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1.
Mohammad Reza Naghavi Mariam Hajikram Ali Reza Taleei Mohammad Jafar Aghaei 《Genetic Resources and Crop Evolution》2010,57(3):423-430
Genetic diversity and population genetic structure of Aegilops tauschii in Northern Iran were studied based on nine microsatellite loci. A high level of genetic diversity was observed from the
accessions collected from six regions (provinces). These accessions include 79 samples of the two subspecies (tauschii and strangulata), the intermediate form (among morphologically distinguished subspecies) and ten accessions of Triticum aestivum. The nine microsatellites revealed a total of 141 alleles, with an average of 15.7 alleles per locus. A comparison of the
parameters showing genetic diversity, including the observed heterozygosity (Ho), gene diversity (He) and Shannon’s information index (I) of Ae. tauschii accessions from different provinces in Northern Iran, indicated that subsp. tauschii possesses the highest genetic diversity, followed by intermediate form. Genetic distance between subsp. strangulata and subsp. tauschii was low, confirming high gene flow between these two subspecies. However, intermediate form was more distinct from both of
them. It was also found that the genetic diversity of T. aestivum is obviously lower than that of Ae. tauschii accessions. Moreover, the level of genetic diversity for Gilan, Golestan and Mazanderan provinces was higher than for Ardebil,
Ghazvin and Semnan provinces, suggesting that these regions may provide a readily available source of potentially useful variation
for wheat improvement. 相似文献
2.
Hojjatollah Saeidi Mohammad Reza Rahiminejad Sadeq Vallian J. S. Heslop-Harrison 《Genetic Resources and Crop Evolution》2006,53(7):1477-1484
Microsatellite markers were used to analyse the biodiversity of 57 accessions of different subspecies and varieties of wild
Aegilops tauschii (2n = 2x = 14; D genome) collected across the major areas where it grows in Iran. Levels of diversity were high, with numbers of alleles
averaging 7.3 (ranging up to 12) and polymorphism information contents averaging 0.6591. One accession was notably more similar
to two of the D genome in hexaploid wheats (Triticum aestivum) used as outgroups. Within the Ae. tauschii accessions, no markers were characteristic for taxa or geographical origin, suggesting high gene flow between the subspecies
and varieties, although some groupings, which could be related to geographical origin, were evident. This survey demonstrates
the high diversity present in wild goatgrass in Iran, and indicates that there is value in sampling for useful genes for wheat
breeding. 相似文献
3.
Citrullus colocynthis is a desert plant with a rich history as an important medicinal plant and as a source of valuable oil. Its small seeds appear
in several early Egyptian, Lybian and Near Eastern sites from about 4000 BC. Sequence information deduced from several polymorphic
intergenic cpDNA regions and a relatively large intron (0.6 kb) at the transit sequence of single-copy nuclear gene G3pdh showed clear geographical structure among C. colocynthis accessions. Region specific haplotypes with different nucleotide substitution rates were observed. The highest number of
substitution events occurred in C. colocynthis selections from India and Pakistan, the lowest number in accessions from the Middle Eastern and West Asian region (Iran,
Israel, and Afghanistan). Population level variation at the noncoding cpDNA and G3pdh intron are congruent. Indels at two cpDNA regions and unique nuclear and cpDNA substitutions indicate the direction of migration
from the African continent into the Middle East and the Far East. C. colocynthis collected in Australia showed the highest sequence homology with accessions from Cyprus and Morocco. Divergent lineages are
restricted to portions of the species range. Possible causes of this pattern include restricted migration and gene flow between
regions, and differences in population size of divergent haplotypes based on differential patterns of environmental adaptation. 相似文献
4.
L. Riahi N. Zoghlami K. El-Heit V. Laucou L. Le Cunff J. M. Boursiquot T. Lacombe A. Mliki A. Ghorbel P. This 《Genetic Resources and Crop Evolution》2010,57(2):255-272
Three gene pools representative of Vitis vinifera L. subsp. vinifera (=subsp. sativa Beger) growing in the Maghreb regions (North Africa) from Tunisia (44), Algeria (31) and Morocco (18) and 16 wild grape accessions
(Vitis vinifera L. subsp. sylvestris (Gmelin) Beger) from Tunisia were analysed for genetic diversity and differentiation at twenty nuclear microsatellites markers
distributed throughout the 19 grape chromosomes. 203 alleles with a mean number of 10.15 alleles per locus were observed in
a total of 109 accessions. Genetic diversities were high in all populations with values ranging from 0.6775 (Moroccan cultivars)
to 0.7254 (Tunisian cultivars). F
st pairwise values between cultivated grapevine populations were low but found to be significantly different from zero. High
F
st pairwise values were shown between wild and cultivated compartments. Two parent offspring relationships, two synonyms and
two clones of the same cultivar were detected. The rate of gene flow caused by vegetative dissemination of cultivated grapevine
plants was not sufficient to genetically homogenise the pools of cultivars grown in different regions. The Neighbour Joining
cluster analysis showed a clear separation according to geographical origins for the cultivated grapevines gene pools and
revealed a high dissimilarity between cultivated and wild grapevine. However, three cultivars (Plant d’Ouchtata 1, Plant de
Tabarka 3 and Plant d’Ouchtata 3) are very close to wild accessions and may result from a hybridisation between cultivated
and wild accessions. The high level of differentiation between cultivated and wild accessions indicates that the cultivated
accessions do not derive directly from local wild populations but could mostly correspond to imported materials introduced
from others regions during historical times or derived from crossing between them. 相似文献
5.
Alireza Pour-Aboughadareh Mostafa Mahmoudi Mohammad Moghaddam Jafar Ahmadi Ali Ashraf Mehrabi Seyed Siamak Alavikia 《Genetic Resources and Crop Evolution》2017,64(3):545-556
Genetic erosion in cultivated wheat provides a good reason for studying genetic diversity in crop wild relatives. In the present study, genetic diversity of 32 accessions belonging to T. boeoticum species collected from different parts of Iran were evaluated using 13 morphological traits as well as ten inter-simple sequence repeat primers. Statistical analysis for morphological traits showed significant differences among accessions (except number of fertile tillers and total tillers per plant). In principal component analysis, the first three PCA showed 82.65 % of the total morphological variation. Based on the morphological traits, accessions were separated into two main groups by cluster analysis. In molecular analysis, polymerase chain reactions amplified 105 DNA fragments, out of which, 95 (90.47 %) were polymorphic. From geographic perspective, the accessions sampled from western and southwestern of Iran showed the highest and lowest polymorphism, respectively. However, the maximum values of effective number of alleles (Ne), Nei’s gene diversity (He) and Shannon’s information index (I) was related to accessions collected from NW regions. Also, according to cluster analysis and PCoA plot genetic diversity was not related to geographical distribution. Overall, our results revealed a remarkable level of genetic diversity among studied Iranian T. boeoticum accessions; especially accessions collected from Kermanshah and Lorestan provinces, which can be of interest for future breeding programs. So, conservation of germplasm of these areas is recommended. 相似文献
6.
Togha Jenabi Hojjatollah Saeidi Mohammad Reza Rahiminejad 《Genetic Resources and Crop Evolution》2011,58(4):497-505
The mountain rye Secale strictum is native to the Middle East and is the progenitor of the cultivated rye. Regarding lack of information about the genetic
diversity of this species in Iran, this study was aimed to evaluate its genetic variation and to examine the patterns of diversity
related to the varieties and geography. Fifteen wheat and rye derived microsatellite markers were used to achieve this aim.
High levels of diversity, with an average number of 6.1 alleles per locus (ranging up to 11) and high level polymorphism with
polymorphism rate averaging 0.624 (between populations) and 0.357 (within populations) were observed among 125 individuals
from 19 populations collected from various regions of Iran. The Northwestern populations showed the highest and the Northern
populations showed the lowest polymorphism and diversity. One population of the Northwest of the country was notably closer
in its allele range to the S. cereale accessions used as outgroup. No taxon or geographic specific marker was detected, suggesting high gene flow between the populations,
however some groupings which can be related to the geographic regions and varieties, were evident. The analysis of molecular
variance attributed same portions of genetic diversity to the within and between populations with no significant variation
among different geographic regions. The results of this study indicated that the Iranian genepool of Secale strictum is valuable to search for new useful alleles for crop improvement. 相似文献
7.
Mehraj Abbasov Zeynal Akparov Thomas Gross Sevda Babayeva Vusala Izzatullayeva Elchin Hajiyev Khanbala Rustamov Patrick Gross Mehmet Tekin Taner Akar Shiaoman Chao Robert Brueggeman 《Genetic Resources and Crop Evolution》2018,65(5):1441-1453
Genetic diversity of 139 accessions of diploid Triticum species including Triticum urartu, Triticum boeoticum and Triticum monococcum was studied using 11 SSR (simple sequence repeats) markers. A total of 111 alleles with an average of 10 alleles per locus were detected. The polymorphism information content (PIC) of each SSR marker ranged from 0.30 to 0.90 with an average value of 0.62. Among the three Triticum species T. urartu had the highest number of total alleles (Na?=?81), private alleles (Npa?=?15) and showed higher genetic diversity (Hex?=?0.58; PIC?=?0.54). The genotypes from Turkey exhibited the highest genetic diversity (PIC?=?0.6), while the least diversity was observed among 4 Georgian accessions (PIC?=?0.11). Cluster analysis was able to distinguish 139 wheat accessions at the species level. The highest genetic similarity (GS) was noted between T. boeticum and T. monococcum (GS?=?0.84), and the lowest between T. urartu and T. monococcum (GS?=?0.46). The grouping pattern of the PCoA analysis corresponded with cluster analysis. No significant differences were found in clustering of T. urartu and T. monococcum accessions with respect to their geographic regions, while within T. boeoticum species, accessions from Iran were somewhat associated with their geographical origin and clustered as a close and separate group. The results from our study demonstrated that SSR markers were good enough for further genetic diversity analysis in einkorn wheat species. 相似文献
8.
Yoseph Beyene Anna-Maria Botha Alexander A. Myburg 《Genetic Resources and Crop Evolution》2006,53(8):1579-1588
Over the past three centuries, maize has become adapted to complex environmental conditions in the highlands of Ethiopia.
We analyzed 62 traditional Ethiopian highland maize accessions, using 20 simple sequence repeat (SSR) markers and 15 morphological
traits, to assess genetic diversity and relationships among these accessions and to assess the level of correlation between
phenotypic and genetic distances. The accessions varied significantly for all of the measured morphological traits. The average
number of alleles per locus was 4.9. Pair-wise genetic dissimilarity coefficients ranged from 0.27 to 0.63 with a mean of
0.49. Ward minimum variance cluster analysis showed that accessions collected from the Northern agroecology were distinct
from the Western and Southern agroecologies. However, there was no differentiation between the Western and Southern accessions.
This suggested gene flow between these regions. The relationship between morphological and SSR-based distances was significant
and positive (r = 0.43, p = 0.001). The high genetic diversity observed among these set of accessions, suggests ample opportunity for the development
of improved varieties for different agroecologies of Ethiopia. From conservation perspective, sampling many accessions from
all agroecologies would be an effective way of capturing genetic variation for future collections and conservation. 相似文献
9.
Hojjatollah Saeidi Badradin Ebrahim Sayed Tabatabaei Mehdi Rahimmalek Majid Talebi-Badaf Mohammad Reza Rahiminejad 《Genetic Resources and Crop Evolution》2008,55(8):1231-1238
The diploid goatgrass Aegilops
tauschii is considered the D-genome donor of bread wheat and has probably a centre of diversity in north of Iran. In order to measure
the genetic diversity of and the relationships among different populations, varieties and subspecies belonging to Ae. tauschii in Iran, DNA was extracted from 48 accessions of Ae. tauschii collected across the geographic range of the species in the Country and the genetic diversity was assessed using AFLPs based
on eight PstI/MseI +3 primer pairs resulted in 277 bands, 198 of which were polymorphic. High level polymorphism was detected, with an average
of polymorphism rate of 0.715; relatively low genetic similarity (0.455) between accessions and significant difference between
the lowest (0.179) and the highest genetic similarity (0.817). The Iranian Ae. tauschii populations showed high level of genetic diversity. The populations studied were divided into two groups: one group was mainly
representing Northern populations collected from Southern Caspian Sea shore and the other group was mainly representing Northeast
and Northwest populations. Based on the results of this study, it can be suggested that Ae. tauschii possesses two separate gene-pools in Iran: Northern and Northeastern–Northwestern. Considering the needs for introducing
new characteristics and alleles for wheat improvement purposes, Ae. tauschii Iranian gene-pool is assumed to be of high importance for more investigation in the future. 相似文献
10.
Wei Li Yu-Ming Wei Li-Li Xu An-Jun Liu Yu-Zhen Sheng Zhi-En Pu Guo-Yue Cheng You-Liang Zheng 《Genetic Resources and Crop Evolution》2014,61(6):1105-1119
Variation of high-molecular-weight glutenin subunit (HMW-GS) in 632 wild and cultivated Triticum accessions was investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. A total of 11 alleles of HMW-GS in diploid species, 22 in tetraploid species, and 15 in hexaploid species were detected. Diploid species on Glu-1A locus and tetraploid species Glu-1B locus showed the highest diversity, respectively. Tetraploid species had the highest level of diversity on three Glu-1 loci, followed by hexaploid and diploid, based on Shannon’s information index, Nei’s genetic diversity, and percentage of polymorphic loci. Molecular variance analysis confirmed main variance of HMW-GS within species, regions, and locations, respectively. Variance among species and regions was enhanced gradually with the increase of ploidy. Significant non-random distributions between the phylogenic trees of HMW-GS and the locations of accessions were tested by GenGIS software, indicated that geographic factors played an important role along the different orientations in the spread of Triticum species. We found one original diversified center in diploid what located around Elazig, Malatya, Gaziantep, Urfa, and Kiziltepe in Turkey, and three diversified centers in tetraploid wheat, including Turkey–Armenia–Georgia–Iran, Portugal–Spain, and Ethiopia, respectively, and two diversified adjacent areas between Turkey and Switzerland and around Turkey, Georgia, and Armenia. The original center of diploid species located in southeast Turkey, where the unexpressed 1Ay subunit was mainly distributed in T. urartu, could be one of the candidate regions of polyploidization of Triticum L. The regional distribution of HMW-GS and species also provided geographic evidences for the existence of founder effect on the spread of Triticum species. The present study suggests that integrating genetic diversity with geographic characterization in Triticum could very useful for collection, conservation, and utilization, as well as for research microevolution and domestication. 相似文献
11.
Arvindkumar Salunkhe Shubhada Tamhankar Sujata Tetali Maria Zaharieva David Bonnett Richard Trethowan Satish Misra 《Genetic Resources and Crop Evolution》2013,60(1):165-174
Emmer wheat (Triticum dicoccon Schrank) is still largely cultivated in India, and highly appreciated for the preparation of traditional dishes. Moreover, its nutritional characteristics could justify a development of its cultivation. The perspective of genetic improvement however requires a good knowledge of the genetic diversity existing within the eco-geographic group of Indian emmer wheats. A set of 48 emmer wheat accessions from India including 28 from a local collection and 20 Indian accessions obtained from CIMMYT, Mexico, was assessed for genetic variability using 47 microsatellite (SSR) markers, distributed over all the 14 chromosomes. The number of alleles per locus ranged from 2 to 9, with an average of 3.87 alleles per locus. A total of 201 alleles were detected at 52 loci with average polymorphic information content of 0.35 per locus and a mean resolving power of 1. The pair-wise similarity coefficients calculated from binary data matrix based on presence or absence of alleles varied from 0.15 to 0.98, but was greater than 0.5 for most accessions, indicating a high level of similarity. A cluster analysis based on the similarity matrix identified nine distinct accessions and three clusters. All the recently developed commercial varieties were distinctly different from the clusters. Based on the analysis, it appears that Indian emmer wheats are not very diverse. Consequently, there is a need to increase the diversity within the Indian emmer wheat eco-geographic group, by introducing diversity from other eco-geographic groups, or even from other wheat species. 相似文献
12.
13.
Mohammad Moghaddam Bahman Ehdaie J. Giles Waines 《Genetic Resources and Crop Evolution》2000,47(3):323-334
Genetic variation and its distribution within and among 23 populations of Triticum urartu collected from Syria, Lebanon, Turkey, Armenia, and Iran was estimated using isozyme markers at eight polymorphic loci. The number of alleles per locus (A= 1.21), percentage polymorphic loci (P= 20.1%), and mean gene diversity (He= 0.024) were relatively low. In a population from Lebanon, a high number of alleles per locus (A= 2.13) and percentage polymorphic loci (P= 87.5%) was found. On average, genetic variation among populations (GST= 0.407) was smaller than within-population variation (0.593). However, different patterns of genetic structure were found among various geographic regions. Interpopulation variation was highest for the Iranian populations (0.89) followed by the Turkish populations (0.66). A reverse pattern was observed for the Syrian (0.11) and for the Lebanese (0.13) populations. The Armenian populations exhibited similar interpopulation and within-population variation. Principal component and cluster analyses resulted in distinct grouping of the geographically proximal populations, with the exception of the two Iranian populations. The Turkish populations were different from the neighboring Armenian populations compared to other countries. The populations from southern Syria and those from Lebanon also exhibited a high degree of genetic diversity. The two most heterozygous loci, Mdh-2 and Pgi-2, separated the populations along the first and second principal components, respectively. Most of the rare alleles were scattered sporadically throughout the geographic regions. Rare alleles with high frequencies were found in the Turkish and Armenian populations. These results indicated that different geographic regions require specific sampling procedures in order to capture the range of genetic variation observed in T. urartu populations. 相似文献
14.
M. R. Naghavi M. J. Aghaei A. R. Taleei M. Omidi J. Mozafari M. E. Hassani 《Genetic Resources and Crop Evolution》2009,56(4):499-506
Simple sequence repeats (SSRs), highly dispersed nucleotide sequences in genomes, were used for germplasm analysis and estimation
of the genetic relationship of the D-genome among 52 accessions of T. aestivum (AABBDD), Ae. tauschii (DtDt), Ae. cylindrica (CCDcDc) and Ae. crassa (MMDcr1Dcr1), collected from 13 different sites in Iran. A set of 21 microsatellite primers, from various locations on the seven D-genome
chromosomes, revealed a high level of polymorphism. A total of 273 alleles were detected across all four species and the number
of alleles per each microsatellite marker varied from 3 to 27. The highest genetic diversity occurred in Ae. tauschii followed by Ae. crassa, and the genetic distance was the smallest between Ae. tauschii and Ae. cylindrica. Data obtained in this study supports the view that genetic variability in the D-genome of hexaploid wheat is less than in
Ae. tauschii. The highest number of unique alleles was observed within Ae. crassa accessions, indicating this species as a great potential source of novel genes for bread wheat improvement. Knowledge of
genetic diversity in Aegilops species provides different levels of information which is important in the management of germplasm resources. 相似文献
15.
Milene Silvestrini Michele G. Junqueira Andréa C. Favarin Oliveiro Guerreiro-Filho Mirian P. Maluf Maria B. Silvarolla Carlos A. Colombo 《Genetic Resources and Crop Evolution》2007,54(6):1367-1379
Genetic diversity among 115 coffee accessions from the Coffea Germplasm Collection of IAC was assessed using SSR markers. The germplasm represents 73 accessions of Coffea arabica derived from spontaneous and subspontaneous plants in Ethiopia and Eritrea, species center of origin and diversity, 13 commercial
cultivars of C. arabica developed by the Breeding Program of IAC, 1 accession of C. arabica cv. ‘Geisha’, 13 accessions of C. arabica from Yemen, 5 accessions of C. eugenioides, 4 accessions of C. racemosa and 6 accessions of C. canephora. Genetic analysis was performed using average number of alleles per locus (A), proportion of polymorphic loci (P), Shannon’s genetic index (H′ and G′ST) and clustering analysis. All evaluated species were distinguished by a cluster analysis based on Jaccard’s coefficient.
Differentiation between the cultivated plants of C. arabica and accessions derived from spontaneous and subspontaneous plants was observed. Spontaneous and subspontaneous accessions
from Ethiopia were separated according to the geographical origin: east and west of the Great Rift Valley. Cultivated plants
showed a low genetic diversity with a division in two groups: accessions from Yemen (H′=0,028) and Brazilian commercial cultivars (H′=0,030). The results agreed with previously reported narrow genetic basis of cultivated plants of C. arabica and supported the hypotheses about domestication of the species. This study also showed a significant genetic diversity among
accessions from Ethiopia and Eritrea present in the Germplasm Collection of IAC. This diversity is specially observed in accessions
from Sidamo (H′=0,143), Kaffa (H′=0,142) and Illubabor (H′=0,147) indicating their importance as source of genetic variability for coffee breeding programs. 相似文献
16.
Alejandro Copete Roberto Moreno Adoración Cabrera 《Genetic Resources and Crop Evolution》2018,65(5):1455-1469
The genetic diversity was studied of 115 Agropyron cristatum accessions from 17 countries. Tetraploids were the most common (74.8%), followed by diploid (16.3%) and hexaploid (6.9%). We observed a relation between geographic distribution and ploidy level. The tetraploids, the most widespread, were found from Europe through Russia to East Asia. The diploids appeared over the same general range, except in Turkey, Iran and Georgia where no diploid accessions were found. Hexaploid accessions mainly came from a region comprising the east of Turkey, the north of Iran and Georgia. A selection of 71 accessions, including all three ploidy levels, were analyzed by capillary electrophoresis using six wheat simple sequence repeat (SSR) markers. All markers presented high levels of polymorphism, generating 166 different alleles ranging in size between 84 and 256 bp. Based on polymorphic information content values obtained (0.579–0.968), all the SSRs were classified as informative markers (values?>?0.5). According to the dendrogram generated, all the A. cristatum accessions were distinctly classified. Diploid, tetraploid and hexaploid accessions are not clearly differentiated from each other on the basis of SSR markers. A field experiment was conducted to morphologically characterize 18 accessions including the three ploidy levels. Significant differences were found between the accessions in spike length, spike width and number of spikelets per spike. All the cytological, molecular, and morphological data demonstrate the high genetic diversity present in A. cristatum, making it a valuable resource for future breeding programs. 相似文献
17.
J.B. Alvarez L. Caballero P. Ureña M. Vacas L.M. Martín 《Genetic Resources and Crop Evolution》2007,54(2):241-248
Emmer wheat is hulled wheat that was wide cultivated in Spain at the past. Actually, the most of this germplasm is conserved
in Germplasm Banks, and only two small populations have been found in Asturias (North of Spain) in a recent collecting mission.
In this work, a collection of 31 Spanish emmer lines developed from identical number of accessions of two Germplasm Banks
was analysed for morphological spike traits and seed storage protein composition. Up to seven different botanical varieties
were detected, which suggest the presence of a wide diversity, although lower than the historically described 10 botanical
varieties. At level of seed storage proteins, the lines showed a high diversity, although the new alleles were present with
low frequency in materials with scarce agronomic interest for the farmers (var. atratum, var. lagascae or var. pycnurum). This last circumstance could translate in a lost of variability by genetic drift. 相似文献
18.
Tufan Gökirmak Shawn A. Mehlenbacher Nahla V. Bassil 《Genetic Resources and Crop Evolution》2009,56(2):147-172
World hazelnut production is based primarily on selections from the wild. In this study, we used 21 pairs of simple sequence
repeat (SSR) primers to investigate genetic diversity in 270 clonal accessions of European hazelnut (Corylus avellana) representing a wide geographic range. Of the 270 accessions, 198 had unique fingerprints while 72 were duplicates. Based
on the 198 unique accessions, the number of detected alleles per locus averaged 9.81 and observed heterozygosity (Ho) averaged 0.67. Of the 206 total alleles amplified, 20 were unique to a single accession. A genetic similarity matrix was
constructed and the resulting dendrogram revealed four major geographical groups: Central European, Black Sea, English and
Spanish-Italian. SSR alleles indicated the parentage of 31 accessions. The fingerprints are publicly available through the
Germplasm Resources Information Network (GRIN) database. The identification of duplicate and mislabeled accessions will improve
management of hazelnut genebanks, and information on genetic variation in hazelnut will assist the international research
community.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
19.
Sevda Babayeva Zeynal Akparov Mehraj Abbasov Alamdar Mammadov Mohammad Zaifizadeh Kenneth Street 《Genetic Resources and Crop Evolution》2009,56(3):293-298
Diversity analysis was performed among 39 cultivated lentil (Lens
culinaris Medik.) accessions of Central Asia and Caucasian origin using five highly polymorphic microsatellite markers. A total of
33 alleles determined ranging from 3 to 8 per locus. Estimated gene diversity value for 33 loci was 0.66. Genetic similarity
indices among 39 accessions ranged from 0.24 to 1.0. Cluster analysis using the unweighted pair group method with arithmetic
mean method classified accessions into six major groups at 0.5 similarity coefficient. More than half accessions from Tajikistan
formed large cluster. On the other hand, a few accessions from each country showed unique genotypes. Overall, most of the
accessions, except ones with closely related origin, were distinguished by the present high quality DNA fingerprinting. This
molecular diversity information gives important basis for conservation strategy in gene bank and exotic germplasm introduction
in breeding programs in Central Asia and Caucasian countries. 相似文献
20.
The genetic diversity and similarities among 32 Kengyilia accessions, distributed to 14 species and one variety were analyzed by using random amplified microsatellite polymorphism
(RAMP) markers. Of the 160 RAMP primer combinations tested, 40 (25%) produced polymorphic and clear bands. A total of 264
bands were produced by 40 primer combinations, among which 231 out of 264 bands (87.5%) were polymorphic. Two to 11 polymorphic
bands could be amplified from each primer combination, with an average of 5.8 bands. The data of 264 bands were used for RAMP
assay. By NTSYS-pc program, genetic similarity coefficients were generated and dendrogram was constructed using UPGMA. The
genetic similarity coefficients ranged from 0.477 to 0.965 with the mean of 0.714. The results showed as follows: (1) distinct
genetic differences were present among the different species; (2) the different accessions in a species were clustered together,
respectively, which had larger genetic similarities and closer relations; (3) the species with similar morphological characters
and the species from the same areas or neighboring geographical regions were clustered together; (4) the lowest genetic similarity
was found between K. hirsuta (PI531618) and K. laxiflora (PI531631), while the highest genetic similarity was observed between K. hirsuta (Y2364) and K. hirsuta (Y2368); (5) RAMP results are basically comparable with those obtained from studies on morphology and cytology. It is a useful
method for analysis of the genetic diversity and similarities in Kengyilia. 相似文献