Catalysis of Manganese（Ⅱ） on Chromium（Ⅵ） Reduction by Citrate

The catalysis of manganese（Ⅱ） （Mn^2＋） on chromium（Ⅵ） （Cr^6＋） reduction by citrate was studied through batch experiments with the concentration of citrate greatly in excess of Cr^6＋ at 25 ℃ and in pH ranges of 4.0 go 5.0. Results showed that at pH 4.5 within 22 h direct reduction of Cr^6＋ by citrate was not observed, bug for the same time when Mn^2＋ （50 to 200 μmol L^-1） was added, nearly all Cr^6＋ was reduced, with the higher initial Mn^2＋ concentration having faster Cr^6＋ reduction. In the initial stage of the reaction, the Cr^6＋ reduction could be described with a pseudo-first-order kinetics equation. In the lager stage of the reaction, plots of lnc（Cr^6＋） versus t, where c（Cr^6＋） is the Cr^6＋ concentration in the reaction and t is the reaction time, deviated from the initial linear trend. The deviations suggested that the pseudo-first-order kinetics did not apply go the whole experimental period and that some reaction intermediates could have greatly accelerated Cr^6＋ reduction by citrate. The catalysis of the intermediates increased with the reaction time and gradually reached stability. Then, the plot of lnc（Cr^6＋） versus t in the presence of Mn^2＋ was linear again, with the rate constant increasing by 102 times compared with the absence of Mn^2＋. Complexation between Mn^2＋ and citrate was likely a prerequisite for the catalysis of Mn^2＋ on the reaction. Additional experiments showed that introducing eghylenediaminegegraacegic acid （EDTA） into the reaction system strongly suppressed the catalysis of Mn^2＋.     

有机酸对污染土壤中镉释放的影响

There is limited information on the release behavior of heavy metals from natural soils by organic acids. Thus, cadmium release, due to two organic acids (tartrate and citrate) that are common in the rhizosphere, from soils polluted by metal smelters or tailings and soils artificially contaminated by adding Cd were analyzed. The presence of tartrate or citrate at a low concentration (≤ 6 mmol L^-1 for tartrate and ≤ 0.5 mmol L^-1 for citrate) inhibited Cd release, whereas the presence of organic acids in high concentrations (≥ 2 mmol L^-1 for citrate and ≥ 15 mmol L^-1 for tartrate) apparently promoted Cd release. Under the same conditions, the Cd release in naturally polluted soils was less than that of artificially contaminated soils. Additionally, as the initial pH rose from 2 to 8 in the presence of citrate, a sequential valley and then peak appeared in the Cd release curve, while in the presence of tartrate the Cd release steadily decreased. In addition, Cd release was clearly enhanced as the electrolyte concentration of KNO₃ or KCl increased in the presence of 2 mmol L^-1 tartrate. Moreover, a higher desorption of Cd was shown with the KCl electrolyte compared to KNO₃ for the same concentration levels. This implied that the bioavailability of heavy metals could be promoted with the addition of suitable types and concentrations of organic acids as well as reasonable field conditions.     

高岭石和氧化土悬液中铝的水解行为及有机酸对铝水解反应的影响

To evaluate the role of kaolinite and variable charge soils on the hydrolytic reaction of Al, the hydrolysis of Al ions in suspensions of a kaolinite and an Oxisol influenced by organic anions was investigated using changes of pH, Al adsorption, and desorption of pre-adsorbed Al. Kaolinite and the Oxisol promoted the hydrolytic reaction of Al above a certain initial Al concentration (0.1 mmol L^-1 for kaolinite and 0.3 mmol L^-1 for the Oxisol). The Al hydrolysis accelerated by kaolinite and the Oxisol increased with an increase in initial concentration of Al and was observed in the range of pH from 3.7 to 4.7 for kaolinite and 3.9 to 4.9 for the Oxisol. The acceleration of Al hydrolysis also increased with the increase of solution pH, reached a maximum value at pH 4.5, and then decreased sharply. Al hydrolysis was promoted mainly through selective adsorption for hydroxy-Al. Soil free iron oxides compensated a portion of the soil negative charge or masked some soil surface negative sites leading to a decrease in Al adsorption, which retarded acceleration to some extent. For the Oxisol organic anions increased the proportion of adsorbed Al³⁺ in total adsorbed Al with the increase in soil negative surface charge and eliminated or reduced the acceleration of Al hydrolysis. Different organic anions inhibited the hydrolysis of Al in the order: citrate > oxalate > acetate (under initial pH of 4.5). The formation of Al-organic complexes in solution also inhibited the hydrolysis of Al.     

初始浓度对六氯苯在土壤中的吸附-解吸的影响及解吸

Adsorption and desorption are important processes that influence the transport, transformation, and bioavailability of hexachlorobenzene (HCB) in soils. To examine the adsorption-desorption characteristics of HCB, equilibrium batch experiments were carried out using two soils (red soil and paddy soil) with different initial HCB concentrations (0.25, 0.50, 0.75, 1.00, 1.50, 2.50, 3.50, and 5.00 mg L^-1) by using 0.01 mol L^-1 calcium chloride as the background solution. The successive desorption experiments (48, 96, 144, 192, and 240 h) were conducted after each adsorption equilibrium experiment. The results revealed that adsorption and desorption isotherms of HCB on two soils were nonlinear, which can be best described by the Freundlich equation with the square of the correlation coefficient (r²) ranging from 0.97 to 0.99. Desorption of HCB from the two soils exhibited hysteresis at all HCB concentrations because the Freundlich desorption coefficients were always higher than the Freundlich adsorption coefficients. The hysteretic effect was enhanced with increasing initial HCB concentration, and positive hysteresis was observed at different concentrations.     

硒酸根与氢氧根离子在可变电荷土壤表面的交换反应: Ⅱ. 氢氧根离子释放的动力学

A self-made constant pH automated titration instrument was used to study the kinetics of hydroxyl release during selenite reacting with variable charge soils. The rate of hydroxyl release was very rapid at the first several minutes, then gradually slowed down, and at last did not change any more. The experimental data was well fitted by the Langmuir kinetic equation, and with increasing selenite concentration or decreasing solution pH, the reaction lasted longer, the maximum of hydroxyl release (x_m) increased, and the binding constant (k) decreased. The time of hydroxyl release with Xuwen latosol was much longer than that with Jinxian red soil.     

Relationship between granitic soil particle-size distribution and shrinkage properties based on multifractal method

The mechanical properties of granitic residual soils vary with depth due to changes in soil type and heterogeneity caused by weathering. The purpose of this study was to relate the spatial variation of particle-size distribution (PSD) of granitic soils with soil shrinkage parameters using multifractal theory. The heterogeneity of PSD and pedogenic processes were depicted in detail by multifractal dimensions. The PSD generally increased with the increase of profile depth in accordance with the variation of single fractal dimension (D) ranging from 2.45 to 2.65. The shrinkage limit was greatly influenced by the multifractal dimension parameters, including information dimension (D₁) and capacity dimension (D₀) (Adjusted R²=0.998, P < 0.01), and the maximum linear extensibility (κ_v) was determined by spectral width (△α) and bulk density, with the latter explaining 89% of the total variance of κ_v (P < 0.01). Soil shrinkage characteristic curve was fitted by the modified logistic model (R² > 0.97, root sum of squares < 0.1), and the water variation corresponding to the maximum change rate of linear extensibility was determined by the silt content (R²=0.81, P < 0.01). Overall, the shrinkage of granitic soils was primarily influenced by PSD and soil compactness.     

不同N水平紫色土上不同水稻品种吸收N、K的动力学

A pot experiment was conducted to study the effect of nitrogen fertilizer on nitrogen and potassium uptake by four rice cultivars. Results showed that the quadratic parabola relationship between biomass of rice and nitrogen levels was observed, with the maximum biomass at the nitrogen level of 150 mg kg^-1. The rates of nitrogen and potassium uptake by the four rice cultivars depended on growth stage and rice cultivar with the maximum rate of N in Shanyou-63 and maximum rate of K in Kaiyou-5 (hybrid rice), respectively. The kinetics of nitrogen and potasssium uptake by rice plant could be quantitatively described by the following equations: y = a + blogt, y = ab + t^1/2 and y = ae^-bt. The b value in the equations was correlated significantly to the rates of nitrogen and potassium uptake (N_R and K_R, r = 0.901^**~0.990^**), suggesting that the b value could be used to distinguish the index of nitrogen and potassium uptake capacity of rice. The maximum values of nitrogen uptake by plant (b value) and apparent recovery of fertilizer nitrogen were observed in Shanyou-63, and the minimum value in Eryou-6078. However, the capacity of potassium uptake (b value) by Kaiyou-5 ranked first and that by Shanyou-63 second. There was a significant linear relationship between nitrogen level and nitrogen uptake by rice, but a quadratic parabola relationship was found between nitrogen level and patassium uptake by rice. The application of nitrogen fertilizer decreased the ratios of potassium to nitrogen uptake by rice plant. The greatest reduction in the ratio was observed at high nitrogen level, and the least reduction was found in Kaiyou-5 and Shanyou-63 due to their greater ability to absorb potassium.     

可变电荷土壤和矿物Pb2+ and Cu2+吸附动力学: Ⅶ. Pb2+吸附后H+释放的动力学

The kinetics of H⁺ releasing after Pb²⁺ adsorption was studied by the potentiometric method combined with the acid-base titration curve technique. Results showed that pH of latosol, red soil and kaolinite suspensions dropped sharply, and then decreased gradually. Most of the H⁺ exchanged, more than 60%, was displaced at the first minute after Pb²⁺ was added into the suspension. More H⁺ was released at a higher concentration of Pb²⁺ added for a given sample and the amount of H⁺ released decreased in the order of red soil > latosol > kaolinite at a given concentration. The time-dependent data of H⁺ releasing of latosol, red soil and kaolinite at two concentrations could be fitted best with the second-order equation among the six equations, including second-order equation, two-constant equation, Elovich equation, parabolic diffusion equation, first-order equation and exponential equation. The H⁺/Pb²⁺ exchange stoichiometry increased with time, and a stable ratio of H⁺/Pb²⁺, being 1.73, 1.92 and 2.01 for kaolinite, latosol and red soil, respectively, was reached 25 minutes later.     

华中地区旱地氮素矿化的能力

Nitrogen mineralization potentials of 15 soil samples were studied by the methods of soil aerobic incubation, and the correlation between the potentials and the amounts of nitrogen taken up by rye grass (Lolium multiflorum Lam.) in pot culture was calculated. The soils were collected from Hubei Province in Central China. Soil nitrogen mineralization potentials (N_O) were calculated and optimized by a quick-BASIC program. N_O ranged from 60 mg kg^-1 to 340 mg kg^-1, which accounted for 9.1% to 34.6% of the total nitrogen content. Among the examined soils, yellow-brown soil collected from Wuhan had the largest N_O and brown-red soil from Xianning had the smallest one. The mineralization rate constants (k) ranged from 0.00556 d^-1 to 0.01280 d^-1, in average 0.00882 d^-1. Chao soil from Wuhan had the greatest k while yellow-cinnamon soil from Zhaoyang had the smallest one. There were apparent differences between mineralization parameters (N_O and k) optimized and non-optimized ones. Optimized N_O had a better correlation than non-optimized N_O with the amount of nitrogen accumulated in the aerial parts of rye grass. N_O, N_O × k and N_t(accumulated mineralized nitrogen within time t) could be used as indexes of soil nitrogen supply. Among them N_t was the best, which was significantly correlated with the amounts of nitrogen accumulated in the aerial parts of rye grass harvested at three different times.     

中国陕西省施有机肥黄土NH4+固定的热力学性质

Some thermodynamic properties of NH₄⁺ fixation by loess soil in plowing and clay layers are discussed. The results indicate that the four ion adsorption equations commonly used can describe the properties of NH₄⁺ fixation in these soils under constant temperature. Among the four adsorption equations, the single-surface Langmuir equation is the best. When the concentration of NH₄Cl solution is 10^-1 mol below, the Freundlich equation can be used. The changes of apparent standard free energy (ΔG°), enthalpy (ΔH°) and entropy (ΔS°) illustrate that NH₄⁺ fixation in soil is an endothermic adsorption and spontaneous reaction, and the process can be enhanced by a higher temperature and clay content in soil. The "proper value of NH₄⁺ fixation by soil (K₁ × q_m) increased with increasing clay content and temperature. The heat of NH₄⁺ fixation in soil (Q_m) confirms the conclusions made in this paper.     

接种食细菌线虫对小麦生长和N、P吸收的影响

A 40-day gnotobiotic microcosm experiment was carried out to quantify the effect of bacterial-feeding nematode on plant growth and nutrient absorption. The results showed that inoculation of bacterial-feeding nematode Protorhabditis sp. stimulated the growth of wheat (Triticum aestivum) and the uptake of N. By the end of the 40-day incubation wheat biomass and N uptake in the treatment with nematode and bacteria (Pseudomonas sp.) increased by 6.5% and 5.9%, respectively, compared with bacteria alone treatment. The presence of nematode mainly accelerated the growth of aboveground of wheat, while it slightly inhibited the root development. There was little difference in plant tissue N concentration between treatments. P concentration and uptake of wheat, however, were generally reduced by nematode. It appears that the enhancement of plant growth and nitrogen uptake is attributed to the enhancement of nitrogen mineralization induced by nematode feeding on bacteria, and the reduction of phosphorous uptake is the result of weak root status and competition by bacteria immobilization.     

玉米秸秆应用和腐化对钙质苏打土改良的影响: 实验室试验研究

A laboratory lysimeter experiment was conducted to investigate the effects of forage corn (Zea mays L.) stalk application on the CO₂ concentration in soil air and calcareous sodic soil reclamation. The experimental treatments tested were soil exchangeable sodium percentage (ESP) levels of 1, 11, and 19, added corn stalk contents of 0 to 36 g kg^-1, and incubation durations of 30 and 60 days. The experimental results indicated that corn stalk application and incubation significantly increased CO₂ partial pressure in soil profile and lowered pH value in soil solution, subsequently increased native CaCO₃ mineral dissolution and electrolyte concentration of soil solution, and finally significantly contributed to reduction on soil sodicity level. The reclamation effciency of calcareous sodic soils increased with the added corn stalk. When corn stalks were added at the rates of 22 and 34 g kg^-1 into the soil with initial ESP of 19, its ESP value was decreased by 56% and 78%, respectively, after incubation of 60 days and the leaching of 6.5 pore volumes (about 48 L of percolation water) with distilled water. Therefore, crop stalk application and incubation could be used as a choice to reclaim moderate calcareous sodic soils or as a supplement of phytoremediation to improve reclamation effciency.     

不同液土比和温度条件下塿土铵离子吸附

Effects of NH₄⁺ concentration, solution/soil ratio and temperature on NH₄⁺ adsorption were studied in a Eum-Orthic Anthrosol. The slopes of the soil NH₄⁺ adsorption isotherms and the fitted n, the coefficient for the adsorption intensity, and k, the coefficient related to adsorption capacity, of the Freundlich equation increased with increasing solution/soil ratio (SSR) and with decreasing temperature (T). For the range of experimental conditions, the value of ∂q/∂c, the rate of change of the amount of NH₄⁺ adsorbed in the soil solid phase (q) with respect to the equilibrium concentration of NH₄⁺ in soil solution (c), was 0.840, indicating that q increased with increasing c. From 2 to 45 ℃, ∂q/∂SSR, the rate of change of q with respect to SSR, decreased from 2.598 to 1.996, showing that q increased with increasing SSR, while its increasing rate decreased with temperature. From SSR 1:1 to 20:1, ∂q/∂T, the rate of change of q with respect to T, decreased from -0.095 to -0.361, indicating that q decreased with increasing temperature, and at the same time the negative effect of temperature became larger as SSR increased. Thus under the experimental conditions the order of importance in determining the amount of NH₄⁺ adsorbed in the soil solid phase was ∂q/∂SSR > ∂q/∂c > |∂q/∂T|, indicating that the greatest effect on the amount of NH₄⁺ adsorbed was with the solution/soil ratio; the equilibrium concentration of NH₄⁺ had a lesser effect; and temperature had the least effect.     

Soil Erosion as A ffected by Polyacrylamide Application Under Simulated Furrow Irrigation with Saline Water

The reduction of soil and water losses under furrow irrigation with saline water is important to environmental protection and agricultural production.The objective of this study was to determine the effect of polyacrylamide(PAM)application on soil infiltration and erosion under simulated furrow irrigation with saline water.Polyacrylamide was applied by dissolving it in irrigation water at the rates of 1.5,7.5,and 15.0 mg L^-1 or spreading it as a powder on soil surface at the rates of 0.3,1.5,3.0,and 6.0 g m^-2,respectively.The effectrolyte concentration of tested irrigation water was 10 and 30 mmolc L^-1 and its sodium adsorption ratio(SAR)was 0.5,10.0,and 20.0(mmol_c L^-1)_0.5.Distilled water was used as a control for irrigation water quality.Results indicated that the effectrolyte concentration and SAR generally did not significantly affect soil and water losses after PAM application.Infiltration rate and total infiltration volume decreased with the increase of PAM application rate.Polyacrylamide application in both methods significantly reduced soil erosion,but PAM application rate did not significantly affect it.The solution PAM application was more effective in controlling soil erosion than the powdered PAM application,but the former exerted a greater adverse influence on soil infiltration than the latter.Under the same total amounts,the powdered PAM application resulted in a 38.2%-139.6% greater infiltration volume but a soil mass loss of 1.3-3.4 times greater than the solution PAM application.     

直链烷基苯磺酸盐(LAS)在土壤-植物系统中的生态学行为研究

More and more linear alkylbenzene sulfonate (LAS) has contaminated the water and soil via pollution discharge, making it important to identify the ecological behavior and toxicity of LAS so as to carry out measures that will reduce its negative effects on the ecosystem. The ecological behavior of LAS, including degradation, migration, and plant uptake, in both soil-paddy rice and soil-soybean systems was studied. Reduction of LAS in pot and field plots followed the first order reaction kinetics with degradation half-lives of 35-50 days with LAS decreasing to very low concentrations after a season of crop growth. Strong migration ability for LAS was found and the breakthrough time in a 1.5 m soil monolith was significantly shortened to 23 days by preferential flow. Leachate volumes of soil-paddy and soil-soybean systems at preferential breakthrough were much different, while the leachate volumes at equilibrium governed by soil adsorption/desorption processes were very similar. Significant uptake of LAS in both paddy rice and soybeans was observed in pot and field experiments (P < 0.05). In aquatic culture, 20 ug mL^-1 and above of LAS significantly inhibited the growth of paddy seedlings (P < 0.05). The critical concentration for LAS in soil inhibiting the growth and yield of paddy was 160 ug g^-1; when higher, there was a strong negative influence, with decreases in height, spike length, and production; when lower than 80 ug g^-1, paddy growth was stimulated. There was little effect of LAS on soybeans.     

Arbuscular mycorrhizal fungi and endophytic fungi differentially modulate polyamines or proline of peach in response to soil flooding

Symbiotic fungi are involved in plant flooding tolerance, while the underlying mechanism is not yet known. Since polyamines (PAs) and proline are also associated with stress tolerance, it is hypothesized that the enhancement of stress resistance by symbiotic fungi is associated with changes in PAs and/or proline. The aim of this study was to analyze the effect of inoculation with Funneliformis mosseae and Serendipita indica on plant growth, PAs, and proline and the metabolisms in peach (Prunus persica) under flooding. Two-week flooding did not affect root colonization frequence of F. mosseae, while it promoted root colonization frequence of S. indica. Under flooding, plants inoculated with F. mosseae and S. indica maintained relatively higher growth rates than uninoculated plants. Funneliformis mosseae promoted root ornithine (Orn) contentration and arginine (Arg) and Orn decarboxylase activities under flooding, which promoted putrescine (Put), cadaverine (Cad), and spermidine (Spd) contentrations. Conversely, S. indica decreased contentrations of Arg, Orn, and agmatine and Arg decarboxylase activities, thus decreasing PA contentrations under flooding. Polyamines were negatively correlated with the expression of PA uptake transporter genes, PpPUT1 and PpPUT2, in peach. Polyamine transporter genes of F. mosseae (FmTPO) and S. indica (SiTPO) were regulated by flooding, of which FmTPO1 was positively correlated with Put, Cad, and Spd, along with positive correlations of Spd with SiTPO1, SiTPO2, and SiTPO4. Under flooding, F. mosseae decreased proline concentration, while S. indica increased proline concentration and correlated with expression of a △¹-pyrroline-5-carboxylate synthetase gene, PpP5CS2. It was thus concluded that F. mosseae modulated polyamine accumulation, while S. indica induced proline accumulation to tolerate flooding.     

大气CO2浓度升高对黄花蒿生长和氮磷钾养分吸收的影响

Annual wormwood (Artemisia annua L.) is the only viable source of artemisinin, an antimalarial drug. There is a pressing need to optimize production per cultivated area of this important medicinal plant; however, the effect of increasing atmospheric carbon dioxide (CO₂) concentration on its growth is still unclear. Therefore, a pot experiment was conducted in a free-air CO₂ enrichment (FACE) facility in Yangzhou City, China. Two A. annua varieties, one wild and one cultivated, were grown under ambient (374 μmol mol^-1) and elevated (577 μmol mol^-1) CO₂ levels to determine the dry matter accumulation and macronutrient uptake of aerial parts. The results showed that stem and leaf yields of both A. annua varieties increased significantly under elevated CO₂ due to the enhanced photosynthesis rate. Although nitrogen (N), phosphorus (P), and potassium (K) concentrations in leaves and stems of both varieties decreased under elevated CO₂, total shoot N, P, and K uptake of the two varieties were enhanced and the ratios among the concentrations of these nutrients (N:P, N:K, and P:K) were not affected by elevated CO₂. Overall, our results provided the evidence that elevated CO₂ increased biomass and shoot macronutrient uptake of two A. annua varieties.     

紫色土施用磷酸盐肥料对玉米铜毒害的缓解

A pot experiment was conducted to evaluate the effects of phosphorus fertilizer in inhibiting the copper toxicity to maize(Zea mays L.) in neutral purple soil. Results indicated that the growth of the shoot and roots of maize plant was obviously reduced by copper and the height and biomass were significantly negatively correlated to the application levels of copper (r = -0.899^**~-0.994^**) at no P and low P (100 mg kg^-1). However, the maize biomass was relatively increased and the high Cu (100 and 200 mg kg^-1) induced toxicity of maize was greatly alleviated in all treatments with medium P (300 mg kg^-1 ) and high P (500 mg kg^-1). To maintain the normal growth of maize plant (≥3.68 g pot^-1), the critical application rates of phosphorus fertilizer should be 160, 210, 300 and 500 mg P kg^-1 at 10, 50, 100 and 200 mg Cu kg^-1 levels of the soil, respectively. The increases in polyphenol oxidase and catalase activities in maize leaf and dehydrogenase activity in roots by phosphorus fertilizer were in the order of medium P>high P>low P>no P. Activities of polyphenol oxidase and catalase were significantly positively correlated to the application levels of copper (r =0.892^**~0.924^**), whereas that of dehydrogenase was just reverse (r = -0.966^**) at no P. Medium and high P repressed the influence of copper on activities of three enzymes. Phosphorus fertilizer reduced the copper concentrations of maize roots and leaf and the change ranges of the P/Cu ratio of maize roots and the P/Cu, N/Cu and K/Cu ratios of maize leaf. The three ratios of maize leaf were 256^**71.5, 2643^**839 and 1133^**440 at normal growth of maize plant, respectively. Soil available Cu could be markedly cut down by application of phosphorus fertilizer, especiallly at high phosphorus level.     

土壤结构组成对K+运移障碍因子的定量研究

With six packed columns composed of <1 μm and 5 μm~0.25 mm fractions from an Eum-Orthic Anthrosol (Columns 1~6) and one column of the Eum-Orthic Anthrosol (Column 7), K⁺ transport experiments under the condition of saturated steady water flow were conducted to qualify the effects of soil texture composition on the retardation factor (R) of K⁺ transport. The results showed that the retardation factor of K⁺ transport in the tested soil columns greatly increased with increasing clay contents. In an attempt to use pedo-transfer function (PTF) approach in the solute transport study, a preliminary PTF was established through the six packed columns (Columns 1~6) with soil basic data including soil bulk density, volumetric water content and clay content to predict the retardation factor, and proved valid by the satisfactory prediction of R in Column 7.