg-1＞旱田(38.87土7.10)mg·kg-1＞撂荒地(21.94土9.68)mg· kg-1.单项污染指数分析结果表明,4种重金属在水稻田和撂荒地中均无污染,而在旱田中As和Cd为轻度污染.潜在生态风险指数分析结果表明,4种重金属单因子潜在风险指数均属于轻微生态危害,Er值为Cd＞As＞Cu＞Pb,综合潜在生态风险指数RI均小于150,为轻微生态危害,其中以旱田土壤RI值最大,土壤重金属以Cd的贡献率最大.可见不同农业土地利用方式下,旱田对重金属的积累能力最强,水稻田次之,撂荒地最弱.农业活动虽一定程度上起到降低土壤pH的作用,但促进了土壤对重金属的积累.     

珠江三角洲菜园土硼和钼测土施肥技术指标研究

在不同地点、不同肥力水平的菜园土上进行代表性蔬菜微肥田间试验,对23个试验点的蔬菜相对产量与施硼、钼量,以及土壤有效硼、钼含量等试验数据进行相关统计分析,建立回归方程,以此建立菜园土有效硼和钼养分丰缺指标和各等级土壤的推荐施硼、钼指标:①蔬菜相对产量为50%、50%～75%、75%～90%、90%～95%、95%的各肥力等级菜园土壤有效硼丰缺指标分别为0.05mg·kg-1、0.05～0.20mg·kg-1、0.20～0.45mg·kg-1、0.45～0.65mg·kg-1、0.65mg·kg-1,各有效硼等级对应的施硼量分别为75g·(667m2)-1、50～75g·(667m2)-1、32～50g·(667m2)-1、26～32g·(667m2)-1、0～26g·(667m2)-1。②土壤有效钼丰缺指标分别为0.01mg·kg-1、0.01～0.07mg·kg-1、0.07～0.24mg·kg-1、0.24～0.35mg·kg-1、0.35mg·kg-1,各有效钼等级所对应的施钼量分别为12.5g·(667m2)-1、10.5～12.5g·(667m2)-1、9.5～10.5g·(667m2)-1、9.0～9.5g·(667m2)-1、0～9.0g·(667m2)-1。     

蒙古口蘑和干巴菌水提物对四氯化碳诱导小鼠急性肝损伤的保护作用

通过皮下注射四氯化碳橄榄油溶液建立小鼠急性肝损伤模型,分别灌胃蒙古口蘑(Tricholoma mongolicum)水提物(剂量分别为7、14、28 g·kg-1)和干巴菌(Thelephora ganbajun)水提物(剂量分别为8、16、24 g·kg-1),通过测定肝脏系数、血清中谷丙转氨酶(ALT)和乳酸脱氢酶...     

巴西菇麦角甾醇抗肿瘤活性及作用机理初探

采用小鼠S180移植瘤实验研究了巴西菇子实体中麦角甾醇的体内抗肿瘤活性,并通过巨噬细胞吞噬功能测定(中性红法)、脾淋巴细胞增殖功能检测(MTT法)、体外抑杀肿瘤细胞(MTT法)以及鸡胚绒毛尿囊膜(CAM)等试验初步探讨了麦角甾醇抗肿瘤活性的作用机理.结果表明,巴西菇子实体中提取的麦角甾醇在剂量10 mg·kg-1·d-...     

硒处理对薄壳山核桃果实品质及矿质元素积累的影响

【目的】探究硒处理对薄壳山核桃果实发育、矿质元素积累和果实品质的影响。【方法】以12 a(年)生盛果期薄壳山核桃‘威斯顿’嫁接株为试材,用0 mg·kg-1(CK)、20 mg·kg-1(T1)、40 mg·kg-1(T2)、80 mg·kg-1(T3)、160 mg·kg-1(T4)的亚硒酸钠溶液处理薄壳山核桃果实,采样测定薄壳山核桃果实各项生理指标。【结果】160 mg·kg-1硒处理后,薄壳山核桃的鲜果质量、果质量、仁质量分别为31.20 g、10.80 g、5.45 g,较对照组分别提高了13.91%、6.73%、11.22%。硒处理促进种仁中Se、Zn、Mn、Mg含量的积累,抑制Cu、Fe、K、Ca含量的积累。随着硒处理质量分数提高,种仁Se含量极显著提高,160 mg·kg-1硒处理后,Se含量(w,后同)为0.50 mg·kg-1,较对照极显著增加了49倍。硒处理与种仁Zn含量呈极显著正相关,160 mg·kg-1硒处理后,Zn含量较对照极显著提高了11.16%。硒处理对种仁Cu、K、Ca含量的抑制作用随着硒处理质量分数的增加表现出先增强后减弱的趋势,而硒处理对种仁Fe含量的抑制作用随处理质量分数增加逐渐减弱。各处理质量分数下,种仁中不饱和脂肪酸含量无显著差异,不饱和脂肪酸/饱和脂肪酸的比值大小依次为T4(11.40)T3(11.01)CK(10.87)T2(10.49)T1(10.27)。硒处理后,种仁中各氨基酸组分含量均高于对照,氨基酸总量大小依次为T4(12.21 g·100 g-1)T3(11.25 g·100 g-1) T1 (11.20 g·100 g-1)T2(11.09 g·100 g-1)CK(9.35 g·100 g-1),且160 mg·kg-1硒处理后的氨基酸含量(赖氨酸、蛋氨酸除外)显著高于对照组。【结论】160 mg·kg-1亚硒酸钠处理促进了薄壳山核桃果实的发育与生长,影响了果实各部分各元素的吸收,显著提高了薄壳山核桃种仁硒含量和各氨基酸组分含量。     

哈尔滨市东南郊菜地土壤重金属环境效应分析

通过分析哈尔滨市东南郊菜地土壤重金属含量及3种蔬菜作物对重金属的累积情况,研究土壤重金属的环境效应。结果表明,哈尔滨市东南郊土壤重金属含量分别为:Cu28.451mg·kg-1、Pb35.258mg·kg-1、Zn57.654mg·kg-1、Ni30.209mg·kg-1、Cr32.660mg·kg-1、Cd0.126mg·kg-1、As3.934mg·kg-1、Hg0.049mg·kg-1。在环境效应方面,作物根、茎、叶、果实重金属含量依次递减,即根部的重金属含量最高。不同蔬菜作物对土壤中重金属的富集能力不同,菜豆比较容易累积Pb、Cr,葱比较容易累积Cd,茄子则比较容易累积As。在食用安全性方面,这3种蔬菜的重金属含量均未超过国家标准。     

广叶绣球菌多糖对免疫低下小鼠海马组织cAMP-PKA-CREB-BDNF信号通路的影响

分别灌胃环磷酰胺所致免疫低下小鼠广叶绣球菌(Sparassis latifolia)多糖(SCPs,低、中、高剂量分别为100、200、400 mg·kg;),观察小鼠海马组织形态变化,检测5-羟色胺(5-hydroxytryptamine, 5-HT)、色氨酸羟化酶(tryptophan hydroxylase 2,TPH2)和环磷酸腺苷(cyclic adenosine monophosphate, cAMP)含量,测定细胞因子和cAMP-PKA-CREB-BDNF信号通路基因mRNA表达量、cAMP反应元件结合蛋白(cAMP response element binding protein, CREB)和磷酸化CREB(phosphorylated CREB, p-CREB)蛋白表达量,探讨SCPs对海马组织损伤的干预作用。结果表明:SCPs使免疫低下小鼠海马组织神经细胞数量增多,细胞间隙缩小,结构更为完整。与模型组相比,SCPs各剂量组5-HT和TPH2含量、中和高剂量组cAMP含量极显著增加;SCPs各剂量组TNF-α、中和高剂量组IL-1β mRNA表达量显著降低,SCPs中和高剂量组5-HT;受体、高剂量组蛋白激酶A和脑源性神经营养因子基因mRNA表达量显著增加;SCPs高剂量组CREB蛋白、各剂量组p-CREB蛋白表达量显著提高。研究结果为揭示广叶绣球菌多糖对海马组织保护作用的机制提供参考。     

Geniposide attenuates cognitive dysfunction in sleep-deprived rats by inhibiting TLR4/NF-κB signaling pathway

AIM To investigate the effects of geniposide （Gen） on Toll like receptor 4/nuclear factor-κB （TLR4/NF-κB） signaling pathway and cognitive dysfunction in sleep deprived rats. METHODS Wistar rats （n=120） were randomly divided into normal control （NC） group, model （M） group, low-dose （5 g·kg^-1·d^-1） Gen （Gen-L） group, medium-dose （10 g·kg^-1·d^-1） Gen （Gen-M） group, high-dose （20 g·kg^-1·d^-1） Gen （Gen-H） group and Gen-H+LPS （0.4 mg·kg^-1·d^-1, tail vein injection） group. After 7 days of intervention, the sleep deprivation model of rats in M group, Gen-L, Gen-M, Gen-H and Gen-H+LPS group was established by improved small platform water environment. The escape latency of Morris water maze experiment and the behavior correct rate of Y maze experiment were measured. The serum levels of S100B and neuron-specific enolase （NSE）, and the levels of interleukin-1β （IL-1β）, IL-6 and tumor necrosis factor-α （TNF-α） in hippocampus were detected by ELISA. The mRNA levels of TLR4 and NF-κB p65 were detected by RT-qPCR, and the protein levels of TLR4 and NF-κB p65 were determined by Western blot. RESULTS Compared with NC group, the escape latency, the serum levels of S100B and NSE, the hippocampal levels of IL-1β, IL-6 and TNF-α, and the mRNA and protein expression of TLR4 and NF-κB p65 were increased significantly in M group （P<0.01）, and the behavior correct rate was decreased significantly （P<0.01）. Compared with M group, the escape latency, the hippocampal levels of IL-1β, IL-6 and TNF-α, and the expression of TLR4 and NF-κB p65 at mRNA and protein levels were decreased significantly in Gen-L, Gen-M and Gen-H groups （P<0.01）, and the behavior correct rate was increased in turn （P<0.01）. Compared with Gen-H group, the escape latency, the serum levels of S100B and NSE, the hippocampal levels of IL-1β, IL-6 and TNF-α, and the expression of TLR4 and NF-κB p65 at mRNA and protein levels were increased significantly in Gen-H+LPS group （P<0.01）, and the behavior correct rate was decreased significantly （P<0.01）. CONCLUSION Geniposide may inhibit the TLR4/NF-κB p65 signaling pathway to effectively improve cognitive function in sleep-deprived rats and reduce hippocampus inflammation.     

Mechanism of Fuzilizhong decoction alleviating inflammatory damage of rats with non-alcoholic fatty liver disease

AIM To observe the effect of Fuzilizhong decoction on the inflammatory damage of non-alcoholic fatty liver disease （NAFLD） rats and to explore its mechanism. METHODS SPF male SD rats were randomly divided into 6 groups： control group, model group, high dose （20 mg·kg^-1·d^-1）, middle dose （10 mg·kg^-1·d^-1）, low dose （5 mg·kg^-1·d^-1） Fuzilizhong decoction group and Yishanfu （30 mg·kg^-1·d^-1）group, 8 rats in each group. A NAFLD rat modelwas established by intragastric administration of fat emulsion for 4 weeks. Then the drug was given for 4 weeks in each treatment group. HE staining was performed to observe the histopathological changes of the rat liver.The serum levels of interleukin-2（IL-2）, IL-6 and tumor necrosis factor-α（TNF-α） were measured by ELISA. The expression of toll like receptor 4（TLR4） and NF-κB p65 in liver tissues at mRNA and protein levels was determined by RT-qPCR and Western bolt,respectively. RESULTS Compared with control group, the inflammatory damage of liver tissue was more serious, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression TLR4 and NF-κB p65 in liver tissues were significantly increased in model group（P<0.05）. However, compared with model group, the liver pathological changes in each treatment group were significantly relieved, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression of TLR4 and NF-κB p65 in liver tissues were significantly reduced（P<0.05）.In addition, the changes of TLR4 and p-NF-κB p65 protein levels in liver tissue were consistent with the changes of TLR4 and NF-κB p65 mRNA. CONCLUSION Fuzilizhong decoction attenuates the inflammatory damages of NAFLD in rats by inhibiting TLR4/NF-κB p65 signaling pathway.     

Protective effect of gabexate mesilate on blood-brain barrier in rats with cerebral ischemia-reperfusion

AIM To investigate the protective effects of gabexate mesilate （GM） on blood-brain barrier （BBB） permeability in rat model with cerebral ischemia-reperfusion （I/R）. METHODS Adult male SD rats （n=180） were randomly divided into sham group, I/R group, nimodipine （NMP; 2 mg·kg^-1·d^-1） group and GM （5, 10 and 20 mg·kg^-1·d^-1） groups （n=30 in each group）. The rat model of cerebral I/R was established by blocking the middle cerebral artery with thread plug for 2 h. Ten min before modeling, the drugs were given intraperitoneally. The nerve function was detected by Longa scoring method. The permeability of BBB was measured by Evans blue permeation method, and the brain water content was measured by dry-wet weight method. The activity of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px）, and the content of malondialdehyde （MDA） in brain tissue were determined by biochemical analysis. The content of tumor necrosis factor-α （TNF-α）, interleukin-1β （IL-1β） and IL-6 was measured by ELISA. The mRNA expression of matrix metalloproteinase-2 （MMP-2）, MMP-9 and nuclear factor-κB （NF-κB） was detected by RT-PCR. The protein levels of MMP-2, MMP-9 and NF-κB were determined by Western blot. RESULTS Compared with I/R group, the Longa score, permeability of Evans blue and brain water content of the rats in GM （10 and 20 mg·kg^-1·d^-1） and NMP （2 mg·kg^-1·d^-1） groups were decreased. The activity of SOD and GSH-Px was increased, while the content of MDA was decreased. The content of TNF-α, IL-1β and IL-6 was decreased, and the mRNA and protein expression levels of MMP-2, MMP-9 and NF-κB were significantly down-regulated. Compared with NMP （2 mg·kg^-1·d^-1） group, the Longa score and permeability of Evans blue were decreased in GM （20 mg·kg^-1·d^-1） group, the activity of SOD was increased, and the content of MDA and TNF-α was decreased. The mRNA and protein expression levels of MMP-2, MMP-9 and NF-κB were down-regulated. All of the differences were significant （P<0.05 or P<0.01）. CONCLUSION GM has protective effect on BBB in the rats with cerebral I/R. Its mechanism may be related to inhibition of oxidative stress and inflammation, and down-regulation of MMP-2, MMP-9 and NF-κB expression.     

Propofol inhibits LPS-induced inflammatory response in microglia via TRPV1 ion channels

AIM:To investigate the effects of propofol (P) on the inflammatory response of microglia induced by lipopolysaccharide (LPS) and the mechanisms. METHODS:Mouse microglia BV2 cells were treated with LPS at 100 μg/L to establish a neuroinflammatory injury model. The BV2 cells were divided into 4 groups:control group (C group), model group (L group), L+P group and LPS+AMG517 group (L+A group). The level of tumor necrosis factor-α (TNF-α) in the cell culture supernatant was measured by ELISA. The mRNA expression of transient receptor potential cation channel subfamily V member 1 (TRPV1) was detected by real-time PCR. The protein levels of TRPV1, TNF-α, interleukin-1β (IL-1β), interleukin-6 (IL-6) and phosphorylated calcium/calmodulin-dependent protein kinase Ⅱ (p-CaMKⅡ) were determined by Western blot. The content of free Ca²⁺ in the microglia BV2 cells was detected by Fluo-3 AM assay. RESULTS:Compared with C group, the level of TNF-α was significantly increased in L group (P<0.01), but that in P group was not changed. Compared with L group, the level of TNF-α was significantly lower than that in L+P group within 4 h (P<0.01). Compared with C group, the mRNA expression of TRPV1 was significantly increased in L group (P<0.01). Compared with L group, the mRNA expression of TRPV1 was significantly down-regulated in L+P group (P<0.01).Compared with L group, the protein levels of TNF-α, IL-1β, IL-6 and p-CaMKⅡ and intracellular Ca²⁺ concentration were significantly lower than those in L+P group and L+A group (P<0.01). CONCLUSION:Propofol inhibits the inflammatory response of microglia by reducing the expression of TNF-α, IL-1 and IL-6, which may be related to the down-regulation of TRPV1 and p-CaMKⅡ and the reduction of intracellular Ca²⁺ concentration.     

Apocynin decreases cardiac cytokines in STZ-induced diabetic cardiomyopathy

AIM: This study was to investigate the effects of apocynin on the expression of IL-1β, IL-18, TNF-α and COX-2 mRNA in the diabetic cardiomyopathy heart.METHODS: Diabetes was induced in 8-week old C57 mice with one intraperitoneal injection of streptozotocin (STZ, 100 mg/kg). The experiments were divided into 4 groups: sham (n=6), apocynin control (Apo, n=6), diabetic cardiomyopathy (STZ, n=16) and apocynin treatment (STZ+Apo, n=16). At the end of 8 weeks after induction of diabetes, cardiac functions were evaluated by echocardiography. Cardiac tissue was analyzed for the expression of IL-1β, IL-18, TNF-α, COX-2 mRNA and phosphorylated Akt by real-time RT-PCR and Western blotting, respectively.RESULTS: The expression of IL-1β, IL-18, TNF-α and COX-2 mRNA increased in diabetic cardiomyopathy heart accompanied with cardiac dysfunction. Cardiac functions were improved with decreased levels of IL-1β, IL-18, TNF-α and COX-2 mRNA expression in apocynin-treated heart. Compared with reduction of phosphorylated Akt, apocynin significantly increased phosphorylation of Akt in diabetic cardiomyopathy heart.CONCLUSION: Apocynin decreases cardiac IL-1β, IL-18, TNF-α and COX-2 mRNA expression accompanied with improved cardiac function, which associates with the increase of phosphorylated Akt in diabetic cardiomyopathy heart.     

Effect of inhibiting nuclear factor-kappa B on TNF-α-induced expression of angiotensinogen and production of angiotensinⅡ in glomerular mesangial cells

AIM: To study the effect of inhibiting nuclear factor-kappa B (NF-κB) activity on the expression of angiotensinogen (AGT) and the production of angiotensinⅡ (AngⅡ) induced by tumor necrosis factor-α (TNF-α) in glomerular mesangial cells (MCs) of SD rats. METHODS: The MCs of SD rats were isolated and divided into three groups as follows: control; MCs treated with TNF-α, and the MCs treated with TNF-α + pyrrolidinedithiocarbamate (PDTC). The activity of nuclear factor-kappa B was measured by electrophoretic mobility shift assay. The expression of AGT was determined by RT-PCR for mRNA and Western blotting for protein. The concentration of angiotensinⅡ in supernatant was measured by RIA. RESULTS: The NF-κB activity in the MCs treated with TNF-α (20.67±9.14)×102 μg/cell was significantly higher than that in control cells ［(8.25±4.35)×102 μg/cell, P<0.01］ and the MCs treated with TNF-α+PDTC ［(7.20±4.57)×102 μg/cell, P<0.01］, and no significant difference was found between control and the MCs treated with TNF-α＋PDTC (P>0.05). The AGT mRNA level in the MCs treated with TNF-α (0.27±0.05) was higher than that in the control cells (0.20±0.05, P<0.05), and no significant difference was observed when compared with that in the MCs treated with TNF-α＋PDTC (0.22±0.06, P>0.05). The expression of AGT protein in the MCs treated with TNF-α (0.60±0.19) μg/cell was higher than that in the control ［(0.37±0.15)μg/cell, P＜0.05］ and the MCs treated with TNF-α＋PDTC ［(0.37±0.17)μg/cell, P<0.05］, and no significance was found between the MCs treated TNF-α＋PDTC and the control (P>0.05). The AngⅡ level in supernatant of cultured MCs treated with TNF-α ［(9.73±2.38)×10-5 ng·L-1/cell］ was significantly higher than that in the control ［(7.50±1.51)×10-5 ng·L-1/cell, P<0.05］ and in the MCs treated with TNF-α＋PDTC ［(6.94±1.46)×10-5 ng·L-1/cell, P<0.05］, however, the difference between the MCs treated with TNF-α＋PDTC and the control was of no significance (P>0.05). CONCLUSION: TNF-α activates the NF-κB in glomerular MCs, induces the AGT expression and the production of AngⅡ. Inhibition of NF-κB decreases the AGT expression and the production of AngⅡ. Therefore, the effects of TNF-α on AGT and AngⅡ may be mediated by NF-κB.     

Effects of diosmin on changes of TNF-α, IL-1β, IL-6, IL-8 and IL-10 in serum and kidney tissues of rats with kidney ischemia and reperfusion

AIM: To observe the effects of diosmin on the production of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), IL-6, IL-8 and IL-10 in serum and kidney tissues of rats with kidney ischemia and reperfusion (I/R). METHODS: Sprague-Dawley rats (180 in total) were randomly divided into 3 groups including sham operation group (sham),I/R group and diosmin+I/R group (diosmin+I/R). At the end of the experiment, the blood and kidney tissues were obtained and TNF-α, IL-1β, IL-6, IL-8 and IL-10 were detected by ELISA. RESULTS: The levels of TNF-α, IL-1β, IL-6, IL-8 and IL-10 in serum and kidney tissues in I/R group and diosmin+I/R group were significantly higher than those in sham group (P<0.01 or P<0.05). Following the development of the pathologic process, the level of TNF-α, IL-1β, IL-6 and IL-8 was significantly increased in I/R group and diosmin+I/R groups, but the level of IL-10 was significantly decreased in I/R group and significantly increased in diosmin+I/R group. The levels of TNF-α, IL-1β, IL-6 and IL-8 in I/R group was significantly higher than those in diosmin+I/R group (except TNF-α at 1 h in diosmin+I/R group). The level of IL-10 in diosmin+I/R group was significantly higher than that in I/R group (P<0.01 or P<0.05). CONCLUSION: Diosmin not only decreases the production of TNF-α, IL-1β, IL-6 and IL-8, but also promotes the production of anti-inflammatory cytokine IL-10, suggesting that the protective effect of diosmin on kidney I/R injury was associated with anti-inflammatory mechanism.