Replacing corn with pearl millet (raw and sprouted) with and without enzyme in chickens' diet

An experiment was conducted to compare a commercial corn–soya bean meal diet with a pearl millet (raw and sprouted) diet containing less soya bean meal, alone or in combination with exogenous enzyme, on growth performance and ileal villus development of chicks. Two‐hundred‐and‐forty‐one‐day‐old male broilers (10/pen) were randomly allocated to one of the following dietary treatments: (i) a standard corn–soya bean meal control diet (CTL); (ii) a raw pearl millet‐soya bean meal diet (PM); (iii) a sprouted pearl millet‐soya bean meal diet (SPM); (iv) CTL + exogenous enzymes (CE); (v) PM + exogenous enzymes (PE); and (vi) SPM + exogenous enzymes (SPE) with four replicate pens/treatment. Body weight of birds at day 21 did not differ between those fed the CTL, and SPM and PE diets. In comparison with feeding broilers the CTL diet, feeding the PE and SPM diets caused significant decrease in feed intake, but with equivalent growth and feed efficiency. However, at day 21, feed conversion ratio did not differ between birds fed the CTL diet and those fed the PM, PE and SPM diets. At day 21, broilers fed the PM and PE diets had longer villi (p < 0.05) than those fed the CTL diet. At day 21, villi width was reduced (p < 0.05) by raw pearl millet supplementation than CTL diet. It is concluded that, in comparison with corn, broiler diets formulated with sprouted pearl millet or pearl millet with enzyme require less soya bean meal and can be used to improve growth performance traits and villus development.     

Additivity of values for apparent and standardized ileal digestibility of amino acids in mixed diets fed to growing pigs

The objective of this experiment was to determine whether the digestibility of CP and AA in a mixed diet fed to growing pigs is better predicted when based on standardized ileal digestibility coefficients (SID) or apparent ileal digestibility coefficients (AID). Eight growing pigs (initial BW = 92.1 +/- 3.19 kg) were surgically equipped with a T-cannula in the distal ileum and arranged in an 8 x 8 Latin square design with eight diets and eight periods. Three of the diets contained corn, soybean meal (SBM), or canola meal (CM) as the sole source of CP and AA. Four mixed diets also were formulated using corn and soybean meal (CS); corn and canola meal (CCM); soybean meal and canola meal (SCM); or corn, soybean meal, and canola meal (CSCM). A N-free diet was used to measure the basal ileal endogenous losses (IAAend) of CP and AA. Pigs were fed each of the eight diets during one 7-d period, and ileal digesta were collected during two 10-h periods on d 6 and 7. The AID values were calculated for CP and AA in all diets, except the N-free diet. By correcting the AID for IAAend, the SID for CP and AA in each of the seven protein-containing diets were calculated. As expected, the AID for CP and the majority of AA were greater in SBM than in corn and CM (P < 0.05); however, the SID for CP and most AA did not differ between corn and SBM. For the majority of the AA, SID were less (P < 0.05) in CM than in the other two ingredients. Using the AID and the SID that were measured for CP and AA in corn, SBM, and CM, the AID and the SID in the four mixed diets were predicted and compared with the measured values for these diets. For the three mixed diets containing corn, the measured AID for CP and most AA were greater (P < 0.05) than the predicted AID, but with a few exceptions, no differences between predicted and measured values for SID were observed. For the diet based on SCM, there were no differences between predicted and measured values regardless of the procedure used, except for the AID of Ser. The results of this experiment demonstrate that the digestibility coefficients for a mixed diet containing low-protein feed ingredients, such as corn, are more accurately predicted using SID than AID.     

Nutritive characteristics of pearl millet grain in beef cattle diets

Two trials were conducted to compare pearl millet grain with corn and grain sorghum in cattle diets. Grain portions of diets in metabolism and feedlot experiments contained 73% corn and 6% soybean meal (C); 76.2% grain sorghum and 2.8% soybean meal (GS); and 79% pearl millet (PM). In the metabolism trial (replicated 3 x 3 latin squares; six steers), apparent digestibilities of DM and OM were higher (P less than .05) for C than for GS or PM diets. Ether extract and CP digestibilities were higher (P less than .05) for C and PM than GS. Dietary TDN was higher (P less than .05) for C compared with GS or PM diets. Fecal N was higher (P less than .05) for GS than for C or PM, urinary N was higher (P less than .05) for PM than C or GS, but retained N was similar (P greater than .05) for C, GS and PM diets. In a 90-d feedlot trial (18 individually fed heifers), a trend was observed for higher (P less than .20) ADG on C compared with PM; however, feed/gain was similar for C, GS and PM diets (8.2, 9.1 and 8.5 kg feed/kg gain, respectively). Ruminal fluid acetate:propionate ratios were lower (P less than .05) at 3.5 and 7 h postfeeding for PM compared with C or GS diets on d 83. Pearl millet grain may be used as a feed grain for beef cattle, but diets should be formulated to efficiently utilize the high quantity of protein (about 14% of DM) in this grain as a substitute for supplemental protein.     

Calcium, phosphorus, and amino acid digestibility in low-phytate corn, normal corn, and soybean meal by growing pigs

Nine growing barrows were equipped with a T-cannula in the distal ileum and used to determine apparent ileal (AID) and apparent total-tract digestibility (ATTD) coefficients of Ca and P in low-phytate corn, normal corn, soybean meal, and in diets where soybean meal was mixed with low-phytate corn or normal corn. The AID and the standardized ileal digestibility coefficients (SID) of CP and AA also were determined. The animals (initial BW = 29.3 +/- 1 kg) were allotted to a 9 x 9 Latin square with nine diets and nine periods. Three diets contained low-phytate corn, normal corn, and soybean meal as their sole source of CP, AA, Ca, and P, respectively. Three additional diets were identical to these diets except that limestone and monosodium phosphate were added. Two diets contained low-phytate corn or normal corn and soybean meal, limestone, and monosodium phosphate, and the final diet was a N-free diet. The AID and ATTD of Ca were higher (P < 0.05) for low-phytate corn than for normal corn (70.0 and 69.1% vs. 47.4 and 49.6%, respectively). The AID and ATTD for Ca in soybean meal (50.9 and 46.7%, respectively) did not differ from values for normal corn but were lower (P < 0.05) than for low-phytate corn. The AID and ATTD for P from low-phytate corn (56.5 and 54.5%, respectively) were greater (P < 0.05) than from normal corn (28.3 and 28.8%, respectively), whereas soybean meal had intermediate AID and ATTD for P (37.2 and 38.0%, respectively). The AID and ATTD of P increased (P < 0.05) when monosodium phosphate was added to normal corn (44.9 and 49.8%, respectively) and soybean meal (49.6 and 46.2%, respectively), but adding monosodium phosphate to low-phytate corn, did not alter either AID (49.7%) or ATTD (50.7%) of P. No differences between AID and ATTD for Ca or P within the same diet were observed. The AID of Arg, Asp, Gly, Ile, Lys, Phe, Thr, and Val were greater (P < 0.05) in low-phytate corn than in normal corn. The AID of all AA in soybean meal were greater (P < 0.05) than in both types of corn, with the exception of Ala, Cys, Leu, and Met. The SID of Lys, Phe, and Thr were higher (P < 0.05) in low-phytate corn than in normal corn. Because low-phytate corn has a higher digestibility of Ca and P, less inorganic Ca and P need to be supplemented to diets containing low-phytate corn than to those containing normal corn, and P excretion may be decreased when low-phytate corn is used in the diet.     

Effect of phytase supplementation to diets for weanling pigs on the digestibilities of crude protein, amino acids, and energy

Four experiments were conducted with weanling pigs fitted with a simple T-cannula at the distal ileum, to determine the effect of phytase supplementation to four diets on the apparent ileal digestibilities (AID) of CP and AA, and the apparent total-tract digestibilities (ATTD) of CP and DE. Phytase (Natuphos, DSM Food Specialties, Delft, The Netherlands) was supplemented at rates of 0, 500 or 1,000 FTU/kg to the four diets. A 20% CP (as-fed basis) corn-soybean meal diet was used in Exp. 1; a 20% CP wheat-soybean meal diet in Exp. 2; a 20% CP wheat-soybean meal-canola meal diet in Exp. 3; and a 19% CP barley-peas-canola meal diet in Exp. 4. In each experiment, six barrows, fitted with a simple T-cannula at the distal ileum, were fed the basal plus phytase-supplemented diets according to a repeated 3 x 3 Latin square design. Each experimental period comprised 14 d. The piglets were at fed 0800 and 2000 daily, equal amounts for each meal, at a daily rate of at least 2.4 times the maintenance requirement for ME. Feces were collected from 0800 on d 8 until 0800 on d 12 of each experimental period. Ileal digesta were collected from 0800 to 2000 on d 12, 13, and 14. Chromic oxide was used as the digestibility marker. The average initial and final BW (average of all experiments) were 7.9 and 16.5 kg, respectively. Phytase supplementation did not improve the AID of CP and AA in Exp. 1, 2, and 4; however, there were improvements (P < 0.05) or tendencies (P < 0.10) toward improvements in the AID of CP and AA or the ATTD of CP and the content of DE with phytase supplementation in Exp. 3. These results suggest that the AA response factor to microbial phytase supplementation depends on diet composition.     

Influence of guanidination on apparent ileal digestibility of amino acids in pigs fed diets with soybean meal, rapeseed meal or peas as a protein source

The objective of this study was to determine the effect of guanidination, the conversion of dietary lysine to homoarginine, on the apparent ileal digestibilities (AID) of amino acids (AA) from 3 protein sources in diets for pigs. Six barrows, initial body weight of 18 ± 1.5 kg, fitted with a simple T-cannula at the distal ileum, were fed one of three corn starch-based diets that contained 18% crude protein (CP) from soybean meal, rapeseed meal or peas for an experimental period of 10 d according to a repeated 3 × 3 Latin square design. On d 9 of each experimental period, the pigs were fed one meal of the guanidinated test meals, which corresponded to their respective diets. In the test meals, 50% of the protein source in each diet was replaced, on an equal weight basis, with a guanidinated source. Guanidination increased the content of CP by 190, 116 and 28 g kg^− 1 for soybean meal, rapeseed meal and peas, respectively. With the exception of lysine, the AID of CP and AA were 2.2 to 8.2 percentage units (pu) higher in the test meal than in the soybean meal diet. Other than arginine and isoleucine, the differences were significant (P < 0.05) for all indispensable AA. With the exception of lysine, the AID of CP and AA were 3.8 to 11.9 pu higher in the test meal than in the diet with peas. The differences were significant (P < 0.05) for all indispensable AA. Other than lysine, glycine and proline, the AID of CP and AA were slightly higher, 0.1 to 4.3 pu, in the test meal than in the rapeseed meal diet. Of the indispensable AA, the differences were significant (P < 0.05) for arginine, leucine and phenylalanine. The AID of lysine was 10.9 pu lower (P < 0.05) in the test meal than in the rapeseed meal diet, 61.5 vs. 72.4%, indicating that guanidination of rapeseed meal was not uniform and random. The increase in the AID of CP and AA after guanidination may result from solubilisation of protein and degradation of antinutritional factors such as fiber, trypsin inhibitors and tannins.     

Effect of feed intake on endogenous losses and amino acid and energy digestibility by growing pigs

An experiment was conducted to determine the effect of feed intake (FI) on endogenous losses and the digestibility of CP and AA by growing pigs. Six growing barrows (initial BW = 70.3 kg) had a T-cannula installed in the distal ileum and were used in a 6 x 6 Latin square design. A soybean meal cornstarch-based diet and a N-free diet were formulated. Chromic oxide (0.25%; as-fed basis) was included in both diets as an inert marker. Each diet was provided at three different levels of FI. Feed intake level 1 was equal to the estimated energy requirement for maintenance of the pigs, whereas levels 2 and 3 were two or three times this amount, respectively. Each experimental period lasted 7 d. The initial 4 d of each period comprised an adaptation period to the experimental diets. On d 5, fecal samples were collected, while ileal digesta were collected during two 10-h periods on d 6 and 7. Between each experimental period, a corn-soybean meal-based diet (16% CP) was fed to all pigs for 7 d. The basal ileal endogenous losses (IAA(end)) of CP and AA were measured for each level of FI from pigs fed the N-free diet. Likewise, the apparent (AID) and standardized (SID) ileal digestibility coefficients for CP and AA in soybean meal were calculated for each level of FI. The total-tract digestibility coefficient of energy in the soybean meal-based diet also was calculated. The AID for CP and all indispensable AA except Lys, Met, and Thr increased (P < or =0.055) as FI increased. The IAA(end) of CP and all AA except Pro decreased linearly (P < 0.05) as FI increased when expressed as g/kg of DMI; however, the total daily IAA(end) increased as FI increased (linear, P < or =0.056) for all AA, except for Phe, Thr, Trp, Val, Cys, Gly, and Ser. The AA composition (% of CP) of endogenous protein was not affected by the level of FI, except for Arg, Thr, Pro, and Ser. As FI increased, the SID decreased linearly (P < 0.04) for CP and all AA, except Arg, Trp, Asp, Pro, and Tyr. The total-tract digestibility of energy was not influenced by the FI level. These results demonstrate that the FI level significantly influenced AID, SID, and IAA(end) for CP and AA. Therefore, pigs used to measure AA digestibility coefficients and IAA(end) should be fed at a level that is close to what is used under commercial conditions.     

Apparent and standardized ileal digestibilities of amino acids for pigs fed corn- and soybean meal-based diets at varying crude protein levels

The objective of this study was to determine the effect of CP level in corn- and soybean meal-based diets on apparent (AID) and standardized ileal digestibility (SID) of AA. Six pigs (initial BW, 47.1 ± 1.0 kg) fitted with T-cannula at the distal ileum were fed 6 diets for 6 periods in a 6 × 6 Latin square design. The 6 diets consisted of a nitrogen-free diet and 5 corn- and soybean meal-based diets that contained CP of 68, 105, 141, 177, and 214 g/kg. Each period consisted of a 5-d adjustment period and 2 d of ileal digesta collection for 10 h on each of d 6 and 7. The ratio of corn:soybean meal was fixed at 3 to 2 by weight and cornstarch was added to dilute the CP concentration. Chromic oxide was added at 5 g/kg as an indigestible marker. The results showed basal endogenous loss ranged from 65 mg/kg of DMI for Met to 3,104 mg/kg of DMI for Pro. Proline and Gly (1,053 mg/kg of DMI) were the 2 most abundant AA in endogenous flow and together accounted for approximately 43% of the total endogenous AA flow. Of the basal ileal endogenous CP, total AA accounted for 82%. The AID were 80.9 to 84.7%, 85.1 to 87.4%, 72.9 to 79.5%, and 86.5 to 87.9% for Lys, Met, Thr, and Trp, respectively, with corresponding SID being 86.6 to 89.0%, 87.5 to 90.5%, 82.7 to 88.2%, and 90.2 to 94.6%, respectively, as dietary CP increased from 68 to 214 g/kg. There were linear increases in AID of N, Arg, Gly, Ile, Lys, Ser, Thr, Tyr, and Val (P ≤ 0.05) as CP increased and linear decreases in SID of N and all AA measured in this study except Lys, Met, and Pro (P ≤ 0.05). Both linear and quadratic effects were observed in AID for Pro (P < 0.05). In conclusion, the protein content of corn-soybean meal diets evaluated in the current study affected SID of most indispensable and dispensable AA, excluding Lys, Met, and Pro.     

Effect of dietary soybean oil and soybean protein concentration on the concentration of digestible amino acids in soybean products fed to growing pigs

An experiment was conducted to measure the effect of adding soybean oil to soybean meal (SBM) and soy protein concentrate (SPC) on apparent (AID) and standardized (SID) ileal digestibility of CP and AA by growing pigs. A second objective was to compare AID and SID of AA in a new high-protein variety of full fat soybeans (FFSB) to values obtained in other soybean products. Commercial sources of FFSB (FFSB-CV), SBM, and SPC, and of a new high-protein variety of FFSB (FFSB-HP) were used in the experiment. Four diets were prepared using each soybean product as the sole source of CP and AA in 1 diet. Two additional diets were formulated by adding soybean oil (7.55 and 7.35%, respectively) to the diets containing SBM and SPC. A nitrogen-free diet was also used to measure basal endogenous losses of CP and AA. The 2 sources of FFSB were extruded at 150 degrees C before being used in the experiment. Seven growing barrows (initial BW = 26.2 kg) were prepared with a T-cannula in the distal ileum and allotted to a 7 x 7 Latin square design. Ileal digesta were collected from the pigs on d 6 and 7 of each period. All digesta samples were lyophilized and analyzed for DM, CP, AA, and chromium, and values for AID and SID of CP and AA were calculated. The addition of oil improved (P < 0.05) the SID of most indispensable AA in SBM and SPC. The SID for 6 of the indispensable AA in FFSB-HP were greater (P < 0.05) than in FFSB-CV, and the SID for all indispensable AA except Met was greater (P < 0.05) in FFSB-HP than in SBM. However, the SID for most AA in FFSB-HP was similar to SBM with oil and SPC, but these values were lower (P < 0.05) than in SPC with oil. In conclusion, the addition of oil improved the SID of most AA in SBM and SPC fed to growing pigs, and the SID of AA in FFSB-HP were greater than in SBM and similar to the SID of AA in SBM with oil and in SPC.     

Effect of dietary soybean meal on the microscopic anatomy of the small intestine in the early-weaned pig

Two experiments were conducted to determine the effect of soybean meal in postweaning diets on villus height (VH), villus shape and lamina propria depth (LPD) in the small intestine. Intestinal samples were taken at 25, 50 and 75% of the small intestine's length. The first experiment determined the normal effects of age on these structures in nursing pigs from 9 to 36 d of age. Villus height decreased (P less than .01 at the 50 and 75% locations) and LPD increased (P less than .01 at the 25% location) in a quadratic fashion with increasing age. In the second experiment, pigs were weaned at 21 d of age and fed one of three 20% protein diets: 1) protein from hydrolyzed casein (CAS), 2) soybean meal (SBM) or 3) a conventional corn-soybean meal diet (CSBM). Intestinal samples were taken 0, 3, 6, 9, 12 and 15 d post-weaning. Villus height decreased dramatically the first 3 d postweaning, with a continuing decline until 12 d post-weaning. Averaged over the d 3 to 15 interval, VH were generally greater for pigs fed CAS than for pigs fed CSBM or SBM. Pigs fed SBM or CSBM had deformed villi, and pigs fed SBM had greater (P less than .05) LPD at all intestinal locations, suggesting less mature enterocytes on the villi. These data suggest that feeding high concentrations of soybean meal to the pig post-weaning has a detrimental effect on the small intestine, but this effect was minimized when soybean meal was fed as part of a corn-based diet.     

Effect of phytase supplementation to a low- and a high-phytate diet for growing pigs on the digestibilities of crude protein, amino acids, and energy

Supplementation of microbial phytase usually improves the digestibility and utilization of phosphorus in feedstuffs of plant origin. The effect of phytase supplementation on the digestibilities of AA also has been examined, but the results have been inconsistent. This study was carried out to determine the effect of phytase (Natuphos) supplementation, at a rate of 2,000 phytase units/kg, to two basal diets on the apparent ileal digestibilities (AID) of GE, CP, and AA, and on the apparent total-tract digestibilities (ATTD) of CP and GE. The basal diets contained 18% CP and were formulated (as-fed basis) to contain either a low (0.22%) or high content (0.48%) of phytate P. The high-phytate diet contained 20% rice bran, which is a rich source of phytate and has low intrinsic phytase activity. Eight barrows (average initial BW = 40.6 kg), fitted with a simple T-cannula at the distal ileum, were fed the four diets according to a replicated 4 x 4 Latin square design. The pigs were fed twice daily at 0800 and 2000, equal amounts each meal, at a rate of 2.4 times the daily maintenance requirement for ME. Each experimental period comprised 14 d. Ileal digesta were collected from 0800 to 2000 on d 12, 13, and 14. Feces were collected from 0800 on d 8 until 0800 on d 12. Chromic oxide was used as the digestibility marker. The AID of GE, CP, and AA and the ATTD of CP and GE were less in the high- than in the low-phytate diet (P < 0.01). With the exception of glutamic acid, phytase supplementation did not affect (P > 0.10) the AID of CP and AA. There was no effect (P > 0.05) of phytase on the ATTD of CP and GE. These results show that if a response occurs to phytase supplementation, it is independent of the dietary phytate content.     

Apparent ileal digestibility of amino acids and the digestible and metabolizable energy content of dry extruded-expelled soybean meal and its effects on growth performance of pigs

We conducted three experiments to determine the apparent ileal digestibility of amino acids (Exp. 1), metabolizable and digestible energy (Exp. 2), and feeding value (Exp. 3) of dry extruded-expelled soybean meal with (DEH) or without (DENH) hulls compared with solvent-extracted soybean meal with hulls removed (SBMNH). Soybeans used to produce DEH were unadulterated prior to extrusion, but those used for DENH were dehulled prior to extrusion. In Exp. 1, six nonlittermate barrows (initially 39 kg) were fitted with ileal T-cannulas and used in a replicated 3 x 3 Latin square design digestion trial. Experimental diets (0.80% total lysine) were cornstarch-based and contained soybean meal from one of the three different sources as the sole source of lysine. Apparent ileal digestibilies of nutrients were similar (P > 0.10) for DEH and DENH. Apparent ileal digestibilies of CP, Lys, Ile, Leu, Arg, Phe, and Val were greater (P < 0.05) for DEH and DENH than for SBMNH. In Exp. 2, six barrows (initially 41 kg) were fed three corn-based diets containing 25% of one of the three soybean meal sources. A fourth diet was fed at the end of the trial containing all ingredients except soybean meal, so that energy values of the soybean meal could be determined by difference. Digestible energy and ME contents were similar (P > 0.10) for DEH and DENH and both had greater (P < 0.05) DE and ME contents than SBMNH. In Exp. 3, pigs (n = 216, initially 10.6 +/- 1.3 kg and 35 +/- 3 d of age) were blocked by weight and allotted to six dietary treatments. Corn-soybean meal-based diets (0.95% digestible lysine and 3.44 kcal/g ME) containing DEH or DENH were compared with similar diets containing SBMNH or solvent-extracted soybean meal with hulls (SBMH). In addition, a diet containing a second expelled soybean meal with hulls (ESBM) was compared with a diet containing SBMH and soy oil. Growth performance of pigs fed diets containing DEH or DENH was not different (P > 0.10) than that of pigs fed corresponding diets containing SMBH or SBMNH. Pigs fed ESBM had lower (P < 0.05) ADG and G/F compared with its corresponding SBMH and soy oil diet. In conclusion, DEH and DENH are more digestible than conventional soybean meal and can be successfully used in swine diets.     

Ileal digestibility of amino acids,phosphorus, phytate and energy in pigs fed sorghum‐based diets supplemented with phytase and Pancreatin®

The effects of phytase supplementation on the apparent ileal digestibility (AID) of amino acids (AA) have been inconsistent. Two experiments evaluated the effect of providing a mixture of pancreatic enzymes (Pancreatin^®) to growing pigs fed sorghum–soybean meal diets supplemented with phytase on the AID of AA, energy, and phosphorus (P), as well as the ileal digestibility (ID) of phytate; there were four periods per experiment. In Experiment 1, eight pigs (BW 22.1 ± 1.3 kg) were fitted with a T‐cannula at the distal ileum. Each period consisted of 9 days; 7 days for diet adaptation, and 2 days for digesta collection. Treatments (T) were: (i) basal sorghum–soybean meal diet, (ii) basal diet plus Pancreatin^®, (iii) basal diet plus phytase and (iv) basal diet plus phytase and Pancreatin^®. Phytase increased the digestibilities of phytate and P (p < 0.001), but did not affect the AID of AA and energy (p > 0.10). Except for methionine (p = 0.07), Pancreatin^® did not affect the AID of AA. Phytase and Pancreatin^® did not interact (p > 0.10). Experiment 2 was similar to Experiment 1, but Pancreatin^® was infused into duodenum. Pancreatin^® infusion did not affect the AID of AA (p > 0.10); and tended to reduce (p = 0.09) the AID of lysine. Phytase × Pancreatin^® interactions were not observed (p > 0.10). In conclusion, phytase and Pancreatin^® did not improve the AID of AA in growing pigs fed sorghum–soybean meal diets indicating that phytates did not affect AA digestibility.     

Interrelationship between hypersensitivity to soybean proteins and growth performance in early-weaned pigs.

The objective of this growth trial was to determine the interrelationship between immunological criteria, gut morphology, and performance of starter pigs fed soybean proteins processed by different methods. One hundred twenty-five pigs were orally infused with 6 g/d of either dried skim milk, soybean meal (48% CP), soy protein concentrate, extruded soy protein concentrate, or experimental soy protein concentrate from 7 to 11 d of age and then fed a diet containing the same protein sources from weaning (d 21) to 35 d of age. All pigs were fed a corn-soybean meal diet containing 10% dried whey, 1.25% lysine, and 3% soybean oil for the remaining 21 d of the experiment. Xylose absorption and anti-soy immunoglobulin G (IgG) titers were measured on d 6 postweaning, and skin-fold thickness after intradermal injection of protein extracts was measured on d 7 postweaning. A total of 25 pigs (five pigs/treatment) was euthanatized on d 7 postweaning. Villus height and crypt depth from duodenum samples were measured. These measurements were obtained to elucidate a relationship between the hypersensitivity responses to soybean products and growth performance of baby pigs. Pigs fed diets containing soybean meal had a lower (P less than .05) rate of gain (d 0 to 14) and villus height, higher (P less than .01) serum anti-soy IgG titers, and increased skin-fold thickness (d 6 and 7 postweaning) after intradermal injection compared with those fed dried skim milk. Pigs fed other soy proteins also had lower ADG from d 0 to 14 postweaning; however, pigs fed moist extruded soy protein concentrate tended (P less than .09) to have higher ADG and improved feed utilization when compared with pigs fed soybean meal (d 0 to 14).(ABSTRACT TRUNCATED AT 250 WORDS)     

抗草甘膦转基因豆粕对肉仔鸡肠黏膜免疫的影响

本试验旨在通过研究抗草甘膦转基因豆粕对爱拔益加(AA)肉仔鸡肠黏膜免疫屏障的影响,从黏膜免疫角度评价抗草甘膦转基因豆粕的饲用安全性.选用1日龄AA肉仔鸡360只,随机分成2组,分别饲喂含转基因豆粕和非转基因豆粕的饲粮,试验期21 d.于21 d屠宰取样.采用苏木素-伊红染色法检测肉仔鸡十二指肠、空肠、回肠的小肠绒毛上皮...     

Ileal and total tract nutrient digestibilities and fecal characteristics of dogs as affected by soybean protein inclusion in dry, extruded diets

Plant-based protein sources are generally less variable in chemical composition than animal-based protein sources. However, relatively few data are available on the nutrient digestibilities of plant-based protein sources by companion animals. The effects of including selected soybean protein sources in dog diets on nutrient digestion at the ileum and in the total tract, as well as on fecal characteristics, were evaluated. Six protein sources were used: soybean meal (SBM), Soyafluff 200W (soy flour), Profine F (traditional aqueous-alcohol extracted soy protein concentrate [SPC 1]), Profine E (extruded SPC [SPC 2]), Soyarich I (modified molecular weight SPC [SPC 3]), and poultry meal (PM). All diets were extruded and kibbled. Test ingredients varied in CP and fat contents; however, diets were formulated to be isonitrogenous and isocaloric. Nutrient intakes were similar, except for total dietary fiber (TDF), which was lower (P < 0.01) for dogs fed the PM diet. Apparent ileal digestibilities of DM, OM, fat, and TDF were not different among treatments; however, CP digestibility at the terminal ileum was higher (P < 0.01) for diets containing soy protein sources than for PM. Total tract CP digestibility was greater (P < 0.01) for soy protein-containing diets than for PM. Apparent total tract digestibilities of DM, OM, fat, and TDF were not different among treatments. Apparent amino acid digestibilities at the terminal ileum, excluding methionine, threonine, alanine, and glycine, were higher (P < 0.01) for soy protein-containing diets than for PM. Dogs fed SPC diets had lower (P < 0.01) fecal outputs (g asis feces/g DMI) than dogs fed the SF diet, and dogs fed SBM tended (P < 0.11) to have lower fecal outputs than dogs fed the SF diet. However, dogs fed the PM diet had lower (P < 0.03) fecal outputs than dogs fed SPC-containing diets. Fecal outputs and scores reflected the TDF and nonstructural carbohydrate contents of the soy protein fraction. Soy protein sources are well utilized by the dog prior to the terminal ileum, and SPC offers a viable alternative to PM as a protein source in dry, extruded canine diets.     

Using locally produced millet as a feed ingredient for poultry production in Sub-Saharan Africa

Using locally produced feedstuffs for poultry production in developing countries can greatly lower production costs. Pearl millet is a drought-resistant plant that produces a nutritious grain. Its cultivation in Mali spans thousands of years, but the suitability of the millet grain currently produced in Mali for poultry production is unknown. Therefore, the nutrient composition of 7 different, widely available varieties of pearl millet grown in Mali was assessed before completing experiments in which laying hens or broilers were fed diets containing 0, 14, 28, or 43% whole pearl millet. The 7 varieties of pearl millet grown in different regions of Mali all had protein concentrations that were better than corn and had apparent digestible amino acid coefficients similar to corn. The nitrogen-corrected true metabolizable energy values ranged from 3,395 to 3,738 Kcal/kg on an as-is basis. Egg production and egg weight did not differ between the hens fed the different levels of millet through out a 16-wk experiment. Broilers fed the highest level of pearl millet from 1 to 42 d of age gained less (3.088 versus 2.949 kg) body weight than those fed the control diet. However, body weight gains did not differ between the broilers fed the control diet and the diets containing 14 or 28% whole millet. The results indicate that Malian-grown pearl millet would be an effective feed ingredient for poultry production and that this small seed could be added in whole form into laying hen and broiler diets.     

Effect of protein source and protease addition on performance,blood metabolites and nutrient digestibility of turkeys fed on low-protein diets from 28 to 55 d post hatch

1. The objective of this study was to investigate the effect of a monocomponent protease and dietary inclusion of canola meal (CM) and poultry by-product meal (PBM) on growth performance, carcass characteristics and blood metabolites of turkeys fed on low crude protein (CP) diets from 28 to 55 d post hatch.

2. Experimental treatments included control, maize–soybean meal diet including 258.3 g/kg CP; negative control 1 (NC1), maize–soybean meal diet with reduced CP (232.4 g/kg); NC2, control diet (CP, 258.3 g/kg) including CM (80 g/kg) and PBM (80 g/kg); NC3, maize–soybean meal diet with reduced CP (232.4 g/kg) including CM (80 g/kg) and PBM (80 g/kg). Also, the NC1 + P and NC3 + P diets were created by addition of protease enzyme (30 000 units/kg of diet) to the NC1 and NC3 diets, respectively.

3. The NC3 group had lower body weight gain (BWG) compared to those fed on the control diet, and no improvement with enzyme addition (NC3 + P) was achieved. The protease addition to the NC1 diet (NC1 + P) improved BWG to the level of the control diet. The NC1 group had higher feed conversion ratio (FCR) compared to the control and NC3 + P, but protease addition to the NC1 diet improved FCR.

4. Protease addition to the low CP diets resulted in higher nitrogen (N) retention than in the control and NC2 groups. Also, the NC1 + P and NC3 + P diets increased apparent ileal digestibility (AID) of CP compared to the control group.

5. It was concluded that addition of CM (up to 80 g/kg) and PBM (up to 80 g/kg) to turkey diets had no negative effect on growth performance from 28 to 55 d of age. The NC1 + P group achieved the BWG of the control group which was partially due to increases in N retention and AID of CP, but the NC3 + P group failed to recover the growth losses. This difference implies that the efficacy of the protease may depend upon the protein source in the ration.

     

The effects of thermal treatment of field peas (Pisum sativum L.) on nutrient and energy digestibility by growing pigs

An experiment was conducted to measure the effect of thermal treatment on the digestibility of CP, AA, starch, NDF, ADF, and energy in field peas fed to growing pigs. Five pea-containing diets were formulated. The peas included in these diets were either not heat-treated (control) or extruded at 75, 115, or 155 degrees C or pelleted at 75 degrees C. A N-free diet was also included in the experiment to measure basal endogenous losses of CP and AA. The 6 diets were fed to 6 growing pigs (initial BW: 69.3 +/- 2.9 kg) that were allotted to dietary treatments in a 6 x 6 Latin square design. A T-cannula was installed in the distal ileum of each pig, allowing for the collection of ileal digesta. Each experimental period lasted 9 d; fecal samples were collected on d 6 and 7, and ileal samples were collected on d 8 and 9 of each period. Apparent ileal digestibilities (AID) for CP, AA, starch, and energy and standardized ileal digestibility values (SID) for CP and AA were calculated. Apparent total tract digestibilities (ATTD) for NDF, ADF, starch, and energy were also calculated. As the extrusion temperature increased, the AID and SID for CP and all AA, except Pro, increased (quadratic, P < 0.05). In contrast, except for Arg and Pro, the peas that were pelleted at 75 degrees C had AID and SID for CP and AA that were similar to those obtained for the control peas but less (P < 0.05) than the AID for the peas that were extruded at 75 degrees C. The AID for starch and energy increased (linear, P < 0.001) as the extrusion temperature increased to 155 degrees C (from 89.8 to 95.9% and from 71.5 to 79.0%, respectively), but the AID for starch and energy in the pelleted diet was not different from the AID in the control diet (90.1 vs. 89.8% and 69.1 vs. 71.5%, respectively). The ATTD for starch varied from 98.6 to 99.7% and did not differ among treatments. Likewise, no differences were observed for the ATTD of NDF and ADF. However, the ATTD for energy in the diets increased from 89.0 to 93.3% (linear and quadratic, P < 0.05) as field peas were extruded, and the ATTD for energy in the pelleted diet was also greater (P < 0.05) than that of the control diet (91.6 vs. 89.0%). In conclusion, extrusion of field peas increases the AID of CP, AA, starch, and energy and the ATTD of energy. Pelleting field peas at 75 degrees C does not influence the AID of nutrients or energy but improves the ATTD of energy.     

Soybean hulls as a primary ingredient in forage-free diets for limit-fed growing cattle

In Exp. 1, 300 heifers (260 kg initial BW) were used to compare growth performance of cattle fed forage-free diets containing predominantly soybean hulls with that of cattle receiving roughage- and corn-based diets and to determine whether cattle fed soybean hull-based diets would respond to supplementation with methionine hydroxy analogue (MHA), lipid-coated betaine, or concentrated separator by-product (CSB; a source of betaine). Treatments included 1) a roughage-based diet fed at 2.75% of BW, 2) a corn-based diet fed at 1.5% of BW, 3) a corn-based diet fed at 2.25% of BW, 4) a soybean hull-based diet fed at 1.5% of BW (SH1.5), 5) a soybean hull-based diet fed at 2.25% of BW (SH2.25), 6) SH1.5 top-dressed with 11.4 g/d Alimet (10 g/d MHA), 7) SH2.25 top-dressed with 11.4 g/d Alimet, 8) SH2.25 top-dressed with 7 g/d of a lipid-coated betaine product (4.2 g/d betaine), and 9) SH2.25 top-dressed with 250 g/d CSB (15.5 g/d betaine). Supplemental MHA, betaine, and CSB did not change DMI, ADG, or gain:feed ratio for cattle fed soybean hulls. Heifers fed soybean hull-based diets gained 29% slower (P < 0.05) and had 27% lower gain:feed ratios than heifers fed the corn-based diets. Cattle fed soybean hull-based diets had gains that were lower (P < 0.05) than those of cattle fed the roughage-based diets, but gain:feed ratios were similar because cattle were fed less of the soybean hull-based diets. Roughage-fed cattle had similar gains but 25% lower (P < 0.05) gain:feed ratios than cattle fed the corn-based diets. In Exp. 2, degradation by ruminal microbes of betaine in anhydrous betaine, betaine-HCl, feed-grade betaine, lipid-coated betaine, and CSB was evaluated in vitro using ruminal inocula collected from steers fed a high-grain or high-roughage diet. The roughage diet led to less betaine disappearance than the grain diet. More betaine was degraded from CSB than from other sources, perhaps because sugars provided by CSB stimulated fermentation, but no large differences occurred among the other four sources. Betaine from all sources was extensively degraded, although some betaine may escape ruminal degradation.