Effects of high-grain diet feeding on fatty acid profiles in milk,blood, muscle,and adipose tissue,and transcriptional expression of lipid-related genes in muscle and adipose tissue of dairy cowsOA

Background High-grain(HG) diets affect lipid metabolism in the liver and mammary tissue of dairy cows, but its effects on muscle and adipose tissue have not been wide evaluated. Thus, the aim of this study is to clarify this issue.Methods Twelve Holstein cows were randomly divided into two groups: conventional diet group(CON, n = 6) and the HG diet group(n = 6). On day 7 of week 4, rumen fluid was sampled to measure p H, milk was sampled to measure components, and blood was sampled to measure bi...     

Effects of rearing system and antibiotic treatment on immune function,gut microbiota and metabolites of broiler chickensOA

Background:In China,cage systems with a high space utilization have gradually replaced ground litter systems,but the disease incidence of chickens in cages is higher.Broilers in the ground litter pens may be stimulated by more environmental microbes during the growth process and show strong immune function and status,but knowledge of which microbes and their metabolites play an immunomodulatory role is still limited.This study aimed to explore the differences and correlations in the immune funct...     

Dietary calcium supplementation promotes the accumulation of intramuscular fatOA

Background: In the livestock industry, intramuscular fat content is a key factor affecting meat quality. Many studies have shown that dietary calcium supplementation is closely related to lipid metabolism. However, few studies have examined the relationship between dietary calcium supplementation and intramuscular fat accumulation.Methods: Here, we used C2C12 cells, C57BL/6 mice(n = 8) and three-way cross-breeding pigs(Duroc×Landrace×Large white)(n = 10) to study the effect of calcium addition o...     

Dietary supplementation of laminarin improves the reproductive performance of sows and the growth of suckling pigletsOA

Background Maternal nutrition is essential in keeping a highly efficient production system in the pig industry. Laminarin has been shown to improve antioxidant capacity, reduce the inflammatory response, and favor the homeostasis of intestinal microbiota. However, the effect of dietary supplementation of laminarin on the reproductive performance of sows and the growth of suckling offspring remains unknown.Methods A total of 40 Landrace × Yorkshire multiparous sows on d 85 of gestation, similar i...     

Effects of dietary coenzyme Q10 supplementation during gestation on the embryonic survival and reproductive performance of high-parity sowsOA

Background Fertility declines in high-parity sows. This study investigated whether parity-dependent declines in embryonic survival and reproductive performance could be restored by dietary coenzyme Q10(Co Q10) supplementation.Methods Two experiments were performed. In Exp. 1, 30 young sows that had completed their 2^nd parity and 30 high-parity sows that had completed their 10^th parity, were fed either a control diet(CON) or a CON diet supplemented with 1 g/kg Co Q10(+ Co Q1...     

Implication and challenges of direct-fed microbial supplementation to improve ruminant production and healthOA

Direct-fed microbials(DFMs) are feed additives containing live naturally existing microbes that can benefit animals’ health and production performance. Due to the banned or strictly limited prophylactic and growth promoting usage of antibiotics, DFMs have been considered as one of antimicrobial alternatives in livestock industry.Microorganisms used as DFMs for ruminants usually consist of bacteria including lactic acid producing bacteria,lactic acid utilizing bacteria and other bacterial groups,...     

Serum metabolic profile and metabolome genome-wide association study in chickenOA

Background Chickens provide globally important livestock products. Understanding the genetic and molecular mechanisms underpinning chicken economic traits is crucial for improving their selective breeding. Influenced by a combination of genetic and environmental factors, metabolites are the ultimate expression of physiological processes and can provide key insights into livestock economic traits. However, the serum metabolite profile and genetic architecture of the metabolome in chickens have no...     

Effects of supplemental xylanase and xylooligosaccharides on production performance and gut health variables of broiler chickensOA

Background: This study evaluated the effects of supplemental xylanase and xylooligosaccharides(XOS) in a cornsoybean meal(SBM)-based diet on growth performance and intestinal health of broilers. A total of 288 day-old chicks(Cobb 500) were allocated to 36 floor pens(8 birds/pen) equally in 9 dietary treatments in a 3 × 3 factorial arrangement. The treatments were combinations of 3 levels of xylanase(0, 0.005% and 0.01% Econase XT) and 3 levels of prebiotics(0, 0.005% and 0.01% XOS) added to basa...     

Genomic patterns of homozygosity and inbreeding depression in MurcianoGranadina goatsOA

Background: Inbreeding depression can adversely affect traits related to fitness, reproduction and productive performance. Although current research suggests that inbreeding levels are generally low in most goat breeds, the impact of inbreeding depression on phenotypes of economic interest has only been investigated in a few studies based on genealogical data.Results: We genotyped 1040 goats with the Goat SNP50 BeadChip. This information was used to estimate different molecular inbreeding coeffi...     

Fecal microbiota dynamics and its relationship to diarrhea and health in dairy calvesOA

Background: Diarrhea is a major cause of morbidity and mortality in young calves, resulting in considerable economic loss for dairy farms. To determine if some gut microbes might have resistance to dysbiotic process with calf diarrhea by dictating the microbial co-occurrence patterns from birth to post-weaning, we examined the dynamic development of the gut microbiota and diarrhea status using two animal trials, with the first trial having 14 Holstein dairy calves whose fecal samples were collec...     

Heat stress impacts the multi-domain ruminal microbiota and some of the functional features independent of its effect on feed intake in lactating dairy cowsOA

Background: Heat stress(HS) affects the ruminal microbiota and decreases the lactation performance of dairy cows. Because HS decreases feed intake, the results of previous studies were confounded by the effect of HS on feed intake. This study examined the direct effect of HS on the ruminal microbiota using lactating Holstein cows that were pair-fed and housed in environmental chambers in a 2 × 2 crossover design. The cows were pair-fed the same amount of identical total mixed ration to eliminate...     

Microbial and metabolomic mechanisms mediating the effects of dietary inulin and cellulose supplementation on porcine oocyte and uterine developmentOA

Background: Dietary fiber(DF) is often eschewed in swine diet due to its anti-nutritional effects, but DF is attracting growing attention for its reproductive benefits. The objective of this study was to investigate the effects of DF intake level on oocyte maturation and uterine development, to determine the optimal DF intake for gilts, and gain microbial and metabolomic insight into the underlying mechanisms involved.Methods: Seventy-six Landrace × Yorkshire(LY) crossbred replacement gilts of s...     

Effect of dietary magnolia bark extract supplementation in finishing pigs on the oxidative stability of meatOA

Background: Magnolia bark extract(MBE) is a natural supplement with antioxidant, anti-inflammatory, and antimicrobial activities. Its properties suggest that the dietary supplementation in livestock could improve the quality of products. Therefore, the aim of this study was to investigate, for the first time, the effect of dietary MBE supplementation(0.33 mg/kg) in finishing pigs on the oxidative stability of meat. Oxidative stability is of paramount importance for pork, as it affects storage, r...     

Single-cell transcriptomic atlas of goat ovarian agingOA

Background The ovaries are one of the first organs that undergo degenerative changes earlier in the aging process, and ovarian aging is shown by a decrease in the number and quality of oocytes. However, little is known about the molecular mechanisms of female age-related fertility decline in different types of ovarian cells during aging, especially in goats. Therefore, the aim of this study was to reveal the mechanisms driving ovarian aging in goats at single-cell resolution.Results For the firs...     

Stimbiotic supplementation modulated intestinal inflammatory response and improved broilers performance in an experimentally-induced necrotic enteritis infection modelOA

Background: Two experiments were conducted to establish an optimal NE challenge model and evaluate the efficacy of stimbiotic(STB) supplementation in necrotic enteritis(NE) challenged broilers. In Exp. 1, a total of 120 Arbor Acres(AA) broilers(45.0 ± 0.21 g) were randomly assigned to 6 treatments in a 3 × 2 factorial arrangement. Vaccine treatments included non-challenge(0), × 10 the recommended dose(× 10) or × 20 the recommended dose(× 20) by the manufacturer. Clostridium perfringens(CP) treat...     

Effects of lactic acid bacteria-fermented formula milk supplementation on ileal microbiota,transcriptomic profile,and mucosal immunity in weaned pigletsOA

Background:Lactic acid bacteria(LAB) participating in milk fermentation naturally release and enrich the fermented dairy product with a broad range of bioactive metabolites,which has numerous roles in the intestinal health-promoting of the consumer.However,information is lacking regarding the application prospect of LAB fermented milk in the animal industry.This study investigated the effects of lactic acid bacteria-fermented formula milk(LFM) on the growth performance,intestinal immunity,microb...     

Fibroblast growth factor 21 in dairy cows:current knowledge and potential relevanceOA

Fibroblast growth factor 21(FGF21) has been identified as an important regulator of carbohydrate and lipid metabolism, which plays an important role for metabolic regulation, particularly under conditions of energy deprivation or stress conditions. Dairy cows are subjected to a negative energy balance and various kinds of stress particularly during the periparturient phase and during early lactation. It has been shown that the plasma concentration of FGF21 in dairy cows is dramatically increased...     

The significan e of N-carbamoylglutamate in ruminant productionOA

Improving the efficiency and production of grazing ruminants to support food and fiber production, while reducing the environmental footprint and meeting the welfare needs of the animals, is important for sustainable livestock production systems. Development of new technologies that can improve the efficiency of nitrogen(N) utilization in ruminants, and that are effective and safe, has important implications for ruminant livestock production. N-carbomoylglutamate(NCG) is a functional micronutrie...     

Effect of protease supplementation on apparent ileal crude protein and amino acid digestibility of over-processed soybean meals in broilersOA

Background: Nutritional value of proteins in feed ingredients can be negatively affected by hydrothermal processing, which causes large variation in the bioavailability of amino acids(AA) and negatively affects animal productive performance. Supplementation of exogenous proteases could increase the rate of digestion of damaged proteins, thereby increasing overall AA digestibility and bioavailability. The aim was to determine the effect of exogenous protease supplementation on the apparent ileal ...     

Gut health benefit and application of postbiotics in animal productionOA

Gut homeostasis is of importance to host health and imbalance of the gut usually leads to disorders or diseases for both human and animal. Postbiotics have been applied in manipulating of gut health, and utilization of postbiotics threads new lights into the host health. Compared with the application of probiotics, the characteristics such as stability and safety of postbiotics make it a potential alternative to probiotics. Studies have reported the beneficial effects of components derived from ...