Effects of tillage,nitrogen placement,and wheel compaction on denitrification rates in the corn cycle of a corn‐oats rotation

Denitrification rates under various tillage systems were determined in the corn (Zea mays L.) cycle of a corn‐oats (Avena sativa L.) rotation. Denitrification was measured directly with an in‐situ soil cover method which supplied the soil with acetylene (C₂H₂) and evacuated the nitrous oxide (N₂O) produced. Denitrification rates were measured in both a field or non‐wheel track (NWT) area and in a compacted wheel track (WT) area for the no‐till (NT), chisel plow (CH), moldboard plow (MP) tillage systems after nitrogen (N) was applied by broadcast/incorporation with 112 kg N/ha as ammonium nitrate. Nitrogen was also applied to the NT treatment by injection with modified anhydrous ammonia knives prior to planting. Most of the cumulative N loss occurred over a 22 day period following heavy rainfall in June. Denitrification was greatest on days after rainfall events for the NT systems. Cumulative N loss was estimated at 25, 16, and 11 kg N/ha from May 29‐September 8 for NT, CH, and MP treatments, respectively, for the broadcast/incorporated N application. Mean denitrification rates from WT areas were about 1.6 times greater than the NWT areas.     

Effects of divalent cations on nadh‐linked electron transfer in corn root plasma membrane

The effects of calcium (Ca²⁺), cadmium (Cd²⁺), and copper (Cu²⁺) cations on NADH‐linked electron transfer in com root plasma membrane vesicles were investigated. The reduction of both cytochrome c and ferricyanide were slightly stimulated by Ca²⁺ but not significantly affected by Cd²⁺. However, Cd²⁺ induced a redox‐linked increase in light scattering suggesting an increase in the size/volume of the vesicles. The presence of micromolar levels of Cu²⁺ decreased the reduction rates of both cytochrome c and ferricyanide. However, in contrast to ferricyanide reduction, Cu inhibition to the cytochrome c reduction was more effective, and was less sensitive to ionic strength. Copper inhibition changed the Michaelis‐Menten dependence of the ferricyanide reduction but not that of cytochrome c. These results suggest that the reduction of cytochrome c. and ferricyanide must occur at different membrane sites.     

Nitrogen and Irrigation Effects on β-Glucan Content of Wheat Grain

g -glucan is a major source of dietary fiber and a water-soluble fiber component of wheat grain. The objective of this study was to examine the effects of different nitrogen and irrigation levels on g -glucan content of wheat grain in the years 1998-2000. Experimental design was a split-split, randomized complete block with three replications. Three irrigation levels (0, 75 and 150 mm) and nitrogen rates (0, 50 and 100 kg/ha) were applied in three winter wheat cultivars. Increased levels of applied nitrogen tended to increase grain g -glucan content in 1999. Non-irrigated (I 0 ) cultivars gave the highest g -glucan contents in all treatments (2000). The mean g -glucan values of Bezostaja 1, Gün 91 and Ikizce 96 were 0.615, 0.391 and 0.361% respectively in 1999. In 2000, the mean g -glucan contents of Bezostaja 1, Gün 91 and Ikizce 96 were 0.704, 0.492 and 0.376% respectively. Correlations between g -glucan content and grain yield, protein content, thousand grain weight and gluten content were significant in both years. The results of this study indicated that cultivars and increasing nitrogen levels are important in determining wheat grain g -glucan content, while irrigation decreased grain g -glucan content.     

Effects of supplemental‐nitrogen on the quality of rice proteins

A study was conducted on the effect of supplemental nitrogen (N) (20 hg/ha) applied as a foliar spray or to the soil on seed production, protein percentage, and protein fractions of rice. Plants were grown in a greenhouse over two different periods of time, i.e., August 1988 to January 1989 (Period I), and December 1988 to April 1989 (Period II). Nitrogen was applied to the leaves 10 and 20 days after anthesis (DAA), and to the soil at anthesis and at 15 DAA. Average temperature was 28.7°C during Period I and 32°C during Period II, corresponding to 18.7 and 22.0 growing degree‐day/day (GDD/day), respectively. The difference in GDD/day reduced the plant cycle from 130 days during Period I to 109 days during Period II. Plants grown during Period II had larger numbers of spikelets, a higher percentage of “full grown grains”;, and higher grain weight. Although percentage crude protein was about the same for the two periods, prolamin content was increased and the albumin+globulin fraction was decreased during Period II, but with no difference in glutelin content. The increase in number of spikelets, percent full grains, and grain weight appeared to result in a greater energy demand for plants grown during Period II. This may explain the increase in prolamins, since prolamin synthesis requires less energy than globulin or albumin synthesis. There was a simultaneous decrease in albumin and globulin synthesis during Period II. The content of glutelins, which represent the major reserve proteins in rice grains, was constant during both periods.     

Effects of copper on the decomposition of plant residues,microbial biomass,and β-glucosidase activity in soils

Abstract

Two types of soils (Brown Lowland soil and Ando soil), which were artificially enriched with different amounts of Cu, were incubated with or without pulverized orchard grass for 12 weeks at 25°C. For both soils with and without orchard grass amendment, the amount of CO₂ evolved over the 12-week period of incubation decreased by the enrichment with Cu at a concentration exceeding 1,000 mg kg^?1 soil. The decrease of the mineralization of added orchard grass in the Cu-enriched soil was conspicuous especially during the initial period of incubation. The amount of microbial biomass C at the end of the incubation was significantly reduced by the Cu enrichment regardless of the amendment with orchard grass. The relative decrease of the soil microbial biomass was much greater than that of the soil respiration. The amount of biomass C was negatively correlated with the amount of 0.1 M CaCl₂-extractable Cu as a logarithmic function. On the other hand, the β-glucosidase activity at the end of the incubation was not significantly affected by the presence of Cu in the soils without orchard grass amendment and increased with the increase in the amount of enriched Cu in the orchard grass-amended soils.     

Effects of wood char and activated carbon on the hydrolysis of cellobiose by β-glucosidase from Aspergillus niger

Biochar amendments to soils have been suggested as a strategy to sequester carbon and therefore mitigate global climate change. The enrichment of soils with charred materials also increases their fertility. This fertilising effect of biochar may be caused by various mechanisms; an acceleration of nutrient cycling has been suggested as one such mechanism. The rate-limiting step in nutrient cycling is thought to be the extracellular enzymatic attack on biological macromolecules. In this study, therefore, the effects of chestnut wood char (specific surface area 2.0 m² g⁻¹) and of activated carbon (specific surface area approximately 900 m² g⁻¹) on an extracellular enzymatic reaction involved in the degradation of cellulose (i.e., hydrolysis of cellobiose by β-glucosidase from Aspergillus niger) were investigated. Cellobiose was not adsorbed by chestnut wood char, whereas activated carbon absorbed more than 97% of it. Both charred materials adsorbed more than 99% of β-glucosidase. For chestnut wood char, adsorption of the enzyme caused a decrease of approximately 30% in the reaction rate, whereas for activated carbon, the nearly complete absorption of both substrate and enzyme entirely inhibited the reaction. These results show that β-glucosidase from A. niger retains most of its activity when adsorbed to chestnut wood char and that the reaction it catalyses in nature is only slightly affected by this charred material. On the other hand, a material characterised by a high specific surface area and high porosity, such as activated carbon, can make even a highly soluble substrate unavailable for soil enzymes and therefore completely inhibit the reaction. Thus, charred materials may affect nutrient cycling mainly by regulating the availability of substrates: the degradation of highly soluble substrates may be accelerated by materials with low specific surface area, which maintain an active and protected enzyme pool, whereas materials with high specific surface and high porosity may slow down the degradation by making substrates unavailable.     

Preliminary investigations on the effects of no‐till corn production methods on specific soil mesofauna populations

Abstract

Moldboard plowing, chisel plowing and no‐till methods were used to produce field corn (Zea mays) in an old grass sod. Grain yields were similar ranging between 13,100 and 14,186 kg/ha. The greatest spread in soil temperatures was only 2.1 C with the plowed soil always being warmest. Mean soil organic matter levels of the surface soil ranged between 1.9 percent on the moldboard plowed plots and 3.3 on the no‐till plots. Ten species of soil Collembola were identified. Collembola and Acarina populations were concentrated in the surface 5 cm of soil and were present in greatest numbers in the No‐Till soil.     

Effects of adding low levels of a disulfide reducing agent on the disulfide interactions of β-lactoglobulin and κ-casein in skim milk

Low concentrations of a disulfide reducing agent were added to unheated and heated (80 °C for 30 min) skim milk, with and without added whey protein. The reduction of the β-lactoglobulin and κ-casein disulfide bonds was monitored over time using electrophoresis. The distribution of the proteins between the colloidal and serum phases was also investigated. κ-Casein disulfide bonds were reduced in preference to those of β-lactoglobulin in both unheated and heated skim milk (with or without added whey protein). In addition, in heated skim milk, while the serum κ-casein was reduced more readily than the colloidal κ-casein, the distribution of κ-casein between the two phases was not affected.     

Effects of three biochar types on activity of β-glucosidase enzyme in two agricultural soils of different textures

ABSTRACT

Biochar has attracted significant attention due to the long-lasting nature, and prominent influence on soil characteristics. This study was conducted to evaluate changes in the activity of β-glucosidase enzyme (BG) in loamy and sandy loam texture soils following two winter wheat growing seasons. The experimental design was a randomized complete block with three replicates and four treatments. The treatments were two soils, three biochars (rice husk, corn cob and bean harvest residue), five biochar rates (BR) and five levels of mineral fertilizers (FR) or dairy effluent (DE). The fertilizers were applied at the beginning of each season, while biochars were applied only at the beginning of experiment. Soil samples were collected following the second season and analyzed for the BG activity. Addition of biochars reduced the BG activity and the decline was higher in sandy loam compared to loamy soils. Negative effect of biochar to BG activity was greater at the highest BR (3.0%) than the lower BR. Fertilizer additions along with DE biochar had significant effect on BG activity that increased with higher FR. Given the importance of BG activity in soil organic matter decomposition, biochar application can be considered a sustainable way of increase in carbon sequestration.     

Display of Fibrobacter succinogenes β-glucanase on the cell surface of Lactobacillus reuteri

The aim of this study was to display a rumen bacterial β-glucanase on the cell surface of a probiotic Lactobacillus reuteri strain. The β-glucan degrading ability and the adhesion capability of the genetically modified strain were evaluated. The β-glucanase (Glu) from Fibrobacter succinogenes was fused to the C-terminus of collagen-binding protein (Cnb) from L. reuteri and then expressed by L. reuteri Pg4 as a recombinant Cnb-Glu-His(6) fusion protein. Confocal immunofluorescence microscopy and flow cytometric analysis of the transformed strain L. reuteri pNZ-cnb/glu demonstrated that Cnb-Glu-His(6) fusion protein was displayed on its cell surface. In addition, L. reuteri pNZ-cnb/glu acquired the capacity to break down barley β-glucan and showed higher adhesion capability, in comparison with the parental strain L. reuteri Pg4. To the best of the authors' knowledge, this is the first report of successful display of fibrolytic enzymes on the cell surface of intestinal lactobacilli.     

Application of the “standard cell” concept in research on soil formation

The concept of the “standard cell” of a rock, which compares numbers of cations associated with 160 oxygen ions (Barth, 1948) can be applied to the solid material of soils and saprolites as a means of tracing changes in element content at different depths in soil profiles weathered from igneous rocks. The “standard cells” can be derived readily from total silicate analyses of rock and soil materials and can be expressed as chemical formulae or in tables to facilitate comparisons. Standard cells for several layers from each of three profiles from southeast Queensland illustrate the type of information obtained. Standard cells of solum and weathered rock layers of a profile previously studied by mineralogical techniques permit a comparison of inferences about weathering which have been drawn by the two approaches.     

Comparison of the Effects of Salt Stress and Salt–Alkaline Mixed Stress on the Mineral Nutrition of Sunflower

Sunflowers (Helianthus annuus L.) were treated with salt stress and salt–alkaline mixed stress, which were established by mixing proportions of sodium chloride (NaCl), sodium sulfate (Na₂SO₄), sodium bicarbonate (NaHCO₃), and sodium carbonate (Na₂CO₃). The physiological indices of seedlings, including photosynthesis, growth, and mineral element contents, were determined to compare differences in the physiological responses of sunflower to salt stress and salt–alkaline mixed stress. The results showed that the destructive effects of salt–alkaline mixed stress on growth and photosynthesis were more severe than those of salt stress. The contents of mineral elements in the plants stressed were significantly less under salt–alkaline mixed stress than under salt stress. Data analysis indicated that both stresses caused ionic activity and free concentrations of some mineral elements to decline and even caused precipitation. This was coupled with a reduction of absorption capacity of roots. In conclusion, the mineral nutrition in sunflowers was affected under both salt and salt–alkaline mixed stresses, but the effects were more severe under salt–alkaline mixed stress than salt stress.     

Effects of river infrastructures on the floodplain sedimentary environment in the Rhône River

Journal of Soils and Sediments - River infrastructures such as dikes, groynes, and dams are ubiquitous on most large rivers, and although their consequences on the riverbed morphology have often...     

Sulphur nutrition of clover: Effects of plant age on the composition‐yield relationship

Abstract

Five levels of S (as gypsum) were topdressed on to a pasture containing subterranean clover. Yield responses by the clover fraction of the pasture were measured, together with the total S concentration, the sulphate S concentration and the N:S ratio in young clover plants collected in autumn prior to the fertilizer application, and in plants from the control plots collected in winter and in spring.

As there were irregular changes in analytical values from autumn through winter to spring, a more detailed series of samples was taken in the second year. Therein, values for total S and sulphate S in clover plants from each treatment rose during the winter and fell during the spring, so that the critical concentration of sulphur or sulphate varied during the growing period. However, the critical N:S ratio was relatively stable, and in view of previously demonstrated correlations with response to added S, appears to be the more useful index for both diagnosis and prognosis.     

The formation of spherical clay balls on the slopes of sandpit quarry,the Rudice-Seč (Czech Republic)

Clays on the steep slopes of a small artificial sedimentary basin (sandpit quarry) alternately desiccate/crack and erode during hot dry and rainy seasons, respectively. Small irregular blocks of clay (IBC) predefined by cracks are released by gully erosion. The IBCs are quickly transported to the slope base and then rolled down the basin bottom by runoff. Due to rotation about a randomly changing axis and under an effect of deformation forces, the IBCs are transformed into spherical clay balls (SCB). The SCBs are transported, in general, towards the depositional base where they are buried by assorted sandy/clayey sediment. As soon as the kinetic energy of the runoff has decreased, the SCBs remain spread along transport path. From their distribution, the shaping process was evaluated. Based on data processing, the dependency,relating the clay ball projection sphericity, ψ, to the effective diameter, d_ef, and to the distance from the steep slope base, λ, was found. Based on this equation, the length of clay ball transport can be estimated if the projection sphericity and effective diameter are known.     

Effect of the substrate concentration and water activity on the yield and rate of the transfer reaction of β-galactosidase from Bacillus circulans

Prebiotic galactosyl oligosaccharides (GOS) are produced from lactose by the enzyme β-galactosidase. It is widely reported that the highest GOS levels are achieved when the initial lactose concentration is as high as possible; however, little evidence has been presented to explain this phenomenon. Using a system composed of the commercial β-galactosidase derived from Bacillus circulans known as Biolacta FN5, lactose and sucrose, the relative contribution of water activity, and substrate availability were assessed. Oligosaccharide levels did not appear to be affected by changes in water activity between 1.0 and 0.77 at a constant lactose concentration. The maximum oligosaccharide concentration increased at higher initial concentrations of lactose and sucrose, while initial reaction rates for transfer increased but remained constant for hydrolysis. This suggests that the high oligosaccharide levels achieved at the raised initial saccharide concentration are due to increases in reactions that form oligosaccharides rather than decreases in concurrent reactions, which degrade oligosaccharides. There were different effects from changing the initial concentration of lactose compared to sucrose, suggesting that the ability of lactose to act as a donor saccharide may be more important for increasing maximum oligosaccharide concentrations than the combined ability of both saccharides to act as galactosyl acceptors.     

Application of Ground Phosphate Rock to Dimmish the Effects of Simulated Acid Rain on Soil Properties

The effects of simulated acid rain retained in soil on the properties of acid soil and its diminishing by application of ground phosphate rock were investigated by using the sorption method.Results show as follows:(1)For yellow brown soil,the effect of simulated acid rain on the properties of soil with a pH value of 5.9 was relatively small,except a great quantity of acid rain deposited on it.(2) for red soil,the effect of simulated acid rain on the properties of soil was significant.With the increase of the amount of acid deposition,the pH value of soil was declined,but the contents of exchangeable H^ ,Al^3 and Mn^2 and the amount of SO4^1- retention were increased.(3) Many properties of acid soils could be improved by applying ground phosphate rock.For example,pH value of soils and the amounts of available P and exchangeable Ca^2 and Mg^2 were increased,and the amounts of exchangeable H^ and Al^3 and SO4^2- retained was reduced.The application of ground posphate rock could effctively diminish the pollution of acid rain to soil.     

Effects of environmental stress on the relationship of free amino‐N to fresh weight of rice plants

A study was made on the effects of light and temperature combinations, nitrogen (N) carriers, and N levels on the growth and free amino‐N pool size of rice plants. The experiment was conducted in a growth chamber in nutrient solution. Combinations of high light supply (40,000 lux), or high temperature (35°C), or both, resulted in accumulation of large amino‐N pools.

There were negative relationships between free amino‐N pool sizes and the fresh weight of plants. These negative relationships could be best described by decay curves. On the other hand, in plants under the low light (17,280 lux) and low temperature (24°C) combination, the amino‐N to fresh weight relationship was positive, and fitted to a straight line.

The amino‐N to free sugars relationship fitted decay curves, irrespective of light and temperature combinations. The ammonium‐N to amino‐N relationship under high light and/or high temperature combinations showed a quadratic response. Analysis of these curves suggests that deamination takes place as free NH₄‐N increases in plants. The results show a strong effect of the free sugar pools on the N‐metabolism of plants under stress. We suggest the decay type curves are a characteristic of the amino‐N versus fresh weight relationships of plants under environmental stress.     

Effects of the 1982–1983 El Nino on Humboldt penguin colonies in Peru

The Humboldt penguin Spheniscus humboldti is endemic to the Peruvian Current which flows northward along the coast of Chile and Peru. This species has greatly diminished from its former abundance.The coast of Peru is characterised by high biological productivity which concentrates fish such as the anchovy Engraulis ringens, the main prey item of marine predators including seabirds. In years of the abnormal oceanographic conditions of El Nino, the schools of anchovies become unavailable to the seabirds and they disperse in search of food. Massive mortality, especially of juveniles, results and there is nest desertation and lack of reproduction.This paper describes the effects of the 1982–1983 El Nino on Humboldt penguin colonies in Peru. There has been an overall population decline of 65% and the surviving population in 1984 was estimated to be between 2100 and 3000 adults. Although El Nino is a periodic event and the Humboldt penguin has evolved to adapt to such unpredictable changes, the environment has now been altered by man. Under these circumstances, the 1982–1983 El Nino has contributed to placing this species in a critical position.     

Landscape and Land‐Use Effects on the Spatial Variation of Soil Chemical Properties

The current study addressed the spatial variation of soil organic matter (SOM), total nitrogen (TN), extractable phosphorus (EP), and extractable potassium (EK) in agricultural soils of a representative region, northeast China. Soil cation exchange capacity (CEC) and the effects of landscape attributes and land use were also investigated. The techniques used included conventional statistics, geostatistics, and geographic information systems (GIS). Our study demonstrated that EP had the greatest coefficient of variation (CV), and CEC had the least CV. The experimental semivariograms of the five soil chemical properties included in this study were all fitted with exponential models. The five soil variables all showed moderate spatial dependence. The SOM, EK, and CEC decreased with increasing altitude. Significant negative relationships were found between the slope gradient and EP, EK, and CEC. Relatively steeper slopes might result in greater soil erosion, which leads to a decline in soil nutrients. Soil types had significant impacts on all soil chemical properties, which reflect the effect of the parent soil material. In general, the mean values of soil variables for vegetable land were statistically greater than those for upland and paddy fields. After being divided into two parts along the Yinma River, soil samples of the western part have statistically greater SOM, EP, EK, and CEC values than those collected from the eastern part.