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在96孔微板中包被抗原,加入被检血清,经温育后,抗体特异性地与抗原相结合,形成抗原一抗体结合物。洗涤去除未结合的物质,与加入的酶结合物(HRPO)相结合。再洗去未结合的酶结合物,加入底物后显色.加入终止液终止反应。用装有630nm(或620nm、650nm)滤光片的酶标仪进行检测,通过计算S/P值,判定血清中副结核抗体阴、阳性。此方法具有快速、灵敏、准确的特点。 相似文献
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R.W. Sweeney M.T. Collins A.P. Koets S.M. McGuirk A.J. Roussel 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2012,26(6):1239-1250
Paratuberculosis (Johne's disease) is a widespread and costly disease. This consensus statement will summarize recommendations regarding diagnosis, control, and treatment of Johne's disease in cattle and other species. Each section of recommendations is followed by a statement that subjectively characterizes the strength of the supporting evidence. The role played by Mycobacterium avium subsp. paratuberculosis (MAP) in the pathogenesis has been a matter of controversy for many years. This statement concludes with an assessment of the evidence in favor of MAP as a potential zoonotic pathogen. 相似文献
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羊副结核病是由副结核分枝杆菌引起的一种以顽固性腹泻、肠粘膜显著增厚为主要临床特征的慢性传染病,多呈现隐形感染,该病潜伏期长,传播迅速,呈全球性分布,对畜牧业的危害巨大。本文立足羊副结核病研究进展的实际情况,梳理总结了目前学术界对羊副结核病病原学、流行病学、致病机制、临床诊断和免疫综合防控等方面的研究讨论,为羊副结核病的诊断与防控提供了理论基础。在此基础上提出防控羊副结核病政府需制定有效政策,养殖场需加强饲养管理,形成科学有效的防控系统,为我国畜牧业的可持续健康发展提供了理论支撑。 相似文献
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Epidemiology and Economics of Paratuberculosis 总被引:2,自引:0,他引:2
Jason E. Lombard 《Veterinary Clinics of North America: Food Animal Practice》2011,27(3):525-535