Epididymal interstitial (Leydig) cell tumors in B6C3F1 mice

Six primary interstitial cell tumors of the epididymis were identified from 46,752 male B6C3F1 mice used in chronic toxicity and carcinogenicity studies. Five of the tumors occurred at the end of 2-year studies; none were attributed to treatment. None of the mice with epididymal tumors had a primary testicular tumor. Histologically, tumors were characterized by a nodular or diffuse proliferation of tumor cells in the epididymal interstitium. Most cells were polygonal with highly vacuolated cytoplasm (vacuolated cells) or eosinophilic cytoplasm (eosinophilic cells). Smaller hyperchromatic cells with scant basophilic cytoplasm (basophilic cells) and cells with yellow-brown pigment characteristic of lipofuscin (pigmented cells) were less common. In each tumor two or more cell types were present. Extension of these tumors through the capsule, invasion of the testis, or metastasis did not occur. By electron microscopy both eosinophilic and vacuolated cell types had a large round or oval nucleus with sparse heterochromatin, abundant mitochondria with tubulovesicular cristae, and frequent desmosome structures between cell membranes. Vacuolated cells contained numerous lipid droplets. Morphological features of the epididymal tumors are similar to those of the testicular interstitial (Leydig) cell tumor in mice and rats.     

Tumors and tumor-like lesions in the genitalia of sows

Of a total of 1445 sows, tumors were detected in the uterus of 11 animals and in the cervix/vagina of 1 animal. Tumor-like lesions were detected in the ovary of 56 animals. The incidence of tumors was higher than that mentioned in the literature, possibly because of the relatively high age of the sows (mean parity of 6.8) and the accuracy of investigation. Several tumors would not have been detected during routine slaughter examination. The uterine tumors were diagnosed as leiomyoma (6), fibroma (3), cyst-adenoma (1) and fibro-leiomyoma (1). The cervical/vaginal tumor appeared to be a fibroma. The ovarian lesions could not be satisfactorily classified. They consisted of connective tissue, blood vessels, and granulosa cells, which were present in variable quantities in different ovaries and in different sides of the same ovary. Sows with tumors were older than the mean determined age at slaughter (parity 6.8). Sows with uterine and cervical/vaginal tumors had a parity of 11.6; sows with ovarian lesions had a parity of 9.0. Sows with uterine- or a cervical/vaginal tumor were often slaughtered for infertility reasons.     

Non-gestational malignant placental site trophoblastic tumor of the ovary in a 4-year-old rhesus monkey

We report a case of ovarian malignant intermediate-type trophoblastic tumor in a clinically normal, nonpregnant 4-year-old rhesus monkey (Macaca mulatta). A large solid lobulated mass replaced the right ovary and filled the pelvis. Multiple metastases were observed within the lungs and the liver. The tumor was histologically identified as predominantly composed of intermediate trophoblastic cells, without prominent hemorrhages and the classic bilaminar pattern of cyto- and syncytiotrophoblastic cells characteristic of choriocarcinoma. Immunohistochemical analysis showed the presence of placental lactogen hormone in many tumor cells and chorionic gonadotropin in a few multinucleated cells consistent with syncytiotrophoblastic differentiation. No other germ cell differentiation was identified in the pelvis mass nor in the metastases. In the absence of previous and present pregnancy, this neoplasm has to be considered as a nongestational malignant placental site trophoblastic tumor of the ovary.     

Ovarian Fibrothecoma in an Arabian Mare: A Rare Case

The occurrence of ovarian tumors is uncommon in domestic animals. Reports are documented more often in the bitch, mare, and cow. In the mare, granulosa-thecal cell tumor is the most common ovarian neoplasms, and other tumors occur less often. Fibrothecoma is a rare gonadal-stromal tumor of the ovary. There is only one reported case in the veterinary literature. This tumor consists of theca cells and fibrous tissues. The present study describes a rare case of fibrothecoma in a 10-year-old Arabian mare that was presented due to infertility problems. The abnormal left ovary was detected via rectal palpation and ultrasonography. Postoperation study showed a solid tumoral mass that completely replaced the normal ovarian tissues. The tumor was large, firm in consistency, with smooth surface. On the cut surface, the mass was white-gray to yellow with cystic areas containing green gelatinous materials. Microscopic examination revealed the structure was composed of fibroblastic cell producing collagen fibers and theca cells containing lipids. Based on histopathologic features, diagnosis of fibrothecoma was confirmed postoperatively.     

Spontaneous seminoma in medaka (Oryzias latipes)

Although spontaneous development of seminoma is rare in medaka, we encountered spontaneous testicular tumors located within the abdominal cavity in two adult medakas. The growth patterns of the tumors were a combination of solid and cord arrangements in one of the two cases (Case I) and lobular in the other case (Case II). The tumor cells resembled the cells at different stages of spermatogenesis, and a small number of oocyte-like cells were also scattered within the tumor. The tumor with solid and cord patterns showed loss of normal testicular architecture, and the tumor cells had partly invaded the dorsal muscular tissue and metastasized to the liver, kidney, and eye. The tumor with a lobular pattern did not exhibit local invasion or metastasis. The tumors were diagnosed as seminomas based on their histopathological characteristics, and the tumor in Case I was observed to be more malignant than that in Case II.     

A spontaneously occurring malignant ovarian Sertoli cell tumor in a young Sprague Dawley rat

Primary ovarian tumors are generally uncommon in rats used in toxicologic studies. A malignant Sertoli cell tumor was present in the ovary of a 19-week-old female Sprague Dawley rat. Macroscopically, the mass was white and firm, 10 × 13 × 17 mm in size, and located in the right ovary. Histopathologically, the mass was composed of nests of pleomorphic cells, which formed seminiferous-like tubules separated by a thin fibrovascular stroma. The tubules were lined by tumor cells, which had basally located nuclei and abundant eosinophilic and vacuolated cytoplasm. In some areas, the tumor cells were arranged in a retiform growth pattern, mimicking a rete testis/ovarii. Disseminated metastases to the surfaces of the mesentery, spleen and liver were also present. Immunohistochemically, many tumor cells were strongly positive for vimentin, estrogen receptor α and Ki 67. Some tumor cells were positive for pancytokeratin and inhibin α. These findings closely resemble those of an ovarian-derived human malignant Sertoli cell tumor. From our review of the literature, we believe this is the first report of a spontaneous malignant Sertoli cell tumor in the ovary of a young laboratory rat. This case might provide useful historical control information for rat toxicity studies.     

Maternal exposure of low dose of TCDD modulates the expression of estrogen receptor subunits of male gonads in offspring

We have analyzed the effects of low-dose transplacental and lactational exposure of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on gene expression relating to the dioxin and sexual hormone cascade, and demonstrated the effects on testicular growth and sexual maturation in male offspring rats. TCDD (10 ng/kg) was administered to dams on Days 7 and 14 of gestation, and on Days 0, 7 and 14 after delivery. Gene expression of cytochrome P450 family 1 subfamily A polypeptide 1 (CYP1A1) in the liver of 17-day-old rats was significantly increased compared with controls. Furthermore, expression of estrogen receptors (ER)alpha and ERbeta was significantly increased at 17 and 42 days old, respectively in the testis of TCDD-administered rats compared with controls. Although testicular weight and the seminiferous tubule diameter were increased in 17-day-old rats, there was no difference in the number of germ cells between TCDD-treated and control animals. The expressions of androgen receptor and inhibin subunit genes were not significantly changed. These findings suggest that low-dose exposure of TCDD leads to unusual development of the testis by perturbation of steroid hormone homeostasis.     

Spontaneous ovarian neoplasms of the laboratory rat

A wide range of spontaneous tumors of the ovary are reported in a group of 5903 aged female Sprague-Dawley rats of the CD and CFY strains examined since 1978 in this laboratory. The most commonly observed tumor was the tubular adenoma. Others recorded were tubular adenocarcinoma, papillary cystadenoma, papillary cystadenocarcinoma, a Sertoli's cell tumor, benign and malignant granulosa cell tumors, benign thecal cell tumors, and classical mesotheliomas of ovarian origin. A system of classification and nomenclature of the ovarian tumors from this group is advanced.     

Light microscopical and ultrastructural characterization of black howler monkey (Alouatta caraya) ovarian follicles

The present study describes the morphological characteristics of black howler monkey (Alouatta caraya) ovarian follicles. One ovary of an adult healthy black howler monkey was collected and processed for light and electron microscopy. Primordial, primary, secondary, tertiary and pre-ovulatory follicles were evaluated for their morphometrical aspects. The ovary of black howler monkey presented a distinct conformation with a uniform distribution of the follicles mostly in the peripheric cortex. This black howler monkey ovary presented a total of 59 921 ovarian follicles. From this amount, 71.1% were classified as primordial, 18.9% as primary, 8.1% as secondary, 1.4% as tertiary and 0.5% as pre-ovulatory follicles. From all these developmental stages, the mean diameters of follicles, oocytes, oocytes nuclei and the mean number of granulosa cells are described. Moreover, primordial, primary and secondary follicles have been observed by electron microscopy.     

Equine nonneoplastic abnormal ovary in a draft mare with high serum anti-Müllerian hormone: a case study

We performed a standing hand-assisted laparoscopic ovariectomy in a draft mare that presented with high serum anti-Müllerian hormone (AMH) level and had an enlarged single cystic ovary. Histopathological examination revealed no tumor cell proliferation in the ovary, but the presence of a large ovarian cyst was confirmed. In the diagnosis of abnormal ovaries in mares, a comprehensive assessment should be performed, including the monitoring of ovarian morphology and biomarkers over time, to determine the disease prognosis and treatment plan. The case of this mare with a nonneoplastic abnormal ovary and increased serum AMH level was rare. We suggest that standing hand-assisted laparoscopic ovariectomy is useful for the removal of large ovaries in draft mares.     

孕鼠暴露双酚A对仔鼠存活率、生殖激素及相关基因的影响

为了研究孕鼠在孕期暴露双酚A（bisphenol A,BPA）对仔鼠生殖激素及相关基因的影响,试验将40只昆明孕鼠随机分为A、B、C、D共4组,每组10只。其中A组为对照组,饲喂普通鼠粮;B、C、D组孕鼠整个妊娠期（妊娠1 d至分娩）分别按每只鼠每天50、500、2 500 mg/kg体重给予BPA,待孕鼠自然分娩,观察记录仔鼠死亡情况。至仔鼠性成熟（56日龄）剖杀仔鼠,摘取睾丸或卵巢称重并计算脏器指数,HE染色观察卵巢或睾丸组织结构的变化,ELISA试剂盒分析仔鼠血清睾酮（T）、促卵泡素（FSH）及雌二醇（E₂）水平,免疫组织化学方法检测仔鼠睾丸或卵巢Bax、Bcl-2蛋白的表达,实时荧光定量PCR检测仔鼠睾丸StAR、CYP11a或卵巢AMH、Kitlg mRNA表达。结果显示,孕鼠暴露BPA极显著增加了仔鼠死亡率（P<0.01）,显著降低了仔鼠睾丸重（P<0.05）。ELISA检测结果表明,孕鼠暴露BPA极显著降低了仔鼠T（♂）及FSH（♀）含量（P<0.01）,极显著升高了仔鼠（♀） E₂水平（P<0.01）。HE染色结果显示,随BPA剂量增加,仔鼠睾丸组织损伤严重,间质细胞减少;卵巢组织结构随BPA剂量增大,空泡逐渐增多,黄体颗粒数量逐渐减少。免疫组化结果显示,孕鼠暴露BPA增加了仔鼠睾丸或卵巢组织中Bax阳性蛋白表达,减少了Bcl-2阳性蛋白表达,显著降低了雄性仔鼠StAR mRNA表达量（P<0.05）;B、D组雄性仔鼠CYP11a mRNA表达量极显著降低（P<0.01）,而C组CYP11a mRNA表达量极显著升高（P<0.01）;C、D组雌鼠Kitlg mRNA表达极显著降低（P<0.01）,AMH mRNA表达量显著升高（P<0.05）。本试验结果表明,孕鼠妊娠期暴露不同剂量BPA增加了仔鼠死亡率,扰乱了生殖激素平衡和睾丸/卵巢中相关凋亡蛋白及生殖基因表达。     

Amelanotic melanocytic tumors of the pinna in six F344 rats.

Spontaneous amelanotic melanocytic tumors of the pinna were found in six females of 960 male and 960 female albino (F344/DuCrj) rats which had been used in three different 24-month chronic toxicity studies. The age when the pinnal tumors were detected ranged from 37 to 59 weeks. The tumors were located unilaterally in the pinna and observed as subcutaneous spherical to irregular, solid white masses measuring 7 to 25 mm in diameter. The pinnal tumors were histologically classified into spindle cell and pleomorphic cell types. The spindle cell type was observed in four rats and composed of fusiform cells arranged in interlacing bundles. The pleomorphic cell type was observed in the remaining two rats and composed of pleomorphic large cells arranged in sheets. One tumor of the latter type metastasized to the submaxillary lymph node and lung. Melanin pigments were not demonstrated in any of the tumors. In immunohistochemistry, nuclei and cytoplasm of tumor cells in all the tumors were slightly positive for S-100 protein. Ultrastructurally, tumor cells contained a considerable number of premelanosomes in the cytoplasm. Desmosomes were occasionally observed between the cell membranes of the adjacent tumor cells. No distinct basal lamina was seen around tumor cells.     

Comparison of the Distribution of PGP9.5 and NPY in Cryptorchidism and Testicular Tumors in Dogs

The purpose of this study was to analyze the distribution and expression of peptidergic neurotransmitters protein gene product 9.5 (PGP9.5) and neuropeptide Y (NPY) in cryptorchidism and testicular tumors of dogs,compare them with normal testicular tissues of the same age,and provide reference for clinical diagnosis of malignant transformation in testicular tumors of dogs.HE staing,Masson trichrome staining,Gomori silver staining and toluidine blue staining were used to observe the tissue characteristics of reticular fibers,collagen fibers and mast cells.Immunohistochemical SP method and immunofluorescence combined with IPP were used to analyze the expression and localization of PGP9.5 and NPY in tissues.The results showed that the seminiferous epithelium of normal dog testis was composed of 4-7 layers of spermatogenic cells and Sertoli cells,and the distribution of collagen fibers and reticular fibers in interstitial tissue was sparse.The thickness of collagen fibers in the basement membrane of cryptorchidism seminiferous tubules increased,the nucleus of Sertoli concentrated at the base of seminiferous tubules,and the interstitial reticular fibers increased.The tissue structure of testicular tumor was unclear,collagen fibers and reticular fibers were irregularly distributed,and mast cells increased significantly compared with normal and cryptorchid groups.The immunofluorescence results showed that PGP9.5 was moderately positive in Leydig cells of normal testis,no significant expression in spermatogenic cells,strongly positive in Leydig cells and spermatogenic cells of cryptorchidism,and occasional expression in testicular tumors.NPY was occasionally expressed in normal testicular Leydig cells,but not in spermatogenic cells,strong positive expression in Leydig cells and seminiferous epithelium of cryptorchidism,high density and strong positive expression in interstitial vessels,and no obvious expression in testicular tumors.Immunohistochemical statistics showed that the expression of PGP9.5 and NPY in testicular tumor tissue were extremely significantly lower than that in normal group (P<0.01),while the expression of PGP9.5 and NPY in cryptorchidism group were significantly or extremely significantly increased (P<0.05;P<0.01).Therefore,the expression of PGP9.5 and NPY in cryptorchidism of dogs was increased suggesting that the cryptorchidism of dogs had a tendency to develop into a tumor,and was related to the degree of malignant transformation of tumor.     

犬隐睾及睾丸肿瘤中PGP9.5和NPY的分布比较

试验旨在分析肽能神经递质蛋白基因产物9.5（protein gene product 9.5,PGP9.5）和神经肽Y（neuropeptide Y,NPY）在犬隐睾及睾丸肿瘤中的分布和表达,并与同年龄正常睾丸组织进行比较,为认识犬睾丸肿瘤恶变临床诊断提供参考。应用HE染色、Masson三色染色、Gomori银浸染、甲苯胺蓝染色观察各组织中网状纤维、胶原纤维及肥大细胞等组织特征,采用免疫组织化学SP法及免疫荧光法结合IPP统计分析PGP9.5和NPY在组织中的表达及定位。结果显示,正常犬睾丸生精上皮由4~7层生精细胞及Sertoli细胞构成,间质组织胶原纤维和网状纤维分布稀疏。隐睾生精小管基底膜胶原纤维厚度增加,Sertoli细胞核浓缩位于生精小管基底,间质网状纤维增多。睾丸肿瘤组织结构不清晰,胶原纤维和网状纤维无规则分布,肥大细胞较正常组及隐睾组显著增多。免疫荧光定位表明,PGP9.5在正常睾丸Leydig细胞中呈中等阳性表达,生精细胞中无明显表达;隐睾Leydig细胞及生精细胞中呈强阳性表达;睾丸肿瘤中偶有表达。NPY在正常睾丸Leydig细胞中偶见阳性表达,生精细胞中无表达;隐睾Leydig细胞及生精上皮中无表达,间质小血管管壁呈高密度强阳性表达;睾丸肿瘤组织中无明显表达。免疫组化统计表明,睾丸肿瘤组织中PGP9.5和NPY较正常组极显著降低（P<0.01）,隐睾组PGP9.5和NPY表达显著或极显著增加（P<0.05;P<0.01）。因此,犬隐睾时PGP9.5及NPY的表达增高,提示犬隐睾时已有发展为肿瘤的趋势,且与肿瘤恶变程度相关。     

Effects of Maternal Exposure to Bisphenol A on Survival Rate,Reproductive Hormones and Relative Genes of Offspring Mice

This study was aimed to investigate the reproductive toxicity of bisphenol A (BPA) exposed to the mother on the offsprings mice. Forty pregnant Kunming mice were randomly divided into 4 groups, i.e. groups A, B, C and D with 10 mice in each group. Group A was the control group and the mice received conventional feeds, mice in groups B, C and D were given 50,500 and 2 500 mg/kg BW BPA in feedstuffs during the whole gestation period (from 1 d to parturition), respectively. The death rates of the offsprings were calculated every week. The offspring mice were sacrificed at 56 days of age (at puberty). The morphology of ovary and testicular tissues were observed with hematoxylin-eosin (HE) staining. The levels of estradiol (E₂), follicle stimulating hormone (FSH), testosterone (T) in mice serum were detected with ELISA Kit. The protein levels of Bax and Bcl-2 in ovary or testicular tissues were detected with immunohistochemistry, and the StAR,CYP11a mRNA levels in testicular tissues, the AMH, Kitlg mRNA levels in ovary were measured using Real-time PCR. The results showed that exposure of BPA to the mother extremely significantly increased the mortality (P<0.01),and significantly reduced the testicular weight of offspring mice (P<0.05). Maternal exposure to BPA extremely significantly reduced the levels of T (♂) and FSH(♀) (P<0.01),and extremely significantly elevated E₂ (♀) level in offspring mice (P<0.01). BPA exposure damaged the testicular with less leydig cells and ovarian tissues with more vacuoles and less corpus granules in offspring mice. Immunohistochemistry results revealed that maternal exposure to BPA increased the Bax protein level and decreased the Bcl-2 protein level of testicular and ovary tissues in offspring mice. BPA significantly reduced the StAR mRNA expression in male offsprings (P<0.05). However, the mRNA level of CYP11a in groups B and D extremely significantly decreased while group C showed an significant elevation in male offsprings (P<0.01). The expression levels of Kitlg mRNA in groups C and D were decreased extremely significantly in female offsprings (P<0.01), the AMH mRNA expression in groups C and D increased significantly (P<0.05). The conclusion indicated that pregnant mice exposed to different doses of BPA had harmful effects on survival rate in offspring mice, and impact the reproductive hormones, proteins and genes expression.     

The pathology of devil facial tumor disease (DFTD) in Tasmanian Devils (Sarcophilus harrisii)

A disfiguring and debilitating neoplastic condition known as devil facial tumor disease (DFTD) has been discovered in wild Tasmanian Devils (Sarcophilus harrisii) across 51% of its natural range, with population declines of up to 80% in some areas (C. Hawkins, personal communication). Between 2001 and 2004, 91 cases were examined. The tumors presented as large, solid, soft tissue masses usually with flattened, centrally ulcerated, and exudative surfaces. They were typically multicentric, appearing first in the oral, face, or neck regions. Histologically, the tumors were composed of circumscribed to infiltrative nodular aggregates of round to spindle-shaped cells, often within a pseudocapsule and divided into lobules by delicate fibrous septae. They were locally aggressive and metastasized in 65% of cases. There was minimal cytologic differentiation among the tumor cell population under light and electron microscopic examination. The results indicate DFTD to be an undifferentiated soft tissue neoplasm.     

Ultrasonographic features of testicular neoplasia in dogs: 16 cases (1980-1988)

For 16 dogs with testicular neoplasia (n = 19 tumors), ultrasonography was performed to determine whether a correlation exists between ultrasonographic features of testicular tumors and cell type. The echogenicity of the tumors varied depending on the size of the tumor and whether the tumor had focal or diffuse distribution within the testis. The ultrasonographic characteristics of Sertoli cell tumors were variable, with no predominant pattern. This variation may be related to tumor size, because 6 of 7 Sertoli cell tumors were greater than 5 cm in diameter. Focal seminomas and interstitial cell tumors less than 3 cm in diameter had hypoechoic texture. Focal seminomas and interstitial cell tumors greater than 3 cm in diameter had mixed echogenicity. Tumors of multiple cell types were greater than 5 cm in diameter and had mixed echogenicity. In valuable breeding dogs with a small (less than 3 cm) focal intrascrotal lesion, testicular ultrasonography would be of benefit for localization of the mass to the testis or epididymis for subsequent biopsy. In dogs with intraabdominal neoplastic testes, ultrasonography may be of benefit in determining intra-abdominal metastases and invasion of contiguous structures.     

Granulosa-theca cell tumor associated with an ovulation fossa and normal ovarian stroma in a mare.

A granulosa-theca cell tumor was found in an ovary that had an ovulation fossa and normal ovarian tissue. The ovary was removed from a mare with a history of ovarian enlargement and behavioral changes. The affected ovary had a multicystic appearance on ultrasonographic examination performed before surgery, and an ovulation fossa was not palpable on examination per rectum. However, during surgery, the affected ovary was found to be within normal size limits, with an enlargement on 1 pole, and to contain an ovulation fossa. Atrophy of the infundibulum of the affected ovary helped to confirm the diagnosis of granulosa-theca cell tumor, and the ovary was removed. The mare's testosterone concentrations were normal. Granulosa-theca cell tumors are usually associated with a spherical ovary, attributable to ablation of the ovulation fossa, with no normal ovarian tissue present.     

Morphometric Studies on the Testis of Korean Ring-necked Pheasant (<Emphasis Type="Italic">Phasianus colchicus karpowi</Emphasis>) during the Breeding and Non-breeding Seasons

The purpose of this study was to obtain detailed quantitative information on all cell types in the testis interstitium of Korean ring-necked pheasants and to combine these data with changes in the steroidogenic function of the testis during the breeding and non-breeding seasons. For animals collected during the breeding season, their testis weights, sperm production, serum testosterone levels and leuteinizing hormone (LH)-stimulated testosterone secretion were significantly (p < 0.01) increased compared to the non-breeding season. Testes of the pheasants during the non-breeding season displayed a 98% reduction in testis volume that was associated with a decrease in the absolute volume of seminiferous tubules (98% reduction), tubular lumen (100%), interstitium (90%), blood vessels (84%), lymphatic spaces (97%), Leydig cells (79%), mesenchymal cells (51%) and myoid cells (61%) compared to the breeding season. The numbers of Leydig cells, mesenchymal cells and myoid cells per testis in the breeding season were much higher than in the non-breeding season. Although the mean volume of a Leydig cell was 74% lower in the non-breeding season, the mean volumes of myoid and mesenchymal cells remained unchanged. These results demonstrate that there are striking differences in the testicular structure of the Korean ring-necked pheasant during the breeding and non-breeding seasons. Every structural parameter of the Leydig cell was positively correlated with both testosterone serum levels and LH-stimulated testosterone secretion. The correlation of changes in hormonal status with the morphometric alterations of Leydig cells suggests that the Korean-ring necked pheasant may be used as a model to study structure-function relationships in the avian testis.     

玉米赤霉烯酮与脱氧雪腐镰刀菌烯醇联合染毒对雄性小鼠生殖毒性的影响及茶多酚的缓解作用

本试验旨在研究茶多酚对玉米赤霉烯酮(ZEN)与脱氧雪腐镰刀菌烯醇(DON)联合染毒小鼠生殖毒性损伤的缓解作用。选取8周龄雄性昆明小鼠75只[体重(40±5)g],随机分成5组,每组分别灌服生理盐水(对照组)、200 mg/kg茶多酚(Ⅰ组)、1 mg/kg ZEN+0.5 mg/kg DON(Ⅱ组)、100 mg/kg茶多酚+1 mg/kg ZEN+0.5 mg/kg DON(Ⅲ组)和200 mg/kg茶多酚+1 mg/kg ZEN+0.5 mg/kg DON(Ⅳ组),每组15只。试验期28 d。在试验第1、14、28天,从每组随机抽取5只小鼠称重采样,检测小鼠精子质量、睾丸脏器系数、睾丸组织标志酶活性、血清性激素含量及睾丸组织氧化与抗氧化指标,并制作睾丸组织切片。结果表明:1)与对照组相比,Ⅱ组小鼠睾丸脏器系数、精子活率及数量、血清性激素含量显著降低(P<0.05),睾丸组织碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、γ-谷氨酰转移酶(γ-GGT)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性显著降低(P<0.05);精子畸形率、血清丙二醛(MAD)含量显著上升(P<0.05)。添加茶多酚后,小鼠的睾丸脏器系数、精子活率及数量、血清性激素含量、睾丸组织AKP、LDH、γ-GGT、CAT、SOD及GSH-Px活性显著上升(P<0.05),精子畸形率、血清MAD含量显著降低(P<0.05)。随着时间的推移,与第1天相比,Ⅰ组小鼠睾丸组织GSH-Px活性显著升高(P<0.05),Ⅱ组小鼠睾丸脏器系数、精子质量、睾丸组织标志酶活性、血清性激素含量、睾丸组织氧化与抗氧化指标均发生显著变化(P<0.05),Ⅲ组小鼠睾丸组织CAT及GSH-Px活性显著降低(P<0.05),Ⅳ组小鼠睾丸组织CAT活性显著降低(P<0.05)。2)睾丸组织病理切片显示染毒组小鼠睾丸生精小管基膜不完整,细胞排列紊乱,数量减少,体积缩小,细胞之间空泡化,管腔内精子数量减少,经茶多酚保护后得到改善。综上,茶多酚能有效改善ZEN与DON联合染毒小鼠精子质量与睾丸组织内标志酶活性,提高血清性激素含量及睾丸组织抗氧化功能,显著降低生殖毒性。