Ecology of some fungi associated with an ageing stand of birches (Betula pendula and B. pubescens)

When stydying the growth of different provenances of Betula pendula and B. pubescens it was noted that fruitbody production by many sheathing (ecto-)mycorrhizal fungi followed distinctive patterns in time and place.When saplings of B. pendula and B. pubescens, from seed collected from ranges of locations in western Europe, were grown on a brown earth at latitude 55° 52′ N, autumnal yellowing and defoliation occurred sooner on saplings originating from northern locations (Scandinavia, 60°–66°N) than on those from the south (United Kingdom and Germany, 50°–55°N); the latter group of trees, particularly of B. pubescens, tended to be taller than the former.In the first 6 years after planting Betula spp., more than 90% of the fruitbodies (toadstools, earth-fans of Thelephora terrestris and elf-cups of Peziza badia), usually appearing in the autumn, were of fungi known, or suspected, to form sheathing mycorrhizas with Betula spp. Mean numbers of fruitbodies increased from 0.4 to 28 and 170 per tree in the 2nd, 4th and 6th years respectively after planting. Numbers of fungal species producing fruitbodies progressively increased from one, Hebeloma crustulvniforme, in the 2nd year to 14 (B. pubescens) and 18 (B. pendula) in the 6th year when Hebeloma spp. accounted for 79% of fruitbodies; Laccaria ‘laccata’, 15%; Inocybe lanuginella, 5% and Lactarius pubescens, 1%. Of the 6th year Hebeloma total, H. crustuliniforme, H. fragilipes, H. mesophaeum and H. sacchariolens accounted for 32, 33, 13 and 11% repectively.Mean distances between fruitbodies and the stems of trees widened at c. 0.16 m year^?1 for Hebeloma crustuliniforme; for Laccaria ‘laccata’ they increased by 0.27 to 0.87 m year^?1 between the 4th and 5th years after planting but remained virtually unchanged in the 6th year. Distributions differed within and between concentric 10 cm annuli. In the 6th year appreciably more Hebeloma fruitbodies, except those of H. sacchariolens, were found in intermediate annuli than in annuli nearest and furthest from trees. In contrast those of Laccaria ‘laccata’ and Inocybe lanuginella developed at densities of 1500 m^?2 and 500 m^?2 in most annuli.Fruitbodies of Laccaria ‘laccata’ were significantly clustered in sectors of the differing annuli possibly reflecting associations with radially growing secondarily thickened roots, whereas those of I. lanuginella occurred at random.From 2 to 6 years after planting the mean annual production of toadstools and elf-cups averaged 55 and 0.1 per tree originating from latitude 50° N and 66° N respectively. Earth fans of Thelephora terrestris were likewise less commonly associated with trees of northern origin.B. pubescens, unlike many provenances of B. pendula, seemed to resist invasion by the leaf rust Melampsoridium betulinum. The northern provenances of B. pendula were significantly more seriously infected than southern provenances.     

Fruitbody production by sheathing mycorrhizal fungi: Effects of ‘host’ genotypes and propagating soils

The assemblages of fruitbodies produced by sheathing mycorrhizal fungi associated with clones of Betula spp. were affected by ‘host’ genotype and the origin of propagating soil during the first 6 years after transplanting into a groundwater gley with mull humus.Significantly more fruitbodies were produced when rooted cuttings were propagated in soil A than in soil B, on average 19.5 compared with 4.5 per tree. The larger numbers in soil A were associated with a preponderance of fruitbodies of Laccaria tortilis; Hebeloma spp. being relatively more abundant in soil B.Appreciably more fruitbodies were associated with clone 12.5D of B. pendula than with clone 9.3D of B. pubescens, 19.9 compared with 4.6, the difference being largely attributable to the ‘substitution’ of the prolific L. tortilis by the sparse Inocybe petiginosa.Whereas soil type had a significant effect on numbers of fruitbodies associated with the three more productive clones, it had no effect on numbers of fruitbodies associated with clone 9.3D.     

Biomass production and chemical composition of Moringa oleifera under different planting densities and levels of nitrogen fertilization

The effect of different planting densities (100,000 and 167,000 plants ha^?1) and levels of nitrogen fertilization (0, 261, 521, and 782 kg N ha^?1 year^?1) on biomass production and chemical composition of Moringa oleifera was studied in a split-plot design with four randomized complete blocks over 2 years with eight cuts year^?1 at the National Agrarian University farm in Managua, Nicaragua (12°09′30.65″N, 86°10′06.32″W, altitude 50 m above sea level). Density 167,000 plants ha^?1 produced significantly higher total dry matter yield (TDMY) and fine fraction yield (FFDM), 21.2 and 19.2 ton ha^?1 respectively, compared with 11.6 and 11 ton ha^?1 for 100,000 plants ha^?1. Growth rate in 167,000 plants ha^?1 was higher than in 100,000 plants ha^?1 (0.06 compared with 0.03 ton ha^?1 day^?1). Average plant height was 119 cm irrespective of planting density. Fertilization at the 521 and 782 kg N ha^?1 year^?1 levels produced the highest TDMY and FFDM in both years of the study and along all cuts. The interaction between cut and year was significant, with the highest TDMY and FFDM during the rainy season in the second year. Chemical composition of fractions showed no significant differences between planting densities. Significantly higher crude protein content was found in the coarse fraction at fertilizer levels 521 and 782 kg N ha^?1 year^?1 (87.9 and 93.7 g kg^?1 DM) compared with lower levels. The results indicate that Moringa can maintain up to 27 ton ha^?1 dry matter yield under dry tropical forest conditions over time at a planting density of 167,000 plants ha^?1 if the soil is regularly supplied with N at a level of approximately 521 kg ha year^?1 in conditions where phosphorus and potassium are not limiting.     

Nitrogen fixation and accretion by wax myrtle (Myrica cerifera) in slash pine (Pinus elliottii) plantations

The effects of wax myrtle (Myrica cerifera L.) on the nitrogen cycle were examined in a 23-year-old slash pine (Pinus elliottii Engelm. var. elliottii) plantation located near Gainesville, FL. These shrubs occurred naturally as an understory and had a crown cover of 8% of the study area. The potential rate of nitrogen fixation by wax myrtle was estimated to be 13 g N m^?2 year^?1, or 10.6 kg N ha^?1 year^?1 on a stand wide basis. Wax myrtle fixed substantial amounts of nitrogen throughout the year although winter rates were significantly less due to the greatly reduced activity of old nodules during that season. The average accumulation rate of nitrogen beneath wax myrtle was 1.5 g N m^?2 year^?1 in the soil and 0.9 g N m^?2 year^?1 in the forest floor. On a stand wide basis this amounted to an accretion of 1.9 kg N ha^?1 year^?1 in the soil and forest floor.     

A method for estimating white pine blister rust canker age on limber pine in the central Rocky Mountains

Epidemiological studies of white pine blister rust on limber pine require a temporal component to explain variations in incidence of infection and mortality. Unfortunately, it is not known how long the pathogen has been present at various sites in the central Rocky Mountains of North America. Canker age, computed from canker length and average expansion rate, can be used to estimate infestation origin and infection frequency. To investigate relationships between canker lengths and canker ages for limber pine, we collected live white pine blister rust branch and stem cankers from three locations in Wyoming and two locations in Colorado. We quantified relationships between various measures of canker length and an estimate of canker age based on dendrochronological analysis. Total branch canker length was strongly, negatively correlated (r = ?0.79) with the first year of incomplete, annual ring formation (canker age). Mean longitudinal canker expansion rate was 8.4 cm year^?1 for branch and stem cankers where branches distal to the canker were either dead or alive. Annual longitudinal canker expansion, however, was significantly greater on a stem or branch where the portion distal to the canker was alive (11.5 cm year^?1) rather than dead (7.1 cm year^?1). For branches or stems, proximal expansion rate (i.e., toward or down stem) averaged 4.9 cm year^?1. The circumferential canker expansion rate (around branch or stem) was greater for stem cankers (8.3 cm year^?1) than for branch cankers (6.2 cm year^?1). Additional site and host tree covariates did not improve prediction of canker age. Two simple linear equations were developed to estimate a canker age from total length of a canker with the distal portion either alive or dead. An appropriate sample of canker ages can be used to determine how long a limber pine stand has been infested with white pine blister rust and how frequently infections have occurred.     

Fertilizer type and species composition affect leachate nutrient concentrations in coffee agroecosystems

Intensification of coffee (Coffea arabica) production is associated with increases in inorganic fertilizer application and decreases in species diversity. Both the use of organic fertilizers and the incorporation of trees on farms can, in theory, reduce nutrient loss in comparison with intensified practices. To test this, we measured nutrient concentrations in leachate at 15 and 100 cm depths on working farms. We examined (1) organically managed coffee agroforests (38 kg N ha^?1 year^?1; n = 4), (2) conventionally managed coffee agroforests (96 kg N ha^?1 year^?1; n = 4), and (3) one conventionally managed monoculture coffee farm in Costa Rica (300 kg N ha^?1 year^?1). Concentrations of nitrate (NO₃ ^?-N) and phosphate (PO₄ ^3?-P) were higher in the monoculture compared to agroforests at both depths. Nitrate concentrations were higher in conventional than organic agroforests at 15 cm only. Soil solutions collected under nitrogen (N)-fixing Erythrina poeppigiana had elevated NO₃ ^?-N concentrations at 15 cm compared to Musa acuminata (banana) or Coffea. Total soil N and carbon (C) were also higher under Erythrina. This research shows that both fertilizer type and species affect concentrations of N and P in leachate in coffee agroecosystems.     

Productivity of successive rotations of Acacia mangium plantations in Sumatra,Indonesia: impacts of harvest and inter-rotation site management

The impacts of wood harvest, biomass removal and inter-rotation site management practices on productivity of Acacia mangium in South Sumatra were studied over 12 years across successive rotations. The productivity measured as MAI increased from 29.4 m³ ha^?1 year^?1 in the first to 48.0 m³ ha^?1 year^?1 in the second rotation. Whole tree harvesting (total stem, branches and leaves) caused a 21 % reduction in volume compared to harvesting merchantable wood alone in the next rotation. The rates of nutrients accumulation in trees were highest during the first year of growth, and declined from age 2 years. Significant amounts of nutrients were recycled through litter fall from 1 year after planting. Results highlight the importance of management which promotes nutrient supply on stand growth. Removal of slash and litter lowered soil pH, by about 0.1 unit. A small reduction was also found in soil organic carbon and nitrogen in the top soil during the first 3–4 years but values returned to pre-harvest levels by the end of the rotation. Extractable soil phosphorus and exchangeable cations decreased by the end of second rotation but these measures underestimate the nutrient pools available for A. mangium. These findings along with results from other studies have helped to implement operations which promote conservation of site resources for sustainable production in the region.     

Growth stimulation of young oak plantations inoculated with the ectomycorrhizal fungus Paxillus involutus with special reference to summer drought

Nursery-grown Quercus petraea and Q. robur seedlings were inoculated with the ectomycorrhizal fungi Paxillus involutus, Hebeloma crustuliniforme or Laccaria laccata. At the end of the first growing season, the seedlings with roots colonized with the inoculated fungi were outplanted at two sites in northeastern France in 1985 or 1988. P. involutus was found to be the most competitive and efficient of the three fungi tested; its mycorrhizas were present 7 yrs after outplanting and it markedly improved the growth of both oak species. In the case of Q. robur, the growth stimulation was more marked on years with a dry summer. These results are discussed in terms of water relations and of competitiveness of the introduced versus resident symbiotic fungi.     

High organic carbon stock in a karstic soil of the Middle-European Forest Province persists after centuries-long agroforestry management

Little is known on soil organic carbon (SOC) stocks in karst areas worldwide, although many of them have seen long-term application of agroforestry systems with a potential for carbon sequestration. Therefore, our study aimed to assess landscape-level SOC concentration and stock in the Silica Plateau, a part of the Slovak Karst Biosphere Reserve located in the Western Carpathians (Slovakia) with a centuries-long agroforestry record. The most represented local soil units are Chromi-Rendzic Leptosols and Chromic Cambisols with clayey loam texture, C/N ratio 9–12, and $ {\text{pH}}_{{{\text{H}}_{2} {\text{O}}}} $ 6.6–6.2 in their organo-mineral surface horizons. Mull surface humus form prevails under mixed forest stands dominated by hornbeam (Carpinus betulus L.), oak (Quercus petraea L.), and beech (Fagus sylvatica L.). A total of 2,700 soil samples were collected from 150 soil pits. Both SOC concentrations and stocks were determined for the 0–60?cm mineral soil layer. Soil stoniness was accounted for by means of electrical resistivity tomography. According to the analysis of covariance, cropland SOC concentration (0.026?g?g^?1) is significantly lower compared to forestland (0.040?g?g^?1) and pastureland (0.041?g?g^?1) (P?<?0.01). During the period of 130?years after forest clearing, cropland SOC stock has been reduced at an exponential decay rate of ca 0.002?year^?1, while the SOC stock in pastureland has increased following land use change from cropland by approximately 30% during the same period of time. Irrespective of land use history, overall SOC stock is high reaching on average 207.4?Mg?ha^?1, out of which 66% are stored within 0–30?cm and 34% within 30–60?cm soil layers.     

Conversion of tropical moist forest into cacao agroforest: consequences for carbon pools and annual C sequestration

Tropical forests store a large part of the terrestrial carbon and play a key role in the global carbon (C) cycle. In parts of Southeast Asia, conversion of natural forest to cacao agroforestry systems is an important driver of deforestation, resulting in C losses from biomass and soil to the atmosphere. This case study from Sulawesi, Indonesia, compares natural forest with nearby shaded cacao agroforests for all major above and belowground biomass C pools (n = 6 plots) and net primary production (n = 3 plots). Total biomass (above- and belowground to 250 cm soil depth) in the forest (approx. 150 Mg C ha^?1) was more than eight times higher than in the agroforest (19 Mg C ha^?1). Total net primary production (NPP, above- and belowground) was larger in the forest than in the agroforest (approx. 29 vs. 20 Mg dry matter (DM) ha^?1 year^?1), while wood increment was twice as high in the forest (approx. 6 vs. 3 Mg DM ha^?1 year^?1). The SOC pools to 250 cm depth amounted to 134 and 78 Mg C ha^?1 in the forest and agroforest stands, respectively. Replacement of tropical moist forest by cacao agroforest reduces the biomass C pool by approximately 130 Mg C ha^?1; another 50 Mg C ha^?1 may be released from the soil. Further, the replacement of forest by cacao agroforest also results in a 70–80 % decrease of the annual C sequestration potential due to a significantly smaller stem increment.     

Estimation of biomass,net primary production and net ecosystem production of China's forests based on the 1999–2003 National Forest Inventory

Abstract

The National Forest Inventory (NFI) is an important resource for estimating the national carbon (C) balance. Based on the volume, biomass, annual biomass increment and litterfall of different forest types and the 6th NFI in China, the hyperbolic relationships between them were established and net primary production (NPP) and net ecosystem production (NEP) were estimated accordingly. The results showed that the total biomass, NPP and NEP of China's forests were 5.06 Pg C, 0.68 Pg C year^?1 and 0.21 Pg C year^?1, respectively. The area-weighted mean biomass, NPP and NEP were 35.43 Mg C ha^?1, 4.76 Mg C ha^?1 year^?1 and 1.47 Mg C ha^?1 year^?1 and varied from 13.36 to 79.89 Mg C ha^?1, from 2.13 to 9.15 Mg C ha^?1 year^?1 and from ?0.16 to 5.80 Mg C ha^?1 year^?1, respectively. The carbon sequestration was composed mainly of Betula and Populus forest, subtropical evergreen broadleaved forest and subtropical mixed evergreen–deciduous broadleaved forest, whereas Pinus massoniana forest and P. tabulaeformis forest were carbon sources. This study provides a method to calculate the biomass, NPP and NEP of forest ecosystems using the NFI, and may be useful for evaluating terrestrial carbon balance at regional and global levels.     

Procedure for establishing sheathing mycorrhizas on tree seedlings

A simple procedure is described where seedlings emerging from surface sterilised seeds (of Betula pendula, B. pubescens, Pinus contorta and Picea sitchensis) were grown axenically in a peat/vermiculite mixture which had been previously inoculated, and incubated, with sheating mycorrhizal fungi (including Hebeloma sacchariolens, Paxillus involutus and Amanita muscaria). Mostly, seedlings were grown in split polystyrene tubes (50 mm tall x 13 mm internal diameter) which were arranged $\text{140}\text{150}$ per tray, the trays being covered with transparent propagator lids so forming a unit that could be sealed and sterilised before use with gamma radiation. With the formation of sheating mycorrhizas after about 7 weeks the sterility of the container was broken to enable seedlings to be hardened off prior to transplanting.Roots of Betula pendula seedlings grown in tubes were smaller, as judged by numbers of ‘fragments’, than those of plants which were not ‘restricted’. ‘Tubing’ increased: (a) the proportion of root fragments with mycorrhizas; and (b) the sizes of individual mycorrhizas. The sizes of the ‘tubing’ effects differed with different isolates of A. muscaria, so suggesting, because of the sensitivity of the system, that environmental and genotypic factors must be carefully controlled.     

Biomass and nitrogen dynamics in an irrigated hybrid poplar plantation

A 3-year study measured the effects of ground cover treatments and N fertilization on biomass and nitrogen dynamics in an irridiated hybrid poplar (Populus deltoides Bartr. X P. trichocarpa Torr. and Gray, clone NC-9922) plantation in northern Wisconsin, U.S.A. Annually fertilized (112 kg N ha⁻¹ year⁻¹) and unfertilized plots were maintained weed free (bare soil), allowed to revegetate with native weeds, or seeded to birdsfoot trefoil (Lotus corniculatus L.). Biomass and N in trees and ground-cover vegetation were sampled before and after each growing season.Trees in bare-soil plots responded to fertilization primarily in the third growing season, but total biomass of 3-year-old trees was not increased by annual fertilization. In plots with a ground cover,fertilization increased tree growth but cover crop treatment had no effect. Ground cover biomass peaked during the second growing season, but declined thereafter, primarily due to reductions in below-ground biomass. Estimated recovery of fertilizer N was low in bare soil plots after 3 years, with 2% in the ‘perennial’ portion of the trees and 13% in the leaf litter. In contrast, recovery in the cover crop plots was 44%–51% in years 2–4. During that period, both biomass and N pool dominance shifted from primarily cover crop to primarily trees. The ground cover appeared to reduce tree growth in years 1–3, but total tree biomass after 4 years was greater in fertilized plots with ground cover (22.7 Mg/ha) than in fertilized bare soil plots (16.7 Mg/ha). Biomass production in fertilized trefoil plots in the fourth year (15.1 Mg ha⁻¹ year⁻¹, excluding leaves) exceeds that of local forests by 50%, and may be comparable to corn productivity in the area.     

Forage yield and quality of Leucaena leucocephala and Guazuma ulmifolia in mixed and pure fodder banks systems in Yucatan,Mexico

In tropical areas of Mexico, Leucaena leucocephala is widely used in silvopastoral systems. However, little information exists on other native woody species of high forage potential, such as Guazuma ulmifolia. The aim of this study was to evaluate the components of biomass, forage yield and quality, and availability of N in fodder banks of L. leucocephala, G. ulmifolia, and a mixture of both species during dry and rainy seasons, under sub-humid tropical conditions. The experimental unit was a 5 × 10 m plot, containing three rows with 2 m between rows; each row had 20 plant positions with 0.50 m between plants. Within each plant position there was either a single plant, in the case of pure-crop, or two plants, in the case of mixed of both species. A complete randomized block design with three repetitions was used. In both seasons, there were a significantly greater proportion of leaves in the G. ulmifolia fodder banks (71 %) and in mixed fodder banks (69 %) than in L. leucocephala fodder banks (64 %). Consequently, these systems had leaf-to-stem ratios of 2.4, 2.2 and 1.9, respectively. The forage yield of fodder banks was not influenced by season. The mixed fodder bank had greater forage yield (5.1 t DM ha^?1) than the L. leucocephala fodder bank (3.4 t DM ha^?1) in each season. Additionally, the mixed fodder bank accumulated more forage yield during the experimental period (10.2 t DM ha^?1 year^?1) than G. ulmifolia (9.0 t DM ha^?1 year^?1) or L. leucocephala (6.9 t DM ha^?1 year^?1). The concentrations of CP, C and C:N were not influenced by season. Forage NDF and ADF concentrations were greater in the rainy season (476 g kg^?1 DM) compared with the dry season (325 g kg^?1 DM). Mixed fodder banks had the greatest N yield (185.9 kg ha^?1) and consequently the greatest availability of N (371.8 kg N ha^?1 year^?1). We conclude that mixed fodder banks of L. leucocephala and G. ulmifolia are a better option for improving productivity and forage quality in comparison with pure fodder banks in Yucatan, Mexico.     

Assessing water use and soil water balance of planted native tree species under strong water limitations in Northern Chile

Some forest plantations with native species are established in semiarid central Chile to compensate for industrial activities such as those of mining. Two of those operational forest plantations were monitored from age 1 to 3 years-old (2014–2016). Some plant attributes and soil volumetric water content (VWC) were monitored for eight native tree species (Acacia caven, Schinus polygamus, Porlieria chilensis, Lithraea caustica, Quillaja saponaria, Cryptocarya alba, Drimys winteri and Maytenus boaria), and a water balance model fitted to assess plant water use. Site preparation comprised planting holes of 40 cm?×?40 cm by 50 cm in depth dug with a backhoe. Substrate was removed and mixed with compost in proportion 70:30 before mixing it in the planting hole. Planting holes acted as water reservoirs over the study period with soil VWC generally increasing with soil depth being also less variable deeper than in the upper soil layers. The ratio of adaxial (upper leaf side) to abaxial (lower leaf side) stomatal conductance approximately followed a species gradient from xeric to mesic. Irrigation represented about 26% and 53% of the total water input for the sclerophyll and the D. winteri plantation, respectively. At the plant level (0.4?×?0.4 m), soil evaporation and transpiration of D. winteri (273 and 232 mm year^?1, equivalent to 43.7 and 37.1 L plant^?1, respectively) were about twofold the values for the sclerophyllous/malacophyllous plantation (138 and 128 mm year^?1, 22.1 and 20.5 L plant^?1, respectively). We suggest the water budget for the sclerophyll/malacophyllous plantation was tight but feasible to be adjusted while for D. winteri irrigation was excessive, could be drastically reduced, and suppressed altogether if planted in gullies. We believe water balance models and soil moisture content sensors could be used to better plan and manage irrigation frequency and amounts in compensation forest plantations in semiarid central Chile.     

Red alder leaf decomposition and nutrient release in alder and conifer riparian patches in western Washington,USA

Current management practices encourage conversion of red alder (Alnus rubra) riparian forests to conifers in the Pacific Northwest U.S. Patches of young naturally regenerated conifers are commonly present in alder dominated riparian areas and an understanding of the soil processes in these patches will be helpful in guiding future riparian management. Study objectives were to: (1) determine decomposition rates of red alder leaves in riparian alder and conifer patches, (2) relate decomposition rates to environmental factors and litter chemistry, and (3) determine nutrient release from decomposing alder leaves in these patches. Study sites were riparian areas adjacent to Brown and Le Bar creeks in the Skokomish River basin, Olympic National Forest, Washington. Red alder leaves were placed in litterbags in red alder and conifer riparian patches along each stream in November 2000 and collected after 1 and 3 years. There was rapid mass loss of alder leaves in the first year in both patch types, but decomposition was significantly faster (p < 0.05) in alder patches (43.2% mass remaining, k = 0.855 year⁻¹) than in conifer patches (48.4% mass remaining, k = 0.734 year⁻¹). There was little mass loss after the first year and no significant difference in decomposition rates. After 3 years mass remaining was 44.2% (k = 0.283 year⁻¹) and 47.8% (k = 0.48 year⁻¹) in alder and conifer patches, respectively. Decomposition rate differences were attributed more to the effects of the different litters in each patch and the influence on soil microbial and faunal communities than differences in soil temperature and moisture. The forest floor was deeper in conifer patches (3.7 cm) than alder (1.8 cm) patches. This was ascribed to slower decomposition rates in conifer patches, greater litterfall in conifer patches, and/or removal of alder surface litter by flooding. Alder patches were lower in elevation (0.8 m above bankfull width) than conifer patches (2.2 m). Forest floor and soil C and N concentrations and pHs were not significantly different in alder and conifer patches. Nutrient release from decomposing alder leaves was not significantly different in conifer and alder patches, although there was a trend for C, N, P, K, and Ca to be lost faster from leaves in alder patches than conifer patches in the first year. Red alder litter input to riparian conifer patches will initially decompose rapidly and provide nutrients, particularly N and P to conifers, as well as enhancing soil C since long-term decomposition rates are slow.     

Seedling growth and water relations of three Cedrela species sourced from five provenances: response to simulated rainfall reductions

The effects of different simulated rainfall regimes on water relations, growth, and biomass production and allocation of five provenances of Cedrela fissilis, C. saltensis and C. balansae were assessed in a pot-grown seedling experiment in greenhouse under uncontrolled conditions. Four simulated rainfall regimes were applied: 600 mm year^?1 (severe water deficit), 800 mm year^?1 (mild water deficit), 1,000 mm year^?1 (mild wet), and 1,200 mm year^?1 (well-watered) used as control. Provenances were compared in terms of water relations (midday leaf water potential, Ψ _md and relative water content, RWC), growth (root, shoot and leaf length, neck diameter), growth rate (shoot- and neck growth rate, SGR and NGR), biomass production (root, shoot and leaf dry weight), and biomass allocation (root:shoot ratio, RSR). Multivariate analyses revealed that water relations differed significantly under all simulated rainfall treatments by means of several variables throughout time. Effects of species × treatment, and provenance nested within species × treatment interactions significantly were important. SGR and RSR also were important to explain differences in Ψ _md and RWC between treatments and provenances. Broadly C. balansae and C. saltensis provenances seem to display a better performance than C. fissilis provenances under severe water deficit only. However, despite differences between provenances, it was not possible to clearly separate them according to their origin, based on water relations and growth performance. Even though the results of this study performed on pot-grown seedlings are based on relatively few observations and require additional confirmation, they allow an opportunity for speculation on the factors that are related to intraspecific variations that are likely to occur during the growth of seedlings from different geographical sites in field condition.     

Quantifying biological nitrogen fixation of agroforestry shrub species using 15N dilution techniques under greenhouse conditions

Some land-use systems in Saskatchewan, Canada include the nitrogen-fixing trees buffaloberry (Shepherdia argentea Nutt.), caragana (Caragana arborescens Lam.) and sea buckthorn (Hippophae rhamnoides L.). These species provide various ecological functions such as ameliorating soil moisture, light and temperature but little work has been done quantifying biological nitrogen fixation by these species. Greenhouse experiments were conducted to quantify N₂-fixation using the ¹⁵N natural abundance and the ¹⁵N dilution methods. Buffaloberry failed to form nodules in all but one of the four replicates in the natural abundance experiment. Using the ¹⁵N dilution method, the percentage of N derived from atmosphere (%Ndfa) in the shoot of buffaloberry averaged 64 %. For caragana, the mean  %Ndfa was 59 and 65 % and seabuckthorn was 70 and 73 % measured using the natural abundance and dilution methods, respectively. Because of large variability in biomass production between plants grown in the natural abundance experiment and the dilution experiment, the amounts of N₂ fixed also were very variable. Buffaloberry fixed an average of 0.89 g N m^?2; the average for caragana ranged from 1.14 to 4.12 g N m^?2 and seabuckthorn ranged from 0.85 to 3.77 g N m^?2 in the natural abundance and dilution experiments, respectively. This corresponds to 16 kg N ha^?1 year^?1 for buffaloberry; an average of 15–73 kg N ha^?1 year^?1 in caragana and 11–67 kg N ha^?1 year^?1 in seabuckthorn. The substantial amounts of N₂ fixed by these species indicate that they have the potential to contribute to the overall N balance in land-use systems in which they are included.     

Ectomycorrhizal inoculation of Douglas-fir transplanted container seedlings with commercially produced inoculum

Commercially produced vegetative inocula of Laccaria laccata and Hebeloma crustuliniforme successfully formed ectomycorrhizae with Douglas-fir transplanted container (plug+1) seedlings. After 4.5 months in containers, 83% and 90%, respectively, of short roots were mycorrhizal. L. laccata- or H. crustuliniforme-inoculated seedlings had significantly more mycorrhizal and total short roots than Pisolithus tinctorius-inoculated (4% mycorrhizal root tips) or uninoculated control seedlings. No significant differences were detected in seedling growth at the end of the container phase.After transplantation and growth in nursery beds for 17 months, mean new short root colonization of all seedlings was 80%. H. crustuliniforme persisted as a dominant mycorrhizal fungus on seedlings initially inoculated with this fungus. L. laccata-inoculated seedlings had 40% of their short roots colonized by L. laccata and another 40% by native fungi Rhizopogon and Thelephora spp. All mycorrhizae of control seedlings and those inoculated with P. tinctorius were formed by fungi native to the nusery beds. A significant fungal treatment effect was detected for shoot height only. Control seedlings were significantly taller than L. laccata-inoculated seedlings after transplanting.This article is part of senior author's thesis in partial fulfillment of the Ph.D. degree in the Department of Forest Science at Oregon State University, Corvallis.     

Diversity of ectomycorrhizal fungus assemblages on nursery grown European beech seedlings

? Context

An important feature of forest nursery management is the production of first-rate planting stock, which is closely connected with an adequate development of ectomycorrhizas on seedling roots.

? Aims

The aim of this study was to provide a detailed assessment of the ectomycorrhizal (ECM) fungal diversity of European beech grown in bare-root forest nursery conditions.

? Methods

Morphological and molecular analyzes were used to identify ECM fungi, quantify fungal richness, and record differences in the relative abundance of individual taxa.

? Results

Twenty-seven different mycorrhizal fungal taxa were found in four tested nurseries. Of these 27, only 6 were present in more than one nursery. Taxa richness in individual nurseries ranged from 6 to 13, indicating that ECM colonization potential is nursery-dependent. The most frequent taxa were: Tuber sp. 1, Cadophora sp., Cenococcum geophilum agg., Hebeloma sacchariolens, and Laccaria tortilis. According to the analysis of similarity, ECM fungal communities were significantly different between nurseries (R?=?0.7741; p?<?0.0001).

? Conclusion

Beech seedlings grown in bare-root nurseries are colonized by a highly diverse suite of ECM fungi; thus, artificial inoculation of seedlings is generally not necessary in nursery practice.