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1.
Qinghua Wang Jingwei Chen Dexiang Wang Minghui Shen Huilong Ou Jing Zhao Ming Chen Guoliang Yan Jun Chen 《Marine drugs》2021,19(4)
Uncontrolled bleeding is the main cause of mortality from trauma. Collagen has been developed as an important hemostatic material due to its platelet affinity function. A bath sponge skeleton is rich in collagen, also known as spongin. To understand the hemostatic effect of spongin, spongin materials, SX, SFM and SR were prepared from the bath sponge Spongia officinalis, and hemostatic experiments were performed. The SX, SFM and SR were significantly better than the positive control, type I collagen, in shortening the whole blood clotting time in vitro and hemostasis upon rat tail amputation. In a hemostatic experiment of rabbit common carotid artery injury, the hemostatic time and 3 h survival rate of the SFM group were 3.00 ± 1.53 min and 100%, respectively, which are significantly better than those of the commercial hemostat CELOX-A (10.33 ± 1.37 min and 67%, respectively). Additionally, the SFM showed good coagulation effects in platelet-deficient blood and defibrinated blood, while also showing good biocompatibility. Through a variety of tests, we speculated that the hemostatic activity of the SFM is mainly caused by its hyperabsorbency, high affinity to platelets and high effective concentration. Overall, the SFM and spongin derivates could be potential hemostatic agents for uncontrolled bleeding and hemorrhagic diseases caused by deficiency or dysfunction of coagulation factors. 相似文献
2.
Chi-Jen Tai Atallah F. Ahmed Chih-Hua Chao Chia-Hung Yen Tsong-Long Hwang Fang-Rong Chang Yusheng M. Huang Jyh-Horng Sheu 《Marine drugs》2022,20(4)
A polyoxygenated and halogenated labdane, spongianol (1); a polyoxygenated steroid, 3β,5α,9α-trihydroxy-24S-ethylcholest-7-en-6-one (2); a rare seven-membered lactone B ring, (22E,24S)-ergosta-7,22-dien-3β,5α-diol-6,5-olide (3); and an α,β-unsaturated fatty acid, (Z)-3-methyl-9-oxodec-2-enoic acid (4) as well as five known compounds, 10-hydroxykahukuene B (5), pacifenol (6), dysidamide (7), 7,7,7-trichloro-3-hydroxy-2,2,6-trimethyl-4-(4,4,4-trichloro-3-methyl-1-oxobu-tylamino)-heptanoic acid methyl ester (8), and the primary metabolite 2’-deoxynucleoside thymidine (9), have been isolated from the Red Sea sponge Spongia sp. The stereoisomer of 3 was discovered in Ganoderma resinaceum, and metabolites 5 and 6, isolated previously from red algae, were characterized unprecedentedly in the sponge. Compounds 7 and 8 have not been found before in the genus Spongia. Compounds 1–9 were also assayed for cytotoxicity as well as antibacterial and anti-inflammatory activities. 相似文献
3.
In the current study, hemostatic compositions including a combination of chitosan and kaolin have been developed. Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans. Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect following immediate application to injury sites, and thus they have been widely exploited in manufacturing of hemostatic composites. By combining more than one hemostatic agent (i.e., chitosan and kaolin) that act via more than one mechanism, and by utilizing different nanotechnology-based approaches to enhance the surface areas, the capability of the dressing to control bleeding was improved, in terms of amount of blood loss and time to hemostasis. The nanotechnology-based approaches utilized to enhance the effective surface area of the hemostatic agents included the use of Pluronic nanoparticles, and deposition of chitosan micro- and nano-fibers onto the carrier. The developed composites effectively controlled bleeding and significantly improved hemostasis and survival rates in two animal models, rats and rabbits, compared to conventional dressings and QuikClot® Combat Gauze. The composites were well-tolerated as demonstrated by their in vivo biocompatibility and absence of clinical and biochemical changes in the laboratory animals after application of the dressings. 相似文献
4.
Andrea Mencarelli Claudio D’Amore Barbara Renga Sabrina Cipriani Adriana Carino Valentina Sepe Elisa Perissutti Maria Valeria D’Auria Angela Zampella Eleonora Distrutti Stefano Fiorucci 《Marine drugs》2014,12(1):36-53
In the present study we provide evidence that solomonsterol A, a selective pregnane X receptor (PXR) agonist isolated from the marine sponge Theonella swinhoei, exerts anti-inflammatory activity and attenuates systemic inflammation and immune dysfunction in a mouse model of rheumatoid arthritis. Solomonsterol A was effective in protecting against the development of arthritis induced by injecting transgenic mice harboring a humanized PXR, with anti-collagen antibodies (CAIA) with beneficial effects on joint histopathology and local inflammatory response reducing the expression of inflammatory markers (TNFα, IFNγ and IL-17 and chemokines MIP1α and RANTES) in draining lymph nodes. Solomonsterol A rescued mice from systemic inflammation were assessed by measuring arthritis score, CRP and cytokines in the blood. In summary, the present study provides a molecular basis for the regulation of systemic local and systemic immunity by PXR agonists. 相似文献
5.
茶多酚对动物血清血脂和载脂蛋白水平的影响和抗氧化作用 总被引:28,自引:3,他引:25
采用Transansio全自动生化分析仪、自动冲洗旋转式血液流变仪及Chrono -log4 0 0血小板聚集仪分别测定各试验组动物血脂、血粘度、凝血时间、血小板聚集率脂质过氧化物及载脂蛋白水平的作用。结果 2 0 0mg kg-1·d-1茶多酚处理具有明显降低高脂血症小白鼠的血清TC、TG、LDL -C、apoB10 0 ,升高HDL -C (P 相似文献
6.
Fan Yang Yike Zou Ru-Ping Wang Mark T. Hamann Hong-Jun Zhang Wei-Hua Jiao Bing-Nan Han Shao-Jiang Song Hou-Wen Lin 《Marine drugs》2014,12(8):4399-4416
Five new norditerpene endoperoxides, named diacarperoxides H–L (1–5), and a new norditerpene diol, called diacardiol B (6), were isolated from the South China Sea sponge, Diacarnus megaspinorhabdosa. Their structures, including conformations and absolute configurations, were determined by using spectroscopic analyses, computational approaches and chemical degradation. Diacarperoxides H–J (1–3) showed some interesting stereochemical issues, as well as antimalarial activity. 相似文献
7.
Cherie A. Motti Piers Ettinger-Epstein Richard H. Willis Dianne M. Tapiolas 《Marine drugs》2010,8(1):190-199
The viviparous Great Barrier Reef sponge Luffariella variabilis (Poléjaeff 1884) contains a range of secondary metabolites, including manoalide (1) and manoalide monoacetate (3). ESI (+) FTICR-MS accurate mass determination has, for the first time, been used to detected the presence of 3 only in an organic extract of a single L. variabilis larva showing that the parentally produced 3 is sequestered in the larva. As 3 has previously been shown to have antibacterial and quorum sensing inhibition activity, and readily converts to 1, which also exhibits similar activity, it may provide a chemical defence against predation and microbial attack. 相似文献
8.
Hongwei Liu Yuri Mishima Takeshi Fujiwara Hiroshi Nagai Akira Kitazawa Yuji Mine Hisayoshi Kobayashi Xinsheng Yao Junko Yamada Taiko Oda Michio Namikoshi 《Marine drugs》2004,2(4):154-163
A new stereoisomer of an araguspongine/xestospongin alkaloid, named araguspongine M (1), has been isolated together with 12 known compounds, araguspongines B (2) and D (3), dopamine, three galactosyl diacylglycerols, 24-methyl cholesterol, 5,6-dihydrocholesterol, β-sitosterol, and three 5α,8α-epidioxy sterols (11–13), from the marine sponge Neopetrosia exigua (formerly Xestospongia exigua) collected in Palau. The structure of 1 was assigned on the basis of its spectral data analysis. This is the first report on the isolation of dopamine from a marine sponge. This compound may be produced by an endosymbiotic Synechococcus-like cyanobacterium. Compounds 1–3 and 11–13 showed cytotoxicity against HL-60 at IC50’s of 5.5, 5.5, 5.9, 22.4, 9.5, and 9.6 μM, respectively. The possible biosynthesis origin of the isolated metabolites is discussed. 相似文献
9.
Amylopectin Fine Structure and Rice Starch Paste Breakdown 总被引:1,自引:0,他引:1
Ten rice starches with a fairly narrow range of amylose content, but wide variation in RVA pasting curves, were selected to study a possible relationship between amylopectin fine structure and RVA paste viscosity parameters. Amylopectin fine structure was found to significantly correlate with paste breakdown. Proportion of long chains of amylopectin (FrI) was negatively correlated (r=0·84, p<0·01) and proportion of short chains of amylopectin (FrIII) was positive correlated (r=0·89, p<0·001) with paste breakdown. The findings imply that amylopectin fine structure relates to the extent of breakdown of swollen granules and the viscosity after gelatinised starch granule structure is disrupted. The results suggest the possibility that lines can be selected with high proportion of amylopectin long chains for reduced paste breakdown. 相似文献
10.
Ye Yuan Te Li Tingting Wang C. Benjamin Naman Jing Ye Xingxin Wu J. Enrico H. Lazaro Xiaojun Yan Shan He 《Marine drugs》2021,19(10)
LC-MS/MS-based molecular networking facilitated the targeted isolation of a new cyclic hexadepsipeptide, cymodepsipeptide (1), and two known analogues, RF–2691A (2) and RF–2691B (3), from the fungus Cymostachys sp. NBUF082 that was derived from a mesophotic zone Aaptos sponge collected near Apo Island. The constitution and configuration of 1 was elucidated through 1D and 2D NMR-spectroscopy, high resolution mass-spectrometry, and chemical degradations including Marfey’s analysis and chiral HPLC. It was observed that 1 was moderately cytotoxic against CCRF-CEM human acute lymphocytic leukemia cells in vitro with the IC50 value of 9.2 ± 1.1 μM. 相似文献
11.
Chunxiao Sun Ziping Zhang Zilin Ren Liu Yu Huan Zhou Yaxin Han Mudassir Shah Qian Che Guojian Zhang Dehai Li Tianjiao Zhu 《Marine drugs》2020,18(11)
Three new aspochracin-type cyclic tripeptides, sclerotiotides M–O (1–3), together with three known analogues, sclerotiotide L (4), sclerotiotide F (5), and sclerotiotide B (6), were obtained from the ethyl acetate extract of the fungus Aspergillus insulicola HDN151418, which was isolated from an unidentified Antarctica sponge. Spectroscopic and chemical approaches were used to elucidate their structures. The absolute configuration of the side chain in compound 4 was elucidated for the first time. Compounds 1 and 2 showed broad antimicrobial activity against a panel of pathogenic strains, including Bacillus cereus, Proteus species, Mycobacterium phlei, Bacillus subtilis, Vibrio parahemolyticus, Edwardsiella tarda, MRCNS, and MRSA, with MIC values ranging from 1.56 to 25.0 µM. 相似文献
12.
Filipe Natalio Stefanie Wiese Norman Friedrich Peter Werner Muhammad Nawaz Tahir 《Marine drugs》2014,12(8):4659-4676
Iron, as inorganic ion or as oxide, is widely used by biological systems in a myriad of biological functions (e.g., enzymatic, gene activation and/or regulation). In particular, marine organisms containing silica structures—diatoms and sponges—grow preferentially in the presence of iron. Using primary sponge cell culture from S. domuncula–primmorphs—as an in vitro model to study the Demospongiae spiculogenesis, we found the presence of agglomerates 50 nm in diameter exclusively inside sponge specialized cells called sclerocytes. A clear phase/material separation is observed between the agglomerates and the initial stages of intracellular spicule formation. STEM-HRTEM-EDX analysis of the agglomerates (30–100 nm) showed that they are composed of pseudohexagonal nanoparticles between 5 and 15 nm in size, displaying lattice parameters corresponding to hematite (Fe2O3) and mixed iron oxide phases typically attributed to ferritin. Further analysis, using western blotting, inductively coupled plasma mass spectrometry (ICP-MS), sequence alignment analysis, immunostaining and magnetic resonance imaging (MRI), of mature spicule filaments confirm the presence of ferritin within these organic structures. We suggest that S. domuncula can be classified as a dual biomineralizating organism, i.e., within the same cellular structure two distinct biomineralizing processes can occur as a result of the same cellular/metabolic function, spiculogenesis. 相似文献
13.
14.
Giuseppina Chianese Fernando Scala Barbara Calcinai Carlo Cerrano Henny A. Dien Marcel Kaiser Deniz Tasdemir Orazio Taglialatela-Scafati 《Marine drugs》2013,11(9):3297-3308
Chemical analysis of the Indonesian sponge Plakortis cfr. lita afforded two new analogues of the potent trypanocidal agent manadoperoxide B (1), namely 12-isomanadoperoxide B (2) and manadoperoxidic acid B (3). These compounds were isolated along with a new short chain dicarboxylate monoester (4), bearing some interesting relationships with the polyketide endoperoxides found in this sponge. Some semi-synthetic analogues of manadoperoxide B (6–8) were prepared and evaluated for antitrypanosomal activity and cytotoxicity. These studies revealed crucial structure–activity relationships that should be taken into account in the design of optimized and simplified endoperoxyketal trypanocidal agents. 相似文献
15.
Carboxymethylcellulose (CMC) fibers have been successfully prepared from viscose fibers through the process of alkalization-etherification. Parameters including reaction temperature, mass ratio of NaOH to the viscose fibers, and mass ratio of the viscose fibers to ethanol are studied. The degree of substitution (DS) and the inherent viscosity of the CMC fibers are determined. The CMC fibers are characterized by using Fourier transform infrared spectroscopy (FT-IR), 1H-nuclear magnetic resonance spectroscopy (1H-NMR), scanning electron microscopy (SEM), and the X-ray diffraction (XRD). The analysis demonstrates that under the experimental conditions where the reaction temperature is 40 °C, mass ratio of NaOH to the viscose fibers is 2.0, and mass ratio of the viscose fibers to ethanol is 1:15, the obtained CMC fibers possess an appropriate DS, better water-solubility, and lower degree of etching, thus they can be used as absorbable hemostatic fibers. 相似文献
16.
Cao Van Anh Jong Soon Kang Byeoung-Kyu Choi Hwa-Sun Lee Chang-Su Heo Hee Jae Shin 《Marine drugs》2021,19(8)
Ten secondary metabolites, including a new grifolin analog, grifolin B (1); a new homovalencic acid derivative, 12-hydroxyhomovalencic acid (7); and a compound isolated from a natural source for the first time (9), along with seven known compounds, grifolin (2), averantin (3), 7-chloroaverantin (4), 1′-O-methylaverantin (5), 7-hydroxy-2-(2-hydroxypropyl)-5-pentylchromone (6), homovalencic acid (8), and bekeleylactone E (10), were isolated from two fungal strains. The structures of 1–10 were identified by detailed analysis and comparison of their spectroscopic data with literature values. Compounds 9 and 10 showed moderate cytotoxic activity against a panel of cancer cell lines (PC-3, HCT-15, MDA-MB-231, ACHN, NCI-H23, NUGC-3), with the GI50 values ranging from 1.1 µM to 3.6 µM, whereas 1 displayed a weak 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity without cytotoxicity against all tested cell lines. 相似文献
17.
The effect of hydrothermal treatment on the pasting, hydration properties and colour quality of commercial fresh pasta were studied following an Instantaneous Controlled Pressure Drop treatment. This hydrothermal procedure involves a physical modification at high temperature (<144 °C) and restricted moisture content (<30%) during a very short time (15–40 s) followed by a rapid pressure drop to a vacuum (50 mbar). Two process variables (steam pressure level and processing time) were investigated using response surface methodology. Steam pressure level had the greatest effect on hydration and pasting parameters. Increased pressure resulted in elevation of mass ratio between cooked and uncooked pasta (Wi/W0)100 °C and reduction of for all treated pasta. The optimum cooking time of untreated pasta was 7 min (W/W0=2.4), whereas for the same ratio it was less than 1 min for pasta treated at pressures ranging between 2.5 and 3.5 bar. The viscosity at 10 min (V10) increased linearly when the pressure level increased. V10 was 146 cP for untreated pasta whereas it reached 2659 cP for pasta treated at 3.9 bar (144 °C) for 25 s. The cold paste viscosity and setback viscosity decreased when the pressure varied from 1 to 3.9 bar. 相似文献
18.
Yuji Sumii Naoyuki Kotoku Akinori Fukuda Takashi Kawachi Masayoshi Arai Motomasa Kobayashi 《Marine drugs》2015,13(12):7419-7432
Oral dictyoceratin-C (1) and A (2), hypoxia-selective growth inhibitors, showed potent in vivo antitumor effects in mice subcutaneously inoculated with sarcoma S180 cells. Structurally modified analogs were synthesized to assess the structure–activity relationship of the natural compounds 1 and 2 isolated from a marine sponge. Biological evaluation of these analogs showed that the exo-olefin and hydroxyl and methyl ester moieties were important for the hypoxia-selective growth inhibitory activities of 1 and 2. Thus far, only substitution of the methyl ester with propargyl amide in 1 was found to be effective for the synthesis of probe molecules for target identification. 相似文献
19.
Cristiano Di Benedetto Alice Barbaglio Tiziana Martinello Valentina Alongi Dario Fassini Emanuele Cullorà Marco Patruno Francesco Bonasoro Mario Adolfo Barbosa Maria Daniela Candia Carnevali Michela Sugni 《Marine drugs》2014,12(9):4912-4933
Collagen has become a key-molecule in cell culture studies and in the tissue engineering field. Industrially, the principal sources of collagen are calf skin and bones which, however, could be associated to risks of serious disease transmission. In fact, collagen derived from alternative and riskless sources is required, and marine organisms are among the safest and recently exploited ones. Sea urchins possess a circular area of soft tissue surrounding the mouth, the peristomial membrane (PM), mainly composed by mammalian-like collagen. The PM of the edible sea urchin Paracentrotus lividus therefore represents a potential unexploited collagen source, easily obtainable as a food industry waste product. Our results demonstrate that it is possible to extract native collagen fibrils from the PM and produce suitable substrates for in vitro system. The obtained matrices appear as a homogeneous fibrillar network (mean fibril diameter 30–400 nm and mesh < 2 μm) and display remarkable mechanical properties in term of stiffness (146 ± 48 MPa) and viscosity (60.98 ± 52.07 GPa·s). In vitro tests with horse pbMSC show a good biocompatibility in terms of overall cell growth. The obtained results indicate that the sea urchin P. lividus can be a valuable low-cost collagen source for mechanically resistant biomedical devices. 相似文献
20.
The oxidized sodium carboxymethylcellulose (O-CMC) fibers have been successfully synthesized via the oxidation-etherification method. Subsequently, the O-CMC fibers are characterized by Fourier transform infrared (FT-IR), 13C nuclear magnetic resonance (13C-NMR) and X-ray diffraction (XRD). In addition, the viscosity behaviors of the O-CMC aqueous solution have been investigated at five temperatures (25, 30, 40, 50 and 60 °C), six concentrations (30, 35, 40, 45, 50 and 60 kg/m3) and four shear rates (6, 12, 30 and 60 rpm). The results show that the viscosity of the O-CMC aqueous solution increases with increasing concentrations and decreases with increasing temperature. The viscosity of the O-CMC aqueous solution is found to exhibit a dilatant behavior. The dissolution rate in water of the O-CMC fibers is faster than that of the carboxymethylcellulose (CMC) fibers. It could be used as novel absorbable hemostatic fibers in surgery. 相似文献