An assessment of the behaviors of overwintering manatees as influenced by interactions with tourists at two sites in central Florida

We used a combination of focal animal and scan samples over the course of two winter seasons to assess behavioral patterns of manatees as a function of the presence and activities of recreational swimmers and boats in and around Crystal River National Wildlife Refuge, Florida. The nature and outcome of human-manatee interactions and the frequency of harassment of manatees by swimmers (as defined by the US Fish and Wildlife Service) were also recorded. The use of protected (no-entry) sanctuaries by manatees was significantly greater when both the numbers of swimmers and boats increased, and when water temperatures were lower in surrounding areas. The time manatees spent bottom-resting and nursing decreased while the time spent milling and swimming increased when swimmers were present compared with when they were absent. Over half of the direct interactions recorded between swimmers and manatees constituted some form of harassment as defined by Refuge guidelines, and manatees were far more likely to terminate these interactions than were swimmers. We conclude that the existence of no-entry sanctuaries is very important for the conservation of manatees in the area and thought must be given to expanding the sanctuary network. Furthermore, additional efforts at enforcement and public education are needed. We address, qualitatively, several potential management options to better-secure manatees at this critical time of the year.     

Development of predictive models to explain the distribution of the West Indian manatee Trichechus manatus in tropical watercourses

Logistic regressions were used to study the relationship between habitat variables and the use of tropical watercourses by the West Indian manatee Trichechus manatus at Northeastern Costa Rica and Southern Nicaragua. Presence of manatees in watercourses was assessed through direct and reported sightings of individuals and feeding signs on aquatic vegetation. Indirect methods provided good approximations to the actual distribution that could not have been achieved through direct observations or aerial surveys. Best multivariate models showed that manatees were most present in watercourses that presented abundant aquatic vegetation, warm, and clear waters, high forest cover, and are wider than those where the species is absent. Although habitat variables that explain habitat use of manatees differed for the two sectors found within the study area, manatees preferred lagoons to other watercourses in both areas. These findings point to forest clearing on the shores as a threat for manatee conservation. Habitat variables are excellent predictors of manatee presence, and predictive models as those developed in this study can help assess potential distribution of manatees in areas where this information is lacking as well as to assist identify potential reintroduction areas.     

Manatee metabolism and its influence on distribution in Florida

The metabolic rate of three captive manatees was measured at different water and air temperatures. The animals responded metabolically to cold water (15–20°C), but not to cold air (10–20°C). Average metabolic rates in the thermal neutral zone ($\text{T}{}_1\text{? 24°}$) were 15–22% of predicted weight-specific values, and thermal conductances were 117–229% of predicted levels. Body temperatures averaged 36·4°C. The low heat production and high thermal conductance suggest that manatees are poorly adapted energetically to winter water temperatures in much of Florida. These results support behavioural observations of captive and free-living manatees which suggest that 20°C is the minimum suitable water temperature for manatees. Manatees wintering in colder waters are probably in marginal habitat and are most susceptible to cold-related mortality. Warm water refuges reduce energetic maintenance costs and probably temper the effects of occasional periods of severely cold weather, particularly in northern Florida.     

Florida manatees, Trichechus manatus latirostris, respond to approaching vessels

Florida manatees inhabit shallow coastal and estuarine waters of the southeast US, a range that brings them into frequent contact with vessels. More than 30% of documented annual mortalities are attributed to vessel collisions, and most living animals bear the scars of multiple, non-lethal encounters. To document the behavior of manatees in the presence of vessels, we recorded their movements with an overhead video system. We scored six aspects of behavior during 170 vessel approaches, and compared their behavior with 187 control segments when no boats were present. Manatees in shallow waters and at the edge of the channel responded to approaches by orienting towards the nearest deep water, a boat channel, and increasing their swimming speed. Close boat approaches and shallow water depths exacerbated these responses. Our results indicate that manatees detect and respond to approaching vessels with an apparent flight response, a response which includes movement towards deeper water. If given sufficient time, i.e., approached or passed slowly, the manatees may then be able to reach deeper water and safe depths.     

Commercial exploitation of manatees Trichechus in Brazil c. 1785–1973

All available published statistics on the exploitation of the Amazonian manatee Trichechus inunguis are collected and analysed to determine the major trends in exploitation and, if possible, their effects on the species. The manatee's role in the Amazonian economy has traiditionally been on the level of subsistnce hunting for local consumption; export of surplus manatee products has been limited and sporadic, and keeping of records has been even more so. Unknown quantities of manatee meat were shipped to the Guianas and West Indies in the 17th century, At least from the 1780s to about 1925, the only manatee product common in Amazonian commerce was mixira (fried meat packed in lard). From 1935 to 1954, manatee hides were exported to southern Brazil and elsewhere for the manufacture of heavy-duty leather. From 1954 to the legal banning of manatee hunting in 1973, manatees were again heavily exploited for meat. At no time did manatee products make up more than a small fraction of a percent of the Amazon region's trade. Numbers of animals killed per year cannot be determined with any precision, but early statistics on mixira reflect no more than 1000/year, while the peak years of hide and meat production probably required 4000–7000 manatees/year, exclusive of unrecorded subsistence hunting. Taking the latter into account, it is likely that actual mortality ran to several thousand animals per year throughout the past two centuries. Decline of meat production in the 1960s may reflect overexploitation. Evidence was also found of some exploitation for meat of West Indian manatees T. manatus in the states of Alagoas and Bahia, Brazil, the latter constituting the southernmost record of the species since the early 19th century.     

Tolerance of West Indian manatees to capture and handling

Ninety-two West Indian manatees (Trichechus manatus) have been captured in the southeastern United States from October 1975 through November 1983 with no evidence of an unusual susceptibility to capture myopathy. Of these, 53 were radio-tracked or observed in the field following capture with no evidence of delayed capture stress. Blood samples obtained at capture for 20 wild individuals displayed no elevation in biochemical variables typically seen in mammalian capture myopathy cases. Thirty-one manatees captured for rescue and rehabilitation generally did not exhibit symptoms of capture myopathy, although in one case marked elevations in creatinine phosphokinase were observed, probably related to lengthy transport. Necropsy findings for 20 terminal cases that died of various causes unrelated to their rescue or handling did not indicate the involvement of any gross or histological lesions of capture myopathy. Historical records for the capture and handling of about 150 West Indian manatees during the 1900s also suggest a tolerance to these activities. Recent observations on the probable susceptibility of dugongs (Dugong dugon) to capture stress apparently cannot be extended to West Indian manatees.     

Genetic diversity and parentage in farmer varieties of cacao (Theobroma cacao L.) from Honduras and Nicaragua as revealed by single nucleotide polymorphism (SNP) markers

Cacao (Theobroma cacao L.) is the main source for chocolate with an annual production of four million tons worldwide. This Neotropical tree crop was domesticated in Mesoamerica as far back as 3,000 years ago. Knowledge of genetic diversity and population structure in farmer varieties of cacao in the center of domestication is essential for sustainable production of fine-flavored cacao beans and contributes to in situ/on-farm conservation of farmer varieties. Based on 70 single nucleotide polymorphism markers, we analyzed 84 fine-flavored farmer varieties collected from traditional cacao farms in Honduras and Nicaragua. The study also included 31 clones from the international cacao collections to serve as references. The SNP based multilocus matching identified six synonymous groups, including 14 Criollo and two Amelonado varieties. A moderately high level of genetic diversity was observed in these farmer varieties, indicating the possibility to further explore intra-population variation and breed for fine-flavored cocoa. Multivariate analysis showed clustering of the 84 farmer accessions in five genetic groups: ancient Criollo, Amelonado, Trinitario (including Nicaragua Trinitario and Honduras Trinitario) and Upper Amazon Forastero (only one accession). The Honduras Trinitario differed from the Nicaragua Trinitario group. The clustering results largely supported the perceived classification of cacao by local farmers and researchers, which was mainly based on morphological traits. However, the well known traditional variety “Indio” in this region was identified as synonymous with Amelonado. Parentage analysis showed that the variety “Indio” (or Amelonado) contributed more to the Trinitario type farmer varieties, whereas ancient Criollo had less influence. The present study demonstrates the efficacy of using a small set of SNP makers for cacao germplasm characterization, and further depicts the diverse origins and parentage in farmer varieties from Mesoamerica. This information thus will be highly useful for conservation and utilization of cacao germplasm from this region.     

Assessing landscapes: a case study of tree and shrub diversity in the seasonally dry tropical forests of Oaxaca, Mexico and southern Honduras

Tropical dry forests, with their distinct and economically important diversity, are acknowledged conservation priorities because of alarming rates of forest conversion. Whilst it is realised that terrestrial conservation requires an understanding of landscape level patterns of diversity, forests are rarely assessed accordingly. Here we demonstrate that, in the case of the seasonally dry tropical forests of the Pacific watershed of Mesoamerica, landscape level assessment of woody diversity can inform decision making relevant to both between-landscape and within-landscape prioritisation. We report floristic surveys of dry forest landscapes in Oaxaca, Mexico and southern Honduras. It is noted that these forests are floristically similar to other seasonally dry tropical forests in the neotropics. By calculation of Genetic Heat Indices, a relative measure of the concentration of restricted range species in a sample, we determine that the conservation of the tree diversity of the coastal lowlands of Oaxaca should be prioritised over that of southern Honduras. The current conservation status of forested areas in Oaxaca is briefly described. We suggest that the greater degree of anthropogenic disturbance in southern Honduras may explain the relative lack of restricted range species there. We argue that some forest fallows can act as analogues of mature forest and therefore landscape elements other than mature forest need to be included in forest conservation assessments. We conclude that diversity sampling of any forest type should not be limited to mature forests, but extended to other elements of forested landscapes.     

Distribution and abundance of manatees trichechus manatus in the Dominican Republic

Aerial surveys and interviews were conducted in the Dominican Republic to determine the distribution and abundance of manatees. Results show that two reproducing populations range through marine habitat of northern and southwestern coastal segments. Calves were seen throughout the year, indicating continuous breeding.Illegal hunting is identified as the major source of mortality and stricter enforcement of existing laws would help to preserve the remaining population.     

Biodiversity of common bean (Phaseolus vulgaris L.) in Honduras, evidenced by morphological characterization

This paper describes findings in the characterization of 300 accessions in a collection of common beans (Phaseolus vulgaris L.) in Honduras. The plant material was collected from 1990 to 1994 (4 years before the damaging Mitch Hurricane) covering most of the Departments of the country and kept at the Escuela Agrícola Panamericana Zamorano. Thirty-two morpho-agronomical characters were evaluated and the results were grouped by classes. The studied accessions showed a marked diversity with high predominance of red and small seeds. The accessions with purple flowers had black seeds and originate mainly from the western area of the country where this type of seed is preferred. Principal Component Analysis shows that clearly defined groups do not exist. The conservation of this diversity is recommended for future propagation, breeding and the investigation of the genetic relationships and other studies.     

Distribution and status of manatees Trichechus spp. Near the mouth of the amazon river,Brazil

Interviews with hunters and collection of skulls indicate that Trichechus inunguis occurs throughout the region of the Amazon River estuaries from Amapá to the mainland of Pará, including Ilha de Marajó and islands on its Atlantic coast. T. manatus has a disjunct distribution in Brazil, apparently occurring both on the coast of Amapá north of Cabo Norte and in the Rio Mearim (Maranhão) as well as further to the southwest; it seems to have been exterminated from the Atlantic coast of Pará and is absent from the Marajó region. Continued subsistence hunting can best be controlled by destruction of illegal camboas (fence-like traps) which catch manatees at high tide. Areas deserving study are the coast and inland lakes of eastern Amapá, the only place in the world where two sirenian species might still be found in sympatry or immediate proximity, and the lower Rio Mearim in Maranhão, which may still contain a sizable population of T. manatus. Ecological studies in these areas should have particular relevance to hypotheses of sirenian evolutionary interactions, and manatee reserves should be established in both areas.     

Multi-scale system approaches in agronomic research at the landscape level

Spatial multi-scale analyses of actual land use system performance as determined by spatial yield variability reveals the need for landscape research in agronomy. Main ‘drivers’ of spatial yield variability for five different crops in Honduras, Costa Rica and Ecuador were identified. It is demonstrated how they vary with spatial scales and that landscape-related factors often play a large and significant role in when the variability in yield is determined. These results indicate that landscape experiments in agronomy are relevant. Apart from empirical analysis, spatial–temporal explicit modeling of landscape process dynamics such as water and soil redistribution within a landscape can give insight in the performance of agronomic systems within a dynamic landscape context. For a case study in the South of Spain it is demonstrated how within a landscape this type of research can determine the on- and off-site effects of water and soil redistribution in agro-ecosystems. Only after a spatially explicit multi-scale system analysis and explorative landscape process modeling is completed, relevant agronomic landscape experiments can be designed.     

Effect of different dosage and administration schedules of folic acid on blood folate levels in a population of Honduran women of reproductive age

BACKGROUND: Observational studies and clinical trials have shown conclusive evidence that periconceptional folic acid supplementation prevents up to 70 % of neural tube defects (NTD). The Honduran government wanted to implement a supplementation programme of folic acid but needed to assess the relative effects of two dosages of folic acid. OBJECTIVE: To determine the effect of two dosages of folic acid on blood folate levels in Honduran female factory workers aged 18 to 49 years. DESIGN: This was a randomized, double-blind control supplementation trial conducted in Choloma, Honduras. A total of 140 eligible women were randomly assigned to two dosage groups and followed up for 12 weeks. One group received a daily dosage of 1 mg folic acid and the other a once weekly dosage of 5 mg. Serum folate and red blood cell folate levels were determined by radioassay at baseline, 6 weeks and 12 weeks. RESULTS: Serum folate levels increased from 6.3 (se 0.2) to 14.9 (se 0.6) ng/ml (P < 0.0001) in women assigned to the 1 mg/d group and from 6.9 (se 0.3) to 10.1 (se 0.4) ng/ml (P < 0.0001) in those assigned to the 5 mg/week group. Red blood cell folate concentrations also increased significantly in both groups, albeit more slowly. Educational level, age and BMI were not associated with the changes in serum and red blood cell folate levels during the supplementation period. However, a differential effect on serum folate levels by dosage group and time was observed. CONCLUSIONS: Although both folate supplementation regimens increased serum and red blood cell folate levels significantly among the women studied, blood folate levels that are considered to be protective of NTD were reached faster with the daily dosage of 1 mg folic acid.     

Steepland resources: characteristics, stability and micromorphology

Steeplands of Central America are a major land resource, but due to population pressures they are rapidly being deforested leading to landscape instability. Microwatersheds of Southern Honduras were examined to correlate soil type, landform, slope gradient and slump potential. Soils most susceptible to slumping were moderately deep (0.5–1 m) Haplustalfs and Haplustepts. These soils occupy about 25% of the landscape, and have slope gradients of 45–90%. Soils are developed on plagioclase-rich andesitic parent material, are loam to clay loam in texture and have high base status. However, due to weathering of parent material, sand and silt fractions are dominated by quartz with smaller amounts of plagioclase, vermiculite and kaolinite. The A and B horizons have an open porphyric related distribution with plagioclase as the coarse fraction in a fine-grained groundmass. The andesitic parent rock contains abundant hydrothermally altered plagioclase phenocrysts in a single-spaced porphyric-related (c/f_5μm ratio of 4:1) distribution. Some feldspars are partially to completely altered to clay pseudomorphs, probably vermiculite. Microfabric analysis of soil and pararock did not show any striated b-fabric indicative of shear failure. However, microfabrics do confirm translocated clay into the subsoil as typic pore coatings indicating long-term landscape stability prior to deforestation.     

Effects of soil degradation and management practices on the surface water dynamics in the Talgua River Watershed in Honduras

When tropical forests are felled, subsequent land uses affect surface runoff, soil erosion, and soil compaction. In some cases, they can markedly change the hydrology of a region with disastrous effects on human life. The objective of this paper is to investigate the effect of rainfall on stream hydrology due to conversion of primary forests to agriculture. Near surface water dynamics were compared for three land uses on the steep hillsides in the Talgua River Watershed in Honduras: degraded grass‐covered field; traditional coffee plantation; and primary forest. Infiltration and surface runoff rates were measured using several methods. A clear difference was observed in hydraulic conductivity between the degraded and non‐degraded lands. The degraded grass‐covered hillslopes developed a surface restrictive layer with a low saturated hydraulic conductivity of 8 to 11 mm/hr, resulting in more frequent overland flow than traditional coffee plantation and primary forest. Soils under the latter two land‐use types maintained high infiltration capacities and readily conducted water vertically at rates of 109 and 840 mm/hr, respectively. Dye tests confirmed that the coffee plantation and primary forest both maintained well‐connected macropores through which water flowed readily. In contrast, macropores in the degraded soil profile were filled by fine soil particles. Soils in the degraded grass‐covered field also showed more compaction than soils in the coffee plantation. Copyright © 2004 John Wiley & Sons, Ltd.     

Effects of insularisation on plant species richness in the prairie-forest ecotone

Data on plants from five groups of remnant prairies and forests in the prairie-forest ecotone of the midwestern United States show that: (1) Archipelagos of small sites tend to contain more species than do single large ones of equal total area. (2) No species are excluded from small sites. (3) Small sites tend to have surprisingly many species, and large sites surprisingly few, relative to a random colonisation model. (4) ‘Rare’ species (those that occur in only one site) are found more often in small sites than a random colonisation model would predict. (5) There is no evidence for any of these sites that species number is ‘relaxing’, but all these sites have been remnants for only a short time. Result (1) is consistent with results from similar studies on other taxa and suggests that there is no automatic reason to believe that species richness is maximised by single large refuges.     

Influence of topographic and edaphic factors on vulnerability to soil degradation due to cattle grazing in humid tropical mountains in northern Honduras

Low altitude humid tropical mountains in Central America have experienced a process of livestock expansion during recent decades. However, the use of sloping areas for cattle grazing may lead to significant soil degradation and therefore we examined the influence of the slope gradient on soil degradation in pastures in a humid tropical mountainous area in northern Honduras. Understanding this relationship permits estimates of the physical carrying capacity of the soil, which in turn may help to improve livestock use within the study area. Variables examined included soil bulk density, texture, organic matter content and consistency as well as visual indicators of soil and vegetation degradation. There is a significant positive correlation between the bulk density as a proxy for soil degradation and slope gradient. Furthermore, it was found that when soils are water-saturated grazing leads to severe degradation. Together with visual indicators, these data show that paddocks with slopes less than 30% have a carrying capacity between 900 and 1900 Animal Units (AU) ha^− 1 year^− 1 and many are currently underutilized. Paddocks with slopes between 30 and 50% have a carrying capacity between 400 and 600 AU ha^− 1 year^− 1. Paddocks with slopes over 50% have the lowest carrying capacity: less than 200 AU ha^− 1 year^− 1. The latter are frequently over-used; most of them show clear signs of soil and vegetation degradation. Land use in these areas needs to change or their grazing management needs to be reorganized to adjust actual stocking rate to physical carrying capacity of the soils to prevent further degradation.     

用粒度分析法计算砒砂岩区小流域泥沙来源的探讨

小流域泥沙来源是土壤侵蚀研究的重要内容 ,目前国内外主要利用径流泥沙资料 ,采用统计分析方法来确定小流域不同地貌部位及不同侵蚀类型的产沙量。采用粒度分析法对砒砂岩不同侵蚀类型区典型小流域泥沙来源进行分析计算的结果表明 :小流域泥沙主要来自于沟谷地 ,其产沙量占小流域总产沙量的 79 39%～ 84 89% ;沟间地产沙量占15 86 %～ 2 0 16 %。     

基于侵蚀力学机制的流域土壤侵蚀模型研究—以长江三峡库区王家桥流域为例

根据王家桥流域观测资料及人工降雨实验成果,从侵蚀力学机制出发,严格区分了坡面侵蚀与流域侵蚀两个不同的概念,建立了坡面侵蚀模数和流域侵蚀模数。计算结果表明,流域内坡面侵蚀只占流域侵蚀模数的7.1%～11.7%,流域侵蚀物质主要来源于沟蚀、部分重力侵蚀。因此在王家桥流域如何开展水保工作是一个值得深思的问题。     

Effect of seed phosphorus concentration on nodulation and growth of three common bean cultivars

Abstract

An experiment was conducted in the greenhouse to evaluate the effects of seed phosphorus (P) concentration on growth, nodulation, and nitrogen (N) and P accumulation of three common bean (Phaseolus vulgaris L.) cultivars. Seeds were produced under low or high soil P levels, and soaked, or not, in 200 mM KH₂PO₄ solution. The experiment had a 3×3×2×2 factorial block design: three cultivars (ICA Col 10103, Carioca and Honduras 35), three levels of applied P (15, 30 and 45 mg P kg^?1 soil), two native seed P concentrations, and two seed soaking treatments. Plants were harvested at flowering. Soaked seeds increased the number, dry mass and P content of nodules, but did not affect plant growth. Plants originated from seeds with high native P concentration presented higher shoot dry mass and nodule number and mass at every soil P level, and were less responsive to increased soil P supply, than plants from low seed P. In plants from seeds with high P, soil P levels did not alter significantly root dry mass, while in plants from seeds with low P bean cultivars expressed wider differences in root dry mass. The genotypic variability of nodulation was influenced by soil P levels and seed P concentration. Both higher soil or seed P supply enhanced N and P accumulation in shoots. These results indicate that a high seed P concentration produces plants less dependent on soil P supply, and can enhance nodulation and N₂ fixation of common bean. Seed P supply affected the cultivar performance, and should be considered in evaluation of bean genotypes.