稀土元素Ce对爪哇伪枝藻盐胁迫耐受性的影响

荒漠藻类广泛分布于干旱区环境,盐胁迫是影响荒漠藻类生存的重要环境因子,稀土农用及其引起的生态环境问题亦受到广泛关注,为此以一种从荒漠结皮中分离的典型蓝藻——爪哇伪枝藻为材料,在实验室条件下研究盐胁迫和外源稀土元素铈对伪枝藻生理生化特性和细胞结构的影响。试验处理时,先进行铈效应浓度的筛选,然后设置NaCl浓度分别为0（对照）、0.05、0.3 mol.L^-1进行盐胁迫和Ce、Ce＋0.05 mol.L^-1 NaCl、Ce＋0.3 mol.L^-1 NaCl处理,分别测定各种处理下的藻生物量（以叶绿素a表示）、光合活性（Fv/Fm）、藻蓝蛋白含量、胞外多糖含量、膜脂过氧化产物丙二醛含量和伪枝藻素含量以及超微结构的变化。研究结果表明,与对照处理相比,盐胁迫导致伪枝藻生物量和光合活性的显著降低,胞外多糖出现大量积累,藻蓝蛋白和伪枝藻素含量呈现明显下降,同时导致伪枝藻膜脂丙二醛含量的大量增加和藻细胞超微结构的破坏。添加外源铈的盐胁迫处理发现,铈能够促进伪枝藻细胞的生长活性,并对藻细胞的内部结构具有一定的保护作用,而对藻细胞的藻蓝蛋白、伪枝藻素和胞外多糖的影响并不明显。这表明稀土元素铈对于伪枝藻的生长可能具有一定的调节作用,但对于提高伪枝藻的盐胁迫耐受性作用并不显著。研究结果为更好地理解稀土元素对荒漠藻类的生物学效应提供了有意义的参考,可为稀土在荒漠结皮培植中的应用提供指导。     

室内混合藻种荒漠藻结皮培养条件优化研究

为了探索促进荒漠藻结皮形成的最优条件,以民勤地区优势藻具鞘微鞘藻和爪哇伪枝藻为材料,通过室内控制试验,研究了混合藻比例、土壤含水量和接种量对藻结皮培植与发育的影响。结果表明:混合藻种荒漠藻结皮中藻类生物量随接种量和土壤含水量的增加而增加,一定比例的爪哇伪枝藻可显著地促进藻类生物量的积累;混合藻种荒漠藻结皮中藻类生物量积累的最优条件是混合藻比例为8∶2(具鞘微鞘藻∶爪哇伪枝藻)、土壤含水量为10%、接种量为10μg/cm~2(以叶绿素a含量计)。     

干旱人工植被区藻结皮光合固碳的时间效应研究

研究了腾格里沙漠东南缘沙坡头地区不同建植年限(16、21、27、44和52a)人工植被区中发育藻结皮的净光合速率、年固碳量和累计固碳量变化特征,并分析了其与结皮生物学参数(盖度和生物量)和土壤表层0~3 cm有机碳含量的相关关系。结果表明:1)随着人工植被区建植年限的增加,藻结皮的最大净光合速率显著增加,从植被建植16a藻结皮的1.63μmol m-2s-1增加至植被建植52a的2.81μmol m-2s-1;藻结皮的最大光合速率与结皮生物量和结皮盖度呈显著正相关关系;2)藻结皮的年固碳量随植被区建植年限的延长呈指数增加,随着人工植被区建植年限的增加,藻结皮的年固碳量显著增加,从建植16a藻结皮的C 0.2 g m-2a-1增加到52a的C 2.78 g m-2a-1;3)植被区建植后,藻结皮的固碳总量经历两个阶段的变化,建植16a到27a,藻结皮固碳总量在C 2.2~6.2 g m-2,建植44a后,固碳总量增加到C 23.9 g m-2;并且,藻结皮的固碳总量与土壤表层有机碳含量呈显著线性正相关关系。以上研究结果说明,随着人工植被固沙区的演替,藻结皮发育成熟度逐渐提高,其光合固碳能力显著提高,有利于干旱区土壤有机碳的累计。     

库布齐沙漠不同发育类型人工结皮对露水凝结作用的比较研究

露水的形成是干旱、半干旱地区生态系统中重要的环境因子.以库布齐沙漠不同发育类型的人工结皮为研究对象,通过每天野外跟踪观测人工结皮的露水凝结量,比较人工结皮露水凝结量的大小及其凝结能力的差异性.试验以人工蓝藻结皮(ACC)、人工藻结皮(AAC)、人工苔藓结皮(AMC)以及自然苔藓结皮(NMC)和流沙(SS)为材料,并以绒布-平板露水测定法(CPM)为参照,试验时间为42 d.结果表明,CPM法测定的日平均露水凝结量为0.02～0.35 mm,ACC露水凝结量为0.012～0.191 min,AAC为0.032～0.230 mm,AMC为0.048~0.274 mm,NMC为0.056～0.304 mm,SS为0.003～0.083 mm.露水凝结量随结皮的发育状况而存在显著差异,露水凝结量大小依次为NMC>AMC>AAC>ACC>SS.结果还发现.人工结皮的形成明显促进了荒漠地表对露水的吸收和保持,结皮能够比沙表面凝结更多的露水,且结皮的露水千化过程比沙表面更慢.推测结皮藻丝体的发育及其胞外分泌物是结皮凝结较多露水的重要原因,同时依赖于结皮表面的微气候环境.本研究揭示了露水在维系结皮生存中的生态学意义,表明人工结皮具有较好的水土保持功能,同时有利于更好地理解生物结皮对露水的凝结机制与吸收利用情况,并为荒漠化地区有限水源条件下人工结皮利用露水等非降雨型水分进行野外生存提供重要的理论支持和试验依据.     

水分胁迫前的干旱锻炼对小麦光合生理特性的影响

为探讨水分胁迫前的干旱锻炼对小麦光合生理特性的影响,采用水培法,对小麦幼苗进行水分预胁迫、解除胁迫和再胁迫处理,研究了水分预处理对干旱条件下小麦生物量、叶绿素及光合作用的影响。结果表明,经过水分胁迫预处理后的小麦在水分胁迫下根系生长明显加快,有利于吸收利用有限的水分,水分利用效率明显高于未经过预处理的小麦,净光合速率、气孔导度、胞间二氧化碳浓度、蒸腾速率和叶绿素含量的下降幅度均低于未经过预处理的小麦,预处理缓解了干旱对小麦光合生理特性的影响。     

库布齐沙漠东缘荒漠藻人工生物结皮的光谱特性简

为了探明库布齐沙漠东缘荒漠藻人工生物结皮的光谱特性,采用HR1024地物光谱仪,测定研究区包括荒漠藻在内的主要地物光谱数据,分析内蒙古林业科学研究院达拉特旗沙漠综合科学研究站不同年份接种的荒漠藻黑色、绿色结皮及裸沙、沙打旺、油蒿、沙柳的光谱特性及其变化规律.结果表明：1）沙打旺、油蒿、沙柳3种绿色植物光谱曲线与健康植被光谱曲线相近,“红边”位置明显,但荒漠藻黑色、绿色结皮及裸沙的光谱反射率变化比较平缓.2）荒漠藻绿色结皮表现出绿色植物所具有的光谱曲线变化特点;荒漠藻黑色结皮与绿色结皮光谱反射率相比,总的变化趋势为,在350～ 710 nm波段之间,黑色结皮光谱反射率较绿色结皮高,在710～1350nm波段之间,黑色比绿色结皮的光谱反射率低.     

人工藻结皮对旱区农田土壤风蚀的防控效应

[目的]分析人工藻结皮对土壤起沙风速和风蚀速率的影响及其风蚀防控效果,探索人工藻结皮在农田土壤风蚀防控中的可行性,为农田土壤风蚀防控提供新思路。[方法]研究了400,600,800,1 000,1 200 ml/m²共5个藻液接种量对农田土壤藻结皮形成的影响,进而采用风洞试验,研究人工藻结皮对土壤风蚀的防控效应,探讨了该技术应用于农田土壤风蚀防控的可行性。[结果]在室外自然环境条件下,通过接种藻液可形成不同盖度的藻结皮。藻接种量为1 200 ml/m²时,培养14 d盖度可达30%,50 d后,其盖度可达60%以上,藻生物量、结皮厚度是对照的52和9倍,均有显著差异(p<0.05)。人工藻结皮可显著提高农田土壤的起沙风速,降低风蚀速率。当藻结皮盖度为30%左右,起沙风速(9 m/s)较裸土增加44%,风蚀速率较裸土降低80%以上。[结论]通过在农田土壤接种藻液形成藻结皮,可显著改变土壤物理属性,增强土壤抗风蚀性。农田土壤接种藻液形成藻结皮可以作为一种快速、有效的方法应用于农田土壤风蚀防控。     

不同生境对人工结皮发育及表土氮、磷含量及其代谢酶活性的影响

土壤生物结皮对于改善荒漠地区的生态环境和土壤的增肥改良具有重要作用.本研究以库布齐沙漠固定沙丘(迎风坡、坡顶、背风坡和丘间地)披碱草和沙蒿群落下长期发育(8年生)的人工藻结皮为研究对象,测定不同生境下人工结皮对表层土壤氮、磷元素含量以及相关土壤酶活性的影响.结果表明,在不同立地条件下,沙丘顶部结皮叶绿素a和土壤可溶磷含量最高,分别为4.24 mg/g和2.926 mg/kg.丘间地结皮层土壤碱性磷酸酶活性、总磷含量及脲酶活性为最高,迎风坡结皮生物量和土壤碱解氮、速效磷含量以及土壤酶活性明显高于背风坡结皮层土壤.在相同立地条件下,披碱草下结皮生物量、碱解氮、速效磷含量和脲酶及碱性磷酸酶活性显著高于沙蒿下的结皮土壤.本研究表明生境的差异性导致人工结皮发育程度不同,进而对表土氮、磷含量及其代谢酶产生不同影响,人工生物结皮的发育程度与表土氮磷素含量及其代谢酶活性之间存在显著相关性.不同植被群落对生物结皮发育具有不同的作用,披碱草对人工生物结皮影响的正效应高于沙蒿.     

不同水分条件下苔藓结皮光合能力对氮素添加量的响应

[目的]探究苔藓结皮失水过程中光合能力对不同梯度氮添加的响应,为进一步研究苔藓结皮对氮沉降的响应过程以及为干旱、半干旱区荒漠生态系统的管理提供理论依据。[方法]选取毛乌素沙地的优势藓种拟双色真藓(Bryum pachytheca)为研究对象,在控制条件下采用氮添加的模拟试验手段。[结果]苔藓结皮净光合速率在低于0.2g/(m2·a)的氮添加量时受到促进,在高于0.2g/(m2·a)的氮素添加量下,低水分含量时被抑制,高水分含量时会受促进;同时,0.2g/(m2·a)是苔藓结皮能承受的最优氮添加量,此时苔藓结皮的光合固碳能力达到最大,年光合固碳量为对照条件的2倍。[结论]氮沉降引起的氮素增加对于干旱、半干旱区苔藓结皮的光合能力以及固碳潜力具有显著的影响。     

科尔沁沙地生物结皮的土壤种子库特征

通过野外取样和室内分析,初步研究了科尔沁沙地两种生物结皮在干、湿两种处理下土壤种子库种类组成、密度以及物种多样性等特征。结果表明：①科尔沁沙地藻结皮和苔藓结皮土壤种子库的总密度为干旱藻结皮〈湿润藻结皮〈干旱苔藓结皮〈湿润苔藓结皮。两种结皮上种子库的数量存在显著差异,对于干、湿处理的两种结皮,苔藓结皮上种子库的总密度均大于藻结皮上种子库的总密度;②两种生物结皮在干旱、湿润两种处理中共出现了6科、15种植物,种子库的组成以一年生草本植物、中生植物为主,二者百分比均超过76%;③在干旱藻结皮、湿润藻结皮、干旱苔藓结皮和湿润苔藓结皮上,种子库物种多样性表现出湿润处理下的结皮高于干旱处理的结皮。由此表明,在半干旱的科尔沁沙地,水分是限制结皮土壤种子库中种子萌发的主要因子,而生物结皮则通过对水分入渗与再分配的调控作用影响结皮种子库中种子的萌发。     

Effects of drought and salt stresses on man-made cyanobacterial crusts

As a primary successional stage of biological soil crusts (BSCs), cyanobacterial crusts form firstly in the arid and semiarid areas. At the same time, they suffer many stress conditions, such as drought, salt, etc. In this study, we constructed man-made cyanobacterial crusts with Microcoleus vaginatus Gom. and comparatively studied the effects of drought and salt stresses on the crusts. The results showed that crust growth and photosynthetic activity was significantly inhibited by the stress conditions (P < 0.05), and inhibitory effect increased with the increasing stress intensity and treated time. Compared with salt stress, drought completely stopped crust metabolic activity, so the crust biomass was conserved at a higher level, which meant that drought itself might provide the crusts some protection, especially when the crusts simultaneously suffered drought and salt stresses. That is very important for the survival of crusts in the high-salt areas. In addition, to some extent the crusts could adapt to the stress conditions through metabolic adjustment. In our experiment, we found the accumulation of exopolysaccharides (EPS) increased under stress conditions within a certain threshold.     

Influence of dew on biomass and photosystem II activity of cyanobacterial crusts in the Hopq Desert, northwest China

Dew is an important water source for desert organisms in semiarid and arid regions. Both field and laboratory experiments were conducted to investigate the possible roles of dew in growth of biomass and photosynthetic activity within cyanobacterial crust. The cyanobacteria, Microcoleus vaginatus Gom. and Scytonema javanicum (Kütz.) Born et Flah., were begun with stock cultures and sequential mass cultivations, and then the field experiment was performed by inoculating the inocula onto shifting sand for forming cyanobacterial crust during late summer and autumn of 2007 in Hopq Desert, northwest China. Measurements of dew amount and Chlorophyll a content were carried out in order to evaluate the changes in crust biomass following dew. Also, we determined the activity of photosystem II(PSII) within the crust in the laboratory by simulating the desiccation/rehydration process due to dew. Results showed that the average daily dew amount as measured by the cloth-plate method (CPM) was 0.154 mm during fifty-three days and that the crust biomass fluctuated from initial inoculation of 4.3 μg Chlorophyll a cm⁻² sand to 5.8-7.3 μg Chlorophyll a cm⁻² crust when dew acted as the sole water source, and reached a peak value of approximately 8.2 μg Chlorophyll a cm⁻² crust owing to rainfalls. It indicated that there was a highly significant correlation between dew amounts and crust moistures (r = 0.897 or r = 0.882, all P < 0.0001), but not a significant correlation between dew and the biomass (r = 0.246 or r = 0.257, all P > 0.05), and thus concluded that dew might only play a relatively limited role in regulating the crust biomass. Correspondingly, we found that rains significantly facilitated biomass increase of the cyanobacterial crust. Results from the simulative experiment upon rehydration showed that approximately 80% of PSII activity could be achieved within about 50 min after rehydration in the dark and at 5 °C, and only about 20% of the activity was light-temperature dependent. This might mean that dew was crucial for cyanobacterial crust to rapidly activate photosynthetic activity during desiccation and rehydration despite low temperatures and weak light before dawn. It also showed in this study that the cyanobacterial crusts could receive and retain more dew than sand, which depended on microclimatic characteristics and soil properties of the crusts. It may be necessary for us to fully understanding the influence of dew on regulating the growth and activity of cyanobacterial crust, and to soundly evaluate the crust's potential application in fighting desertification because of the available water due to dew.     

毛乌素沙地生物土壤结皮与沙化土地的关系研究

生物土壤结皮在荒漠地区广泛分布,是固沙和促进沙地生态恢复的重要生物因子。利用1989,2002和2006年的Landsat遥感影像,分别通过生物土壤结皮指数和NDVI阈值法,提取了毛乌素沙地生物土壤结皮和沙化土地的空间分布,并分析了二者之间联系。结果表明:(1)研究区1989,2002和2006年结皮面积所占比例分别为54.77%,55.72%和64.29%,表现为持续增加趋势,结皮斑块间连接度在增强,结皮的稳定性进一步巩固。(2)1989—2006年区内沙化土地的程度逐渐减弱,沙化程度综合指数由1989年的3.24下降到2006年的2.65。(3)1989—2006年,中度和重度沙化土地结皮分布范围最为广泛,均在70%以上;极重度沙化土地内的结皮面积表现为持续下降,1989,2002和2006年分别为19.40%,5.76%和2.80%。(4)以1989年沙化土地的格局为基础,1989,2002和2006年极重度沙化土地内生物结皮的数量比例分别为19.40%,33.18%和45.21%,处于上升趋势。     

Relationships between the biomass of algal crusts in fields and their compressive strength

In the desert areas of China investigated by the authors, various biological crusts were predominately associated with three blue-green algal (cyanobacterial) species, Microcoleus vaginatus Gom., Phormidium tenue (Menegh.) Gom. and Scytonema javanicum (Kütz.) Born et Flah. Their biomass and their compressive strength were measured simultaneously in the field in this study. It was also found that the compressive strength of algal crusts was enhanced with the increasing of algal biomass from an undetectable level to a value as high as 9.6 mg g⁻¹ dry soil. However, when the algal biomass decreased, the compressive strength did not descend immediately, but remained relatively steady. The higher the algal biomass became, the thicker were the algal crusts formed. Given the same biomass, the highest compressive strength of man-made algal crusts in fields was found at an algal ratio of 62.5% M. vaginatus, 31.25% P. tenue and 6.25% S. javanicum, and it reached 0.89 kg cm⁻². When the biomass of the crusts increased above the value of 8.16 mg chla g⁻¹ dry soil, the compressive strength would not ascend easily. It indicated that the compressive strength of man-made algal crusts appeared temporarily saturated in the field.     

CARBON FIXATION OF CYANOBACTERIAL–ALGAL CRUSTS AFTER DESERT FIXATION AND ITS IMPLICATION TO SOIL ORGANIC CARBON ACCUMULATION IN DESERT

Enhanced carbon fixation in soil crusts may facilitate the restoration of damaged ecosystems, but this requires greater knowledge of carbon fixation patterns and mechanisms. We measured the net photosynthetic rate (P_n) and estimated annual carbon fixation (ACF) in cyanobacterial–algal crusts after desert fixation in the Tengger Desert, northwestern China. The accumulated carbon fixation since the establishment of a restoration site was also calculated. In addition, stepwise regression analysis was used to study the relation between P_n and ACF and the physicochemical properties of crusts. Results showed that P_n was significantly higher at a more established 51‐year‐old restoration site (1·57 µmol m⁻² s⁻¹) than at a younger 15‐year‐old site (0·92 µmol m⁻² s⁻¹). The ACF also increased significantly with restoration time, but in two temporal phases, a slower ACF phase between 15 and 26 years of restoration (0·28–0·7 gC m⁻² y⁻¹) and a high ACF phase after 43–51 years of restoration (3·3 gC m⁻² y⁻¹). Stepwise regression analysis revealed that P_n was significantly correlated with chlorophyll a and crust cover, whereas ACF was only correlated with crust cover. Accumulated carbon fixation increased from 2·9 gC m⁻² after 15 years to 35·4 gC m⁻² at 51 years following establishment of the restoration site. The accumulated carbon fixation was positively correlated with soil organic carbon content. This study demonstrated that carbon fixation by cyanobacterial–algal crusts increased progressively after desert fixation. Artificial measures, like the establishment of these restoration zones, can facilitate the colonization and development of biological soil crusts and are an effective biological tool for desert soil restoration. Copyright © 2011 John Wiley & Sons, Ltd.     

Microalgal species variation at different successional stages in biological soil crusts of the Gurbantunggut Desert,Northwestern China

Biological soil crusts (BSC), most notably lichen crusts, develop and diversify in the Gurbantunggut Desert, the largest fixed and semi-fixed desert in China. Four different successional stages of BSC, including bare sand, microalgal crusts, lichen crusts, and moss crusts, were selected to determine successional changes in microalgal species composition and biomass and formation of BSC. A 10 × 10-m observation plot was established in an interdune region of the Gurbantunggut Desert and data were collected over an 8-year study period. The main results were: (1) different successional stages of BSC significantly affected the content of soil organic C and total and available N but not the total and available P and K content of soil; (2) composition of microalgal communities differed among the four successional stages; (3) significant differences in microalgal biomass were observed among the four successional stages; (4) bare sand was mainly uncompacted sand gains; (5) filamentous cyanobacteria, particularly Microcoleus vaginatus, were the dominant species in the early phase of crust succession. The presence of fungal mycelium and moss rhizoids prevented water and wind erosion.     

Soil invertebrate activity in biological crusts on tropical inselbergs

Granite inselbergs protrude from forest and savanna in the tropics. They are exposed to harsh climates (alternation of heavy rain and severe drought) and provide little nutrient for plants. Soil animals and humus components were investigated in cyanobacterial crusts close to patches of epilithic vegetation on the surface of the Nouragues inselberg (French Guiana). Three biological crust samples, corresponding to bromeliacean carpets of increasing size (supposed of increasing age), were sampled for faunal and micromorphological studies. Arthropods (mainly mites and insects) were abundant and highly diversified, the more so after enchytraeid worms ate and transformed the cyanobacterial mass. Below the superficial cyanobacterial crust, humus was made of a loose assemblage of enchytraeid faeces where these animals were present, or of a compact assemblage of cyanobacteria and amorphous organic matter where mites were the dominant animal group. Roots abounded in the humified part of the crust. We conclude that soil invertebrates, in particular enchytraeid worms, are important for the accumulation of organic matter on granite outcrops, and so therefore for the encroachment of plant succession.