我国苜蓿病害研究进展

综述了我国苜蓿主要病害的分布和危害、病原学、发生规律、抗性种质材料的遗传筛选和鉴定及综合防治等方面的主要研究进展,并提出了今后的研究方向和发展目标。     

中国干旱区的湖泊

湖泊作为地表水的重要载体,参与自然系统的水分循环,这在世界干旱地区显得格外突出和重要.应用浏览软件(Google Earth),对2001-2005年中国干旱区湖泊进行判别,并结合有关实地调查资料得知,我国干旱区有大小湖泊近400个,其中,10 km2以上的有29个,10 km2以下的有334个,在我国3大自然地理区域中位居第二.开展干旱区湖泊的研究,对破解干旱区的气候变化和湖泊演变过程、形成特征,以及湖泊水生生物资源的科学开发利用和保护具有重要的理论和现实意义.     

真菌甲硫氨酸生物合成途径研究进展

 甲硫氨酸是蛋白质的重要组分,同时还可通过生成S-腺苷甲硫氨酸（SAM）调控细胞内多种生理过程。本文根据酿酒酵母、粗糙脉孢菌和构巢曲霉3种模式真菌中甲硫氨酸生物合成的最新研究进展,勾画出真菌的甲硫氨酸合成途径,综述了该途径中关键酶的生物学功能,探讨了该途径的调控机制,为病原真菌甲硫氨酸合成的研究提供理论基础,同时为新杀菌剂的研发提供新思路。     

昆虫组蛋白修饰的研究进展

组蛋白修饰作为表观遗传修饰的一种主要形式,对基因表达和表型调控具有重要作用。组蛋白修饰的N端尾区可通过乙酰化、甲基化、磷酸化等修饰来改变染色质的状态以及调控基因的表达。与脊椎动物相比,昆虫种类繁多,且有变态发育、表型复杂等特征,可以成为探索动物社会行为、发育调控和毒理作用等表观遗传基础的模型。本文总结了昆虫组蛋白修饰的主要类型(乙酰化和甲基化修饰)及修饰酶的研究进展,对染色质免疫共沉淀测序技术(chromatin immunoprecipitation followed by sequencing,ChIP-seq)、染色质转座酶可及性测序技术(assay for transposase-accessible chromatin with high-throughput sequencing,ATAC-seq)、转录组测序技术(RNA-seq)、组蛋白修饰酶功能验证以及Western blot、免疫细胞化学(immunocytochemistry,ICC)、免疫组织化学(immunohistochemistry,IHC)、酶联免疫吸附测定(enzyme linked immunosor...     

我国农田杂草治理技术的发展

我国稻、麦、玉米、大豆及棉花等主要农作物农田杂草有580种,属于危害重而又难于防除的有17种,受杂草危害较重的农田有3 580万hm²,年平均受草害减产12.3％～16.5％(加权平均)。自1980年以来,全国化学除草面积以年增200万hm²的速度扩大,农田化学除草面积从20世纪70年代早期不到100万hm²扩大到近年来6 000万hm²。本文概述了我国农田主要作物杂草的发生危害、化学除草剂应用现状及其综合防治进展,并提出今后杂草防除的设想与建议。     

昆虫紫外视觉研究进展

昆虫在长期进化过程中形成的颜色视觉能力，是其直接获取外界信息的最主要途径之一。其中，紫外视觉在昆虫颜色视觉系统中普遍存在，通过紫外光感受器可以感知并辨别周围环境中光源或物体反射的特定波长紫外光，提高颜色辨别能力。自19世纪80年代证实昆虫具有紫外敏感性以来，陆续发现蜜蜂、蝴蝶、果蝇和蛾类等许多昆虫具有良好的紫外视觉能力，利用颜色、对比度和偏振光等紫外光特性调控昆虫的定向导航、觅食、种内通讯和昼夜节律等行为活动，对于其生存、繁殖和生态适应具有重要意义。此外，紫外敏感视蛋白是昆虫紫外视觉形成过程中的一类重要分子，不仅可以独立介导趋光性等特定波长行为，还可纳入整个颜色视觉系统，与其他视蛋白协同调控昆虫的颜色视觉，从而将紫外光当作一种基本颜色进行辨别。该文系统综述了昆虫紫外视觉的发现、特性、功能和感知机制，鉴于当前国内在相关领域研究的薄弱和局限性，建议今后可在解析植物-害虫-天敌生态系统中的紫外光特性、探究紫外光-B紫外视觉的生态学意义、揭示紫外敏感视蛋白基因的分子进化机制以及阐明昆虫避免或修复紫外光所致视网膜损伤相关适应机制方面进行深入研究。     

Variability in initial spray deposit in apple trees in space and time

Variation in pesticide residues in space and time has not been investigated systematically in spite of the large variability found at single scales (e.g. between trees or orchards). Information on variability at different scales will support the development of sampling methods and more reliable prediction of residues. Experiments were conducted to quantify the spatiotemporal variability in initial spray deposit on apple leaves and fruit using a substitute tracer (zinc EDTA chelate). Five hierarchical spatial scales were defined as (1) between orchards, (2) between plots within an orchard, (3) between trees within a plot, (4) between zones within a tree and (5) between leaves/fruit within a zone. Similarly, two temporal scales were defined as (1) between applications within a single year and (2) between years. The initial zinc concentration was approximately log-normally distributed; the variability in initial deposit concentration was greater on leaves than on fruit. The average initial zinc deposition was significantly greater in the top and outside zones than in the middle zones within individual trees. The most important scale for driving residue variability is the within-zone unit-to-unit variation, contributing to 71 and 49% of the observed variability for leaf and fruit samples respectively. Variability at other scales was related to the variation in the tree architecture. The results indicated that stratified sampling on the basis of within-tree zone structures, with the effort focused on within-zone sampling, should be used for the determination of pesticide residues.     

西安地区丰水年农田深层土壤含水量研究

通过对西安南郊吴家坟、长安区郭杜镇大学城和双竹村附近丰水年(年降雨量883 mm)麦地、玉米地土壤含水量的测定,以及与正常年份该地土壤含水量的对比,研究丰水年该地区农田0~6 m土壤含水量的变化。结果显示,正常年份西安地区麦地0~2 m土层含水量高于12%,2~6 m土层含水量高于13%;丰水年西安地区麦地0~2 m的土壤含水量在18%以上,2~6 m土层含水量达24%以上,远远高于正常年份这一层位的土壤含水量。分析得出,西安地区农田深层土壤含水量高的主要原因是重力水带分布范围大于或等于农作物的强烈耗水层范围。     

近54 a来甘肃省河东地区气候时空变化特征

用甘肃省河东及其周围地区近54 a来26个观测站气温、降水量资料,分析气温和降水的时空变化特征及其突变情况。结果表明：近54 a来甘肃河东地区气温呈明显的上升趋势,年平均气温倾向率为0.26 ℃/10 a,特别是20世纪90年代以来气温明显升高;空间分布上,年平均气温变化总体上呈增加趋势,以陇东高原中北部和陇中高原北部地区升温幅度最为明显;季节上气温均呈升高趋势,其中以冬季气温升高最为显著。近54 a来全区年平均降水量呈减少趋势,平均每10 a减少12.01 mm,其东南部地区减少的幅度高于西北部。此外,甘肃河东地区气温在1997年发生了由冷到暖的突变,而降水量突变不明显。进而讨论了气温升高和降水减少对该地区农业生产的影响。     

中国植物弹状病毒研究进展

弹状病毒含有单链负义RNA基因组,寄主范围比较广泛,能侵染无脊椎动物、脊椎动物以及植物等寄主,对人类的健康、农作物产量和自然生态系统造成严重威胁。植物弹状病毒主要根据复制场所分为细胞质弹状病毒属Cytorhabdovirus和细胞核弹状病毒属Nucleorhabdovirus,其基因组由单股负链RNA组成。此外,Dichorhavirus和Varicosavirus病毒属是两个新鉴定的植物弹状病毒属,其特征是具有二分体基因组。本文着重介绍危害我国农作物的几类植物弹状病毒,对它们的病理学、病害流行、基因组信息以及传播媒介进行概述,为中国植物弹状病毒病害的研究提供理论依据。     

近年我国农田杂草防控中的突出问题与治理对策

我国田园杂草有1 400多种,严重危害的130余种,恶性杂草37种。我国杂草发生面积约9 246.7万hm2次,防治面积1.04亿hm2次,挽回粮食损失2 699万t,每年主粮作物仍有近300万t产量损失。杂草防控中的突出问题是:杂草群落演替,难治杂草种群增加;除草剂单一使用,杂草抗药性发展迅速;除草剂对作物药害频发,影响种植结构调整;新除草剂创制能力不足,难以满足不同作物田除草需求;农村劳动力短缺,杂草防控更依赖于化学防治。解决上述问题,应实施以下对策:加强杂草发生危害的监测预警,科学轮换使用除草剂,推广除草剂减量与替代技术,加快新除草剂研制及推广应用,加速耐除草剂作物商业化进程,推进统防统治及农民培训。     

Phytopathological situation in potato in Belarus1

The control of potato diseases in Ukraine is reviewed. Control of potato blight (Phytophthora infestans) has only been adequate for limited periods in the past (when resistant cultivars were available and when the systemic fungicides were introduced). Currently, the disease is in a new epiphytotic phase and control has failed again, probably connected with the introduction of new mating types and the existence of greater variability. Blight now occurs earlier in the season and in most years (instead of only years when climatic conditions are favourable). Control has now to be essentially preventive, rather than based on forecasting. The importance and control of other potato diseases is also considered (Alternaria spp., Rhizoctonia solani, Geotrichum candidum, Phytophthora erythroseptica, Sclerotinia sclerotiorum, Streptomyces scabies).     

2009年黑龙江和吉林省马铃薯晚疫病菌表型结构研究

为明确2009年黑龙江和吉林两省马铃薯主产区晚疫病菌的表型特征,本研究对所采晚疫病菌的交配型、甲霜灵抗性及生理小种分别进行了测定.结果表明:被测黑龙江(46株)和吉林(48株)两省的菌株均为 A1 交配型.黑龙江省被测菌株甲霜灵高抗、敏感菌株各占50％;吉林省被测菌株中高抗、敏感菌株分别占70.8％、29.2％;两省均未发现中抗菌株.其中采自吉林敦化菌株均为高抗菌株,采自吉林榆树的菌株均为敏感菌株,甲霜灵抗/感比例地区间差异明显.在被测33个黑龙江菌株中发现24个生理小种,而吉林49个菌株中发现19个生理小种,其中9个生理小种两省共有.另外,两省被测菌株中还发现了18株“超级毒力小种”.因此,建议加强种薯监测,防止 A2交配型传入;在生产上使用甲霜灵类药剂防治马铃薯晚疫病应该以菌株的抗性监测数据为依据;进一步加强生理小种监测,合理布局抗病品种.     

Major Changes in Fusarium spp. in Wheat in the Netherlands

The re-emergence of fusarium head blight throughout the world and especially in Western Europe prompted a survey of the situation in the Netherlands. To allow for a high throughput screening of large numbers of samples, a diagnostic PCR method was developed to detect the most common species of Fusarium occurring on wheat. Seven primer pairs were tested for their ability to identify isolates of Fusarium avenaceum, F. culmorum, F. graminearum, F. poae, F. proliferatum and Microdochium nivale var. majus and M. nivale var. nivale. Each primer pair only generated a PCR product with the corresponding Fusarium species and all PCR fragments had different molecular sizes. This allowed the generation of these amplicons using a mixture of all seven primer pairs. The robustness of this multiplex PCR encouraged us to screen a large series of isolates collected in 2000 and 2001. In both years 40 fields were sampled leading to a collection of 209 isolates from 2000 and 145 isolates from 2001. The results of the multiplex PCR demonstrated that F. graminearum was the most abundant species in the Fusarium complex on wheat in both years. This is in sharp contrast to reports from the 1980s and early 1990s, which found F. culmorum as the predominant species. Primers derived from the tri7 and tri13 genes, which are implicated in the acetylation and oxygenation of the C-4 atom of the backbone of the trichothecene molecule, were used to discriminate between deoxynivalenol and nivalenol (NIV) producers. The populations of F. culmorum and F. graminearum both showed a slight increase in NIV-producers in 2001.     

Insecticide resistance in vector mosquitoes in China

Because of their special behaviour, physiology and close relationship with humans, mosquitoes act as one of the most important vectors of human diseases, such as filariasis, Japanese encephalitis, dengue and malaria. The major vector mosquitoes are members of the Culex, Aedes and Anopheles genera. Insecticides play important roles in agricultural production and public health, especially in a country with a huge human population, like China. Large quantities of four classes of insecticides, organochlorines, organophosphates, carbamates and pyrethroids, are applied annually to fields or indoors in China, directly or indirectly bringing heavy selection pressure on vector populations. The seven major species of vector mosquito in China are the Culex pipiens L. complex, C. tritaeniorhynchus Giles, Anopheles sinensis Wied., A. minimus Theobald, A. anthropophagus Xu & Feng, Aedes albopictus (Skuse) and Ae. aegypti L., and all have evolved resistance to all the above types of insecticide except the carbamates. The degree of resistance varies among mosquito species, insecticide classes and regions. This review summarizes the resistance status of these important vector mosquitoes, according to data reported since the 1990s, in order to improve resistance management and epidemic disease control, and to communicate this information from China to the wider community.     

Differences in weed vegetation in response to cultivating methods and water conditions in rainfed paddy fields in north-east Thailand

Rainfed paddy cultivation predominates in north-east Thailand. Direct dry seeding is replacing transplanting rice cultivation to increase the frequency of successful plantings and to save labor. The present study clarified differences in weed vegetation of direct dry-seeded and transplanted paddy fields under rich, medium and poor water conditions from agricultural and ecological viewpoints. A large number of paddy fields from a wide range of climatic, topographic, soil and hydrological conditions were used. A survey was conducted every three weeks, and data regarding paddy cultivation, weeds and water conditions were collected. Expansion of direct dry seeding may increase species with in-between water adaptability to hygrophytes and mesophytes, and decrease hydrophytes and mesophytes. This characteristic was remarkable under poor water conditions. Rice cover was similar in either planting method under rich water conditions. However, cover of direct-seeded rice was significantly smaller than that of transplanted rice under medium and poor water conditions. Species diversity was higher in direct dry-seeded paddy fields than in transplanted paddy fields under medium and poor water conditions. Impact of direct dry seeding in the fields with rich water conditions on the vegetation is small in the context of maintaining rice production by transplanting and species diversity. However, direct dry seeding is mainly adopted in fields with poor water conditions. Impact of the changes in cultivating methods is large on rice productivity, species composition and species diversity under such conditions. Therefore, how to best use resource-poor fields may be the key to maintaining rice production and regional-level species diversity.     

Fluctuations in Sunn-pest Populations in South-eastern Anatolia

Sunn pest ( Eurygaster integriceps ) is the most serious and widespread pest of cereals in south-eastern Turkey. It is very important to estimate its spring populations, and the area possibly needing to be treated, a few months before they migrate to the fields. The field populations of the pest and the severity of epidemics can be forecast from the population density of overwintering adults per plant in the overwintering localities. To do this, surveys have been made since 1955 on 25 selected mountain sites. Parallel to these investigations, the population of overwintered adults per m2 in cereal fields has been determined in order to fix the area to be treated, since 1956. A relation can be seen between the population density of overwintered adults per plant and the next season's populations in the infested areas.     

Insecticide resistance in Helicoverpa armigera in South India

Cypermethrin, quinalphos, endosulfan and methomyl were bioassayed against strains of Helicoverpa armigera collected from field crops in Andhra Pradesh and Tamil Nadu, South India, during the 1989-90 and 1990-91 cropping seasons. In 1989, high levels of resistance to cypermethrin were recorded in strains from cotton in the cotton-growing regions of Guntur, Andhra Pradesh and Coimbatore, Tamil Nadu, and from pigeon pea near Hyderabad. There was no evidence for resistance to quinalphos or methomyl at that time. In 1990-91 sampling was more extensive and although tolerance to cypermethrin was lower than the previous season, the survey indicated that pyrethroid-resistant populations were present throughout much of Andhra Pradesh. Tolerance to quinalphos had increased slightly in 1990-91, while resistance to methomyl had increased substantially, particularly in the cotton-growing area of Guntur. Endosulfan tolerance had increased slightly compared to strains tested in 1986-88 in an earlier study. The geographic and temporal variations in severity of pyrethroid resistance in H. armigera in Andhra Pradesh are believed to arise because of dynamic interactions between local selection pressure and immigration of resistant and susceptible moths at certain times of the year.     

Rapid in vitro microindexing of viroids in citrus

A new laboratory technique combining shoot-tip grafting in vitro and biological indexing on indicator plants was explored for the detection of citrus exocortis and related viroids. Τhree in vitro laboratory methods were used and compared with the classical biological method. With the classical in vivo method, diagnosis is based on the expression of symptoms on indicators 11–14 weeks after inoculation. In contrast, with the first in vitro method, microindexing in vitro of citron seedlings by graft inoculation, diagnosis was possible 12 days after inoculation; with the second method, microindexing in vitro of citron cuttings by graft inoculation, 20 days after inoculation; and with the third method, microindexing in vitro of citron cuttings by injection inoculation, 40 days after inoculation. Inoculated Etrog citron plantlets grown in vitro and tested by RT-PCR showed the same viroid content as the source plants. Of the three in vitro viroid indexing methods, microindexing on cuttings by grafting was easier and more reliable than microindexing either on seedlings or on cuttings by injection.     

湖南省棉田杂草调查

对湖南省棉花主产区棉田杂草的种类、发生规律及化学除草情况进行了调查。结果发现, 湖南省棉田常见杂草包括12科, 28种, 其中禾本科杂草占25.00%, 阔叶杂草占67.86%, 莎草科占7.14%; 一年生杂草占78.57%, 多年生杂草占21.43%。相对多度10%以上的杂草有7种, 其中马唐、牛筋草、千金子、铁苋菜、小飞蓬5种杂草为湖南省棉田杂草的优势种, 相对多度分别为37.79%、33.64%、29.15%、26.23%、23.83%。杂草发生呈现3个高峰期, 分别为5月下旬, 6月中下旬, 7月下旬至8月初, 杂草防除主要采用苗前土壤处理和苗后茎叶处理相结合的化学防除方法。