"雏痢净"对人工感染雏鸡白痢的疗效研究

对人工感染鸡白痢的艾维茵雏鸡,采用"雏痢净"按高(0.5/只)、中(0.4/只)和低(0.3/只)三个剂量混饲治疗,用2%甲磺酸培氟沙星(药物对照组)、感染不用药(阳性对照组)、不感染不用药(阴性对照组)作为对照.结果"雏痢净"高、中、低剂量组与2%甲磺酸培氟沙星组对雏鸡白痢均有较高的疗效,其中"雏痢净"高、中剂量组与2%甲磺酸培氟沙星组的治疗效果最好,但三者差异不显著(P＞0.05);"雏痢净"各组与2%甲磺酸培氟沙星组的相对增重率极显著高于感染不用药组(p0.01).表明"雏痢净"高、中剂量用药后症状明显减轻且渐恢复,体重也明显增加,其疗效与2%甲磺酸培氟沙星相当.     

"雏痢净"对人工感染雏鸡白痢的疗效研究

对人工感染鸡白痢的艾维茵雏鸡,采用“雏痢净”按高(0.5g/只)、中(0.4g/只)和低(0.3g/只)三个剂量混饲治疗,用2%甲磺酸培氟沙星(药物对照组)、感染不用药(阳性对照组)、不感染不用药(阴性对照组)作为对照。结果:“雏痢净”高、中、低剂量组与2%甲磺酸培氟沙星组对雏鸡白痢均有较高的疗效,其中“雏痢净”高、中剂量组与2%甲磺酸培氟沙星组的治疗效果最好,但三者差异不显著(P>0.05);“雏痢净”各组与2%甲磺酸培氟沙星组的相对增重率极显著高于感染不用药组(P<0.01)。表明:“雏痢净”高、中剂量用药后症状明显减轻且渐恢复,体重也明显增加,其疗效与2%甲磺酸培氟沙星相当。     

"雏痢净"对人工感染雏鸡白痢的疗效研究

对人工感染鸡白痢的雏鸡,采用“雏痢净”按高(0.5g/只)、中(0.4g/只)和低(0.3g/只)3个剂量混饲治疗,用2%甲磺酸培氟沙星(药物对照组)、阳性对照组(感染不用药)、阴性对照组(不感染不用药)作为对照。结果:“雏痢净”高、中、低剂量组、甲磺酸培氟沙星组与感染不用药组相比,死亡率极显著降低(P<0.01),“雏痢净”高、中剂量组、药物对照组的治愈率差异不显著(P>0.05);“雏痢净”各组对鸡的相对增重率极显著高于感染不用药组(P<0.01),但与药物对照组相比差异不显著(P>0.05)。     

雏痢净对人工感染雏鸡白痢的疗效观察

雏鸡白痢是由鸡白痢沙门氏菌引起的鸡和火鸡等禽类的传染病。雏鸡患病呈急性败血性经过，以白痢为主要症状，在成鸡多呈慢性经过或无症状感染。雏鸡白痢一般发生在3周龄内的雏鸡，发病率和死亡率都较高，是阻碍养鸡业发展的重要疾病之一。雏痢净是一种纯中药复方制剂，主要用于防治雏鸡白痢，为了客观评价其对雏鸡白痢的防治效果，特组织进行了本次雏鸡人工感染鸡白痢沙门氏菌病的治疗试验，报道如下。     

925雏痢康防治雏鸡白痢的临床试验报告

本试验应用９２５雏痢康分１０批对１１７９６只伊萨褐及罗曼雏鸡进行了白痢防治试验和临床抗药性观察。结果１０日龄内雏鸡成活率平均为９６．１％；经临床反复试验证实，该工在一个鸡场连续应用一年半之后，效果仍然可靠，未见耐药现象。再且其它药物防治慢性呼吸道病和球虫病。收效显著，未出现交叉耐药现象。     

925雏痢康预防雏鸡白痢的试验

９２５雏痢康系采用中西药物经科学配制的新型兽药,经与吡哌酸、纯中药复方制剂、土霉素预防雏鸡白痢的对比试验结果显示,各组雏鸡１０日龄时成活率分别为９７．５％、８７．７％、８４．６％和３１．８％;重复扩大试验１２批共预防雏鸡１４５９４只,１０日龄时平均成活率为９６．６％。试验证明,该药不仅具有良好的预防效果,而且在连续使用的情况下,病原菌不易产生耐药性,是防治雏鸡白痢的理想药物。     

"雏痢清"对雏鸡人工诱发大肠杆菌感染的防治效果观察

采用“雏痢清”对雏鸡人工诱发大肠杆菌感染的预防和治疗效果试验。每一试验均分为健康对照组、感染对照组,“雏痢清”高、中、低剂量组和药物对照等6组,每组30只三周龄石歧杂雏鸡,试验前后每只鸡分别称重,试验期2周。预防试验:“雏痢清”在人工诱发大肠杆菌感染前1天按0.1％(C3组)、0.2％(C2组)、0.4％(C1组)添加饮水中,让雏鸡自由饮用5天(雏鸡感染后连续2—3天饮水和吃料均停止),结果痊愈率分别为16.7％(5/30)、63.3％(19/30)、93.3％(28/30);感染对照(B1)组10.0％(3/30);药物对照组(C4)为56.7％(17/30)。相对增重率分别为:37.63％、93.71％、104.32％、82.99％和64.89％。治疗试验:当人工诱发大肠杆菌3小时后,试验组按抗茵药含量5 mg/kg(D3组)、10 mg/kg(D2组)、20 mg/kg(D1组)人工灌服药物3天,自由饮用2天,结果其痊愈率分别为40.0％(12/30)、77.0％(23/30)、100.0％(30/30);感染对照(B2)组36.7％(11/30);药物对照组(D4)为93.3％(28/30)。相对增重率分别为74.19％、70.45％、69.31％、69.79％和54.36％。以上试验显示:“雏痢清”与剂量相同的抗菌药对雏鸡人工诱发大肠杆菌感染痊愈率方面比较,无显著性差异(P>0.05);在增重方面比较,无论是预防还是治疗,“雏痢清”显著比抗菌药高(P<0.05)。     

痢康灵微生态制剂对雏鸡白痢的治疗试验

微生态制剂的治疗作用报告不一 ,相差很大 ,为了搞清本室自制的微生态制剂痢康灵对鸡白痢的治疗作用及为了现场治疗提供治疗时机 ,进行了本试验。1　材料与方法1 .1　痢康灵微生态制剂 :含菌量 1 0 10 ＣＦＵ ｇ ,为本室自制 ,治疗用量 0 .2ｇ 羽 ,每日 2次 ,连用 3天。1 .2　试验动物 :选 8日龄健康蛋用公雏 75羽 ,分为 5组。时间 :1 998年 4月1 .3　鸡白痢沙门氏菌强毒 :购自中国兽药监察所 ,代号 :Ｃ79— 14 。1 .4　试验方法 :将试验鸡前 4组用鸡白痢沙门氏菌Ｃ79— 14 口服攻击 ,( 0 .2ｍｌ含 6× 1 0 6ＣＦＵ ｇ)第 5组不攻毒作对照组…     

泻痢安糖糊对人工感染雏鸡鸡白痢沙门氏菌的治疗实验

以鸡白痢沙门氏菌为菌种,对２～３日龄雏鸡进行了人工感染致病的分组药物治疗比较试验。取２１０只雏鸡随机分成７组,除阴性对照组外,每组都接种鸡白痢沙门氏菌,每只鸡的接种量按最小致死量给予。阳性对照组不给药治疗,其中５组分别用泻痢安糖糊高剂量、中剂量、低剂量、中药鸡痢灵和西药可溶性氟哌酸给予治疗,连续用药４ｄ,停药观察７ｄ。结果表明,泻痢安中剂量组的存活率达９０％,即泻痢安中剂量对雏鸡鸡白痢的治疗效果可列各组之首。     

增效泻痢宁对雏鸡白痢的防治试验报告

我们在用增效泻痢宁胶囊对鸡白痢杆菌进行了体外抑菌试验的基础上,对雏鸡白痢进行了预防和治疗试验,并与痢特灵进行了预防比较试验,收到了较好的效果。试验结果表明该药预防鸡白痢具有高效、速效、低     

Reduced mucosal injury of SPF chickens by mast cell stabilization after infection with very virulent infectious bursal disease virus

Recent studies here have demonstrated that increased mast cell populations and tryptase activity contribute to lesion formation in regions of immune organs in special-pathogen-free chickens after infection with very virulent infectious bursal disease virus (vvIBDV). Mast cells and their mediators have been implicated in acute inflammatory injury after vvIBDV infection, but their precise role in this process remains elusive. In this study, the role of mast cells in the vvIBDV infection process was examined using ketotifen, a mast cell membrane stabilizer. On days 1, 2, and 3 postinfection, the bursa of Fabricius (BFs) were collected to quantify mast cells, tryptase and histamine contents by cytochemistry, immunohistochemistry and fluorospectrophotometry analyses, respectively. The results showed that the mast cell populations, tryptase expression, and histamine released increased significantly in the BFs (p < 0.01) of infected birds compared to controls, and acute inflammatory responses were observed in the former. In contrast, in infected chickens pretreated with ketotifen, mast cells, tryptase, and histamine were markedly decreased (p < 0.01) and probably as a result, the BFs remitted significantly. The overall results suggest that mast cells are positively involved in BF injury induced by vvIBDV infection. Inhibition of mast cell degranulation and concurrent mediator release may represent a novel strategy to modulate this process. This study, thus, advances the understanding of the acute inflammatory injury mechanisms triggered by vvIBDV infection and the contribution of mast cell activity in this process.     

中药饲料添加剂提高鸡产蛋性能试验

用黄芪、当归、益母草等 12味中药组成的中药饲料添加剂 ,分别按 0 .5 % (A组 )和 1% (B组 )的比例添加于饲料中 ,连喂 2 0 d,与不添加组 (C组 )作对比试验 ,连续观察统计产蛋率等指标 4 0 d,结果发现 ,A、B二组鸡产蛋率均比 C组高 ,差异极显著 (P<0 .0 1) ,说明该添加剂有良好饲用效果。     

Molecular characterization of two Bangladeshi infectious bursal disease virus isolates using the hypervariable sequence of VP2 as a genetic marker

Two Bangladeshi infectious bursal disease virus (IBDV) isolates collected in 2007, termed GB1 and GB3, were subjected to comparative sequencing and phylogenetic analyses. Sequence analysis of a 474-bp hypervariable region in the VP2 gene revealed that among four major amino acid substitutions observed in the strains, two were unique to GB1 and GB3 (Ser217Leu and Ala270Thr) while one substitution was only found in GB1 (Asn299Ser). Among IBDVs from Bangladesh including GB1 and GB3, the rate of identity and homology was around 97~99%. The amino acid sequences of GB1 and GB3 differ from those of previous Bangladeshi IBDV isolates and contain amino acid substitutions Pro222Ala and Asn299Ser (in GB3 only). Phylogenetic analysis revealed that GB1 and GB3 are grouped with other very virulent IBDVs of European and American origin in contrast to two previously isolated Bangladeshi IBDV strains (GenBank accession Nos. {"type":"entrez-nucleotide","attrs":{"text":"AF362776","term_id":"14582985","term_text":"AF362776"}}AF362776 and {"type":"entrez-nucleotide","attrs":{"text":"AF260317","term_id":"13957666","term_text":"AF260317"}}AF260317), which belong to the Asian group. It was concluded that GB1 and GB3 belong to a very virulent group of IBDVs. However, amino acid sequences of GB1 and GB3 differ from those of the other Bangladeshi IBDVs by one or two amino acids encoded in the hypervariable region of the VP2 gene.     

Dexamethasone reduces infectious bursal disease mortality in chickens

Very virulent infectious bursal disease virus (vvIBDV) causes high mortality in chickens but measures to reduce the mortality have not been explored. Chickens (8–9 weeks) were treated with 3 agents before and during vvIBDV inoculation. Dexamethasone treatment reduced the mortality of infected chickens (40.7% vs. 3.7%; p < 0.001), but treatment with aspirin or vitamin E plus selenium did not affect the mortality. The bursa of Fabricius appeared to have shrunk in both dead and surviving chickens (p < 0.01). The results indicate that dexamethasone can reduce mortality in vvIBDV-infected chickens and may provide therapeutic clues for saving individual birds infected by the virus.     

头孢噻呋对实验性感染鸡白痢沙门氏菌病的药效学研究

研究了头孢噻呋对实验性感染鸡白痢沙门氏菌的防治效果。结果显示，头孢噻呋体内疗效良好，治疗组与预防组的治愈率、有效率、相对增重率均不同程度高于感染对照组（P〈0.05或P〈0.01）；头孢噻呋的治疗效果高剂量组与中剂量组无显著差异（P〉0．05），高剂量组与低剂量组差异显著（P〈0．05）；头孢噻呋的中剂量组与头孢曲松、氨苄西林组的疗效相比均差异显著（P〈0．05或P〈0．01）。实验结果表明，头孢噻呋按0．1mg／只的剂量肌肉注射，每日一次，可对鸡白痢沙门氏菌病起到有效的防治效果。     

Inductive expression of the NOD1 signalling pathway in chickens infected with Salmonella pullorum

1. The aim of this study was to describe the role of Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) receptor signalling in chicken.

2. Tissue-specific expression analysis of NOD1, receptor-interacting serine-threonine kinase 2 (RIPK2), nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase 11 (MAPK11 or p38) by quantitative real-time PCR (qRT-PCR) revealed their wide distribution in various organs and tissues.

3. Salmonella pullorum infection activated NOD1 receptor signalling in vivo and in vitro, resulting in significant induction of downstream signalling molecules RIPK2, NF-κB/p65, MAPK11/p38 and the effector molecules IL-1b and IL-8.

4. Activation of NOD1 by its agonist bacterial γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP) in HD11 cells induced the adapter molecular RIPK2 and activated the NF-κB/p65 and MAPK11/p38 pathways, resulting in an increase in IL-8 but not IL-1β. Additionally, inhibition of NOD1 using NOD1-shRNA resulted in downregulation of RIPK2, MAPK11 and IL-8, while NF-κB/p65 and IL-1β were unaltered.

5. These results highlight the important role of NOD1 receptors in eliciting the innate immune response following pathogenic invasion in chicken.     

部分省市鸡白痢和鸡伤寒的单抗阻断ELISA血清学调查

采用单抗阻断ELISA法对我国部分省市不同品种、不同类型的鸡群进行了鸡白痢、鸡伤寒的血清流行病学调查。结果，在2309份鸡血清样品中检出阳性359份，阳性率为15．5％，不同鸡场鸡群感染率悬殊较大，从0到54．5％不等。表明，我国鸡群中鸡白痢沙门菌、鸡伤寒沙门菌的流行呈现多样化。     

中药复方散剂对肉鸡生长及鸡新城疫灭活油苗免疫效果的影响

将玉屏风散、十全大补汤、六味地黄丸三个中药复方散剂分别按1%剂量添加到科宝快大型白鸡日粮中, 于雏鸡14日龄, 按0.5 ml/只剂量肩部肌肉注射鸡新城疫灭活油乳剂疫苗, 即日起饲喂上述饲料, 并按40 mg/kg剂量胸肌注射环磷酰胺,每天1次, 连续3天; 免疫后第10天、 20天、 30天采血检测鸡新城疫HI抗体效价,观测鸡增重情况,免疫后第15天、 25天检测血清总蛋白含量.实验结果表明,玉屏风散、六味地黄丸能显著提高鸡新城疫HI抗体效价(P<0.05),十全大补汤的作用不明显;玉屏风散、十全大补汤能显著增加肉鸡体重(P<0.05),六味地黄丸的作用不明显,实验组鸡死亡率均降低;玉屏风散、十全大补汤能显著提高血清总蛋白含量(P<0.05),六味地黄丸的作用不明显.