甲硫酸新斯的明治疗犬瘟热瘫痪后遗症

犬瘟热是由犬瘟热病毒引起的犬的一种烈性传染病.大部分病犬在病的中后期出现较为严重的神经症状,其主要表现包括咬肌痉挛性收缩,磕牙;单侧或双侧眼轮匝肌痉挛,不自主地连续不断地眨眼,头颈侧弯或后弯,观星状或角弓反张;后躯和后肢双侧或单侧麻痹,甚或高位(腰位)截瘫;全身不自主地抽搐;两前肢瘫痪或一侧前肢偏瘫等等.     

甲硫酸新斯的明治疗犬瘟热瘫痪后遗症

犬瘟热是由犬瘟热病毒引起的犬的一种烈性传染病。大部分病犬在病的中后期出现较为严重的神经症状，其主要表现包括咬肌痉挛性收缩，磕牙；单侧或双侧眼轮匝肌痉挛，不自主地连续不断地眨眼，头颈侧弯或后弯，观星状或角弓反张；后躯和后肢双侧或单侧麻痹，甚或高位（腰位）截瘫；全身不自主地抽搐；两前肢瘫痪或一侧前肢偏瘫等等。犬瘟热神经性瘫痪症状有些可随犬瘟热疾病的治愈而逐渐消失，但仍有相当多存活下来的犬瘟热病犬留下了难以逆转的瘫痪后遗症。笔者用拟胆碱药甲硫酸新斯的明试治犬瘟热瘫痪后遗症，取得较好的治疗效果，现将治疗…     

甲硫酸新斯的明治疗犬瘟热瘫痪后遗症

犬瘟热是由犬瘟热病毒引起的一种犬烈性传染病。大部分病犬在病的中后期出现较为严重的神经症状,其主要表现为咬肌痉挛性收缩,磕牙;单侧或双侧眼轮匝肌痉挛,不自主地连续眨眼,头颈侧弯或后弯,角弓反张;后躯和后肢双侧或单侧麻痹,甚至高位(腰位)截瘫;全身不自主地抽搐;两前肢瘫痪或一侧前肢偏瘫等等。犬瘟热神经性瘫痪症状有些可随犬瘟热疾病的治愈而逐渐消失,但仍有相当多存活下来的犬瘟热病犬留下了难以逆转的瘫痪后遗症。笔者用拟胆碱药甲硫酸新斯的明试治犬瘟热瘫痪后遗症,取得较好的治疗效果,现将治疗经过叙述如下:1病例白色小北京雄性…     

甲硫酸新斯的明治疗犬瘟热瘫痪后遗症

犬瘟热是由犬瘟热病毒引起的犬的一种烈性传染病.大部分病犬在病的中后期出现较为严重的神经症状,其主要表现包括咬肌痉挛性收缩,磕牙;单侧或双侧眼轮匝肌痉挛,不自主地连续不断地眨眼,头颈侧弯或后弯,观星状或角弓反张;后躯和后肢双侧或单侧麻痹,甚或高位(腰位)截瘫;全身不自主地抽搐;两前肢瘫痪或一侧前肢偏瘫等等.犬瘟热神经性瘫痪症状有些可随犬瘟热疾病的治愈而逐渐消失,但仍有相当多存     

RP-HPLC测定注射液中甲硫酸新斯的明含量

建立了反向高效液相色谱法(RP-HPLC)测定注射液中甲硫酸新斯的明的含量。采用C18柱(4.6×150 mm,5 μm),以0.1%磷酸水溶液(用三乙胺调pH至3.0)-甲醇(90：10)为流动相,260 nm为检测波长。实验结果表明,甲硫酸新斯的明在10～2000 ng.mL_-1范围内线性关系良好,R₂=0.9999,平均回收率范围为99.2%～100.8％,RSD=0.52%。该方法简便、精确、重现性好,可用于甲硫酸新斯的明注射液的质量控制。     

犬瘟热后遗症的康复治疗试验

犬瘟热是由犬瘟热病毒引起犬的一种高度接触性传染病[1],犬瘟热病毒是多嗜性病毒,其侵害神经的部位不同,临床上出现的神经症状也不相同[2],且在感染后3～4周,全身症状好转后几天至十几天出现神经症状[3].犬瘟热的神经症状表现形式多样,主要为肌肉抽搐和两后肢或整个后躯的麻痹.由于犬瘟热发病率高,死亡率高,治愈率低,即使存活下来也有相当多的会留下舞蹈病、麻痹或瘫痪等后遗症[4],而犬瘟热后遗症的康复治疗又是一个世界性的难题.长期以来,人们在犬瘟热治疗上进行了很多努力,但收效甚微.因此很有必要对犬瘟热后遗症的康复治疗进行探讨.为此我们从个案入手,开展了犬瘟热后遗症的康复治疗试验,取得了比较满意的康复治疗效果,现报告如下,仅供同行参考.     

甲硫酸新斯的明治疗犬瘟热瘫痪后遗症

犬瘟热是由犬瘟热病毒引起的犬的一种烈性传染病。大部分病犬在病的中后期出现较为严重的神经症状,其主要表现包括咬肌痉挛性收缩,磕牙;单侧或双侧眼轮匝肌     

甲硫酸新斯的明治疗犬瘟热瘫痪后遗症

犬瘟热是由犬瘟热病毒引起的犬的一种烈性传染病。大部分病犬在病中后期出现较为严重的神经症状,其主要表现包括咬肌痉挛性收缩,磕牙。单侧或双侧眼轮匝肌痉挛,不自主地连续不断地眨     

甲硫酸新斯的明治疗犬瘟热瘫痪后遗症

犬瘟热是由犬瘟热病毒引起的犬的一种烈性传染病。大部分病犬在病的中后期出现较为严重的神经症状,其主要表现包括咬肌痉挛性收缩,磕牙;单侧或双侧眼轮匝肌痉挛,不自主地连续不断地眨眼,头颈侧弯或后弯,观星状或角弓反张;后躯和后肢双侧或单侧麻痹,甚或高位(腰位)截瘫;全身不自主地抽搐;两前肢瘫痪或一侧前肢偏瘫等等。犬瘟热神经性瘫痪症状有些可随犬瘟热疾病的治愈而逐渐消失,但仍有相当多存活下来的犬瘟热病犬留下了难以逆转的瘫痪后遗症。笔者用拟胆碱药甲硫酸新斯的明试治犬瘟热瘫痪后遗症,取得较好的治疗效果,现将治疗经过叙述     

犬瘟热的治疗

犬瘟热是由犬瘟热病毒引起的一种高度接触性传染病,传染性强、死亡率高,病程较长者极容易出现愈后不良,因此快速、有效的对患犬进行治疗,是降低死亡率、减少犬瘟热后遗症的重要手段。笔者将工作中遇到的一病例的治疗情况做简要介绍。     

犬瘟热的诊治

犬瘟热俗称狗瘟,是由副黏膜病毒科麻疹病毒的犬瘟热病毒引起的一种传染性极强的病毒性传染病.由于近年来人们生活水平提高,养犬数量大幅增加,特别是城区宠物犬的饲养增加更快.根据塔城市动物防疫监督站的初步统计,目前塔城市城区饲养的各类犬有3 000多条,乡镇农村的饲养量更大.     

犬新斯的明中毒1例

一、发病情况及诊断 2007年11月6日晚10时许,城南李某带一松狮犬到我院就诊,该犬为雄性,4月龄,体重7千克.主诉:当晚8时在右,该犬出现急躁、乱跑、咬尾巴、呜叫不已,继而出现呕吐、拉稀、嘴流粘液、抽搐、狂燥不安等症状,经询问后得知,6点半左右,犬主家小孩将一包溴吡斯的明药物拿出来玩时,撒在地上,该犬误食了1包溴吡斯的明的糖衣药粒,约30多粒,才意识到犬中毒,遂来院就诊.临床检查,病犬全身抽搐、肌肉震颤、口流粘液、大小便失禁,体温为39℃.     

非典型性犬瘟热疫情的处置1例

<正>2009年7月,湖北省公安厅在襄樊市公安局警犬基地举办全省警犬技术复训班,期间发生一起犬瘟热疫情,现将发现、处置经过报道如下:一、疫情发生情况7月11日,一外地参训学员携带1头德牧工作犬到达基地报到,晚6时许该学员向省厅教员报告称其所带犬在单位已5天未进食,当地兽医以     

Non-cytocidal infection of keratinocytes by canine distemper virus in the so-called hard pad disease of canine distemper

A late, but not uncommon sequel to canine distemper virus (CDV) infection of dogs is thickening of footpads and nasal planum, the so-called hard pad disease, originally described as vacuolar degeneration of epidermal keratinocytes with inclusion body formation and massive hyperkeratosis. However, in a recent study of footpads of naturally CDV-infected dogs only hyperkeratosis was observed without any of the other changes. Instead, acanthosis was frequently noticed. CDV nucleoprotein was present in the suprabasal keratinocytes and eccrine epithelial glands only. No CDV nucleoprotein was present in basal keratinocytes. This observation in combination with lack of obvious cytocidal changes strongly suggested the possibility of a restricted viral infection with presence of viral mRNA but without protein expression. Therefore, the presence of CDV nucleoprotein mRNA was investigated using in situ hybridization and compared to the localization of the nucleoprotein in footpads of clinically healthy and distemper dogs. Viral nucleoprotein and nucleoprotein mRNA in nearly all cases co-localized to the same compartments and basal keratinocytes did not contain nucleoprotein mRNA. These findings dispute the idea of a restricted viral infection of footpad keratinocytes in dogs with natural CDV infection. Instead, a migration of the virus to the epidermal surface along with the proliferating and differentiating epithelium is the most likely explanation for the lack of virus antigen in basal keratinocytes.     

Immunohistochemical demonstration of canine distemper virus antigen as an aid in the diagnosis of canine distemper encephalomyelitis

Brain tissue from 33 dogs with non-suppurative encephalitis was examined for evidence of canine distemper virus (CDV) encephalitis. Sections were examined for lesions, inclusion bodies, syncytial cells and CDV antigen using a double bridge unlabelled antibody enzyme technique. Histopathological lesions considered to be typical of granulomatous meningoencephalomyelitis were found in seven dogs. They all lacked inclusion bodies, syncytial cells and CDV antigen. The remaining 26 dogs all had histopathological lesions typical of CDV encephalitis. Inclusion bodies were found in 24 dogs, four of which also had syncytial cells and CDV antigen was detected immunocytochemically in 25. One dog had no inclusion bodies or syncytial cells and was immunohistochemically negative. Syncytial cells have been found to be of limited diagnostic value for the diagnosis of CDV encephalitis. While inclusion bodies proved to be a good diagnostic criterion for the confirmation of CDV infection, the immunohistochemical demonstration of CDV antigen proved to be superior. CDV antigen was more prevalent than inclusion bodies in tissue sections and much more easily detectable.     

国内流行犬瘟热病毒DNA重组疫苗的初步研究

为了探究H蛋白的免疫原性以及抗体效价,构建了1株犬瘟热病毒(CDV)ZH-10株H基因的真核表达载体pCI-H,对表达产物的免疫原性进行了初步研究,并进行了小鼠的DNA免疫试验。结果显示,pCI-H免疫组血清可与感染病毒的MDCK细胞发生特异性IPMA反应呈现特异的棕红色;ELISA血清抗体滴度可达1:28~1:79;病毒中和抗体可达1:11~1:32。研究结果表明,H蛋白具有免疫原性,但诱导抗体效价不足,需进一步完善。