Gas exchange of Rhodiola sachalinensis transplanted from different habitats in Changbai Mountain

Introduction1RhodiolasachalinensiSA.Bor,aperennial,medicalherb,mainlydistributesinJapan,Korea,ChinaandRussia.InChina,itmainlydistributesinChangbaiMOuntain,JinnProvince,andZhangguangcaiMountain,HeilongjiangProvince.ThistypicalalpineplantgenerallydistributedinhighMountain,ataltitude1700-25o0m(Wuetsl1987,Qin19941Luetal1995).Rhodiolsplant(RhodiOlaroseschieflyinRussia)hasbeenextensivelystudiedbyscholarsfromRussia,Ukraine,JapanandChinasincethe1950's(Ming1988iKir'yanovetsl1991,OhbaandMid…     

Diurnal change of gas exchange ofRhodiola sachalinensis transplanted from different habitats in Changbai Mountain

Rhodiola sachalinensis growing in the different habitats, such as Xiaotianchi plot (altitude 1800 m), Tree line plot (altitude 2000 m) and Tianwenfeng plot (altitude 2325 m) of Changbai Mountain (northern slop) were transplanted to Harbin Experimental Farm for determining its gas exchange. The study results indicated that the diurnal changes of gas exchange ofRh. sachalinensis transplanted from different habitats were still different though the morphological appearance of the newly sprouted above-ground part had become more similar. For net photosynthesis rate (Pn), stomatal conductance (Gs) and apparent quantum yield (AQY), the diumal change curves ofRh. sachalinensis transplanted from the three plots were similar, but the data value were different. For the dark respiration rate (Rd), transpiration rate (Tr) and water use efficiency (WUE), diurnal change curves of the three plots were obviously different. This study is supported by Natural Science Foundation of Heilongjiang Province. Yan Xiufeng, male, born in March 1965, associate professor, Open Research Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, P. R. China. Responsible editor: Zhu Hong     

Gas exchange ofRhodiola sachalinensis transplanted from different habitats in Changbai Mountain

Rhodiola sachalinensis growing in the different habitats, Xiaotianchi plot (altitude 1800 m), Tree line plot (altitude 2000 m) and Tianwenfeng plot (altitude 2325 m), of Changbai Moutain (northern slop) were transplanted to Harbin Experimental Forest Farm for measuring its characteristic of gas exchange. The study results indicated that the growth state and gas exchange characteristics ofRh. sachalinensis growing in different habitats varied markedly. The plants transplanted from Tianwenfeng plot had the highest values in net photosynthesis rate (Pn) and transpiration rate (Tr), those transplanted from Tree line plot shows the second, and those transplanted rom Xiaotianchi plot had the lowest values. The variance existed in transplanted plants was the same as shown in the field. From the result it can be extrapolated that the difference of Pn and Tr existed inRh. sachalinensis transplanted from different habitats is depend not only on the environmental factors, but on the variance of physiological characteristic of plant itself. This study is supported by Natural science Foundation of Heilongjiang Province. Responsible editor: Chai Ruihai     

高山红景天愈伤组织的超低温保存

高山红景天叶片在附加6-BA 2 mg·L-1和NAA 0.5 mg·L-1的改良MS培养基中诱导出愈伤组织,将愈伤组织放入10%DMSO 0.5 mol·L-1山梨醇的冰冻保护剂中,4℃冰箱中放置8 h,然后以1℃·min-1的降温速度降至-40℃,停留2 h后投入液氮保存,在40℃水浴中化冻.冻存后的愈伤组织用液体培养基MS 6-BA 2 mg·L-1 NAA 0.5 mg·L-1反复冲洗后,转入固体培养基中进行暗培养,2周后转入光照(2 000 1x)培养.5周后观察到高山红景天愈伤组织呈鲜绿色,长势较为旺盛,愈伤组织存活率达到75.64%.冻存后的愈伤组织可诱导成苗.     

高山红景天育苗技术

采用低温(-18℃)、流水冲洗、GA3等不同方法处理种子,低温处理发芽效果最佳;秋播比春播好,按1年生苗根重分3级,确定苗圃定苗量为500株/m2,用多菌灵、菌核净、多菌灵 退菌特和402抗菌剂处理立枯病防治效果达到了80%以上。     

不同植物生长调节剂对高山红景天种子萌发的影响

研究了赤霉素和吲哚丁酸处理对高山红景天种子萌发的影响。结果表明,赤霉素和吲哚丁酸处理均能显著地提高高山红景天种子的发芽率和发芽势,以赤霉素处理的促进效果最为明显,赤霉素以400mg/L处理的发芽率和发芽势最大,分别为62.41%和48.24%;IBA以100mg/L处理的最大,分别为55.48%和36.84%。赤霉素和IBA不同浓度处理间高山红景天种子的发芽率和发芽势存在极显著性地差异。     

超临界CO_2萃取-微波辅助提取红景天苷的研究

采用超临界CO2萃取-微波辅助法,研究了从红景天中提取红景天苷的工艺条件。结果表明:适宜的超临界CO2萃取工艺条件为:5.000 g红景天粉末,萃取压力40 MPa,萃取温度55℃,萃取时间5 h,夹带剂为无水乙醇;萃取后红景天1.000 g,加入30 mL超纯水,浸泡2.0 h,微波功率为180 W,微波处理2 min,红景天苷提取率高于95%。     

Relationships of climate change and tree ring of Betula ermanii tree line forest in Changbai Mountain

Based on the tree-ring growth characteristics of Erman＇s birch （Betula ermanii charm.） and the relationships between it and climatic ）＇actors at elevation of 1950m, the sensitivity of tree lines in Changbai Mountain to climatic factors was assessed. The results indicated tree line forest in Changbai Mountain had an obvious sensitivity to climate factors. However, difference from other study sits is that the main climatic control factor on tree-ring growth was not current growth season temperatures, as might be expected, but previous winter and current March temperature. Although the precipitation in the region was quite abundant, the tree-ring growth was still significantly correlated with the precipitation during previous winter and current spring. Additionally, climatic factors which influenced the Erman＇s birch growth were not the yearly variables, but seasonal and monthly variables. Therefore, the reported increase in yearly mean temperature and total yearly precipitation since 1980s was not responded by sustained increase in ring widths in recent decades.     

Turbulent exchange of CO2 over a broadleaf-Korean pine forest in Changbai Mountain, northeast China

Turbulent exchange of CO₂ was measured continuously via the open-path eddy covariance technique over a broadleaf-Korean pine forest in Changbai Mountain, northeast China. The results show that with near-neutral atmospheric stratification, CO₂ and vertical wind components measured over the forest canopy in the inertial sub-range followed the expected −2/3 power law. The dominant vertical eddy scale was about 100 m. The frequency ranges of eddy contributions to CO₂ fluxes were mostly within 0.01–2.0 Hz. Large eddies with low frequency over the canopy contributed more to CO₂ fluxes than small eddies. The open-path eddy covariance system could satisfy the estimation of turbulent fluxes over the canopy, but the CO₂ fluxes between forest and atmosphere were generally underestimated at night because of the increment non-turbulent processes, suggesting that the CO₂ fluxes estimated under weak turbulence need to be revised correspondingly. __________ Translated from Chinese Journal of Applied Ecology, 2007, 18(5): 951–956 [译自: 应用生态学报]     

长白山阔叶红松林冠层透射率的定点观测研究

根据长白山阔叶红松林林上和林地太阳辐射的定点观测结果 ,计算了 5月 2 2日— 10月 14日的林冠透射率 ,发现林冠定点透射率与天空状况有关 ,阴天时透射率的日变程曲线比较平稳 ,少云天波动增加 ,晴天条件下波动最为剧烈 ,这种差别是由林窗和太阳辐射中直接辐射的比率不同而产生的。然而通过 2d定点测定与游走测定的比较发现 ,两种方法测定的透射率日平均值差异不大 ,定点测定的日平均值能较好地代表林冠的透射率特征。定点透射率的日变程曲线还随着生长季进程而变化 ,不仅透射率大小发生变化 ,透射率峰值的出现时间也发生更迭。在观测期间内 ,定点透射率日平均值的季节变程曲线呈U形 ,5月底和 10月上旬较高 ,7— 8月较低且平稳 ,随日期 (季节 )的动态变化符合四次多项式函数 ,反映了冠层叶面积的季节变化特征。     

Determination of berberine in Phellodendron amurense from different sites of Changbai Mountain

Phellodendron amurense has been used for many years as a medical plant in traditional Chinese medicine and has shown great prospect in recent clinical trials for future applications. Berberine is an essential active compound contained in P. amurense. Our objective in this study was to quantify the content of berberine in P.amurense from sites at different elevations on Changbai Mountain. We collected samples of P. amurense from five different elevations on Changbai Mountain. Berberine in samples was extracted by ultrahigh pressure extraction(UPE). And the quantity was measured by high performance liquid chromatography(HPLC). First, the optimal HPLC conditions for berberine were identified with satisfactory precision(relative standard deviation, R SD5.6 %), good accuracy(relative error, R E 3.6 %) and good linear relation(R2= 0.9998) in the range of 6.576–328.8 mg L-1. Second, the combination of UPE and HPLC methods in quantitative analysis of berberine showed high repeatability(R SD= 3.28 %), reproducibility(R SD= 4.72 %),stability(R SD 1.27 %) and good recovery(99.54 %) for real plant materials. Samples from Heilongjiang Province at the lowest elevation contained the highest amount of berberine.Similarly, the lowest amount of berberine was recorded in samples from Changbai Forest Bureau of Jilin Province collected at the highest elevation in this paper. The proposed UPE–HPLC method is simple, reliable and low-cost for quantitative analysis of berberine. Content of berberine in P.amurense varied significantly by site on Changbai Mountain.     

Forest vegetation responses to climate and environmental change: A case study from Changbai Mountain, NE China

The distribution of plant species has always been altered by changing climatic conditions. Nonetheless, the potential for species’ range shift responses has recently become severely limited, as exceptionally fast temperature changes coincide with a high degree of anthropogenic habitat fragmentation. This study provides rare insights into the effects current temperature increases have on pristine temperate forest ecosystems, using the forests of Changbai Mountain, NE China, as a case study. On the northern slopes of the mountain at elevations between 750 and 2100 m, the composition of trees, shrubs and herbaceous species was recorded on 60 plots in 1963 and 2006/07. Multiple linear regression (MLR) and canonical correspondence analysis (CCA) were used to establish the response of plant diversity and plant distribution patterns to environmental conditions. Climatic factors proved important in explaining the spatio-temporal trends within the vegetation. The composition of dominant trees remained mostly unchanged over the last 43 years, reflecting a very slow response of the forest canopy to environmental change. The composition of young trees, shrubs and herb species showed varied changes in the different forest types. A homogeneous species composition in the cohort of regenerating trees indicates an increased future uniformity in the mixed broadleaved and coniferous forest. The understory vegetation of high elevation birch forests was invaded by floristic elements of the lower-elevation coniferous forests. Both these trends pose potential threats to forests plant diversity. Future research investigating climate change responses in forest canopy composition needs to be based on even longer timescales, while investigations in the near future need to pay particular attention to the changes in the distribution of rare and threatened herbaceous species.     

Effects of light quality on gas exchange and dry matter partitioning in Eucalyptus grandis W. Hill ex Maiden

Stockplants of Eucalyptus grandis were pruned to a height of 7–10 cm and after 3 weeks were placed in growth cabinets set at a photon flux density (PFD) of 200 μmol m⁻² s⁻¹ and red to far-red ratios of 0.4, 0.7, 1.3, 3.5 or 6.5. Experiments tested the effects of light quality on growth and gas exchange of stockplants. Light quality did not affect the total shoot dry weight (DW), root DW or shoot to root ratio of stockplants or their total leaf area. However, there were significant effects of light quality on: (i) plant height, which was greatest at red:far-red (R:FR) ratios of 0.4 and 0.7; (ii) partitioning of DW between leaves and stems, with greater stem DW and less leaf DW at low R:FR ratios (0.4 and 0.7); (iii) partitioning of DW and leaf area between the most dominant shoot and all other (non-dominant) shoots; (iv) specific leaf area, which was greatest at low R:FR ratios. In the above characters, the dominance ratio (ratio of most dominant shoot to sum of all other shoots) was greatest at low R:FR ratios and least at ratios of 3.5 and 6.5. Photosynthetic rate per unit leaf area and leaf chlorophyll concentration significantly increased with increasing R:FR ratio. However, photosynthesis per unit chlorophyll concentration was significantly greater at low R:FR ratios. Generally, light quality had no significant effect on photosynthetic rate per leaf or per unit dry weight, but rates of transpiration, stomatal conductance and water use efficiency increased with an increase in R:FR ratio. These data indicate that compensatory changes in plant morphology and gas exchange caused equality in total dry weight per plant between treatments. The above effects of light quality on dry matter partitioning and gas exchange had important effects on the size, number, morphology and physiology of subsequently collected cuttings for vegetative propagation.     

长白山典型林区森林资源变化及目前质量分析

利用研究区1985年和1999年两期遥感数据,将两期图像的矢量化图像中变动幅度较大的主要类别进行叠加,通过图像单元统计及图像叠加分析,得知各类地物在十五年间的变化情况及相应类别变化的量化归属。研究表明,十五年间采伐迹地、人工幼林变化剧烈,人类活动是造成研究区地物剧烈变化的最主要原因。选择研究区中二个干扰与经营状况不同的林场进行重点剖析,结果表明经营强度及方式的不同,对森林资源质量有较大的影响。东升林场采伐利用强度相对较小,目前仍保存有面积约占该林场1/3左右的地带性植被,过熟林面积比例较高,成熟针叶林单位面积蓄积量高达536.27m3hm-2,与长白山自然保护区的阔叶红松林单位面积蓄积量大体相当;西林河林场因采伐强度较大,目前林分多为原始林被破坏后形成的次生林,成过熟林比例相对较低,单位面积蓄积量也较小。二个林场森林状况以及营林措施方面具有一定代表性,其森林演替具有天然、人为干预两个方面的性质,作为重点剖析对象对研究区未来森林资源经营具有一定的指导意义。本文还就研究中发现的问题进行了剖析,并对当地的营林策略提出了可行性建议。图6表4参13。     

Quality and change analysis of forest resource in typical Changbai Mountain forest region

The utilization and changes of forest resources were studied in the Lishuihe Forest Bureau. Based on remote sensing images in 1985 and 1999, changes of major forest resources were analyzed by statistical and overlap method and classified quantitatively. The results showed that in recent 15 years, logging spots and man-made young forest changed violently, which was due to human activities. Different forest management manners and harvesting intensity played an important role in forest resources change. Dongsheng and Xilinhe tree farms were typical cases of different forest status and management for the Bureau, where forest succession was intervened by either human or natural disturbance. Dongsheng Tree Farm underwent a lighth arvest intensity and maintained a unit stock volume of 536.27 m^3. hm^-2, as much as that of broadleaf/Korean pine forest of Changbai Mountain Natural Reserve; Xilinhe Tree Farm underwent an intense harvest and was composed of secondary forests,where mature forests just had a small percentage and the unit stock volume was low. The study was useful to guide future forest management. What‘s more, problems found in the research were also analyzed and reasonable advice was given to the local forest management.     

Influence of canopy position, needle age and season on the foliar gas exchange of Pinus canariensis

We investigated the seasonal variation in the gas exchange of current and 1-year-old needles in the upper sun and lower shade crown of adult Pinus canariensis trees. In general, current year needles displayed lower gas exchange rates than the 1-year-old needles. In both needle age classes, gas exchange was significantly lower in the shade than in the sun crown. However irrespective of crown position and needle age, maximum daily net photosynthesis, transpiration, and stomatal conductance for water vapour were generally higher during the wet and cold winter as compared to the dry and hot summer. These higher gas exchange values obtained during the cold and wet season can mainly be explained by higher soil-water availability and lower evaporative demand as compared to the warm and dry seaon. In addition, we also observed a displacement in the temperature optimum of net photosynthesis towards lower temperatures during the cold and wet season as compared to the warm and dry season. The observed gas exchange characteristics indicate a conservative water saving strategy and thus allowing P. canariensis needles to maintain a positive carbon gain even at periods of high evaporative demand and low soil-water availability.     

Nitrification and denitrification as sources of gaseous nitrogen emission from different forest soils in Changbai Mountain

The contributions of nitrification and denitrification to N₂O and N₂ emissions from four forest soils on northern slop of Changbai Mountain were measured with acetylene inhibition methods. In incubation experiments, 0.06% and 3% C₂H₂ were used to inhibit nitrification and denitrification in these soils, respectively. Both nitrification and denitification existed in these soils except tundra soil, where only denitrification was found. The annually averaged rates of nitrification and denitrification in mountain dark brown forest soil were much higher than that in other three soils. In mountain brown coniferous soil, contributions of different processes to gaseous nitrogen emissions were Denitrification N₂O>nitrification N₂O>Denitrification N₂. The same sequence exists in mountain soddy soil as that in the mountain brown coniferous soil. The sequence in mountain tundra soil was Denitrification N₂O>Denitrification N₂. Foundation item: This paper was supported by the National Natural Science Foundation of China (No.49701016) and the “Hundred Scientists” Project of Chinese Academy of Sciences. Biography: XU Hui (1967-), male, Ph. Doctor, associate research fellow in Laboratory of Ecological Process of Trace Substance in Terrestrial Ecosystem, Institute of Applied Ecology, Chinese Academy of sciences, Shenyang 110015, P. R. China. Responsible editor: Song Funan     

Response of seedlings of different tree species to elevated C02 in Changbai Mountain

Eco-physiological responses of seedlings of eight species,Pinus koraiensis, Picea koraiensis, Larix olgensis, Populus ussuriensis, Betula platyphylla, Tilia amurensis, Traxinus mandshurica andAcer mono from broadleaved/Korean pine forest, to elevated CO₂ were studied by using open-top chambers under natural sunlight in Changbai Mountain, China in two growing seasons (1998–1999). Two concentrations of CO₂ were designed: elevated CO₂ (700 μmol·mol⁻¹) and ambient CO₂ (400 μmol·mol⁻¹). The study results showed that the height growth of the tree seedlings grown at elevated CO₂ increased by about 10%–40% compared to those grown at ambient CO₂. And the water using efficiency of seedlings also followed the same tendency. However, the responses of seedlings in transpiration and chlorophyll content to elevated CO₂ varied with tree species. The broad-leaf tree species were more sensitive to the elevated CO₂ than conifer tree species. All seedlings showed a photosynthetic acclimation to long-term elevated CO₂. Foundation item: The project was supported by National Key Basic Development of China (G1999043400) and the grant KZCX 406-4, KZCX1 SW-01 of the Chinese Academy of Sciences Biography: WANG Miao (1964-), maie, associate professor in Institute of applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China. Responsible editor: Song Funan     

长白山红松针阔混交林CO2通量的日变化与季节变化

森林是陆地上面积最大的生态系统,它通过光合作用吸收大量的CO2并将其长期地储存于生态系统中,森林生态系统CO2通量的研究备受关注(方精云等,2001; 于贵瑞等,2001; 吴家兵等,2003).