Biochemical changes during the development of cork spot of apples

Biochemical changes occurring during the development of cork spot of York Imperial were investigated. At the first visible sign of the disorder, the rate of ethylene production increases in the affected tissue. Respiration also increases, acetate being the major respiratory substrate rather than glucose. Protein synthesis, pectin synthesis, and the movement of inorganic ions into the tissue follow. During the time the chemical changes are taking place in the tissue, abnormal cell division is initiated, packing the newly-formed cells into the intercellular spaces. At the final stage of development, the tissue becomes brown and appears as a firm brown spot in the flesh of the apple. Cork spot is somewhat different from bitter pit in that the spots appear early in the season, the affected tissue is deeper in the flesh, and firmer. The chemical changes discovered so far in both disorders, however, appear to be similar. We consider the abnormal chemical changes that occur in both disorders to be common to diseases and injuries and not specific for either cork spot or bitter pit.

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Zusammenfassung Die Untersuchungen erstreckten sich auf biochemische Veränderungen, die während der Entwicklung von cork spot bei Äpfeln der Sorte York Imperial auftreten. Beim ersten sichtbaren Anzeichen der Störung steigt die Rate der Äthylenbildung im befallenen Gewebe an. Auch die Atmung nimmt zu, wobei Acetat das grössere Atmungsprodukt darstellt als Glucose. Protein- und Pektin-Synthese sowie Bewegung anorganischer Ionen in das Gewebe folgen. Während der Zeit chemischer Veränderungen im Gewebe beginnt eine abnorme Zellteilung, indem die neugebildeten Zellen in die interzellularen Zwischenräume eingebaut werden. Im Endstadium der Entwicklung wird das Gewebe braun und erscheint als fester Fleck im Fleisch des Apfels. Cork spot unterscheidet sich etwas von der Stippigkeit dadurch, dass die Flecken früher in der Saison erscheinen, sich die befallenen Gewebe tiefer im Fleisch befinden und fester sind. Die chemischen Veränderungen beider Störungen scheinen sich — soweit sie bekannt sind — zu ähneln. Wir glauben, dass die chemischen Veränderungen, die bei beiden Störungen auftreten, allgemein für Krankheiten und Verletzungen zutreffen und nicht entweder für Stippigkeit oder cork spot spezifisch sind.

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Resume Quelques changements biochimiques se produisant pendant le développement du cork spot chez la York Imperial ont été étudiés.Au premier signe visible de désordre, la vitesse de production d'éthylène augmente dans le tissu atteint. La respiration augmente également; l'acétate devient le substrat principal de la respiration, plutôt que le glucose. Une synthèse de protéines, de pectine, ainsi que des déplacements d'ions inorganiques dans le tissu se produisent ensuite.Pendant que les changements biochimiques ont lieu, une division cellulaire anormale est initiée, et les cellules nouvellement formées s'accumulent dans les espaces intercellulaires.Au stade final de développement, le tissu devient brun et apparaît comme une tache brune et ferme, dans la chair de la pomme. Le cork spot diffère quelque peu du bitter pit par le fait que les taches apparaissent tôt dans la saison, le tissu malade étant localisé plus profondément dans la chair, et plus ferme. Les modifications chimiques trouvées dans les deux maladies paraissent cependant être similaires. Nous considérons ces changements chimiques comme propres aux maladies et blessures en général et comme non spécifiques, ni du cork spot, ni du bitter pit en particulier.

     

Microbiological evaluation of tofu and tempeh during processing and storage

Tofu samples consisting of Fresh Tofu, Herb Tofu, Tofu Paste, Fried Tofu, Tofu Burger, Smoked Tofu and different types of herbs used for making Herb Tofu were analysed for their microbiological load. Tempeh samples consisted of cook water, inoculated beans and fresh tempeh. Fresh Tofu and Herb Tofu had high bacterial counts (10⁵ cfu/g). Tofu Burger and Smoked Tofu had counts around 10⁴ cfu/g. Tofu Paste and Fried Tofu had low counts (<10² cfu/g). Cutting board, knife and frequent hand contact with product were the major sources of contamination for tofu. Herbs used for making Herb Tofu were highly contaminated (>10⁵ cfu/g). Cook water and cooked beans for tempeh production had lower counts (10⁴ cfu/g). Initial count of fresh tempeh was high (about 10⁸ cfu/g). Most products had counts of >10⁶ cfu/g after 7 days of cold storage. The microbiological quality of the product may improve if producers avoid unnecessary contamination during processing.     

Production,nutritional profiles and storability of ‘aadun’ and ‘kokoro’: Two corn products

Aadun and kokoro were produced from maize grains by processes involving roasting, kneading, spicing, and frying. Aadun with a moisture content (MC) of 12.5%, pH 5.3 had the following composition: crude protein, 7.49%; fat, 28.5%; total carbohydrate, 60.0%; ash, 3.0% and energy value, 3,600 kcal/kg whilst kokoro with a MC of 16% and pH 5.1 had, respectively: 6.42%; 22.9%; 66.6%; 2.84% and 3,550 kcal/kg. Whilst aadun and kokoro stored under ambient conditions (28±2°C; 75–80% RH) became mouldy within 7 days, samples treated with 1% butyl paraben did not develop off-colour and mouldiness.     

Reversed phase HPLC analysis of α- and β-carotene from selected raw and cooked vegetables

Traditional AOAC colorimetric procedures for carotenoid analysis are known to lack specificity and accuracy. Newer HPLC methods provide the investigator with a more precise tool for carotenoid quantification in foods and tissues. In the present studies, reverse phase HPLC was utilized to evaluate the - and -carotene content in raw and cooked leaves of lettuce, spinach and winged bean as well as in the carrot root. The vegetables were boiled or steamed and the true retention of - and -carotene in the cooked products was determined. Boiling for 30 minutes resulted in a 53 and 40% loss of -carotene from lettuce and carrots, respectively. Full retention or even an increase in -carotene content in boiled winged bean leaves and spinach was noted. Steaming resulted in very good retention of - and - carotene in all vegetables (83–139% retention). Thus, although cooking procedures (especially boiling) may result in oxidative loss of carotenoids in some vegetables, heat treatment increases the chemical extractability of - and -carotene in others. The presence of carotenoproteins in some vegetables may effect the heat stability of extractability of - and - carotene.     

Protein quality and digestibility of new high-lysine barley varieties in growing rats

Four new high-lysine barley mutants, the variety Lysimax, with the high-lysine genelys3a and the mutants mother variety Sultan were grown in a field trial in 1993 at Risø, Denmark. Mutants 609, 1242, 1385 and 1405 yielded in the range of 89 to 98 percent and cv Lysimax yielded 102 percent of cv Sultan (100 percent). One-thousand kernel weights for the mutants were in the range of 87 to 97 percent and cv Lysimax 83 percent of cv Sultan (100 percent). Protein contents of the mutants were slightly higher, in the range of 13.2 to 13.6 percent, than of cv Sultan (13.1 percent) and Lysimax which had a protein content of 12.6 percent. Fat content was higher in Lysimax and in the mutants except for mutant 1385 than in cv Sultan while dietary fibre contents of the barleys were similar. The levels of -glucans and starch were usually lower in Lysimax and in the mutants. The highest lysine levels: 4.6, 4.0 and 3.7 g/16 g N occurred in cv Lysimax and mutants 609 and 1405 compared to 3.3, 3.3 and 3.2 for cv Sultan and mutants 1242 and 1385, respectively. Mutants 609 and 1405 and cv Lysimax also had higher levels of threonine, histidine and valine. The increased lysine contents resulted in large, at most 20 percent, increases in biological value; 88.8, 81.7 and 78.3 percent for cv Lysimax and mutants 609 and 1405 compared to 74.2 percent for cv Sultan. True protein digestibilities and energy digestibilities were slightly lower in Lysimax than in Sultan, 5.3 and 4.3 percentage units, respectively. It is concluded that the development of high-lysine barley varieties is very beneficial for meeting the requirements of indispensable amino acids for humans and monogastric animals. In addition, nitrogen excretion into the environment is drastically reduced due to the higher biological values of the mutants.     

Zusammenhänge zwischen den für die Blaufleckigkeit der Kartoffelknolle verantwortlichen Inhaltsstoffen

Zusammenfassung Mit der Faktorenanlyse nach der Hauptachsenmethode und der aufbauenden multiplen Regressionsanalyse, unter Einbeziehung von 56 Einflußgrößen, konnte gezeigt werden, daß die Blaufleckigkeit von Kartoffelknollen im wesentlichen vom Gehalt an Trockensubstanz, -Aminobuttersäure, Lysin, Isochlorogensäure und den Mineralstoffen Ca und K abhängt. Eine kausale Erklärung des gesamten Komplexes Blaufleckigkeit ist allerdings zum gegenwärtigen Zeitpunkt noch nicht möglich.

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Relationships between black spot of potatoes and several constituents of the tuber. With a factorial analysis and a multiple regression analysis could be shown that from 56 investigated constituents of the potato tuber only the dry matter content, aminobutyric acid, lysine, isochlorogenic acid, and potassium and calcium content influence the appearance of black spots.A causal explanation of the black spot phenomenon at present is not possible. Further investigations are necessary.

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Résumé Relations entre la tâche noire des pommes de terre et divers constituants du tubercule.L'analyse factorielle, l'analyse de la régression multiple ont pu démontrer que sur 56 constituants déterminés dans le tubercule de pomme de terre seuls l'acide aminobutyrique, la lysine, l'acide isochlorogénique, le taux de potassium et de calcium agissent sur la formation de la tâche noire.Une explication causale du phénomène tâche noire n'est pas encore possible pour l'instant. De nouvelles recherches sont nécessaires.

     

The areas and the exploitation of the Matricaria chamomilla L. in Jugoslavia

Conclusion The annual production ofMatricaria in the Banat varies from 10–600 tons of dried flowers. This great oscillation in the harvest is the function of a continental climate with great extremes in atmospheric-water and temperature during March and May.The greatest danger for theMatricaria during spring is dryness and the hot south-east winds Koava.The purest population ofMatricaria, the richest and most compact is found in the loam and salt-soda soil slatinas on the east of the river Theiss. Only in the Jugoslav Banat there are about 150.000 hectares of slatinas, which are covered withMatricaria every spring.TheMatricaria does not exist in the quicksands of the Banat.The pH (optimal) forMatricaria seems to be 8, but it can support a pH up to 9 under the condition that there is not much sodium chloride and a sufficient amount of rain during the period of March–May.The wind is the most important propagator of the seeds of theMatricaria in the Banat. The role played by man and cattle is also important.Besides the slatinas the areas most important for theMatricaria are the plains along streams and big rivers, in general in the quaterner.Only in the Banat the exploitation of theMatricaria flower is industrialized. The town of Kikinda is the oldest and best known export centre.With 8 figures     

On determination of chitin content in mushrooms

Nitrogen compounds constitute the greatest proportion of the dry weight of mushrooms. These nitrogen compounds are partly protein and partly non-protein. A modified Heneberg-Stohman's method was used to separate non-protein from protein nitrogen, as well as to determine raw fiber content in mushrooms, since the methods used previously for this purpose are time-consuming and complicated. Elemental analysis of crude fiber was performed to confirm the uniformity of isolated products. The content of total crude fiber (fungin) and the proportion of chitin and cellulose in it were determined. The chitin content of 15 species of field grown edible mushrooms was in the range of 1.87–6.93% of total dry mass and 34.51–67.72% of isolated fungin.     

Influence of grain quality,heat, and processing time on the reduction of aflatoxin B1 levels in ‘tuwo’ and ‘ogi’: Two cereal-based products

During the production of tuwo from laboratory-contaminated corn (AFB₁:150 mcg/kg) and sorghum (AFB₁:87.5 mcg/kg) grains, reductions in the aflatoxin-B₁ levels of pastes boiled for 30 min and 60 min were found to be 68.0% and 80.8%, respectively. In the preparation of ogi from contaminated corn and sorghum grains, reductions of about 72.5% and 71.4%, respectively, were obtained after fermentation at ambient conditions. Reconstitution of ogi paste into a porridge (akamu) considerably reduced the AFB level.     

Evaluation of turmeric accessions for quality

One hundred and eighty four accessions ofCurcuma were analysed for curing percentage (dry recovery), essential oil, oleoresin and curcumin contents. Curing percentage varied from 13.5 to 32.4. The cultivar Konni had the maximum percentage of oleoresin (19.2). The volatile oil content was more inC. aromatica than inC. domestica. Curcumin content varied from 2.3% in cultivar Hahim to 10.9% in cultivar Edapalayam. However, curcumin content was comparatively lower in six exotic types as well as in 14 related species. The importance of quality aspects with respect to breeding is discussed.Contribution No. 493 of Central Plantation Crops Research Institute, Kasaragod-670 124, Kerala, India.     

Neue Gemüsesorten für den Qualitätsanbau für die Verarbeitung

Zusammenfassung Bei einigen für die verarbeitende Industrie wichtigen Arten und zwar bei Buschbohnen, Erbsen, Spinat, Möhren, Freilandgurken, Rote Rüben, Knollensellerie und Puffbohnen werden die in den letzten Jahren neu auf dem Markt erschienenen Sorten besprochen, soweit sie sich für den vorgenannten Zweck eignen. Unter dem Begriff Qualität werden die von Schuphan definierten Begriffe äußere Beschaffenheit, Gebrauchswert und Biologischer Wert verstanden. Auf Wunsch wird der Krankheitsresistenz ganz besondere Beachtung geschenkt und die gegen die wichtigsten Krankheiten resistenten Sorten bei der jeweiligen Art werden entsprechend hervorgehoben. So werden die gegen Colletotrichum lind. sowie gegen Virus 1 resistenten Sorten bei Buschbohnen, die gegen falschen Mehltau widerstandsfähigen Spinatsorten und bei Freilandgurken, die gegen Krätze und Mosaik toleranten sowie bitterstoffreien Sorten vorgestellt. Bei Erbsen werden die für die Gefrier- und Naßkonservierung geeigneten Sorten, insbesondere im Hinblick auf Korngröße (Sortierung) und Kornfärbung herausgestellt. Ähnliches gilt für die Puffbohne. Bei Möhren wird vor allem der Carotingehalt der neuen Sorten dem der alten gegenübergestellt, während bei Sellerie die weißkochenden oder die bezüglich der Knollenform und bei Rote Rüben, die hinsichtlich der intensiven Fleischfärbung und der Rübenform verbesserten Sorten besprochen werden.

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New vegetable varieties for technological preservation

New varieties of French beans, peas, broad beans, cucumbers, spinach, carrots, celery and red beets for preservation are discussed.The term quality implies shape, agricultural value and biological value of a variety as defined by Schuphan. Special attention has been given to the varieties' resistance to disease. Mentioned are varieties of French beans resistant toColletotrichum lind. and common bean mosaic, pea varieties resistant toFusarium oxysp., cucumber varieties resistant toCladosporium cucum. and mosaic and free of bitter matter, and spinach varieties resistant toPeronospora spin.Characteristics of new pea and broad bean varieties suitabele for canning and freezing are discussed with regard to size and colour of grains. The carotin content of new carrot varieties is compared with that of standard varieties. Further, new celery and beet varieties with superior shape and improved colouring of roots are discussed.

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Resume Parmi les légumes servant de matière première aux industries de mise en conserve, beaucoup ont fait récemment l'objet de sélections nouvelles, conduisant à l'introduction sur le marché de variétés améliorées: haricots verts, pois, épinards, carottes, cornichons de pleine terre, betteraves rouges, céleris-raves, fèves; nous décrirons ces variétés, dans la mesure de leur aptitude à la conserverie. La qualité que nous envisagons est celle définie par Schuphan; portant sur les critères de conformation d'aptitude à l'utilisation, et de valeur nutritionelle. Une attention particulière est consacrée, sur demande, à la résistance aux maladies; dans chaque genre, nous décrivons les cultivars résistants.Ainsi, nous décrivons les variétés de haricots verts résistant à l'anthracnose, au virus 1, les variétés d'épinards résistant au faux mildiou, les variétés de cornichons de pleine terre tolérantes auCladosporium cucumerinum et à la Mosaique, et qui soient dépourvues d'amertume. Nous décrivons les variétés de Pois aptes à la congélation ou à la conservation en boite, par la taillé et la couleur de la graine; nous en faisons autant pour les fèves. Les nouvelles variétés de Carottes se distinguent par leur taux de caroténoides; les nouvelles variétés de céleris restent blanches à la cuisson, et ont une forme plus commodes. Les betteraves-rouges nouvelles sont plus colorées, et de formes plus pratiques.

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Vortrag gehalten am 20. April 1971 in Geisenheim/Rhg. anläßlich der Vortragstagung der Deutschen Gesellschaft für Qualitätsforschung (Pflanzliche Nahrungsmittel) (DGQ) e.V.     

Nutritive characterization of purslane accessions as influenced by planting date

The uniqueness of purslane (Portulaca spp.) asthe richest vegetable source of omega-3 (-3)fatty acids is well documented. However, purslane hasnot been domesticated or fully evaluated for itsnutritive value. The objective of this study was todetermine the influence of planting date on chemicalcomposition of purslane accessions. Eight accessionsfrom different geographical locations were planted 12days apart, and whole plants harvested at full bloom. Chemical analysis (DM basis) of leaves showedsignificant differences among varieties for all thecharacteristics measured. Accession by planting dateinteraction influenced (p < 0.05) levels of crudeprotein, total lipids, and carbohydrate contents. Wild Greek accession had the highest, while aBeltsville (Maryland) wild type had the lowest crudeprotein content (27.1 vs 20.5%) at the secondplanting date. Crude protein, lipid and ash levelswere most influenced (p < 0.05) by planting date. Total lipids varied from 4.0–5.8% and 3.7–5.1% forthe first and second planting dates, respectively. Selected fatty acid content indicated significantly(p < 0.05) higher levels of 18: 26, and18: 33 in the Dutch Garden accession comparedwith other varieties. The Egyptian wild accession hadthe lowest level of 18: 33. The ratio of 3 to 6 acids, which ranged from 5.5 to22.3 indicated a highnutritive value of purslane compared to other oilcrops such as soybeans and perrilla. The high levelsof protein in purslane compete with those of othercommercially important vegetable crops. The studyshows that, in spite of its genetic diversity, purslaneremains one of the most abundant terrestrial vegetablesources of Omega-3 fatty acids and other essentialnutrients potentially beneficial for humans as well asanimals.     

Seed globulins of Cajanus cajan

Seed globulins ofCajanus cajan a widely cultivated legume were purified and characterised. About 78% of the seed proteins were salt soluble, out of which 61% were globulins which were further separated into three fractions. The fraction was insoluble at pH 4.7 and consisted of two subfractions. Fraction and were soluble at pH 4.7. All the fractions were characterised as glycoproteins by cesium chloride centrifugation. The proteins consisted of subunits which were not held together by covalent disulphide linkages. Amino acid analysis of the different globulin fractions showed that the fraction was comparatively rich in sulphur amino acids.     

Über die Einwirkung einiger Ätherischer Öle auf höhere Pflanzen

Zusammenfassung Im Rahmen der Untersuchungen phytodynamischer Eigenschaften ätherischer Öle sind Versuche über Wirkung von AetheroleumLavandulae, Menthae, Rosmarini, Salviae undThymi auf das Auskeimen eingeweichter und trockener Achänen des Salats(Lactuca sativa L.), sowie auf das Wachstum der Keimlinge dieser Achänen, durchgeführt worden. Ätherische Öle wurden in gelöstem oder dampfförmigem Zustand (Atmosphäre) angewendet.Festgestellt wurde, daß Atmosphäre oder Lösung schwächerer Konzentration der angewendeten ätherischen Öle fast nie inhibitorisch auf das Auskeimen der eingeweichten Achänen wirkt, aber, daß eine solche Wirkung auf trockene Achänen sehr ausgeprägt ist. Lösungen ätherischer Öle wirken schwächer als ihre Atmosphären. Atmosphären und Lösungen aller angewendeten ätherischen Öle zeigen weder eine Regelmäßigkeit in ihrer Wirkung auf das Auskeimen der Achänen, noch können Gruppen ätherischer Öle hervorgehoben werden, welche ständig ähnlich wirken.Das Wachstum der Keimlinge ist gehemmt durch die Wirkung der Lösung ätherischer Öle, bedeutender in Fällen, in denen das Auskeimen der Achänen schwächer ist. Das Längenwachstum der Keimlinge ist immer im größeren Maße als das Auskeimen gehemmt.Die inhibitorische Wirkung auf beide beobachteten Prozesse ist proportional der Konzentration der Atmosphäre oder Lösung.

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Summary In the course of examinations on phytodynamic properties of volatile oils, assays with respect to the effect of Aetherol.Lavandulae, Menthae, Rosmarini, Salviae andThymi on germination of moistened and of dry lettuce achenes(Lactuca sativa L.), as well as on growth of the germ sprouted from these achenes have been carried out. The volatile oils were applied either in form of solution or as vapour (atmosphere).It has been stated that atmospheres as well as solutions of lower concentrations of the applied volatile oils hardly ever act inhibitory on germination of moistened achenes, whilst on dry achenes it proved to be rather considerable. The effect of solutions, on germination of achenes, is less raised than that of atmospheres of volatile oils.Neither do atmospheres as well as solutions of all the applied volatile oils constantly show a certain regularity in effect on germination of achenes nor could any volatile oil group of a similarly constant effect be stated.The longitudinal growth of germs is inhibited by the effect of volatile oil solutions, however, it is stronger when a slower germination of achenes is in question. The longitudinal growth is more inhibited than the process of germination.The inhibitory effect on both processes is proportional to the concentration of the atmosphere or of the solution.

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Zakljuak U okviru ispitivanja fitodinamskog svojstva eterskih ulja vreni su pokusi djelovanja Aetheroleum Lavandulae, Menthae, Rosmarini, Salviae i Thymi na klijanje kvaenih i suhih roki salate(Lactuca sativa L.), te na rast klica razvitih iz tih roki. Eterska ulja su primijenjena u obliku otopine ili hlapljevine (atmosfere).Utvrdjeno je da atmosfere ili otopine slabijih koncentracija eterskih ulja gotovo nikad ne djeluju inhibitorno na isklijavanje kvaenih roki, dok je takvodjelovanje na suhe roke vrlo znatno. Na isklijavanje slabije djeluju otopine od atmosfera eterskih ulja. Atmosfere i otopine svih primijenjenih eterskih ulja niti stalno pokazuju neku pravilnost u djelovanju na isklijavanje roki niti se mogu uturditigrupe eterskih ulja koje bi se stalno odlikovale slinim djelovanjem.Duinski rast klica je koen djelovanjem otopina eterskih ulja, a znatniji je u sluojevima u kojima je isklijavanje roki brojnije. Duinski rast sam je u veoj mjeri koen od isklijavanja.Inhibitorno je djelovanje u oba promatrana procesa proporcionalno s koncentracijom atmosfere ili otopine.

     

Traditional production and chemical composition ofNdaleyi,a Nigerian fermented pearl millet food

Ndaleyi, a fermented, sun dried agglomerated powder produced from pearl millet or sorghum is one of the most popular foods consumed in Nigeria, mostly by the Kanuri people of Borno State. Its traditional production is described. The mean yields ofndaleyi (mainly starch),chir (millet gluten) and bran (overtail orbina) were 30.6, 22.5 and 21.5%, recpectively. A mean yield loss of 20% was observed. Chemical analysis revealed thatchir and bran have higher protein, fat and ash contents thanndaleyi. The titratable acidities (as percent lactic acid) ofndaleyi andchir were 0.9 and 0.3, while their pH values were 3.3 and 3.5, respectively.     

Chemical changes during the fermentation of African locust-bean (Parkia filicoidea Welw) seeds for production of ‘Daddawa’

African locust-bean (Parkia filicoidea Welw) seeds were allowed to ferment under natural conditions and the chemical changes occurring during the fermentation were studied. Chemical analysis of fermented and unfermented beans showed a marked decrease in the total sugar content; crude and true protein levels all increased. The results of the chemical analyses indicate that fermentation resulted in protein enrichment of the fermented locust bean (daddaw). Fermentation also imparts a characteristic flavour and odour to the beans.     

Effect of alkaline treatment (‘akanwu’) and supplementary value of corn or crayfish on the protein quality of breadfruit (Treculia Africana)

The effect of treatment and supplementary value of corn (C) or crayfish (CR) on the protein quality of breadfruit (Treculia Africana) flours were studied in eighteen young rats. The 7030 or 701515 (Protein basis) combinations of breadfruit flours and corn or crayfish or both provided 1.6g N/100 g diet for the 35 day study. The addition of akanwu to the cooking water reduced cooking time and crude protein and saved fuel. The addition of akanwu and replacement of CR with C was not beneficial as judged by the parameters tested except for the wt. gain and PER. On the other hand, when crayfish was the only source of supplementary protein (30%) to breadfruit cooked without akanwu there were increases in all parameters tested over those with added akanwu except for the N intake, wt. gain, and PER.These results appear to suggest that addition of akanwu to TA was detrimental to its protein utilization and that TA appears to be an economic source of N in areas where it is a staple. Based on the results of this study, one would suggest that the use of akanwu as a tenderizer should be seriously looked into before further use.     

Inhibition of α-amylase purified fromOctopus vulgaris Lam. by albumin inhibitors from wheat flour

Octopus -amylase has been purified to homogeneity by single-step affinity chromatography on Sepharose-bound wheat albumin inhibitors. Two electrophoretically distinguishable isoamylases are obtained, both consisting of a single polypeptide chain with molecular weight 45,000. Octopus -amylase is more effectively inhibited by a monomeric than by a dimeric protein inhibitor from wheat. The inhibition is dependent on pH, temperature, and time of preincubation. Properties are compared with those of other animal -amylases.     

Nutrient and antinutrient composition of three varieties ofPiper species

The proximate composition of three varieties ofPiper guineense (Odusa — Ibibio/Efik) viz. Uyat Odusa (cultivated and peppery), Eting-keni Ikot (wild forest variety) and Eting-keni mben inyang (wild, riverine variety), were determined using available standard methods. Also determined were mineral, antinutrient and ascorbate levels. The cultivated pepperic variety had the highest content of crude protein and moisture (18.9% and 97% respectively) while the wild, riverine variety had the highest content of ether extract, carbohydrate and calories (7.79%, 63.38% and 398 cals respectively). The cultivated variety had appreciable amounts of phosphorus (1.12 mg/100 g), potassium (1.2 mg/100 g), sodium (0.24 mg/100 g), zinc (0.18 mg/100 g), and copper (0.18 mg/100 g) while the forest variety contained more of calcium (12.38 mg/100 g), magnesium (1.21 mg/100 g) and iron (0.85 mg/100 g). The wild riverine variety appeared to have the least mineral content but had the highest ascorbate level of 173.4 mg/100 g. Of four antinutrients assayed, the cultivated pepperic one had the least quantities while the forest variety was highest in hydrocyanic acid (85.8 mg/100 g) and glucosinolates (0.20 mg/100 g). The wild riverine variety had the highest level of total oxalate (165.0 mg/100 g). These quantities are however far below documented toxic levels.     

Pestizidprobleme tropischer Länder

Ohne ZusammenfassungZusammenfassung des Vortrags, gehalten am 30.9.1976 anläßlich der Jahrestagung der Deutschen Gesellschaft für Qualitätsforschung (Planzliche Nahrungsmittel) in Geisenheim/Rheingau.