Eradication of potato virus X from potato by ribavirin treatment of cultured potato shoot tips

Ribavirin treatment of cultured potato shoot tips was tested as a means of eradicating potato virus X (PVX) from two potato cultivars. Shoot tips were cultured on liquid medium containing 0, 1, 10 or 100μg/ml ribavirin. Cultures were evaluated periodically for viability, and scored for vigor on a relative growth scale. Developed plantlets were assayed for PVX by transmission tests toGomphrena globosa. Ribavirin treatment was phytotoxic at all tested concentrations, and lethal to all cultivars treated at 100 μg/ml. Treatment also delayed plantlet development by up to 2 months at 1 and 10 μg/ml as compared with nontreated controls. PVX assays indicated that 80 and 83% of the plantlets were free of PVX following treatment with 10 μg/ml for cultivars Russet Burbank and Red McClure, respectively. Five and 6% of the plantlets developed from the 1 μg/ml treatment were PVX-free, whereas 0 and 2% of the controls were PVX-free for the same cultivars. Six to 8 months were required to develop plants from shoot-tip cultures treated with 10 μg/ml ribavirin.     

Recruitment of adult Colorado potato beetles inBt-transgenic potato fields

A refuge of conventional potato plants adjacent to Colorado potato beetle (CPB),Leptinotarsa decemlineata (Say)-resistant,Bt-potato (transgenic) plants may reduce opportunity for the development of tolerance to the resistant plants. The refuge strategy was developed on the basis of data available for CPB recruitment in conventional potato fields. This study was undertaken to provide information on CPB recruitment inBt-transgenic potato fields. A marking experiment was conducted over the 2000 and 2001 crop seasons to determine the relative contributions of beetle populations from fields 10 m, 175 to 300 m, and 1200 to 1280 m distant to the pattern of CPB recruitment (immigration) in aBt-potato field. Season-long CPB recruitment in theBt-potato field decreased with source distance in a manner similar to that previously reported in conventional potato fields. Although marked beetles from the 10-m field plot contributed more to the recruitment than the more distant fields, they contributed only 3.3% and 6.6% of the total beetle sightings in theBt-potato field. Therefore, results suggest that a larger acreage of conventional potato fields at some distance from the resistant crop could replace the adjacent designated refuge. Seasonally, beetles from the overwintering sites provided the first recruits to the resistant field. As their contribution declined, the overwintered beetles from the refuge were recruited until the beginning of the summer population. These results suggest that locating the resistant fields close to active overwintering sites will improve the probability that the refuge strategy will be effective by ensuring the presence of recruits at the very beginning of the crop season. The substantially lower recruitment level obtained for summer than for overwintered CPB in resistant and conventional potato fields highlights the need to reconsider the applicability of the refuge strategy for the summer population.     

Reinfection of potato seed stocks with potato virus S and potato virus X in Wisconsin

Reinfection of potato seed stocks with the potato viruses S (PVS) and X (PVX) varied with cultivar, virus, and grower. Rapid recontamination was observed for the cultivars Norgold Russet and Ontario with PVS and for the cultivars LaChipper, Norchip, and Norgold Russet with PVX. Recontamination was low for the cultivars LaChipper and Monona with PVS and for the cultivars Kennebec, Monona, Norland, and Superior with PVX. Survey results suggest that PVS and/or PVX can be eliminated from cultivars which appear to possess field resistance to infection, but that further evaluation of cultivars which are very susceptible to reinfection will be necessary.     

Ultrastructure of potato tubers infected with potato virus X

The ultrastructure of Katahdin tubers infected with potato virus X (PVX) is compared with PVX-free tubers by electron microscopy. Electron-dense globules surrounding the inner periphery of the tonoplast were observed in PVX-infected tubers, while PVX-free tubers did not show such bodies. Other organelles were comparable in PVX-infected and PVX-free tubers     

Insecticide control of the potato tuberworm in late-crop potato foliage

The systemic insecticides, Dasanit^®, carbofuran (Furadan^®), phorate (Thimet^®), aldicarb (Temik^®) and disulfoton (Di-Syston^®) were applied in the planting furrow at 2–3 lb actual/acre for potato tuberworm (Phthorimaea operculella (Zeller)) control in Irish potato foliage. None of these compounds gave entirely satisfactory results. Dasanit was the best with slightly over 80% decrease in the number of mines and was followed closely by carbofuran; all treatments needed to be supplemented by foliage sprays for satisfactory tuberworm protection. Azinphosmethyl (Guthion^®) gave consistently outstanding results as a foliage spray at 0.5 lb/acre with over 99% tuberworm reduction. Other foliar-applied insecticides giving at least 98% tuberworm control included: Monitor^®, monocrotophos (Azodrin^®), phosphamidon, Orthene^®, Torak^®, methomyl (Lannate^®) and Dursban^® (all at 0.5 lb/acre) and Phosvel^® (1 lb/acre).     

种薯切块与小种薯播种栽培效果比较

从节约用种量考虑 ,生产中常采用种薯切块的方法种植马铃薯。但由于消毒不严 ,一些病害易通过切刀传播 ,引起马铃薯发病 ,造成产量下降。在稻田免耕、稻草全程覆盖种植马铃薯时 ,采用小整薯播种不但可以避免病害传播 ,而且还可以起到抗旱保苗 ,保证苗齐苗壮的作用 ,从而提高产量。现将试验结果整理如下 ,供各地参考。一、材料与方法供试品种为东农３０３。４个薯块重５００ｇ的为大薯 ,７个重５００ｇ的为中薯 ,１４个重５００ｇ的为小薯 ,各选取２５ｋｇ作为试验材料。大薯切成４块 ,中薯切成２块 ,小薯不切。切块种薯用草木灰涂抹切口 ,以…     

Inheritance of extreme resistance to potato virus Y in potato

Summary Segregation for extreme resistance to PVY was evaluated in progenies derived from crossing two extremely resistant potato clones with parents differing in resistance. Resistance was evaluated after mechanical inoculation with PVY^O and PVY^N, and after graft inoculation with PVY^O. Biological and serological tests (ELISA) were used for virus detection. The extreme resistance is governed by a single dominant gene, but observed segregations deviated from the expected ratios. Considerable modifying effects were detectable, depending on the potato genotype and virus isolate, for a significant excess of susceptible genotypes was observed in some progenies. Moreover, genotypes with non-parental types of resistance to PVY were observed.