Occurrence of Azoxystrobin, Propiconazole, and Selected Other Fungicides in US Streams, 2005�C2006

Fungicides are used to prevent foliar diseases on a wide range of vegetable, field, fruit, and ornamental crops. They are generally more effective as protective rather than curative treatments, and hence tend to be applied before infections take place. Less than 1% of US soybeans were treated with a fungicide in 2002 but by 2006, 4% were treated. Like other pesticides, fungicides can move-off of fields after application and subsequently contaminate surface water, groundwater, and associated sediments. Due to the constant pressure from fungal diseases such as the recent Asian soybean rust outbreak, and the always-present desire to increase crop yields, there is the potential for a significant increase in the amount of fungicides used on US farms. Increased fungicide use could lead to increased environmental concentrations of these compounds. This study documents the occurrence of fungicides in select US streams soon after the first documentation of soybean rust in the US and prior to the corresponding increase in fungicide use to treat this problem. Water samples were collected from 29 streams in 13 states in 2005 and/or 2006, and analyzed for 12 target fungicides. Nine of the 12 fungicides were detected in at least one stream sample and at least one fungicide was detected in 20 of 29 streams. At least one fungicide was detected in 56% of the 103 samples, as many as five fungicides were detected in an individual sample, and mixtures of fungicides were common. Azoxystrobin was detected most frequently (45% of 103 samples) followed by metalaxyl (27%), propiconazole (17%), myclobutanil (9%), and tebuconazole (6%). Fungicide detections ranged from 0.002 to 1.15 ??g/L. There was indication of a seasonal pattern to fungicide occurrence, with detections more common and concentrations higher in late summer and early fall than in spring. At a few sites, fungicides were detected in all samples collected suggesting the potential for season-long occurrence in some streams. Fungicide occurrence appears to be related to fungicide use in the associated drainage basins; however, current use information is generally lacking and more detailed occurrence data are needed to accurately quantify such a relation. Maximum concentrations of fungicides were typically one or more orders of magnitude less than current toxicity estimates for freshwater aquatic organisms or humans; however, gaps in current toxicological understandings of the effects of fungicides in the environment limit these interpretations.     

Nitrogen and fungicide effects on winter wheat produced in the Louisiana Gulf Coast region

Management decisions affecting wheat (Triticum aestivum L.) yield include nitrogen (N) fertility rate, cultivar selection, and fungicide application. Since these cultural practices may interact with each other and the environment, often times affecting grain yield, test weight, and disease development, they need to be better defined for wheat production in for the Gulf Coast region. Field experiments were conducted to evaluate the effect of N rate, fungicide, and cultivar on grain yield, test weight, and disease development. Three N rates (67, 101, and 134 kg#lbha^‐1), two fungicide treatments (no‐fungicide control and fungicide), and four high‐yielding wheat cultivars with different levels of resistance to leaf rust (Puccinia recondite Rob. ex f. sp. tritici) were evaluated in 12 Louisiana field environments. Environment and interaction between environment and N rate, fungicide, and cultivar were significant for grain yield, leaf rust ratings, and test weight. Grain yield response to N rate was linear in five environments and quadratic in five environments. Although there was little evidence of a N x fungicide interaction for grain yield, increasing N rates increased leaf rust severity. Grain yields were increased by fungicide application in only four of twelve environments with a significant cultivar x fungicide interaction for grain yield. Grain yield increase from foliar fungicides was related to leaf rust severity and resistance of cultivars to leaf rust. Test weight was increased due to N in three environments and decreased in two environments. Test weight increased with fungicide application and varied among cultivars. The findings indicate that 101 kg N ha is probably adequate for maximum yield in most years. Although the higher N rates may increase yield in some years, disease pressure and the need for fungicides may also be increased.     

密云水库流域地下水硝态氮的分布及其影响因素

2008年11月至12月,采集了密云水库流域305个井的地下水样品,分析了其硝态氮含量。结果表明,密云水库流域地下水的硝态氮含量的平均值、超标率(10 mg L-1≤NO3--N<20 mg L-1)和严重超标率(NO3--N≥20 mg L-1)分别为6.81 mg L-1、13.77%和2.30%。其中村庄和菜地的地下水硝酸盐污染最为严重,35个村庄井和13个菜地井的地下水硝态氮含量的平均值分别为9.52 mg L-1和9.55 mg L-1,已接近WHO饮用水硝态氮含量10 mg L-1的限定标准,超标率分别为20%和15.38%,严重超标率分别为8.57%和7.69%。219个粮田井水的硝态氮水平位居中间,其硝态氮含量的平均值、超标率和严重超标率分别为6.59mg L-1、14.61%和1.37%。10个林地井的地下水硝态氮含量是最低的,其平均值为2.66 mg L-1,无超标现象。潮河流域农田地下水的硝酸盐污染比白河流域严重。潮河流域农田(124个井)的地下水硝态氮含量的平均值、超标率和严重超标率分别为8.42 mg L-1、21.77%和3.23%,而白河流域(122个井)则分别为5.03mg L-1、6.56%和0,即无严重超标现象。密云水库流域农田地下水的硝态氮含量呈现出上游低而下游高的趋势。玉米田地下水硝态氮含量在接近河道的地方有所降低,与地下水水位呈负相关,与化肥氮的施用量呈正相关,当地下水位小于7m时或当一年的化肥氮的施用量超过200 kg hm-2,存在地下水硝态氮含量超标的潜在危险。     

干旱区重度和轻度盐碱地包气带水分运移规律

为揭示干旱区不同灌溉模式下的包气带水分运移规律,该研究综合使用原位观测、同位素示踪和数值模拟等方法,对跨流域调水背景下,克拉玛依农业开发区重度和轻度盐碱地棉田的土壤水势、土壤含水率和土壤水同位素组成特征,包气带水量平衡以及水分运移规律进行了研究。研究表明,土壤基质势调控的滴灌模式下,重度和轻度盐碱地的灌溉入渗主要影响深度是地表以下0~150 cm,土壤含水率和土壤水势对灌溉和蒸散发动态变化的响应明显,具有前期土壤水和观测期内灌溉入渗水的混合特征。深层(重度盐碱地150~260 cm;轻度盐碱地250~350 cm)受地下水毛细作用影响,土壤水势和土壤含水率对地下水埋深动态变化的响应明显,具有前期土壤水与地下水的混合特征。轻度盐碱地中间层(150~250 cm),几乎不受灌溉入渗和地下水毛细作用的影响,土壤水势和土壤含水率处于动态平衡,主要为前期土壤水的特征。HYDRUS-1D数值模拟结果表明,深层土壤水与地下水之间存在双向交换,地下水对土壤水以补给作用为主,重度和轻度盐碱地地下水补给占包气带水分来源的比例分别为7.9%和15.0%。该灌溉模式对农业开发区地下水补给有一定的抑制作用,但观测期内区域地下水位抬升幅度在50~60 cm之间,说明存在一定的土壤次生盐渍化和地下水咸化的潜在风险。     

Spatially Explicit Integrated Risk Assessment of Present Soil Concentrations of Cadmium, Lead, Copper and Zinc in The Netherlands

Historic and current agricultural and industrial activities have resulted in accumulation of Cd, Cu, Pb and Zn in soil. To estimate potential risks for ecosystems, agriculture and water quality, an integrated risk assessment was performed for The Netherlands. Risks of metal contamination were assessed on a national scale by comparing present soil concentrations of Cd, Pb, Cu and Zn with critical concentrations of those metals in view of agricultural impacts, ecological impacts and impacts on the quality of groundwater and surface waters. Results show that present soil metal concentrations cause few risks for agriculture or ecosystems; for less than 2% of the surface area present metal levels exceed critical limits. Critical limits for groundwater are only significantly exceeded for Pb (17% of the area), but critical limits for surface water are exceeded throughout the country for Cu and Zn. Taking critical limits used in The Netherlands, the area where exceedances take place is nearly negligible for Cd and low for Pb (less than 3%), but much larger (between 40% and 50%) for both Cu and Zn. Results from this study suggest that accumulation of heavy metals in Dutch soils at present primarily affects the quality of surface waters. This stresses the need for harmonization of soil and water policy. Measures to reduce the load in surface waters to meet target levels, under conditions like those prevailing in The Netherlands, are bound to have an impact on land management.     

Perchlorate accumulation in forage and edible vegetation

The accumulation of perchlorate in vegetation is becoming a concern, with increasing numbers of sites reporting the presence of perchlorate in groundwater and surface water. This study investigated potential perchlorate uptake and distribution by a variety of forage and edible crops in both the laboratory and the field. Perchlorate concentrations in soybean leaves grown in the greenhouse were significantly higher than perchlorate concentrations in soybean seeds and pods. Perchlorate concentrations in alfalfa grown in sand were significantly lower than those in alfalfa grown in soil. The concentration of perchlorate in tomato was lower in the fruit than the leaves. Commercially grown wheat and alfalfa samples all contained perchlorate, 0.72-8.6 mg/kg of fresh weight (FW) in the wheat stems, 0.71-4.4 mg/kg of FW in the wheat heads, and 2.9 mg/kg of FW in alfalfa. All field garden samples tested (including cucumber, cantaloupe, and tomato) that were irrigated with perchlorate-tainted water contained perchlorate at various concentrations ranging from 0.040 to 1.65 mg/kg of FW. Bioconcentration factors (BCF), ratios of plant fresh weight concentrations to estimated or measured groundwater concentrations [(microg/kg of FW)/microg/L], were all in the same order of magnitude ranging from 215 +/- 126 for wheat stems to 233 +/- 264 for wheat heads and to 380 +/- 89 for alfalfa. BCF for garden fruit samples were much lower (0.5-20). Results from this study highlight the potential for perchlorate exposure by routes other than drinking water.     

Microflora of turfgrass treated with fungicides

Fungicides are used widely to manage high-quality turfgrass, but their use could adversely affect the composition of microbial populations. Fourteen fungicides, a nematicide, and five mixed fungicide programs were applied to turfgrass for 3 yr and were tested for their effects on numbers of bacteria, actinomycetes and fungi, and N concentrations. Combinations of fungicides suppressed fungi and stimulated bacteria and actinomycetes more than individual toxicants. Collective microbial groups were generally less affected by each fungicide than were individual species within each group. The rate of decrease of NH⁺₄ concentrations in fertilized turf (presumably via nitrification) was only slightly influenced by fungicides. The more harmful effects of some fungicides to turfgrass may involve induced acidity rather than direct suppression of specific microbial groups.     

15%三唑酮喷施次数对小麦条锈病的防治效果

小麦条锈病是小麦生产上的最主要病害之一,三唑酮在小麦条锈病防治上具有极好的控病增产作用,但随着其长期施用,在天水地区小麦生产上已出现抗药菌株,导致防效偏低。为了给小麦条锈病的科学防治和三唑酮的合理高效使用提供技术支撑,在甘肃省农业科学院植物保护研究所甘谷试验站以感病品种冬小麦新冬43号为指示品种,开展了15%三唑酮可湿性粉剂不同喷施次数对小麦条锈病的田间防效试验。试验结果表明,喷药次数对小麦条锈病的田间防效及指示品种的产量、千粒重、穗粒重有极显著的影响。与不喷药对照相比较,15%三唑酮可湿性粉剂药剂量1 200 g/hm2兑水675 kg喷施1次、喷施2次、喷施3次对小麦条锈病的田间终期防效分别为58.4%、83.6%、98.6%,折合产量分别为5 000.0、5 580.0、6 080.0 kg/hm2,较不喷药对照分别增产58.23%、76.58%、92.40%,千粒重分别提高15.5%、32.4%、38.5%,平均穗粒重分别提高7.6%、10.7%、12.2%。认为选用15%三唑酮可湿性粉剂在小麦条锈病防治达标期(病叶率达到10%)时对小麦进行喷雾防治,间隔7 d喷1次,连喷3次,可达到极好的控病增产效果,值得在小麦生产上推广应用。     

Development of potato late blight (Phytophthora infestans) in differently developed potato crops and the potential to graduate the fungicide dose according to crop biomass

Phytophthora infestans is a serious disease in potato production and control of the disease often requires 10 or more fungicide applications during the growing season. Control is typically carried out using a constant fungicide dose in the entire field although there may be large differences in crop development and biomass across the field. The objective of the study reported in this paper was to test whether the fungicide dose response curve controlling P. infestans was dependent on the crop development and biomass level. If such a biomass-dependent dose response was found it was further the purpose to evaluate the potential to optimize fungicide inputs in potato crops applying a site-specific crop density-dependent fungicide dose. The study was carried out investigating the fungicide dose response controlling P. infestans in potatoes at three biomass densities obtained growing the potato crop at three nitrogen levels. Furthermore, the field experiments included four levels of fungicide dose rate at each nitrogen rate, an untreated control, the recommended fungicide dose rate and two reduced dose rates and the experiments were replicated for three years. Nitrogen level and thus crop density had a limited although significant influence on attack of P. infestans whereas fungicide dose rate by far was the most influential variable. In contrast the interaction of nitrogen rate and fungicide dose rate that would indicate a biomass-dependent dose response was insignificant. If the variation in crop density and biomass level obtained in the study is representative for the variation found cultivating potatoes in heterogeneous fields there seem to be limited potential for fungicide optimization applying fungicides against P. infestans site-specific according to crop density or biomass.     

Dissipation of propiconazole and tebuconazole in peppermint crops (Mentha piperita (Labiatae)) and their residues in distilled oils

The broad-spectrum, systemic fungicides propiconazole (1) and tebuconazole (2) are used to control rust in peppermint (Mentha piperita L.). An analytical method, using gas chromatography combined with detection by high-resolution mass spectrometry, was developed to allow for the simultaneous monitoring of both pesticides in peppermint leaves and oil. Field trials were established to determine the rate of dissipation of tebuconazole and propiconazole in peppermint crops. Three applications of each fungicide were trialed at two rates (125 and 250 g of active ingredient (ai)/ha). At harvest, 64 days after the final application, propiconazole was detected at levels of 0.06 mg/kg and 0.09 mg/kg of dry weight, and tebuconazole was detected at 0.26 and 0.80 mg/kg dry weight, in identical trials. Rates of dissipation of propiconazole and tebuconazole were lower at a second trial site, where three applications of 125 g/ha ai for each fungicide resulted in residue levels of 0.21 mg/kg for both pesticides, detected 89 days after the last application. Propiconazole and tebuconazole were detected in the distilled oil at levels between 0.02 and 0.05 mg/kg and between 0.011 and 0.041 mg/kg, respectively. Propiconazole had a higher tendency to co-distill with the peppermint oil, with 0.7% of that present in the vegetative material ending up in the oil, compared to 0.09% of tebuconazole.     

Quantification of Shallow Groundwater Nutrient Dynamics in Septic Areas

Of all groundwater pollution sources, septic systems are the second largest source of groundwater nitrate contamination in USA. This study investigated shallow groundwater (SGW) nutrient dynamics in septic areas at the northern part of the Lower St. Johns River Basin, Florida, USA. Thirty-five SGW-monitoring wells, located at nine different urban areas served by septic systems, were used to collect the SGW samples seasonally and/or biweekly for a duration of 3?years from 2003 to 2006. Analytical results showed that there were 16 wells with nitrate concentrations exceeding the US Environmental Protection Agency's drinking water limit (10?mg?L^?1). There also were 11 and 14 wells with total Kjeldahl nitrogen (TKN) and total phosphorus (TP) concentrations, respectively, exceeding the ambient water quality criteria (0.9?mg?L^?1 for TKN and 0.04?mg?L^?1 for TP) recommended for rivers and streams in nutrient Ecoregion XII (Southeast USA). In general, site variations are much greater than seasonal variations in SGW nutrient concentrations. A negative correlation existed between nitrate/nitrite?Cnitrogen (NO_x?CN) and TKN as well as between NO_x?CN and ammonium ( $ NH_4^{ + } $ ), whereas a positive correlation occurred between TKN and $ NH_4^{ + } $ . Furthermore, a positive correlation was found between reduction and oxidation (redox) potential and water level, while no correlation was observed between potassium concentration and redox potential. This study demonstrates a need to investigate the potential adverse impacts of SGW nutrients from the septic areas upon the deeper groundwater quality due to the nutrient penetration and upon the surface water quality due to the nutrient discharge.     

Previous Corn Row Effects on Potassium Nutrition and Yield of Subsequent No‐Till Soybeans

Abstract

Narrow‐row soybean [Glycine max (L.) Merr.] production in corn [Zea mays L.]–soybean rotations results in various distances of soybean rows from previous corn rows, yet little is known about soybean responses to proximity to prior corn rows in no‐till systems. The objective of this study was to evaluate the impacts of preceding corn rows on potassium (K) nutrition and yield of subsequent no‐till soybeans. Four field experiments involving a corn–soybean rotation were conducted on long‐term no‐till fields with low to medium K levels from 1998 to 2000 near Paris and Kirkton, Ontario, Canada. In the corn year, treatments included K application rate and placement in conjunction with tillage systems or corn hybrids. Before soybean flowering, soil exchangeable K concentrations (0–20 cm depth) in previous corn rows were significantly higher than those between corn rows. At the initial flowering stage, trifoliate leaf K concentrations of soybeans in preceding corn rows were 2.0 to 5.3 g kg^?1 higher than those from corresponding plants between corn rows. Yield of no‐till soybeans in previous corn rows increased 10 to 44% compared to those between previous corn rows. Positive impacts of prior corn rows on soil K fertility, soybean leaf K, and soybean yield occurred even when K fertilizer was not applied in the prior corn season. Deep banding of K fertilizer tended to accentuate row vs. between‐row effects on soybean leaf K concentrations in low‐testing soils. Corn row effects on soybeans were generally not affected by either tillage system or corn hybrid employed in the prior corn crop. Potassium management strategies for narrow‐row no‐till soybeans should take the potential preceding corn row impacts on soil K distribution into account; adjustments to current soil sampling protocols may be warranted when narrow‐row no‐till soybeans follow corn on soils with low to medium levels of exchangeable K.     

Aquatic Hazard Assessment of a Contaminated Surficial Aquifer Discharge into the Bush River,Maryland (U.S.A.)

An aquatic hazard assessment of contaminatedgroundwater in a surficial aquifer was conducted at Beach Pointwhich is located in the Edgewood Area of the U.S. Army Garrison,Aberdeen Proving Ground, Maryland. Toxicity was detected atvarious groundwater concentrations by seven of 10 toxicity testsystems exposed to a mixture of heavy metals and chlorinatedaliphatic hydrocarbons. When estimated maximum acceptabletoxicant concentrations (MATC) were established, the data foralgae, invertebrates, and fish suggested that the groundwaterwould not be harmful at a concentration of 10% groundwater byvolume. Likewise, no genotoxicity (Ames and SEC assays),development toxicity (FETAX), or chronic histopathology (9-monthfish test) occurred at 10% groundwater by volume.Near-field (ULINE model) and far-field (dye-tracer model)screening level dilution models were run to estimate the dilutionof the groundwater discharge plume from Beach Point into the BushRiver. The groundwater was considered to be a potentiallyexcessive hazardous material to the biota in the Bush River whena number of conservative assumptions regarding contaminantdistribution and discharge rate of the aquifer were used in thehazard assessment. By modeling the groundwater emanating fromBeach Point as the dilution of a discharge from a line diffuser,the potential water quality impacts were judged to be minimal ifState of Maryland surface water discharge criteria for a mixingzone were used for the discharge of groundwater to the Bush River.     

Behavior of myclobutanil, propiconazole, and nuarimol residues during lager beer brewing

Over a 4 month brewing process, the fate of three fungicides, myclobutanil, propiconazole, and nuarimol, was studied in the spent grain, brewer wort, and final beer product. Only the residual level of myclobutanil after the mashing step was higher than its maximum residue limit (MRL) on barley. A substantial fraction was removed with the spent grain in all cases (26-42%). The half-life times obtained for the fungicides during storage of the spent grains ranged from 82 to 187 days. No significant influence of the boiling stage on the decrease of the fungicide residues was demonstrated. During fermentation, the content reduction varied from 20 to 47%. After the lagering and filtration steps, no significant decrease (<10%) was observed in any of the residues. Finally, during storage of the beer (3 months), the amounts of fungicides fell by 25-50% of their respective concentrations in the finished beer.     

抑菌剂浇施与不均匀施肥对玉米马铃薯生长和产量的影响

农业生产中,肥料和抑菌性农药施用是两种重要的农业生产技术。在施肥过程中,点状和条状施肥是主要的施肥方式,易导致作物生长期内土壤养分以斑块状分布,因此根系趋肥性对农田中作物获取养分具有重要性。而在施用抑菌性农药时,药剂能够通过淋溶等过程进入土体中,对土壤生态环境和根-土过程产生直接或间接的影响。然而目前有关农药施用是否影响作物根系趋肥性,进而改变产量表现还不清楚。本研究选用旱地主要粮食作物玉米和马铃薯为研究对象,通过等量肥料下隔行施用的方式构建土壤养分斑块,在此基础上进行广谱性杀菌剂浇施土壤,研究抑菌性农药对作物利用异质性养分的影响。两年的大田试验数据表明,一定程度上,抑菌剂浇施和隔行施肥能够显著地影响作物的植株生物量、产量,根系生物量及分布,且对玉米生物量影响具显著交互效应,表现为隔行施肥对生物量的显著提高发生在抑菌剂浇施条件下,而抑菌剂对玉米生物量的提高则主要表现在隔行施肥条件下。同时,抑菌剂浇施能够提高作物的根系觅养精确度,其中在马铃薯上达到显著水平,表明抑菌剂浇施对作物适应土壤养分斑块具有一定的促进作用。当然,抑菌农药和养分斑块在影响作物生长过程中的显著性受作物类型和种植年份的影响,具有复杂性。因此,进一步针对不同作物、生态环境和栽培措施,探讨抑菌剂农药在作物适应养分斑块中的作用以及对作物根系趋肥的影响机制,对于了解农药施用对化肥利用的影响具有潜在的价值。     

Effect of Seaweeds on Degradation of DDT in Soils

There are concerns over the environmental risks posed by Cu-based fungicide use, and there is community and regulatory pressure on viticultural industries to restrict the use of Cu-based fungicides. This study assesses the relative environmental risks posed by Cu-based and alternative synthetic organic fungicide compounds used in Australian vineyards, giving particular consideration to their adverse effects on soil microbial activity and how risks vary across different viticultural regions. The study was guided by key steps in the ecological risk assessment framework to analyse the risks of Cu-based fungicides towards soil organisms and involved four key steps: (1) problem formulation, (2) analysis (characterise exposure and effects), (3) risk characterisation and (4) risk assessment. There is evidence of a build-up of Cu-based fungicide residues in Australian vineyard soils, although this has occurred over many years, thus allowing the availability of Cu in the soil to be attenuated over time due to aging processes. On the whole, it appears that Cu-based fungicide residues are currently unlikely to pose a significant risk to soil organisms in Australian vineyard soils. However, there are indicators that continued applications of Cu-based fungicides may well have implications on the use of impacted land for sustainable agricultural production. Further detailed studies are required to enable a more definitive characterisation of the risks posed by Cu-based fungicide residues, such as establishing a clearer link between the laboratory and agricultural settings, investigating effects on other indicators of microbial activity and biodiversity and understanding the resilience of soil microbes to additional stressors. The challenge for agricultural industries and governments, both in Australia and globally, is to formulate appropriate plans to reduce the risks associated with Cu-based fungicide use. Further research is required to consider the relative risks of a wide range of alternative fungicide compounds to ensure that they pose a lower environmental risk than the Cu-based fungicides they may replace.     

Removal of Phenol Using Sulphate Radicals Activated by Natural Zeolite-Supported Cobalt Catalysts

There is increasing community awareness of the potential environmental risks posed by Cu-based fungicide use, which is placing increasing pressure on governments and industry to undertake risk minimisation action. However, if there is going to be a widespread move away from the use of Cu-based fungicides, logically there needs to be assurance that the alternatives pose a lower environmental risk. To that end, this study compared the effect of copper hydroxide, captan and trifloxystrobin on soil enzymatic (phosphomonoesterase and urease) activity. Compared to an untreated control, copper did not inhibit either enzyme activity, even at the highest dose used in the study (156 mg/kg). At their respective high doses, captan (96 mg/kg) and trifloxystrobin (144 mg/kg) did not cause inhibition of phosphomonoesterase activity but did inhibit urease activity. Consequently, the results from this study suggest that the copper hydroxide alternatives, captan and trifloxystrobin, do not pose a short-term risk to P cycling processes in soil, although the results do suggest that these two are more toxic than copper hydroxide to N cycling processes in soil. Moreover, captan and trifloxystrobin compounds are unlikely to pose a long-term risk to soil microbial function as they are unlikely to persist in soil at concentrations found to cause an adverse effect on urease activity. Nonetheless, the potential disruption to N cycling processes needs to be recognised and consideration given to limiting the annual applications of these fungicides, particularly around the timing of repeat fungicide applications, to prevent accumulation of the fungicides in surface soils.     

蔬菜地土壤磷饱和度及其对磷释放和水质的影响

为了解蔬菜地土壤磷素的积累对水环境的影响,我们在浙江省选择了33个代表性蔬菜地,采集和分析了土壤、地表水和地下水样的磷素状况,从土壤磷饱和度的角度,研究了浙江省主要蔬菜土壤磷积累状况及其对地表和地下水水质和土壤磷释放潜力的影响。结果表明,半透膜渗析法测得的磷释放量与土壤磷积累呈正相关,磷释放量随土壤磷饱和度的提高而增加。蔬菜地土壤磷饱和度的增加可显著提高地表水体和地下水中磷的浓度,当土壤磷饱和度小于25%左右时,水体中磷浓度随土壤磷饱和度增加较为缓慢;但当磷饱和度大于25%时,水体中磷浓度随土壤磷饱和度提高迅速上升。地表水中磷浓度主要与表层土壤磷饱和度有关;地下水中磷浓度主要受深层土壤磷饱和度的影响,与表层土壤磷饱和度的相关性较小。土壤磷饱和度可很好地表征土壤磷释放和对环境的潜在影响。     

Is the application of carbendazim harmful to healthy plants? Evidence of weak phytotoxicity in tobacco.

To understand the phytotoxic effects that certain bezimidazole fungicides exert on plant growth, the aim of the present study was to determine the effect of the fungicide carbendazim, on foliar biomass, pigment content, and nutrient levels in tobacco plants (Nicotiana tabacum L. cv. Tennessee 86). The fungicide applied was carbendazim with a purity of 100%, at three different rates: 1.3 mM (carb1), 2.6 mM (the recommended concentration, carb2), and 5.2 mM (carb3). The control treatment was without carbendazim. The application of dosages of this fungicide lower than recommended (1.3 mM) resulted, on the one hand, in greater dry weight and, on the other, higher carotenoid concentrations, as well as higher N and K concentrations with respect to control. On the contrary, the application of the carbendazim dosage higher than recommended (5.2 mM) caused a decrease in dry weight and in all of the foliar pigments, as well as all of the nutrients, with respect to the other dosages and control. These results appear to indicate that besides its direct antibiotic action against pathogens, the effects of this fungicide in plants could be dangerous, especially at higher dosages. Nevertheless, the negative effects of carbendazim can be avoided by reducing the amount applied in current agriculture.     

干旱绿洲长期微咸地下水灌溉对棉田土壤微生物量影响

由于淡水资源的缺乏,利用微咸地下水灌溉是干旱绿洲普遍采用的一种灌溉措施。该文对北疆棉区长期利用微咸地下水灌溉的土壤微生物和酶活性进行了研究。结果表明,长期微咸地下水灌溉土壤的含盐量比渠水灌溉上升61.5%,显著增加了棉田耕层土壤盐分（P＜0.05）,土壤可交换性钠百分率（ESP）升高3.2倍,并造成土壤碱化。微咸地下水灌溉纤维素酶、脲酶等、转化酶及过氧化氢酶4种酶活性分别降低了21.3%、50.9%、50.0%和10.5%,但在微咸地下水灌溉条件下多酚氧化酶和碱性磷酸酶的活性显著升高。微咸地下水灌溉对土壤微生物有明显抑制作用,长期微咸水灌溉使土壤微生物量碳、氮分别降低24.4%和42.4%,但对微生物量磷影响不显著。微生物量和酶活性与棉田土壤肥力密切相关,长期微咸地下水灌溉导致有机质、全氮分别降低26.8%和28.0%。长期微咸地下水灌溉影响了土壤生物质量,不利于绿洲农田土壤的持续利用。