杉木、马尾松裸根苗水分关系研究

Water relation parameters of bare-root seedlings of Chinese fir (Cunninghamia lanceolata Hook.)and Masson pine (Pinus massoniana Lamb.)were measured and changes of root growth potential as well as field survival rate of both species were studied after the bare-root seedlings were exposed in a sunny field condition.the results showed that masson pine had a lower osmotic potential(-2.07Mpa) at turgor loss point and at full turgor(-1.29Mpa),compared with Chinese fir(-1.80Mpa and -1.08Mpa respectively).The parameter Vp/Vo(63.27%) of Masson pine was higher than that of chinese fir (58.03%).This means that Masson pine has a stronger ability to tolerate desiccation,compared to Chinese fir according to analysis of above water relation parameters.Root growth potential and field survival rate decreased with prolonging duration of exposure.The field survival rate of both species was reduced to less than 40% after the seedling being exposed only two hours.Water potentials of -1.60 Mpa and -1.70 Mpa were suggested to be critical values for Chinese fir and Masson pine respectively in successful reforestation.     

Improving the assessment method of seed vigor in Cunninghamia lanceolata and Pinus massoniana based on oxygen sensing technology

Oxygen sensing technology was employed to study the rapid methods for seed vigor assessment of Chinese fir (Cunninghamia lanceolata) and Masson pine (Pinus massoniana). Firstly, seeds of five lots were performed using accelerated aging (AA) into three vigor levels. Then, four oxygen sensing indices, including increased metabolism time (IMT), oxygen metabolism rate (OMR), critical oxygen pressure (COP), relative germination time (RGT) and the control indices such as laboratory germination indices, dehydrogenase activity (DA), and electrical conductivity (EC) were analyzed by the tests of 15 samples. The results of correlation analysis between these indices and field emergence performances based on two-year and two-spot data showed that RGT and OMR should be indicated as the optimal oxygen sensing indices to rap- idly and automatically evaluate seed vigor of Chinese fir and Masson pine, respectively. On the basis, one-variable linear regression equations were built to forecast their field emergence performances by the two oxygen sensing indices.     

Research on Change of Rhizosphere Soil Properties of Chinese fir Plantation

This article emphatically reviews the difference of soil biological activities, biochemical activities and soil chemical properties between the rhizosphere and non-rhizosphere soil of first rotation of Chinese fir (Cunninghamia lanceolata (Lamb) Hook) plantation. It also reviews their dynamic patterns during Chinese fir plantation development. The results show that the contents of organic and inorganic nutrients in the rhizosphere soil of young, half-mature and near-mature Chinese fir of first-rotation are more than those in non-rhizosphere soil; except for total K in young Chinese fir plantation. Various patterns of soil nutrients in different stages of Chinese fir plantation development are as follows: available N shows the trend of declination in both rhizosphere soil and non-rhizosphere soil; the content of available P also declines, only increases a little from half-mature stage to near-mature stage, but total P decreases from the beginning to the end. The content of available K increases from youn     

Characterization and Role of Glucose-6-phosphate Dehydrogenase of Populus suaveolens in Induction of Freezing Resistance

Glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) was purified from the leaves of 8-week-old Populus suaveolens cuttings. The enzyme activity in the absence and presence of reduced dithiothreitol (DTTred) was determined. The results show that the G6PDH activity is not inactivated by pre-incubation with DTTred, indicating that the purified enzyme probably presented in cytosol of P suaveolens. The catalytic characteristics and kinetic parameters of cytosolic G6PDH purified from P.suaveolens cuttings were also studied. The results show that G6PDH is characterized by Km value of 360/amol.L^-1 for G6P and 16μmol.L^-1 for NADP, a pH range of 7.3-8.9, and the maximum activity around pH 8.2. The enzyme activity is inhibited by various metabolites such as NADPH, NADH, GTP, UTP, ATP, AMP, ADP, CoA, acetyl CoA, fructose-6-phosphate (F6P), erythrose-4-phosphate (E4P), ribose-5-phosphate (R5P) and 3-phosphoglycerate (3-PG) (all at 1 mmol‘L i except for NADPH and NADH)to different extents. NADPH is the most effective inhibitor of enzyme activity, with an inhibition of 72.0%. The addition of metal ions such as MgC12, CaC12 and KC1 (all 1.0 mmol.L^-1) to the standard reaction mixture has no remarkable influence on the cytosolic G6PDH activity. However, CdCl2 (1.0 mmol.L-1) causes high inhibitory effect on the enzyme activity. To explore the role of G6PDH on the enhancement of freezing resistance induced by freezing acclimation, the changes in the cytosolic G6PDH activity and freezing resistance (expressed as LTs0) of P. suaveolens cuttings during freezing acclimation at -20℃ were investigated. The results reveal that freezing acclimation decreases LTs0 of cuttings, and increases the activity of cytosolic G6PDH compared with control ones,while 2 d of de-acclimation at 25 ~C result in a decrease in cytosolic G6PDH activity, and caused an increase in LT50. Furthermore,the change in cytosolic G6PDH activity is found to be closely correlated to the degree of freezing resistance of cuttings during freezing acclimation. It is suggested that cytosolic G6PDH may be involved in the induction of freezing resistance of cuttings.     

Relations Between Permeability and Structure of Wood

The permeability and the structure of heartwood and sapwood of the solvent-exchange dried and the air-dried green-wood of Chinese-fir(Cunninghamia lanceolata(Lamb.)Hook.)and masson pine(Pinus massoniana Lamb.) were measured in order to study the relations between the permeability and the structure.The results showed that the permeability of sapwood of both the air-dried and the solvent-exchange dried wood was higher than that of heartwood,and the permeability of the solvent-exchanged a bigger number of flow path per unit area of the wood perpendicular to the flow direction resulted from a bigger number of unaspirated pits per unit area and a bigger number of effective pit openings per membrane,and on the other hand,a smaller number of tracheid in series connection per unit length parallel to flow direction resulted from a longer tracheid longth and an effective tracheid length for permeability.     

Preparation and Characterization of Phenolated Wood Using Sulfuric Acid as a Catalyst I: Liquefaction Behavior of Plantation Wood

Chinese fir (Cunninghamia lanceolata) and poplar (Populus spp.) wood meal were phenolated in the presence of sulfuric acid used as a catalyst. The effects of reaction time and reaction temperature on the wood liquefaction were investigated. The results showed that the reaction temperature had the greater influence on the residue content than reaction time. Additionally, the liquefaction curve for the Chinese fir and Poplar were similar in general.     

Investigation of Carbohydrate Compositions for Poplar I-214 and Chinese Fir

Carbohydrates are main compositions for wood (Menachem et al. 1991). The chemical components of poplar I-214 and Chinese fir have been reported (Huang Luohua et al. 2002; Qin Tefu et al. 2003). Through the studies of carbohydrate compositions of poplar I-214 and Chinese fir, different composition of monosaccharides can be found in both poplar I-214 and Chinese fir. 1 MATERIALS AND METHODS 1.1 Materials Wood samples of Chinese fir (Cunninghamia lancedata) and poplar I-214 (Populus…     

Study on Chemical Components of Alkaline Nitrobenzene Oxidation from Plantation Woods

This paper deals with the chemical components and the chemical structure of lignin of Chinese fir and Poplar I-214 from plantationforest. The results revealed that the contents ofchemical component in heart wood and sapwood were almost the same except ethanol-benzene extract both in Chinese fir and in Poplar I-214, The contents of ash, holocellulose, α -cellulose, pentosan, ethanol-benzene extract and cold-water extract in Poplar I-214 were higher than that in Chinesefir, The content of lignin in Poplar I-214 was lower than that in Chinese flr. Vanillin, vanillic acid and p-hydroxybenzaldehyde were found in Chinesefir while vanillin and vanillic acid, p-hydroxybenzoic acid, syringaldehyde and syringic acid werefound in Poplar I-214 in investigation of the chemical structure of lignin by using nitrobenzene oxidization method.     

Sequence-related amplified polymorphism primer screening on Chinese fir(Cunninghamia lanceolata(Lamb.) Hook)

Chinese fir(Cunninghamia lanceolata(Lamb.)Hook) is one of the most important coniferous tree species used for timber production in China. Here, we conducted a sequence-related amplified polymorphism(SRAP) primer screening assay with a total of 594 primer combinations,using 22 forward and 27 reverse primers on four representative Chinese fir genotypes. The obtained results indicated that Chinese fir genomic DNA has a notable amplification bias on the employed forward or reverse primer nucleotides(30selection bases). Out of the tested primer sets, 35 primer combinations with clearly distinguished bands, stable amplification, and rich polymorphism were selected and identified as optimal primer sets. These optimal primer pairs gave a total of 379 scorable bands,including 265 polymorphic bands, with an average of 10.8bands and 7.6 polymorphic bands per primer combination.The produced band number for each optimal primer set ranged from 7 to 14 with a percentage of polymorphic bands spanning from 33.3 to 100.0 %. These primer combinations could facilitate the next SRAP analysis assays in Chinese fir.     

Study on Drying Characteristic of Chinese Fir and Poplar Plantation Wood

The drying characteristic was studied for plantation wood of Chinese fir and poplar, which are typical plantation wood of southern and northern part of China, respectively. Through 100-degree-method the drying characteristic and basic drying condition were gotten, then drying schedule was developed for practical drying, the results showed that the drying schedule is suitable for Chinese fir and poplar plantation lumber, but shrinkage is large. The recommendation was made that enough dead weight is needed to decrease shrinkage in drying process. The drying quality of the two species of lumber is good in conventional drying method.     

Water relation patterns of bare-root and container jack pine and black spruce seedlings planted on boreal cut-over sites

Water relation patterns and subsequent growth were studied on bare-root and container jack pine (Pinus banksiana Lamb.) and black spruce (Picea mariana (Mill.) B.S.P.) seedlings during the first growing season on boreal cut-over sites.Containerized seedlings of both species had greater needle conductance compared to bare-root seedlings over a range of absolute humidity deficits. Needle conductance of containerized seedlings in both species remained high during periods of high absolute humidity deficits and increasing plant moisture stress. Bare-root seedlings of both species had a greater early season resistance to water-flow through the soil-plant-atmosphere continuum (SPAC) than container seedlings. Resistance to water flow through the SPAC decreased in bare-noot seedlings of both species as the growing season progressed, and was comparable to container seedlings 9 through 14 weeks after planting. Four weeks after field planting jack pine container seedlings had greater new root development compared to bare-root seedlings, while at the end of the summer both stock types had similar new root development. Black spruce bare-root seedlings had greater new root development compared with container seedlings throughout the growing season.     

Responses of Pinus massoniana and Pinus taeda to freezing in temperate forests in central China

Abstract

Masson pine (Pinus massoniana Lamb.), a native species widely distributed in temperate forests in central China, and Loblolly pine (Pinus taeda L.), an exotic tree species introduced to China from southeastern United States, are dominant evergreen conifers that play a pivotal role in maintaining forest structure and functions for the region. We examined the effects of freezing on these species with chlorophyll fluorescence and electrolyte leakage using both field- and laboratory-based experiments in September 2009 and January 2010, respectively. We found that freezing could cause a greater impact on the Loblolly pine than the Masson pine. Although the two species showed similar values of F _v /F _m and electrolyte leakage before freezing, the Masson pine needles showed lower F _v /F _m and higher electrolyte leakage ratios than those of the Loblolly pine when treated in low temperatures (?15 to 0°C). We also found that cold-acclimation was crucial for both species to adapt to low temperatures with the F _v /F _m ratio decreased approximately by 80% in the first freezing hour for the non-acclimated needles of both species while the cold-acclimated needles showed little changes in the F _v /F _m ratio. This finding is also supported by our measurements of electrolyte leakage. These results suggest that the Loblolly pine could be more susceptible to freezing damages than the Masson pine in central China.     

马尾松木材材性特点及加工利用研究

马尾松是我国松属树种中地理分布最广的一种,也是南方的造林先锋树种和主要用材树种。文中从物理力学性能、纤维特性以及渗透性等方面论述了马尾松木材材性特点,介绍了马尾松木材干燥及脱脂处理技术、木材蓝变及其防治方法,总结了马尾松木材在实木利用、人造板生产等领域的应用现状,展望了马尾松木材加工利用的发展前景。     

五个主要树种的生物量转换系数分析

利用东北落叶松,南方杉木、马尾松、栎类和西北山杨5个树种(组)的实测数据,对3种平均生物量转换系数计算方法进行了比较分析,并利用假设检验方法,对落叶松和马尾松、2个区域杉木的生物量转换系数进行了差异显著性检验。结果表明:利用算术平均法计算平均生物量转换系数是合适的,阔叶树的生物量转换系数大于针叶树;落叶松和马尾松的生物量转换系数无显著差异,而2个区域杉木的生物量转换系数差异显著。     

马尾松、杉木容器苗培育基质研究

以１０种培养基质，对马尾松和杉木两种树种进行容器育苗研究，结果表明，以竹叶为基本材料配制基质，比纯黄心土基质培育的马尾松和杉木百日苗，干物质重分别增加９３．２％和５３．９％，与蛭石配制的基质相比，分别增加４３．９％和１０４．０％，与松树皮配制的基质相比，分别增加２５．０％和４２．９％，以松针为基本材料配制的基质效果似于竹叶。两者对苗木生长的影响，与各处理相比较，均达到了极显著水平，通过培养基质理化     

水分和热胁迫处理对三个针叶树种苗木叶绿素荧光的影响

本文测定了三个周期水分和热胁迫处理对杉木、马尾松和北美乔柏苗木叶绿素荧光的影响。结果表明：三个树种的可变荧光与最大荧光之比值(Fv／Fm)对水分胁迫的反应不同。水分胁迫处理后北美乔柏的(Fv／Fm)值大大降低，而杉木的(Fv／Fm)值只有轻微下降，马尾松的(Fv／Fm)值则没有显著变化。实验结果还表明：热胁迫处理对三个树种的(Fv，Fm)值都有显著影响。就三个水分和热胁迫周期而言，研究发现：在每个水分和热胁迫周期结束时测定的杉木和马尾松(Fv／Fm)值没有显著差异。但随着胁迫时间的延长，北美乔柏的(Fv／Fm)值显著下降。图4表4参20。     

北亚热带不同森林群落类型的生物多样性研究

北亚热带地区的人工针叶纯林可以通过抚育间伐或者封山育林等措施大大加快其向着该地区的顶级群落方向演替。该地区几种典型群落的乔木层生物多样性指数大小依次为：针阔混交林〉〉常绿阔叶纯林〉马尾松纯林（郁0.5）〉马尾松纯林（CK组）〉杉木纯林（修枝）〉杉木纯林（间伐10%）;灌木层生物多样性指数大小排序为：马尾松纯林（CK组）〉杉木纯林（间伐10%）〉杉木纯林（修枝）〉常绿阔叶纯林〉马尾松纯林（郁0.5）〉针阔混交林。     

In situ experimental freezing produces symptoms of winter injury in red spruce foliage

Two mechanisms have been proposed to explain winter injury to needles of red spruce (Picea rubens Sarg.): (1) desiccation, which is characterized by net loss of foliar water from the needle to the environment, with cell injury resulting from dehydration; and (2) freezing, which is characterized by direct injury to cells resulting from intracellular or extracellular ice formation during exposure to low temperature. To compare the separate and combined effects of freezing and desiccation, branches of a mature red spruce at 1160 m were (a) experimentally frozen in situ to -50 degrees C; (b) cut and tied in their original orientation and allowed to desiccate passively; or (c) both frozen in situ and cut and tied in their original orientation. Needle water content, electrolyte leakage (an index of cell injury), and needle color were monitored for 60 days after treatment. Freezing resulted in immediate increases in electrolyte leakage, rapid water loss, and reddening necrosis of needles similar to that of naturally injured needles. Cutting resulted in more gradual water loss, no significant changes in electrolyte loss until severe desiccation had occurred, and a change in the color of the needles to a dull green. Because freezing produced reddening necrosis, a key symptom of winter injury, whereas desiccation did not, we conclude that freezing is probably the primary cause of winter injury in red spruce, and that desiccation is a secondary effect.