Effect of Ginkgo biloba extract and ginsenosides on the Vascular Activity Substance production in endothelium cells

Though Ginkgo biloba extract(GBE)and ginsennosides(GS)also have notability bioactivity on cardiovascular disease,there is little literature about applying the compound of them to treat cardiovascular disease.After endothelium cells suffering from oxidative injury were treated with serum containing GBE,GS and the compound of them(GG),respectively,production of NO,ET and MDA in them was observed by applying serum pharmacology.Both GBE and GS up-regulate NO level and down-regulate ET-1 and MDA level in endothelium cells suffering from oxidative injury.Anti-oxidation activity increased after GBE and GS were combined.But the NO up-regulation of GG was inferior to that of GBE and GS,which decreased by 29.9% and 66.7% when compared with that of GBE and GS,respectively.The ET-1 down-regulation of GG was superior to that of GBE, but decreased by 11.6% compared with that of GS.The compatibility of GBE and GS do not show synergic effect on NO and ET-1 regulation in endothelium cells and it is valuable to discuss that the compound of GBE and GS is used to treat cardiovascular diseases.     

Example Verification and Experimental Study of Network Model for Room Temperature Prediction during the Fire in Buildings

The network model for room temperature and its implicit differential equation are discussed in this paper. The example verification and experimental study were carried out for the prediction of the room temperature in the software of predicting the properties of smoke flow. The results show that the results of example computation are similar to those obtained by similar software in Japan. The results of computation by the software tally with the experimental ones and accord with the law of development and change of room temperature during the fire.     

Calculation of Constant Pressure Values for Relief Valves in the Fire Suppression Systems of High-Rise Buildings

The calculation of constant pressure values for relief valves in the fire suppression systems of high-rise buildings usually is based on the working pressure of pipe systems by experience.However,this method only is applied in the condition that the relief valves are installed on the effluent pipes of pumps.In order to confirm the proper constant pressure values of relief valves in fire suppression systems,a formula is deduced using hydraulics theory.The applications of the formula in fire suppression systems are also discussed.The constant pressure values for relief valves are directly related to three factors,namely,the flux and pressure of the fire pump,the located height of the relief valves,and the head loss of flux from the pump exit to the inlets of the relief valves.It is necessary to analyze the working condition of fire suppression systems and to be certain of the permissive pressure range for overpressure.     

Experimental Analysis of Cracking Behavior of ConcreteBeams Reinforced with High Strength Bars

Eight concrete beams reinforced with 500MPa steel bars and four reinforced with 400MPa ultra fine grain steel bars were tested under two point symmetrical concentrated static loading to observe the details of crack pattern development on these beams and investigate their cracking characteristics. It was shown that the cracking behavior of the beams with high strength bars was essentially similar to that of common RC flexural members, whereas the computed crack widths using the formula adopted in the Code for Design of Concrete Structures GB 50010 2002 exceeded that of the experiment under the normal service. Furthermore, the formulas for crack spacing and crack width specified in GB 50010 2002 were evaluated through the experimental results and previous studies of sixty seven concrete beams reinforced with high strength bars. On the basis of the calculation model in GB 50010 2002, revised formulas for crack spacing and crack width were proposed. The values calculated by revised formulas were in good agreement with the test results.     

Fault Line Selection for the Distribution System with the Adjustable Arc Suppression Coil

The problem of the faulty line selection with the arc suppression coil(ASC) has existed for a long time.On the basis of analyzing the relationships between the ASC admittance and the admittances of lines,including the faulty line and non-faulty lines,the calculation of the b parameter is presented,in which the state of the ASC is changed during the single-ground existing for the faulty line selection by adjusting its inductance.The value of b for the faulty line is much greater than that for non-faulty lines in both the direct ground and the ground with the resistance.The proposed method is verified with the simulation and is applied to the projection.It is proven that the proposed method is with the high reliability.     

Expression of the N-terminal 99 Amino Acids of Yeast Ribosomal Protein / L3 in Transgenic Wheat Confers Resistance to Fusarium Head Blight

Fusarium head blight （FHB） is a worldwide disease for wheat or barley. The contamination of important agricultural products with the trichothecene mycotoxins such as deoxynivalenol （DON） or 4,15-diacetoxyscirpenol （DAS） produced by Fusarium species poses a major health concern for both human and animals. Transgenic wheat plants expressing a truncated form of yeast ribosomal protein L3 （L3A） are produced via particle gun bombardment. Homozygous lines of transgenic wheat plants display resistance to DON when this mycotoxin is incorporated into the medium in seed germination tests. These transgenic plants are shown to be resistant to Fusarium graminearum infection in greenhouse tests accompanied with reduction in the levels of DON on kernels. The upregulation of the endogenous wheat ribosomal RPL3 gene expressions and the transgene expression of L3A are responsible for the enhanced resistance which correlates with our previous observations in transgenic tobacco plants expressing yeast L3A. Our results demonstrate that yeast L3A can be used to control wheat FHB effectively.     

Chaos expression of neuron dynamic simulation model with various stimulations

Chaos control based disease control has become a new direction for medical research. Chaos control can improve the study of nervous system diseases. To explain the inherent characteristics of such treatment with neurophysiology, a neuron dynamic simulation model, based on the Chay model, was built with MATLAB/SIMULINK. Various stimulations were inserted to analyze the dynamic change of the neuron action potential and its chaos expression. The experimental results show that different internal and external stimulations to the nervous system have great influence on membrane potential and chaos expression, indicating the importance and feasibility of chaos control in disease control.     

Multi classification Method of GA SVM on Identifying Grade of Expansive Soils

Aiming at the fact that parameters in support vector machine(SVM) model were difficult to be identified, a genetic algorithm SVM(GA SVM) was proposed to avoid the blindness in parameter choosing and improve the estimation ability of SVM, in which the parameters in SVM and kernel function were searched by genetic algorithm. And it was then applied to the classification for the swell and shrink grade of expansive soils. Five indexes including liquid limit, total swell shrink ratio, plasticity index, water contents and free expansive ratio were adopted as discriminated factors. And the four grades of the expansive soils were the outputs correspondingly. Classification function was obtained through training a large set of expansive samples. And it was shown that the classification method of GA SVM was effective and with high accuracy.     

Effect of Nitrite on Anaerobic Metabolism of Phosphate Accumulating Organisms

Two enhanced biological phosphorus removal (EBPR) sludges were used to investigate the effect of nitrite on anaerobic metabolism of phosphate accumulating organisms (PAOs). It is found that tolerance of PAOs to nitrite in two sludges were different. In the sludge with enriched PAOs cultures treating synthetic wastewater, when nitrite is above 10 mg/L, it inhibits VFA-uptake of PAOs, leading to decrease of PHA synthesis and increase of phosphate release. In the sludge with nitrogen and phosphorus removal by shortcut nitrification treating real domestic wastewater, anaerobic metabolism of PAOs is not inhibited even when nitrite is 30 mg/L; however, the presence of nitrite in anaerobic period causes denitrifying bacteria compete for limited carbon sources with PAOs, resulting in decrease of PHA synthesis and phosphate release. For the enriched PAOs cultures, the ratio of P/VFA obviously increases when nitrite is added in the reactor, indicating increased energy requirement for PAOs to take up VFA. The quantification results for PAOs in two sludges using fluorescence in-situ hybridization (FISH) show that the PAOs level is 55% in the enriched PAOs cultures, and 7.6% in the sludge with nitrogen and phosphorus removal by shortcut nitrification.     

Diversity of Sex Types and Seasonal Sexual Plasticity in a Cucumber Germplasm Collection

The sex type of a cucumber plant is determined by the proportion of male, female and hermaphrodite flowers that it bears and is an important factor that affects fruit yield. In this paper, the sex types and seasonal sexual stabilities of 322 accessions of cucumber germplasm were identified. This germplasm collection displayed a great variety of sex types. We used an updated 10-type sex classification system based on the flower types present and the proportion of nodes with pistillate flowers(PNPF). The PNPF ranges of all the accessions were 2.12%–100% in spring and 0–100% in autumn. A total of 81.37% of the accessions had PNPFs of 10%–50% in spring, but most(84.78%) accessions were reduced to 0–20% PNPF in autumn. The range of reduction of PNPF from spring to autumn was 0–67.91%. In other words, most of the germplasm was normal monoecious(31.68%) or subandroecious(62.73%) in spring, but 94.10% of the accessions were subandroecious in autumn. According to the statistical evaluation of the difference in PNPFs between the two seasons, each accession could be classified into one of three groups: seasonally stable, seasonally sensitive and highly seasonally sensitive, accounting for 10.56%, 20.50% and 68.94% of the accessions, respectively. With a few exceptions, the seasonal PNPF differences were positively correlated with the PNPFs in a given season for most accessions. These results provided useful information and materials for sex expression mechanism research and for breeding cucumbers with high and stable yields.     

广东省野百合天然居群的随机扩增多态性DNA(RAPD)分析

本研究选择了中国广东省7个有代表性的山区作为野百合(Lilium brownii F.E.Brown ex Miellez)天然居群的采样点,对采集到的199份样品进行了随机扩增多态性DNA(RAPD)分析,以建立野百合天然居群RAPD分析方法体系以便后续研究,并了解广东省野百合天然居群多态性情况和居群内外个体及野百合...     

Root and Rhizosphere Colonization of Soybean [Glycine max (L.) Merr.] by Plant-Growth-Promoting Rhizobacteria at Low Root Zone Temperatures and under Short-Season Conditions

Co-inoculation of plant-growth-promoting rhizobacteria (PGPR) with B. japonicum has been shown to increase soybean [ Glycine max (L.) Merr.] nodulation, nitrogen fixation, growth and physiological activity at suboptimal root zone temperatures (RZTs). We studied the survival and growth of seven PGPR inoculated on soybean in a sterile rooting medium at three RZTs (25, 17.5 and 15 °C) on a growth bench. The survival of the two most promising strains ( Serratia liquefaciens 2-68 and S. proteamaculans 1-102) was studied under field conditions in methyl bromide fumigated and non-fumigated soils. In general, population densities varied with temperature. PGPR strains generally colonized the rhizosphere and root surface effeciently at higher RZTs; however, S. proteamaculans 1-102 colonized best at a low RZT (15 °C). The population of PGPR applied to the rhizosphere either with or without addition of B. japonicum increased over time in fumigated soil as compared to non-fumigated soil, indicating that the PGPR survive and proliferate better under fumigated conditions. S. liquefaciens 2-68 had higher population densities both on the root and in the rhizosphere, demonstrating, their ability to colonize under short-season conditions. The possible interactions between the two cultivars (Maple Glen and AC Bravor) and the PGPR were generally not significant, despite observations that growth and yield of AC Bravor respond more strongly to PGPR inoculation.     

务川几种野生百合的组织培养

采用正交试验的方法,在MS固体培养基上研究不同浓度蔗糖、6-BA及NAA对淡黄花百合、文山百合及野百合等3种务川野生百合芽增殖的影响,结果表明:淡黄花百合芽增殖的最佳培养基应为:MS +0.5 mg/L 6-BA +0.5 mg/L NAA+4％蔗糖,文山百合的最佳培养基应为:MS +0.5～1.0 mg/L 6-BA +0.5 mg/L NAA +3％蔗糖;野百合芽增殖的最佳培养基应为:MS+ 0.5 mg/L 6-BA+0.1 mg/L NAA+(2％ ～3％)蔗糖.     

朝鲜百合鳞茎诱导及再生体系建立

野生百合种类稀少,个别种类是中国特有,植物离体快速繁殖是保护稀少濒危百合种的有效方法,本研究为了探究一种朝鲜百合快速繁殖的方法。本研究以野生百合朝鲜为试材,以MS培养基为基础培养基,附加不同种类和浓度的植物生长调节物质（6-BA,NAA）诱导丛生芽及再生植株。以朝鲜百合的鳞茎为外植体,确定鳞茎的最佳消毒时间、影响鳞茎芽诱导的最佳激素组合以及不同激素对鳞茎芽增殖的影响。结果表明：最佳灭菌时间为0.1% HgCl消毒10 min;由于内源激素比例不同,鳞茎诱导的最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L;最佳继代培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L;最佳增殖培养基为MS+6-BA 1.0 mg/L+NAA 0.15 mg/L。     

亚洲百合试管苗生根的研究

为了优化亚洲百合生根培养基配方,为亚洲百合转基因植株试管苗的生根移栽奠定一定的基础,以亚洲百合‘普利安娜’（Lilium Asiatic hybrid cv. ‘Pollyanna’）和‘普瑞头’（Lilium Asiatic hybrid cv. ‘Prato’）试管苗为材料,探讨5种不同激素的浓度组合对试管苗生根率、生根数量、最长根长的影响。结果表明：不同植物激素的组合和浓度对亚洲百合的生根有显著影响,且2个品种生根状况有一定的差异。在生根培养基为MS+2,4-D 1.0 mg/L+KT 0.1 mg/L+6-BA 1.0 mg/L+蔗糖30 g/L+琼脂7 g/L时,亚洲百合‘普利安娜’平均生根率达到98.73%,生根数量达到6.53条,最长根长为4 cm;生根培养基为：MS+NAA 1.0 mg/L+TDZ 0.1 mg/L+蔗糖30 g/L+琼脂7 g/L时,亚洲百合‘普瑞头’平均生根率达到92.73%,生根数量达到6.63条,最长根长为3.77 cm。最后得出：亚洲百合‘普利安娜’最适宜的生根培养基为：MS+2,4-D 1.0 mg/L+KT 0.1 mg/L+6-BA 1.0 mg/L+蔗糖30 g/L+琼脂7 g/L;亚洲百合‘普瑞头’的适宜生根培养基为：MS+NAA 1.0 mg/L+TDZ 0.1 mg/L+蔗糖30 g/L+琼脂7 g/L。     

卷丹百合脱毒快繁技术研究

对卷丹百合脱毒苗组培快繁技术体系进行研究。以带LSV病毒的卷丹百合珠芽为材料,通过36℃高温预处理10天,剥取0.2mm大小的茎尖在MS＋2.5mg/L6-BA＋1.5mg/LGA＋0.5mg/LNAA＋0.1g/L活性炭＋0.6%琼脂＋3%白糖的培养基中进行芽诱导培养,经过一次继代培养后,用RT-PCR检测LSV病毒,脱毒率达100%。将脱毒百合苗在MS＋2.0mg/L6-BA＋2.0mg/L2,4-D＋0.1mg/LNAA培养基中诱导愈伤组织及丛生芽,诱导率达100%;在1/2MS＋2.5mg/L6-BA＋0.2mg/LNAA培养基中进行分化、增殖培养,增殖倍数达到4.31;采用MS＋1.2mg/LNAA＋0.5g/L活性炭培养基进行生根培养,产生的根系多而粗壮,移栽时最易成活。     

百合发育过程中鳞茎淀粉含量及SP活性变化

为探讨百合鳞茎内淀粉含量与淀粉磷酸化酶(SP)活性变化的关系,以亚洲百合和兰州百合为试材,对鳞茎发育过程中母鳞茎、新鳞茎内淀粉含量以及SP活性的变化进行测定。结果表明,2种百合母鳞茎淀粉含量展叶期前均呈下降趋势,而后迅速升高,展叶后40天达到最大值,之后2种百合淀粉含量均下降,亚洲百合下降趋势较为明显;新鳞茎淀粉含量总体呈上升趋势,其中亚洲百合新鳞茎淀粉含量在枯萎期达到最大值,而兰州百合在植株半枯期达到最大值。百合发育过程中淀粉的积累与SP的含量呈正相关,发育过程中2种百合淀粉含量的变化趋势与SP变化趋势基本一致,但鳞茎内淀粉含量最大值与SP活性高峰并非同步出现,说明SP与百合鳞茎淀粉合成有关,却非参与合成淀粉的唯一酶。     

麦田土壤中抗铅菌株筛选及其吸附特性的研究

为研究铅污染土壤中微生物对重金属铅的耐受性及去除效果,从铅污染土壤中分离、筛选出2株抗铅菌株(BY-10和FB-6),研究其对铅的吸附特性、最佳生长条件,并对其进行鉴定。结果表明:菌株BY-10和FB-6分别为蜡状芽孢杆菌和团青霉。它们对铅的耐受质量浓度分别高达1 700,2 500 mg/L。菌株的生长条件范围较广,蜡状芽孢杆菌最适温度为35℃,团青霉最适温度为30℃;最适p H值均为7;最适装液量均为80~100 m L(250 m L摇瓶)。在初始铅质量浓度为0~600 mg/L内,随着浓度增加,菌株生长逐渐减缓,在≥400 mg/L的铅培养液中,团青霉与蜡状芽孢杆菌相比对铅的耐受性较强。在铅质量浓度一定时,随着生长时间的延长,菌株对铅的吸附率表现出先增大后减小的趋势,生物吸附量表现出同样趋势。而生长时间一定时,随着铅质量浓度的不断升高,菌株的吸附率逐渐降低,生物吸附量呈上升趋势。在铅质量浓度为150 mg/L时,蜡状芽孢杆菌和团青霉对铅的去除率达到最大,分别为66.25%和74.88%。     

百合雄性生殖器官发育的分子生物学基础

百合的雄性生殖器官花药和花粉较一般植物大,因此是研究植物生殖发育生物学的常用材料之一。近年来,许多研究者在百合花药、花粉和花粉管等雄性生殖器官和不同发育时期通过SDS—PAGE,蛋白质双向电泳,SSH和RT-PCR等手段分离到了部分特异表达的多肽和蛋白及cDNA片段;并且对部分多肽的功能进行了预测和验证。此外,还分离到一些编码百合在受精过程中花粉管与柱头互作的蛋白,并对其功能和可能的作用机制进行了推测。本文不仅介绍了百合雄性生殖器官发育相关的分子生物学机制,而且还对在相应研究过程中存在的一些问题及其在百合育种中的应用进行了探讨。     

一株抗猕猴桃溃疡病的芽孢杆菌分离和筛选研究初报

从猕猴桃根际土壤中分离到的115株芽孢杆菌中,经过皿内拮抗活性试验筛选到12株对猕猴桃细菌性溃疡病有较好抑制效果菌株。进一步单胞分离纯化后,拮抗活性明显增强,尤以B17-4、B56-1和B56-3效果显著。田间药效试验表明：B56-3菌株发酵滤液稀释500倍时,采用刮除病斑涂抹法防治效果达到88%。