Long-term tillage effects on soil quality

Public interest in soil quality is increasing, but assessment is difficult because soil quality evaluations are often purpose- and site-specific. Our objective was to use a systems engineering methodology to evaluate soil quality with data collected following a long-term tillage study on continuous corn (Zea mays L.). Aggregate characteristics, penetration resistance, bulk density, volumetric water content, earthworm populations, respiration, microbial biomass, ergosterol concentrations, and several soil-test parameters (pH, P, K, Ca, Mg, Total-N, Total-C, NH₄-N, and NO₃-N) were measured on Orthic Luvisol soil samples collected from Rozetta and Palsgrove silt loam (fine-silty, mixed, mesic Typic Hapludalfs) soils. Plots managed using no-till practices for 12 years before samples were collected for this study had surface soil aggregates that were more stable in water and had higher total carbon, microbial activity, ergosterol concentrations, and earthworm populations than either the chisel or plow treatments. Selected parameters were combined in the proposed soil quality index and gave ratings of 0.48, 0.49, or 0.68 for plow, chisel, or no-till treatments, respectively. This indicated that long-term no-till management had improved soil quality. The prediction was supported by using a sprinkler infiltration study to measure the amount of soil loss from plots that had been managed using no-till or mold-board plow tillage. We conclude that no-till practices on these soils can improve soil quality and that the systems engineering methodology may be useful for developing a more comprehensive soil quality index that includes factors such as pesticide and leaching potentials.     

Tillage effects on soil degradation, soil resilience, soil quality, and sustainability

Soil degradation, decrease in soil's actual and potential productivity owing to land misuse, is a major threat to agricultural sustainability and environmental quality. The problem is particularly severe in the tropics and sub-tropics as a result of high demographic pressure, shortage of prime agricultural land, harsh environments, and resource poor farmers who presumably cannot afford science based recommended inputs. Tillage methods and soil surface management affect sustainable use of soil resources through their influence on soil stability, soil resilience, and soil quality. Soil stability refers to the susceptibility of soil to change under natural or anthropogenic perturbations. In comparison, soil resilience refers to soil's ability to restore its life support processes after being stressed. The term soil quality refers to the soil's capacity to perform its three principal functions e.g. economic productivity, environment regulation, and aesthetic and cultural values. There is a need to develop precise objective and quantitative indices of assessing these attributes of the soil. These indices can only be developed from the data obtained from well designed and properly implemented long-term soil management experiments conducted on major soils in principal ecoregions.     

Quantifying soil water effects on nitrogen mineralization from soil organic matter and from fresh crop residues

A loamy sand was incubated with and without addition of carrot leaves at six different water contents ranging from 6% to 20% (g 100 g^-1 dry soil) and N mineralization was monitored during 98 days. We calculated zero- and first-order rates for mineralization in the unamended soil and first-order rates for N mineralization in the residue-amended soil. Although N mineralization was strongly affected by soil moisture, rates were still important at 6% water content (corresponding to permanent wilting point), particularly in the residue-amended soil. Soil water content was recalculated as soil water tension and as percent water-filled pore space (%WFPS) and a parabolic, a logistic and a Gaussian-type function were fitted to the relation between N mineralization rates and water content, %WFPS or pF. Water potential was a less suitable parameter than either %WFPS or water content to describe the soil water influence on N mineralization, because N mineralization rates were extremely sensitive to changes in the water potential in the range of pF values between 1.5 and 2.5. In the residue-amended soil the Gaussian model yielded an optimum %WFPS of 56% for N mineralization, which is slightly lower than optimum values cited in literature. N mineralization in the unamended soil was more influenced by soil water than N mineralization from fresh crop residues. This could be explained by less water limitation of the microbial population decomposing the residues, due to the water content of the residues. The effect of the water contained in the residues was most pronounced in the lowest water content treatments. The water retention curves of both undisturbed and repacked soil were determined and suggested that extrapolation of results obtained during laboratory incubations, using disturbed soil, to field conditions will be difficult unless soil bulk density effects are accounted for, as is the case with the use of %WFPS.     

Carbon mineralisation in soil adjacent to plant residues of contrasting biochemical quality

A fraction of the C of residues incorporated into soil diffuses into the adjacent soil where it is eventually mineralised by microorganisms. Our aim was to quantify the contribution of this adjacent soil to the overall mineralisation of residue-C. For this, we incorporated two different residues labelled with ¹³C, with contrasting biochemical characteristics, namely mature wheat straw and young rye leaves, in soil cores. When 15% mineralisation of residue-C was measured for both residues, we separated a particulate fraction (the residues), the adjacent soil (4-5 mm thick) and a distant soil fraction, and incubated them separately for 5 h. We found that 76% of the mineralised wheat straw-C came from the particulate fraction and 23% from the soil adjacent to the residues. For rye leaves, 67% of the evolved CO₂ came from the particulate fraction and 33% from the adjacent soil. It showed that the adjacent soil had a significant role in the mineralisation of carbon from the residues, even if the main source of residue-derived CO₂ was the particulate fraction itself. The functional importance of the soil adjacent to the residues increased with the amount of soluble organic compounds that had been leached from the residue into the adjacent soil, suggesting a strong interaction between the initial quality of the crop residue and the resulting spatial heterogeneity of the decomposing microorganisms and C within the soil.     

Land management effects on the near-surface physical quality of a clay loam soil

Although agricultural land management is known to affect near-surface soil physical quality (SPQ), the characteristics of these affects are poorly understood, and diagnostic SPQ indicators are not well-developed. The objective of this study was to measure a suite of potential SPQ indicators using intact soil cores and grab samples collected from the 0–10 cm depth of a clay loam soil with the treatments: (i) virgin soil (VS); (ii) long-term continuous bluegrass sod (BG); (iii) long-term maize (Zea mays L.)—soybean (Glycine max (L.) Merr.) rotation under no-tillage (NT); (iv) long-term maize–soybean rotation under mouldboard plough tillage (MP); (v) short-term (1–4 years) NT after long-term MP; (vi) short-term MP after long-term BG; (vii) short-term MP after long-term NT. Organic carbon content, dry bulk density, air capacity, relative water capacity and saturated hydraulic conductivity appeared to be useful SPQ indicators because they were sensitive to land management, and proposed optimum or critical values are available in the literature. Soil macroporosity was also sensitive to land management, but optimum or critical values for this parameter are not yet established. Soil matrix porosity and plant-available water capacity did not respond substantially or consistently to changes in land management, and were thus not useful as SPQ indicators in this study. Converting long-term BG to MP caused overall SPQ to decline to levels similar to long-term MP within 3–4 years. Converting long-term NT to MP or vice versa caused only minor changes in overall SPQ. With respect to the measured SPQ indicators and their optimum or critical values, both VS and BG produced “good” overall SPQ in the near-surface soil, while long-term maize–soybean rotation under NT and MP produced equally “poor” SPQ.     

Long-term soil management effects on crop yields and soil quality in a dryland Alfisol

A long-term experiment was conducted with the objective of selecting the appropriate land management treatments and to identify the key indicators of soil quality for dryland semi-arid tropical Alfisols. The experiment was conducted using a strip split–split plot design on an Alfisol (Typic Haplustalf) in southern India under sorghum (Sorghum vulgare (L))-castor (Ricinus communis (L)) bean rotation. The strip constituted two tillage treatments: conventional tillage (CT) and minimum tillage (MT); main plots were three residues treatments: sorghum stover (SS), gliricidia loppings (GL), ‘no’ residue (NR) and sub plots were four nitrogen levels: 0 (N₀), 30 (N₃₀), 60 (N₆₀), and 90 kg ha⁻¹ (N₉₀). Soil samples were collected after the sixth and seventh year of experimentation and were analyzed for physical, chemical and biological parameters. Sustainable yield index (SYI) based on long-term yield data and soil quality index (SQI) using principal component analysis (PCA) and linear scoring functions were calculated. Application of gliricidia loppings proved superior to sorghum stover and no residue treatments in maintaining higher SQI values. Further, increasing N levels also helped in maintaining higher SQI. Among the 24 treatments, the SQI ranged from 0.90 to 1.27. The highest SQI was obtained in CTGLN₉₀ (1.27) followed by CTGLN₆₀ (1.19) and MTSSN₉₀ (1.18), while the lowest was under MTNRN₃₀ (0.90) followed by MTNRN₀ (0.94), indicating relatively less aggradative effects. The application of 90 kg N ha⁻¹ under minimum tillage even without applying any residue (MTNRN₉₀) proved quite effective in maintaining soil quality index as high as 1.10. The key indicators, which contributed considerably towards SQI, were available N, K, S, microbial biomass carbon (MBC) and hydraulic conductivity (HC). On average, the order of relative contribution of these indicators towards SQI was: available N (32%), MBC (31%), available K (17%), HC (16%), and S (4%). Among the various treatments, CTGLN₉₀ not only had the highest SQI, but also the most promising from the viewpoint of sustainability, maintaining higher average yield levels under sorghum–castor rotation. From the view point of SYI, CT approach remained superior to MT. To maintain the yield as well as soil quality in Alfisols, primary tillage along with organic residue and nitrogen application are needed.     

Crop production impacts on soil phosphorus removal and soil test levels

An 8-year field study documented the impact of tillage, crop rotations, and crop residue management on agronomic and soil parameters at Brookings, South Dakota. The greatest annual proportion of above-ground biomass phosphorus (P) removed was from the grain (78–87% of total) although crop residue removed some P as well. Greater above-ground total biomass P (grain P + crop residue P) was removed from corn than from soybean and spring wheat crops mainly due to the greater corn grain biomass harvested. Cumulative above-ground biomass P removal was greatest for the corn-soybean rotation (214 kg P ha^?1), while it was lowest for the soybean-wheat rotation (157 kg P ha^?1). Tillage treatments within crop rotation or residue management treatments did not influence annual or cumulative P removal rates. Olsen extractable soil orthophosphate-P levels declined consistently through time from a mean of 40 µg g^?1 (2004) to 26 µg g^?1 (2011). Biomass P removal was calculated to be 15.7 ha^?1 yr^?1 to decrease Olsen extractable soil orthophosphate-P levels by 1 µg g^?1 yr^?1 over 8 years of the study.     

An initial study on soil wettability effects during entrapped LNAPL removal by surfactant flooding in coarse-grained sand media

Purpose

Non-ideal or fractional wettability conditions may exist at field sites. It was hypothesized that fractional wettability could cause larger entrapped LNAPL saturations and unexpected soil interactions during surfactant remediation. Soil wettability effects during entrapped LNAPL recovery by surfactant flooding in coarse-grained sand were investigated through a comparative study. The main objective was to identify the impacts of soil wettability on LNAPL removal via submicellar concentration surfactant flooding to remobilize entrapped LNAPL.

Materials and methods

A baseline for comparison was established by testing an ideal water-wet soil (silica sand) along with a fractionally wet soil. Two LNAPLs were tested: LNAPL from a contaminated field site and heptane, which represented an ideal lab-grade fluid. Contact angle, interfacial tension, capillary pressure–saturation, and column tests were performed to characterize wettability and identify the effects of soil wettability during entrapped LNAPL recovery. Two anionic surfactants were used for the column experiments: sodium dodecyl-benzene-sulfonate and a field site anionic surfactant. To further investigate the effects of LNAPL contact time within the soil, columns tests were performed at two different LNAPL contact times.

Results and discussion

Contact angle measurements and column tests conducted with the field LNAPL revealed its potential to establish non water-wet wettability conditions. Column test results indicated that fluid entrapment was independent of fluid type, and the wettability and contact time conditions studied. Entrapped LNAPL saturations after water flooding were approximately 19 %. Entrapped LNAPL removal by mobilization occurred at the predetermined submicellar surfactant concentration (0.5 g/L) with a maximum removal of 43 %. Entrapped LNAPL removal from fractionally wet columns was higher in comparison to water-wet columns and was found to increase with contact time.

Conclusions

Entrapped LNAPL saturations after water imbibition were not impacted by the wettability conditions studied. The fractionally wet soil behaved differently during the surfactant-flood; higher LNAPL removal was achieved suggesting that non-ideal wettability had a positive impact. The presence of NAPL-wet mineral grains might have favored pore scale interactions causing NAPL redistribution and increasing NAPL-surfactant solution interfacial areas contributing to LNAPL removal. Pore scale studies and subsequent testing is recommended to further this study’s findings.     

The effects of planting systems on soil biology and quality attributes of tomatoes

This study was carried out to determine the effects of seedling types (grafted and non-grafted) and different plantation systems (raised-bed and flat planting) on growth, yield and quality of tomato (Solanum lycopersicum cv. Depar F1) plants organically grown in open-field conditions in Samsun. Soil microbial biomass-C increased by 25% and soil CO₂ contents increased by 16% in raised-bed systems. It was observed that soil microbial biomass-C positively correlated with CO₂ contents, leaf chlorophyll content, stomatal conductance and yield (P < 0.05 and P < 0.01) and negatively correlated with soil compaction. The highest chlorophyll content (47.37 CCI), fruit shape index (1.21) and yield (1.95 kg plant^?1) were obtained from grafted seedlings of the raised-bed planting systems. The highest stomatal conductance (118.50 mmol m^?2 s^?1) and firmness (79.34%) were obtained from non-grafted seedlings of the raised-bed planting systems. As a result, successful tomato cultivation was carried out with the use of raised-bed and grafted seedlings. However, non-grafted seedlings of the raised-beds had higher yield and quality values than the grafted seedlings of the flat planting.     

Mineralisation of crop residues on the soil surface or incorporated in the soil under controlled conditions

In the present work, we compare the effect of mature crop residues mixed into a ferralitic soil or placed as a single layer on soil surface on the mineralisation of C and N over 55 days. As residues, we used dry stems of rice, soybean, sorghum, brachiaria and wheat. There were no significant effects of residue placement on C mineralisation kinetics. Decomposition of the residues on the soil surface slightly increased net N mineralisation for residues having the smallest C/N ratio.     

Assessment of soil flooding in cities by electrophysical methods

The degree and localization of soil flooding have been assessed in the cities of Rostov Velikii and Astrakhan by the vertical electric sounding method. It has been proved that this method permits one to reliably reveal strongly excessively moistened (flooded) soils and to discover the groundwater level using measurements from the surface without digging profiles and boring drill holes.     

Medium-term effects of a single application of mustard residues on soil microbiota and C content of vertisols

 A study of the effects of different qualities (fresh and composted) and rates (equivalent to 120, 240, and 360 kg N ha^–1) of mustard meal application on wheat yields on humid tropical vertisol was started in 1990 at Ginchi Research Station in Ethiopia. After continuous wheat cropping for 7 years and without any further fertilisation, soil microbial parameters (basal respiration, microbial biomass-C and N, organic-C, and ecophysiological quotients) were studied during one growth period. After 7 years of application, mustard meal still exerted a significant positive effect on microbial biomass, basal respiration, organic-C, C_mic : N_mic ratio, and metabolic quotient (qCO₂). Organic-C, qCO₂ and C_mic : N_mic ratios were higher for the compost-amended plots than plots amended with fresh mustard meal. Basal respiration, C_mic, and C_mic : N_mic ratio showed a clear seasonality, but only in manured plots. The data indicate shifts in microbial community structure (from bacteria to fungi and from r to K strategists) and suggest positive medium-term effects of mustard meal on humid tropical vertisol biological qualities. Received: 25 May 1999     

The quality of exogenous organic matter: short-term effects on soil physical properties and soil organic matter fractions

We examined the short-term effect of five organic amendments and compared them to plots fertilized with inorganic fertilizer and unfertilized plots on aggregate stability and hydraulic conductivity, and on the OC and ON distribution in physically separated SOM fractions. After less than 1 year, the addition of organic amendments significantly increased ( P  <   0.01) the aggregate stability and hydraulic conductivity. The stability index ranged between 0.97 and 1.76 and the hydraulic conductivity between 1.23 and 2.80 × 10⁻³ m/s for the plots receiving organic amendments, compared with 0.34–0.43, and 0.42–0.64 × 10⁻³ m/s, respectively, for the unamended plots. There were significant differences between the organic amendments (P <  0.01), although these results were not unequivocal for both soil physical parameters. The total OC and ON content were significantly increased ( P  <   0.05) by only two applications of organic fertilizers: between 1.10 and 1.51% OC for the amended plots versus 0.98–1.08% for the unamended and between 0.092 and 0.131% ON versus 0.092–0.098% respectively. The amount of OC and ON in the free particulate organic matter fraction was also significantly increased ( P  <   0.05), but there were no significant differences ( P  <   0.05) in the OC and ON content in the POM occluded in micro-aggregates and in the silt + clay-sized organic matter fraction. The results showed that even in less than 1 year pronounced effects on soil physical properties and on the distribution of OC and ON in the SOM fractions occurred.     

Effect of soil copper on the response of soil fungal communities to the addition of plant residues

Organic residues provide the fundamental energy supply supporting soil fungal communities. Provision of adequate energy is required for soil microbial communities to adapt and function in the presence of ecological stress, such as copper (Cu) contamination. However, contamination can also lead to decreased ecological fitness of microorganisms, limiting their ability to access substrates. Thus, complex interactions exist between substrates, metals, energy supply/accessibility, fungal communities and their processes, and these have implications for ecosystem processes. We investigated the interaction between energy resources and Cu tolerance on soil fungal communities, including Fusarium and Trichoderma (model disease causing and beneficial genera). Using quantitative PCR and DGGE fingerprinting, the effects of increasing soil Cu levels (0 to >3000 mg Cu kg⁻¹ soil) on size and structure of soil fungal communities were tested under basal and plant-residue (medic; Medicago trunculata) added conditions. The interaction between increasing soil Cu levels and the addition of plant resources on fungal community structure was tested using multivariate analysis. The relative size (DNA copies per unit of soil DNA) of soil fungal communities, including Trichoderma and Fusarium, significantly (P < 0.05) increased (94% and 32% respectively) with addition of medic to soil. In medic-applied samples, the bacterial to fungal ratio decreased, demonstrating the selective influence of the cellulose-rich substrate on the fungal community. Under the high nutrient conditions fungal DNA increased as a fraction of the total soil DNA, demonstrating the tolerance of fungi to Cu (relative to other microbiota) given adequate energy resources. Copper had no impact on the abundance of Fusarium or Trichoderma, but significantly affected community structure (PERMANOVA; P < 0.05). With increasing Cu, species selection and replacement could be observed, particularly in soils where medic had been included. Plant residue addition itself was a highly selective factor affecting the structure of communities of Trichoderma and Fusarium (P < 0.05). The effects of increasing Cu could be seen in both medic and basal soils for Trichoderma, but only in the basal treatments for soil Fusarium. This was due to very low dispersion in Fusarium community structure in the medic-added treatment (PERMDISP; P < 0.05). The results show the interactive influence of organic matter inputs and heavy metal contamination on size and structure of soil fungal communities. The data show that species selection and replacement is an important mechanism for community adaptation to increasing levels of soil Cu, and this mechanism can be influenced by addition of resources to the soil.     

Critical body residues (CBRs) for ecotoxicological soil quality assessment: copper in earthworms

The term ‘critical body residue’ (CBR) was defined as the lowest observed total body concentration of a contaminant in an organism, which is associated with the occurrence of adverse toxic effects in either individuals or populations of a defined age or stage of development. In this study, internal toxicity thresholds were determined for copper in the clitellated adult stage of earthworms (Lumbricus rubellus and Aporrectodea caliginosa). The objective was to assess the applicability of CBRs as a practical tool in soil quality assessment of contaminated sites and as a means of a sustainable protection of earthworm fauna. Laboratory studies showed that body concentrations of Cu were generally in agreement with the chemically available CaCl₂-extractable fraction in soil, but that there was also some evidence of internal pH-related homeostatic regulation. Toxicological correlates of body Cu concentrations with adverse effects on cocoon production (fecundity) suggested an approximate sublethal internal threshold of about 40 mg kg⁻¹, with mortality occurring at about 60 mg kg⁻¹. Adult L. rubellus sampled from areas with a wide range of metal pollution showed body Cu concentrations with a minimum of 8 mg kg⁻¹ and a maximum of 60 mg kg⁻¹. Beyond this apparent physiological tolerance range, environmental management directed at optimal earthworm population survival may not be sustainable in contaminated fields. Studies of L. rubellus colonizing a metal-contaminated experimental sludge-treated field showed that a reduced rate of colonization can already be associated with an average body Cu concentration of 25 mg kg⁻¹. However, in this particular field situation mixture effects of other metals that were also present in the soil and the occurrence of avoidance behaviour during colonization may have contributed to this low internal toxicity threshold. It is concluded that the CBR approach seems to be a feasible option for use as a tool in a bioavailability-based soil quality assessment, even for essential trace metals like copper, but that further insight may be needed to establish the uncertainty and reliability of the application in environmental quality assessment and decision making.     

Contrasting grain crop and grassland management effects on soil quality properties for a north-central Missouri claypan soil landscape

Abstract

Crop management has the potential to either enhance or degrade soil quality, which in turn impacts on crop production and the environment. Few studies have investigated how crop management affects soil quality over different landscape positions. The objective of the present study was to investigate how 12 years of annual cropping system (ACS) and conservation reserve program (CRP) practices impacted soil quality indicators at summit, backslope and footslope landscape positions of a claypan soil in north-central Missouri. Claypan soils are particularly poorly drained because of a restrictive high-clay subsoil layer and are vulnerable to high water erosion. Three replicates of four management systems were established in 1991 in a randomized complete block design, with landscape position as a split-block treatment. The management systems were investigated: (1) annual cropping system 1 (ACS1) was a mulch tillage (typically ≥ 30% of soil covered with residue after tillage operations) corn (Zea mays L.)–soybean (Glycine max (L.) Merr.) rotation system, (2) annual cropping system 2 (ACS2) was a no-till corn–soybean rotation system, (3) annual cropping system 3 (ACS3) was a no-till corn–soybean–wheat (Triticum aestivum L.) rotation system, with a cover crop following wheat, (4) CRP was a continuous cool-season grass and legume system. In 2002, soil cores (at depths of 0–7.5, 7.5–15 and 15–30 cm) were collected by landscape position and analyzed for physical, chemical and biological soil quality properties. No interactions were observed between landscape and crop management. Relative to management effects, soil organic carbon (SOC) significantly increased with 12 years of CRP management, but not with the other management systems. At the 0–7.5-cm soil depth in the CRP system, SOC increased over this period by 33% and soil total nitrogen storage increased by 34%. Soil aggregate stability was approximately 40% higher in the no-till management systems (ACS2 and ACS3) than in the tilled system (ACS1). Soil aggregation under CRP management was more than double that of the three grain-cropping systems. Soil bulk density at the shallow sampling depth was greater in ACS3 than in ACS1 and ACS2. In contrast to studies on other soil types, these results indicate only minor changes to claypan soil quality after 12 years of no-till management. The landscape had minor effects on the soil properties. Of note, SOC was significantly lower in the 7.5–15-cm soil depth at the footslope compared with the other landscape positions. We attribute this to wetter and more humid conditions at this position and extended periods of high microbial activity and SOC mineralization. We conclude that claypan soils degraded by historical cropping practices will benefit most from the adoption of CRP or CRP-like management.     

Modeling consequences of straw residues export on soil organic carbon

Cereal straw, which is most often returned to the soil in arable cropping systems, is of renewed interest as a potential source of bioenergy. However, the sustainability of this practice which implies systematic removal of aerial biomass of cereal crops is a controversial issue, particularly in soils having a low soil organic carbon (SOC) content. This study aims at evaluating a simple model (AMG) to predict the consequences of straw export on SOC evolution in various cropping and pedoclimatic conditions. The model was tested on nine long-term field experiments (18–35 yr) dominated by cereal crops and differing in climate, soil type and carbon inputs. The model was able to provide satisfactory simulations of the evolution of SOC in most experiments with a unique set of parameters. The sensitivity analysis indicated that the quality of fit was very sensitive to humification coefficient, moderately sensitive to the size of the stable SOC pool and weakly affected by the ratio of belowground: aerial C input. The dependence of model parameters (humification and mineralization rates) on pedoclimatic conditions (soil clay content and temperature) was analyzed and compared to those proposed in other models (DAISY, CENTURY, ROTHC, CN-SIM) since they vary widely between models. AMG functions provided the best fit in seven out of nine experiments. More generally, the best fit was obtained by assuming that clay content had a small or no effect on humification coefficient and a marked effect on mineralization rate, in accordance with incubation studies in literature. The AMG model was used to simulate the impact of a straw export scenario in nine experiments considering a systematic straw removal one year out of two. With this scenario, straw removal vs. incorporation would reduce carbon stocks by 2.5–10.9% of the initial SOC after 50 yr, depending primarily on the experiment (soil, climate, productivity) and secondarily on the size of the stable C pool (varying from 10% to 65%).     

Green manure effects on soil quality in relation to suppression of Verticillium wilt of potatoes

Verticillium dahliae is a major, persistent pathogen in soil but conventional control is becoming more difficult because of increasing costs and environmental impacts of pesticides. Organic amendments can naturally suppress plant diseases, but to be reliable, mechanisms and suppressive soil indicators need to be understood. Consequently, a 3 × 3 factorial experiment was conducted in two separate fields over 2 years with three green manure types (Austrian winter pea, Pisum sativum L.; broccoli Brassica oleracea L.; or Sudan grass, Sorghum vulgare), incorporated at three rates (6, 12, or 24 Mg ha⁻¹ dry biomass). The relationship between soil chemical and microbiological properties and suppression of Verticillium wilt of potato was investigated using correlation and stepwise multiple-linear-regression (MLR) analysis. V. dahliae inoculum density (ID) were positively correlated with relative area under the senescence progress curve (RAUSPC) in both 2002 and 2003. In 2002, in addition to ID, low soil pH, low Ca, high K, high Mg, high total soil C, and low arylsulfatase activity were associated with low RAUSPC. Soil pH, Ca, K, and Mg were not impacted by green manure treatments, but rather indicated a pre-existing soil gradient at the 2002 site. In 2003, in addition to ID, high values of NO₃ ⁻−N, total C, fluorescein diacetate hydrolysis (FDA), microbial respiration, and microbial biomass C were associated with low RAUSPC. These six factors were affected by green manure treatments. The best MLR model included terms for ID, FDA, and soil pH, and accounted for 70% of the variability in RAUSPC.     

Influence of temporary flooding at three growth stages on soybeans grown on a clayey soil

Abstract

Knowledge of crop response to temporary waterlogging is important in the development of effective water management practices. A field study was conducted to determine the response of soybean [Glvcine max. (L.) Merr] grown on a poorly drained, clayey soil to temporary flooding at three growth stages. The four treatments were soybean flooded for seven consecutive days at either VI, V4 or R2 growth stages at a flood height of 0.03 m above the soil surface and a well‐watered control. Flooding for seven consecutive days wetted but did not saturate the lower parts of the soil profile. This was attributed to the swelling by the montmorillonitic clay and subsequent sealing of the soil near the surface. In general, Eh and ODR decreased gradually during the flood, but increased as the soil dried upon removal of the flood. Canopy heights and dry weights of the flooded soybeans were dependent on plant growth stage at flooding and time of measurement but were lower than the control. When flooded at the VI or V4 growth stages, concentrations of N and K in the above‐ground plants were lower than the control after the flood. Three weeks after the flood was removed higher concentrations of these elements were found. When flooded at R2, concentrations of N and K were lower than in the control. Few differences were found in the plant concentrations of Ca, Mg, Mn, Fe, Al, and Na in the plant due to the flood, but by the end of the growing season, concentrations of Mn, Fe and Al were higher in the R2 flooded soybeans than in the other treatments. Seed yield response of the soybeans depended upon plant growth stage at flooding. The soybeans were particularly sensitive to the seven days of continuous flood at the R2 growth stage. Values of relative seed yield were 88, 83, and 44 % of the well watered‐control for the VI, V4 and R2 growth stages, respectively. Differences in seed yield were found with cultivar and with cultivar?flood treatment.