Fatty acid composition of broiler abdominal fat as influenced by temperature,diet, age and sex

1. Two environmentally controlled houses at 21°‐30°‐21°C (HT) and 21°C (LT) were used to study the effect of temperature on fatty acid composition of the abdominal fat of male and female broilers fed 13.8 and 13 MJ ME/kg diets and slaughtered at 34 and 54 d.

2. Extracts from the abdominal fat of 160 carcases and of the experimental foods were analysed for fatty acids by gas liquid chromatography.

3. Birds reared in HT had a significantly lower proportion of polyun‐saturated fatty acids (PUFA) in their abdominal fat between 34 and 54 d than birds in LT. The depot fat contents of oleic, linoleic and linolenic acids were all reduced by HT at 54 d.

4. For broilers slaughtered at 54 d, saturated fatty acid (SFA) content was much higher in females at high temperatures than in males while at low temperatures PUFA, especially linoleic, was much lower in males than in females.

5. Early finishing of broilers is advantageous from the viewpoint of fatty acid composition because the PUFA/SFA ratio declines significantly with age regardless of temperature.     

Corticosterone administration and dietary glucose supplementation enhance fat accumulation in broiler chickens

1. The effects of exogenous corticosterone administration and glucose supplementation on energy intake, lipid metabolism and fat deposition of broiler chickens were investigated. 2. A total of 144 three-d-old male chickens were randomly assigned to one of the following 4 treatments for 7 d: a low energy diet (10.9 MJ ME/kg, 200 g/kg CP) with or without corticosterone (30 mg/kg diet) and drinking water supplemented with glucose (80 g/l) or saccharine (2 g/l, control). 3. Body weight (BW) gain and breast and thigh muscle yields (% body mass) were all significantly decreased by corticosterone treatment. The relative cumulative feed intake (RCFI) and relative ME intake (RMEI), rather than the feed (FI) or ME intake (MEI) were increased by corticosterone administration. Both feed efficiency (FE) and caloric efficiency (CE) were decreased by corticosterone administration. Corticosterone administration had no obvious effect on water consumption. 4. Glucose supplementation had no influence on BW gain and breast and thigh muscle yield (as % of body mass). FI or RCFI was decreased while MEI or RMEI was increased by glucose supplementation. FE was improved by glucose treatment, whereas CE was reduced. 5. Liver weight and abdominal, cervical and thigh fat deposits were all significantly increased by either corticosterone or glucose treatment. 6. Plasma concentrations of glucose, urate, triglyceride, non-esterified fatty acids (NEFA), very low density lipoprotein and insulin were all significantly increased by corticosterone treatment. Glucose supplementation had no obvious influence on any of the measured plasma parameters except for NEFA, which were significantly increased. 7. Lipoprotein lipase activities in either cervical or abdominal adipose tissues, rather than in thigh fat tissue, were significantly elevated by either glucose or corticosterone treatment.     

Effects of dietary protein concentration and corticosterone injections on energy and nitrogen balances and fat deposition in broiler chicks

Reducing dietary protein concentration in isocaloric diets consistently decreased nitrogen and energy excretion, and increased dry matter (DM), non-protein DM (NPDM), energy retention and fatness. There were significant correlations, negative between dietary energy-to-protein (E:P) ratio and nitrogen excretion and positive between the E:P ratio and the retention of DM, NPDM and energy. Nitrogen excretion was correlated with energy excretion, and NPDM retention with energy retention. Corticosterone injections increased fatness despite significantly increasing nitrogen and energy excretion. A positive relationship was observed in corticosterone-treated birds between nitrogen excretion on the one hand and the retention of DM and NPDM on the other. Increased food intake because of a low dietary protein concentration was not evident when a sorghum-based diet was used. Moreover, corticosterone injections increased neither the food intake nor the fatness of chicks fed this diet. Decreasing the protein concentration in diets containing identical metabolisable energy (ME) levels slightly, but significantly increased apparent ME values in three out of four experiments. Corticosterone injection did not affect this variable.     

Effect of age,sex, and breed on serum cystatin C and creatinine concentrations in dogs

Veterinary Research Communications - This study investigated the effects of age, sex and breed on serum cystatin C (Cys-C) and creatinine in small breed dogs. This retrospective study included 250...     

Energy utilisation of carbohydrate, fat and protein sources in newly hatched broiler chicks

1. TME, TMEn and metabolisability (TME/gross energy) of energy-yielding foodstuffs were determined in 1, 3 and 10 d old broiler chicks, using the assay method developed by Murakami et al. (1994), in order to characterise energy utilisation during the 10 d after hatching. 2. TME, TMEn and metabolisability of dextrin and starch were low in chicks aged 1 d, and increased with age up to 10 d. Energy values of glucose and maltose at days 1 and 3 could not be determined because of the sudden death of birds soon after the feeding. TMEn and metabolisability of cereal sources were lower in d-old chicks than in those aged 3 and 10 d. At all ages, maize was better utilised than wheat and sorghum. 3. In the fat sources (coconut oil, beef tallow and safflower oil) no age dependency was observed in TME, TMEn and metabolisability. 4. Bioavailability of soyabean meal and fish meal was lower at 1 d than at 3 d and 10 d. Energy utilisation from casein was the highest among the protein sources tested and it was not age dependent. 5. It is concluded that energy utilisation of carbohydrate and protein sources during 10 d post-hatch tended to increase with age. Among the energy yielding foodstuffs fat sources seem to be better utilised, with no age dependency.     

Single injection of clenbuterol into newly hatched chicks decreases abdominal fat pad weight in growing broiler chickens

The aim of the current study was to examine the effects of clenbuterol injection into newly hatched chicks on both the abdominal fat pad tissue weight and the skeletal muscle weight during subsequent growth. Twenty‐seven 1‐day‐old chicks were divided into two groups, receiving either a single intraperitoneal (i.p.) injection of clenbuterol (0.1 mg/kg body weight) or phosphate‐buffered saline (PBS). Body weight gain, feed intake and feed conversion ratio were not affected by clenbuterol injection during the 5‐week experimental period, while the abdominal fat pad tissue weight of the clenbuterol‐injected chicks was lower than that of the control chicks at 5 weeks post‐injection. Plasma non‐esterified fatty acid concentrations were significantly increased in the clenbuterol‐injected chicks, while plasma triacylglycerol concentrations did not differ. Additionally, the enzymatic activity of fatty acid synthase was lower in the liver of the clenbuterol‐injected chicks. Conversely, the skeletal muscle weights were not affected by clenbuterol injection. These results suggest that a single clenbuterol injection into 1‐day‐old chicks decreases the abdominal fat pad tissue weight, but may not affect skeletal muscle weights during growth. © 2015 Japanese Society of Animal Science     

Effects of age, sex and breed on antipyrine disposition in calves

In domestic animals relatively little is known about the functions of hepatic microsomal enzymes and their role in biotransformation. In this study, antipyrine was used to assess microsomal oxidative function and particularly to determine the effect of age, sex and breed on drug metabolising enzymes. At birth, the elimination rate of antipyrine was very low as reflected by a half-life of 24 hours. The first two months of life were characterised by a steady decrease of antipyrine half-life values of three to four hours being reached at six months. The decrease observed during early life was not identical in the two breeds used in this experiment. By six months the Friesian calves eliminated antipyrine twice as fast as the Blue White Belgian (BWB) breed: 2.1 +/- 0.3 hours and 4.9 +/- 0.3 hours, respectively. The BWB breed is characterised by muscular hypertrophy and by a relative imbalance in muscle:body ratio. The apparent volume of distribution of antipyrine did not vary with age, sex and breed. No differences in antipyrine clearance were found between male and female calves.     

Antioxidant parameters of horses according to age,sex, breed and environment

Antioxidant defences interact to form an integrated system. There is no comprehensive and uniform view on issues concerning the antioxidant status in horses. Therefore, the aim of the present study was to estimate antioxidant parameters in horses of different age, sex and breed as well as environment and relationship between different antioxidants. Parameters of selected antioxidants including superoxide dismutase (SOD), glutathione peroxidase (GSH-px), total antioxidant status (TAS), ceruloplasmin (CP), bilirubin, uric acid, zinc, copper and selenium were determined in blood of 80 clinically healthy horses. Antioxidant parameters significantly varied between horses form different environments or different breed and sex. Age of horses had no significant effect on antioxidant parameters. Significant correlation coefficients were observed between enzymatic and nonenzimatic antioxidants. The nature of the relationship between the antioxidant system in horses with respect to environmental factors is rather complex and to date only a part of system is known.     

Effect of environmental temperature,dietary energy,age and sex on broiler carcase portions and palatability

1. Two environmentally‐controlled houses, one at a constant 21°C (low temperature), the other diurnally cycling at 21 to 30°C (high temperature) and two diets of 13.0 MJ ME/kg (low energy, LE) and 13.8 MJ ME/kg (high energy, HE) were used to study the effects of age and sex on broiler carcase portions, dissected tissue proportions, and meat quality.

2. Slaughtering at 54 d instead of 34 d produced broilers with breast and leg weights of 277 and 308 instead of 284 and 319 g/kg carcase respectively, and a higher cutting loss (31 versus 6 g/kg carcass).

3. Males had lower proportions of breast, breast meat and fat, but higher proportions of leg, leg bone, and total meat to fat ratio than females.

4. Cycling high temperature resulted in higher breast, and lower leg proportions than constant low temperature.

5. High dietary energy content increased cutting loss, proportion of breast fat and lowered meat to fat ratio when compared to low energy, but did not affect the flavour of the meat, which was improved by age and high environmental temperature.     

壳聚糖对黄羽肉鸡脂肪沉积的影响

选择健康49日龄黄羽肉用鸡（母）64只，随机分成4组。A为对照组饲喂基础日粮；B、C和D为试验组，分别在基础日粮中添加0．1％、0．3％和0．5％壳聚糖。饲养21d，研究日粮中添加壳聚糖对黄羽肉鸡脂肪沉积的影响。试验结果表明，在49日龄肉鸡日粮中添加0．1％、0．3％和0．5％壳聚糖能显著降低56、63和70日龄肉鸡的血清胆固醇（P〉0．05）和甘油三酯（P〉0．05）含量，降低70日龄肉鸡的腹脂率（P〉0．05），显著降低70日龄肉鸡的皮下脂肪，日粮中添加0．1％壳聚糖能达到较理想的降脂和降胆固醇的效果。     

Corticosterone administration and high-energy feed results in enhanced fat accumulation and insulin resistance in broiler chickens

1. Two experiments were conducted to investigate the effects of exogenous corticosterone administration (30 mg/kg diet) and dietary energy level on feed or energy intake and fat deposition in broiler chickens of 1 and 4 weeks of age. 2. Corticosterone treatment significantly suppressed body weight (BW) gain and reduced feed and caloric efficiencies. The retarded growth may conceal the stimulatory effect of corticosterone on feed consumption or metabolisable energy (ME) intake. A high-energy diet may increase energy intake and partially alleviate the suppressing effect of corticosterone on growth of broilers. 3. Corticosterone administration promoted the conservation of energy stores as fat at both abdominal and subcutaneous sites and this process occurred regardless of dietary energy level in ad libitum feeding status. A high-energy diet increased fat accumulation and showed no significant interaction with corticosterone treatment. 4. The suppressed development of breast and thigh muscles by corticosterone treatment was observed only in 1-week-old chickens fed on the low-energy diet. In contrast, the yield of breast muscle but not thigh muscle was significantly decreased by corticosterone in 4-week-old chickens, suggesting that the tissue specificity to corticosterone challenge is age dependent. 5. Plasma concentrations of glucose, insulin, triglyceride, non-esterified fatty acids (NEFA) and very low density lipoprotein were increased by corticosterone treatment regardless of diet treatment. A high-energy diet increased plasma levels of NEFA and resulted in hyperinsulinism in 4-week-old chickens but not in 1-week-old chickens. 6. Lipoprotein lipase (LPL) activities in adipose tissues may have been up-regulated by corticosterone treatment and showed tissue specificity. The increased LPL activities at ad libitum feeding status were not necessarily linked with the increased fat accumulation in corticosterone challenged chickens. 7. Corticosterone resulted in augmented energy consumption and altered energy redistribution toward lipid deposition. The induced insulin resistance and enhanced hepatic de novo lipogenesis by corticosterone are likely to be responsible for the increased fat deposition.     

Effect of early nutrition on fattening and growth of broiler chicks at 7 weeks of age

1. Six experiments were carried out with male and female broiler chicks in a factorial arrangement to evaluate the combined effects of fat concentration or energy-to-protein (E:P) ratio in diets fed from 1 to 7 or 14 d of age, and of the E:P ratio in diets fed from 1 or 2 to 7 weeks of age on fattening and performance of the birds. 2. Diets with a wide E:P ratio fed from 1 or 2 to 7 weeks consistently and significantly increased abdominal fat pad size at 7 weeks of age. On the other hand, in 5 of the experiments, the nutritional treatments from 1 to 7 or 14 d did not significantly affect this variable in chicks fed on diets with either the narrow or the wide E:P ratio. 3. Body weights and food utilisation at 7 or 14 d were consistently and significantly improved by increasing dietary fat concentration and using diets with a narrow E:P ratio. However, at 7 weeks of age, in only two of the experiments were body weights still significantly affected by early nutrition. 4. Diets with a wide E:P ratio consistently and significantly reduced the performance of chicks up to 4 weeks of age. Nevertheless, at 7 weeks of age the performance of these chicks tended to be better (at times significantly so) in most of the experiments, despite their excess fattening.     

Digestibility studies in broiler chickens: Influence of genotype,age, sex and method of determination

1. The influences of genotype, age and sex on droppings digestibility coefficients of a compound food were studied using male and female broiler chickens of three different genotypes at 2, 4 and 6 weeks of age.

2. Because the traditional method of determination of droppings digestibility coefficients of nitrogen may lead to systematic errors in estimating the feeding value of foodstuffs, a method is proposed to determine the ileal digestibility coefficients. The ileal method is compared with the droppings method for a mixed food and for two foodstuffs: wheat and solvent‐extracted soyabean meal.

3. Birds selected for efficient food conversion showed distinctly higher digestibility coefficients for all nutrients than birds selected for high growth potential or birds from a commercial strain.

4. The influence of age on digestibility coefficients was not consistent.

5. Female birds showed digestibility coefficients which were, in general, 3% higher than those of male chickens.

6. Interactions between genotype and sex and between genotype and age for energy metabolisability were the only interactions observed for digestibility measurements.

7. The method of determination influenced the amino acid digestibility coefficients of the mixed food and the relative feeding values of wheat and soyabean meal.

8. It is important to use well defined animals (genotype, sex, age) in evaluating foodstuffs.

9. The preferred method for determination of digestibility coefficients of nitrogen and amino acids is based on ileal sampling, although the differences in amino acid digestibility coefficients were small between methods.     

Fat accumulation,fatty acids and melting point changes in broiler chick abdominal fat as affected by time of dietary fat feeding and slaughter age

1. This work aims to quantify changes in fatty acid profile, melting point, abdominal fat accumulation and 2-thiobarbituric acid-reactive substances production depending on dietary fat source and age at slaughter, and to estimate the optimal date for the change from an unsaturated fat to a saturated fat diet or vice versa.

2. Treatments established were (1) birds fed 8% tallow from 21 to 49 d (TTT); (2) birds fed 8% tallow from 21 to 37 d and 8% sunflower oil from d 38 to 49 (TSS); (3) birds fed 8% sunflower oil from 21 to 37 d and 8% tallow from d 38 to 49 (STT); (4) birds fed 8% sunflower oil from 21 to 41 d and 8% tallow from d 42 to 49 (SST); (5) birds fed 8% sunflower oil from 21 to 49 d (SSS). Birds from each group were slaughtered on d 21, 29, 38, 40, 42, 44, 46 and 49.

3. The polyunsaturated fatty acids (PUFAs) proportion in the SSS group reached maximum values at d 40 and fitted a quadratic response. This group also showed a decrease in saturated fatty acids (SATs) and monounsaturated fatty acids (MUFAs) of lower intensity than the PUFA increase. The highest synthesis of SAT + MUFA was found in the SSS and TSS groups, whereas these had the lowest body-to-dietary PUFA ratio.

4. A high and quadratic increase in the MUFA proportion was observed during the first 10 d of feeding with the tallow-enriched diet at the expenses of the proportion of PUFA that quadratically decreased (minimum values at d 38).

5. Lipogenic and desaturation capacity decreased with age.

6. The TSS group increased tissue PUFA content faster that the SST group decreased PUFA content after the change in diet which indicates that the earlier feeding has to be taken into consideration for obtaining higher or lower changes in quality parameters.

7. The melting point of the SSS group showed a lower response to the dietary treatment in the initial period when compared to the TTT treatment.

8. The TTT, STT, SST and TSS groups showed similar fat accumulation, and changes in lipid oxidation were related to the day of dietary sunflower oil supplementation.

9. Based on the results, it would be possible to determine the most appropriate dietary programme and optimum slaughter age to obtain chicken meat with the desired quality characteristics.     

日粮中添加菊粉和不同脂肪类型对肉鸡行为、血清代谢物、肝脏脂肪、腹脂沉积以及组织中脂肪酸成分的影响

该试验旨在评价2种不同脂肪来源的饲料中添加菊粉对肉鸡行为、血清代谢物、肝脏脂质、腹脂、胸肌和腿肌中脂肪酸组成的影响。将240只1日龄雌性肉鸡随机分成6组,每组8个重复,每个重复5只肉鸡。试验采用3×2双因子试验设计,菊粉设3个水平（0、5和10g/kg）,日粮中2种类型的脂肪[棕榈油（PO）组和葵花籽油（SO）组]含量均为90g/kg。试验期为1～34d。日粮中的脂肪不影响肉鸡的体重增长,但PO组肉鸡的消化率低于SO组（p〈0.001）。日粮中含棕榈油增加了肉鸡腹脂沉积和血清中脂质及葡萄糖的含量。PO组肉鸡肝脏中的三酰甘油含量高于SO组。同样,日粮中的脂肪改变了肉鸡腹脂和肌内的脂肪酸组成,导致PO组肉鸡的C16：0和C18：1n-9含量高于SO组,C18：2n-6含量却更低。日粮中添加菊粉对肉鸡体重的增长（p=0.017）有二次效应,而对消化率没有影响。与对照组相比,菊粉减少了肉鸡肝脏油脂的总含量（p=0.003）、血清中甘油三酯含量以及低密度的脂蛋白胆固醇（31%）。菊粉添加到含葵花籽油的日粮中,增加了肉鸡腹脂和肌内多不饱和脂肪酸与饱和脂肪酸的比值。该研究结果表明：日粮中添加菊粉对肉鸡血脂的有益作用,是因为减少了三酰甘油含量。该结果同样证明,菊粉增强了葵花籽油提高肉鸡肌内多不饱和脂肪酸与饱和脂肪酸比值的能力。     

Effect of saturated fat on growth,body fat composition and carcase quality in chicks

1. The effects of feeding two fats with different degrees of saturation on skin tearing, carcase quality and body fat composition were examined in male and female chickens.

2. No differences in growth or efficiency of food utilisation were apparent on feeding the more saturated fat. Skin tearing was slightly reduced and carcase quality slightly improved on feeding the more saturated fat for 28 d.

3. Decreases in linoleic acid content in intra‐abdominal fat were apparent after 14 d feeding the diet containing saturated fat. These decreases were greater after feeding the saturated fat for 28 d.