Children Consuming Cassava as a Staple Food are at Risk for Inadequate Zinc, Iron, and Vitamin A Intake

Cassava contains little zinc, iron, and β-carotene, yet it is the primary staple crop of over 250 million Africans. This study used a 24-hour dietary recall to test the hypothesis that among healthy children aged 2–5 years in Nigeria and Kenya, cassava’s contribution to the childrens’ daily diets is inversely related to intakes of zinc, iron, and vitamin A. Dietary and demographic data and anthropometric measurements were collected from 449 Kenyan and 793 Nigerian children. Among Kenyan children 89% derived at least 25% of their dietary energy from cassava, while among the Nigerian children 31% derived at least 25% of energy from cassava. Spearman’s correlation coefficient between the fraction of dietary energy obtained from cassava and vitamin A intake was r = −0.15, P < 0.0001, zinc intake was r = −0.11, P < 0.0001 and iron intake was r = −0.36, P < 0.0001. In Kenya, 59% of children consumed adequate vitamin A, 22% iron, and 31% zinc. In Nigeria, 17% of children had adequate intake of vitamin A, 57% iron, and 41% zinc. Consumption of cassava is a risk factor for inadequate vitamin A, zinc and/or iron intake.     

Combination of On-Line Solid-Phase Extraction with LC-MS for the Determination of Potentially Hazardous Glycoalkaloids in Potato Products

A simple and rapid method for the determination of naturally occurring, potentially hazardous glycoalkaloids (GAs) in potatoes and their products has been developed. The procedure is based on the on-line solid-phase extraction of the acetic acid extracts from potato products and combined with liquid chromatography (LC)-mass spectrometry (MS) in a fully automated system (Symbiosis™, Spark Holland Instruments, Emmen, The Netherlands). As sorbent material HySphere™ 18HD was used for alkaloid enrichment. GAs were eluted with the LC gradient and directly analysed by MS. Detection of the analytes was achieved in the sensitive multiple reaction monitoring mode using two characteristic ions (m/z 98 as a qualifier for GAs and m/z 868.3 as a quantifier for α-solanine or m/z 852.4 for α-chaconine). Typical validation data for method precision (v _k α-solanine = 5.3–6.5, v _k α-chaconine = 3.4–15.4), accuracy (average recovery of α-solanine = 84%, average recovery of α-chaconine = 87%) and linearity over the range from 1 to 1,000 ng ml⁻¹ (R ² = 0.9915 for α-solanine, R ² = 0.9939 for α-chaconine) with detection limits of 0.3 ng ml⁻¹ for α-chaconine and 0.5 ng ml⁻¹ for α-solanine were obtained. GA contents of commercial potato products were determined by the new on-line method and afterwards compared with those obtained with an established high-performance LC routine procedure. Better performance of the on-line procedure was obvious from the standard deviations of both methods. Other advantages included a strong reduction of overall analysis time, human intervention and solvent consumption as well as waste production. The time required for the on-line analysis was 5 min, which would allow processing of almost 100 samples in 8 h.     

Total Phenolics Level,Antioxidant Activities and Cytotoxicity of Young Sprouts of Some Traditional Korean Salad Plants

The aim of this investigation was to study the antioxidant and anticancer activities of young sprouts of some traditional Korean salad plants. Total phenolics, antioxidant and anticancer activities of the methanol extracts from young sprouts of 11 salad plants were determined. The highest amount of phenolics was found in methanol extracts of Euonymus alatus (235.7 mg kg⁻¹), followed by Hypericum ascyron (197.1 mg kg⁻¹), Zanthoxylum piperitum (194.1 mg kg⁻¹) and Zanthoxylum schinifolium (142.5 mg kg⁻¹). Methanol extracts of E. alatus, H. ascyron, and Z. piperitum at 63 mg kg⁻¹ exhibited the highest dose-depend DPPH radical scavenging activity by 91.2, 91.2 and 83.9%, respectively. According to the MTT results, the methanol extracts from Stellaria aquatica, Eleutherococcus sessilifolrus and Z. schinifolium showed the highest anticancer activities against Calu-6 (IC50 < 25.0 μgml⁻¹) and from S. aquatica—the highest anticancer activities against SNU-601 (153.3 μgml⁻¹), following by E. sessilifolrus (196.7 μg ml⁻¹) and Amaranthus mangostanus (303.1 μgml⁻¹). Total phenolics were highly correlated with the DPPH, suggesting that they contribute to the antioxidant properties of the studied plants. In conclusion: young sprouts of Korean salad possess antioxidant and anticancer properties and could be used as a supplement to proper drugs.     

Critical water content and water stress coefficient of soybean (Glycine max [L.] Merr.) under deficit irrigation

The objective of this research was to investigate the critical water content (θ _c) and water stress coefficient (K _s) of soybean plant under deficit irrigation. This research was conducted in a plastic house at the University of Lampung, Sumatra in Indonesia from June to September 2000. The water deficit levels were 0–20%, 20–40%, 40–60%, 60–80%, and 80–100% of available water (AW) deficit, arranged in Randomized Completely Block (RCB) design with four replications. The results showed that the soybean plant started to experience stress from week IV within 40–60% of AW deficit. The fraction of total available water (TAW) that the crop can extract from the root zone without suffering water stress (p) was 0.5 and θ_c was 0.305 m³ m⁻³. The values of K _s at p=0.5 were 0.78, 0.86, 0.78, and 0.71 from week IV to week VII, respectively. The optimum yield of soybean plant with the highest yield efficiency was reached at 40–60% of AW deficit with an average K _s value of 0.78; this level of deficit irrigation could conserve about 10% of the irrigation. The optimum yield of soybean plant was 7.9 g/pot and crop water requirement was 372 mm.     

Versuche zur Übertragung der Kartoffelviren M und S DurchMyzus Persicae Sulz

Zusammenfassung In übertragungsversuchen mitMyzus persicae konnten die folgenden Isolate des M-Virus von Pflanzen der gegen das S-Virus immunen KartoffelsorteSaco aufSolanum demissum als Testpflanze übertragen werden: “leaf rolling mosaic”, “interveinal mosaic”, “Fortuna” und “D 1102”. Nicht übertragbar unter den gew?hlten Bedingungen (48–72 Std. Saugzeit) war das “Paracrinkle”-Isolat. Es stimmt hierin mit dem S-Virus aus demUS-S?mling 41956 überein. Die Bedeutung der übertragungsverh?ltnisse für die Klassifizierung der Kartoffelviren M und S sowie des serologisch verwandten, blattlausübertragbaren “carnation latent-Virus” wird diskutiert.

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Summary In transmission experiments withMyzus persicae the following isolates of virus M were found to be transmittable toSolanum demissum test plants from potato plants of the varietySaco, which is immune to virus S: leaf rolling mosaic, interveinal mosaic, Fortuna and D 1102. The paracrinkle isolate was not transmittable under the conditions chosen (48–72 hours sucking time). In this respect it corresponds to the virus S from theU.S. Seedling 41956. The importance of transmission conditions for the classification of potato viruses M and S as well as of the serologically related and aphid-transmittable carnation latent virus is discussed.

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Résumé Dans les essais de transmission parMyzus persicae, les isolés suivants du virus M, obtenus de la variété de pomme de terreSaco, immune au virus S, ont pu être transmis sur les plantes test deSolanum demissum: “leaf rolling mosaic”, “interveinal mosaic”, “Fortuna” et “D 1102”, L'isolé du “paracrinkle” n'était pas transmissible dans les conditions choisies (durée de pompage 48 à 72 heures). Sous ce rapport, il ressemble au virus S duUS-S?mling 41956. L'importance des conditions de transmission pour la classification des virus M et S de la pomme de terre ainsi que pour celle du “carnation latent-virus” sérologiquement apparenté et transmissible par le puceron est discutée.

     

Carotenoid Composition of Jackfruit (<Emphasis Type="Italic">Artocarpus heterophyllus</Emphasis>), Determined by HPLC-PDA-MS/MS

Carotenoids are pigments responsible for the yellow-reddish color of many foods and are related to important functions and physiological actions, preventing several chronic-degenerative diseases. The objective of this study was to confirm the carotenoid composition of jackfruit by high-performance liquid chromatography connected to photodiode array and mass spectrometry detectors (HPLC-PDA-MS/MS). The main carotenoids were all-trans-lutein (24–44%), all-trans-β-carotene (24–30%), all-trans-neoxanthin (4–19%), 9-cis-neoxanthin (4–9%) and 9-cis-violaxanthin (4–10%). Either qualitative or quantitative differences, mainly related to the lutein proportion, were found among three batches of jackfruit. Since the fruits from batch A showed significantly lower contents for almost all carotenoids, it also had the lowest total carotenoid content (34.1 μg/100 g) and provitamin A value, whereas the total carotenoid ranged from 129.0 to 150.3 μg/100 g in the other batches. The provitamin A values from batches B and C were 3.3 and 4.3 μg RAE/100 g, respectively. The carotenoid composition of jackfruit was successfully determined, where 14 of the 18 identified carotenoids were reported for first time. Differences among batches may be due to genetic and/or agricultural factors.     

Circulating Salicylic Acid and Metabolic and Inflammatory Responses after Fruit Ingestion

We hypothesized that fruit ingestion provides measurable amounts of salicylic acid (SA) and produces different metabolic and inflammatory responses compared to mere fruit sugars. In a randomized-crossover study, 26 healthy subjects received a peach shake meal (PSM) (SA: 0,06 ± 0,001 mg/100 g) and a mixed sugar meal (MSM), consisting in an aqueous solution with the same sugars found in the peach shake. In order to control for the SA contribution from meals in the previous day, 16 subjects (Group 1) abstained from fruits and vegetables consumption the evening before trials, and 10 subjects (Group 2) maintained their usual diet. Circulating SA, glucose, insulin, free fatty acids, and interleukin-6 were determined. Basal SA was lower in Group 1 than in Group 2 (0.09 ± 0.02 vs. 0.30 ± 0.03 μmol/l, p < 0.001), peaked at 90 min in both groups (0.18 ± 0.01 vs. 0.38 ± 0.02 μmol/l, p < 0.01) and remained above baseline (p < 0.05) up to 3 h. Glycemia increased less after PSM at 15 min (p < 0.01) with a lower average glucose excursion (p < 0.05). Insulin peaked at 45 min with both meals but decreased less rapidly with PSM. Free fatty acids decreased more (p < 0.01), and interleukin-6 increased less (p < 0.05) with PSM. Dietary fruit intake increases the concentration of SA in vivo, and provides non-nutrients capable to modulate the inflammatory and metabolic responses to carbohydrates.     

Influence of storage temperature on rate of potato tuber tissue infection caused byPhytophthora infestans (mont.) de bary estimated by digital image analysis

Summary Potato tubers were inoculated with two biotypes ofPhytophthora infestans then stored at 3,7, 10 and 15°C. Image analysis quantified average reflective intensity (ARI) of diseased tissue from cut surfaces of sample tubers. Tuber tissue infection and infection rate were measured by calculating Mean ARI of samples. Average tuber tissue infection and infection rate was minimal at 3°C (P.i.-US8 orP.i.-US1). Tuber tissue infection increased at temperatures >3°C, from 220 Mean ARI seven days after inoculation (dai) to 190–150 Mean ARI 50 dai (depending on cultivar and biotype ofP. infestans). Rate of tuber tissue infection caused byP.i.-US1 at 7°C was about zero in cv. Snowden but greater than −0.2 ARI day⁻¹ (cvs Russet Burbank and Superior). Rate of late blight infection in tuber tissue generally increased with temperature from −0.2 ARI day⁻¹ (at 7°C) in all cultivars to a maximum of −0.8 ARI day⁻¹ (10°C).     

Structure development and dynamic properties in high-speed spinning of high molecular weight PEN/PET copolyester fibers

The structure development and dynamic properties of fibers produced by high-speed spinning of P(EN-ET) random copolymers were investigated. The as-spun fibers were found to remain amorphous up to the spinning speed of 1500 m/min, and subsequent increases in speed resulted in the crystalline domains containing primarilyα crystalline modification of PEN. Theβ modification was not found up to spinning speeds of 4500 m/min. On the other hand, annealing of constrained fibers spun at the 2100 m/min at 180, 200, and 240°C exhibitedβ-form crystalline structure, while the annealed fibers spun in 600–1500 m/min range exhibited dominantlyα-form. Howeverβ-form crystals disappeared above the spinning speed of 3000 m/min. With increasing spinning speeds from 600 to 4500 m/min, the storage modulus of as-spun fibers increased continuously and reached a value of about 10.4 Gpa at room temperature. The tanδ curves showed theα-relaxation peak at about 155–165°C, which is considered to correspond to the glass transition. Theα-relaxation peaks became smaller and broader, and shift to higher temperatures as the spinning speed increases, meaning that molecular mobility in the amorphous region is restricted by increased crystalline domain.     

Synthesis of thermotropic polyurethanes containing imide units and their mesophase behavior

Thermotropic polyurethanes were synthesized from 1,6-hexane diisocyanate (HDI) as a diisocyanate, 1,6-hexane diol (HD), and rigid diols containing imide unit such as N,N′-bis(4-hydroxyphenyl)-3,4,3′,4′-biphenyl-dicarboxyimide (BPDI) or bis-N-(4-hydroxyphenyl)-4,4′-oxydiphthalimide (ODPI). The effects of structure difference between BPDI and ODPI and composition of HD/BPDI (ODPI) on the thermal and liquid crystalline behavior were studied. Thermotropic polyurethanes with an inherent viscosity of 0.59–0.70 were obtained. The melting temperature of BPDI-based polyurethanes were in the range of 150–290°C, however, those of ODPI-based polyurethanes were in the range of 150–190°C. All the polyurethanes based on ODPI (25–100 mole %) clearly exhibited a stable liquid crystalline phase, and BPDI-based polyurethane having 5–25% of BPDI showed a mesophase. The melting and isotropization temperatures (T _m , T _i ) andΔT(T _i −T _m ) increased with increasing BPDI and ODPI content. The polyurethanes based on BPDI has higher melting points and thermal stability compared to ODPI-based polyurethanes.     

Chemical and structural characterization of lignins isolated from <Emphasis Type="Italic">Caragana sinica</Emphasis>

Successive extractions of the dewaxed Caragana sinica with 70 % ethanol, 70 % ethanol containing 1 % NaOH, 1 M KOH, 1 M NaOH, 3 M KOH, and 3 M NaOH at 75 °C for 3 h released 80.4 % of the original lignin. The physico-chemical properties and structural features of these lignin fractions were comprehensively characterized by alkaline nitrobenzene oxidation, GPC and two-dimensional NMR spectroscopy. The results showed that all the lignin fractions had relatively lower molecular average-weights ranging between 910 and 1630 g/mol. Moreover, 2D-NMR showed that the lignin fraction L₂ isolated with 70 % ethanol containing 1 % NaOH from C. sinica was similar to a hardwood lignin, with a high S/G ratio accounted for 1.2, a predominance of β-O-4′ aryl ether linkages (77 % of all side-chains), followed by β-β′ resinol-type linkages (8 %) and lower amounts of β-5′ phenylcoumaran substructures (7 %), β-1′ spirodienone-type linkages (3 %), and cinnamyl end groups (5 %). The high predominance of the S-lignin units, together with the high proportion of β-O-4′ aryl ether linkages could contribute to a relatively high reactivity of C. sinica lignin during alkaline pulping.     

Identification and Quantification of Steroidal Alkaloids from Wild Tuber-Bearing <Emphasis Type="Italic">Solanum</Emphasis> Species by HPLC and LC-ESI-MS

Wild Solanum species are characterized by several types of glycoalkaloids (GAs), which are usually not found in commercial potato cultivars. These alkaloids serve as defence compounds against herbivores and microorganisms, especially fungi. In this study, the GA composition of tuber and leaf material of 17 wild Solanum species was analysed qualitatively by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) and quantitatively by high-performance liquid chromatography. The GAs identified were α-solanine, α-chaconine, β-chaconine, solasonine, solamargine, demissine, dehydrodemissine, α-tomatine, dehydrotomatine, commersonine, dehydrocommersonine, leptine I and leptine II. Most species accumulated the common potato GAs α-solanine and α-chaconine in tubers and solasonine and solamargine additionally in leaves. In some species, such as S. acaule ssp. acaule, S. demissum and S. polyadenium, substantial amounts of unusual alkaloids were detected. By using LC-ESI-MS, we detected several minor alkaloids such as dehydrogenated forms of α-tomatine, demissine and commersonine for the first time. Total GA content, expressed as the sum of the four main alkaloids α-solanine, α-chaconine, solamargine and solasonine differed from species to species. In general, GA contents in leaf tissue were higher and GA patterns were more complex than those of tubers.     

High Mr Glutenin Subunits of Indian Wheat Cultivars: Association of Subunits 5 + 10 with the 1BL/1RS Wheat-Rye Translocation

The seed proteins of 110 commercially-released Indian wheat cultivars were fractionated using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to determine their high M_r glutenin subunit compositions. Amongst the cultivars, three alleles were indentified for the Glu-A1 locus (subunits 1, 2^* and the null phenotype), eight alleles for the Glu-B1 locus (7, 7 + 8, 7 + 9, 6 + 8, 20, 13 + 16, 17 + 18 and a new allele) and two for the Glu-D1 locus (2 + 12 and 5 + 10). Nine of the cultivars were heterogeneous and possessed two or more 'biotypes' with respect to high M_r subunits. The cultivars were also analysed for the presence of the 1BL/1RS wheat-rye translocation by SDS-PAGE of unreduced prolamins and hybridisation of DNA dot blots with a rye-telomere-specific repetitive DNA probe, pAW-161. Both methods revealed that the majority of newly-released Indian wheat cultivars carry this translocation, thus confirming the agronomic superiority of these lines. While most of the normal wheat cultivars possessed high M_r subunits 2 + 12, 14 of the 18 translocation cultivars had the allelic subunits 5 + 10, even though no selection was made for these subunits during the breeding process. This suggests that the subunits 5 + 10 may play a compensating role for the loss of dough strength associated with the 1BL/1RS translocation.     

Sound characteristics according to cross-sectional shapes of fibers

In order to investigate the effects of cross-sectional shapes on the sound characteristics of polyester fibers, 10 specimens were woven into a twill structure made of round, hollow, triangular, u-shape, cruciform, and composite cross-sectional (▴/▴, ()/▴,Y y) fibers. Their rustling sounds were recorded, and their sound spectra were obtained from FFT analysis. Physical sound parameters (LPT, ΔL, Δf) and Zwicker’s psychoacoustic parameters of the loudness(Z), sharpness(Z), roughness(Z), and fluctuation strength(Z) were calculated from the sound spectra. According to noncircular cross-section fibers, the hollow shaped fiber had the highest value of LPT, ΔL, loudness(Z), and fluctuation strength(Z). The triangular shaped fiber had a lower value of LPT, ΔL, loudness(Z), and roughness(Z) than those of the round shaped fiber. Among composite cross-section fibers, C1 (▴/▴) and C3 (Y y) had higher values of LPT, ΔL, Δf, and loudness(Z) but C2 (()/▴) had lower values. Also the LPT, ΔL, sharpness(Z), and roughness(Z) values of different denier were similar to each other, but the Δf and loudness(Z) values increased as the denier increased.     

Modeling the tensile strains of non-uniform fibers

The maximum strain experienced by the thinnest segment of a non-uniform fiber governs fiber breakage, yet this maximum strain can not be obtained from a normal single fiber test. Only the average strain of the whole fiber specimen can be obtained from a normal single fiber tensile test. This study has examined the relationship between the average strain, the maximum strain and the degree of fiber non-uniformity, expressed in coefficient of variation (CV) of fiber diameters along fiber length. The tensile strain of irregular fibers has been simulated using the finite element method (FEM). Using this method, average and maximum tensile strains of non-uniform fibers were calculated. The results indicate that for irregular fibers such as wool, there is an exponential relationship (i.e.ɛ _ave ɛ _max=ae ^{−b CV} ) between the ratio of average breaking strain and maximum breaking strain (ɛ _ave ɛ _max) and the along-fiber diameter variation (CV). The strain ratio decreases with the increase of the along-fiber diameter variation.     

Volatile Compounds and Antioxidative Activity of <Emphasis Type="Italic">Porophyllum tagetoides</Emphasis> Extracts

Porophyllum tagetoides is an annual warm-weather herb that has an intense typical smell. Its leaves are commonly used in soup preparation and traditional medicine for treatment of inflammatory diseases. Its volatile compounds and antioxidant properties were evaluated in crude, aqueous and ethanol leaf extract and an oil emulsion using different antioxidant assays in vitro, such as: DPPH radical scavenging activity, redox potential, polyphenol content, reducing power and optical density. A high antioxidative activity was found when comparing leaves with stems. The crude extract from leaves showed a very high reducing power (2.88 ± 0.20 O.D.) and DPPH radical-scavenging activity (54.63 ± 4.80%), in concordance with a major concentration of vitamin C (23.97 ± 0.36 mg/100 g). Instead, the highest polyphenol content (264.54 ± 2.17 mg GAE/g of sample) and redox potential (561.23 ± 0.15 mV) were found by the ethanol and aqueous extract, respectively. Aldehydes and terpenes such as nonanal, decanal, trans-pineno, β-myrcene and D-limonene were the major volatiles found. This study suggests that Porophyllum tagetoides extracts could be used as antioxidants.     

Phytosterol,Squalene, Tocopherol Content and Fatty Acid Profile of Selected Seeds,Grains, and Legumes

The unsaponifiable lipid fraction of plant-based foods is a potential source of bioactive components such as phytosterols, squalene, and tocopherols. The objective of the present study was to determine the levels of phytosterols, and squalene, as well as tocopherols (α and β + γ) in selected grains, seeds, and legumes. The method comprised acid hydrolysis and lipid extraction followed by alkaline saponification, prior to analysis by HPLC. In addition, the fatty acid profile of the foods was determined via total lipid extraction, fatty acid derivitisation and GC analysis. In general, β-sitosterol was the most prevalent phytosterol, ranging in concentration from 24.9 mg/100 g in pumpkin seed to 191.4 mg/100 g in peas. Squalene identified in all foods examined in this study, was particularly abundant in pumpkin seed (89.0 mg/100 g). The sum of α- and β+ γ-tocopherols ranged from 0.1 mg/100 g in rye to 15.9 mg/100 g in pumpkin seeds. Total oil content ranged from 0.9% (w/w) in butter beans to 42.3% (w/w) in pumpkin seed and the type of fat, in all foods examined, was predominantly unsaturated. In conclusion, seeds, grains, and legumes are a rich natural source of phytosterols. Additionally, they contain noticeable amounts of squalene and tocopherols, and in general, their fatty acid profile is favorable.     

Preparation and dielectric properties of polyvinyl alcohol (Co,Zn Acetate) Fiber/n-Si and polyvinyl alcohol (Ni,Zn Acetate)/n-Si Schottky diodes

Dielectric properties and ac electrical conductivity (σ _ac ) of Au/PVA(Co, Zn acetate)/n-Si and Au/PVA(Ni, Zn acetate)/n-Si Schottky diodes (SDs) have been investigated in dark and under illumination by using experimental capacitance-voltage (C-V) and conductance-voltage (G/ω-V) measurements at 1 MHz and room temperature. Experimental results indicate that the change in dielectric constant (ɛ′), dielectric loss (ɛ″), loss tangent (tanδ), the real (M′) and imaginary (M″) parts of electric modulus and ac electrical conductivity (σ _ac ) with illumination were found to change linearly with illumination level (P). On the other hand, the ɛ′, ɛ″, tanδ and σ _ac vs P have positive slope while the M′ and M″ vs P have negative slope. Such behavior of dielectric properties and σ _ac can be attributed to illumination induced electron-hole pairs under illumination effect in the depletion region of SDs. The obtained results under illumination suggest that these devices can be used as a sensor in optical applications.     

Application of acacia natural dyes on cotton by pad dyeing

Coloration of textiles, traditionally achieved using natural dyes, commonly employs synthetic dyes at the industrial level. A revival of commercial interest in natural dyes has opened several research avenues. This paper investigates the application by padding of cotton fabric with 10 g/l of two natural dyes derived from the Acacia plant family. Three mordanting techniques were studied; of which post-mordanting produced the most even shade. Among the two mordants investigated, the use of copper sulfate resulted in a level beige shade at 15 g/l concentration while ferrous sulfate performed best at 5 g/l yielding a yellow-grey shade. An optimum process-sequence for the copper sulfate mordant was “pad (dye)→dry→steam followed by pad (mordant)→steam→dry”, and for ferrous sulfte it was “pad (dye)→steam→dry followed by pad (mordant)→steam→dry”. Typically a change in mordant resulted in a different shade with the same dye. The study concluded that padding is a readily adaptable process for the dyeing of cotton using natural dyes and acceptable fastness in shades can be obtained.     

Amylolytic activity in stored potato tubers. 2. The effect of low-temperature storage on the activities of α-and β-amylase and α-glucosidase in potato tubers

Summary Tubers of the potato cultivars Record, Wilja, Pentland Dell and Brodick (formerly clone 137371) were sampled before and after storage at either 4°C or 10°C. Reducing sugar content stayed constant during storage at 10°C in all four cultivars but rose greatly during the first 6–12 weeks of storage at 4°C in Record, Wilja and Pentland Dell but not in Brodick. Amylolytic activity was determined after 5 weeks storage using blockedp-nitrophenyl maltoheptaoside as substrate for α-amylase,p-nitrophenyl maltopentaoside as substrate for β-amylase, andp-nitrophenylglucopyranoside as substrate for α-glucosidase. The values obtained from tubers stored at 4°C were higher than those from tubers stored at 10°C, the differences being much less in Brodick than in the other three cultivars.