Water quality implications of dairy slurry applied to cut pastures in the northeast USA

Abstract. Nitrate nitrogen (NO₃-N) leaching from animal production systems in the northeast USA is a major non-point source of pollution in the Chesapeake Bay. We conducted a study to measure NO₃-N leaching from dairy slurry applied to orchardgrass ( Dactylis glomerata L., cv. Pennlate) using large drainage lysimeters to measure the direct impact of four rates of slurry (urine and faeces) N application (0, 168, 336, 672 kg N ha⁻¹ yr⁻¹) on NO₃-N leaching on three soil types. We then used experimentally-based relationships developed earlier between stocking density and NO₃-N leaching loss and leachate NO₃-N concentration to estimate the added impact of animal grazing. Nitrate N leaching losses from only dairy slurry applied at the 0, 158, 336, and 672 kg N ha⁻¹ yr⁻¹ rates were 5.85, 8.26, 8.83, and 12.1 kg N ha⁻¹ yr⁻¹, respectively with corresponding NO₃-N concentrations of 1.60, 2.30, 2.46, and 3.48 mg l⁻¹. These NO₃-N concentrations met the 10 mg l⁻¹ US EPA drinking water standard. However, when a scenario was constructed to include the effect of NO₃-N leaching caused by animal grazing, the NO₃-N drinking water standard was calculated to be exceeded.     

Short-term changes in nitrogen availability, gas fluxes (CO2, NO, N2O) and microbial biomass after tillage during pasture re-establishment in Rondônia, Brazil

Anthropogenic conversion of primary forest to pasture for cattle production is still frequent in the Amazon Basin. Practices adopted by ranchers to restore productivity to degraded pasture have the potential to alter soil N availability and N gas losses from soils. We examined short-term (35 days) effects of tillage prior to pasture re-establishment on soil N availability, CO₂, NO and N₂O fluxes and microbial biomass C and N under degraded pasture at Nova Vida ranch, Rondônia, Brazilian Amazon. We collected soil samples and measured gas fluxes in tilled and control (non tilled pasture) 12 times at equally spaced intervals during October 2001 to quantify the effect of tillage. Maximum soil NH₄⁺ and NO₃⁻ pools were 13.2 and 6.3 kg N ha⁻¹ respectively after tillage compared to 0.24 and 6.3 kg N ha⁻¹ in the control. Carbon dioxide flux ranged from 118 to 181 mg C–CO₂ m² h⁻¹ in the control (non-tilled) and from 110 to 235 mg C–CO₂ m² h⁻¹ when tilled. Microbial biomass C varied from 365 to 461 μg g⁻¹ in the control and from 248 to 535 μg g⁻¹ when tilled. The values for N₂O fluxes ranged from 1.22 to 96.9 μg N m⁻² h⁻¹ in the tilled plots with a maximum 3 days after the second tilling. Variability in NO flux in the control and when tilled was consistent with previous measures of NO emissions from pasture at Nova Vida. When tilled, the NO/N₂O ratio remained <1 after the first tilling suggesting that denitrification dominated N cycling. The effects of tilling on microbial parameters were less clear, except for a decrease in qCO₂ and an increase in microbial biomass C/N immediately after tilling. Our results suggest that restoration of degraded pastures with tillage will lead to less C matter, at least initially. Further long-term research is needed.     

Nitrous oxide and carbon dioxide emissions from grassland amended with sewage sludge

Abstract. Land disposal of sewage sludge in the UK is set to increase markedly in the next few years and much of this will be applied to grassland. Here we applied high rates of digested sludge cake (1–1.5×10³ kg total N ha⁻¹) to grassland and incorporated it prior to reseeding. Using automated chambers, nitrous oxide (N₂O) and carbon dioxide (CO₂) fluxes from the soil were monitored 2–4 times per day, for 6 months after sludge incorporation. Peaks of N₂O emission were up to 1.4 kg N ha⁻¹ d⁻¹ soon after incorporation, and thereafter were regularly detected following significant rainfalls. Gas emissions reflected diurnal temperature variations, though N₂O emissions were also strongly affected by rainfall. Although emissions decreased in the winter, temperatures below 4 °C stimulated short, sharp fluxes of both CO₂ and N₂O as temperature increased. The aggregate loss of nitrogen and carbon over the measurement period was up to 23 kg N ha⁻¹ and 5.1 t C ha⁻¹. Losses of N₂O in the sludge-amended soil were associated with good microbial conditions for N mineralization, and with high carbon and water contents. Since grassland is an important source of greenhouse gases, application of sewage sludge can be at least as significant as fertilizer in enhancing these emissions.     

Total selenium content of agricultural soils in Japan

To evaluate the selenium (Se) level in agricultural soils in Japan and to investigate its determining factors, 180 soil samples were collected from the surface layer of paddy or upland fields in Japan and their total Se contents were determined. Finely ground soil (50 mg) was wet-digested with HNO₃ and HClO₄ solution and the released Se was reduced to Se(IV). The concentration of Se(IV) was then determined by high-performance liquid chromatography with a fluorescence detector after treatment with 2,3-diaminonaphthalene and extraction with cyclohexane. The total Se content ranged from 0.05 to 2.80 mg kg⁻¹ with geometric and arithmetic means of 0.43 and 0.51 mg kg⁻¹, respectively. The overall data showed a log-normal distribution. In terms of soil type, volcanic soils and peat soils had relatively high Se content and regosols and gray lowland soils had relatively low Se content. In terms of land use, upland soils had significantly higher Se content than paddy soils. Among regions, soils in the Kanto, Tohoku, Hokkaido and Kyushu regions had relatively high content. The total Se content had a significant positive correlation with the organic carbon content ( P  < 0.01) and the equation for the estimation of total Se content with organic carbon suggested that on average approximately 48% (0.24 mg kg⁻¹) of the total Se was in inorganic forms and approximately 52% (0.25 mg kg⁻¹) was in organic forms. Soil pH, on the contrary, did not show a significant relationship with the total Se content. In conclusion, the organic matter content, in combination with volcanic materials, was the main determining factor of the total Se content of agricultural soils in Japan.     

Experimental and simulated soil mineral N dynamics for long-term tillage systems in northern France

Soil N mineralization was quantified in two long-term experiments in northern France, in which no-till (NT) and conventional tillage (CT) had been differentiated for 33 years (Site 1) and 12 years (Site 2). Both sites had the same soil type but differed in crop rotation. N mineralization kinetics were assessed in situ in bare soil in both systems for 254 days (Site 1) and 555 days (Site 2) by taking frequent measurements of water and nitrate contents from soil layers and using the LIXIM calculation model. The N mineralization potential was also determined in soil samples incubated under controlled laboratory conditions. Small or non-significant differences in water and nitrate contents between NT and CT were apparent within the soil profiles on both sites. Net mineralization did not differ significantly between sites or tillage treatments. The amount of N mineralized from August 2003 to April 2004 was 67 ± 10 kg N ha⁻¹ on Site 1 and 74 ± 5 kg N ha⁻¹ on Site 2, and 161 ± 6 kg N ha⁻¹ from August 2003 to February 2005 on Site 2. The kinetics of N mineralization versus normalized time (equivalent time at constant temperature of 15 °C and water content at field capacity) were linear during the shorter period (254 days corresponding to 120 normalized days). The slope (N mineralization rate) did not differ significantly between treatments and sites, and the average rate was 0.57 ± 0.05 kg N ha⁻¹ nd⁻¹. The kinetics were non-linear on Site 2 over the longer period (555 days corresponding to 350 normalized days). They could be fitted to an exponential model with a slope at the origin of 0.62 kg N ha⁻¹ nd⁻¹. The N mineralization kinetics obtained in laboratory incubations for 120–150 normalized days were also almost linear with no significant differences between treatments. Assuming that mineralization took place in the ploughed layer (in CT) or over the same soil mass (in NT) they were in good agreement with the kinetics determined in situ on both sites. The calculated water drainage below the sampled profile was slightly greater in NT due to lower evaporation. The calculated leached N was slightly higher in NT than CT on Site 1, but did not differ between treatments on Site 2. It is concluded that N mineralization and leaching in NT and CT were similar, despite large differences in N distribution within the soil profile and a slight difference in organic N stock.     

Measurement of ammonia volatilization loss using a dynamic chamber technique: A case study of surface-incorporated manure and ammonium sulfate in an upland field of light-colored Andosol

The present study aimed to elucidate ammonia (NH₃) volatilization loss following surface incorporation (0–15 cm mixing depth) of nitrogen (N) fertilizer in an upland field of light-colored Andosol in central Japan. A dynamic chamber technique was used to measure the NH₃ effluxes. Poultry manure, pelleted poultry manure, cattle manure, pelleted cattle manure and ammonium sulfate were used as N fertilizers for basal fertilization to a bare soil with surface incorporation. All three experiments in summer and autumn 2007 and in summer 2008 showed negligible NH₃ volatilization losses following the application of all N fertilizers with the same application rate of 120 kg N ha⁻¹ as total N; these negligible losses were primarily ascribed to chemical properties of the soil, that is, its high cation exchange capacity (283 mmol_c kg⁻¹ dry soil) and relatively low pH(H₂O) (5.9). In addition, the surface incorporation, the very small ratio of ammoniacal N to total N for the manure, and the decrease in soil pH to ≤5.5 following applications of ammonium sulfate were also advantageous to the inhibition of NH₃ volatilization loss from the field-applied N fertilizers.     

Effects of disturbance and glucose addition on nitrous oxide and carbon dioxide emissions from a paddy soil

The effects of disturbance and glucose addition on N₂O and CO₂ emissions from a paddy soil at 45% WFPS (water-filled pore space) and at 25 °C were determined. During a 45-day incubation, disturbances with and without glucose addition were imposed 0, 1, 3, and 5 times. The total amount of glucose added to soil with 1, 3, and 5 disturbances was equal (0.6% of oven-dry soil basis). Strong nitrification occurred in the paddy soil during the incubation. Disturbance alone did not influence N₂O and CO₂ emissions significantly, but disturbance with glucose addition did (P < 0.01). A flush of N₂O as well as CO₂ was always observed following disturbance with glucose addition. The discrepancy in N₂O emission between disturbance alone and disturbance with glucose addition was ascribed to the different magnitude of denitrification and/or heterotrophic nitrification. Greater cumulative emission of N₂O was observed in the treatment of three disturbance times with glucose addition (4.3 mg N kg⁻¹ soil), compared with five disturbances with glucose addition (2.5 mg N kg⁻¹ soil) and one disturbance with glucose addition (2.5 mg N kg⁻¹ soil). Cumulative CO₂ emission was significant larger in one and three disturbances with glucose addition than that five disturbance with glucose addition. Supplies of available organic C appear to be a critical factor controlling denitrification and/or heterotrophic nitrification processes and N₂O emission under relatively low moisture conditions, i.e. 45% WFPS.     

Geologic Nitrogen as a Source of Soil Acidity

The origin of highly acidic (pH<4.5) barren soils in the Klamath Mountains of northern California was examined. Soil parent material was mica schist that contained an average of 2,700 mg N kg⁻¹, which corresponds to 7.1 Mg N ha⁻¹ contained in a 10-cm thickness of bedrock. In situ soil solutions were dominated by H⁺, labile-monomeric Al³⁺ and NO₃⁻, indicating that the barren area soils were nitrogen saturated—more mineral nitrogen available than required by biota. Leaching of excess NO₃⁻ has resulted in removal of nutrient cations and soil acidification. Nitrogen release rates from organic matter free soil ranged from 0.0163 to 0.0321 mg N kg⁻¹ d⁻¹. Nitrogen release rate from fresh ground rock was 0.0465 mg N kg⁻¹ d⁻¹. This study demonstrates that geologic nitrogen may represent a large and reactive nitrogen pool that can contribute significantly to soil acidification.     

Increased predicted losses of phosphorus to surface waters from soils with high Olsen-P concentrations

Abstract. Diffuse soluble reactive P (SRP) & total P (TP) loads from over 50 major river catchments in Northern Ireland were predicted using an export coefficient modelling approach. Phosphorus export coefficients for each CORINE land cover class, derived from satellite imagery, allowed the prediction of P loads from a breakdown of the CORINE land cover classes by catchment using a GIS. This approach was validated using observed P loads calculated from flow and concentration data. Mean measured Olsen-P concentrations in the soil A-horizon were also determined on a catchment basis. Plots of P loads to the watercourse versus Olsen-P concentrations in the soil showed a breakpoint around 22 mg Olsen- P l⁻¹ for both SRP & TP data. Below Olsen-P concentrations of 22 mg l⁻¹, SRP & TP losses were essentially independent of Olsen-P at 0.28 and 0.63 kg P ha⁻¹ yr⁻¹, respectively. Above Olsen-P concentrations of 22 mg l⁻¹, there was considerable spread in the P loss data. Nevertheless, significant upward trends in SRP and TP losses to watercourses were detected with increasing Olsen-P at a rate of approximately 0.5 and 1.0 kg P ha⁻¹ yr⁻¹, for SRP and TP respectively, for each 10 mg l⁻¹ increase in Olsen-P.     

Emission of nitric oxide and nitrous oxide from soil under field and laboratory conditions

A detailed short-term (12 d) laboratory study was carried out to investigate the effects of applying animal urine, fertilizer (ammonium nitrate) and fertilizer+urine on emission of NO and N₂O from soil. A complementary 24 d field study measured the effect of fertilizer or fertilizer+sheep grazing on NO and N₂O emissions from pasture. The data generated were used to interpret the transformations responsible for the release of these gases. Application of urine to the soil (at a rate equivalent to 930 kg N ha⁻¹) increased the amount of mineral and microbial N in the soil. This was followed by increases in emissions of NO (from 0.02 to 1.76 mg NO-N m⁻² d⁻¹) and N₂O (from 15 to 330 mg N₂O-N m⁻² d⁻¹). Molar ratios of NO-N-to-N₂O-N were very low (<0.001 to 0.011) indicating that denitrification was the main process during the first 12 d after application. In the laboratory, nitrification was inhibited during the first 7 d due to an inhibitory effect of the urine, but even though nitrification was clearly underway 7–12 d after application, denitrification was still the dominant process. The fertilizer was applied at a lower rate (120 kg N ha⁻¹) than the urine. Consequently, the effect on soil mineral N was smaller. Nevertheless the fertilizer still increased NO and N₂O emission with denitrification the dominant process. The effects of fertilizer and grazing on NO and N₂O emissions was less obvious in the field compared with the laboratory and fluxes returned to background rates within 4 d. This was attributed to the rapid decline in soil mineral N in the field trial due to plant uptake and leaching, processes that did not occur in the laboratory.     

N mineralization and nitrate leaching from vegetable crop residues under field conditions: a model evaluation

Six different vegetable crop residues were incorporated in the field and N mineralization from the residues and from an unamended plot was followed over 4 months by periodically monitoring mineral N contents of the soil. The crop residues were also fractionated according to a modified Stevenson chemical fractionation. Nitrogen mineralization parameters of the first order kinetic model N(t)=N_A(1−e^−kt) were derived from the chemical fractionation data. The first order model was used in combination with a model describing the temperature dependence of N mineralization and a simple leaching model to predict N mineralization rates and nitrate redistribution after crop residue incorporation under field conditions. Comparison of predicted and measured mineral N contents in the upper soil layer (0–30 cm) before the start of leaching showed that the model was able to predict N mineralization from both soil organic matter and crop residues under field conditions. From the onset of leaching, mineral N contents were slightly overestimated in the upper layer and underestimated in the lower soil layers. Although the Burns leaching model underestimated the leaching rate, the general pattern of nitrate movement was simulated satisfactorily. Statistical analysis using the variance ratio test yielded small but significant F values, indicating that the model can still be improved. The modelling efficiency was rather high and the coefficient of residual mass very close to zero. Linear regression between measured and simulated nitrate contents over the whole profile (0–120 cm) for all samplings yielded Y=9.6+0.876X (r=0.94***) with all deviations smaller than 25 kg N ha⁻¹. Total N mineralization ranged from 48 kg N ha⁻¹ for the control plot to 136 kg N ha⁻¹ for the plots with cauliflower residues and cumulative leaching losses from 26–66 kg N ha⁻¹, with most of the mineral N left in the 60–120 cm layer. These results show that N losses by leaching in winter can be high when vegetable crop residues are incorporated, even when there is little mineral N in the soil at the time of incorporation.     

The impact of long-term cultivation and management history on the status and dynamics of cobalt in a savanna Alfisol in Nigeria

The status of cobalt (Co) in savanna soils of Nigeria is largely unknown, and a long-term experiment including inorganic fertilizer (NPK) and farmyard manure (FYM) and uncultivated land provided information on the way management affected the dynamics of Co in the soil. Total Co increased with increasing depth, whereas readily extractable Co decreased. The mean concentration of Co (5.6–7.9 mg kg⁻¹) was close to the mean value of 8 mg kg⁻¹ reported for soils worldwide, whereas the concentration of extractable Co was less than that reported in most soils. Regression analysis indicated that total Fe predicted up to 78% of the soil Co. The potentially available Co correlated strongly with pedogenic or reducible Mn oxides extracted with dithionite–citrate–bicarbonate. Mass balance calculations showed that fertilization with either NPK or FYM caused losses of between 0.8 and 1.1 g Co m⁻² after 50 years of cultivation against the uncultivated site as a reference. However, Co increased by 1.8 g m⁻² in the soil receiving FYM + NPK, suggesting that the Co of the soil was best maintained under this management probably because of incidental additions of Co in the manures. Furthermore, the positive Co balance in the FYM + NPK plot was partly enhanced by its larger contents of clay, Fe and pedogenic Mn oxides than in either the FYM or NPK plots. Clay, Fe and pedogenic oxides served as Co sinks in this particular savanna soil.     

Effects of tillage and nitrogen management on soil chemical and physical properties after 23 years of continuous sorghum

Long-term tillage and nitrogen (N) management practices can have a profound impact on soil properties and nutrient availability. A great deal of research evaluating tillage and N applications on soil chemical properties has been conducted with continuous corn (Zea Mays L.) throughout the Midwest, but not on continuous grain sorghum (Sorghum bicolor (L.) Moench). The objective of this experiment was to examine the long-term effects of tillage and nitrogen applications on soil physical and chemical properties at different depths after 23 years of continuous sorghum under no-till (NT) and conventional till (CT) (fall chisel-field cultivation prior to planting) systems. Ammonium nitrate (AN), urea, and a slow release form of urea were surface broadcast at rates of 34, 67, and 135 kg N ha⁻¹. Soil samples were taken to a depth of 15 cm and separated into 2.5 cm increments. As a result of lime applied to the soil surface, soil pH in the NT and CT plots decreased with depth, ranging from 6.9 to 5.7 in the NT plots and from 6.5 to 5.9 in the CT plots. Bray-1 extractable P and NH₄OAc extractable K was 20 and 49 mg kg⁻¹ higher, respectively, in the surface 2.5 cm of NT compared to CT. Extractable Ca was not greatly influenced by tillage but extractable Mg was higher for CT compared to NT below 2.5 cm. Organic carbon (OC) under NT was significantly higher in the surface 7.5 cm of soil compared to CT. Averaged across N rates, NT had 2.7 Mg ha⁻¹ more C than CT in the surface 7.5 cm of soil. Bulk density (Δ_b) of the CT was lower at 1.07 g cm⁻³ while Δ_b of NT plots was 1.13 g cm⁻³. This study demonstrated the effect tillage has on the distribution and concentration of certain chemical soil properties.     

Monitoring of water erosion on arable farms in England and Wales. 1990–94

Abstract. The incidence of soil water erosion was monitored in 12 erosion-susceptible arable catchments ( c . 80 fields) in England and Wales between 1990 and 1994. Factors associated with the initiation of erosion were recorded, and the extent of rills and gullies measured. Approximately 80% of the erosion events were on land cropped to winter cereals. In 30% of cases, the initiation of erosion was linked to valley floor features, which concentrated runoff. Poor crop cover, wheelings and tramlines were also assessed as contributory factors in 22%, 19% and 14% of cases, respectively. In c . 95% of cases rainfall events causing erosion were ≥10 mm day⁻¹ and c . 80% were >15 mm day⁻¹. Erosion was also associated with maximum rainfall intensities of >4 mm h⁻¹ for c . 90% of cases and >10 mm h⁻¹ for c . 20%. Mean net soil erosion rates were approximately 4 t ha⁻¹ per annum (median value 0.41 t ha⁻¹ per annum) and associated mean P losses 3.4 kg ha⁻¹.     

Soil organic carbon accumulation and carbon costs related to tillage, cropping systems and nitrogen fertilization in a subtropical Acrisol

Conservation management systems can improve soil organic matter stocks and contribute to atmospheric C mitigation. This study was carried out in a 18-year long-term experiment conducted on a subtropical Acrisol in Southern Brazil to assess the potential of tillage systems [conventional tillage (CT) and no-till (NT)], cropping systems [oat/maize (O/M), vetch/maize (V/M) and oat + vetch/maize + cowpea (OV/MC)] and N fertilization [0 kg N ha⁻¹ year⁻¹ (0 N) and 180 kg N ha⁻¹ year⁻¹ (180 N)] for mitigating atmospheric C. For that, the soil organic carbon (SOC) accumulation and the C equivalent (CE) costs of the investigated management systems were taken into account in comparison to the CT O/M 0 N used as reference system. No-till is known to produce a less oxidative environment than CT and resulted in SOC accumulation, mainly in the 0–5 cm soil layer, at rates related to the addition of crop residues, which were increased by legume cover crops and N fertilization. Considering the reference treatment, the SOC accumulation rates in the 0–20 cm layer varied from 0.09 to 0.34 Mg ha⁻¹ year⁻¹ in CT and from 0.19 to 0.65 Mg ha⁻¹ year⁻¹ in NT. However, the SOC accumulation rates peaked during the first years (5th to 9th) after the adoption of the management practices and decreased exponentially over time, indicating that conservation soil management was a short-term strategy for atmospheric C mitigation. On the other hand, when the CE costs of tillage operations were taken into account, the benefits of NT to C mitigation compared to CT were enhanced. When CE costs related to N-based fertilizers were taken into account, the increases in SOC accumulation due to N did not necessarily improve atmospheric C mitigation, although this does not diminish the agricultural and economic importance of inorganic N fertilization.     

Carbon dioxide fluxes from cyanobacteria crusted soils in the Kalahari

The surface of dryland soils is frequently characterised by a biological crust comprising of various combinations of cyanobacteria, algae, moss and lichens. In the Kalahari of Botswana, soil crusts are predominantly made up of cyanobacteria, which when moist, are capable of fixing N and C. Many cyanobacteria also produce extracellular polymeric substances (EPS) which bind soil particles together and decrease erodibility. The physical integrity and metabolic activity of soil crusts is thus critical to ecological productivity, erodibility and CO₂ fluxes in dryland regions. There are, however, few studies of the magnitude and controlling factors of soil CO₂ flux within these systems.

Our aim was to quantify in situ soil CO₂ flux during contrasting antecedent moisture conditions in the south west Kalahari of Botswana. We have designed a gas exchange chamber for field deployment coupled to a portable gas chromatograph, control and data logging instrumentation. The optical and active thermal control specifications of the chamber have been designed to permit photosynthesis and cope with the temperature extremes of the Kalahari whilst minimizing disturbance to the cyanobacteria soil crust. This approach has enabled CO₂ fluxes to be monitored in situ with a high degree of precision for extended periods.

In August 2005, when the surface and subsoils were dry, the ambient CO₂ efflux was negative and low during the daytime (−6.15 mg C m² h⁻¹). When 8 mm rainfall equivalent of water was added to the surface there was an immediate uptake of CO₂ during the daytime at rates up to 75 mg C m² h⁻¹ demonstrating that rates of net photosynthesis are greatly enhanced by available moisture. In contrast, in May 2006 following a prolonged wet period when the subsoil was moist, there was a net positive efflux of CO₂ from the soil at rates of up to 60 mg C m² h⁻¹ irrespective of whether the surface soil was moist or not. This is consistent with subsoil heterotrophic bacterial respiration becoming an important contributor to soil efflux.     

Dynamics of residue decomposition and N2 fixation of grain legumes upon sugarcane residue retention as an alternative to burning

Burning of sugarcane residues contributes to air pollution and sugarcane producers have been forced to abandon it. The change from burning to residue retention is likely to alter the cycling of nutrients. Additionally, there is often a time gap of 6–8 months between two different sugarcane cycles during which legumes could be planted. Thus, the objective of this study was to assess the effects of burning, mulching or incorporation of sugarcane residues on residue decomposition and N mineralization (sugarcane residue management period) and subsequently upon ploughing (legume period) on N dynamics, N₂ fixation, development and nutrient yields of groundnut and soybean grown between two sugarcane cycles on a sandy soil in Northeast Thailand.

Soil microbial biomass N increased when sugarcane residues were incorporated instead of burned or surface applied at 14 days after initiation of cane residue management. Thereafter, high net N mineralization was accompanied by a reduction in microbial biomass N, indicating that mineralized N was derived from microbial N turnover. However, upon ploughing after 96 days the different previous sugarcane residue management strategies had no significant (P > 0.05) effect on net mineral N and microbial biomass N during the subsequent legume period. Although, ¹⁵N enrichment in control reference plants and plant N uptake indicated significant N immobilization effects persisting into the legume crop phase, the proportion of N derived from N₂ fixation (%Ndfa) or amount of N₂ fixed were not significantly different between sugarcane residue management treatments. Soybean fixed more N₂ (78%Ndfa, 234 kg N fixed ha⁻¹) than groundnut (67%Ndfa, 170 kg N fixed ha⁻¹) due to its larger N demand and a poorer utilization of soil N (64 kg N ha⁻¹ vs. 85 kg N ha⁻¹). Groundnut led to a positive soil N balance while that of soybean was negative due to its high nitrogen harvest index. Legume residues returned 61 and 146 kg N ha⁻¹ to the soil for soybean and groundnut, respectively, compared to only 34–39 kg N ha⁻¹ by fallow weeds. Sugarcane residue retention improved soil organic carbon and N content. The results suggested that although a change from burning to sugarcane residues retention led to alterations in N cycling and improved soil organic matter it did not significantly affect N₂ fixation due to the uniforming action of ploughing and the extended time gap between sugarcane residue incorporation and legume planting.     

Potassium retention and leaching in an organic crop rotation on loamy sand as affected by contrasting potassium budgets

Abstract. In organic farming, potassium (K) deficiency may become a significant problem due to nutrient import restrictions. Knowledge about potential K leaching in systems with different K budgets is therefore important for effective agricultural management. We investigated the effect of four organic farming systems (two livestock densities in combination with two types of organic manure) on crop yields, K leaching and K balances in a six course crop rotation from 1993/94 to 1997/98. Average K concentrations in soil water extracted by means of ceramic suction cups at 1 m depth were 0.6 mg K l⁻¹ corresponding to a K leaching loss of 1.5 kg ha⁻¹ yr⁻¹ which was less than expected from values reported in the literature. Variation in K budgets from −12 to +30 kg ha⁻¹ yr⁻¹ did not affect K leaching. In an additional experiment with application of 988 kg K ha⁻¹ as KCl, K leaching accounted for only 0.2% of the applied K although 40% of the accompanying Cl was leached. The main part of the applied K was retained in the topsoil. It was concluded that K leaching was a result of the fertilizer history rather than of the current K budget.     

Aluminum toxicity of synthetic aluminum–humus complexes derived from non-allophanic and allophanic Andosols and its amelioration with allophanic materials

Natural non-allophanic Andosols often show aluminum (Al) toxicity to Al-sensitive plant roots. The significance of Al–humus complexes to Al toxicity has been emphasized. Allophanic Andosols also possess Al–humus complexes, but they rarely show any toxicity. In the present study, using model substances, we tested the toxicity of Al–humus complexes and its amelioration with allophanic materials. We extracted humic substances from the A horizons of a non-allophanic Andosol and an allophanic Andosol using a NaOH solution, and reacted the humic substances and partially neutralized AlCl₃ solution at pH 4. Allophanic material was purified from commercial Kanuma pumice. Plant growth tests were conducted using a medium containing the Al–humus complexes (50 g kg⁻¹), the allophanic material (0, 90, 180 and 360 g kg⁻¹) and perlite. The root growth of barley ( Hordeum vulgare L.) and burdock ( Arctium lappa ) was reduced in the media containing the Al–humus complexes derived from both the non-allophanic and allophanic Andosols when the allophanic material was not added. With the addition of the allophanic materials, particularly in the 360 g kg⁻¹ treatment, the growth of the barley roots was improved markedly. Although the root growth of the burdock tended to improve with allophanic materials, the effect was weaker than that for barley. Monomeric Al in a solution of the medium was not detected (< 0.05 mg L⁻¹) following the addition of 360 g kg⁻¹ of allophanic materials, whereas 0.8–1.7 mg L⁻¹ Al was recorded without the allophanic material.     

Increasing nitrate removal at low temperatures by incorporating organic matter into paddy fields

Denitrification of paddy fields is a key process for improving water quality in fields where nitrate concentrations are high. The objective of the present study was to understand the effects of incorporating organic carbon (C) into soil on the denitrification rate of paddy fields in winter. On 11 December 2007, separate paddy field plots were prepared by incorporating 5 Mg ha⁻¹ of rice straw (RS), 11 Mg ha⁻¹ of rice straw compost (RSC) or a control. A field with a high concentration of nitrate in the water (averaging 18 mg N L⁻¹) was irrigated until 29 March. During the experiment, the daily average soil temperature at a depth of 0.05 m ranged between 3 and 15°C. The nitrate concentration in the surface water in the RS plot, where the residence time was 2 days, decreased more than the concentration in the control or RSC plots. The total estimated nitrate removal from each plot in relation to the other plots was RS > RSC = control. Measurements of the soil from each plot on 29 February 2008 showed that incorporation of RS significantly increased the denitrification potential, even at low temperatures (5–10°C). Furthermore, the RS plot contained more dissolved organic C than the control or RSC plots. This result indicates that supplying RS effectively increases denitrification under low-temperature conditions.