烘干橄榄叶粉对蛋鸡生产性能、蛋品质及抗氧化的影响

文章旨在探讨日粮添加不同水平的橄榄叶粉对蛋鸡产蛋性能、蛋品质和血清抗氧化功能的影响。试验将产蛋性能一致的576只24周龄海兰褐壳蛋鸡随机分为4组，每组4个重复，每个重复36只。对照组饲喂基础日粮，T1～T3组分别饲喂基础日粮+5、10和20?g/kg橄榄叶粉，试验进行8周。结果：与对照组相比，日粮添加5、10和20?g/kg橄榄叶粉使蛋鸡的末重分别显著提高1.97%、1.53%和1.86%（P＜0.05），而平均日增重也分别显著提高36.90%、30.89%和36.85%（P＜0.05）。10和20?g/kg橄榄叶粉组鸡蛋的蛋黄颜色较对照组分别显著提高了37.79%和41.56%（P＜0.05），而5?g/kg橄榄叶粉组鸡蛋的哈夫单位较对照组显著提高5.85%（P＜0.05）。对照组蛋鸡血清胆固醇含量较处理组分别显著提高26.68%、32.47%和42.08%（P＜0.05），而对照组蛋鸡血清甘油三酯浓度较20?g/kg橄榄叶粉组显著提高41.71%（P＜0.05）。与对照组相比，5、10和20?g/kg橄榄叶粉组蛋鸡血清谷胱甘肽过氧化物酶活性分别显著提高（P＜0.05）。结论：在本研究条件下，日粮橄榄叶粉渣添加水平达到20?g/kg时对蛋鸡的产蛋性能无负面影响，补充水平达到5?g/kg橄榄叶粉渣可以提高鸡蛋的哈夫单位和蛋黄颜色，降低血清胆固醇，改善血清抗氧化功能。 [关键词]橄榄叶粉|蛋鸡|生产性能|蛋品质|抗氧化     

妊娠后期和泌乳期日粮添加生姜、八角和丹参对母猪抗氧化性能和繁殖性能的影响

试验旨在研究妊娠后期和泌乳期日粮中添加生姜、八角和丹参对母猪抗氧化性能和繁殖性能的影响。试验选择胎次一致、平均体重(164.28±1.30) kg的莱芜黑猪妊娠经产母猪36头,随机分为4个处理,每个处理9个重复。对照组饲喂基础日粮,试验组在对照组的基础上分别添加10g/kg姜粉、5g/kg八角粉和10g/kg丹参粉(即Cont.组、GIN组、SA组和DS组)。结果表明,GIN组、SA组和DS组母猪血清中GSH含量、初乳中乳蛋白含量及初乳T-AOC均显著高于Cont.组(P<0.05);GIN组和DS组初乳中乳脂含量均显著高于Cont.组(P<0.05),SA组和DS组初乳中乳糖含量显著高于Cont.组(P<0.05);各处理间窝产活仔数、21 d成活率、仔猪初生窝重和21 d窝重均无显著差异(P>0.05)。综上所述,日粮中添加10g/kg生姜、5g/kg八角和10g/kg丹参可提高母猪血清和初乳的抗氧化水平,提高初乳营养价值,但对母猪繁殖性能无影响。     

酵母提取物对白对虾生长性能、抗氧化及机体成分的影响

文章旨在评估日粮添加不同水平的酵母提取物对白对虾生长性能、抗氧化及机体成分的影响。试验将800条平均初始体重为1.35?g的白对虾随机分为4组,每组4个重复,每个重复50条,对照组饲喂基础日粮,T1～T3组在基础日粮中添加10、15和20?g/kg酵母提取物（添加量用等量的基础日粮中的面粉替换）,试验共开展8周。结果：与对照组相比,20?g/kg酵母提取物组虾的末重和特定生长率分别显著提高5.33%和2.58%（P＜0.05）。20?g/kg酵母提取物组虾的相对增重较其他组分别显著提6.02%、4.92%和5.29%（P＜0.05）。对照组虾的料重比较15?g/kg酵母提取物组显著提高10.23%（P＜0.05）,同时对照组虾的外型指数较酵母提取物组分别显著降低6.25%、4.76%和6.25%（P＜0.05）。20?g/kg酵母提取物组虾血清谷胱甘肽过氧化物酶活性最高（P＜0.05）,而对照组虾血清丙二醛含量最高（P＜0.05）。与对照组相比,15和20?g/kg酵母提取物组虾血清总抗氧化力活性分别显著提高29.51%和32.17%（P＜0.05）。与对照组相比,10和20?g/kg酵母提取物组白对虾机体粗脂肪含量分别显著提高21.24%和20.0%（P＜0.05）。对照组白对虾机体水分含量较酵母提取物分别显著提高3.67%、4.16%和4.77%（P＜0.05）。结论：在本试验条件下,日粮添加酵母提取物可以改善白对虾的血清抗氧化力,当添加水平达到15?g/kg时可提高白对虾的饲料效率。 [关键词]酵母提取物|白对虾|生长性能|抗氧化|机体成分     

饲粮中添加辣木梗粉对蛋雏鸭生长、免疫及抗氧化功能的影响

本试验通过在饲粮中添加不同水平的辣木梗粉,研究其对1～28日龄蛋雏鸭生长、免疫及抗氧化功能的影响,以探讨辣木梗粉的适宜添加量。试验选取健康、体重相近的1日龄三穗蛋雏鸭135只,随机分为3组(Ⅰ～Ⅲ组),每组3个重复,每个重复15只试鸭。采用基础饲粮加辣木梗粉进行饲喂,Ⅰ组(对照组)饲喂基础饲粮,Ⅱ、Ⅲ组在基础饲粮中添加20 g/kg和40 g/kg辣木梗粉,试验期4周。结果表明:20 g/kg辣木梗粉添加组的平均日采食量(ADFI)分别比空白对照组和40 g/kg辣木梗粉添加组显著提高8.6%和9.8%(P <0.05);20 g/kg和40 g/kg g辣木梗粉添加组的平均日增重(ADG)分别比空白对照组显著提高11.8%和18.3%(P <0.05);40 g/kg辣木梗粉添加组的料重比(F/G)分别比空白对照组和20 g/kg辣木梗粉添加组显著降低16.2%和14.1%(P <0.05);20 g/kg和40 g/kg辣木梗粉添加组血清白蛋白(ALB)含量比对照组分别显著提高39.1%和49.5%(P <0.05);20 g/kg辣木梗粉添加组的免疫蛋白M(IgM)含量比空白对照组显著提高了1.6%(P <0.05);20 g/kg辣木梗粉添加组的血清丙二醛(MDA)含量比空白对照组显著减少33.16%(P <0.05)。综上,在基础饲粮中添加适量的辣木梗粉可提高1～28日龄蛋雏鸭的免疫及抗氧化功能,对辣木梗粉在蛋雏鸭的应用上提供了参考依据。     

饲粮维生素E水平对鹅免疫和抗氧化功能的影响

本试验旨在研究饲粮维生素E水平对鹅免疫和抗氧化功能的影响,以确定1～12周龄鹅饲粮维生素E的最适添加量。选用1日龄平均体重为(111.1±6.5)g青农灰鹅180只,随机分为6组,每组3个重复,每个重复10只。6组试验鹅分别饲喂在基础饲粮中添加0(对照组)、5、10、20、40和80 IU/kg维生素E的试验饲粮。试验期为12周。结果表明:1)与对照组相比,10、20、40、80 IU/kg维生素E组4周龄和12周龄胸腺指数显著或极显著增加(P<0.05或P<0.01),20、40、80 IU/kg维生素E组4周龄和12周龄法氏囊指数显著增加(P<0.05)。饲粮添加20 IU/kg维生素E可显著或极显著提高新城疫免疫后7、14、21 d的抗体滴度(P<0.05或P<0.01),添加20、40、80 IU/kg维生素E可显著或极显著提高4周龄和12周龄的外周血淋巴细胞转化率(P<0.05或P<0.01)。2)血清和肝脏谷胱甘肽过氧化物酶(GSH-Px)活性,4周龄时20、40、80 IU/kg维生素E组显著高于对照组(P<0.05);12周龄时10、20、40、80 IU/kg维生素E组显著高于对照组(P<0.05)。与对照组相比,4周龄时40 IU/kg维生素E组血清总超氧化物歧化酶(T-SOD)活性显著升高(P<0.05);4周龄时10 IU/kg维生素E组肝脏T-SOD活性显著升高(P<0.05);12周龄时20、40、80 IU/kg维生素E组肝脏T-SOD活性显著升高(P<0.05)。4周龄时40 IU/kg维生素E组,12周龄时20、40、80 IU/kg维生素E组血清总抗氧化能力(T-AOC)显著高于对照组(P<0.05);4周龄时20 IU/kg维生素E组肝脏T-AOC显著高于对照组(P<0.05)。3)与对照组相比,4周龄和12周龄时各维生素E添加组血清和肝脏丙二醛(MDA)含量均有不同程度地降低;4周龄和12周龄时40 IU/kg维生素E组血清过氧化氢酶(CAT)活性显著升高(P<0.05)。由此得出:1)饲粮中添加维生素E可提高鹅的免疫和抗氧化功能。2)运用回归方程预测,最佳免疫和抗氧化条件下1～12周龄鹅饲粮维生素E适宜添加量为44.31～53.11 IU/kg;以血清MDA含量为参考依据确定1～12周龄鹅饲粮维生素E最低添加量为6.84 IU/kg。     

生姜粉在饲料储藏和畜禽生产中应用研究进展

研究生姜粉在饲料储藏和畜禽生产中应用。将生姜(ginger)风干后40目粉碎,以不同比例添加到含VA(4 500 IU/kg)、VD3(1 250 IU/kg)、VE(12.5 IU/kg)和VB2(3.0 mg/kg)的玉米粉和大豆粉,以及蛋鸡和生长育肥猪配合饲料中,研究生姜粉添加对饲料脂质氧化和维生素保护的功效。同时,系统研究了在商品肉鸡、蛋鸡、繁殖母猪、生长育肥猪、肉牛和肉羊日粮中添加不同梯度生姜粉对畜禽生产性能和机体抗氧化的作用。结果表明:(1)生姜粉可以作为饲料抗氧化剂,减少饲料贮藏过程中脂质氧化和维生素的破坏。1.0%生姜粉可以保护VA、VD3、VE和VB2稳定性(P0.05)。(2)生姜粉可用作改善畜禽生产性能和饲料效率。日粮添加1.0%生姜粉可以改善肉鸡、产蛋鸡、母猪和仔猪的生长性能。牛日粮使用1.0 g/kg生姜粉有利于瘤胃VFA发酵和蛋白质合成,羊日粮使用0.5 g/kg的生姜粉增加丙酸产量(P0.05)。(3)生姜粉可用作动物机体及其产品抗氧化能力提升。日粮添加0.5%的生姜粉可以改善肉鸡抗氧化和产肉性能,而1.5%生姜粉才能改善蛋鸡抗氧化和蛋品质。母猪、生长育肥猪日粮添加1.0%生姜粉可以改善机体和猪肉抗氧化功能。肉牛日粮添加1.5 g/kg生姜粉有利于血液抗氧化指标改善(P0.05)。综上结论:1.0%生姜粉可以作为饲料抗氧化剂,改善饲料的氧化稳定性,保护维生素,改善肉鸡、蛋鸡、母猪和生长育肥猪的生长性能。肉鸡0.5%、猪1.0%和蛋鸡1.5%日粮生姜粉添加能改善机体抗氧化能力。肉牛日粮添加生姜粉能改善机体抗氧化能力(1.5 g/kg)和饲料利用率(1.0 g/kg)。     

日粮添加海藻粉和抗菌肽对海兰褐蛋鸡免疫功能的影响

以24周龄海兰褐蛋鸡为研究对象,采用4×2(海藻粉×抗菌肽)完全随机设计,研究日粮添加不同水平的海藻粉(0、1%、3%和5%)和抗菌肽(300和600 mg/kg)对蛋鸡免疫功能的影响。结果表明:在试验第31周末,海藻粉提高蛋鸡血清中禽流感抗体效价及全血中粒细胞(P0.05);抗菌肽提高全血中粒细胞(P0.05);海藻粉和抗菌肽互作提高蛋鸡血清中禽流感抗体效价及全血中粒细胞(P0.05)。说明:海藻粉和抗菌肽对蛋鸡免疫功能的影响存在协同效应,以海藻粉5%及抗菌肽300 mg/kg水平组合最为理想。     

低锌饲粮添加植酸酶对1～4周龄鹅生长性能、胫骨发育、免疫性能及抗氧化能力的影响

本试验旨在研究低锌饲粮添加植酸酶对1~4周龄鹅生长性能、胫骨发育、免疫性能及抗氧化能力的影响,探索饲粮添加植酸酶能否提高锌生物学效价和有效降低锌的添加水平。选用1日龄五龙鹅360只,随机分为6组,每组6个重复,每个重复10只鹅(公母各占1/2)。Ⅰ组为正对照组,饲喂基础饲粮,添加80 mg/kg硫酸锌,未添加植酸酶;Ⅱ组为负对照组,饲喂基础饲粮,添加1 200 U/kg植酸酶,未添加锌;Ⅲ~Ⅵ组在负对照组的基础上分别添加16、32、48、64 mg/kg硫酸锌。试验期28 d。结果表明:1)通过二次曲线拟合分析,在饲粮添加1 200 U/kg植酸酶的条件下,饲粮锌添加水平为34.00~44.70 mg/kg时可获得最佳平均日增重与料重比。2)Ⅳ、Ⅴ组的骨密度(BMD)显著高于Ⅱ组(P0.05);Ⅳ、Ⅴ组的骨矿含量(BMC)和血清碱性磷酸酶(AKP)活性显著或极显著高于Ⅰ、Ⅱ组(P0.05或P0.01)。通过二次曲线拟合分析,饲粮锌添加水平为51.38 mg/kg时,血清AKP活性最大。3)Ⅳ组的血清和肝脏总抗氧化能力(T-AOC)显著高于Ⅱ组(P0.05),与Ⅰ组差异不显著(P0.05)。通过二次曲线拟合分析,饲粮锌添加水平分别为50.24和47.49 mg/kg时,血清和肝脏的T-AOC最大。4)Ⅲ~Ⅵ组的胸腺指数和法氏囊指数均显著或极显著高于Ⅰ、Ⅱ组(P0.05或P0.01);Ⅳ~Ⅵ组免疫前后的血清新城疫抗体水平均显著或极显著高于Ⅰ、Ⅱ组(P0.05或P0.01)。由此可见,饲粮中添加植酸酶可以提高1~4周龄五龙鹅的生长性能,促进胫骨发育,增强机体抗氧化能力和免疫性能,有效提高锌利用率,降低饲粮中锌添加水平,增加锌的生物学效价。在饲粮添加1 200U/kg植酸酶的条件下,锌适宜添加水平为44.70~51.38 mg/kg。     

过瘤胃脂肪对林麝血液指标的影响

选用20头年龄相近、体况良好的成年雄性林麝。采用完全随机设计分为4组,研究日粮添加过瘤胃脂肪(0、5、10、15 g/d)对林麝血液指标的影响。结果表明:日粮添加不同水平过瘤胃脂肪对林麝血清葡萄糖、总蛋白、白蛋白、甘油三酯、胆固醇、钙、磷、镁、肌酐、孕酮等无显著影响(P0.05);试验后10 g/d组和15 g/d组雌二醇显著高于对照组(P0.05),但与5 g/d组差异不显著(P0.05);10 g/d组睾酮较对照组和5 g/d组显著提高(P0.05),但与15 g/d组差异不显著(P0.05)。根据试验结果推断,林麝日粮过瘤胃脂肪的适宜添加水平为10 g/d。     

烟酸对产蛋期种鹅繁殖性能、血清生殖激素、血清抗氧化能力及养分利用率的影响

试验旨在研究饲粮中不同烟酸添加水平对产蛋期种鹅繁殖性能、血清生殖激素、血清抗氧化能力及养分利用率的影响。将180只体况相近的34周龄五龙鹅分为6组,每组6个重复,每个重复5只（4母1公）,各组分别在基础饲粮（烟酸含量为29.16 mg/kg）中添加烟酸0、10、20、30、40、50 mg/kg。预试期7 d,正试期70 d。结果表明：烟酸对产蛋期种鹅繁殖性能和血清生殖激素均无显著影响;50 mg/kg组血清总抗氧化能力高于0、10 mg/kg组（P<0.01）,20、30、40 mg/kg组血清总抗氧化能力高于0、10 mg/kg组（P<0.05）;拟合回归方程计算显示,当饲粮中烟酸添加量为44.5 mg/kg时,血清总抗氧化能力最高;30、40、50 mg/kg组粗纤维的表观利用率高于0 mg/kg组（P<0.05）;40 mg/kg组和50 mg/kg组粗脂肪的表观利用率高于0mg/kg组（P<0.05）;40mg/kg组和50mg/kg组磷的表观利用率高于0mg/kg组（P<0.05）。由此可见,基础饲粮中添加40～50 mg/kg烟酸（即饲粮中烟...     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.     

Mechanism of Action of Probiotic Function of Lactobacilli

乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制.     

Evaluation of serum concentrations of biomarkers of skeletal metabolism and results of radiography as indicators of severity of osteochondrosis in foals

OBJECTIVE: To determine whether serum concentrations of biomarkers of skeletal metabolism can, in conjunction with radiographic evaluation, indicate severity of osteochondrosis in developing horses. ANIMALS: 43 Dutch Warmblood foals with varying severity of osteochondrosis. PROCEDURE: 24 foals were monitored for 5 months and 19 foals were monitored for 11 months. Monthly radiographs of femoropatellar-femorotibial and tibio-tarsal joints were graded for osteochondral abnormalities. Serial blood samples were assayed for 8 cartilage and bone biomarkers. At the end of the monitoring period, foals were examined for macroscopic osteochondrosis lesions. RESULTS: Temporal relationships were evident between certain serum biomarkers and osteochondrosis severity in foals during their first year. Biomarkers of collagen degradation (collagenase-generated neoepitopes of type-II collagen fragments, type-I and -II collagen fragments [COL2-3/4C(short)], and cross-linked telopeptide fragments of type-I collagen) and bone mineralization (osteocalcin) were positive indicators of osteochondrosis severity at 5 months of age. In foals with lesions at 11 months of age, osteochondrosis severity correlated negatively with COL2-3/4C(short) and osteocalcin and positively with C-propeptide of type-II procollagen (CPII), a collagen synthesis marker. Radiographic grading of osteochondrosis lesions significantly correlated with macroscopic osteochondrosis severity score at both ages and was strongest when combined with osteocalcin at 5 months and CPII at 11 months. CONCLUSIONS AND CLINICAL RELEVANCE: The ability of serum biomarkers to indicate osteochondrosis severity appears to depend on stage of disease and is strengthened with radiography. In older foals with more permanent lesions, osteochondrosis severity is significantly related to biomarker concentrations of decreased bone formation and increased cartilage synthesis.