十氯酮污染土壤上根茎作物收割部分的污染状况

A bacterial strain, Arthrobacter oxydans (B4), capable of degrading benzo[a]pyrene (BaP) in water body, was isolated from a polycyclic aromatic hydrocarbons-contaminated site. Effects of different factors, such as reaction time, pH value, temperature and organic nutrients, on BaP biodegradation by the strain B4 were studied. After 5 d treatment, the concentration of BaP in mineral salts medium was reduced to 0.318 mg L-1 , compared to the initial concentration of 1.000 mg L-1 . There was a process of acid formation during the degradation with pH falling from initial 7.01 to 4.61 at 5 d, so keeping the water body under slightly alkaline condition was propitious to BaP degradation. Strain B4 efficiently degraded BaP at 20 to 37 ℃ with addition of organic nutrients. The biodegradation and transformation of BaP mainly occurred on cell surfaces, and extracellular secretions played an important role in these processes. Fourier transform infrared spectroscopy and gas chromatograph-mass spectrometer analyses of metabolites showed that ring cleavage occurred in the BaP degradation process and the resulting metabolically utilizable substrates were generated as sole carbon sources for B4 growth. Furthermore, mineralization extent of metabolites was verified by determining the total organic carbon and inorganic carbon in the degradation system.     

我国几种土壤中铁锰结核的元素组成和地球化学特点

The objective of this research was to isolate a dichlorvos （2,2-dichlorovinyl dimethyl phosphate）-degrading strain of Ochrobactrum sp., and determine its effectiveness in remediation of a dichlorvos-contaminated soil. A dichlorvos-degrading bacterium （strain DDV-1） was successfully isolated and identified as an Ochrobactrum sp. based on its 16S rDNA sequence analysis. Strain DDV-1 was able to utilize dichlorvos as a sole carbon source, and the optimal pH and temperature for its cell growth and degradation were 7.0 and 30 ℃, respectively. Also, the growth and degradation of strain DDV-1 showed the same response to dissolved oxygen. In addition, the soil degradation test indicated that in soil spiked with 100 mg L^-1 or 500 mg L^-1 dichlorvos and inoculated with 0.5% or 1.0% （v/v） strain DDV-1, complete degradation of dichlorvos could be achieved in 24 h. The present study showed that strain DDV-1 was a fast dichlorvos-degrading bacterium in soil. However, further research will be needed to clarify the degradation pathway and the properties of the key enzymes involved in its biodegradation.     

从土壤中分离获得的五氯硝基苯降解菌株降解五氯硝基苯特性研究

A bacterial strain,pcnb-21,capable of degrading pentachloronitrobenzene(PCNB) under aerobic and anoxic conditions,was isolated from a long-term PCNB-polluted soil by an enrichment culture technique and identified as Labrys portucalensis based upon its morphological,physiological and biochemical properties,as well as 16S rRNA gene sequence analysis.Effects of different factors,such as temperature and pH,on PCNB biodegradation were studied.Strain pcnb-21 efficiently degraded PCNB at temperatures from 20 to 30 ℃ and initial pH values from 4 to 7,which might be the first time that a Labrys strain was found capable of efficiently degrading PCNB.The degradation of PCNB was affected by oxygen,and the degradation decreased with increasing aeration.Exogenous electron donors such as glucose,lactic acid and succinic acid promoted the biodegradation of PCNB,while electron acceptors such as sodium nitrite,sodium sulfate,sodium nitrate and sodium sulfate inhibited PCNB biodegradation.The degradation of PCNB in sterile and non-sterile soils by a green fluorescent protein(GFP)-labeled strain,pcnb-21-gfp,was also studied.Cells of pcnb-21-gfp efficiently degraded 100 mg kg -1 PCNB in sterile and non-sterile soils and could not be detected after 42 days.Strain pcnb-21 might be useful in bioremediating PCNB-polluted soils and environment.     

一株芽孢杆菌属的苄嘧磺隆降解菌的分离鉴定和降解研究

The objectives of this study were to isolate a bensulfuron-methyl (BSM)-degrading strain of Bacillus spp. and to evaluate its effectiveness in remediation of a BSM-contaminated soil. A BSM-degrading bacterium, strain L1, was successfully isolated in this study. Strain L1 was identified as Bacillus megaterium based on its morphological, physiological, and biochemical properties, G+C content, phylogenetic similarity of 16S rDNA, and fatty acid compositions. Two experiments were used to examine BSM degradation by strain L1. When BSM was used as a sole carbon source in a mineral salt medium, the average degradation rate of BSM by strain L1 was 12.8%, which suggested that the strain was able to utilize BSM as a sole carbon and energy source. In addition, supplement of yeast extract (200 mg L-1) significantly (P ≤ 0.01) accelerated the degradation of BSM by strain L1. Almost complete degradation (97.7%) of BSM could be achieved in 84 h with addition of yeast extract. In addition, when a sterile soil was supplemented with BSM (50 mg L-l), BSM degradation rate was 94.3% in 42 d, indicating the potential of using microbes for the remediation of BSM-contaminated soils in fields.     

一种新的提高树苗盐容量的移植方法

The influence of quinclorac (3,7-dichloroquinoline-8-carboxylic acid) on enzyme activities in flooded paddy soils was assessed under laboratory conditions. The enzymes differed markedly in their response to quinclorac. Quinclorac inhibited proteinase, hydrogen peroxidase, phosphorylase, and urease activities. The higher the concentration of quinclorac applied, the more significant the inhibition to these observed activities with a longer time required to recover to the level of the control. However, soils supplemented with quinclorac were nonpersistent for proteinase~ phosphorylase and urease as opposed to soils without quinclorac. Dehydrogenase activity was also sensitive to quinclorac. Three soil samples with concentrations of quinclorac higher than 1 μg g^-1 soil declined to less than 20% of that in the control. However, the highest dehydrogenase activity (up to 3.28-fold) was detected in soils with 2 μg g^-1 soil quinclorac on the 25th day after treatment. Quinclorac had a relatively mild effect on saccharase activity at the concentrations used in this experiment and a stimulatory one on soil respiration when added to soil at normal field concentrations. Nonetheless it was inhibited at higher concentrations in paddy soils. Quinclorac is still relatively safe to the soil ecosystem when applied at a normal concentration (0.67 μg g^-1 dried soil) but may have some effects on soil enzymes at higher concentrations.     

Isolation and Characterization of a Bensulfuron-Methyl-Degrading Strain L1 of Bacillus

The objectives of this study were to isolate a bensulfuron-methyl (BSM)-degrading strain of Bacillus spp. and to eval-uate its effectiveness in remediation of a BSM-contaminated soil. A BSM-degrading bacterium, strain L1, was successfully isolated in this study. Strain L1 was identified as Bacillus megaterium based on its morphological, physiological, and biochemical properties, G+C content, phylogenetic similarity of 16S rDNA, and fatty acid composition. Two experiments were used to examine BSM degradation by strain L1. When BSM was used as a sole carbon source in a mineral salt medium, the average degradation rate of BSM by strain L1 was 12.8%, which suggested that the strain was able to utilize BSM as a sole carbon and energy source. Supplement of yeast extract (200 mg L-1 ) significantly (P ≤ 0.01) accelerated the degradation of BSM by strain L1. Almost complete degradation (97.7%) of BSM could be achieved in 84 h with addition of yeast extract. In addition, in a sterile soil with 50 mg L-1 BSM, BSM degradation rate by strain L1 was 94.3% in 42 d, indicating the potential of using microbes for the remediation of BSM-contaminated soils in fields.     

Optimization and modeling of glyphosate biodegradation by a novel Comamonas odontotermitis P2 through response surface methodology

Glyphosate is an important organophosphonate herbicide used to eliminate grasses and herbaceous plants in many vegetation management situations.Its extensive use is causing environmental pollution,and consequently,there is a need to remove it from the environment using an eco-friendly and cost-effective method.As a step to address this problem,a novel bacterial strain Comamonas odontotermitis P2,capable to utilize glyphosate as a carbon(C) and/or phosphorus(P) source,was isolated from a glyphostate-contaminated field soil in Australia and characterized.Response surface methodology(RSM)employing a 23 full factorial central composite design was used to optimize glyphosate degradation by C.odontotermitis P2 under various culture conditions.The strain C.odontotermitis P2 was proficient in degrading 1.5 g L~(-1) glyphosate completely within 104 h.The optimal conditions for the degradation of glyphosate were found to be pH 7.4,29.9℃,and an inoculum density of 0.54 g L~(-1),resulting in a maximum degradation of 90%.Sequencing of glyphosate oxidoreductase(GOX) and C-P lyase(phnJ) genes from C.odontotermitis P2 revealed 99% and 93% identities to already reported bacterial GOX and phnJ genes,respectively.The presence of these two genes in C.odontotermitis indicates its potential to degrade glyphosate through GOX and C-P lyase metabolic pathways.This study demonstrates the potential of C.odontotermitis P2 for efficient degradation of glyphosate,which can be exploited for remediation of glyphosate.     

除草剂莠去津和灭草松单用和混用在土壤中的降解

The application of a mixture of bentazone （3-isopropyl-1H-2,1,3-benzothiadiazin-4（3H）-one-2,2-dioxide） and atrazine （6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine） is a practical approach to enhance the herbicidal effect. Laboratory incubation experiments were performed to study the degradation of bentazone and atrazine applied in combination and individually in maize rhizosphere and non-rhizosphere soils. After a lag phase, the degradation of each individual herbicide in the non-autoclaved soil could be adequately described using a first-order kinetic equation. During a 30-d incubation, in the autoclaved rhizosphere soil, bentazone and atrazine did not noticeably degrade, but in the non-autoclaved soil, they rapidly degraded in both non-rhizosphere and rhizosphere soils with half-lives of 19.9 and 20.2 d for bentazone and 29.1 and 25.7 d for atrazine, respectively. The rhizosphere effect significantly enhanced the degradation of atrazine, but had no significant effect on bentazone. These results indicated that biological degradation accounted for the degradation of both herbicides in the soil. When compared with the degradation of the herbicide applied alone, the degradation rates of the herbicides applied in combination in the soils were lower and the lag phase increased. With the addition of a surfactant, Tween-20, a reduced lag phase of degradation was observed for both herbicides applied in combination. The degradation rate of bentazone accelerated, whereas that of atrazine remained nearly unchanged. Thus, when these two herbicides were used simultaneously, their persistence in the soil was generally prolonged, and the environmental contamination potential increased.     

百菌清降解菌株H4的分离与表征及其修复污染土壤的潜能研究

A chlorothalonil(CTN)-degrading bacterial strain H4 was isolated in this study from a contaminated soil by continuous enrichment culture to identify its characteristics and to investigate its potential for remediation of CTN in contaminated soil. Based on the morphological, physiological and biochemical tests and 16 S r DNA sequence analysis, the strain was identified as Stenotrophomonas sp. After liquid culture for 7 d, 82.2% of CTN was removed by strain H4. The isolate could degrade CTN over a broad range of temperatures and p H values, and the optimum conditions for H4 degradation were p H 7.0 and 30℃. Reintroduction of the bacteria into artificially contaminated soil resulted in substantial removal of CTN( 50%) after incubation for 14 d. Soil samples treated by H4 showed significant increases(P 0.05) in soil dehydrogenase activity, soil polyphenol oxidase activity, average well-color development obtained by the Biolog Eco plate TM assay and Shannon-Weaver index, compared with the control. Strain H4 might be a promising candidate for application in the bioremediation of CTN-contaminated soils.     

明尼苏达州中北部沙漠地区灌水种植土豆的土壤中都尔(或: 异丙甲草胺)和嗪草酮的迁移与降解

Field studies were conducted to determine the dissipation and movement of metribuzin and metolachlor applied at conventional rates to a Verndale sandy loam (Udic Argiboroll) in north-central Minnesota under irrigated potato production in two years. The rapid dissipation of both metribuzin and metolachlor was found during the initial 10 to 15 days in both years, and more than 70% of the applied herbicide dissipated during this period. From 10 to 15 days after application up to the end of growing season in both years, the levels of both herbicides decreased slowly with time. Metolachlor dissipated at a slower rate than metribuzin in surface soil and could carry over to the next cropping season. Metribuzin and metolachlor were detected in only 6 and 1 of 154 soil samples in the first year and in 3 and 4 of 225 soil samples in the second year, taken from 15 to 75 cm, respectively. Fifty to 67% of water samples from suction samplers at 135-cm depth contained detectable levels (>0.4 μg L^-1) of herbicides in both years. Under laboratory conditions degradation of both herbicides was much slower than their dissipation in field. Therefore, it appeared that leaching might be an important dissipation pathway for metribuzin and metolachlor under irrigated potato production.     

Tillage effect on organic carbon in a purple paddy soil

The distribution and storage of soil organic carbon （SOC） based on a long-term experiment with various tillage systems were studied in a paddy soil derived from purple soil in Chongqing, China. Organic carbon storage in the 0-20 and 0-40 cm soil layers under different tillage systems were in an order： ridge tillage with rice-rape rotation （RT-rr） 〉 conventional tillage with rice only （CT-r） 〉 ridge tillage with rice only （RT-r） 〉 conventional tillage with rice-rape rotation （CT-rr）. The RT-rr system had significantly higher levels of soil organic carbon in the 0-40 cm topsoil, while the proportion of the total remaining organic carbon in the total soil organic carbon in the 0-10 cm layer was greatest in the RT-rr system. This was the reason why the RT-rr system enhanced soil organic carbon storage. These showed that tillage system type was crucial for carbon storage. Carbon levels in soil humus and crop-yield results showed that the RT-rr system enhanced soil fertility and crop productivity. Adoption of this tillage system would be beneficial both for environmental protection and economic development.     

耕作方式对土壤水分入渗、有机碳含量及土壤结构的影响

为探明不同耕作方式对土壤剖面结构、水分入渗过程等的作用机理,采集田间长期定位耕作措施(常规耕作、免耕、深松)试验中的原状土柱(0~100 cm)及0~10 cm、10~20 cm、…、90~100 cm环刀样、原状土及混合土样,通过室内模拟试验进行了0~100 cm土层土壤入渗过程和饱和导水率的测定,分析了不同土层的土壤有机碳含量、土壤结构特征及相互关系。结果表明:从土柱顶部开始供水(恒定水头)到水分全部入渗到土柱底部的时间为:常规耕作免耕深松;土柱土壤入渗速率和累积入渗量为:深松免耕常规耕作;土柱累积蒸发量为:常规耕作免耕深松。土壤的饱和导水率表现为:0~10 cm和50~60 cm土层,免耕深松常规耕作;20~50 cm和60~100 cm土层,深松免耕常规耕作。随土层的加深,0.25 mm水稳性团聚体含量和土壤有机碳含量均表现为先增加(10~20 cm)再降低的趋势。在0~40 cm土层和80~100 cm土层,均以深松处理0.25 mm水稳性团聚体含量最高。在60 cm以上土层,土壤有机碳含量表现为:免耕深松常规耕作,而60 cm土层以下土壤有机碳显著降低,均低于4 g·kg?1,且在70 cm以下土层,常规耕作免耕深松。综上,耕作措施能够改变土壤有机碳含量,改善土壤结构,促进土壤蓄水保墒;深松更利于水分就地入渗,而免耕则更利于有机碳的提升和水分的储存,其作用深度在0~60 cm土层。     

秸秆还田对汉中盆地稻田土壤有机碳组分、碳储量及水稻产量的影响

为探索稻麦或稻油轮作制下,小麦、油菜秸秆还田对汉中盆地稻田土壤碳库组分的变化,设置小麦秸秆不还田(WSN)、小麦秸秆常规还田(WS)、小麦秸秆促腐还田(WSM)、油菜秸秆不还田(RSN)、油菜秸秆常规还田(RS)、油菜秸秆促腐还田(RSM),共6个处理,通过大田试验研究了不同秸秆类型及还田方式对稻田0—5,5—10,10—15,15—20,20—25cm 5个土壤层次中的土壤容重、总有机碳(TOC)、活性有机碳(LOC)、活性有机碳效率(ACL)、碳储量(SCS)、碳库管理指数(CPMI)及水稻产量的影响。结果表明:秸秆还田显著降低0—15cm土层容重,对15—25cm并未产生显著影响。与不还田相比,秸秆还田明显增加了各层次土壤有机碳库指标含量,但TOC和LOC含量均随土壤深度的增加而减少,两者在0—15cm土层含量较高,具有明显的表层富集现象;与不还田相比,小麦及油菜秸秆还田后可明显增加稻田0—25cm土层中的土壤碳储量(SCS),增幅可达21.9%~23.5%和1.7%~6.7%。不同土层中的LOC、ACL、CPIM对秸秆类型的响应不同,具体表现为小麦秸秆还田(WS、WSM)对0—15cm土层具有显著促进作用,而油菜秸秆还田(RS、RSM)对15—25cm土层中的有显著促进作用。产量方面,秸秆促腐还田模式下(WSM、RSM)水稻产量最高,常规还田模式(WSN、RSN)次之,而不还田时产量最低。相关分析显示0—10cm土壤活性有机碳有效率与水稻产量显著相关。秸秆还田是提高汉中盆地稻田土壤有机碳和产量较为有效的农田管理措施。两种轮作模式下,小麦秸秆全量旋耕还田更有利于固持稻田土壤有机碳和增加水稻产量增加。     

免耕对土壤团聚体特征以及有机碳储量的影响

以实施7年的中国科学院禹城综合试验站冬小麦夏玉米轮作免耕长期定位试验场为对象,研究免耕条件下土壤水稳性团聚体和有机碳储量的变化,为进一步评价免耕措施对黄淮海平原土壤结构和质量的影响提供科学依据。设置免耕(NT)、免耕秸秆不还田(NTRR)、常规耕作(CT)3种处理,分析土壤表层(0~20 cm)及深层(20~60 cm)水稳性团聚体分布特征、土壤有机碳以及团聚体有机碳的变化和相互关系。研究结果表明:由于减少了对土壤的破坏以及增加了秸秆还田和有机肥的施用,与常规耕作相比,NT和NTRR可提高表层土壤有机碳含量和储量、水稳性团聚体平均重量直径(MWD)和几何平均直径(GMD),以及大团聚体有机碳的含量和储量。其中,秸秆覆盖比施用有机肥对表层土壤有机碳储量和0.25~2 mm团聚体有机碳储量的提高具有更显著的作用。与表层不同,深层土壤有机碳和大团聚体有机碳的含量和储量表现为NT相似文献   

长期垄作免耕对稻田土壤有机碳剖面分布的影响

以稻田免耕长期定位试验为平台,研究长期垄作免耕对稻田土壤有机碳剖面分布的影响。结果表明,垄作免耕(中稻)、垄作免耕(稻油)、常规平作(中稻)和水旱轮作(稻油)4种耕作处理实施20年后,稻田0-60cm土体中各土层有机碳含量最高值和最低值分别出现在垄作免耕(稻油)和水旱轮作(稻油)中,且水旱轮作(稻油)中各土层有机碳含量均显著低于其他耕作处理;垄作免耕(稻油)中0-10cm和40-60cm土层有机碳含量与垄作免耕(中稻)、常规平作(中稻)之间差异不显著,但20-40cm土层有机碳含量则显著高于其他耕作处理(P<0.05),可见同传统耕作相比,长期垄作免耕(稻油)稻田的增碳优势主要体现在20-40cm土层。不同耕作处理连续实施20年后,稻田0-60cm土体有机碳密度的高低顺序为垄作免耕(稻油)>垄作免耕(中稻)>常规平作(中稻)>水旱轮作(稻油),且处理间差异显著(P<0.05)。垄作免耕15～20年期间,稻田0-10cm表层土壤有机碳储量基本稳定,但20-40cm土层有机碳储量仍有增加,其中垄作免耕(稻油)增加最为明显,表明20-40cm土层碳累积是长期垄作免耕下稻田发挥增碳功能的重要机制。     

长期施肥棕壤团聚体分布及其碳氮含量变化

【目的】探究玉米-玉米-大豆轮作体系不同施肥处理对土壤团聚体分布及其有机碳、全氮的影响,以期深入了解施肥对土壤培肥、改善土壤结构的机制。【方法】选取不施肥（CK）,化肥（NPK）,低量有机肥（M1）,低量有机肥与化肥配施（M1NPK）,高量有机肥（M2）,高量有机肥与化肥配施（M2NPK）6个处理。采集棕壤37年长期定位试验微区不同施肥处理的0-20 cm和20-40 cm土样,分析其水稳性团聚体（ 1 mm、1~0.5 mm、0.5~0.25 mm、0.25~0.053 mm及 0.053 mm）分布及其有机碳、全氮分配特征。【结果】棕壤长期施肥对团聚体分布及其碳氮的影响0-20 cm大于20-40 cm,随土层深度的增加,有机碳（SOC）、全氮（TN）含量减少。各处理团聚体及碳、氮在团聚体中的分配主要在黏粉粒中（40%以上）。与CK相比,NPK处理显著提高了黏粉粒的含量,降低大团聚体与微团聚体含量,显著增加黏粉粒储碳比例;M1、M2处理显著增加 1 mm团聚体数量及其SOC含量,显著增加 0.25 mm各粒级团聚体的储碳比例,且M2处理显著高于M1处理;M1NPK、M2NPK处理也显著增加 1 mm团聚体数量及其SOC含量,M1NPK与M2NPK处理在NPK处理的基础上依次增加0.5~0.25 mm（M1NPK）、1~0.5 mm及 1 mm团聚体的储碳比例,M2NPK处理 0.25 mm团聚体储碳比例最高,土壤团聚体全氮的变化趋势与有机碳类似。【结论】棕壤连续有机无机配合施用可显著增加土壤大团聚体数量、SOC、TN含量及其储碳、氮比例,是提高土壤质量、改善土壤结构的有效施肥措施。     

保护性耕作下土壤团聚体组成及其有机碳分布特征

依据吉林省德惠市田间定位试验（始于2001年）,对玉米-大豆轮作和玉米连作模式下秋翻（MP）、垄作（RT）和免耕（NT）3种耕作方式的机械稳定性团聚体和水稳性团聚体粒级分布、水稳性团聚体有机碳含量及团聚体稳定性进行了研究。结果表明,3种耕作方式下,〉0.25 mm机械稳定性团聚体含量均在70%以上,最高可达93.29%,各粒级含量在两个土层中表现规律性不强。水稳性团聚体含量均在20%以上,最高可达35.5%,且表层高于底层。与干筛法测定的团聚体相比,〉0.25 mm团聚体含量明显减少,最大减少幅度为58.76%。两个土层中玉米-大豆轮作和玉米连作下的机械稳定性团聚体与水稳性团聚体对耕作处理的响应表现出一定的相似性,即RT〉NT〉MP。水稳性团聚体有机碳含量随粒径的减小而增大,3种耕作方式下有机碳含量表现为NT〉RT〉MP,表层高于底层,且玉米-大豆轮作高于玉米连作。比较3种耕作方式,垄作更有利于团聚体的形成和稳定,且玉米-大豆轮作好于玉米连作。     

Soil organic carbon and nutrient content in aggregate‐size fractions of a subtropical rice soil under variable tillage

The effects of tillage on soil organic carbon (SOC) and nutrient content of soil aggregates can vary spatially and temporally, and for different soil types and cropping systems. We assessed SOC and nutrient levels within water‐stable aggregates in ridges with no tillage (RNT) and also under conventional tillage (CT) for a subtropical rice soil in order to determine relationships between tillage, cation concentrations and soil organic matter. Surface soil (0–15 cm) was fractionated into aggregate sizes (>4.76 mm, 4.76–2.00 mm, 2.00–1.00 mm, 1.00–0.25 mm, 0.25–0.053 mm, <0.053 mm) under two tillage regimes. Tillage significantly reduced the proportion of macroaggregate fractions (>2.00 mm) and thus aggregate stability was reduced by 35% compared with RNT, indicating that tillage practices led to soil structural change for this subtropical soil. The patterns in SOC, total N, exchangeable Ca²⁺, Mg²⁺ and total exchangeable bases (TEB) were similar between tillage regimes, but concentrations were significantly higher under RNT than CT. This suggests that RNT in subtropical rice soils may be a better way to enhance soil productivity and improve soil C sequestration potential than CT. The highest SOC was in the 1.00–0.25 mm fraction (35.7 and 30.4 mg/kg for RNT and CT, respectively), while the lowest SOC was in microaggregate (<0.025 mm) and silt + clay (<0.053 mm) fractions (19.5 and 15.7 mg/kg for RNT and CT, respectively). Tillage did not influence the patterns in SOC across aggregates but did change the aggregate‐size distribution, indicating that tillage affected soil fertility primarily by changing soil structure.     

免耕和秸秆还田对东北黑土区土壤团聚体组成及有机碳 含量的影响

为解决东北黑土区因不合理耕作导致的土壤结构性状变差及有机碳含量下降的问题,该研究于2015年开始,在黑龙江省哈尔滨市东北农业大学向阳试验基地开展。设置免耕+秸秆还田（NTS）、免耕（NT）、翻耕+秸秆还田（CTS）、翻耕（CT）4种处理,于2018、2019年采集土样,研究免耕措施及秸秆还田对东北薄层黑土区0～10、>10～20 cm土壤团聚体稳定性、土壤有机碳含量、各粒径团聚体内有机碳含量的影响。结果表明：2018和2019年0～10、>10～20 cm土层NTS处理>5 mm水稳性团聚体百分比含量及平均重量直径显著高于其他3种处理,NTS及NT处理土壤有机碳含量显著高于CTS及CT处理（P?<0.05）,4种处理各粒径水稳性团聚体有机碳含量峰值总体出现在1～2 mm处,NTS及NT处理>5、2～5、1～2 mm有机碳贡献率整体高于CTS及CT处理。研究表明,免耕与秸秆还田有利于薄层黑土坡耕地耕层土壤团聚体稳定性的提高和各粒级下团聚体有机碳的积累,与其他3种处理相比,免耕+秸秆还田效果更佳。     

不同耕作模式下麦田土壤温室气体排放和小麦产量

  【目的】  研究不同耕作模式对麦田土壤温室气体排放和小麦产量的影响,以期为实现小麦生产中固碳减排、绿色高产提供参考。  【方法】  供试小麦品种为‘济麦22’。本研究基于2007年的耕作模式田间定位试验,于2020—2021年小麦生长季选择4种耕作模式,即常年翻耕 (P)、常年旋耕 (R)、常年少免耕 (S)和隔两年深松+少免耕 (SS)。采集0—45 cm土层土壤样品,测定不同耕作模式下直径>0.25 mm的土壤团聚体、土壤有机碳和土壤微生物量碳含量,利用静态暗箱—气相色谱法测定温室气体排放通量,成熟期测定籽粒产量及产量构成因素。  【结果】  SS处理0—15 cm土层直径>0.25 mm的土壤团聚体含量与S处理无显著差异,显著高于P和R处理,15—45 cm土层显著高于其他处理;0—45 cm土层土壤有机碳含量和土壤微生物量碳含量最高;小麦生长季温室气体全球变暖潜力SS处理较S处理增加了7.9%,较P和R处理分别降低了12.2%和7.3%;SS处理温室气体排放强度较P、R和S处理分别减少了28.6%、28.6%和16.7%。在成熟期,SS处理的千粒重较P、R和S处理分别提高了4.7%、8.7%和9.6%,籽粒产量较P、R和S处理分别增加了7.1%、14.2%和19.4%。  【结论】  隔两年深松+少免耕 (SS) 处理增加了0—45 cm土层直径>0.25 mm的土壤团聚体含量,提高了土壤有机碳和微生物量碳含量,降低了温室气体排放强度,并获得小麦高产。综上所述,隔两年深松+少免耕 (SS) 处理是兼顾产量和环境效益的最佳耕作模式。