Image analysis for non‐destructive and non‐invasive quantification of root growth and soil water content in rhizotrons

Studies aiming at quantification of roots growing in soil are often constrained by the lack of suitable methods for continuous, non‐destructive measurements. A system is presented in which maize (Zea mays L.) seedlings were grown in acrylic containers — rhizotrons — in a soil layer 6‐mm thick. These thin‐layer soil rhizotrons facilitate homogeneous soil preparation and non‐destructive observation of root growth. Rhizotrons with plants were placed in a growth chamber on a rack slanted to a 45° angle to promote growth of roots along the transparent acrylic sheet. At 2‐ to 3‐day intervals, rhizotrons were placed on a flatbed scanner to collect digital images from which root length and root diameters were measured using RMS software. Images taken during the course of the experiment were also analyzed with QUACOS software that measures average pixel color values. Color readings obtained were converted to soil water content using images of reference soils of known soil water contents. To verify that roots observed at the surface of the rhizotrons were representative of the total root system in the rhizotrons, they were compared with destructive samples of roots that were carefully washed from soil and analyzed for total root length and root diameter. A significant positive relation was found between visible and washed out roots. However, the influence of soil water content and soil bulk density was reflected on seminal roots rather than first order laterals that are responsible for more than 80 % of the total root length. Changes in soil water content during plant growth could be quantitifed in the range of 0.04 to 0.26 cm³ cm^—3 if image areas of 500 x 500 pixel were analyzed and averaged. With spatial resolution of 12 x 12 pixel, however, soil water contents could only be discriminated below 0.09 cm³ cm^—3 due to the spatial variation of color readings. Results show that this thin‐layer soil rhizotron system allows researchers to observe and quantify simultaneously the time courses of seedling root development and soil water content without disturbance to the soil or roots.     

Estimation of clay content from easily measurable water content of air‐dried soil

Soil texture is one of the main factors controlling soil organic carbon (SOC) storage. Accurate soil‐texture analysis is costly and time‐consuming. Therefore, the clay content is frequently not determined within the scope of regional and plot‐scale studies with high sample numbers. Yet it is well known that the clay content strongly affects soil water content. The objective of our study was to evaluate if the clay content can be estimated by a simple and fast measure like the water content of air‐dried soil. The soil samples used for this study originated from four different European regions (Hainich‐Dün, Germany; Schwäbische Alb, Germany; Hesse, France; Bugac, Hungary) and were collected from topsoils and subsoils in forests, grasslands, and croplands. Clay content, water content of air‐dried soil, and SOC content were measured. Clay content was determined either by the Pipette method or by the Sedigraph method. The water content of air‐dried soil samples ranged from 2.8 g kg^–1 to 63.3 g kg^–1 and the corresponding clay contents from 60.0 g kg^–1 to 815.7 g kg^–1. A significant linear relationship was found between clay content and water content. The scaled mean absolute error (SMAE) of the clay estimation from the water content of air‐dried soil was 20% for the dataset using the Pipette method and 28% for the Sedigraph method. The estimation of the clay content was more accurate in fine‐textured than in coarse‐textured soils. In this study, organic‐C content played a subordinate role next to the clay content in explaining the variance of the water content. The water retention of coarse‐textured soils was more sensitive to the amount of organic C than that of fine‐textured soils. The results indicate that in our study the water content of air‐dried soil samples was a good quantitative proxy of clay contents, especially useful for fine‐textured soils.     

Analyzing the efficiency of soil amendments and irrigation for plant production on heterogeneous sandy soils under greenhouse conditions

Variability in soil properties is a complication for fertilization, irrigation, and amendment application. However, only limited progress has been made in managing soil variability for uniform productivity and increased water‐use efficiency. This study was designed to ameliorate the poor‐productivity areas of the variable sandy soils in Florida citrus groves by using frequent small irrigations and applying organic and inorganic soil amendments. Two greenhouse experiments were set up with sorghum and radish as bioassay crops in a randomized complete block design (RCBD). The factors studied were two soil‐productivity classes (very poor and very good), two water contents (50% and 100% of field capacity), two amendments (phosphatic clay and Fe humate), and two amendment rates (10 and 25 g kg^–1 for sorghum and 50 and 100 g kg^–1 for radish). Amendments applied at 50 and 100 g kg^–1 increased the water‐holding capacity (WHC) of poor soil by 2‐ to 6‐fold, respectively. The lower rates (10 and 25 g kg^–1) of amendments were not effective in enhancing sorghum growth. The higher rates (50 and 100 g kg^–1) doubled the radish growth as compared to the control. The results indicate that rates greater than 50 g kg^–1 of both amendments were effective in improving water retention and increasing productivity. Irrigation treatment of 100% of field capacity (FC) increased the sorghum and radish growth by about 2‐fold as compared with the 50%–water content treatment. The results suggest that the root‐zone water content should be maintained near FC by frequent small irrigations to enhance water availability in excessively drained sandy soils. In addition, application of soil amendments in the root zone can enhance the water retention of these soils. Furthermore, managing variable sandy soils with WHC‐based irrigation can increase water uptake and crop production in the poor areas of the grove.     

Small Scale Heterogeneity of Soil Chemical Properties: Effects on Spring Water Chemistry

Previous soil and spring water analyses in small catchments revealed low pH values in the spring water during high discharge events. This paper analyses the potential which small scale heterogeneity of soil acidity may have to explain decreasing spring water pH as a result of high discharge. Soil aggregates were collected from a C‐horizon of a Spodosol in the Fichtelgebirge. Exchangeable cations and soil solution were examined on both samples from the surfaces and the cores of aggregates which were obtained by a mechanical separation procedure. The Reuss‐Johnson soil chemical equilibrium model was used to predict soil and spring water pH values as a function of acidic input and soil air CO₂ concentration in equilibrium with both aggregate fractions. Ranges of acidic input from 160—570 μeq L⁻¹ and soil air CO₂ concentrations from 0.1 to 3 Vol. % were considered. The model predicted spring water pH values from 5.0 to 5.3 for the acidic aggregate surface samples (base saturation = 12.5%) and from 6.8 to 7.2 for the aggregate core samples (base saturation = 32.1%). The results suggest that small scale acidity gradients may expand the range of predictable spring water pH values. However, very low pH values (<5) still need additional explanation.     

Effects of surface‐applied and soil‐incorporated lime on some physical attributes of a Dystrudept soil

It is generally accepted that liming ameliorates soil acidity. However, the method of lime application is thought by many to influence its effectiveness in acid soils. In this study, we wanted to assess the degree of effectiveness of surface‐applied lime and lime incorporated into the soil on soil structural attributes and water retention of a Dystrudept soil in the SE region of the State of Paraná, Brazil. Lime was added at the rate of 15 t/ha to soil through: (i) surface broadcasting, (ii) incorporation via ploughing and harrowing, (iii) incorporation via subsoiling and harrowing. A control treatment with zero lime application was included in the experiment. The addition of lime by surface broadcasting resulted in significant reductions in soil bulk density (BD) and macroporosity (Ma) and increases in total porosity (TP) and microporosity (Mi) of the top soil layer (0–0.10 m). The reverse was the case in the 0.10‐ to 0.20‐m soil layer; where lime was incorporated via ploughing and harrowing, increases in BD and reductions in TP and Ma were observed. Addition of lime also significantly increased soil water retention, with maximum retention recorded from soil amended with surface broadcast lime. Changes in soil chemical attributes (increases in pH, Ca²⁺ and Mg²⁺ contents; reductions in potential acidity and Al³⁺ content) were responsible for the changes observed in structural and physical attributes, and water retention. Bearing in mind the lower application costs, improvement in the soil chemical attributes for plant development and soil physical quality, surface broadcast lime can be considered a promising alternative for no‐till farmers.     

Characterization of Pterocarpus macrocarpus (pradoo wood) biochar and its effect on the retention properties of sandy soils in Northeast Thailand

Pradoo wood biochar has been tested in order to explore sustainable solutions to the development of agriculture on poor sandy soils in marginal areas in Northeast Thailand. Some basic physicochemical properties of biochar, for example pore size distribution, cation exchange capacity (CEC), specific surface area (SSA), and water and nutrient adsorption, were determined and compared to soil properties in order to determine appropriate biochar application to soil. Pradoo wood biochar showed important adsorption properties with high SSA, CEC and nutrient adsorption. The water retention properties were also improved on the dry end of the water retention curve. Phosphorous and ammonium adsorption–desorption isotherms were established and their respective affinity for the biochar surface was quantified, by the means of a retention index and thermodynamical parameters. We found that despite excellent retention properties, biochar needs to be added in large amounts (between 10 and 70 kg m⁻²) to soil to be able to modify noticeably the resulting soil properties.     

Prediction of long‐term sustainability of constructed urban soil: impact of high amounts of organic matter on soil physical properties and water transfer

Sustainability of urban soils lies in their ability to facilitate water and air permeabilities. Exogenous organic matter has been shown to have a positive impact on these properties. Under urban conditions, a large one‐time input of an organic amendment was made to the reconstituted soil. Two organic materials, green‐waste compost (gw) or cocompost from sewage sludge and wood chips (sw), were mixed with sandy loam soil (40% v/v) and placed in 600‐L containers. Containers received a 29‐cm thick layer of sandy loam soil–organic matter mix over a 28‐cm thick layer without organic amendment. Volumetric water content, dry bulk density, hydraulic conductivity at saturation and water retention were measured over 5 yrs in the soils and values for the mixes and a control compared. After this time, dry bulk density was greater (1.54 g/cm³) in control than in gw or sw soils (1.31 and 1.11 g/cm³, respectively), whereas hydraulic conductivity at saturation was smaller (4 × 10^?7 m/s) than in gw (3.4 × 10^?6) or sw (3.7 × 10^?6 m/s). HYDRUS 1D water balance model indicated that below 27 cm depth in the control after 5 yrs, there was a high degree of anoxia, lasting >200 days per year, compared with <40 days in gw and sw. Amplification of the risk of anoxia below 27 cm depth after 10 yrs was 323, 151 and 100 days in the control, gw and sw, respectively. Organic matter amendment could support sustainable urban soils for ten years after soil reconstitution.     

Arsenic in field‐collected soil solutions and extracts of contaminated soils and its implication to soil standards

Large concentrations of arsenic in soils, sediments, and freshwaters require risk assessment across the Central Alps and other regions. We measured arsenic concentrations in soil samples collected from 38 sites located in the Austrian Central Alps that had been contaminated due to mining and smelter activities and geogenic mineralization. Medians and ranges of arsenic concentrations (in mg kg^—1) in the soil solid phase were: 77.1 (1—3000) for the total (As_t), 19.2 (0—726) for (NH₄)₂C₂O₄‐extractable (As_o), 2.35 (0—169) for (NH₄)₂HPO₄‐extractable (As_p), and 0.143 (0—11.1) for (NH₄)₂SO₄‐extractable (As_s) arsenic. Arsenic concentrations in soil solutions (As_sol) collected from organic surface layers and mineral horizons at five selected sites using suction cups fitted with nylon membranes ranged from 0 to 171 μg l^—1. Typically, the prevailing species of As in the soil solution was As(V). As_sol was correlated with As_s (As_sol = 0.279 + 15.6 As_s; r² = 0.938; n = 17) and As_t (As_sol = 1.272 + 0.043 As_t; r² = 0.833; n = 17). Using these empirical models, As_sol can be predicted quite accurately based on extraction with 0.05 M (NH₄)₂SO₄ or total arsenic concentrations in the soil. Linking these models to drinking water standards (DWS) we propose soil standards for freshwater protection that vary for As_s (mg kg^—1) between 0.62 (for DWS = 10 μg l^—1 WHO) and 3.19 (for DWS = 50 μg l^—1). Corresponding standards for As_t (mg kg^—1) are 203 (DWS = 10 μg l^—1) and 1133 (DWS = 50 μg l^—1). These considerations demonstrate that changes in legislation on DWS may have dramatic impact on As concentrations in soil that are acceptable for groundwater protection.     

The interrelationship between the cultivation of crops and soil‐strength dynamics

It is well accepted that the penetration resistance of soils is, among others factors, highly sensitive to the moisture status of the soil. This study tested the hypothesis of whether the dewatering of a soil by crops of varying dewatering capacities significantly affects the soil's penetration resistance and whether this contributes to an exceedance of the commonly accepted root‐growth threshold already in the range of plant‐available water. During a 22‐month period between March 2002 and December 2003, the soil water content of a former lignite strip mine in E Germany was studied. The soil had been restored with Saalian glacial till. Plots contained two different crops, a 3 y–old stand of lucerne (Medicago sativa L.) and a 7 y–old stand of wild rye (Secale multicaule L.). Soil water contents under the two crops were converted on the basis of the water‐retention characteristics into water tensions, allowing an investigation of the changes in the measured water content in the wider context of the water availability to the crops. During both growing seasons, the water tension under lucerne exceeded the permanent‐wilting point (10^4.2 hPa) for up to 20 weeks between 0 and 90 cm, which is equal to a predicted penetration resistance of >15 MPa. Water tensions under the wild rye rose only up to a maximum of 10³ hPa for the same period, so that the predicted penetration‐resistance values remained constantly <5 MPa. Our findings demonstrate that the dewatering by plants during the growing seasons affects the actual strength of the soil, which can lead to the exceeding of the commonly accepted root‐growth threshold.     

Laboratory method for determining immobile soil water content and mass exchange coefficient

Preferential flow in soil can enhance the leaching of agricultural chemicals. In a number of studies it has been shown that the mobile‐immobile solute transport model (MIM) is a useful tool to characterize preferential flow. In the present study, a new laboratory method for determining the MIM parameters θ_m and θ_im (mobile and immobile water content), as well as α (mass transfer coefficient), is developed. The computations are uncomplicated and the method requires only simple equipment. It is applied to short, undisturbed soil columns. Measured values ranged from 0.11 to 0.27 for θ_im θ^—1 and from 0.015 h^—1 to 0.034 h^—1 for α for an Iowan soil (Nicollet silt loam). For two sandy Eutric Gleysols from Germany, low values for θ_im θ^—1 from 0.04 to 0.07 and from 0.001 h^—1 to 0.008 h^—1 for α were determined. Although the new method is a flow‐interruption technique, values for the Nicollet silt loam compare well with those from conventional leaching experiments. Values for the Eutric Gleysols agree with the observation that these soils were poorly structured. Because the new method does not assume negligible dispersion, it is applicable to a wider range of soils and boundary conditions than comparable approaches. We conclude that the new method provides parameter values that are suited to describe non‐equilibrium solute transport.     

Soil physical characteristics of peat soils

Drainage and intensive use of fens lead to alterations in the physical characteristics of peat soils. This was demonstrated using parameters of water balance (available water capacity) and the evaluated unsaturated hydraulic conductivity. Deriving the distribution of the pore size from the water retention curve was flawed because of shrinkage due to drainage, especially at high soil water potentials. These errors became greater as the peat was less influenced by soil‐genetic processes. The water retention curves (desorption) evaluated in the field and the laboratory satisfactorily corresponded. However, the wetting‐ and drainage‐curves obtained in the field differed up to 30 vol.‐% water content at same soil water potentials. These differences were largely due to a wetting inhibition.     

Polyethersulfone Membrane Filters for Sampling Soil Water from In Situ Soils and Intact Soil Columns for Phosphate Analysis

Abstract

Porous plates or cups are commonly used to collect soil solution samples in field studies or from intact soil columns. Some commonly used materials for porous plates may adsorb soil solution constituents such as phosphorus (P). An alternative to using a porous plate is to use a membrane filter with a known pore size and bubble point. The objective of this study was to evaluate the utility of polyethersulfone membranes (pore size 0.45 µm and bubble point >200 kPa) to extract soil solution from in situ soils and intact soil columns for phosphate analysis. In situ soil solution samplers were constructed from modified reusable polysulfone membrane filter holders equipped with polyethersulfone membranes (47 mm diameter). A ?10 kPa vacuum was maintained in the samplers, which enabled soil solution collection at soil water potentials of 0 to ?4 kPa in loamy sand, 0 to ?10 kPa in sandy loam, and 0 to ?12 kPa in sandy clay loam soils. In a laboratory study, soil solution samplers continued to hold a vacuum to ?77 kPa soil water potential. Soil solution samplers were further evaluated in a field study at 45‐, 90‐, and 135‐cm depths in two soils. Samplers operated with relatively few difficulties for the first 12 months of field evaluation. Membranes apparently dried during periods of low soil water potential but increases in soil moisture were sufficient to rewet the membrane. Sampler failures in the field increased during 13–18 months because aged vacuum tubing and root interferences with samplers at 45 cm. Improvements in sampler design may improve the durability for implementation in long‐term field experiments. Membrane filters worked near flawlessly to maintain unsaturated conditions in intact soil columns. The filter units facilitated easy collection of soil water from the intact soil columns without altering the chemical composition of the percolate.     

Field calibration of an impedance soil water probe for the shallow seedbed across field topography

Impedance soil water probes enable frequent and non‐destructive determination of soil water status in situations where gravimetric soil sampling is too demanding of time and sampling space. The ThetaProbe is an impedance soil water probe requiring calibration for local soil conditions, because measurement accuracy can be affected by properties of the soil. Often, only a single calibration is performed for an experimental site. An experiment investigating the seedbed to 75‐mm depth across a field topography with variable soil properties was examined to determine which soil properties affected the calibration of the ThetaProbe, and if soil‐specific calibration was required to derive suitable estimates of the water status in the experiment. Experimental factors examined included hillslope aspect, hillslope position, crop residue and soil depth. Soil properties, other than volumetric water content, significantly affecting the probe measurements were bulk density, electrical conductivity and temperature. The probe underestimated soil water at very low water contents, and overestimated soil water at contents greater than 11 m³ m^?3, compared with gravimetric measurements. A single calibration, not corrected for hillslope position at a water content of 20 m³ m^?3, overestimated water content by 0.02 m³ m^?3 in the summit hillslope position and underestimated water content by 0.04 m³ m^?3 in the toeslope position. A single calibration, not corrected for soil depth at a water content of 20 m³ m^?3, overestimated water content by 0.02 m³ m^?3 in the 0‐ to 25‐mm soil layer and underestimated water content by 0.03 m³ m^?3 in the 50‐ to 75‐mm layer. The complexity of microsites in a shallow seedbed requires soil‐specific calibration in field experiments containing heterogeneous soil properties.     

祁连山南麓高寒禾草-矮嵩草草甸土壤水源涵养功能的特征

青藏高原被誉为“中华水塔”,高寒草甸是主要植被类型但其水源涵养功能有待准确量化。以祁连山南麓高寒禾草-矮嵩草草甸为研究对象,通过分析2014—2018年的植被生长季(6—9月)土壤体积含水量的长期观测数据,探讨了土壤有效水源涵养量(土壤现实持水量与最小持水量之差)和水文调节功能(有效水源涵养量的时间变化速率)的变化特征及其环境调控机制。结果表明:高寒草甸0—100 cm年均土壤有效水源涵养量为(44.3±8.7)mm(平均值±标准差,下同),呈现出双峰型的季节趋势,最高峰和次高峰分别为6月下旬的(57.8±14.4)mm和9月中旬的(59.2±15.7)mm。浅层(0—20 cm)、中层(20—60 cm)和深层(60—100 cm)土壤有效水源涵养量占比分别为53.1%,34.9%和12.0%,土壤有效含水源涵养量随土层深度增加表现为对数衰减(R²=0.82,p<0.001)。增强回归树的结果表明土壤有效水源涵养量的季节变化主要受控于土壤温度,尤其是5 cm土壤温度,二者呈现出显著负相关。不同深度的年均土壤有效水源涵养量和土壤黏粒比例显著负相关(R²=0.99,p=0.004)。根系区(0—40 cm)年均土壤吸湿速率和脱湿速率分别为(0.21±0.02)mm/h和(0.22±0.02)mm/h,t检验的结果表明除了0—5 cm之外,根系区土壤脱湿速率显著大于吸湿速率。分析表明土壤温度是土壤吸湿和脱湿速率的显著环境驱动因子。因此,土壤温度是高寒禾草-矮嵩草草甸土壤有效水源涵养量和水文调节功能的主要影响因素,维持土壤的低温是高寒草甸水源涵养功能保育和提升的重要基础。     

A review on recent developments in soil water retention theory: interfacial tension and temperature effects

Study of soil physical processes such as water infiltration and redistribution, groundwater recharge, solute transport in the unsaturated zone, compaction and aeration in variably saturated soil hardly is possible without knowledge of the capillary pressure of the soil water as a function of the degree of saturation. Pore space topology, interfacial tension, and temperature probably are the most important physical factors affecting the capillary pressure at a given water content. Despite intensive research in the past decades on the water retention characteristics of soils, our knowledge of their response to varying ambient conditions is far from being complete. Current models of soil water retention as well as of hydraulic conductivity for unsaturated porous media often still use the simplified representation of the pore system as a bundle of cylindrical capillaries. Physical effects, like surface water film adsorption, capillary condensation and surface flow in liquid films, as well as volumetric changes of the pore space are often ignored. Consequently, physical properties of the solid phase surfaces, and their impact on water adsorption and flow, are often not considered. The objective of this contribution is to review various interfacial properties with possible application to the conventional water content — matric potential relation of soils. The ignoring of inter‐facial effects on the water retention of soils is widespread in the literature. The motivation of this paper is therefore to point out some of the more significant deficiencies of our current knowledge on the interaction of solid particle surfaces and the liquid phase in soil. We will first emphasize the impact of the wetting angle on the wetting of dry soil and to present the impact of interfacial tension of the liquid phase in the three‐phase system. At low water content, the transition from capillary‐bound water to adsorbed water and to wetting films is discussed separately, because of its impact on the rewetting process of dry soil. Finally, we discuss the impact of temperature on interfacial tension and water retention of soil as a second important interfacial process affecting directly the water retention of porous media.     

Pressurized Hot Water and DTPA‐Sorbitol as Viable Alternatives for Soil Boron Extraction. I. Boron‐Treated Soil Incubation and Efficiency of Extraction

Abstract

Serious challenges associated with hot water extraction, the standard extraction method for water‐soluble boron (B), limit its use in commercial soil‐testing laboratories. Several alternatives to make B testing more practical have been proposed and studied; none of the alternatives have readily replaced the hot water method. Two relatively new, promising B extraction methods are pressurized hot water and DTPA‐Sorbitol. Very little reported work compares B extraction values obtained from the standard hot water extraction method and these two alternative methods. This study was conducted to complete an initial step in validating new procedures—extracting the designated nutrient from fertilized, incubated soils by using standard and alternative extraction methods and comparing the resulting values. The three extraction methods were used to extract B from samples of calcareous sand and silt loam soils and limed, loamy fine sand, all which had been treated with 10 levels of B (0–8 mg kg^?1) and incubated for 7 and 28 days. The amount of B extracted increased as the rate of B application increased with all three soil‐extraction methods. High correlations (r 0.977–0.999) were observed between extractable B and rate of B application with all three procedures. Correlations between the amount of extractable B using hot water extraction and the value obtained with an alternative extraction method were similar for both methods (r=0.89). Hot water generally extracted the least and pressurized hot water the most B regardless of soil type, rate of application, or duration of incubation. This study suggests the more easily used methods of pressurized hot water and DTPA‐Sorbitol could be recommended as replacements to the cumbersome hot water extraction.     

Cyanide in paper de‐inking sludge used as a soil amendment

Paper de‐inking sludge is processed during the recycling of paper, and is sometimes used as a soil amendment. In this study the effect of a compost application on the cyanide (CN) status in soils of a public park was investigated. The compost was a mixture of chipped limbs and paper de‐inking sludge. Furthermore, the cyanide solubility was studied by conducting batch experiments with different pH levels. Total cyanide in the amended soils ranged from 540 to 740 mg CN kg^—1, and water soluble cyanide from 170 to 370 μg CN l^—1 as determined by means of an aqueous extract. Easily‐liberatable cyanides, which include the toxic free cyanide (HCN and CN^—) and weak metal‐cyanide complexes, were not present in the soil. From this result and the fact that iron blue pigments are used during paper printing, it can be inferred that cyanides occurring here were exclusively stable iron‐cyanide complexes [Fe(CN)₆]. With increasing pH the solubility of cyanide increased. In contrast to soils of coking plants, in which cyanide occur as Berlin blue, Fe₄[Fe(CN)₆]₃, the cyanide solubility in the paper de‐inking sludge amended soils was substantially lower, especially in the neutral and alkaline range. Thus, cyanides in paper de‐inking sludge could be present as sparingly soluble metal‐cyanide compounds with the general formula A₂B[Fe^II(CN)₆] with A = K⁺, Na⁺ and B = Ca²⁺ or divalent transition metals and B₂[Fe^II(CN)₆] with B = divalent transition metals. Pollution exposure by the pathways soil → human, and soil → air → human can be neglected. However, since leaching of iron‐cyanide complexes into the ground water cannot be excluded, and since they are decomposed to HCN when exposed to day light, environmental hazards by the pathway soil → ground water → surface water are possible. This is the risk arising from paper de‐inking sludge applications to soils.     

Effect of No‐Tillage and Conventional Tillage Systems on the Chemical Composition of Soil Solid Phase and Soil Solution of Brazilian Savanna Oxisols

No‐tillage (NT) cropping systems are becoming increasingly important in the Brazilian savanna. To evaluate their sustainability we compared soil chemical properties in 1‐ to 3‐year‐old NT systems following 9 to 11 years of conventional tillage (CT) with systems where CT was continuously in place for 12 years. In the rainy season 1997/98, NT was cropped with soybean and CT with corn while in the rainy season 1998/99 both systems were cropped with soybean. Soil solid phase samples were taken from the 0—0.15, 0.15—0.3, 0.3—0.8, 0.8—1.2, and 1.2—2 m layers on three spatially separated plots under each of NT and CT. Soil solution samples were collected weekly at 0.15, 0.3, 0.8, 1.2, and 2 m soil depth during two rainy seasons (14 October to 28 April 1997/98 and 1998/99). We determined soil moisture contents, pH, the concentrations of exchangeable cations, the electrical conductivity (EC) of the soil solution, and the concentrations of Al, C, Ca, Cl^—, K, Mg, Mn, Na, NH₄⁺, NO₃^—, P, S, and Zn in solid soil and soil solution samples. Differences in soil solid phase properties and moisture content between NT and CT were small, few were significant. Under NT, the average solution pH was significantly lower (5.5), Al (26 μg l^—1), Mn (17 μg l^—1) and total organic C concentrations (TOC, 6.5 mg l^—1) were higher than under CT (pH: 6.0, Al: 14μg l ⁻¹, Mn: 14μg l ⁻¹, TOC: 5.5 mg l ⁻¹). Irrespective of the different crops in the first rainy season, under NT, the EC (205 μS cm^—1), Ca (17 mg l^—1), and Mg (2.9 mg l^—1) concentrations at 0—0.3 m depth were lower than under CT (EC: 224 μS cm^—1, Ca: 25 mg l^—1, Mg: 5.6 mg l^—1). At 1.2—2 m depth, the reverse order was observed (EC: 124 μS cm^—1 under NT and 84 μS cm^—1 under CT, Ca: 11 mg l^—1 under NT and 7.5 mg l^—1 under CT, Mg: 3.1 mg l^—1 under NT and 1.8 mg l^—1 under CT). Our results indicate that enhanced soil acidification because of higher rates of organic matter mineralization and a more pronounced nutrient leaching because of increased pore continuity may limit the sustainability of NT.     

Long term crop management effects on soil organic carbon,structure, and water retention in a cropland soil in central Ohio,USA

The objective of this study was to determine 13‐year management effects on soil properties between a corn–soybean (Zea mays–Glycine max) cropping system (CSRS) and vegetable production systems (VPS) on a soil in central Ohio. Three treatments included in the VPS were: (1) addition of wood chips, (2) permanent raised beds (PRB) with black polyethylene film (20 μm thick), and (3) bare soil surface (BSS). Additionally, (4) animal manure was applied in all CSRS and VPS treatments except for the wood chips (WCP) added plot in the VPS. Research data from the study show that relatively more soil organic carbon (SOC) stock in the 0–20 cm soil depth of the BSS treatment (100.6 Mg ha^?1) was primarily due to differences in the type of soil amendments applied. For example, composted poultry manure was applied in the BSS and PRB plots, compared with input of fresh dairy manure mixed with straw being applied in the CSRS. Furthermore, soil management practices that aided in avoiding or reducing soil compaction (i.e., PRB or application of WCP in the surface) resulted in the overall improvement in soil structure and water retention, compared with that under chisel and disc ploughing done in the CSRS. The highest plant available water capacity (1.79 cm) was observed in the CSRS compared with 0.97 cm under BSS and PRB plots. These trends suggest that the type and amount of animal manure is critical to increasing SOC stocks in intensively cultivated VPS and CSRS in central Ohio, while also improving soil structure and water retention.     

Solubilization of iron by water‐extractable humic substances

The capability of water‐extractable humic substances (WEHS) to solubilize Fe from sparingly soluble Fe‐hydroxide was studied. Addition of WEHS (1.7 mmol organic C l^—1) to a dialysis tube containing labeled insoluble Fe‐hydroxide caused an increase in the amount of ⁵⁹Fe measured in the external solution. The humic fraction was also able to solubilize Fe from soil samples, with levels comparable to those obtained using a solution containing 100�μM DTPA. By measuring the amount of ⁵⁹Fe eluted from soil columns pre‐loaded with ⁵⁹Fe‐WEHS it was possible to evaluate the mobility of Fe complexed to the humic molecules. The recovery of ⁵⁹Fe varied from 2% to 25% in respect to the soil type used. The ability of Fe‐WEHS to serve as an Fe source for the phytosiderophore hydroxy‐mugineic acid (HMA) was also analyzed. The removal of ⁵⁹Fe from the Fe‐WEHS complex by HMA was demonstrated by adding the phytosiderophore to a dialysis tube containing the ⁵⁹Fe‐WEHS complex. The observations suggested a ligand exchange between the phytosiderophore and the humic fraction. The results indicate that WEHS is able to increase the amount of Fe present in the soil solution, possibly by forming mobile complexes with the micronutrient. These complexes could act as easily available Fe sources in Fe acquisition processes by both monocot and dicot plants, playing an important role particularly in soils with low available Fe.