Pregnancy suppression by an antiserum to the sperm specific lactate dehydrogenase

An antiserum induced against the isozyme of lactate dehydrogenase (LDH-X) that is unique to spermatozoa reduced significantly the number of pregnancies in mice treated at varying times after they had mated. This effect of the antiserum occurred both prior to and following implantation. The fecundity of treated animals appeared to be normal in subsequent matings.     

Carbohydrate dramatically influences immune reactivity of antisera to viral glycoprotein antigens

Analysis of the ability of heteroantisera, monoclonal antibodies, and antibodies to synthetic peptides to react with viral glycoproteins deglycosylated with endoglycosidase F revealed that the reactivities of most of the antibodies to these glycoprotein antigens were influenced by the attached carbohydrate moieties. All heteroantisera prepared in rabbits or goats to either fully glycosylated retroviruses or influenza virus were virtually unreactive toward the viral glycoproteins after carbohydrate removal. Analyses with a panel of monoclonal antibodies to purified Rauscher murine leukemia virus gp70 indicated that the reactivity of most of these antibodies improved while the reactivity of others decreased or remain unchanged after carbohydrate removal. Most of the antibodies to synthetic peptide sequences in the influenza virus hemagglutinin also improved in reactivity after carbohydrate removal. These data indicate that carbohydrate side chains on viral glycoproteins influence the immune response to these antigens, and the more native the glycoprotein immunogen, the more dramatic the carbohydrate influence. Thus the immune response to these glycoproteins is not simply a function of the immunogenicity of certain domains over others but rather is a direct measure of carbohydrate influences on the host's perception of the foreign antigen.     

Tumor-specific antigens detected by inhibition of macrophage migration

Tumor-specific antigens of a guinea pig hepatoma induced by diethylnitrosamine were detected by the inhibition of migration of specifically sensitized macrophages from capillary tubes, and by the local passive transfer of delayed skin hypersensitivity and the suppression of growth of intradermally same injected tumor.     

Soluble membrane antigens of lip and cervical carcinomas: reactivity with antibody for herpesvirus nonvirion antigens

With the use of antibody for herpesvirus nonvirion antigens (not structural components of the virus) complement fixing reactivity has been shown for soluble membrane antigens separated from lip and cervical carcinomas but not for similar extracts from normal vaginal tissue or intestinal carcinoma. Neither the serum obtained from the guinea pig before hyperimmunization with the herpesvirus nonvirion antigen nor the antiserum of guinea pigs immunized with comparable uninfected cell extracts reacted with these tumor soluble membrane antigens. Since the above soluble membrane antigens could be specific markers for the presence of virus genome within the tumor cells, the findings could support an etiological role of herpesvirus in selected human malignancies.     

Simian virus 40: replication in the presence of specific antiserum and adenovirus 4

Twelve consecuttive passages of simian virus 40, made in the presence of adenovirus 4 and antiserum to simian virus 40, indicated that simian virus 40 would multiply almost indefinitely under these conditions. The adenovirus and the simian virus had previously replicated together in the absence of antiserum. These data support the conclusion that the SV-40 genome is incorporated within the protein coats of adenovirus 4.     

Differential reactivity of human serums with early antigens induced by Epstein-Barr virus

Inoculation of 64-10 or Raji cultures with Epstein-Barr virus derived from the HRI-K clone of the P3J Burkitt's lymphoma line caused abortive infections in most of the lymphoblastoid cells with synthesis of "early antigens" but few, if any, capsids. Antibodies to early antigens were detected by indirect immunofluorescence in serums of many patients with infectious mononucleosis, Burkitt's lymphoma, or nasopharyngeal carcinoma. These antibodies were rarely present in other serums even though some of them showed high titers of antibodies to Epstein-Barr virus when assayed on EB3 Burkitt tumor cells; they also prevented synthesis of early antigens, provided the serums were mixed with the virus prior to inoculation. Antibodies to early antigens possibly reflect current or recent disease processes that are associated with the virus.     

猪CD40L基因的原核表达及特异性多克隆抗体制备

从猪外周血淋巴细胞中克隆猪CD40L基因,构建表达载体pET-CD40L,转化大肠杆菌BL21(DE),表达获得相对分子质量约为48x103的重组融合蛋白.以纯化的重组蛋白免疫新西兰兔,获得抗猪CD40L的特异性抗体.间接ELISA检测到多克隆抗体效价达到1:12 800,Western-blot检测结果表明,得到的抗体既可与纯化的CD40L蛋白特异性结合,又可与表达猪CD40L的重组杆状病毒特异性结合.     

Repression of colony formation reversed by antiserum to mouse thymocytes

Marrow cells derived from C57BL/6 mice form many fewer splenic colonies in irradiated C57BL/6 x C3H F1 hybrid recipients than in irradiated C57BL/6 recipients (repression of colony formation). This effect is reversed by treatment of the hybrid recipients with active antiserum to mouse thymocytes. The repression phenomenon cannot readily be explained in immunological terms; hence the effect of the antilymphocyte serum on this phenomenon may not result from immunosuppression in the usual sense.     

Prevention of fungal infection of plants by specific inhibition of cutinase

Specific antibodies prepared against cutinase from Fusarium solani pisi and diisopropylfluorophosphate, a potent inhibitor of this enzyme, prevented infection of the host (pea epicotyl) by this organism, without affecting the viability of the spores. This finding shows that enzymatic penetration of cuticle is involved in pathogenesis.     

侵染观赏百合的黄瓜花叶病毒提纯和抗血清制备

采用PEG沉淀并结合差速离心技术,获得了精提纯的侵染观赏百合的黄瓜花叶病毒(CMV-Li).提纯病毒具有典型的核蛋白吸收峰曲线.OD260 nm/OD280 nm=1.236.将提纯病毒免疫兔子制备得CMV-Li抗血清.经微量沉淀法和间接ELISA法测定,其效价分别为1∶1024、1∶4096.两种方法相比,间接ELISA具有灵敏度高、特异反应强等优点.     

Relationship of blood type to histocompatibility in chickens

Evidence shows that the B blood group locus in chickens, which controls red cell antigens, is associated with tolerance of skin homografts. Three other blood group loci studied did not show this effect.     

Thyrotropin-releasing hormone in specific nuclei of rat brain

The regional distribution of thyrotropin-releasing hormone (TRH) in rat brain was studied. The greatest concentration of TRH was found in the median eminence. High concentrations were also found in several hypothalamic nuclei. Outside the hypothalamus, relatively large amounts of TRH were found in the septal and preoptic areas.     

猪繁殖与呼吸综合征病毒NJ-a株ORF6基因的表达及特异性抗体的制备

根据GenBank公布的猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)VR2332 株的核酸序列设计并合成1对特异性引物,用RT-PCR方法扩增PRRSV NJ-a株ORF6基因,将其连接入pMD18-T载体,经测序后克隆入原核表达载体pET-32a( )上,构建原核表达载体pET-ORF6.阳性质粒转化感受态大肠杆菌BL21(DE3),经异醛-β-D-硫代半乳糖苷(IPTG)诱导,PRRSV ORF6基因获得表达.以纯化的重组蛋白为抗原免疫家兔3次,获得抗PRRSV NJ-a株M蛋白的特异抗体.经Western-blotting证明,该多克隆抗体既能与原核表达的重组M蛋白发生反应,又能与PRRSV发生特异性反应;间接免疫荧光结果表明,获得的多克隆抗体与表达PRRSV M蛋白的293T细胞及感染PRRSV的Marc-145细胞发生反应.兔抗PRRSV NJ-a株M蛋白特异性抗体的获得,为进一步研究PRRSV M蛋白的表达和功能奠定了基础.     

Leptin通过JAK-STAT3通路抑制原代大鼠脂肪细胞perilipin mRNA的表达

【目的】探讨瘦素(Leptin)影响大鼠脂肪细胞perilipin、ADRP和TIP47 mRNA表达的分子机理。【方法】以体外培养的原代大鼠脂肪细胞为研究对象,用50 ng/mL Leptin及Leptin与JAK-STAT3通路抑制剂(AG490)、MEK通路抑制剂(U0126)、PPARgamma激活剂(罗格列酮(Ros))联合处理原代大鼠脂肪细胞3 h,提取总RNA,半定量(Semi-quantitative,SQ)RT-PCR法检测PPAR gamma、perilipin、ADRP和TIP47 mRNA的表达情况。【结果】阻断JAK-STAT3通路,可以减弱50 ng/mL Leptin对原代大鼠脂肪细胞perilipin mRNA表达的抑制作用(P<0.05),但加剧了Leptin对大鼠脂肪细胞ADRP和TIP47 mRNA表达的抑制(P<0.05);阻断MEK通路可以加剧Leptin对大鼠脂肪细胞perilipin、ADRP和PPAR gamma mRNA表达的抑制作用(P<0.05)。【结论】Leptin通过JAK-STAT3通路抑制原代大鼠脂肪细胞perilipin mRNA的表达。     

Antigenic changes in lymph-node cells after administration of antiserum to thymus cells

Mice of the RIII and C57BL strains were treated with rabbit antiserum to thymus (ATS), and cells of their lymph nodes were analyzed serologi-cally at intervals after treatment. While lymph-node cells of untreated mice were sensitive to the cytotoxic effect of isoantibodies against the theta antigen, lymph-node cells of ATS-treated mice showed a significantly reduced sensitivity. Three days after ATS treatment lymph-node cells of most mice were completely refractory to the cytotoxic effect of theta antibodies. Administration of normal rabbit serum elicited only a slight reduction of the sensitivity of lymph-node cells to the cytotoxic effect of theta antibodies. The results support the hypothesis that ATS treatment selectively affects a population of thymus-dependent circulating lymphocytes.     

贵州竹乡乌骨鸡Ghrelin抗血清的制备

Ghrelin是1999年发现的脑肠肽,既有强烈的促进生长激素释放功能,又有调节能量平衡、影响消化道激素分泌、促进摄食等多种功能[1-2].Ghrelin前体经过切割后形成成熟肽.Ghrelin主要由胃黏膜分泌,小肠、大肠、胰腺、肾、免疫系统、胎盘、垂体、睾丸、卵巢以及下丘脑等组织处有少量分泌[3],与人类的多种疾病有关[4].     

L-asparaginase: inhibition of early mitosis in regenerating rat liver

L-Asparaginase in agouti serum and in extracts from Escherichia coli inhibits the early wave of mitosis occurring in rat liver approximately 30 hours after hepatectomy, but even with continued treatment of the animal the later wave at 50 hours is not inhibited. This result differs from the permanent inhibition of growth which asparaginase causes in various tumors.     

芽孢杆菌BJ-6抗血清制备及其ELISA检测条件初探

芽孢杆菌BJ-6分离于杏树根部土壤,对多种植物病原真菌有抑菌活性,试验制备其抗血清,明确ELISA反应条件,用于相似抗原性生防茵的筛选.分别以芽孢杆菌BJ-6的抗茵蛋白和茵体研磨物为免疫抗原,制备出两种抗血清,采用间接ELISA方法测定发现:其效价分别为1:3200和1:6400;所购辣根过氧化物酶标记羊抗兔抗体效价为1:7000;待测抗原可在37℃3 h、4℃18 h+37℃3 h、37℃3 h+4℃18 h和4℃18h条件下包被:初步测定两种抗血清具有种的特异性.