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1.
A new oleanane-type triterpenoid saponin with a carbonyl group at C-15, named polybosaponin A (1), together with a known triterpenoid saponin, dehydrosoyasaponin I (2), were isolated from Radix Hedysari. On the basis of one- and two-dimensional NMR and high resolution electrospray ionization mass spectrometry, the structure of 1 were elucidated and the 1H and 13C NMR spectral signals were assigned totally. In addition, the complete assignment of 1H NMR signals and revision of incorrect assignment of 13C NMR signals for 2 were also achieved based on one- and two-dimensional NMR spectrometry. 相似文献
2.
An ultrasonic technique was employed to extract polysaccharides from Ophiopogon japonicus. The ultrasonic extracted polysaccharides (POJ-U) were purified, and POJ-U1a (a homogeneous fraction) was obtained. The structural characteristics of POJ-U1a were investigated by infrared spectra, gas chromatography, high performance liquid chromatography, periodate oxidation, Smith degradation, methylation analysis, gas chromatography–mass spectrometry and nuclear magnetic resonance spectroscopy. The results indicated that the relative molecular weight of POJ-U1a was 4.02 × 103 Da. POJ-U1a was an α-configuration polysaccharide with a highly branched structure, and consisted of pyranoside and funanside. The backbone of POJ-U1a consisted of 1,6-α-d-glucopyranose and 1,3,6-α-d-glucofuranose in the molar ratio of 7:3, while the branched chains were mainly composed of 1,3-α-d-glucopyranose and 1-α-d-glucopyranose in the molar ratio of 1:3. The branched structure of POJ-U1a was proved intuitively by AFM. Significant antioxidant activity of POJ-U1a has been proved as shown by its DPPH radical scavenging, hydrogen radical scavenging and superoxide anion scavenging activities, which indicated that POJ-U1a showed strong antioxidant activity. 相似文献
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Gmelina arborea Roxb. is a fast-growing species and is known to have been used in traditional Indian medicine. Chemical constituents from
the bark have not been reported, although some chemical constituents from part of this plant (heartwood, leaf, and root) are
known. In this study, the bark meal was successively extracted with acetone and methanol. Fractionation of the acetone extract
with n-hexane, diethyl ether, and ethyl acetate and subsequent chromatographic separation of the fractions led to the isolation
of four compounds. The diethyl ether-soluble fraction yielded tyrosol [2-(4-hydroxyphenyl)ethanol] (1); (+)-balanophonin (2), an 8-5′ neolignan, with opposite optical rotation to known (−)-balanophonin; and gmelinol (3), a known lignan. The ethyl acetate-soluble fraction afforded a new phenylethanoid glycoside to the best of our knowledge,
which was identified as (−)-p-hydroxyphenylethyl[5′″-O-(3,4-dimethoxycinnamoyl)-β-d-apiofuranosyl(1′" → 6′)]-β-d-glucopyranoside (4). From the methanol extract, two known compounds, 2,6-dimethoxy-p-benzoquinone (5) and 3,4,5-trimethoxyphenol (6), were isolated and identified. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay of the identifi ed
compounds indicated that 3,4,5-trimethoxyphenol (6) exhibited moderate activity.
Part of this report was presented at the 57th and 58th Annual Meetings of the Japan Wood Research Society, Hiroshima and Tsukuba,
August 2007 and March 2008, respectively 相似文献
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Ultrasonic extraction (UE) was employed for the extraction of bamboo leaf polysaccharides (BLP). The influential parameters of UE procedure including extraction time, ultrasonic power and solid/liquid ratio were optimized by orthogonal experiments. DEAE-cellulose col- umn chromatography was applied to purify BLP and then the radical scavenging activity of BLP was also evaluated. Optimal extraction conditions were: extraction time of 15 min, ultrasonic power of 300 W, and solid/liquid ratio of 1:15. Four kinds of polysaccharides were obtained by DEAE-cellulose column chromatography; the maximum superoxide radical scavenging rate (20.4%) of BLP was inferior to that of vitamin C (VC, the control) and the hydroxyl radical scavenging rate (50%) was equivalent to that of VC. 相似文献
7.
为给鸭儿芹的综合利用提供科学依据,以鸭儿芹茎、叶为原料,采用水蒸气蒸馏法提取鸭儿芹精油,利用气相色谱-质谱联用技术(GC-MS)对鸭儿芹精油成分进行了分析,利用DPPH、ABTS自由基清除能力试验与FRAP试验及打孔法抑菌试验对其精油的抗氧化抗菌活性进行了分析。结果表明:(1)精油成分方面,从鸭儿芹精油中共分离出32个峰,占总离子峰的93.75%,共鉴定出30种化合物,其主要成分分别为α-芹子烯(42.01%)、β-芹子烯(19.86%)、(Z)-β-金合欢烯(12.76%)、镰叶芹醇(4.16%)、α-没药醇(3.93%)等;(2)抗氧化能力方面,当鸭儿芹精油浓度由19μg/m L上升到38μg/m L时,DPPH反应液中Trolox的当量由0.003μmol上升到0.007μmol,ABTS反应液中Trolox的当量由0.069μmol上升到0.074μmol,FRAP反应液中Trolox的当量由0.225μmol上升到0.280μmol,当精油浓度在一定范围内,其DPPH清除能力、ABTS自由基清除能力及铁离子还原抗氧化能力与其浓度间均呈量效关系,且均呈正相关效应,表明鸭儿芹精油具有良好的抗氧化能力;(3)抗菌活性方面,当鸭儿芹精油浓度为1 280 mg/m L时,金黄色葡萄球菌的抑菌圈直径可达7.05 mm,表现出一定的抑菌活性,且其对金黄色葡萄球菌的抑菌效果显著优于其对大肠杆菌的抑菌效果。 相似文献
8.
Euphorbia acanthothamnos, E. macroclada and E. rigida were investigated for their antioxidant activity. The antioxidant potential of extracts of E. acanthothamnos, E. macroclada and E. rigida was evaluated using different complementary antioxidant tests. 相似文献
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《Fitoterapia》2013
The present study aimed at investigating the structural features and antioxidant activities of a polysaccharide fraction (DHP1A) obtained from Dendrobium huoshanense, a precious herb medicine in China. DHP1A mainly consisted of mannose (Man), glucose (Glc) and a trace of galactose (Gal), with a molecular weight of 6700 Da. Its backbone contained (1 → 4)-linked α-D-Glcp, (1 → 6)-linked α-D-Glcp and (1 → 4)-linked β-D-Manp, with a branch of terminal β-D-Galp. The in vitro antioxidant evaluation revealed that DHP1A had a remarkable inhibition effect on the FeCl2-induced lipid peroxidation. Furthermore, DHP1A pretreatment decreased the production of malondialdehyde (MDA), and restored the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), as well as the level of glutathione (GSH) in the livers of CCl4-treated mice. These results suggested that DHP1A was a potential antioxidant component in D. huoshanense. 相似文献
11.
Rie Suzuki Yasuyuki Matsushita Takanori Imai Mariko Sakurai José Manoel Henriques de Jesus Salete Kiyoka Ozaki Zenesio Finger Kazuhiko Fukushima 《Journal of Wood Science》2008,54(2):174-178
The heartwood and sapwood characteristics of 11 Amazonian trees were investigated. Whereas 7 of the specimens had densities greater than 0.7 g/cm3, the heartwood density of ipê amarelo (Tabebuia serratifolia), maçaranduba (Manilkara huberi), cumaru-ferro (Dipteryx odorata), and guarita (Astronium lecointei) exceeded 1.0 g/cm3. Jatobá contained small amounts of Klason lignin and α-cellulose, and large amounts of holocellulose and alkali extract, suggesting that it has a high polysaccharide content that can be dissolved in an alkaline medium. The difference in the syringyl/guaiacyl (S/G) ratios of the samples before and after alkali extraction suggests that alkali extracts contain syringyl-type polyphenols. In all of the samples, the heart-wood methanol extracts were larger in volume than the sapwood methanol extracts, and the sapwood alkali extracts were larger in volume than the heartwood alkali extracts. The antioxidant activities of the methanol and alkali extracts were assayed by measuring the levels of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and super oxide dismutase (SOD)-like activity, respectively. The heart-wood methanol extract of jatobá (Hymenaea courbaril) exhibited the highest level of activity (EC50 = 44 mg/l), which exceeded that of α-tocopherol (EC50 = 48 mg/l), and the heartwood alkali extracts of jatobá and ipê amarelo had high SOD-like activity comparable with red wine. 相似文献
12.
The chemical composition of the essential oil obtained from Grindelia robusta aerial parts from central Italy was analyzed by GC and GC/MS and 45 components were identified. Borneol (15.2%), alpha pinene (10.3%), trans-pinocarveol (7.0%), bornyl acetate (4.5%), limonene (4.3%) were the main components. The antioxidant activity of the essential oil was evaluated using the DPPH and 5-lipoxygenase tests. 相似文献
13.
The antioxidant effect of aqueous methanolic herb extracts of Serratula coronata, S. wolffii and S. tinctoria was investigated using both enzyme-dependent and enzyme-independent systems. The extracts displayed concentration-dependent inhibition of lipid peroxidation. Flavonoids and ecdysteroids present in the extracts were evaluated as antioxidant components. The flavonoid-containing fraction of the herb extract of S. coronata was more effective in lipid peroxidation than the ecdysteroid-containing fraction. This paper also reports the isolation of quercetin 3-O-methyl ether, apigenin, luteolin, quercetin, luteolin 4'beta-D-glucoside and quercetin 4'beta-D-glucoside from S. coronata. 相似文献
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Flavonoids of Helichrysum compactum and their antioxidant and antibacterial activity 总被引:3,自引:0,他引:3
From the capitula of Helichrysum compactum, the flavonoids apigenin, kaempferol, luteolin, naringenin, 3,5-dihydroxy-6,7,8-trimethoxyflavone, kaempferol-3-O-glucoside, luteolin-7-O-glucoside and luteolin-4',7-di-O-glucoside and from the leafy stems apigenin, kaempferol, luteolin, quercetin, apigenin-7-O-glucoside, luteolin-7-O-glucoside, and quercetin-3-O-glucoside were isolated. Extracts of the capitula of H. compactum show antioxidant activity by inhibition of lipid peroxidation and also show antibacterial activity. 相似文献
16.
采用响应面法优化罗汉松总二萜的提取工艺。在单因素试验基础上,采用Box-Behnken中心组合法设计多因素试验,研究提取温度、甲醇浓度、液料比对罗汉松总二萜得率的影响,建立试验工艺参数与提取量之间的数学模型,确立最佳提取工艺。结果表明:罗汉松总二萜的最佳提取工艺参数为:甲醇浓度80.00%、提取温度63.00℃、液料比26∶1(mL/g),此时罗汉松总二萜得率为1.13%。抗氧化活性试验表明,罗汉松总二萜对DPPH自由基、羟自由基、超氧阴离子具有不同的清除能力,最大清除率分别达到了81.71%、66.27%、38.60%。本研究为罗汉松中二萜类物质的研究和开发利用提供参考。 相似文献
17.
Methanol extract of Rhaphidophora pertusa stem was analyzed for its antioxidant (DPPH, reducing power and Fe(3+) metal chelation methods) and antibacterial activities. The extract was found effective against the three antioxidant test models and exhibited strong and moderate antibacterial activity against the tested pathogenic bacteria. 相似文献
18.
Different parts of Costus speciosus were investigated for their polyphenol content and antioxidant activity. 相似文献
19.
David JP Meira M David JM Brandão HN Branco A de Fátima Agra M Barbosa MR de Queiroz LP Giulietti AM 《Fitoterapia》2007,78(3):215-218
Extracts of 32 plants from the Brazilian northeastern semi-arid region called Caatinga were evaluated through DPPH radical scavenging assay, beta-carotene bleaching, and brine shrimp lethality tests (BST). Among the extracts studied Byrsonima cf. gardneriana, Mascagnia coriacea, Cordia globosa, Diodia apiculata and Hypenia salzmannii showed the highest activities in DPPH radical scavenging test. In the beta-carotene bleaching test the highest activities were observed for Passiflora cincinnata, Chamaecrista repens, B. cf. gardneriana, Rollinia leptopetala, Serjania glabrata, Diospyros gaultheriifolia, C. globosa, Mimosa ophtalmocentra, M. coriacea and Lippia cf. microphylla. In contrast, R. leptopetala, Zornia cf. brasiliensis and Leonotis nepetifolia were the most active species in the BST. 相似文献
20.
The phenolic composition of pine bark from a variety of Pinus species was estimated by measuring Klason lignin, acid-soluble lignin, and a 1% NaOH extract. Polyphenol contents of hot
water extracts from pine bark were determined by the Folin-Ciocalteu assay and the vanillin-H2SO4 assay. Among the pine bark varieties investigated, Pinus radiata bark showed the highest polyphenol content and potent antioxidant activity. Pinus rigida bark was also a usable polyphenol-rich source, whereas Pinus densiflora bark had a low yield (5.1%) of hot water extract, although it showed potent antioxidant activity. Correlations between proanthocyanidin
content in pine bark and antioxidant activity were observed. The results suggested that proanthocyanidin was the crucial contributor
to potent antioxidant activity in pine bark.
The publication of this article was made possible by an Emachu Research Fund. The authors are grateful for the fund. 相似文献