B细胞亚群与牛白血病发生的关系

对8头流行性白血病病牛,用8种单克隆杭体（McAb）,经过免疫组织化学ABC法和免疫荧光流式细胞检测法,观察了其免疫病理组织变化和外周血及肿瘤组织的B细胞亚群变化。结果7例病牛的BoCD5、BoCD11b和sIgM为阳性反应,表明肿瘤细胞来源于B1a细胞;1例病牛的BoCD11b和sIgM呈阳性反应,而BoCD5则呈阴性反应,说明该肿瘤细胞来源于B1b细胞。另外,在免疫组织化学染色肿瘤组织切片上在浸润的淋巴细胞中不仅见有较多的CD3和CD5阳性细胞,而且也见有较多的CD3和CD5阳性肿瘤细胞。表明流行性牛白血病的肿瘤细胞主要来源于Bla细胞,其他类型的淋巴细胞也有可能突变为白血病的肿瘤细胞。     

流行性白血病病牛的免疫病理学观察

用８种单克隆抗体（ＴＨ１４Ｂ、ＢＡＱ４４Ａ、ＢＩｇ４５Ａ、ＢＩｇ７１５Ａ、ＢＩｇ５０１Ｅ、ＣＡＣＴ１０５Ａ、ＭＭ１Ａ、ＡＨ－ＣＣ１２５）结合常规病理学方法,对１０头流行性白血病病牛的免疫病理学进行了观察。结果：患病较轻时,淋巴结内的淋巴小结肿大,生发中心明显,副皮质区显著增宽。脾脏中的脾小体亦增大,动脉周围淋巴鞘增厚。用ＭｃＡｂ证明,在淋巴结和脾脏有较多的Ｂ淋巴细胞和大量Ｔ淋巴细胞。患病较重时,淋     

乌拉圭牛地方流行性白血病传入风险分析

为评估乌拉圭牛地方流行性白血病(enzootic bovine leukosis,EBL)传入我国的风险,根据世界动物卫生组织(OIE)传入风险分析基本原理,从入境评估、暴露评估及后果评估三方面,对从乌拉圭传入EBL的风险进行分析.结果显示:从乌拉圭进口活牛、牛精液/胚胎、牛乳制品传入释放牛白血病病毒(bovine l...     

T、B淋巴细胞功能对流行性牛白血病发生发展的影响

本文回顾了T、B淋巴细胞功能与牛白血病发生发展的相关性。阐明了感染BLV牛B细胞淋巴瘤细胞除了来源于Bla(CD5^ CD11b^ )细胞，也可来源于B1b(CD5^-CD11b^ )及常规B2（CD5^-CD11b^-)细胞。探讨了T细胞功能对牛白血病的发生发展的影响，指出IL－12表达下降和IL－10表达增加可能导致了B细胞淋巴肉瘤的形成，Ⅰ型和Ⅱ型细胞因子的不平衡引起了BLV的发展。同时讨论了B－T细胞的相互作用在BLV感染时疾病阶段性发展过程中的作用。     

Studies on Bovine Leukosis

Summary

An enzyme linked immunosorbent assay (ELISA) and the agar gel immunodiffusion test with bovine leukosis virus glycoprotein as antigen (AGIDT‐BLV gp) were further used to test 633 bovine sera for antibodies to BL V. Both tests detected the same number of sera positive (149) or negative (464) for antibodies. Nine sera were negative in the ELISA but found to be weakly positive (2 sera) or bending the control line (7) in the AGIDT‐BLV gp. On the other hand 11 sera were scored negative in the AGIDT‐BLV gp but were weakly positive (9 sera), positive (1), and strongly positive (I) in the ELISA. Both tests are used routinely in this Institute as they complement each other, specially if sera with low antibody titers are under investigation. It is concluded that ELISA can fully replace radioimmunoassays in the serodiagnosis of enzootic bovine leukosis.     

T淋巴细胞亚类在地方流行性牛白血病牛外周血及脾脏中的分布

应用抗牛白细胞分化抗原单克隆抗体经免疫组织学方法及流体细胞计数器法对地方流行性牛白血病病牛（以下称牛白血病牛）,健康成年牛（以下称成年牛）及健康未成年牛（以下称未成年牛）的外周血和脾脏的Ｔ淋巴细胞亚类进行了分析。无论是在外周血还是脾脏,牛白血病牛和成年牛的Ｔ淋巴细胞亚类的百分率（ＣＤ３＋,ＣＤ４＋,ＣＤ８＋和ＷＣ１＋γδＴ淋巴细胞）均明显低于未成年牛。虽然牛白血病牛外周血中Ｔ淋巴细胞亚类的百分率低于成年牛,但在脾脏却高于成年牛。这提示在脾脏存在一定程度的肿瘤免疫反应。病理组织学观察证实脾脏未见有滤泡增生,增生的肿瘤细胞主要见于红髓。因此认为脾脏不是牛白血病淋巴肉瘤的原发器官,肿瘤细胞是从外周血中转移而来。     

An Outbreak of Enzootic Bovine Leukosis in Upper Egypt: Clinical,Laboratory and Molecular–Epidemiological Studies

In 1989, 220 Holstein Friesian cattle (212 heifers and eight bulls) were imported from Minnesota, USA, to form a closed dairy herd in Arab El‐Aoumar, Assiut, Upper Egypt. In November 1996, some abnormal signs such as loss of weight, decreased milk yield, external lymphadenopathy and decreased appetite were observed on this farm. Serological screening by enzyme‐linked immunosorbent assay revealed a seroprevalence of antibodies directed against bovine leukaemia virus (BLV) of 37.7% in cattle under 2 years old and of 72.8% in animals more than 2 years old. Diagnosis was confirmed by the detection of BLV proviral DNA using polymerase chain reaction with primers amplifying a fragment of the env gene. Out of 21 tested leucocyte fractions from individual animals, 15 were positive showing a BLV‐specific amplicon of 444 base pairs. Analysis of the amplicons for restriction fragment length polymorphisms and DNA sequencing results allowed the isolates to be typed. Since this was the first recorded case of enzootic bovine leukosis in Upper Egypt, strict quarantine measures were adopted and all serologically positive animals in the herd were culled.     

Distribution of T-lym phocyte Subpopulation in Blood and Spleenof Norm al Cattle and Catt1e w ith Enzootic Bovine Leukosis

Distribution of T-lym phocyte Subpopulation in Blood and Spleenof Norm al Cattle and Catt1e w ith Enzootic Bovine Leukosis@WUD$DepartmentofVeterinayPathology!FacultyofAgriculture!IwateUniversity,Morioka0208550,Japan@TakahashiK$DepartmentofVeterinayPathology!FacultyofAgriculture!IwateUniversity,Morioka0208550,Japan@LIUN$DepartmentofVeterinayPathology!FacultyofAgriculture!IwateUniversity,Morioka0208550,Japan@OKoguch1$DepartmentofVeterinayPathology!FacultyofAgri…     

注意防治牛流行热

牛流行热也叫牛三日热或称牛暂时热，是由牛流行热病毒引起的奶牛、黄牛及水牛的急性热性传染病。其特征为突然高热，呼吸迫促，消化机能和四肢关节障碍。本病１８６７年在西非首次发现，至今已有百余年历史，可称为老病。１９４６～１９５１年在日本暴发流行，１９７１年分离到病毒。该病已在非洲、亚洲和澳洲等许多国家发生。１９７６年，在我国华北暴发本病，并在北京分离到病毒。该病虽然死亡率不高，但传播快，牛发病率高，致使奶牛的产奶量及奶的质量明显下降，耕牛不能使役，部分母牛发生流产，病牛因体弱而被淘汰，给养牛业造成很大…