利用酵母单杂交筛选拟南芥AtSTK基因表达调控蛋白的研究

拟南芥丝氨酸/苏氨酸蛋白激酶基因AtSTK的过量表达可以明显提高拟南芥的耐盐性。为进一步研究AtSTK基因表达的调控机制,以拟南芥基因组DNA为模板设计引物,扩增获得AtSTK基因的启动子序列,并构建到报告载体pAbAi,将重组报告载体质粒pAbAi-HYT利用BstbⅠ进行单酶切线性化,转化酵母菌株Y1H Gold,线性化的pAbAi-HYT整合到基因组中。然后,将纯化的拟南芥双链cDNA、pGADT7-Rec载体共转化含有报告载体pAbAi-HYT的酵母菌,将菌液涂布到含有100 ng/m L Ab A的SD/-Leu培养基上进行阳性克隆的酵母单杂交筛选。通过回复鉴定、序列测定,最终筛选获得了可能参与AtSTK基因表达调控的4个拟南芥基因。测序结果表明,酵母单杂交筛选获得的拟南芥基因AT3G32090含有WRKY转录因子保守结构域,为WRKY类转录因子的一员。因此,AtSTK基因的表达很可能接受MAPK信号传导途径中AT3G32090基因编码的WRKY类转录因子的调控,从而影响拟南芥植株的耐盐性。     

A Control Algorithm to Regulate the Active and Reactive Power and Simulation Research of Dual-frequency Dual-Buck Inverter

This paper deduceS the control algorithm ,which can both regulate the active and reactive power of the novel dual-frequency dual-Buck inverter, and the control function. The control function based on one cycle control can control the AC-side active and reactive power by simple circuit. The simulation is given and the results prove the validity of the theory analysis. This inverter is valuable for the research of grid-connected inverters.     

Discussion on Water Supply Installation with One Household One Water Meter

Along with the development of commodity economy, the charge way as to record water meters in turns is no more received by the people. It is an important reform in tap water charge system to introduce "one household one water meter" supported by the people. In this paper, several methods of "one household one water meter" installation and water meter level position installation form in details are introduced.A detailed analysis on its development trends and direction is carried out and it is pointed out that the "one household one water meter" installation will develop toward exposed wall and water meter room type level position installation. Finally, the charge way of IC card (TM Card) type and tele-metering water meter computer metering will be applied. This paper analyzes the hydraulic computation of "one household one water meter" and provides the hydraulic condition of "one household one water meter" installation.     

Application for Hybrid Electric Vehicle Based on CAN Bus

The paper introduces the application of CAN bus in the Hybrid Electric Vehicle, studies the CAN bus technique and the design based on the node of CAN bus, and provides a network topology mode. The theories foundation and basis for new generation automotive are provided, which are combined to introduce the hardware constitutes and software method for the node of CAN bus.     

Predictive Control Approach for Active Power Filter Based on Neural Networks

In this paper, a predictive control approach using neural networks for active power filter is proposed. The control system of active power filter using this method make using of the internal model control technology based on neural networks, meanwhile, to solve its questions such as lag because of calculating using neural networks, a predictive model based on neural networks is introduced. Simulation analysis shows that this control approach can compensate the lag of system and take advantage of self-adaptive characteristic of neural networks. Good result can be obtained.     

八倍体小黑麦与七倍体杂种回交配子间竞争规律的研究

当八倍体小黑麦与七倍体杂种回交时,七倍体×八倍体(以下简称“七×八”)组合的子房受精正常,80％的受精子房不能形成有胚乳的种子,有胚乳种子中能成株的仅有20％,回交效率为1％～2％,远低于八倍体×七倍体(以下简称“八×七”)组合的29.07％。通过分析“八×七”回交种子和七倍体F_1杂种根尖染色体数分布发现,八倍体小黑麦雌配子R染色体平均丢失率为0.133,具28染色体数的整倍体配子对27染色体数的非整倍体配子不具有竞争优势;父本七倍体杂种雄配子R染色体的平均丢失率为0.713,整倍体雄配子的竞争能力是非整倍体(染色体数27)雄配子的48.2倍。“七×八”组合回交效率低的原因可能是雌配子间竞争能力低,各种R染色体数的雌配子都能参与双受精,由于不完整的R组染色体对胚乳发育的干扰,大部分合子不能形成能成株的种子。     

Power Train of a New Type of Hybrid Electric Vehicle

The powertrain of a new type of zero emission electric vehicle is researched, which powered primarily by air-breathing proton exchange membrane (PEM )fuel cells with gaseous hydrogen as fuel,and with Lead-acid battery as assistant power.The fuel cell system provides power for the vehicle while the vehicle travel at a cruising speed,and the exceed power will charge for battery.The fuel cell and the battery system will together provide power to the vehicle during periods of peak power demand such as vehicle acceleration or traveling at a high constant speed.At the same time, the powertrain parameter of this fuel cell vehicle are matched reasonably.At last, the dynamic of an electric vehicle is simulated, and the result shows that the dynamic performance perfectly meets the need of the design target.     

Research on Current Situation and Trend of Control Strategies for Hybrid Electric Vehicles

The vehicle's driving performances is determined by the control strategies and constructions of hybrid electric vehicles (HEV). The constructions of HEV are classified according to the change of state of charge of battery pack, and a study on current situation and trend of control strategies for HEV is presented in this paper. The research indicated that the current control strategies for HEV are not perfect and need further optimization. The control strategies should realize the optimum match among engine, motor, battery and transmission according to the characteristics of each component and vehicle's driving conditions. Many aspects including the fuel economy, emission, the life of battery pack, drivability, the reliability of each component and HEV's manufacture cost should be considered in the control strategies. A study on the control strategies and its optimization that take account of the aspects mentioned above is a work emphasis in the future. This paper also points out the PHEV equipped with small motor, small engine and continuously variable transmission is an ideal system construction for getting optimal fuel economy, lower emissions, steady drivability, lower manufacture cost and HEV's mass.     

One Auto-optimized Fuzzy Control of Car-engines Based on GA

One new auto-optimized fuzzy control model of car-engines based on genetic arithmetic was introduced aiming at the problem that the parameters of car-engine are of the discreteness, the nonlinear and the uncertain characteristics, with which it is difficult to control accurately the excess air coefficient and the spark advance angle in dynamic procedure when using a conventional control model. The rule of the design scheme of the controller achieving the model based on embedded operating system is discussed. Further more, it is probed that the fuzzy control parameters are optimized automatically using GA based on on-line real-time simulation. The results of experiments show that the dynamic indexes and the fuel economy indexes are all improved greatly and the advantage of the model on reducing fuel consumption is obvious.     

小功率管式臭氧发生器电源与定时控制设计

介绍了用于果蔬保鲜的小功率管式电晕放电型臭氧发生器电源电路利用电晕放电原理产生臭氧（O3）的工作过程。根据臭氧发生管放电极的空间布局上的特点,设计双变压器回路给予供电,并辅以STC89C52RC单片机控制电路,实现定时可控功能。该设计克服了传统果蔬保鲜臭氧发生器电源模块庞大的缺点,并根据臭氧发生器的机械结构特点设计了合适的电路板,使电源模块体积减小,节省了安装空间。     

Simulation of The Torque Control System of Motor for Hybrid Vehicle

A strategy for induction motor control based on the grade of errors is developed to improve the performance of hybrid vehicle drive system. The stator flux error, electric magnetic torque error and flux position angle are used as control variables, and rules for the switching states are chosen according to the errors and the principle of the Direct Torquc Control. This strategy improves efficiently the electric magnetic torque response of the system. The result of simulation shows that this strategy is an ideal control strategy of hybrid vehicle, which can make the motor generate the electric magnetic torque efficiently, steadily and quickly.     

Fuzzy Control Strategy and Simulation for ISG-Type Hybrid Electric Vehicles

Fuzzy control strategy is used to study the fuel economy of ISG-Type hybrid electric vehicle with CVT.Based on the instantaneous state of vehicle,the fuzzy logic controller is built aiming to get minimum fuel consumption.In order to avoid the subjective factor,the control rules are established with the optimized results from the sequence quadratic programming(SQP) method.Through the second development of ADVISOR,the simulation for ISG hybrid electric vehicle at different cycles has been accomplished.The results show that the fuzzy logic control strategy is efficient in improving the fuel economy of hybrid electric vehicle.     

Architecture of Networked Manufacturing Based on Supply Chain

The combinationof networked manufacturing and supply chain can integrate the design resource, intelligenceresource and manufacturing resource more efficaciously. Based on the compareand analysisof characteristics of the networked manufacturing and supply chain, the mode of networked manufacturing based on supply chain is brought forward, it combines and integrates supply chain and networked manufacturing to attain the effects of the complementarityof the dominanceof each other, and makes the reality of networked manufacturing more convenience, shortcutand efficiency. Based on the solution, the architecture and an operation mode of networked manufacturing based on supply chain are put forward. Finally, the applicationof networked manufacturing based on supply chain to ceramics industry is studied. Through the implementof the networked manufacturing which is based on the Supply chain dynamic allianceof ceramics industry, the process of from gaining demand information to putting products in the market is finished.     

Hybrid Electric Vehicle and Its Electrical Drive System

Hybrid electric vehicle (HEV) is a mixture of electric vehicle and conventional vehicle, which has the merits of reducing exhaust gas and the consumption of gasoline HEV is the new development way of automobile industry. This paper introduces HEV, and discusses the essential framework of HEV drive system,the basic types of the electrical drive and their features and the present state of the development at home and abroad.Some key technologies are also proposed which determine the drive and control performance of HEV.     

The Cost Calculate Method on Choose Vendors Based on the Supply Chain

At present, when the enterprises that have implemented the SCM chose co - vendors, all kinds of methods have been employed to appraise them. These results are always influenced by the members' subjective attitude at any rate. Based on the fact that the cost is the most important factor when choosing, and according to the requirement of the enterprise on the supply chain, the authors research the cost's component on supply chain. The cost includes: quality cost, price cost, stock cost, OOS cost, transportation cost and other cost. By calculating the products'practical cost synthetically , the enterprises could choose the vendors whose cost is cheapest as their co - vendors. The calculate cost is an objective and feasible method to choosing vendors reasonably. Finally, some examples and related discussion are given.     

An Adaptive Control (AC) Strategy for Internal Grinding with Power Sensor

An adaptive control (AC) strategy for internal grinding with power sensor is proposed.The strategy consists of control strategy of grinding without empty travel,fuzzy control strategy of grinding with constant power,and state monitoring control strategy of grinding wheel.The fuzzy control strategy with constant power is discussed emphatically.     

One-cycle Control Three-phase Four-wire Active Power Filter with Four-arm

This paper presents a three-phase four - wire active power filter based on one-cycle control. This method need not sense the three phase load currents or the three phase voltages, need not use any multipliers. It has simplified circuit , and the controller is composed of four integrators with reset along with several flip-flops, comparators and some analog components. The main circuit switching frequency is constant, which is desirable for industrial applications. Analysis and simulation are carried out with the four-arm inverter, the simulation results verify that the APF with one-cycle control can dynamically and effectually compensate the harmonics, reactive current and zero sequence current.     

Control Method of Single-phase APF

As one-cycle controlled single-phase active power filter(APF) only can compensate harmonics and reactive currents simultaneously,this paper detects circuit based on instantaneous reactive theory and current traces control circuit based on one-cycle control with harmonic current.The control equations and circuit are set up.The results of simulation verify that this scheme is effective and APFs can compensate harmonic and reactive current according to requirement with the distorted source voltages.