Biochemical and molecular control of cold-induced sweetening in potatoes

The benefits of being able to process potatoes directly into chips or fries from cold storage (2 to 4 C) include less shrinkage, retention of dry matter, decreased disease loss, extended marketability, and the elimination of the need for dormancy-prolonging chemicals. Unfortunately at low temperature, potato tubers undergo a phenomenon known as cold-induced sweetening where the rate of conversion of starch to reducing sugars (i.e., glucose and fructose) is accelerated. As raw potatoes are sliced and cooked in oil at high temperature, the accumulated reducing sugars react with free amino acids in the potato cell forming unacceptably brown- to black-pigmented chips or fries via a non-enzymatic, Maillard-type reaction. Potatoes yielding these unacceptably colored products are generally rejected for purchase by the processing plant. All commercial potato cultivars presently used for the production of potato chips and fries accumulate excess free reducing sugars when exposed to cold stress. If a “cold-processing potato” was available, energy savings would be realized in potato-growing regions where outside storage temperatures are cool. In regions where outside temperatures are moderately high, increased refrigeration costs may occur. This expense would be offset, however, by removal of the need to purchase dormancy-prolonging chemicals, by a decreased need for disease control and by improvement of long-term tuber quality. The primary goal of this review is to describe recent research of a biochemical and molecular nature that relates to the underlaying mechanisms regulating post harvest, cold-induced sweetening in potato tubers. No attempt was made to outline the extensive research conducted on the genetic manipulation of carbon metabolism between starch and free sugars during photosynthesis and/or during potato development in relation to source/sink interactions.     

Climate Change and Consequences for Potato Production: a Review of Tolerance to Emerging Abiotic Stress

Potato is a major global crop that has an important role to play in food security, reducing poverty and improving human nutrition. Enhanced atmospheric CO₂ concentrations provide an opportunity to increase potato yields in the future, but this will only be possible if the potato crop can cope with the other consequences of climate change caused by this rise in CO₂. While climate change may impact biotic stress either positively or negatively, abiotic stresses are likely to be greatly increased and become a major threat to potato production. Increasing heat, drought and salinity stress will drive the need for greater understanding of genes, traits and management techniques that allow potato to cope with these stresses. In this review, we identify some of the key physiological and molecular adaptations of potato to these stresses and propose an ideotype which should include (1) optimal stomatal regulation to balance water loss and heat stress in leaves, (2) production of metabolites and transporters to scavenge reactive oxygen species and partition toxic elements, (3) enhanced root systems to maximise water capture, (4) maintenance of tuberisation under stress conditions and (5) stress avoidance by accelerating crop development and reducing time to yield. We discuss potential ways to achieve this ideotype, emphasising the need to benefit from genetic diversity in landrace and wild material by screening for traits in combined stress environments appropriate to future agroecosystems.     

Furrow vs hill planting of sprinkler-irrigated russet burbank potatoes on coarse-textured soils

Surface water runoff from the hill, where potatoes are planted, to the furrow may exacerbate potato drought sensitivity. Planting into furrows and constructing midrow ridges may improve water use efficiency and relieve water stress on potato by directing water toward, not away from, the plants. A 3-year field study was conducted to compare yields and tuber size distributions of furrow- and hill-planted potato (Solanum tuberosum L., ‘Russet Burbank’) on coarse-textured, well-drained soils under sprinkler irrigation. A split-plot experimental design with main plots of row orientation (N-S vs E-W) and subplots of planting method (hill and furrow) combined with two planting depths was used at two central North Dakota sites. Except for planting method and limiting the post-emergence cultivation in the furrow treatments, all cultural practices (fertilizer, irrigation, etc.) were identical and corresponded with conventional practices for hill planted potato. Row orientation did not affect yield for any tuber size category. Averaged over 3 years, furrow-planted potato produced 24% larger tubers (188 vs 151 g), 31% smaller yield for tubers <113 g (4.99 vs 7.21 Mg ha^?1), 28% smaller yield for tubers 113 to 170 g (8.14 vs 11.3 Mg ha^?1), 8% larger yields for tubers 170 to 283 g (18.0 vs 16.6 Mg ha^?1), 103% larger yields for tubers 283 to 454 g (10.9 vs 5.36 Mg ha^?1), 341% larger yields for tubers >454 g (2.65 vs 0.60 Mg ha^?1), and 10% larger total yields (46.2 vs 41.9 Mg ha^?1) compared with hill-planted potato. There were no differences in tuber specific gravity. Preliminary soil water measurements indicated an inter-row water-harvesting effect for furrow planting compared with hill planting. The furrow-planting method may offer significant potential for ameliorating the drought sensitivity of potato.     

Drip as Alternative Irrigation Method for Potato in Florida Sandy Soils

Seepage irrigation is the most common irrigation system for potato production in Florida, which relies on control of the water table to irrigate the crop. A 2-year trial was established to evaluate the feasibility of drip irrigation as an alternative to seepage for potato production. The performance of ‘Atlantic’, ‘Fabula’, and ‘Red LaSoda’ varieties were evaluated by comparing two drip tape installation depths, surface (SUR) and subsurface (SUB) with seepage (SEP). The overall potato total yield was 25.3; 19.2 and 29.9 Mg ha^?1 for SUR, SUB and SEP, respectively. The SUR and SEP treatments yielded similarly for ‘Fabula’ in both years and ‘Atlantic’ in 2011. The ‘Red LaSoda’ consistently obtained lower yields under both drip treatments compared to SEP. Conversely, drip irrigation promoted significant reduction of the incidence of tuber physiological disorders such as brown center, hollow heart, and internal heat necrosis.     

Projecting Future Crop Evapotranspiration and Irrigation Requirement of Potato in Lower Gangetic Plains of India using the CROPWAT 8.0 Model

A study on evapotranspiration from potato fields was conducted in the Lower Gangetic Plains of India. The input data required for the CROPWAT irrigation management model was collected, and evapotranspiration (ET) and irrigation water requirement (IWR) for potato crops were calculated using the model. Firstly, the CROPWAT model was validated by comparing simulated crop evapotranspiration (SET) with actual ET calculated through the field water balance method. Thereafter, SET and IWR for nine locations in the lower Gangetic plains of India were calculated for the period from 1996–1997 to 2008–2009, for the current situation (using 20-year-average weather data of the stations), and for elevated thermal conditions, i.e. considering 2 and 3 °C increases over the current temperature. The future change in IWR for potato up to 2050 was also calculated considering the projected climatic scenario generated by the PRECIS model. The CROPWAT calculated IWR values showed an increasing, though not statistically significant, trend in requirement of irrigation water for potato across the nine locations during the period from 1996–1997 to 2008–2009. At a temperature increase of 2 °C over normal, the mean SET of potato would increase by 0.06 mm per day and the average IWR would be 6.0 mm per season more. If the mean temperature would be 3 °C more than normal, the SET would be 0.16 mm day^?1 higher and the IWR 16.6 mm. Also based on the projected climatic scenario generated by the PRECIS model, the future SET up to 2050 showed an increasing trend. The present study indicates increasing demand for irrigation water, which may significantly affect the agricultural scenario in the region.     

Heat stress and the tuberization stimulus

Heat stress may lower potato tuber yields through reduction in the net amount of photosynthate available for total plant growth and through reduced partitioning to the tubers. The latter effect is generally more serious and is the main subject of this paper. High temperatures have much the same effect on partitioning as do long days, and there are significant genetic differences in response to both factors. Both affect the total morphology of the plant. A cutting technique may be used to rapidly screen seedling populations for reaction to temperature or photoperiod. Although progress has been made in studying the nature of the hypothetical “tuberization stimulus”, more needs to be done to clarify the physiological and biochemical changes involved during tuber induction.     

Potato and Human Exploration of Space: Some Observations from NASA-Sponsored Controlled Environment Studies

Future space exploration by humans will require reliable supplies of food, oxygen and clean water to sustain the expeditions. Potato is one of several crops being studied for such a “life support” role. Tests sponsored by the US National Aeronautics and Space Administration (NASA) confirmed the well-known short day tendencies for tuberisation, but also revealed that some cvs. (e.g., Norland, Denali and Russet Burbank) could tuberise well under continuous high light. Horticultural tests showed that plants grew well and tuberised readily using a nutrient film technique (NFT). CO₂ enrichment studies with potato showed typical C₃ responses in photosynthesis and yield, with maximum rates occurring near 1000 μmol mol⁻¹. The highest tuber yields from these controlled environment studies reached 19.7 kg FM m⁻² or equivalent to nearly 200 t ha⁻¹. This equated to a productivity of 38 g m⁻² DM m⁻² day⁻¹. Stand evapotranspiration (ET) rates ranged from 3.4 to 5.2 l m⁻² day⁻¹ throughout growth, while maximum ET rates for canopies could approach 10 l m⁻² day⁻¹. Harvest indices (tuber DM/total DM) typically ranged from 0.7 to 0.8, indicating that waste (inedible) biomass from potato would be less than that from many other crops. An experiment was conducted in 1995 on NASA’s Space Shuttle using excised potato leaves to study tuber formation at axillary buds during spaceflight. The results showed that tubers formed equally well in space as in the ground controls, indicating that reduced gravity should not be an impediment to tuberisation.     

Evaluation of Potato Cultivars for Resistance Against Water Deficit Stress Under In Vivo Conditions

Water deficit is the most important abiotic stress factor in crop production. Evaluation of the response of different potato cultivars to water deficit stress is necessary to release cultivars for regions with water deficit. A split-plot experiment with three replications was carried out during 2005 and 2006. The main factor consisted of three levels of irrigation (irrigation after 25%, 35% and 50% discharge of the available water, i.e. normal conditions, mild stress and severe stress, respectively), and the split factor included seven potato cultivars (Agria, Savalan, Satina, Caesar, Kennebec, Marfona and Santé). Cultivars Savalan, Caesar and Kennebec had higher total and marketable tuber yield, water use efficiency, and values for stress tolerance indices than the other cultivars, both under mild and severe stress conditions. Cultivars Caesar and Kennebec were selected as tolerant cultivars; cultivars Savalan and Satina were identified as moderately tolerant cultivars whereas cultivars Agria, Marfona and Santé proved to be susceptible to water deficit.     

Effects of Exogenous Chitosan on Physiological Characteristics of Potato Seedlings Under Drought Stress and Rehydration

Using young plants of the potato variety “Favourite”, we studied the effects of exogenous chitosan (CTS) on physiological characteristics of potatoes under drought stress and rehydration. Spraying 50, 100 and 200?mg l^?1 of exogenous CTS on potato leaves before drought stress reduced membrane relative permeability and malondialdehyde concentration of potato leaves, raised the concentration of proline and soluble proteins and enhanced the activities of superoxide dismutase and peroxidase during drought stress. Additionally, CTS promoted the recovery of these physiological indicators after a rehydration period. Of the three treatments, 100?mg l^?1 of CTS alleviated drought stress the best. Altogether, this indicates that exogenous CTS could relieve drought stress damage in young potato plants by enhancing their antioxidation ability, increasing the activities of protective enzymes and regulating the content of osmotic regulatory substances. Applying exogenous CTS could be an effective measure to reduce drought stress in potato and warrants further evaluation in the field.     

Climate Change and Potato Production in Contrasting South African Agro-Ecosystems 2. Assessing Risks and Opportunities of Adaptation Strategies

This study aims to assess the risks and opportunities posed by climate change to potato growers in South Africa and to evaluate adaptation measures in the form of changes in planting time growers could adopt to optimise land and water use efficiencies in potato, using a climate model of past, present-day and future climate over southern Africa and the LINTUL crop growth model. This was done for distinct agro-ecosystems in South Africa: the southern Mediterranean area where potato still is grown year round with a doubling of the number of hot days between 1960 and 2050, the Eastern Free State with summer crops only and Limpopo with currently autumn, winter and spring crops where the number of hot days increases sevenfold and in future the crop will mainly be grown in winter. A benefit here will be a drastic reduction of frost days from 0.9 days per winter to 0. Potato crops in the agro-ecosystems will benefit considerably from increased CO₂ levels such as increased tuber yield and reduced water use by the crop, if planting is shifted to appropriate times of the year. When the crop is grown in hot periods, however, these benefits are counteracted by an increased incidence of heat stress and increased evapotranspiration, leading in some instances to considerably lower yields and water use efficiencies. Therefore year-round total production at the Sandveld stabilizes at around 140 Mg?ha^?1 (yield reduction in summer and yield increase in winter), increases by about 30% in the Free State and stays at about 95 t?ha^?1 at Limpopo where yield increase due to CO₂ is annulled by a shorter growing season. When the crop is grown in a cool period, there is an additional benefit of a reduced incidence of cold stress and a more rapid canopy development in the early stages of crop growth. In all three areas, potato growers are likely to respond to climate change by advancing planting. In Limpopo, a major benefit of climate change is a reduction in the risk of frost damage in winter. The relevance of these findings for potato grown in agro-ecosystems elsewhere in the world is discussed.     

Commodity program flexibility and U.S. potato acreage

A new proposal for U.S. farm commodity programs would allow more flexibility in planting decisions on program acreage. This work examines the effect of such a proposal on potato production acreage. These results are used to project changes in total revenue accruing to existing potato producers and to the potato industry as a whole. The results indicate that the short term impact on the potato industry as a whole would be relatively minor but the impact could be unevenly distributed across producing regions of the U.S., causing some potential local discomfort.     

草坪草对干旱胁迫的反应概述

干旱胁迫是影响草坪草生长最主要的环境胁迫因子之一。在干旱胁迫下,草坪草的根、茎、叶均会表现出一定的生态适应性,还会发生叶片蒸腾和光合作用减弱、渗透调节物质累积、活性氧代谢失调等生理生化反应,而且在细胞水平和基因水平上也会发生变化,这都与草坪草的抗旱调节机制有着密切的联系。目前我国关于草坪草的抗旱性研究还处于起步阶段,基因工程技术的研究和应用是今后的主要发展方向。     

大豆耐低磷研究进展

大豆是世界重要的粮油兼用作物,也是人类优质蛋白及畜牧业饲料蛋白的主要来源。大豆是喜磷作物,磷不仅是大豆遗传物质的重要组成成分,同时参与大豆体内酶促、新陈代谢、根瘤固氮等生理生化过程。全世界耕地中近1/2的耕地处于缺磷状态,我国耕地中大约有2/3的耕地属于缺磷状态,磷胁迫是限制大豆产量最主要的因素之一。文章分别从低磷胁迫下大豆形态学变化以及大豆生理生化反应、遗传图谱的构建及大豆耐低磷QTL定位研究进展进行了初步概述,并对大豆耐低磷QTL定位进行了展望,以期为我国大豆耐低磷遗传育种研究提供参考。     

Early blight in South Africa: Knowledge, attitudes and control practices of potato growers

Summary A survey of control practices and grower perceptions of early blight in South Africa was conducted from May 2001 — July 2002 using an informally structured questionnaire. Questionnaires were collected from the 10 largest of the 14 potato production regions in South Africa. The predominant control method for early blight was the use of fungicides with mancozeb or chlorothalonil as active ingredient. Most respondents commence spraying at flowering and spray at 7–10 day intervals thereafter. BP1, Up-to-date and Mondial were the most commonly planted cultivars. The majority of farmers indicated that they would be willing to use an early blight decision support system if an accurate and cost-effective one was available, and that they believe more research is necessary on early blight in South Africa. Estimated economic losses ranged from 1–60%, with the average being approximately 20%. This is the first such survey to be conducted in South Africa.     

Climate Change and Potato Production in Contrasting South African Agro-ecosystems 1. Effects on Land and Water Use Efficiencies

Explorations of the impact of climate change on potential potato yields were obtained by downscaling the projections of six different coupled climate models to high spatial resolution over southern Africa. The simulations of daily maximum and minimum temperatures, precipitation, wind speed, and solar radiation were used as input to run the crop growth model LINTUL-Potato. Pixels representative for potato growing areas were selected for four globally occurring agro-ecosystems: rainy and dry winter and summer crops. The simulated inter-annual variability is much greater for rainfall than for temperature. Reference evapotranspiration and radiation are projected to hardly decline over the 90-year period, whilst temperatures are projected to rise significantly by about 1.9 °C. From literature, it was found that radiation use efficiency of potato increased with elevated CO₂ concentrations by almost 0.002 g?MJ^?1?ppm^?1. This ratio was used to calculate the CO₂ effect on yields between 1960 and 2050, when CO₂ concentration increases from 315 to 550 ppm. Within this range, evapotranspiration by the potato crop was reduced by about 13% according to literature. Simulated yield increase was strongest in the Mediterranean-type winter crop (+37%) and least under Mediterranean summer (+12%) and relatively warm winter conditions (+14%) closer to the equator. Water use efficiency also increased most in the cool rainy Mediterranean winter (+45%) and least so in the winter crop closer to the equator (+14%). It is concluded from the simulations that for all four agro-ecosystems possible negative effects of rising temperatures and reduced availability of water for potato are more than compensated for by the positive effect of increased CO₂ levels on water use efficiency and crop productivity.     

Physiological mechanisms associated with the development of internal necrotic disorders of potato

Physiological disorders such as Brown Center and Internal Brown Spot affect the quality of potato tubers destined for fresh and processing markets. Cultural and environmental factors giving rise to such disorders have been studied extensively. Considerably less information is available on the underpinning physiological, molecular and biochemical mechanisms. However, the cell’s calcium status clearly plays an important role in resistance responses. Whilst extreme genotypic susceptibility to these disorders exist such variation has not been exploited fully to dissect the genetics and biochemistry of the key processes. This is central to the selection of resistant germplasm or to the manipulation of existing genotypes for improved tolerance     

Growing maize in clumps as a strategy for marginal climatic conditions

Under dryland conditions of the Texas High Plains, maize (Zea mays) production is limited by sparse and erratic precipitation that results in severe water stress particularly during grain formation. When plant populations are reduced to 2.0–3.0 plants m⁻² to conserve soil water for use during grain filling, tillers often form during the vegetative growth and negate the expected economic benefit. We hypothesized that growing maize in clumps spaced 1.0 m apart would reduce tiller formation, increase mutual shading among the plants, and conserve soil water for grain filling that would result in higher grain yield. Studies were conducted during 2006 and 2007 at Bushland, TX. with two planting geometries (clump vs. equidistant), two irrigation methods (low-energy precision applicator, LEPA, and low-elevation spray applicator, LESA) at three irrigation levels (dryland, 75 mm and 125 mm in 2006; and dryland, 50 mm and 100 mm in 2007). For dryland plots in 2007, clump plants had only 0.17 tillers (0.66 tillers m⁻²) compared with 1.56 tillers per plant (6.08 tillers m⁻²) for equidistant spacing. Tillers accounted for 10% of the stover for the equidistant plants, but less than 3% of the grain. Clump planting produced significantly greater grain yields (321 g m⁻² vs. 225 g m⁻² and 454 g m⁻² vs. 292 g m⁻² during 2006 and 2007, respectively) and Harvest Indexes (0.54 vs. 0.49 and 0.52 vs. 0.39 during 2006 and 2007, respectively) compared with equidistant plants in dryland conditions. Water use efficiency (WUE) measurements in 2007 indicated that clumps had a lower evapotranspiration (ET) threshold for initiating grain production, but the production function slopes were 2.5 kg m⁻³ for equidistant treatments compared to 2.0 kg m⁻³ for clump treatments. There was no yield difference for method of irrigation on water use efficiency. Our results suggest that growing maize in clumps compared with equidistant spacing reduced the number of tillers, early vegetative growth, and Leaf Area Index (LAI) so that more soil water was available during the grain filling stage. This may be a useful strategy for growing maize with low plant populations in dryland areas where severe water stress is common.     

Benchmarking sunflower water productivity in semiarid environments

Appropriate benchmarking is essential for evaluating the efficiency with which crops use water and for identifying constraints, other than water, to crop yield. No benchmark exists for sunflower. Boundary and simulation analyses were used to quantify the water productivity of sunflower crops grown in the Western Pampas (semiarid central Argentina). The approach involved the use of a large database (n = 169) collected in farmers’ fields over a period of 4 years, and the application of a crop simulation model in combination with actual weather and soil data. Using field data, an upper bound of 8 kg grain ha⁻¹ mm⁻¹ for water productivity, with an apparent seasonal soil evaporation of 75 mm, was defined. Seasonal water supply exceeded maximum expected seasonal crop requirements (ca. 630 mm) for many crops, and a majority of crops with <630 mm of available water during the season had water productivities considerably lower than the upper bound. The field data-based upper bound was indistinguishable from that obtained using yields for a set of 47 simulations using observed initial values for soil water and nitrogen profiles. Simulation confirmed the main features of the boundary-analysis applied to field data, and many simulated crops had yields that fell below the boundary function, even when simulated yield was plotted against simulated seasonal evapotranspiration or transpiration. Long-term (33-year) simulation analyses for two sites showed that most sunflower crops in the area are subjected to episodes of transient and unavoidable water stress after floral initiation. High levels of available soil water at sowing moderate, but in most years do not eliminate, these exposures to water stress. Yield gaps with respect to the boundary function were associated with deficient or excessive rainfall during grain filling, and other, non-water related, factors such as inadequate crop nutrition, biotic stresses, low photothermal quotients during the interval close to anthesis, and lodging. A grain yield/seasonal evapotranspiration plot for a large (n = 154) data set from experiments conducted by others in five separate environments suggests that the boundary function found for the Western Pampas is broadly applicable. Sunflower water productivity, corrected for oil-synthesis costs and seasonal vapour pressure deficit differences, approximates that of winter cereals grown in Mediterranean environments.