Continuous and pulse feedings of urea as a nitrogen source in fed-batch cultivation of Spirulina platensis

Spirulina platensis was autotrophically cultivated by fed-batch addition of urea as a nitrogen source. Continuous and pulse feeding regimes of this nitrogen source (time intervals of 24 h) were compared using a constant mass flow rate. Both series of experiments were carried out at three different temperatures (25, 28 and 31 °C) and total times of urea addition (12, 15 and 18 days), according to a 2² simple factorial design, and the effects of these variables on the nitrogen-to-cell conversion factor and cell productivity were verified by analysis of covariance (ANCOVA). The intermittent addition of urea yielded results similar to those obtained by the continuous feeding, therefore, the former operation mode would be preferable to reduce the production costs of this cyanobacterium in large-scale facilities.     

Substitution of Chaetoceros muelleri by Spirulina platensis meal in diets for Litopenaeus schmitti larvae

The nutritional response of Litopenaeus schmitti larvae to substitution of Chaetoceros muelleri by Spirulina platensis meal (SPM) was evaluated. The substitution levels (S) were 0%, 25%, 50%, 75% and 100%, dry weight basis. Final larval length (FL) ranged from 1.98 to 3.16 mm for the different substitution levels. There was a significant relationship between S and FL, described by the following quadratic equation: FL = 2.853 + 0.01598S − 0.000233S². The substitution level (S) yielding maximum FL was 34.2%. Development index (DI) values ranged from 2.84 to 3.93 and were dependent on substitution level. The corresponding equation was DI = 3.799 + 0.00945S − 0.000189S² (P < 0.01). Maximum DI was obtained at 25.0% substitution. Survival was high (82–87%) and no significant differences were found between treatments. Protein digestibility of either microalgae was high, with 92% for SPM and 94% for C. muelleri, with no significant differences between them. The results in this study indicate that an adequate balance of nutrients in relation to the requirements of the species is critical. To simultaneously improve FL and DI, a 30% substitution of C. muelleri by SPM is suggested. This is equivalent to feeding 0.15 mg larvae^− 1 day^− 1 dry weight basis of a 70% C. muelleri/30% SPM diet, representing 0.078 mg protein larvae^− 1 day^− 1, 0.026 mg lipids larvae^− 1 day^− 1 and 2.732 J larvae^− 1 day^− 1.     

The effects of the decomposition of mangrove leaf litter on water quality, growth and survival of black tiger shrimp (Penaeus monodon Fabricius, 1798)

An experiment was conducted for 8 weeks at the Cantho University, Vietnam, to determine the acceptable level of mangrove leaf litter load and its effect on water quality, growth and survival rate of tiger shrimp (Penaeus monodon). Shrimps were cultured in plastic tanks containing 50 L of brackish water (salinity of 15‰). Leaf litter of Rhizophora apiculata, Avicennia officinalis, Excoecaria agallocha and Acacia auriculiformis were loaded to tanks at rates of 0.0 (control), 0.125, 0.25, 0.5, 1.0 and 2.0 g L^− 1 with and without aeration. Tiger shrimp post-larvae (PL; 0.05 ± 0.01 g) obtained from the shrimp hatchery of Cantho University were stocked at a density of 20 PL per tank and fed with pelleted feed containing 38% protein at a rate of 10% body weight (BW) day^− 1.

The high leaf-loading rates significantly reduced dissolved oxygen (DO) and survival rates of shrimp in the non-aerated treatments, and all shrimps died after 2 days in the treatments with loading rates above 0.5 g L^− 1. Leaf litter loads significantly increased tannin content, chemical oxygen demand (COD), H₂S and pH in the aerated treatments. Stepwise regression analysis showed COD, tannin and H₂S concentrations had negative effects on shrimp growth in the aerated treatments. Tannin concentration was found to be highest in the treatments with Excoecaria (32 mg L^− 1) and Avicennia (24 mg L^− 1) leaves. However, there were no significant differences in growth and survival rates of shrimp among the aerobic treatments loaded with different leaf types. The results of this study showed that moderate load of mangrove leaves could play an important role in promoting shrimp growth and survival in aerobic condition. Mangrove leaves at a loading rate of 1 g L^− 1 positively influenced both the survival and growth rate of shrimps.     

小球藻与芽孢杆菌对对虾养殖水质调控作用的研究

通过在凡纳滨对虾养殖水体中添加小球藻和芽孢杆菌,研究其对养殖水质的调控作用。结果表明,小球藻和芽孢杆菌联合处理组对水质的调控效果优于只添加芽孢杆菌组或小球藻组。菌-藻联合处理组能很好地降低水体中氮、磷的含量,对氨氮的作用尤为明显;实验进行的第5天内,NH4 -N含量显著低于对照组(P<0·05),降低率为32·94%,NO2--N降低率为10·29%,PO34--P降低率为36·02%。小球藻 芽孢杆菌组NH4 -N含量平均为0·277mg/L,日均积累速率0·0135mg/L·d,而对照组为0·0472mg/L·d;NO2--N平均含量为0·334mg/L,日均积累速率为0·0617mg/L·d。小球藻在调控水质的同时也向水体释放有机物,从而引起水体COD的上升。     

两种典型对虾养殖模式的经济与生态效益分析

为探讨贝、藻偶合方式对集约化养殖尾水的净化作用,研究分析了香港牡蛎 (Crassostrea hongkongensis) 和钝顶螺旋藻 (Spirulina platensis) 耦合对富含氮 (N)、磷 (P) 营养盐的生物絮团养殖尾水的处理效果。实验共设置5组,小规格组 (S)、中规格组 (M) 和大规格组 (L) 添加的牡蛎个体均质量分别为 (50.99±7.01)、(100.25±8.87) 和 (148.81±15.61) g,阴性对照组 (NC) 只添加螺旋藻,空白对照组 (BC) 不添加牡蛎和螺旋藻,实验组中螺旋藻密度约为 8×10⁵ 个·mL⁻¹,牡蛎生物量为3 kg·m⁻³。记录牡蛎的成活率及生长情况,显微镜计数螺旋藻密度,测定水体氨氮 (相似文献   

Water velocity in commercial RAS culture tanks for Atlantic salmon smolt production

An optimal flow domain in culture tanks is vital for fish growth and welfare. This paper presents empirical data on rotational velocity and water quality in circular and octagonal tanks at two large commercial smolt production sites, with an approximate production rate of 1000 and 1300 ton smolt/yr, respectively. When fish were present, fish density in the two circular tanks under study at Site 1 were 35 and 48 kg/m³, and that in four octagonal tanks at Site 2 were 54, 74, 58 and 64 kg/m³, respectively. The objective of the study was twofold. First, the effect of biomass on the velocity distribution was examined, which was accomplished by repeating the measurements in empty tanks under same flow conditions. Second, the effect of operating conditions on the water quality was studied by collecting and analysing the water samples at the tank’s inlet and outlet. All tanks exhibited a relatively uniform water velocity field in the vertical water column at each radial location sampled. When fish were present, maximum (40 cm/s) and minimum (25–26 cm/s) water rotational velocities were quite similar in all tanks sampled, and close to optimum swimming speeds, recommended for Atlantic salmon-smolt, i.e., 1–1.5 body lengths per second. The fish were found to decrease water velocity by 25% compared to the tank operated without fish. Flow pattern was largely affected by the presence of fish, compared to the empty tanks. Inference reveals that the fish swimming in the tanks is a major source of turbulence, and nonlinearity. Facility operators and culture tank designers were able to optimize flow inlet conditions to achieve appropriate tank rotational velocities despite a wide range of culture tank sizes, HRT’s, and outlet structure locations. In addition, the dissolved oxygen profile was also collected along the diametrical plane through the octagonal tank’s centre, which exhibits a close correlation between the velocity and oxygen measurements. All tanks were operated under rather intensive conditions with an oxygen demand across the tank (inlet minus outlet) of 7.4–10.4 mg/L. Estimates of the oxygen respiration rate in the tank appears to double as the TSS concentration measured in the tank increases from 3.0 mg/L (0.3 kg O₂/kg feed) up to 10–12 mg/L (0.7 kg O₂/kg feed). Improving suspended solids control in such systems may thus dramatically reduce the oxygen consumption and CO₂ production.     

Determining the bottom soil sampling rate in shrimp ponds using variograms

In aquaculture, methods for collecting soil, that represent the mean concentrations of its constituents are not well established. We use the variogram technique to determine the distance between two locations where soil phosphorous is no longer correlated. Using this technique, we estimate that samples need to be taken between 15 and 20 m apart to compose a composite sample that represents the average amount of phosphorus in the soil of four Texas shrimp ponds. Broad spatial trends in phosphorus levels are observed and the trend is qualitatively common to all four ponds. The similarities among three of the four ponds suggest that the results are applicable to similarly managed shrimp ponds in Texas, and elsewhere throughout the world.     

EM在水泥池封闭、半封闭式生态养虾中的应用

本实验以EM作为饲料添加剂和水质净化剂,采用全封闭、半封闭与传统养殖方式—对照组在实验室水泥池(118d)养殖实验。结果表明:EM能改良水质条件,促进对虾生长。全封闭和半封闭实验组成活率分别比对照组提高了48 7%,24 1%;饵料系数则降低了21 2%,14 1%;全封闭和半封闭实验组DO含量与对照组相比分别提高了9 4%,12 6%;COD含量与对照组相比分别降低了0 25%,0 48%;NH 2-N含量与对照4-N含量与对照组相比分别降低了19 2%,19 2%;NO 组相比分别降低了15 2%,21 1%。     

钝顶螺旋藻在养虾废水中的生长研究

研究了钝顶螺旋藻(Spirulina platensis)在养虾废水中的生长情况。结果表明,钝顶螺旋藻不能直接在该养殖废水中生长,但在废水中添加5%比例的Zarrouk培养液后,钝顶螺旋藻能在其中较好地生长。通过正交试验获得了钝顶螺旋藻在含5%Zarrouk培养液的废水中的生长最佳条件:pH=8,初始密度A560为0.3,光强2500lx。在该条件下,钝顶螺旋藻对废水中的氮、磷均有较好的清除效果,氨氮、硝态氮、亚硝态氮和活性磷的清除率分别达到64.5%、41.9%、67.1%和43.9%。     

Improving feeding strategies for shrimp farming using fuzzy logic,based on water quality parameters

In intensive shrimp farming systems, formulated feed represents the main nutrition source and its adequate management significantly influences the economic feasibility of the farm. Based on that, the present study evaluated two dynamic feeding strategies: fuzzy logic (FL) and mathematical functions (MF). For both strategies, the temperature and dissolved oxygen were modified in a controlled way. A conventional feeding table was the control treatment. The results showed that DO was the parameter that mostly influences the feeding rate (74%) while the temperature also did it, but in a lower grade (26%). The results showed that feed conversion rate (FCR) was significantly better when the FL strategy was used, saving around 35% of feed when compared to the control. An expert system based on FL may replace the traditional feeding strategies with no significant adverse effects on growth, survival and FCR, and may easily be adapted to some other culture systems.     

河口区斑节对虾淡化养殖塘水化学状况与水质管理模式

为斑节对虾（Penaeus monodon)淡化养殖设计了3种水质管理模式（各设2个水塘）：（1）全封闭式，将纳水河水注入池后不再换水，仅靠降雨淡化盐度；（2）半封闭式，初始水均取自经贮水塘沉淀、曝晒数日的河口水，水深为池深的80%，待幼虾全长至4cm时，据水质状况添加河水；（3）逐步加淡水递降盐度方式，幼虾长至4cm时，每隔1-2d添加2-5cm河水，水满时排出部分水，日均降盐度百分比为0.2%-1.6%，幼虾长至8-10cm时，池水最终盐度可降至淡水范围（S≥0.55)。结果显示，3种方法养殖均获成功，单产为2340-2557kg/hm^2；方法（1）塘的成活率最高，为66.4%-67.3%，盐度降率为12.3%-16.4%，主要离子总量降率为10.9%-19.0%；方法（2）因只添加少量河水，盐度降率仅为32.2%-48.3%，主要离子总量降率为39%-64%；方法（3）的水质类型始终为海水型（ClⅢ^Na），盐度降率52.8%-93.2%，主要离子总量降率为50%-96%。各淡化塘全周期有效氮总量较低，平均为0.21-0.50mg/L，其中NH3-Nt含量占66%以上，DO均呈过饱和状态（109%-198%），pH与COD较高，分别为8.79-9.25与16.55-24.21mg/L。     

Characterization of water quality in shrimp ponds of different sizes and with different management regimes using multivariate statistical analysis

Coastal shrimp aquaculture in Bangladesh is mostly practiced in an agricultural unit called a Gher, which is a special type of agricultural field with elevated surrounding embankments/borders situated by the side of a river that is used to grow rice in winter and shrimp in summer. Ghers of different sizes are managed in different ways. An on-farm project was carried out to study the effects of Gher size and their related management practices on water quality, shrimp production and the economic returns of shrimp farming in the Southwest coastal region of Bangladesh. We present here the results of this project with respect to water quality as explored through factor analysis, which was applied as a way to understand the nature and extent of the effects of different variables. For the majority of the variables and factors, one-half to two-thirds of their variability was explained by changes over time (months), and a further one-quarter to one-third was explained by Gher size and associated management practices. The alternating dry/monsoon season and water management practices determined long-term and large-scale (annual) water exchange in the Ghers that mainly affected processes related to live and dead particles suspended in the water column. Processes related to decomposition and nitrification were more dependent on short-term (days) water circulation in the Ghers, which is turn was reliant on the time span between the exchanges of water in the Ghers and river. Our results show that improved management leads to higher natural and shrimp productivity in the smaller Ghers and strongly support the argument put forward by the policy makers and scientific community in Bangladesh that the smaller the Gher, the better they can be managed and the better the possibility of increased shrimp survival and production.     

防病养虾塘水质管理模式

养殖中国对虾的试验塘，以防病养虾塘水质管理模式加以管理，养殖周期为８７－１５９天，取得较好的经济效益。虾塘水的主要理化因子基本符合要求，ＮＨ３－Ｎｔ〈１ｍｇ／，０．００５ｍｇ／Ｌ≤ＮＯ２≤０．０１６ｍｇ／Ｌ，溶氧处于过饱和状态，ＣＯＤＭｎ〈１２ｍｇ／Ｌ，池塘单位水柱毛产氧量约为７ｇ（ｍ＾２．ｄ），总耗氧量约为５－１１ｇ／（Ｌ．ｄ），并借助增氧机补足池水所缺需氧量。     

Heritability of reproductive traits and genetic correlations with growth in the black tiger prawn Penaeus monodon reared in tanks

Captive-reared broodstock of the black tiger prawn (Penaeus monodon) have exhibited poor reproductive performance limiting attempts to domesticate this species. The potential for improved reproductive performance was assessed by determining heritabilities of four measures of reproductive performance, their genetic correlations with each other and with growth rate and weight at age. Heritability estimates (h² ± S.E.) obtained from √ (days to spawn), √ (egg number), √ (nauplii number) and arcsin√ (proportion hatched) were 0.47 ± 0.15, 0.41 ± 0.18, 0.27 ± 0.16, and 0.18 ± 0.16, respectively. Estimates of genetic correlations between reproductive traits and weight at age, or growth rate were less than 0.5 except for √ (egg number) and weight at 54 weeks (0.93 ± 0.19) and √ (egg number) and 16–54-week growth (0.63 ± 0.29).     

Pigment deficiency correction in shrimp broodstock using Spirulina as a carotenoid source

In this study, an effort was made to cure the pigment deficiency syndrome (PDS) exhibited by pond‐reared Fenneropenaeus indicus (H.Milne Edwards) broodstock, using the blue‐green algae Spirulina as a carotenoid source in diet. The PDS symptoms included bleaching of ovary and reduced early larval performance. Biochemical analysis indicated reduced quantity of total carotenoids in egg and nauplii from the females with PDS. When PDS females were fed 30 g kg^?1Spirulina‐supplemented diet immediately after the appearance of symptoms, the egg and nauplii carotenoid values improved gradually and attained the pre‐PDS values after a minimum of 4 week period. Spirulina supplementation also improved significantly (P < 0.05) the spawn quality (hatch per spawn, nauplii per spawn, nauplii viability) and larval quality indices (P < 0.05). The egg and nauplii protein values exhibited a trend similar to carotenoids, confirming the influence of latter on protein stability previously reported by researchers. The study confirms the bioavailability of carotenoids from Spirulina to shrimp broodstock and recommends its regular inclusion in diet to preclude carotenoid deficiency‐related problems in shrimp hatcheries.     

Growth performance of blue shrimp, Litopenaeus stylirostris in self-cleaning microcosm tanks

Outdoor microcosm tanks were used to grow the penaeid blue shrimp, Litopenaeus stylirostris, in Brunei Darussalam. The tanks were cylindrical, free standing fiber glass tanks of 1827 L water holding capacity and had a self-cleaning mechanism. In three eight-week feeding trials, juvenile shrimp of 0.9–4.3 g were stocked at a density of 28 shrimp/m². At the end of each trial, survival rates exceeded 80%. Growth rates ranged from 1.19 to 2.46 g/week. Water quality remained stable and within suitable ranges for L. stylirostris growth in all trials. The tanks had algae and bacterial floc developing within a few days of starting the trials. Fourteen commercial shrimp feeds, each containing more than 40% crude protein, were tested in the trials. In spite of the presence of natural food organisms, significant feed-related differences among treatments were found in each trial. In conclusion, microcosm tanks support excellent growth and survival of L. stylirostris and are appropriate for conducting trials to evaluate feeds for pond growout.     

3种微生物制剂调控工厂化对虾养殖水质的研究

研究了地衣芽孢杆菌(Bacillus licheniformis,BL)、荚膜红假单胞菌(Rhodopseudomonas capsulate,RC)和乳酸杆菌(Lactobacillusspp.,LB)对凡纳滨对虾(Litopenaeus vannamei)工厂化养殖水质的影响。结果表明,施用不同微生物制剂组合,其作用效果各不相同。各组微生物制剂均能降低水体中氨氮,地衣芽孢杆菌 荚膜红假单胞菌(G1)组、地衣芽孢杆菌 荚膜红假单胞菌(G2)和地衣芽孢杆菌 乳酸杆菌(G3)组NH4 的相对降解率为62%、63%和65%;前21d,G2组降低NH4 效果最明显,相对降解率为79%。在降低NO2-方面,G1组的效果较好,相对降解率为46%;但各处理组对于PO34-、COD均无显著效果。相对而言,G1组改善养殖水质的效果最好。实验中微生物制剂的不同施用频率对水质的净化没有明显的差别。各项水质指标均随养殖时间的推移呈现不断上升的趋势。     

The effects of commercial bacterial products on macrobenthos community in shrimp culture ponds

This study was conducted to assess the effects of commercial bacterialproducts on the composition and abundance of macrobenthos in commercial tigerprawn Penaeus monodon culture ponds. Nine brackishwaterponds, with an area of 0.5 ha each were used for this study. Twocommercial bacterial products (T₁ and T₂) were applied inthree ponds each. Another three ponds served as the control. Sediment samplesfrom all ponds were analyzed for the nitrogen, phosphorus and organic mattercontents, and macrobenthos. Total nitrogen and total carbon concentrations inthe sediments of T₁ were significantly lower (p< 0.05) thanT₂. In addition, total nitrogen in T₁ pond sediment wassignificantly lower (p < 0.05) than the control towardsthe end of the culture period. There were no significant differences in totalphosphorus amongst treatments. The study revealed the presence of four majorgroups of macrobenthos in all treatments; gastropods, bivalve, polychaetes andinsect larvae. The total density of macrobenthos in all ponds, exceptgastropods, showed a decreasing trend with the progress in culture indicatingeither predation by shrimp or deterioration of pond bottom. T₁ hadsignificantly (p < 0.05) higher density of polychaetescompared to the T₂ and the control.     

Water quality and phytoplankton stability in response to application frequency of bioaugmentation agent in shrimp ponds

Splitting the recommended weekly dosage of bioaugmentation agent and applied at varying frequencies were tested to determine its effects on water quality and phytoplankton stability in tiger shrimp (Penaeus monodon) grow-out ponds. The supplier's recommended weekly dosage was followed but at varying application frequencies namely: once per week, twice per week, and four times per week for the duration of 120 culture days. A treatment with no bioaugmentation was added as control. Results showed that ammonia and particulate organic matter levels were significantly lower and plankton die-off was greatly minimized if the product was applied four times a week application compared with twice a week and weekly applications. Significant reduction in the number of luminous Vibrio species was observed in ponds with more frequent applications than those with less. For shrimp, survival and production were significantly higher in ponds with twice and four times per week applications compared to the weekly application and the control (P < 0.05). Results of this study indicate that splitting the weekly dosage of bioaugmentation agent and applying it four times per week is highly beneficial and ideal for shrimp culture.     

Assessment of standard aeration efficiency of different aerators and its relation to the overall economics in shrimp culture

Aerators are essential for maintaining the dissolved oxygen level in shrimp culture operations. Many types of aerators are promoted as suitable for shrimp culture, but their comparative efficiencies and water circulation patterns are least understood. The aerators viz, modified paddlewheel, Scorpion jet, Venture jet, Wavesurge were evaluated to compare the standard aeration efficiency at different saline conditions with the commonly used paddle wheel. The modified paddlewheel had the highest mean aeration efficiency of 2.018 kg O₂/ kWh at 35‰, followed by the paddlewheel with 1.434 kg O₂/ kWh at 20‰ water salinity. Compared to this, the Scorpion jet and the Wavesurge have shown the highest aeration efficiency of 0.667 and 0.412 kg O₂/ kWh at 20 and 35‰ water salinities, respectively, whereas the Venture jet has given only. The aerator's performance was better at medium water salinities (20 and 35‰) than low (5‰) or high saline (50‰) conditions. In addition to aeration, the paddlewheel aerator has given the maximum water circulation speed 3 ft/sec and coverage distance of 24 m. The cost economics were derived by keeping all other expenses constant except aeration associated costs. The internal rate of returns in shrimp culture with a modified paddle wheel was 127 % compared to the commonly used paddle wheel (120 %). However, the scorpion and wavesurge aerators have shown returns of 47 % and 27 % respectively across the salinities. The selection and sizing of aerators based on the biomass and efficiency of the aeration systems can make considerable headway in decreasing the cost of production and energy use.