Influence of soil type and indigenous pathogenic fungi on bean hypocotyl rot caused by Rhizoctonia solani AG4 HGI in Cuba

Calcisol, ferralsol and vertisol soils, representative of different bean production areas of Villa Clara province in Cuba, were selected to determine the impact of soil type on bean hypocotyl rot severity caused by Rhizoctonia solani AG4 HGI (isolate CuVC-Rs7). In inoculated autoclaved soil, hypocotyl rot was most severe in calcisol soil, followed by ferralsol soils and then vertisol soils. In inoculated natural soils, disease severity was lower in vertisol and calcisol soils and higher in ferralsol soil, indicating that biological factors are suppressing or stimulating the pathogenic efficiency of R. solani. Native binucleate Rhizoctonia AGF, Sclerotium rolfsii and R. solani AG 4 HGI were isolated from bean plants grown in natural calcisol, vertisol and ferralsol soils, respectively. Subsequent studies about the interaction between these fungi and R. solani indicated that they were involved in the variability of disease severity caused by R. solani. The addition of R. solani AG4 HGI (isolate CuVC-Rs7) into each autoclaved soil inoculated with binucleate Rhizoctonia or S. rolfsii resulted in a reduction of disease severity caused by this pathogen while in soils inoculated with native R. solani AG4 HGI, disease severity increased. Irrespective of fungal interactions, calcisol was always the most disease conducive soil and vertisol the most disease repressive soil. The mechanisms by which native pathogenic fungi could influence disease severity caused by R. solani are discussed.     

Effect of biochar amendment on soil physical,chemical and biological properties and groundnut yield in rainfed Alfisol of semi-arid tropics

The aim of this study was to evaluate the effect of biochar and organic soil amendments on soil physicochemical and microbial load, carbon sequestration potential, nutrient uptake and yield of groundnut in acidic red soil under rainfed condition. Biochar was prepared from red gram, cotton, maize stalk and mesquite wood using pilot scale slow pyrolysis biochar unit. The above sources of biochar at the rate of 2.5 and 5 t ha^?1 and enriched farmyard manure 0.75 t ha^?1, composted coir pith 10 t ha^?1 and arbuscular mycorrhizae 100 kg ha^?1 were applied as basal with required nitrogen, phosphorous and potassium fertilizer. Biochar amendment at the rate of 5 t ha^?1 reduced the bulk density from 1.41 to 1.36 g cm^?3 and increased the soil moisture 2.5%. With respect to soil chemical changes, it raised soil pH from 5.7 to 6.3; increased the cation exchange capacity 1.4 cmol⁺ kg^?1 and enhanced the carbon buildup 4.4 t ha^?1. The significant differences in bacteria, fungi and actinomycetes population were observed between biochar and control. The nitrogen, phosphorous and potassium were better utilized under biochar and composted coir pith, which was 21, 5 and 20 kg ha^?1 higher than control. The experimental results suggested that application of biochar to acidic red soil favoured good soil physical, chemical and biological environment, and these positive changes influenced growth and yield attributes and enhanced pod yield 29% over control.     

Wheat straw and its biochar had contrasting effects on soil C and N cycling two growing seasons after addition to a Black Chernozemic soil planted to barley

Application of crop residues and its biochar produced through slow pyrolysis can potentially increase carbon (C) sequestration in agricultural production systems. The impact of crop residue and its biochar addition on greenhouse gas emission rates and the associated changes of soil gross N transformation rates in agricultural soils are poorly understood. We evaluated the effect of wheat straw and its biochar applied to a Black Chernozemic soil planted to barley, two growing seasons or 15 months (at the full-bloom stage of barley in the second growing season) after their field application, on CO₂ and N₂O emission rates, soil inorganic N and soil gross N transformation rates in a laboratory incubation experiment. Gross N transformation rates were studied using the ¹⁵N isotope pool dilution method. The field experiment included four treatments: control, addition of wheat straw (30 t ha^?1), addition of biochar pyrolyzed from wheat straw (20 t ha^?1), and addition of wheat straw plus its biochar (30 t ha^?1 wheat straw + 20 t ha^?1 biochar). Fifteen months after their application, wheat straw and its biochar addition increased soil total organic C concentrations (p?=?0.039 and <0.001, respectively) but did not affect soil dissolved organic C, total N and NH₄ ⁺-N concentrations, and soil pH. Biochar addition increased soil NO₃ ^?-N concentrations (p?=?0.004). Soil CO₂ and N₂O emission rates were increased by 40 (p?p?=?0.03), respectively, after wheat straw addition, but were not affected by biochar application. Straw and its biochar addition did not affect gross and net N mineralization rates or net nitrification rates. However, biochar addition doubled gross nitrification rates relative to the control (p?2 and N₂O emissions and enhance soil C sequestration. However, the implications of the increased soil gross nitrification rate and NO₃ ^?-N in the biochar addition treatment for long-term NO₃ ^?-N dynamics and N₂O emissions need to be further studied.     

Effect of biochar and phosphorus fertilizer application on soil fertility: soil physical and chemical properties

This study evaluated the effect of biochar and phosphorus fertilizer application on selected soil physical and chemical properties in two contrasting soil types: Rhodic Ferralsols (clay) in Thohoyandou and Leptic Cambisols (loamy sand) in Nelspruit, South Africa. Field experiments were conducted in summer and winter. Treatments consisted of a factorial combination of four biochar levels (0, 5, 10 and 20 t ha^?1) and two phosphorus fertilizer levels (0 and 90 kg ha^?1) arranged in a randomized complete block design with three replicates. Chickpea was the test crop. Soil bulk density, aggregate stability, porosity, total C, total N, C:N ratio, K and Mg were determined. Biochar (10 t ha^?1) and phosphorus increased bulk density and decreased porosity at 0–5 and 15–20 cm soil depth on a loamy sand soil in both seasons. The interaction between biochar and phosphorus increased total C and total N on a clay soil in the summer sowing. However, in the loamy sand soil, biochar (10 t ha^?1) increased total C, C:N ratio, K and Mg in the summer sowing. The effect of biochar was more evident in the loamy sand soil than the clay soil suggesting that the influence of biochar may be soil-specific.     

Short-term effects of organo-mineral biochar and organic fertilisers on nitrogen cycling,plant photosynthesis,and nitrogen use efficiency

Purpose

Organo-mineral biochar fertiliser has the potential to replace conventional biochar and organic fertiliser to improve soil quality and increase plant photosynthesis. This study explored mechanisms involved in nitrogen (N) cycling in both soil and ginger plants (Zingiber officinale: Zingiberaceae) following different treatments including organic fertiliser, commercial bamboo biochar fertiliser, and organo-mineral biochar fertiliser.

Materials and methods

Soil received four treatments including (1) commercial organic fertiliser (5 t ha^?1) as the control, (2) commercial bamboo biochar fertiliser (5 t ha^?1), (3) organo-mineral biochar fertiliser at a low rate (3 t ha^?1), and (4) organo-mineral biochar fertiliser at a high rate (7.5 t ha^?1). C and N fractions of soil and plant, and gas exchange measurements were analysed.

Results and discussion

Initially, organo-mineral biochar fertiliser applied at the low rate increased leaf N. Organo-mineral biochar fertiliser applied at the high rate significantly increased N use efficiency (NUE) of the aboveground biomass compared with other treatments and improved photosynthesis compared with the control. There was N fractionation during plant N uptake and assimilation since the ¹⁵N enrichment between the root, leaf, and stem were significantly different from zero; however, treatments did not affect this N fractionation.

Conclusions

Organo-mineral biochar fertiliser has agronomic advantages over inorganic and raw organic (manure-based) N fertiliser because it allows farmer to put high concentrations of nutrients into soil without restricting N availability, N uptake, and plant photosynthesis. We recommend applying the low rate of organo-mineral biochar fertiliser as a substitute for commercial organic fertiliser.

     

Soil management for raising crop water productivity in rainfed production systems in Lao PDR

This study investigated the impacts of organic- and clay-based soil amendments, and their combinations on crop water productivity (CWP) using maize as a test crop. On-station field trials were established over two consecutive years at the Naphok and Veunkham sites in Laos. At each site, 10 treatments were applied in a randomized complete block design with three replications. The treatments were control, rice husk biochar (10 t ha^?1), bentonite clay (10 t ha^?1), compost (4 t ha^?1), clay-manure compost (10 t ha^?1), rice husk biochar compost (10 t ha^?1), bentonite clay + biochar, bentonite-clay + compost, biochar + compost, and bentonite clay + biochar + compost. All treatments were applied in 2011. Significant (p < 0.05) treatment effects in CWP and growing period evapotranspiration were determined. At Naphok, differences between the amended and control plots in CWP varied between 0.1 and 0.6 kg m^?3 in 2011 and from 0.1 to 0.4 kg m^?3 in 2012, whereas differences at Veunkham varied between 0.3 and 1.0 kg m^?3 in 2011 and from 0.05 to 0.29 kg m^?3 in 2012. At both sites, CWP in 2012 was significantly lower than 2011. Our results illustrate that organic- and clay-based soil amendments improve CWP, indicating that soil-based interventions could be suitable options for improving agricultural productivity.     

Biochar and fertiliser applications influence phosphorus fractionation and wheat yield

The use of biochar in agriculture to achieve the dual benefits of improving soil quality whilst sequestering carbon (C) has received much attention. However, in low-intensity broadacre agricultural systems where yield is constrained by rainfall and costs associated with phosphorus (P) fertiliser, the application of biochar at rates commonly reported (>10 t ha^?1) are likely to be prohibitively expensive where yield benefits cannot be guaranteed. In marginal areas where calcareous soils dominate, biochar application has no liming effect, reducing its value compared to application in acidic soils. In the present study, we use a field experiment to investigate the interaction between P fertilisation and biochar banding at low application rates (<1 t ha^?1) on wheat yield and soil P fractionation (assessed by a modified Hedley method) in a highly alkaline Haplic Calcisol in a dryland broadacre cropping system. Our results demonstrate no statistically significant effect of low rate biochar banding on wheat yield in this highly P-constrained soil, but a significant effect of both biochar and fertiliser on P fractionation in both years of the study. Higher P fertiliser rates significantly increased wheat yield in all biochar treatments. The interactions between biochar, P fertiliser and P fractionation indicate shifts in potential P availability both as a result of P fertilisation and also biochar application. Further work is required in low productivity calcareous systems such as that studied here to elucidate the potential for biochar amendment to improve productivity and sequester C.     

Biochar amendment changes temperature sensitivity of soil respiration and composition of microbial communities 3 years after incorporation in an organic carbon-poor dry cropland soil

Topsoil samples were collected from plots in a dry cropland in the North China Plain 3 years after a single incorporation of biochar at 20 and 40 t ha^?1 and analyzed for abundances and composition of microbial community and for respiration under controlled laboratory conditions at 15, 20, and 25 °C. The addition of biochar generally reduced soil respirations at the three temperatures and the temperature sensitivity (Q₁₀) at 15–20 °C. Biochar amendment significantly increased bacterial 16S rRNA gene abundances and fungal ITS gene diversity and induced clear changes in their community compositions due to improvements in soil chemical properties such as soil organic C (SOC) and available N contents and pH. Illumina Miseq sequencing showed that the relative abundances of Actinobacteria, Gammaproteobacteria, Firmicutes, and Alternaria within Ascomycota, capable of decomposing SOC, were significantly decreased under biochar at 40 t ha^?1. The Q₁₀ values at 15–20 °C were significantly correlated with fungal diversity and dehydrogenase activity. Our results suggest that after 3 years a single biochar amendment could induce a shift in microbial community composition and functioning towards a slower organic C turnover and stability to warming, which may potentially reduce soil C loss in dryland under climate warming in the future.     

Changes in δ15N in a soil–plant system under different biochar feedstocks and application rates

The application of biochar in soils has been hypothesised to improve soil quality whilst enhancing carbon (C) sequestration. However, its effect on nitrogen (N) dynamics in the soil–plant system is still not fully understood. In the present work, N isotope composition (δ¹⁵N) was used to facilitate the understanding of the processes involved in the N cycling when biochar is applied. We evaluated, through a wheat pot trial, the effect of different application rates of two types of biochar produced from jarrah and pine woodchips on the wheat biomass at harvest and on the soil and plant C and N contents and δ¹⁵N. In addition, the potential benefit of using nutrient-saturated biochar for the soil–plant system was also investigated. Whilst biochar produced from different feedstocks had similar effects on soil and plant nutrient contents, they induced differences in wheat grain biomass and plant δ¹⁵N. The effect of the biochar application rate was more pronounced, and at rates higher than 29 t ha^?1, the application of biochar decreased grain biomass by up to 39 % and potentially increased N losses. Isotopic analyses indicated that this acceleration of N dynamics had probably occurred before the stage of wheat grain formation. The application of nutrient-enriched biochar resulted in an improved wheat grain production, most likely due to the enhanced nutrient availability, and in reduced N cycling rates in the plant–soil system, which could offset the competition between biochar and plants for nutrients and could decrease adverse environmental impacts due to N losses.     

Growth,yield and water use efficiency of chickpea (Cicer arietinum): response to biochar and phosphorus fertilizer application

Field experiments were conducted during summer (2013/2014) and winter (2014) in two different soil types to evaluate the effect of biochar and P fertilizer application on growth, yield, and water use efficiency of chickpea. Soil types include Rhodic Ferralsols (clay) in Thohoyandou and Leptic Cambisols (loamy sand) in Nelspruit, South Africa. Treatments consisted of a factorial combination of four biochar levels (0, 5, 10 and 20 t ha^?1) and two phosphorus fertilizer levels (0 and 90 kg ha^?1) arranged in a randomized complete block design and replicated three times. Biochar application at 5 t ha^?1 significantly increased biomass, grain yield and water use efficiency of biomass production (WUE_b) in the clay soil compared to 10 and 20 t ha^?1. However, the increase was attributed to the addition of P fertilizer. Biochar application had no effect on yield components in the loamy sand soil, but P fertilizer addition increased number of seeds/pod in the loamy sand soil and number of pods/plant in the clay soil. Biochar and P fertilizer application on growth and yield of chickpea varied in soil types and seasons, as the effect was more prominent in the clay soil than the loamy sand soil during the summer sowing.     

Biochar Application Promotes Growth Parameters of Soybean and Reduces the Growth Difference

Biochar is derived from the pyrolysis of biomass, and when buried in soil, can act as a long-term soil carbon store. Evidence suggests that biochar can improve soil fertility and crop production in some circumstances. However, the potential for promoting crop growth in agroecosystems is poorly understood, with different reports of soil properties following biochar application. Therefore, this study aims to investigate the spatial variability of soybean growth parameters in different biochar application rates and the reasons for the results obtained. The study used field plots with five biochar application rates (0, 20,40, 80, and 160 t·ha^?1). There were 15 field plots in total and the trial was fully randomized with three replications. The biochar was mixed into the topsoil (0–20 cm depth) before the soybean was sown. Soybean growth parameters were determined during the flowering–podding phase. The results showed that biochar not only improved the growth parameters of soybean, but also reduced growth differences in the same treatment, and higher biochar application rates would result in a larger effect size in a certain range (0–103.4 t·ha^?1). The leaf area in the no biochar treatment showed moderate variation, whereas the growth parameters (height, stem diameter, and leaf area) showed weak variation in the other biochar treatments; and the growth parameters, when the soybean were subjected to the biochar treatments, had moderate spatial dependence. In addition, the introduction of geostatistical theory can provide a theoretical foundation for further studying biochar–soil–crop system and the spatial variability of soybean growth and their relevance.     

Effect of biochar on aerobic processes,enzyme activity,and crop yields in two sandy loam soils

Biochar added to agricultural soils may sequester carbon and improve physico-chemical conditions for crop growth, due to effects such as increased water and nutrient retention in the root zone. The effects of biochar on soil microbiological properties are less certain. We addressed the effects of wood-based biochar on soil respiration, water contents, potential ammonia oxidation (PAO), arylsulfatase activity (ASA), and crop yields at two temperate sandy loam soils under realistic field conditions. In situ soil respiration, PAO, and ASA were not significantly different in quadruplicate field plots with or without biochar (20 Mg ha^?1); however, in the same plots, volumetric water contents increased by 7.5 % due to biochar (P?=?0.007). Crop yields (oat) were not significantly different in the first year after biochar application, but in the second year, total yields of spring barley increased by 11 % (P??1, applied during two consecutive years, substantiated that biochar was not inhibitory to PAO and ASA as reference plots consistently showed lowest activities. For PAO, it was found that soil pH, rather than biochar rates, was a driving environmental variable. For ASA, the methodological approach was challenged by product sorption, but results did not suggest that biochar significantly stimulated the enzyme activity. Crop yields of maize in field experiments with 10–100 Mg biochar ha^?1 were unaffected by biochar except for a negative effect of the highest annual rates of 50 Mg ha^?1 in the first year after application. In conclusion, the present wood-based biochar poorly affected the measured microbial processes and generally resulted in similar crop yields in reference and biochar-amended soil plots.     

Effects of cotton (Gossypium hirsutum L.) straw and its biochar application on NH3 volatilization and N use efficiency in a drip-irrigated cotton field

Reducing ammonia (NH₃) volatilization is a practical way to increase nitrogen (N) fertilizer use efficiency (NUE). In this field study, soil was amended once with either cotton (Gossypium hirsutum L.) straw (6 t ha^?1) or its biochar (3.7 t ha^?1) unfertilized (0 kg N ha^?1) or fertilized (450 kg N ha^?1), and then soil inorganic N concentration and distribution, NH₃ volatilization, cotton yield and NUE were measured during the next two growing seasons. In unfertilized plots, NH₃ volatilization losses in the straw-amended and biochar-amended treatments were 38–40% and 42–46%, respectively, less than that in control (i.e., unamended soil) during the two growing seasons. In the fertilized plots, NH₃ volatilization losses in the straw-amended and biochar-amended treatments were 30–39% and 43–54%, respectively, less than that in the control. Straw amendment increased inorganic N concentrations, cotton yield, cotton N uptake and NUE during the first cropping season after application, but not during the second. In contrast, biochar increased cotton N uptake and NUE during both the first and the second cropping seasons after application. Furthermore, the effects of biochar on cotton N uptake and NUE were greater in the second year than in the first year. These results indicate that cotton straw and cotton straw biochar can both reduce NH₃ volatilization and also increase cotton yield, N uptake and NUE. In addition, the positive effects of one application of cotton straw biochar were more long-lasting than those of cotton straw.     

Biochars immobilize soil cadmium, but do not improve growth of emergent wetland species Juncus subsecundus in cadmium-contaminated soil

Purpose

An addition of biochar mixed into the substrate of constructed wetlands may alleviate toxicity of metals such as cadmium (Cd) to emergent wetland plants, leading to a better performance in terms of pollutant removal from wastewater. The objective of this study was to investigate the impact of biochars on soil Cd immobilization and phytoavailability, growth of plants, and Cd concentration, accumulation, and translocation in plant tissues in Cd-contaminated soils under waterlogged conditions.

Materials and methods

A glasshouse experiment was conducted to investigate the effect of biochars derived from different organic sources (pyrolysis of oil mallee plants or wheat chaff at 550 °C) with varied application amounts (0, 0.5, and 5 % w/w) on mitigating Cd (0, 10, and 50 mg kg^?1) toxicity to Juncus subsecundus under waterlogged soil condition. Soil pH and CaCl₂/EDTA-extractable soil Cd were determined before and after plant growth. Plant shoot number and height were monitored during the experiment. The total root length and dry weight of aboveground and belowground tissues were recorded. The concentration of Cd in plant tissues was determined.

Results and discussion

After 3 weeks of soil incubation, pH increased and CaCl₂-extractable Cd decreased significantly with biochar additions. After 9 weeks of plant growth, biochar additions significantly increased soil pH and electrical conductivity and reduced CaCl₂-extractable Cd. EDTA-extractable soil Cd significantly decreased with biochar additions (except for oil mallee biochar at the low application rate) in the high-Cd treatment, but not in the low-Cd treatment. Growth and biomass significantly decreased with Cd additions, and biochar additions did not significantly improve plant growth regardless of biochar type or application rate. The concentration, accumulation, and translocation of Cd in plants were significantly influenced by the interaction of Cd and biochar treatments. The addition of biochars reduced Cd accumulation, but less so Cd translocation in plants, at least in the low-Cd-contaminated soils.

Conclusions

Biochars immobilized soil Cd, but did not improve growth of the emergent wetland plant species at the early growth stage, probably due to the interaction between biochars and waterlogged environment. Further study is needed to elucidate the underlying mechanisms.     

Effects of biochar and fertilizer management on sunflower (Helianthus annuus L.) feedstock and soil properties

The need for bioenergy is increasing with increase in global energy demand, and sustainable soil and fertilizer management practices for bioenergy feedstock production are gaining importance. In this greenhouse study, we evaluated the effects of biochar and fertilizer nitrogen on soil and energy crop sunflower (Helianthus annuus L. var. Giganteus). Sunflower plants were treated with three rates of biochar, control (0 Mg ha^?1), low (25 Mg ha^?1) and high (50 Mg ha^?1), and three rates of fertilizers, 0% (control), 50% (low) and 100% (high) of the recommended nitrogen dose. Plant height, quality (chlorophyll content), biomass yield, feedstock energy, ash content and tissue nutrients were measured along with soil moisture and pH. Results showed an 11% increase in mean plant height under low biochar compared to control biochar-treated plants. High nitrogen treatment produced 26% and 18% more stalk and total above-ground plant (whole plant) biomass, respectively, compared to the control nitrogen treatment. High biochar treatment resulted in higher soil moisture holding, but lower soil pH than the control biochar treatment. Plant quality, energy and ash contents were not affected by either biochar or nitrogen. The plant tissue analysis provides a complete tissue macro- and micronutrient information on sunflower cultivar Giganteus, which was not done previously.     

Effect of biochar and nitrapyrin on nitrous oxide and nitric oxide emissions from a sandy loam soil cropped to maize

A field experiment was conducted to evaluate the combined or individual effects of biochar and nitrapyrin (a nitrification inhibitor) on N₂O and NO emissions from a sandy loam soil cropped to maize. The study included nine treatments: addition of urea alone or combined with nitrapyrin to soils that had been amended with biochar at 0, 3, 6, and 12 t ha^?1 in the preceding year, and a control without the addition of N fertilizer. Peaks in N₂O and NO flux occurred simultaneously following fertilizer application and intense rainfall events, and the peak of NO flux was much higher than that of N₂O following application of basal fertilizer. Mean emission ratios of NO/N₂O ranged from 1.11 to 1.72, suggesting that N₂O was primarily derived from nitrification. Cumulative N₂O and NO emissions were 1.00 kg N₂O-N ha^?1 and 1.39 kg NO-N ha^?1 in the N treatment, respectively, decreasing to 0.81–0.85 kg N₂O-N ha^?1 and 1.31–1.35 kg NO-N ha^?1 in the biochar amended soils, respectively, while there was no significant difference among the treatments. NO emissions were significantly lower in the nitrapyrin treatments than in the N fertilization-alone treatments (P?<?0.05), but there was no effect on N₂O emissions. Neither biochar nor nitrapyrin amendment affected maize yield or N uptake. Overall, our results showed that biochar amendment in the preceding year had little effect on N₂O and NO emissions in the following year, while the nitrapyrin decreased NO, but not N₂O emissions, probably due to suppression of denitrification caused by the low soil moisture content.     

Effect of Municipal Solid Waste Compost and Farmyard Manure Application on Heavy‐Metal Uptake in Wheat

The risks related to municipal solid waste compost application in comparison to farmyard manure and mineral fertilizers on durum wheat were investigated on a short‐term experiment. Compost was applied at 40 t ha^?1 and 80 t ha^?1 with or without chemical fertilizers. Analogously, farmyard manure was applied at 40 t ha^?1. Both compost and farmyard manure improved plant growth and nutrient uptake. However, compost amendment showed more effectiveness, especially at 80 t ha^?1. Alternatively, this dose of compost involved an increase of plant copper, cadmium, and zinc concentrations in plant tissues. Metal accumulation did not thwart the enhancement of wheat yield. Furthermore, grain translocation factor reached 1 only in the case of copper; however, it showed a significant decrease following compost application (ranged between 0.57 and 0.69). Bioconcentration factor showed a significant decrease with municipal solid waste compost supply, constituting an internal detoxification mechanism.     

Biochar and inorganic nitrogen fertilizer effects on maize (Zea mays L.) nitrogen use and yield in moist semi-deciduous forest zone of Ghana

Abstract

Maize (Zea mays L.) is a major nitrogen consuming crop, as nitrogen is considered as an important determinant of its grain yield. Though inorganic fertilizer is widely recommended, the problem of high cost and inaccessibility limit its usage by resource poor farmers. Biochar application provides a new technology for both soil fertility and crop productivity improvement. With limited research on the suitability of biochar for soil improvement practices in Ghana, our objective was to determine the synergistic effect of biochar and inorganic fertilizer on the nitrogen uptake, nitrogen use efficiency, and yield of maize. Field experiment was conducted in Ghana, KNUST, in the major and minor raining seasons. Biochar was applied at 0, 5, 10, 15, and 20 t ha^?1 and fertilizer N applied at 0, 45, and 90?kg ha^?1. The results showed significantly (p??1 supplemented with 45?kg N ha^?1 increased N uptake by 200%, and grain yield by 213% and 160% relative to the control in the minor and major rainy seasons, respectively. The greater yield of maize recorded on biochar-amended soils was attributed to the improved N uptake and nitrogen use efficiency. In conclusion, our finding suggests that the application of combined biochar and inorganic N fertilizer is not only ecologically prudent, but economically viable and a practicable alternative to current farmers’ practice of cultivating maize in Ghana.     

Retention and release of diethyl phthalate in biochar-amended vegetable garden soils

Purpose

Diethyl phthalate (DEP) is one of the most commonly used plasticizers as well as a soil contaminant. Using biochar to remediate soils contaminated with DEP can potentially reduce the bioavailability of DEP and improve soil properties. Therefore, a laboratory study was conducted to evaluate the effect of biochar on soil adsorption and desorption of DEP.

Materials and methods

Two surface soils (0–20 cm) with contrasting organic carbon (OC) contents were collected from a vegetable garden. Biochars were derived from bamboo (BB) and rice straw (SB) that were pyrolyzed at 350 and 650 °C. Biochars were added to two types of soil at rates of 0.1 and 0.5 % (w/w). A batch equilibration method was used to measure DEP adsorption-desorption in biochar treated and untreated soils at 25 °C. The adsorption and desorption isotherms of DEP in the soils with or without biochar were evaluated using the Freundlich model.

Results and discussion

The biochar treatments significantly enhanced the soil adsorption of DEP. Compared to the untreated low organic matter soil, the soils treated with 0.5 % 650BB increased the adsorption by more than 19,000 times. For the straw biochar treated soils, the increase of DEP adsorption followed the order 350SB?>?650SB. However, for the bamboo biochars, the order was 650BB?>?350BB. Bamboo biochars were more effective than the straw biochars in improving soils’ adsorption capacity and reducing the desorption ability of DEP.

Conclusions

Adding biochar to soil can significantly enhance soil’s adsorption capacity on DEP. The 650BB amended soil showed the highest adsorption capacity for DEP. The native soil OC contents had significant effects on the soils’ sorption capacity treated with 650BB, whereas they had negligible effects on the other biochar treatments. The sorption capacity was affected by many factors such as the feedstock materials and pyrolysis temperature of biochars, the pH value of biochar, and the soil organic carbon levels.     

Assessment of Environment-friendly Usage of Spent Wash and its Nutritional Potential for Sugarcane Production

Excessive and inappropriate use of fertilizers is a key factor of low sugarcane yield and degradation of soil. A two-year (2013–14 and 2014–15) field study was conducted to assess the impact of combined application of organic and inorganic fertilizers on sugarcane at research farm of Shakarganj Sugar Research Institute, Jhang, Pakistan. Experiment was conducted under randomized complete block design with three replications. Treatments were used as control (no exogenous application), spent wash (160 t ha^?1), (nitrogen, phosphorus and potassium) NPK (168:112:112 kg ha^?1), spent wash (120 t ha^?1) + NPK (42:28:28 kg ha^?1), spent wash (80 t ha^?1) + NPK (84:56:56 kg ha^?1), spent wash (40 t ha^?1) + NPK (126:84:84 kg ha^?1), and spent wash (160 t ha^?1) + NPK (42:28:28 kg ha^?1). Application of spent wash @ 80 t ha^?1 + NPK @ 84:56:56 kg ha^?1 resulted maximum crop growth rate (11.35 g m^?2 d^?1), leaf area index (7.78), and net assimilation rate (2.53 g m^?2 d^?1). Maximum number of millable canes (14), weight per stripped cane (0.90 kg), stripped cane yield (117.60 t ha^?1) and unstripped cane yield (141.25 t ha^?1) were observed with spent wash @ 80 t ha^?1 + NPK @ 84:56:56 kg ha^?1, followed by sole fertilizer application @ 168:112:112 kg NPK ha^?1 and spent wash @160 t ha^?1 + NPK @ 42:28:28 kg ha^?1. Similar trend was observed regarding quality parameters. The maximum benefit–cost ratio (1.80) was achieved with integrated application of spent wash @ 80 t ha^?1 + NPK @ 84:56:56 kg ha^?1.