Assessment of contrasting perennial ryegrasses, with and without white clover, under continuous sheep stocking in the uplands. 3. Herbage production, quality and intake

Herbage characteristics were monitored over 3 years (1985–1987) in three perennial ryegrass ( Lolium perenne L.) varieties as grass/only (200 kg N ha⁻¹) and grass/clover ( Trifolium repens L.) (75 kg N ha⁻¹) swards when continuously stocked with sheep. Although mean total annual herbage production was similar from the grass varieties, growth of Aurora, a very early-flowering variety, was higher than that of Aberystwyth S23 and Meltra (tetraploid) late-flowering types in spring. Herbage production from grass-only swards was 15% higher than from grass/clover swards.
In vitro organic matter digestibility (OMD) of Aurora and Meltra was 34 g kg ⁻¹ and 26 g kg ⁻¹ higher ( P < 0·01) than that of S23. Averaged over varieties, the OMD of grass/clover was 25 g kg ⁻¹ higher ( P < 0·05) than grass-only during the post-weaning period. Individual lamb digestible organic matter intake (DOMI) was 22% higher ( P < 0·05) on grass/clover than on grass-only during this period. Water-soluble carbohydrates content of Aurora was 42% higher ( P < 0·001) than that of both Meltra and S23.
Herbage characteristics were related to lamb performance reported previously. Lamb production was positively correlated with DOMI. However, the magnitude of differences in lamb performance between treatments was much greater than the extent of differences detected in herbage characteristics.     

Assessment of contrasting perennial ryegrasses, with and without white clover, under continuous sheep stocking in the uplands

Comparative sheep production from Aurora (very early-flowering), Meltra (late-flowering tetraploid) and Aberystwyth S23 (late-flowering) perennial ryegrass ( Lolium perenne L.) varieties was assessed as both grass-only (200 kg N ha⁻¹) and grass-white clover ( Trifolium repens L.) (75 kg N ha⁻¹) swards under continuous stocking management. Beulah Speckled Face ewes and their Suffolk cross lambs were used from late April to mid-July. From late July to early November only lambs grazed the pastures.
Mean (1985–87) total annual lamb production per hectare from Aurora and Meltra was 16% and 13% more than that from S23. The magnitude of the differences between these varieties and S23 declined from the first to the third year. Aurora gave 29% more lamb output per hectare than S23 in 1985 but only 10% more in 1987, while the advantage of Meltra over S23 fell from 19% in 1985 to 6% in 1987. Seasonal lamb production per hectare varied between the grasses, especially during spring when lamb output from Aurora was 43% and 22% more than that from S23 and Meltra respectively, with that from the tetraploid being 17% higher than that from S23.
It is concluded that the superior spring output from Aurora offers the farmer the opportunity to reduce his dependence on bought-in feedstuffs, hence improving the efficiency and profitability of lamb production from grassland.     

Assessment of contrasting perennial ryegrasses, with and without clover, under continuous sheep stocking in the uplands. 4. Animal performance in years 4–6

Relative sheep production from Aurora (very early flowering), Mellra (late-flowering tetraploid) and Aberystwyth S23 (late flowering) perennial ryegrass ( Lolium perenne L.) varieties was compared during harvest years 4-6 (1988-90) as grass only (200 kg N ha⁻¹) and grass/clover (75 kg N ha ⁻¹ in 1988, 0 kg N ha⁻¹ in 1989 and 1990) at Bronydd Mawr Research Centre (310-363 m) in mid-Wales. The pastures were continuously stocked with Beulah Speckled Face ewes and their Suffolk cross lambs from spring to mid-July. From late July to early November only the weaned lambs grazed the swards.
Mean (1988-90) total annual lamb production per hectare from Aurora and Meltra was 70% and 16% more than that from S23. However, the difference between both varieties and S23 was much greater as grass/clover (Aberystwyth S184 small-leaved type) than as grass-only swards. Differences between the grasses in individual lamb growth rates were most pronounced during the post-weaning period, when that on Meltra was 38% and 23% more than on Aurora and S23 respectively.
Mean annual lamb output from grass/clover swards was only 70% of that from grass-only swards during the 2 years when the former swards received no fertilizer N. Averaged over the 3 years (1988-90) individual lamb liveweight gain post weaning was 38% higher on grass/clover than on grass-only swards.
The results are discussed in relation to those for the first 3 harvest years (1985-87). It is concluded that, although Aurora was less persistent than both late-flowering varieties, its superiority in providing an average of 29% more lamb output than S23 during the critical spring period (up to early June) would be of considerable practical significance in upland sheep systems, as would be the overall superiority of the tetraploid Meltra over S23.     

A comparison of diploid and tetraploid perennial ryegrass and tetraploid ryegrass/white clover swards under continuous sheep stocking at controlled sward heights. 3. Sward characteristics and animal output, years 4–8

Results for years 4–8 of a long-term grazing experiment on swards of a diploid perennial ryegrass (Lolium perenne), var. Contender (D swards), a tetraploid ryegrass, var. Condesa (T swards) and Condesa with S184 white clover (Trifolium repens) (TC swards), direct sown in May 1987, are presented. The swards were continuously stocked with sheep from 1988 to 1990, as previously reported, and for a further 5 years, 1991–95, at a target sward surface height (SSH) of 4–6 cm. Control of sward height was successfully achieved by variable stocking, except in 1993 when paddocks were set stocked and the resulting mean SSH was 9·3 cm. Grass swards received on average 160 kg N ha^?1 year^?1; grass/clover swards were mainly not fertilized with N with the exception that they were given 30 kg N ha^?1 as a remedial mid-summer application during a period of low herbage mass on offer in 1994 and 1995. Mean white clover content of the swards fell from 18·2% of herbage dry-matter (DM) in 1992 to 8·5% in 1993, whereas stolon lengths fell from 120 to 58 m m^?2. A return to lower sward heights in 1994–95 resulted in an increase in white clover content to 12·8% by the final sampling in August 1995. Perennial ryegrass content of the grass swards remained high throughout (mean 96·7% in 1995). Perennial ryegrass tiller densities recorded in August 1991, 1993 and 1994 showed consistently significant (P < 0·001) sward differences (3-year mean 16 600, 13 700 and 10 100 perennial ryegrass tillers m^?2 for the D, T and TC swards). In 1994, the year after lax grazing, a low perennial ryegrass tiller density (9100 m^?2) and low white clover content (mean 4·3%) in the TC swards resulted in a much lower herbage bulk density than in the grass swards (April–July means 72, 94 and 44 kg OM ha^?1 cm^?1 for the D, T and TC swards). There was a consistent 40 g d^?1 increase in lamb liveweight gain on the TC swards over the T swards, except in 1994. In that year there was a reduction in lamb liveweight gain of 33 g d^?1 on the TC swards and a significant increase in ewe liveweight loss (117 g d^?1) associated with low herbage bulk density despite optimal sward height. Lamb output (kg liveweight ha^?1) on TC swards reflected white clover content, falling from a similar output to that produced from grass given 160 kg N ha^?1, at 18% white clover DM content, down to 60% of grass + N swards with around 5% clover. A 6% greater output from the T than the D swards was achieved mainly through higher stocking rate. The experiment demonstrated a rapid, loss in white clover under lax grazing, and showed that the relationship between performance and sward height is also dependent on herbage density. High lamb output from a grass/clover sward was only achieved when the clover content was maintained at 15–20% of the herbage DM.     

Digestion in vitro of Italian and perennial ryegrasses, red clover, white clover and lucerne

Herbage at various stages of growth from three field experiments, one with Italian ryegrass, one with perennial ryegrass and one with white clover, red clover and lucerne, was incubated for 0, 24 or 48 h in rumen liquor followed by 48 h in acid-pepsin. The proportion of cell content OM in herbage (measured by pepsin) declined with advancing stage of growth more quickly in the legumes than in Italian ryegrass. At a high level of digestibility the legumes had a higher proportion of cell content OM than Italian ryegrass, but the position tended to be reversed at a low level of digestibility. Decline in proportion of cell content and decline in digestibility of cell wall were both major contributors to the decline in digestibility of Italian ryegrass with advancing maturity, whereas in white clover the decline in proportion of cell content was entirely responsible for the decline in digestibility. Lucerne and red clover occupied intermediate positions. At an early vegetative stage rate of digestion appeared to be higher in Italian than in perennial ryegrass. Rate of digestion was higher in the legumes than in the ryegrasses, the difference being largest at an advanced stage of growth.     

A comparison of diploid and tetraploid perennial ryegrass and tetraploid ryegrass/white clover swards under continuous sheep stocking at controlled sward heights. 2. Animal production

Performance of continuously stocked Mule ewes nursing Suffolk-cross twin lambs over three grazing seasons, between April and August, was compared on swards of N-fertilized diploid perennial ryegrass (D), tetraploid perennial ryegrass (T) and tetraploid perennial ryegrass with white clover (TC), the latter receiving no fertilizer N. Sward height was maintained by variable stocking rate close to a target of 4–6 cm (constant treatment) from turnout and compared in July and August with a rising sward height treatment (target 6–8 cm). Lambs on TC swards had significantly higher (P <0·001) liveweight gains compared with lambs on T swards by 41 gd^-1 in April–June and by 68gd^-1 in July-August. Live weight and body condition score of ewes in August were significantly higher (P<0·001) on TC compared with T swards, by 11·3 kg and 0·75 respectively. Rising sward heights in July–August increased live-weight gain of lambs compared with constant sward heights by 102, 39 and 54gd^-1 in consecutive years, associated with sward height increases of 0·9, 0·5 and 0·6cm respectively. Rising sward height increased ewe live weight and body condition score by 5·1 kg and 0·3 respectively, compared with results from constant sward heights. Effects of sward height and sward type were additive. T swards had a significantly (P<0·01) 16% greater overall lamb output than the D swards due mainly to a 10% higher achieved stocking rate. Stocking rates of ewes on TC vs T swards were 40, 13 and 12% lower in April-August in successive years. The higher liveweight gain of lambs on the TC swards resulted in lamb outputs of 76, 105 and 101% of the T swards in successive years, showing that grass/clover swards containing over 20% clover could produce similar lamb output ha^-1 to grass swards given 150–180 kg N ha^-1.     

Herbage intake and milk production by grazing dairy cows. 3. The effect of grazing severity under continuous stocking

The effects of severity of grazing on the herbage intake and milk production of continuously stocked British Friesian cows calving in February–March were examined in three experiments conducted in the years 1976–78 (experiments 1–3 respectively) using a put-and-take technique. In experiment 1 four grazing severities were imposed by maintaining swards with different herbage masses (2500, 3000, 3500, 4000 kg OM ha^-1); in experiments 2 and 3 there were two severities of grazing maintained by keeping swards canopies at constant heights of 5 and 7 cm (experiment 2) and 5 and 7·2 cm (experiment 3). Cows were reallocated to treatment every 8 weeks in experiments 1 and 2 and there were three periods, whereas they all grazed throughout a 23-week period on the same treatment in the final trial.
A decrease in the quantity of herbage on offer or in sward height reduced herbage intake and milk production in all experiments. Mean daily herbage OM intakes were 11·2, 12·2, 12·2 and 12·2 kg respectively in experiment 1, 12·2 and 13·2 kg respectively in experiment 2 and 12·2 and 152 kg respectively in experiment 3. Mean daily solids–corrected milk yields were 14·2, 15·2, 15·2 and 16·2 kg respectively in experiment 1, 14·2 and 16·2 kg respectively in experiment 2 and 12·2 and 17·2 kg respectively in experiment 3. It was apparent from the data obtained in the first two trials that grazing at a sward canopy height of 7 rather than 9 cm had little effect, but that at 5 cm there were significant depressions in both herbage intake and milk production. Milk yield was depressed to a greater extent when cows were kept on the same treatment for the whole season.     

The effect of blending white clover varieties and their contribution to a mixed grass/clover sward under continuous sheep stocking

The effect of blending small- and medium-leaved white clovers together in a mixture of varieties was examined under continuous sheep stocking over a period of three years. Four varieties were used, S184 and Gwenda, small-leaved varieties suitable for intensive sheep grazing, together with medium leaved varieties Menna and Donna, which are mainly used in general purpose seed mixtures for medium term leys. The small-leaved varieties were blended with Menna or Donna, sown with a commercial grass mixture and managed as near as possible to farm practice. Although the leaf size of Gwenda is only slightly greater than that of S184, blends based on these two small-leaved varieties behaved differently. In spring of the first harvest year the yield of Gwenda and of the clover in mixtures containing Gwenda was 27% greater than the yield of the same mixtures which contained S184. As the season progressed this difference decreased. In terms of total annual yields and saving in fertilizer N, the benefits of including white clover in a seed mixture were more pronounced when Menna was mixed with S184 and Gwenda, rather than when Donna was used, although both varieties are in the medium-leaf category. It was concluded that the slower establishment and the lower clover yield of S184, when compared with larger leaved varieties, can be overcome by blending with a variety that is slightly larger in leaf size, such as Menna, although the choice of variety may depend on sward management and its persistency under grazing.     

A comparison of diploid and tetraploid perennial ryegrass and tetraploid ryegrass/white clover swards under continuous sheep stocking at controlled sward heights. 1. Sward characteristics

Two 1·0 ha plots of a late-heading diploid perennial ryegrass (var. Contender) and a late-heading tetraploid ryegrass (var. Condesa), and two 1·4 ha plots of the tetraploid with Aberystwyth S184 small-leaved white clover, were direct sown in May 1987. Over the three years 1988–90 they were continuously stocked by Mule ewes with Suffolk-cross twin lambs, from early April to the end of August, at a target sward surface height (SSH) of 4–6 cm on one set of plots (constant swards) and, on the other set, al 4–6 cm rising after June to a target 6–8 cm (rising swards). The heights were achieved by variable stocking. Fertilizer N was applied only to the grass plots at the rate of 150- 180kgN ha^-1 annually.
SSH was mainly within the target 4–6 cm, after higher initial heights at turnout in 1988and 1990. Mean heights of the constant swards (April- August) averaged 5·53, 4·43 and 5·04cm in the three years. The rising swards (July-August) increased in height over the constant swards by an average of 0·88, 0·48 and 0·55 cm, in successive years.
Clover content of the herbage mass dry matter in the grass/clover swards increased over each grazing season to average 13·0, 26·5 and 21·2% in the three years, with a high mean stolon density of 130 in m^-2 in August 1990. Ryegrass tiller densities in year 3 were 23% higher in the diploid than in the tetraploid swards, which had 43% more than the 10000 tillers m^-2 of the tetraploid ryegrass/clover swards.
It is concluded that the combination of a densely stoloniferous small-leaved clover with the open growth habit of a tetraploid ryegrass can achieve swards of high clover content under continuous sheep stocking.     

Effects of continuous sheep stocking and strategic rest periods on the sward characteristics of binary perennial grass/white clover associations

Five binary perennial grass/white clover (Trifolium repens, cv. Menna) mixtures were evaluated over a 3-year period under continuous sheep stocking together with the imposition of a rest period for either an early or a late conservation cut; the experiment with plot sizes of 0·16 ha was replicated three times. The grass species and cultivars used were Merlinda tetraploid and Magella diploid perennial ryegrass (Lolium perenne), Prairial cocksfoot (Dactylis glomerata), Rossa meadow fescue (Festuca pratensis) and Goliath timothy (Phleum pratense). The greatest total lengths of white clover stolon developed in the meadow fescue (171·6 m m^?2) and timothy (151·9 m m^?2) associations compared with those in tetraploid perennial ryegrass (98·6 m m^?2), diploid perennial ryegrass (91·9 m m^?2) and cocksfoot (74·6 m m^?2) (s.e.d. 16·4, P < 0·001). On average, the proportion of white clover stolon that was buried was between 0·86 and 0·89 and this was more abundant in late than early season. Whereas timothy persisted, the persistence of meadow fescue was low under any of the managements tested and this was markedly reduced by the third grazing season. In the diploid perennial ryegrass sward, a late June to early August rest period for conservation enhanced white clover stolon length. An early April to late May rest period greatly reduced total white clover stolon length in both diploid perennial ryegrass and tetraploid perennial ryegrass associations (diploid perennial ryegrass-unrested 89 m m^?2, early rest 56·1 m m^?2, late rest 130·7 m m^?2; tetraploid perennial ryegrass - unrested 125·1 m m^?2, early rest 71 m m^?2, late rest 99·7 m m^?2; s.e.d. 19·19, P < 0·001). The numbers of white clover stolon growing points per unit stolon length were greatest when the sward was rested during late June to early August ?55·9 m^?1 stolon length compared with 45·7 m^?1 for an April to late May rest and 46 m^?1 in the absence of a rest (s.e.d. 2·59, P < 0·001). Likewise, the percentage of stolon above ground was greatest with the late June to early August rest ?15·78% compared with 10·61% for the April to late May rest and 7·69% for no rest (s.e.d. 1·569, P < 0·001). The complementary percentages of buried stolon indicate the important role this fraction has and the need to study stolon behaviour in grazing studies generally. It is concluded that, in relation to perennial ryegrass as a companion grass, meadow fescue and timothy allow better white clover development and cocksfoot less. However, other attributes have to be considered, for example the poor persistence of meadow fescue and the slower regrowth of timothy, both of which allow the invasion of weed grasses, or the lower acceptability of cocksfoot to livestock. The timing of the rest period before the conservation cut can influence white clover development considerably, but the effects differed with different companion grasses.     

Relationships between morphological and chemical characteristics of perennial ryegrass varieties and intake by sheep under continuous stocking management

Plots of five intermediate‐heading varieties of perennial ryegrass (Lolium perenne L.) [AberDove, Belramo and Glen (diploid); Twins (tetraploid); and AberExcel (tetraploid hybrid)] were continuously stocked with sheep to maintain a target sward surface height of 40–50 mm. Daily dry matter (DM) intake was significantly different (F‐value = 0·032) between the varieties, with the tetraploid hybrid AberExcel having the highest values for daily DM intake and intake rate during eating. Amongst the diploid varieties, intake rate tended to be higher for sheep grazing Glen. The varieties comprised a wide range in potential growth habit, from the relatively prostrate, highly tillered Glen to the more‐erect AberExcel and there were differences between them in the vertical distribution of leaves within the sward canopy. The leaves of AberExcel weighed 3·6 mg DM cm^?2 leaf area in contrast to the other varieties (4·3–5·3 mg DM cm^?2 leaf area) resulting in a high leaf area index (LAI) in relation to the green leaf mass. Intake rate was not significantly correlated with extended tiller and sheath tube lengths, partition of herbage mass, number of tillers per square metre or LAI. However, canonical variates analysis showed that there were significant differences between the varieties for the morphological and chemical factors examined. Other factors also need to be explored to explain these differences in ingestive behaviour in order to identify plant traits that are correlated with herbage intake rate. These are needed for varieties destined for grazing use, both during the breeding programme and their subsequent evaluation.     

Relationships between the yield of perennial ryegrass and of small-leaved white clover under cutting or continuous grazing by sheep

Seven varieties or advanced breeding lines of white clover ( Trifolium repens L.), all of small leaf size, were grown separately in mixtures with perennial ryegrass ( Lolium perenne L.) in an experiment encompassing three harvest years. Harvestable dry-matter (DM) yield measurements were taken of these mixtures and of perennial ryegrass monocultures under two management regimes: cutting and continuous sheep grazing. Considerable differences were observed in the harvestable DM yields of white clover, perennial ryegrass and total yields of the mixtures between plots containing different white clover varieties. White clover yields were generally higher under cutting, and perennial ryegrass yields were higher under grazing. The difference between perennial ryegrass yield in monoculture and in mixture was variable. In the second harvest year, a significant interaction effect was seen between management and white clover variety for white clover yield but not for perennial ryegrass yield. The relationship between clover yield and grass yield differed between the two management regimes. Under cutting, a negative correlation was observed, indicative of competitive effects. However, under grazing, no such correlation was seen. Possible mechanisms underlying these outcomes are discussed.     

Associative effects between fresh perennial ryegrass and white clover on dynamics of intake and digestion in sheep

The aim of this study was to determine the associative effects between fresh perennial ryegrass and fresh white clover on intake and digestion in sheep. Five proportions of ryegrass and white clover, ranging from 0 to 1 in 0·25 increments (DM basis), were allocated to two groups of five sheep according to a replicated 5 × 5 Latin square design, and intake behaviour and digestive parameters including methane (CH₄) emissions were measured. Feeding behaviour was affected through increased forage intake when the proportion of white clover was increased, and there were positive associative effects on intake, eating time and eating rate during the main meals consumed immediately after feed was given. These effects seemed to be related to a greater motivation to eat, rather to more efficient digestion, as no difference on DM digestibility (average 766 g kg^?1) was detected. There were no differences in CH₄ yield (average 18·1 g kg^?1 DM intake) among the treatments. The optimal proportion of white clover fed with ryegrass to optimize intake and digestion was between 0·25 and 0·50. These results indicate that mixing perennial ryegrass and white clover positively affects forage intake in sheep and their motivation to eat.     

Competition in perennial ryegrass–white clover mixtures under cutting. 2. Leaf characteristics, light interception and dry-matter production during regrowth

The effect of defoliation interval on growth patterns of contrasting perennial ryegrass (Lolium perenne)–white clover (Trifolium repens) mixtures was studied. The dynamics of increase in leaf area, light interception and dry-matter (DM) production were measured within successive regrowth periods. No N fertilizer was applied. During 1995 six mixtures were cut eight (F1) or six times (F2) at a stubble height of 5 cm. The stubble composition was stable throughout the growing season: after harvest about 50 g DM m^?2 (with a white clover proportion of 0·52) was present with a leaf area index (LAI) of 0·5 (0·38 white clover). The percentage of intercepted radiation after cutting was 20–30% and increased during 3 weeks to about 95%. The relative growth rate of leaf area and DM was higher for white clover than for perennial ryegrass, with the proportion of clover in the LAI and DM increasing during each regrowth period. Mixtures with large-leaved white clover cv. Alice had a lower initial clover content after harvest, but a more rapid increase in clover LAI and DM than mixtures with the smaller leaved cvs Gwenda or Retor. Alice had the highest total and clover LAI and DM at harvest. Cutting frequency affected the change in white clover–perennial ryegrass ratio during regrowth. This was significantly higher in mixtures with Alice than in mixtures with Gwenda, but only under less frequent cutting (F2). In spring there was a mean white clover proportion of about 0·55 in the LAI and 0·45 in the total harvested DM. In summer the white clover proportion in the LAI and DM increased to 0·70–0·75. There was a decline during autumn, especially in F2 and in the mixtures with the small-leaved white clover cv. Gwenda and the medium-leaved cv. Retor. In contrast, grass DM and LAI declined from spring to summer. The decline in clover LAI in autumn was similar in Alice and Gwenda at frequent cutting (F1), but stronger in Gwenda in F2. Retor had the lowest clover specific leaf area (SLA). The SLA values of Alice and Gwenda were similar, SLA being similar between cutting treatments. No differences were found for leaf weight ratio (LWR) among the three white clover cultivars or between the grass cultivars, and LWR was not affected by cutting treatment. Defoliation interval had limited effects on the growth pattern and leaf characteristics of perennial ryegrass–white clover mixtures.     

Performance of white clover varieties combined in blends and alone when grown with perennial ryegrass under sheep and cattle grazing

Abstract Two experiments were carried out to evaluate the performance of blends of three white clover (Trifolium repens L.) varieties in comparison with the component varieties and three other varieties sown individually in a mixture with perennial ryegrass (Lolium perenne L.). The plots were grazed rotationally in Experiment 1 by cattle and sheep and in Experiment 2 by sheep alone. In both experiments, the blend was composed of three medium‐leaved varieties (AberDai, AberVantage and AberHerald), but with different relative contributions of the three varieties in the two experiments. Dry matter (DM) yields of white clover and perennial ryegrass were assessed in replicate plots for two years (1999 and 2000) after the establishment year. In Experiment 1, there was no significant difference between the DM yields of white clover or perennial ryegrass in either year. The decline in DM yield of white clover between years that was observed for some varieties was not found for the blend. In Experiment 2, significant differences were found in DM yields of white clover in both years. In 1999, AberDai had the highest DM yield. In 2000, AberHerald and AberVantage had the highest DM yields, and AberDai showed a decline in DM yield that was mirrored by the mean for all the white clover varieties. In both experiments, the blend did not show significantly higher DM yield than one or more of its components; indeed, in Experiment 2, it was significantly lower yielding than AberDai in 1999. However, where one component of the blend declined in DM yield between years, this was compensated for by an increase in the yield of another component. These preliminary findings suggest that the yield stability of blends may give them a potential role in agricultural practice.     

Animal production evaluation of herbage varieties. 1. Comparison of Aurora with Frances, Talbot and Melle perennial ryegrasses when grown alone and with clover

Comparative herbage characteristics and sheep production (ewes and lambs until weaning and lambs thereafter) from swards of Aurora (very early flowering), Frances (early flowering), Talbot (intermediate flowering) and Melle (late flowering) varieties of perennial ryegrass ( Lolium perenne ) were studied under a continuous variable stocking management based on sward surface height guidelines. The varieties were assessed as grass-only (215 kg N ha⁻¹ fertilizer) and grass/clover (83 kg N ha⁻¹ fertilizer) pastures.
Over 2 years (1985-86) total annual lamb production per hectare from grass-only swards of Aurora was 19% more than that from Frances despite similar herbage productivity. Differences between the varieties in lamb output were more pronounced as grass/clover swards, with Aurora producing 29 and 18% more lamb than Melle and Talbot respectively and with Frances also giving 16% more than Melle. Overall lamb production from grass/clover swards was 10% more than that from the grass-only pastures, mainly due to 69% advantage in individual lamb growth rates after weaning. Herbage organic matter digestibility, during the post-weaning period, was higher on grass/clover than on grass-only swards, and similarly with Aurora versus the other varieties. Under a frequent cut simulated grazing regime the relative herbage productivity of the four varieties differed, with Aurora 13% less productive than Frances.
The results are discussed in relation to the limitations of assessing performance under cutting, and the significance of the interaction in animal productivity between the two sward types.     

Competition in perennial ryegrass–white clover mixtures under cutting. 1. Dry-matter yield, species composition and nitrogen fixation

Persistence of white clover (Trifolium repens) in mixtures was studied in a long-term experiment. Mixtures of two cultivars of perennial ryegrass (Lolium perenne) with contrasting growth habits and three white clover cultivars differing in leaf size were sown in 1991 and evaluated at two cutting frequencies. During 1995 and 1996 mixtures with large-leaved white clover cv. Alice had the highest dry-matter (DM) content, clover and N yield, and the highest white clover content, and mixtures with medium-leaved Retor the lowest, whereas mixtures with small-leaved Gwenda yielded most grass DM. In 1995 averaged over cutting treatments and mixtures, the mixtures yielded 11·8 t DM ha^?1 with a white clover content of 0·6; the apparent N fixation was 393 kg N ha^?1. In 1996 these values declined to 8·5 t DM ha^?1, 0·48 white clover and 236 kg N ha^?1. There was no significant effect of cutting frequency on DM yield or white clover content, whereas the effects of grass cultivar were not consistent. In spring there was a peak in the DM production of the mixtures, coinciding with a peak in production of the grass component. However, in summer and autumn the seasonal pattern of DM production of the mixtures was similar to that of the white clover component. Both cultivars of perennial ryegrass showed the same seasonal response, but the seasonal growth pattern of white clover differed slightly between clover cultivars and cutting treatments. In later years only one cutting frequency was imposed, and no yield measurements were taken. White clover was judged to have performed well during 1997; the clover content in September was very high (0·76), whereas in October 1998 it was 0·45. Mixtures with Alice contained most white clover. Despite fluctuations in white clover content during 1991–98, all clover cultivars had persisted 7 years after sowing, irrespective of companion grass cultivar, at both cutting treatments.     

The effects of sward height and nitrogen fertilizer application on changes in sward composition, white clover growth and the stock carrying capacity of an upland perennial ryegrass/white clover sward grazed by sheep for four years

Perennial ryegrass/white clover pastures grazed by sheep and receiving either no fertilizer N (No) or 120 kg N ha^?1 year^?1 (N₀) were maintained with surface heights of 2·5, 3·5 and 5·0 cm for over four years. The treatments were replicated. The white clover (WC) population was greatest in the N₀treatment, and declined during the study. Between-year variation in WC was negatively related to rainfall and positively related to temperature, WC as a proportion of the total plant population decreased during the summer in the Nl treatment. The perennial ryegrass (PRG) population was greater in the Nl treatment, declined during the study and both within and between years was positively related to temperature. The population density of the unsown grasses was highest in the N-fertilized treatment and in the swards maintained at the lowest heights (these treatments also had the highest stocking rate); it increased during the study, within-years being positively related to temperature and between-years being positively related to rainfall. The WC stolon extension rate was largely unaffected by N fertilizer application and was greatest in the taller swards. Leaf appearance rate was unaffected by N fertilizer application and sward height; it was positively related to temperature and negatively related to rainfall. Branching rate was greater in the N₀ treatment with significant sward height effects confined to a negative relationship with local sward height within treatment plots on one occasion; it was negatively related to rainfall. The ground level red:far red light ratio was negatively related to local sward height. The total live weight of sheep carried in the No treatments was 0·7 of that in the N₁ treatments. Expected photomorphogenic responses by we were confined to stolon extension. It was concluded that on the poorly drained clay-loam soil used in this study the effects of sheep, in interaction with climatic factors, had an overriding effect on clover branching rate and the ultimate species composition.     

Effect of continuous stocking by sheep at four sward heights on herbage mass, herbage quality and tissue turnover on grass/clover and nitrogen-fertilized grass swards

The effects of continuous stocking by sheep at sward surface heights (SSH) of 3, 5, 7 and 9 cm in grass/clover (GC) and nitrogen-fertilized grass (GN) swards were examined in relation to herbage mass and quality, clover content, tiller density and rates of herbage production and senescence in two periods in each of three grazing seasons (1987-89). The GN swards received a total of 300 kg N ha^?1 each year in six equal dressings from March; GC swards received a single dressing of 50 kg N ha^?1 in March each year. Herbage mass measured from ground level increased linearly with SSH with overall mean herbage masses of 0·89, 1·38, 1·78 and 2·12 t OM ha^?1 (s.e.m.0·024, P < 0·001) at SSH of 3, 5, 7 and 9 cm respectively. GN and GC swards had mean herbage masses of 1·58 and 1·51 t OM ha^?1 (s.e.m. 0·051, NS) respectively. Mean N content of herbage on GN swards was greater than that on GC swards and declined with increasing SSH. Crude, fibre (CF) content of herbage was similar for both sward types and increased with increasing SSH. Clover content of GC swards remained low throughout the experiment, ranging from 0·002 to 0·074 of herbage mass. However, from tissue turnover rates it was estimated that its contribution to herbage production was in the range of 0·049–0·219 of net herbage growth. Total growth increased with increasing SSH in both sward types, with maximum growth rates in GN swards of 143 and 130 kg DM ha^?1 d^?1 and in GC swards of 88·2 and 85·4 kg DM ha^?1 d^?1 in Periods 1 (up to early July) and 2 (after July) respectively. Senescence rates ranged between 13·3 and 50·1 kg DM ha^?1 d^?1 and tended to be higher in Period 2 than in Period 1. Net production increased with increasing SSH in Period 1, while in Period 2 net production declined at SSH above 6·5 cm. The increased net herbage production in taller swards was not associated with greater utilized metabolizable energy production at sward heights above 5 cm.     

Herbage and nitrogen yields,fixation and transfer by white clover to companion grasses in grazed swards under different rates of nitrogen fertilization

In grass–legume swards, biologically fixed nitrogen (N) from the legume can support the N requirements of the grass, but legume N fixation is suppressed by additional fertilizer N application. This study sought to identify a fertilizer N application rate that maximizes herbage and N yields, N fixation and apparent N transfer from white clover to companion grasses under intensive grazing at a site with high soil‐N status. During a 3‐year period (2011–2013), swards of perennial ryegrass and of perennial ryegrass–white clover, receiving up to 240 kg N ha^?1 year^?1, were compared using isotope dilution and N‐difference methods. The presence of white clover increased herbage and N yields by 12–44% and 26–72%, respectively. Applications of N fertilizer reduced sward white clover content, but the effect was less at below 120 kg N ha^?1. The proportion of N derived from the atmospheric N fixation was 25–70%. Nitrogen fixation ranged from 25 to 142 kg N ha^?1 measured using the isotope dilution method in 2012 and from 52 to 291 kg N ha^?1 using the N‐difference method across all years. Fertilizer N application reduced the percentage and yield of fixed N. Transfer of N from white clover to grass was not confirmed, but there was an increased N content in grass and soil‐N levels. Under intensive grazing, the maximum applied N rate that optimized herbage and N yields with minimal effect on white clover content and fixation rates was 60–120 kg N ha^?1.