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1.
Hereford and Hereford X Angus cows (n = 31) were utilized to determine the effects of heat stress on early embryonic development and survival. After acclimation to handling, cows were cannulated via the jugular vein on d 7 and assigned to either a control (C) chamber environment of 22 C, 35% relative humidity (RH) or one of two heat stress treatments. Ambient temperature was maintained at 37 C for 12 h followed by a decrease to 33 C for the remainder of the day in both treatment groups. Relative humidity was maintained at 27% in treatment 1 (TRT 1) and 38% in treatment 2 (TRT 2). On d 8 to 16, daily measurements of respiration rate (RES), rectal temperature (REC) and water intake were taken along with samples of blood, which were analyzed for hematocrit (HEM) and plasma concentration of protein (PP), progesterone (P4), estradiol-17 beta (E2), thyroxine (T4) and glucose (GLU). The uterus was recovered and flushed with saline on d 17 to recover the conceptus and uterine contents. Conceptus (if present) and corpus luteum (CL) wet weight were determined. Cows subjected to TRT 2 had increased RES and REC (P less than .01), while HEM was decreased (P less than .05) compared with C cows. Plasma T4 concentration was decreased (P less than .10) in TRT 2 compared with TRT 1 and cows, while P4 concentration were not significantly different. Corpora lutea wet weights were reduced (P less than .10) in heat-stressed cows vs C cows.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
2.
本文运用交感神经阻断剂6-OHDA(100 mg/kg体重)腹腔注射昆明系小鼠,研究交感神经对妊娠早期外周血清性激素水平的影响.结果显示:交感神经损毁后,妊娠早期外周血清中雌二醇浓度着床前(E3)升高明显,着床期下降;血清中孕酮浓度着床前升高,着床后降低.结论:交感神经通过调控妊娠早期外周血性激素的生理水平,从而维持妊娠的发生,促进妊娠早期胚胎发育. 相似文献
3.
This review highlights the role of the oviduct in early embryo development, which has to fulfil many aligned and well-tuned tasks during early embryogenesis. The oviductal lining is subjected to dynamic changes to timely accomplish gamete transport, fertilization and embryo development and to deliver a competent and healthy conceptus to the endometrium which can implant and develop to term. Although knowledge about the role of the oviduct is limited, we know that embryos are very sensitive to the environment in which they develop. The success of in vitro embryo production techniques demonstrates that it is possible to bypass the oviduct during early development and, to a certain extent, replicate the conditions in vitro. However, comparative studies show that embryos developed in vivo are superior to their in vitro produced counterparts, underlining our relatively poor knowledge of the biology of the oviduct. Oviduct activity is orchestrated by various factors, depending on cyclic dynamics, which crucially affect the success of tubal transfer and/or (re-)collection of embryos in embryo transfer studies. This paper reviews data which demonstrate that in vivo culture of embryos in the bovine oviduct is a useful tool for the assessment of embryos developed under various conditions (e.g. superovulation vs single ovulation, lactating dairy cows vs non-lactating cows). It is concluded that more work in the field of early embryo development within the oviduct would contribute to improved ART protocols leading to healthy pregnancies and offspring. 相似文献
4.
卵母细胞胞质中存在着大量的母源性信息,正常受精卵的发育启动和早期卵裂主要由这些母源性信息所控制。为了评估卵胞质含量对早期胚胎发育的影响,以假显微注射兔原核胚为对照,采用显微注射技术将兔原核期胚胎去除或增加一定量的细胞质:试验一,增加5%和20%的细胞质;试验二,移入5%小鼠MⅡ期卵母细胞胞质。胚胎经显微操作后进行R1/R2序贯培养。结果显示,(1)增量5%组胚胎的2细胞胚(72.00%)、8细胞胚(60.00%)、桑椹胚(58.00%)和囊胚(54.00%)的发育率均显著(P〈0.05)高于增量20%组胚胎(分别为47.62%、35.71%、33.33%和30.95%),但与对照组之间无显著差异(P〉0.05);囊胚细胞数在2个增量组和对照组3者之间也无显著差异(P〉0.05)。(2)将5%小鼠MⅡ期卵胞质移入兔原核胚后,各阶段胚胎发育率和囊胚细胞数与增量5%组、对照组相比均无显著差异;早期胚胎经PCR检测,在2细胞胚(5/5)、8细胞胚(5/5)和桑椹胚(5/5)均全部检测到了供体小鼠mtDNA的D—loop区3’端片段,而在囊胚却仅有1枚(1/5)能够检测到。结果表明,增加少量细胞质不会对兔原核胚的体外发育造成显著影响,但胚胎发育率随着细胞质体积的增加有下降趋势,而异种卵胞质对兔原核胚的发育没有明显影响,在囊胚期虽仍能检测到异种卵胞质中mtDNA的存在,但异种mtDNA会随胚胎发育而逐渐减少。 相似文献
5.
为了研究交感神经对妊娠的调控作用,给小鼠连续5 d腹腔注射交感神经化学损毁剂——6-OHDA(100 mg/kg),将交感神经化学性损毁,然后通过阴道涂片法和显微解剖的方法,对交感神经损毁后小鼠发情周期的变化、排卵的数量和妊娠发生后胚胎数量及大小进行了观察。结果表明:交感神经化学损毁后发情周期和排卵的数量没有明显的变化,但是胚胎的数量明显减少,小鼠胚胎数量在妊娠第7天比对照组同期减少了64.4%,在妊娠第9天比对照组同期减少了69.9%;对胚胎的大小进行测量时发现,交感神经损毁组在妊娠第7天与对照组同期相比,长径减小了0.51 mm,短径减小了0.56 mm;妊娠第9天时,交感神经损毁组与对照组同期相比,长径减小了0.37 mm,短径减小了0.47 mm,差异显著(P<0.05)。说明交感神经主要影响胚胎的早期发育,对发情周期和排卵没有明显的影响。 相似文献
6.
The development of cleavage stage preimplantation embryos is disrupted by exposure to heat shock, such as high temperatures in the summer season. In this study, we investigated whether addition of anthocyanins, which are strong scavengers of reactive oxygen species (ROS), improves development and intracellular redox status of heat-exposed bovine preimplantation embryos by reduction of heat shock-derived oxidative stress. After in vitro fertilization (IVF), embryos were cultured at 38.5 C through Day 8 (Day 0=day of IVF) with 0, 0.1, 1 and 10 microg/ml anthocyanins (non-heat-shocked group). On Day 2, embryos were cultured at 41.5 C for 6 h with 0, 0.1, 1 and 10 microg/ml anthocyanins followed by culture at 38.5 C until Day 8 (HS group). After exposure to heat shock, the intracellular ROS and glutathione (GSH) contents of individual embryos were measured in the non-heat-shocked and HS groups using fluorescent probes. On Day 8, the blastocysts formation rates of the embryos and total cell numbers of blastocysts were evaluated. Embryos exposed to heat shock without anthocyanins showed a significant decrease in blastocyst formation rate and GSH content (P<0.05) and an increase in intracellular ROS (P<0.05) compared with non-heat-shocked embryos. In contrast, addition of 0.1 microg/ml anthocyanins significantly (P<0.05) improved the blastocyst formation rate of the heat-shocked embryos. Addition of any dose of anthocyanins produced a significant decrease in the ROS levels (P<0.05) and tended to increase the GSH levels under heat-shock conditions. However, addition of higher concentrations (1 and 10 microg/ml) of anthocyanins to the culture media under heat shock did not improve the development of embryos. These results indicate that anthocyanins maintain the intracellular redox balance of heat-shocked bovine embryos by reducing intracellular oxidative stress and increasing the GSH levels. Thus, alterations of the redox state using natural antioxidative polyphenols is a useful approach for reducing heat shock-derived oxidative stress. 相似文献
7.
Nutrition has been shown to influence several reproductive functions, including hormone production, oocyte competence and fertilization, and early embryonic development. To determine the effects of maternal diet on in vitro fertilization (IVF) and early embryonic development, ewes (n = 18; 47.0 +/- 1.5 kg of initial BW) were divided into control and underfed (60% of control) nutritional planes for 8 wk before oocyte collection. Pelleted diets containing 2.4 Mcal of ME/kg and 13% CP (DM basis) were fed once daily. During the first 4-wk acclimation phase, control and underfed ewes were fed 1,000 and 600 g/d, respectively. From wk 4 to 8, control (adequate) ewes were fed to maintain BW and offered 720 g/d, whereas underfed ewes received 432 g/d (60% restricted). Synchronization of estrus was performed using progestagen sponges for 14 d. Follicular development was induced by twice daily injections of FSH on d 13 (5 units/injection) and 14 (4 units/injection) of the estrous cycle. Oocytes were collected from all visible follicles on d 15 of the estrous cycle. After IVF, the proportion of developing embryos was evaluated throughout an 8-d culture period. Under-nutrition decreased (P < 0.006) the rate of cleavage, number of blastocysts per ewe, and rate of blastocyst formation (from 79 to 64%; from 3.3 to 0.8; and from 31 to 8%, respectively). However, the number of visible follicles, total number of oocytes, number of healthy oocytes, percentage of healthy oocytes, number of cleaved oocytes, and morula formation per ewe were similar for control and underfed ewes. These data indicate that undernutrition of donor ewes, resulting in lower BW and BCS, has a negative effect on oocyte quality, which results in lower rates of cleavage and blastocyst formation. 相似文献
8.
Methylglyoxal (MG) is a precursor for the generation of endogenous advanced glycation end-products involved in various diseases, including infertility. The present study evaluated the motility and developmental competence after in vitro fertilization of mouse sperm which were exposed to MG in the capacitation medium for 1.5 h. Sperm motility was analyzed using an SQA-V automated sperm quality analyzer. Intracellular reactive oxygen species (ROS), membrane integrity, mitochondrial membrane potential, and DNA damage were assessed using flow cytometry. The matured oocytes were inseminated with MG-exposed sperm, and subsequently, the fertilization and embryonic development in vitro were evaluated in vitro. The exposure of sperm to MG did not considerably affect the swim-up of sperm but resulted in a deteriorated sperm motility in a concentration-dependent manner, which was associated with a decreased mitochondrial activity. However, these effects was not accompanied by obvious ROS accumulation or DNA damage. Furthermore, MG diminished the fertilization rate and developmental competence, even after normal fertilization. Collectively, a short-term exposure to MG during sperm capacitation had a critical impact on sperm motility and subsequent embryonic development after fertilization. Considering that sperm would remain in vivo for up to 3 days until fertilization, our findings suggest that sperm can be affected by MG in the female reproductive organs, which may be associated with infertility. 相似文献
9.
This experiment was conducted to investigate the effects of dietary supplemental L-ascorbic acid (AA) and folic acid (FA) on broiler chickens under heat stress (HS) conditions when supplemented either alone or in combination. For this aim, the effect of these supplements on the broilers’ growth performance, some blood parameters, antioxidant, and immune status were evaluated. Hatchling Cobb-500 broilers (total = 240) were fed either corn and soybean meal-based diet (control group) or basal diet supplemented with 200 mg AA/kg diet, 1.5 mg FA/kg diet, or 200 mg AA plus 1.5 mg FA/kg diet, for 35 days during the summer months (n = 6 replicates/group, 10 birds/replicate). The minimum and maximum average temperatures ranged from 84.5 to 96.2 °F, and the relative humidity ranged from 68.5 to 76.5%. Supplemented vitamins either alone and (or) in combination increased (P < 0.01) broilers’ growth performance, thyroid hormones levels, insulin growth factor1, blood hemoglobin, total protein, albumin, globulin, heat shock protein70, total antioxidant capacity, catalase enzyme activity, superoxide dismutase enzyme activity, antibody titer against Newcastle disease virus, and decreased (P < 0.01) heterophil/lymphocytes. The effects (P < 0.01) of the supplemented vitamins on the analyzed parameters were better when they were added in combination. In conclusion, the combination of AA and FA at 200 and 1.5 mg/kg, respectively, increased the broilers’ antioxidant status with coordinated improvement in the growth performance and health status under HS. 相似文献
10.
Many factors affect development of mammalian preimplantation embryos in vitro. It is well known that in vitro development of bovine embryos is highly affected by culture condition including energy source, growth factors, pH or gas environment. Many efforts have been made towards the suitable environments which can successfully support embryo development in vitro. For a rapid growth and differentiation, embryo requires energy by utilizing ATP, NADPH with oxygen molecules. These energy substrates are produced from the electron transport chain in the mitochondria. In addition to energy production, reactive oxygen species (ROS) are also generated as by-product of such energy production system. ROS production is sensitively controlled by the balance of oxidizing and reducing status and affected by several antioxidant enzymes such as superoxide dismutase (SOD), Catalase, glutathione peroxidase (GPx) or low molecular weight thiols such as glutathione (GSH). Imbalance of oxidation and reduction causes production of excess ROS, which causes the developmental arrest, physical DNA damage, apoptosis induction or lipid peroxidation. Environmental oxygen condition during embryo culture also highly affects embryo development as well as intracellular redox balance. Several studies have revealed that regulation of intra- and extra- cellular reducing environment by reducing excess ROS by using antioxidants, reducing oxygen concentration are effective for improving embryo development. Also, recent studies have demonstrated the difference in gene expression affected by oxidative stress. This review briefly summarizes the effects of ROS and the role of redox balance on preimplantation embryos for improving the efficiency of in vitro production of mammalian embryos. 相似文献
11.
Incidence of early embryonic death (EED) and associated changes in serum cortisol, progesterone and plasma ascorbic acid (AA) in transported mares were investigated. Mares were transported for 472 km (9 h) during either d 16 to 22 (T-3 wk, n = 15) or d 32 to 38 (T-5 wk, n = 15) of gestation. Blood samples were drawn from control, nontransported mares (NT-3 wk, NT-5 wk, n = 24) and transported mares pre-trip, midtrip, and at 0, 12, 24, 48 and 72 h post-transport and daily for the next 2 wk. Incidence of EED between transported and nontransported mares was not different (P greater than .05). Serum cortisol in all transported mares increased (P less than .05) relative to pre-trip values at midtrip and 0 h post-transport. Relative to NT mares, serum cortisol was higher (P less than .05) at midtrip in T-3 wk mares and 0 h post-transport in T-5 wk mares. Serum progesterone in all T mares increased (P less than .05) at midtrip relative to pre-trip values and was higher (P less than .05) in T-3 wk mares than in NT-3 wk mares at midtrip and 0 h post-transport. Post-transport decreases (P less than .05) in concentrations of progesterone were observed in mares that aborted. Plasma AA in transported mares increased (P less than .05) at midtrip in T-5 wk mares and decreased (P less than .05) relative to pre-trip values at 24 and 48 h post-transport (T-3 wk and T-5 wk mares, respectively).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
12.
每天在固定时间用不同梯度温度(42℃,37℃和33℃)处理实验组鼠1 h,热应激前后分别记录小鼠的肛温和体重.在应激的第8 d、13 d分别处死受试鼠后分离睾丸、附睾并分别称量其重量,计算睾丸、附睾与体重指数,将睾丸和附睾组织切片后镜检.结果表明,应激组小鼠的肛温显著升高(P<0.05);睾丸和附睾内的精子数量、生精细胞上皮层数显著减少,精母细胞数量减少,且排列混乱,细胞的凋亡数量增多;而睾丸、附睾与体重指数无明显变化. 相似文献
13.
选用16头健康的中国荷斯坦奶牛,随机分为两个处理(夏季组和冬季组),每个处理8个重复,每个重复1头牛。当夏季组奶牛牛舍温湿度指数(THI)〉72的时间连续达到15d,冬季组牛舍THI〈72的时间连续达到15d时,颈静脉采血15mL制备血清,检测血清中内毒素、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性,以及丙二醛(MDA)的含量。结果表明:夏季热应激可导致奶牛血清内毒素、MDA含量极显著升高,SOD和GSH-Px活性极显著和显著(P〈0.05)下降。 相似文献
14.
将420只体质量为(15.32±2.01)g的雌性昆明系小鼠随机分为5个处理组和1个对照组,每组设7个重复,每个重复10只。各处理组分别腹腔按体质量注射10、15、20、25、30mg/kg的醋酸铅溶液,对照组注射等体积的灭菌生理盐水,每隔2d注射并称重1次,共注射10次,期间记录小鼠体质量及临床表现。当小鼠体质量达到25g以上时,分批对各试验组和对照组进行超排处理,腹腔注射10IU马绒毛膜促性腺激素(PMSG),47h后注射10IU人绒毛膜促性腺激素(hCG),并与公鼠合笼。合笼后87~96h内颈椎脱臼处死小鼠,观察卵巢、子宫形态,并统计胚胎数,同时制作卵巢、子宫石蜡切片,观察其病理组织学变化,研究醋酸铅对雌性小鼠卵巢、子宫组织结构及早期胚胎发育的影响。结果显示:(1)当醋酸铅染毒剂量≥20mg/kg时,可明显抑制小鼠体质量的增长,随着染毒剂量的增加,作用时间的延长,小鼠体质量增加明显趋缓,与对照组相比,差异显著或极显著(P0.05,P0.01);(2)染铅组母鼠早期胚胎发育受到显著影响,主要表现为回收胚胎总数以及受精卵发育到桑椹胚和囊胚的总数均显著低于对照组(P0.05),而各染铅组退化胚、延迟胚数和未受精卵总数显著高于对照组(P0.05);(3)染铅组卵巢中的初级卵泡、次级卵泡、成熟卵泡数量明显低于对照组,而原始卵泡、闭锁卵泡数量明显高于对照组。(4)染铅组小鼠卵巢和子宫形态发生明显畸形,当醋酸铅染毒剂量≥20mg/kg时,与对照组相比,差异显著或极显著(P0.05,P0.01)。且上述变化均呈明显的剂量一时间效应。研究结果表明,当醋酸铅暴露剂量≥20mg/kg时,可对小鼠生长发育具有明显抑制作用,同时使母鼠生殖器官卵巢和子宫的结构造成严重损害,并影响其生殖功能与早期胚胎发育。 相似文献
15.
The current study was conducted to investigate the effect of stocking density (SD) on oxidative stress status and mammary gland permeability in early lactating dairy cows. Thirty‐two dairy cows were allocated into 16 blocks, basing on parity, previous milk yield, and body weight, and were then randomly assigned into one of the two treatments as follows: 75% (75SD) and 100% (100SD) SD. The cows were fed with same diet throughout the 8‐week experimental period. The milk yield and milk sample were collected on two consecutive days during the 8‐week experimental period weekly. Plasma samples were collected on fourth and eighth experimental weeks. Raw, energy‐corrected, and 4% fat‐corrected milk yield were significantly higher in 75SD‐cows than that of 100SD‐animals, respectively. The milk somatic cell count was lower in 75SD‐cows than that of 100SD‐animals. The levels of Na +, Na +/K +, bovine serum albumin and plasmin were lower in 75SD‐cows than those of 100SD‐cattle, respectively. The 75SD‐cows had reduced insulin and insulin/glucagon levels but higher prolactin and growth hormone concentrations, compared with those of 100SD‐animals, respectively. In conclusion, compared with low SD‐animals, early lactating cows with higher SD had higher oxidative stress status, which further led to a greater mammary gland permeability. 相似文献
16.
为研究光照周期和限饲对蛋鸡卵巢和输卵管发育及形态学的影响,选用体质健康的10周龄海兰灰蛋鸡240只,采用3×2双因子设计,分别在短光照周期(8L∶16D),中长光照周期(12L∶12D)和长光照周期(16L∶8D)下采取自由采食(对照)和限饲(自由采食量的80%)处理,共计6个组,分别为短光照对照组(Ⅰ)、短光照限饲组(Ⅱ)、中长光照对照组(Ⅲ)、中长光照限饲组(Ⅳ)、长光照对照组(Ⅴ)和长光照限饲组(Ⅵ),每组4个重复,每个重复10只鸡。结果显示:1光照周期显著影响14周(w)卵巢单位面积内卵泡数量(P0.05)及18 w卵巢卵泡直径(P0.05),短光照周期组卵巢单位面积内卵泡数量显著高于中长光照周期组(P0.05);短光照周期的卵巢卵泡直径显著高于中长光照周期和长光照周期组(P0.05);2饲喂水平显著影响14 w卵巢重及18 w卵巢卵泡直径(P0.05),Ⅲ组卵巢重显著高于Ⅳ组(P0.05),3个对照组18 w卵巢卵泡直径分别显著高于其限饲组(P0.05);3光照周期与饲喂水平的互作对18 w卵巢卵泡直径及18 w卵巢单位面积内卵泡数量影响显著(P0.05);43个对照组输卵管皱褶发育均匀,皱褶长度,厚度明显优于其限饲组。 相似文献
17.
作者阐述了热应激对种公牛精液品质的影响及使公牛的促黄体素(LH)、睾酮水平降低,精子生成受阻,精液品质严重下降。热应激所致的精液品质下降需经7~9周才能恢复正常。降温可减缓热应激对种公牛繁殖机能的危害。 相似文献
18.
The effects of viral induced fever and environmental hyperthermia on embryonic development in pregnant rabbits are described. Embryonic development was not disturbed following fever, but there were anomalies in development following hyperthermia. Possible explanations for the different effects of hyperthermia and fever are discussed. 相似文献
19.
As a natural plant‐derived antitoxin, resveratrol possesses several pharmacological activities. This study aimed to evaluate the effects of resveratrol addition on nuclear maturation, oocyte quality during in vitro maturation (IVM) of porcine oocytes and subsequent early embryonic development following somatic cell nuclear transfer (SCNT). Our experiments showed that the treatment of porcine oocytes with 5 µM resveratrol during IVM resulted in the highest rate of the first polar body extrusion. Treatment of oocytes with resveratrol had no influence on cytoskeletal dynamics, whereas it significantly increased glucose uptake ability compared to the control oocytes. Oocytes matured with 5 μM resveratrol displayed significantly lower intracellular reactive oxygen species (ROS) levels and higher relative mRNA expression levels of the genes encoding such antioxidant enzymes as catalase ( CAT) and superoxide dismutase 1 ( SOD1). In addition, resveratrol also prevented onset and progression of programmed cell death in porcine oocytes, which was confirmed by significant upregulation of the anti‐apoptotic B‐cell lymphoma 2 ( BCL‐2) gene and significant downregulation of the pro‐apoptotic BCL2‐associated X ( BAX) gene. Furthermore, the blastocyst rates and the blastocyst cell numbers in cloned embryos derived from the oocytes that had matured in the presence of 5 μM resveratrol were significantly increased. In conclusion, supplementation of IVM medium with 5 μM resveratrol improves the quality of porcine oocytes by protecting them from oxidative damage and apoptosis, which leads to the production of meiotically matured oocytes exhibiting enhanced developmental potential following SCNT. 相似文献
20.
In this study the development of the bovine Fallopian tube was investigated using light microscopic methods. Formation and differentiation of the Müllerian duct were studied in mesonephroi of 16 embryos and fetuses with a crown-rump lengths (CRL) of 0.9-8.4 cm. The funnel field, the rostral beginning of the Müllerian duct was first observed at a CRL of 0.9 cm. It appears as a thickening of the mesothelium on the craniolateral side of the mesonephros. During later development the Müllerian duct emerges by caudal outgrowth from the funnel field. Formation of a common basal lamina surrounding the caudal tips of Müllerian and Wolffian ducts could be observed at all stages up to CRL of 2.7 cm. The mesothelium and the epithelium of the Wolffian duct adjacent to the Müllerian duct showed a modification of epithelium height in all examined stages. Probably the Wolffian duct influences the growth of Müllerian duct by epithelio-mesenchymal interactions. Fetuses from a CRL of 12.0 to 94.0 cm were used for investigation of the prenatal differentiation of the oviductal mucosa. Folding of the oviductal mucosa started at a CRL of 29.0 cm and continued until birth. Individual primary, secondary and tertiary folds are formed in special proliferation zones and epithelium-folding buds. The cellular differentiation of the oviductal epithelium involves the formation of ciliated and secretory cells during different times of prenatal development. Ciliogenesis was first detected at a CRL of 33.0 cm. Active secretory cells could be observed in the oviductal epithelium from a CRL of 64.0 cm onwards. 相似文献
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