Alley cropping rice (Oryza sativa var.Barafita) with cassia (Cassia siamea): soil fertility and crop production

Rice grain weight and quality (weight of larger size fractions), soil pH, and N, P, K and organic matter content were studied where rice was alley cropped with cassia on a semiarid site in The Gambia. The four treatments, control (no cassia prunings or inorganic fertilizer added), only prunings added, prunings plus half the recommended fertilizer rate and prunings plus full recommended rate of fertilizer, were applied in a Latin square design with 10×8 m plots, each sub-divided into two 4-m wide alleys. Fertilizer was applied twice; full rates were 93.7 kg/ha NPK (8:24:24) plus 32.4 kg/ha urea followed two weeks later by 100 kg/ha urea applied as side dressing. Soil samples collected before and after cropping at 0–10 cm and 10–15 cm depths and cassia pruning samples were analyzed for pH, N, P, K and organic matter content.Our results do not show significant benefit of cassia prunings applied as mulch to grain weight or quality in alley cropping rice with cassia. The addition of inorganic fertilizer plus cassia prunings did not increase rice grain and straw weights (p=0.3447 and p=0.0691, respectively) compared to the control and prunings only treatments. In all treatments, the outer rows, those within 80 cm of hedgerows, produced significantly less grain (p=0.0002) and straw than inner rows. Neither the larger grade A nor the smaller grade B grain weights were significantly different (p=0.6017 and p=0.0629, respectively) between treatments. Weight of grain, straw, and larger grade A and smaller grade B quality grain did not differ significantly for inner and outer rows (p=0.6329, p=0.7148, p=0.7171 and p=1.000, respectively).     

Potentials of Millettia thonningii and Pterocarpus santalinoides for alley cropping in humid lowlands of West Africa

This study was conducted to assess the suitability of two fallow species that are indigenous to West Africa, M. thonningii (Schum and Thonn) and P. santalinoides (L'Her), for alley cropping with maize and their effect on soil chemical properties. It was carried out during the rain-fed cropping season at Ibadan, Nigeria and Mbalmayo, Cameroon in 1993 and 1994. Total dry matter of P. santalinoides prunings was higher at the two sites than that of M. thonningii by about 35% to 37%. Maize grain yield in plots supplied with prunings was significantly higher (P > 0.05) than in control (no prunings or fertilizer application) at Ibadan. Grain yield in plots supplied with prunings plus 40 kg ha⁻¹ urea fertilizer gave significantly higher yields than plots supplied with 80 kg N ha⁻¹ urea fertilizer only. At Mbalmayo, there was no significant difference between grain yield in plots supplied with 80 kg N ha⁻¹ and plots supplied with prunings plus 40 kg N ha^-1 urea fertilizer though the latter had higher yields. Grain yield was also higher in the middle rows than in rows adjacent to the hedgerows and these were not significantly different. Weed dry matter was reduced by 27% to 43% when Pterocarpus prunings were applied and 13% to 31% with application of Millettia prunings. Weed flora in both locations changed from grasses to broad leaved. Soil chemical changes at soil depth 0 to 10 cm showed significant increases (pH, C, N, P and Ca) after two cropping seasons in plots supplied with prunings or prunings plus fertilizer than the initial values. At Mbalmayo, K was lower after cropping in treatments than the initial values while at Ibadan, K and Mg were lower except in plots supplied with Pterocarpus prunings only. P. santalinoides and M. thonningii have significant potential for agroforestry in this sub-region. This revised version was published online in June 2006 with corrections to the Cover Date.     

Growth and yield responses of cotton (<Emphasis Type="Italic">Gossypium hirsutum</Emphasis>) to inorganic and organic fertilizers in southern Malawi

Fertilizer trees, the nitrogen-fixing legumes, such as gliricidia (Gliricidia sepium) and tephrosia (Tephrosia spp.) have been used to improve soil fertility for higher crop yields in nitrogen deficient soils. Many studies have focused on how these fertilizer trees improve maize yield, but there has been a dearth of information on the effect of fertilizer tree species on cotton growth and yield. A study was undertaken for two cropping seasons (2012/13 and 2013/14) with the objective of assessing IRM 81 cotton growth and yield responses to tephrosia and/or gliricidia biomass with or without inorganic fertilizer application. Boll opening significantly varied (P < 0.0001) with treatments and early boll opening was observed in plots where only inorganic fertilizer was applied. Higher lint yield (mean of 1397 kg/ha) was obtained in the second cropping season than in the first cropping season (480 kg/ha) and the application of gliricidia biomass with fertilizer gave the highest lint yield (2121 kg/ha). The lowest lint and seed yields were obtained from plots where tephrosia biomass only was applied. It is concluded that the use of gliricidia biomass with inorganic fertilizer improved cotton yields. The high amount of gliricidia biomass (due to prolific coppicing) applied contributed to higher cotton lint yields with reduced rates of inorganic fertilizer application, making gliricidia-cotton intercropping a cost-effective option to smallholder farmers.     

Calliandra calothyrsus (Meissn.) in an alley cropping system with sequentially cropped maize and cowpea in southwestern Nigeria

Alley cropping is increasingly becoming accepted as an appropriate technology with the potential to provide stable and sustainable food production in the tropics. However, only a few of the potential trees/shrubs have been tested. The performance of Calliandra calothyrsus (Meissn) as an alley cropping species was evaluated on an Oxic Paleustalf. The treatments were: prunings removal, prunings application; and three N levels, 0,45, and 90 kg N ha^–1, in a factorial arrangement. The cropping sequence was maize (main season) follwed by cowpea (minor season). Nitrogen fertilizer treatments were applied to the maize crop only. Four annual prunings of Calliandra hedgerows produced a total of 6 t ha^–1 of dry matter prunings containing about 200 kg ha^–1 of N. Maize yields were increased by the application of prunings but no benefits were obtained by supplementing the prunings with inorganic N. An average maize grain yield of 3.1 t ha^–1 per year was maintained without any chemical fertilizer input. However, without any prunings, maize yields were substantially increased by the application of inorganic N. Cowpea yield did not respond to application of prunings but plants grown adjacent to the hedgerows had reduced yield probably due to shading. Our results suggest that six rows (0.57 m inter-row spacing) between Calliandra hedgerows spaced at 4 m are optimum for this cowpea variety. The performance of Calliandra was comparable to that of Leucaena which has been widely shown to be effective in alley cropping systems of the region.     

Growth and water relations of Eucalyptus marginata (jarrah) stands in response to thinning and fertilization

We studied the effects of five thinning treatments (T1 = 5.5, T2 = 11, T3 = 16.5, T4 = 22.5 and T5 = 28.5 m(2) ha(-1) basal area under bark) x two fertilizer treatments (F0 = unfertilized and F1 = fertilized with 400 kg ha(-1) N plus 229 kg ha(-1) P) on growth and water relations of pole-sized Eucalyptus marginata J. Donn ex Sm. trees growing in southwestern Australia. Thinning reduced leaf area index (LAI) from 2.1 in the T4 and T5 treatments to 0.8 in the T1F0 treatment. Fertilizer had no effect on LAI in the T2, T4 or T5 treatments, but increased LAI by 45 and 20% in the T1 and T3 treatments, respectively. Thinning plus fertilizing increased diameter growth most in the fastest growing trees, from 0.4 cm year(-1) for trees in the T5F0 and T5F1 treatments to 0.7 and 1.2 cm year(-1) for trees in the T1F0 and T1F1 treatments, respectively. In both fertilizer treatments, stand basal area and volume growth increased with increasing stand density up to 15 m(2) ha(-1), and thereafter declined with increasing stand density, such that the growth rate of trees in the T5 treatment was only half of that at a stand density of 15 m(2) ha(-1). In response to fertilizer, growth rates of the slowest and fastest-growing trees increased from 0.35 and 3.5 m(2) ha(-1) year(-1) (F0) to 0.56 and 5.4 m(3) ha(-1) year(-1) (F1), respectively. Stand growth efficiency (growth per unit LAI) increased in response to thinning, and fertilizer increased stand growth efficiency at all stand densities. Throughout the dry season, T5 trees had lower predawn shoot water potentials (Psi(pd)) (minimum of -1.5 MPa) than T1 or T2 trees (minimum of -0.7 MPa). Fertilizer decreased Psi(pd) in T5 trees (by -0.9 and -1.5 MPa, respectively, in F0 and F1), but not in T1 or T2 trees. Stand growth rate was closely related to cumulative midday water stress (CMWS) over the dry season, and volume growth rate declined sharply from 6 m(3) ha(-1) year(-1) at a CMWS of 130 MPa days, to zero at a CMWS of 220 MPa days. Application of fertilizer to thinned stands increased LAI, stand growth efficiency and stand growth. In unthinned stands, fertilizer increased stand growth efficiency and stand growth; however, it also increased tree water stress, which limited the fertilizer-induced increases in LAI and growth. We attribute the increase in tree and stand growth in response to application of fertilizer to increased photosynthetic rates, increased allocation to stem wood, and in thinned stands also to higher LAIs.     

Defining competition vectors in a temperate alley cropping system in the midwestern USA: 3. Competition for nitrogen and litter decomposition dynamics

An experiment was conducted in an 11-year-old black walnut (Juglans nigra L.), red oak (Quercus rubra L.), maize (Zea mays L.) alley cropping system in the midwestern USA to examine the extent of tree-crop competition for nitrogen and decomposition dynamics of tree leaves and fine roots. A below-ground polyethylene root barrier (1.2 m deep) isolated black walnut roots from maize alleys in half the number of plots providing two treatments viz. barrier and no barrier. The percentage of N derived from fertilizer (%NDF) and fertilizer use efficiency (%UFN) were determined using ¹⁵N enriched fertilizer. Further, maize grain and stover biomass, tree leaf biomass, tissue N concentration, and N content were quantified in both treatments. The barrier treatment resulted in a significantly greater grain (67.3% more) and stover (37.2% more) biomass than the no barrier treatment. The %NDF in both grain and stover was higher in the no barrier treatment as a result of competition from tree roots for water and mineralized N in soil. Maize plants growing in the no barrier treatment had a lower %UFN than those in the barrier treatment due to their smaller size and inability to take up fertilizer. Analysis of tree leaf and fine root decomposition patterns revealed faster release of N (39% over 15 days for black walnut and 17.7% for red oak) and P (30% over 15 days for both species) from roots compared to the leaves of both species. Following an early release of P (11.3% over 45 days), red oak leaves exhibited significant immobilization for the rest of the incubation period. The data indicate that competition for N from fertilizer is minimal since nutrient acquisition is not simultaneous among black walnut and maize. However, competition for mineralized N in soil can exist between black walnut and maize depending on water availability and competition. Tree leaves and fine roots can enhance soil nutrient pools through the addition of soil carbon and nutrients. Tree fine roots seem to play a more significant role in nutrient cycling within the alley cropping system because of their faster release of both N and P as compared to leaves. Selection of tree species and their phenology will impact the magnitude and rate of nutrient cycling.This revised version was published online in November 2005 with corrections to the Cover Date.     

Multipurposes tree prunings as a source of nitrogen to maize under semiarid conditions in Zimbabwe

Time of pruning application and mixing prunings of varying qualities could be important management options to increase the rate of nitrogen recovery from multipurpose-tree prunings that are used as a source of nitrogen to crops. A field experiment was conducted in the semiarid zone of Zimbabwe to test this hypothesis, using prunings of calliandra (Calliandra calothyrsus) and leucaena (Leucaena leucocephala), alone and in mixtures, and maize (Zea mays) as the test crop. Time of pruning application significantly improved N uptake, N recovery, and grain yield of maize. Applying prunings of calliandra at maize planting was significantly better in terms of N uptake, N recovery, and grain yield than applying them four weeks after planting. However, with leucaena, time of prunings application had no significant effects on N recovery. Mixing prunings of leucaena and calliandra had no effect on maize N uptake, N recovery, and grain yield. Split application of available prunings during the crop growth cycle had no effect on N recovery compared to one-time application of entire amounts of prunings at planting.     

Vibrational property changes of spruce wood by impregnation with water-soluble extractives of pernambuco (Guilandina echinata Spreng.)

Sitka spruce (Picea sitchensis Carr.) was treated with water-soluble extractive components of pernambuco (Guilandina echinata Spreng. syn Caesalpinia echinata Lam.) by two methods: impregnation under evacuation using an aspirator and repetitive surface application using a brush. The influence of these treatments on the vibrational properties were examined. The loss tangent (tan ) of the impregnated specimen decreased, up to nearly a half of its original value, with increasing weight gain. It is suggested that the decrease in tan results from impregnation of the extractive components into the amorphous region of cell walls, forming secondary bonds between matrix substances. The surface application of the extractive components, on the other hand, hardly brought about the desirable change in vibrational properties.Part of this work was presented at the 47th annual meeting of the Japan Wood Research Society, Kochi, April 1997, and the 48th annual meeting of the Japan Wood Research Society, Shizuoka, April 1998     

Nitrogen contribution of five leguminous trees and shrubs to alley cropped maize in Ibadan, Nigeria

There are abundant local legume trees and shrubs potentially suitable for alley cropping systems in the sub-Saharan Africa, which are yet to be studied. The nitrogen contribution of two years old Albizia lebbeck and S. corymbosato yield of maize grown in alley cropping was compared to that of Senna siamea, Gliricidia sepium and Leucaena leucocephala in four seasons at Ibadan. Maize shoot biomass and maize grain yield in A. lebbeck alley compared favourably with that in G. sepium and L. leucocephala. Maize biomass and grain yield in S. corymbosa alleys were the lowest. Within A. lebbeck, L. leucocpehala, and G. sepium alleys there were no significant differences in the maize yield in the alleys that received 0, 40 or 80 kg N/ha. Application of more than 40 kg N/ha in S. corymbosa alleys was not necessary as there was no significant increase in maize yield at the higher level of nitrogen. Maize yield and N uptake in A. lebbeck alleys were not significantly different from yield and N uptake in G. sepium, and L. leucocephala at the same fertilizer level. There was a significant correlation between hedgerow tree biomass and maize grain yield. At the end of twelve weeks after pruning application, the organic residues of the pruning applied in the alleys ranged from 5% in G. sepium and 44% in A. lebbeck in the first year compared with the original pruning applied which showed that the slow rate of A. lebbeck decomposition could have a beneficial effect on the soil. The maize N recovery from applied N fertilizer was low (10–22%). Percentage N recovery from the prunings was low in the non-N fixing trees (12–22%), while the recovery was high (49–59%) in A. lebbeck as well as in the other nitrogen fixing tree prunings. Thus A. lebbeck, apart from enhancing maize growth and grain yield like in L. leucocephala and G. sepium, had an added advantage because it remained longer as mulching material on the soil because of its slow rate of decomposition. It was able to survive pruning frequencies with no die-back. This indicates that A. lebbeck is a good potential candidate for alley cropping system in West Africa. S. corymbosa performed poorly compared with the other legume trees. Though it responded to N fertilizer showing a positive interaction between the hedgerow and fertilizer application, it had a high die back rate following pruning periods and termite attack.This revised version was published online in November 2005 with corrections to the Cover Date.     

Improving maize production through nitrogen supply from ten rarely-used organic resources in Ghana

Where there is limited availability of conventional fertilizers, the use of organic materials is considered a viable alternative to increase the productive capacity of soils. Many potential plant residues remain underutilized due to limited research on their use as a nutrient source. In this study, the nitrogen supplying capabilities of ten rarely-used leaf biomass sources (Acacia auriculiformis, Baphia nitida, Albizia zygia, Azadirachta indica, Senna siamea, Senna spectabilis, Tithonia diversifolia, Gliricidia sepium, Leucaena leucocephala and Zea mays) were tested based on their nutrient content, N mineralization patterns and effect on maize yield (in comparison with inorganic fertilizer). N mineralization was studied in the laboratory using an incubation experiment. Field trials were also established using a randomized complete block design. Plant residues were applied at 5 t dry matter ha^?1 a week before planting maize while fertilizer was split-applied at 90 kg N ha^?1 on designated plots. From the results on plant residue chemistry, most of the plant residues recorded relatively high N concentration (≥24.9 g kg^?1) and low C/N ratio (≤20.1) although neither N content nor C/N ratio significantly (p > 0.05) affected their N mineralization patterns. Leaf biomass application of B. nitida, A. auriculiformis, A. zygia and maize stover resulted in an initial net N immobilization that lasted for 14 days. Application of all plant materials significantly increased the biological yield and N uptake of maize with G. sepium and T. diversifolia producing the greatest impact especially in the major rainy season. Relative to the control, total grain yield after four cropping seasons was comparable between inorganic fertilizer (9.2 t ha^?1), G. sepium (8.8 t ha^?1) and T. diversifolia (9.4 t ha^?1) treatments. The results on maize biological yield were significantly correlated with the effects of the treatments on N uptake. The findings suggest that in locations where inorganic fertilizers are limited, leaf biomass from G. sepium and T. diversifolia could offer the most suitable option in comparison with the other species used in this study.     

Cross sectional dimensions of wood pulp fibers from softwood fiber counts

Summary There are, on the average, about 300 fibers or tracheids per centimeter in both the tangential and radial directions in cross sections of softwoods when the counts are made over complete annual rings with the extremes varying from about 200 fibers per centimeter for redwood to about 400 fibers per centimeter for Alaska yellow cedar. Fiber widths, including half of surrounding middle lamella, range from about 50 m for redwood to 25m for Alaska yellow cedar, averaging about 33 m. Average lumen widths vary but slightly with changes in the specific gravity of the wood whereas the double cell wall thickness varies directly with the specific gravity. Effects of pulping to different pulp yields on the fiber dimensions are calculated with the use of two newly developed equations. The theoretical thickness of completely collapsed pulp fibers is equal to their double cell wall thickness. Complete collapse rarely if ever occurs as it requires double fractures of the cell wall. In practice, collapse of fibers is incomplete so their thickness is usually two or more times the theoretical minimum.Emeritus Robertson Professor of Wood and Paper Science, North Carolina State University, Raleigh, N.C., U.S.A.     

Pattern of soil moisture depletion in alley cropping under semiarid conditions in Zambia

The pattern of soil moisture changes was studied during a cropping season in an alley cropping experiment of maize withLeucaena leucocephala andFlemingia macrophylla at the SADC/ICRAF Agroforestry Research Station in a semiarid region near Lusaka, Zambia (28°2956 East and 15°2132 South). Soil moisture potential was monitored at regular intervals using tensiometers installed at 15, 30 and 45 cm depths in fertilized and unfertilized alleys within the double hedgerow, and the first, second and third rows of maize in the alleys.Soil moisture moved mostly towards the top horizon during very dry conditions. Alleys that had received a combination of fertilizer and hedgerow prunings depleted more moisture than those that had only hedgerow prunings. There were no differences in moisture utilization pattern between leucaena and flemingia hedgerows. The hedgerows depleted the same amount of moisture as the maize plants. However, during dry conditions, there was a higher soil moisture content under the hedgerows than in maize rows, indicating that there was no apparent competition for moisture between the hedgerows and the maize plants.     

Alley cropping of maize with calliandra and leucaena in the subhumid highlands of Kenya: Part 2. Soil-fertility changes and maize yield

Although N-rich leaf biomass of multipurpose trees is known to be a good source of N to crops, integrating such trees into crop production systems is a major challenge in the development of viable agroforestry systems. An approach to integrating calliandra (Calliandra calothyrsus Meissner) and leucaena (Leucaena leucocephala (Lam.) de Wit), two promising agroforestry tree species, into maize (Zea mays L.) production system was investigated in the subhumid highlands of central Kenya during four maize-growing seasons from 1994 to 1996. The experiment consisted of maize plots to which tree prunings obtained from hedgerows grown either in situ (alley cropping) or ex situ (biomass transfer from outside) were applied. When alley-cropped with leucaena, maize produced significantly higher yields compared to maize monoculture (both non-fertilized and fertilized) treatments, but when alley-cropped with calliandra, the yield of maize was less than that of the monocropped unfertilized control. Application of ex situ grown calliandra and leucaena prunings with or without fertilizer resulted in higher maize grain yield than in the nonfertilized and fertilized treatments. Yields of calliandra alley- cropped maize were 11% to 51% lower than those of nonalley-cropped treatments receiving calliandra prunings from ex situ grown trees; the decrease was 2% to 17% with leucaena, indicating that calliandra hedges were more competitive than leucaena hedges. The alley-cropped prunings-removed treatments produced the lowest maize yields. The study showed that, in the subhumid tropical highlands of Kenya, inclusion of calliandra hedges on cropland adversely affected maize yields. On the other hand, alley cropping with leucaena was advantageous. This revised version was published online in June 2006 with corrections to the Cover Date.     

Nitrogen mineralization and maize yields following application of tree prunings to a sandy soil in Zimbabwe

Despite the promotion of prunings as sources of nitrogen for crops, lack of synchronization between N mineralization from prunings and plant uptake remains a major limitation to the impact of prunings on crop yields. A laboratory and a field experiment were therefore carried out to determine the mineralization patterns of selected prunings and assess the potential that exists to improve synchrony by mixing prunings of different quality. The laboratory incubation experiment was conducted for 84 days to determine the C and N release patterns of prunings of different quality and the manipulation of the C and N mineralization trends by mixing prunings of different quality. High quality prunings were considered to be those with high CO₂ evolution rates and mineralize N rapidly. The % C and % N released in 84 days were highest for the high quality prunings of Tithonia diversifolia (70% and 30% respectively) and least for the low quality prunings of Flemingia macrophylla (25% and –5% respectively). The medium quality prunings of Acacia angustissima and Calliandra calothyrsus had similar proportions of released C and N (about 40% C and 10% N). Different mixtures of T. diversifolia with other species showed contrasting influence on C release and N mineralization. Most mixtures released less C than that predicted, but in contrast most mixtures released N at a rate either matching or above the predicted. The % N released was strongly correlated with the polyphenol protein binding capacity (r² = 0.53) and also with the % C released (r² = 0.62). The field experiment was done for two cropping seasons to determine the effects of the prunings of these species and their mixtures on maize grain yield. The prunings were added at 5 t ha^–1 and incorporated into the top 15 cm by hand hoeing in the first season and their residual effects were monitored in the second season. Medium and low quality prunings produced significantly (p < 0.05) higher maize grain yields in the first season ranging between 2.4 t ha^–1 and 3.4 t ha^–1 compared with T. diversifolia which produced 1.7 t ha^–1. This suggested better synchrony in N release and uptake by maize with medium and low quality prunings compared with high quality prunings. The only mixture that indicated improved synchrony was the mixture of T. diversifolia and C. calothyrsus. This study showed that mixing prunings of different quality produce different patterns of N mineralization, some of which were unexpected and had a potential for improving N synchrony.This revised version was published online in November 2005 with corrections to the Cover Date.     

Control of the American dog tick,Dermacentor variabilis Say (Acarina: Ixodidae)

In a 4 ha area, of which 1/3 was meadow vole habitat, the old vole runways were spot treated in early spring with a 1% spray of flowable Sevin^® 4F (42% AI) on ca 4 m centers. New runways, as noted, were treated in June. The application averaged 60 ml of Sevin^® 4F/ha. The spring adult cohort ofDermacentor variabilis was <10% of that usually expected. These adults had been exposed to this treatment the previous year as spring larvae and summer nymphs. The reduction of the adult population occurred in a season of heavy infestation. This treatment is not labor intensive and requires only a minimum of pesticide because it is directed to its target, the attached immatures, by the behavior of the field mice. It should be noted that all the small rodent hosts of the immatures use the vole runways.     

Alley cropping of maize with nine leguminous trees

A maize-leguminous tree alley cropping system was studied on N-deficient soils in Hawaii to determine mulch effects on maize yields. Calliandra calothyrsus, Cajanus cajan, Cassia siamea, Gliciridia sepium, KX1 — Leucaena hybrid (L. pallida X L. diversifolia), L. leucocephala, L. pallida, L. salvadorensis, and Sesbania sesban were evaluated for green manure and yield of intercropped maize. S. sesban, G. sepium, L. pallida, and KX1 produced between 5 and 12 dry t/ha/yr green manure with nitrogen yields between 140 and 275 kg N/ha in 4 prunings. Maize yields responded linearly to nitrogen applied as green manure. Maize yield increased 12 kg for each kg of nitrogen applied. Additions of prunings from hedge rows were able to support maize grain yields at about 1800 kg/ha for two consecutive cropping seasons, while control plot yields averaged less than 600 kg/ha. Maize yields reflected the amount of nitrogen applied as green manure, regardless of tree species from which the nitrogen was derived. In March, maize yields decreased 34% in the row spaced 40 cm from the hedge, relative to the one spaced 110 cm away. In July, increasing the distance away from the hedge to 60 cm and coppicing the hedge earlier in maize growth, significantly improved grain yield. Grain yields decreased only 10% in the row closest to the trees.     

Adsorption capacities and related characteristics of wood charcoals carbonized using a one-step or two-step process

Sugi (Cryptomeria japonlca D. Don) wood powder was carbonized at varying temperatures by a onestep process up to 1000C and a two-step process using wood charcoal as the raw material up to 1600C. This study was conducted to evaluate the adsorptive properties of wood charcoal and discuss the mechanism of its adsorptive function in relation to the physical and anatomical characteristics of wood after carbonization. Anatomical characteristics of carbonized wood materials were directly observed under heating using an environmental scanning electron microscope (ESEM); the cell wall structures were analyzed by high-resolution transmission electron microscope (HRTEM). The largest weight losses were observed at the highest temperatures, in both the one-step and twostep processes but leveled off above 800C. Shrinkages in the tangential, radial, and longitudinal directions increased with carbonization temperature, peaking at 1000C. Direct observations by ESEM showed distinct shrinkage at around 400C. The first trial observations by HRTEM on the changes in the ultrastructure of cell walls of wood charcoals were done, and it was assumed to affect the formation of micropores. Adsorption was found to follow the Langmuir isotherm model. With the one-step carbonization process, the iodine adsorption capacities of the carbonized wood powders increased with increasing carbonization temperature, peaking at 800C, but decreased at higher temperatures. The wood powder carbonized at 1000C with the two-step process showed the highest capacity, but further heating up to 1400C drastically decreased the adsorption. The shrinkage of cells was related to the increases and decreases in its specific surface area. Specific surface area and total pore volume were evidently related to the adsorptive properties.Part of this paper was presented at the Second International Wood Science Seminar, Indonesia, November 6–7, 1998     

Screening of heterozygous DNA markers in shiitake (Lentinula edodes) using de-dikaryotization via preparation of protoplasts and isolation of four meiotic monokaryons from one basidium

A suitable screening method for heterozygous DNA markers in shiitake,Lentinula edodes (Berk.) Pegler, is reported. Monokaryons were derived from a dikaryon by de-dikaryotization via protoplast formation. Compatibility of the monokaryons was determined by pairwise culture on agar plates. We selected the primers to amplify polymorphic fragments among the original strain (Hokken600H600) and two monokaryons (H600PP-39 and H600PP-67) showing compatibility. A total of 135 fragments were selected as specific random amplified polymorphic DNAs (RAPDs) resulting from 56 primers of the 147 primers tested. Furthermore, we tested whether the polymorphic fragments segregated into 22 among four strains isolated from a basidium. Most of the polymorphic fragments (about 97.8%) showed 22 segregation among the four strains. We concluded that the polymorphic fragments were heterozygous if they were detected in either of the monokaryons (H600PP-39 and H600PP-67) and segregated to 22 among four meiotic strains (H600B-1,-2, -3, and -4). A total of 132 heterozygous DNA markers were therefore selected from a dikaryon of shiitake (Hokken600H600).Part of this report was presented at the 8th Annual Meeting of Mushroom Science and Biotechnology and the 47th Annual Meeting of the Japan Wood Research Society, Kochi, 1997     

Multipurpose tree prunings as a source of nitrogen to maize under semiarid conditions in Zimbabwe

When multipurpose-tree (MPT) prunings are used as a source of nitrogen to annual corps in agroforestry systems, it is important that high levels of N recovery are attained. In order to test the effect of pruning quality and method of pruning application on N-recovery rates, a field experiment was conducted using prunings of five MPT species and two methods of application (surface versus incorporation). There was an interaction of pruning quality and method of pruning application on N recovery and maize grain yield. For most MPT species used, incorporated prunings gave high nitrogen recovery compared to surface applied prunings. In order to achieve synchrony between N supply from prunings and N demand by a maize crop, relatively large amounts of N should be released into the soil before peak N demand by the maize crop. This could be achieved by incorporating adequate quantities of high quality prunings such as those ofCajanus cajan andLeucaena leucocephala. Florida Agricultural Experiment Station Journal Series Number R-05489.     

Influence of forbs and shrubs on ruminal fermentation and digesta kinetics in sheep fed a grass hay/straw diet

Four ruminally cannulated, Debouillet wethers were used in a 4×4 latin square experiment to evaluate the influence of a forb mix, a shrub mix and alfalfa (ALF) hay on ruminal fermentation characteristics and digesta kinetics of a basal grass hay/straw (GH/S; 1.04% N) diet. The grass hay was mostly blue grama (Bouteloua gracilis) mixed with barley (Hordeum vulgare) straw. Wethers were fed either the GH/S mix (7030), GH/SALF (295813), GH/Sforbs (265420), or GH/Sshrubs (265420). The forb component consisted of equal parts of scarlet globemallow (Sphaeralcea coccinea) and leatherleaf croton (Croton corymbulosus) while shrubs were composed of equal parts of fourwing saltbush (Atriplex canescens) and mountain mahogany (Cercocarpus montanus). Ruminal pH, ammonia-N, as well as total VFA concentrations and molar proportions of individual VFA did not differ among diets. Particulate passage rates tended (P>0.10) to be greater for diets containing ALF, forbs or shrubs (2.8, 2.9 and 2.8% h^–1) than the GH/S diet (2.5% h^–1). In situ DM and NDF disappearance of a GH/straw (5050) mix did not differ among treatments at most incubation times, except at 96 h when extent of both DM and NDF disappearance were greater (P<0.05) in sheep fed either forbs or shrubs than in those fed other diets.