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在非疫区用H5亚型禽流感疫苗对20日龄以上鸡、鸭进行免疫试验,并对其结果进行比较和分析,得出不同日龄的鸡鸭免疫接种H5亚型禽流感疫苗20 d后其HI抗体效价不同,不同种类的家禽的免疫效果也不相同。75日龄肉鸡所产生的HI抗体效价最高,达6.4 log2。而75日龄肉鸭的HI抗体只有2.5 log2。 相似文献
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在非疫区用H5亚型禽流感疫苗对20日龄以上鸡、鸭进行免疲试验,并对其结果进行比较和分析,得出不同日龄的鸡鸭免疫接种H5亚型禽流感疫苗20d后其HI抗体效价不同.不同种类的家禽的免疫效果也不相同。75日龄肉鸡所产生的HI抗体效价最高,达6.4log2。而75日龄肉鸭的HI抗体只有2.5log2。 相似文献
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为了解野生水禽对高致病性禽流感病毒(AIV)自然被动免疫状况,于2010—2011年,在大庆龙凤湿地分别采集绿头鸭、须浮鸥、黑翅长脚鹬三种野生水禽巢卵150、184和128枚,采用血凝抑制试验检测H5和H7两种AIV的卵黄抗体。结果表明,绿头鸭H5和H7亚型禽流感卵黄抗体的阳性率分别为53.33%和14.67%,平均卵黄抗体滴度分别为4.55和3.45(log2);须浮鸥H5和H7亚型禽流感卵黄抗体的阳性率分别为34.78%和11.96%,平均卵黄抗体滴度分别为3.78和3.18(log2);黑翅长脚鹬H5和H7亚型禽流感卵黄抗体的阳性率分别为31.25%和9.38%,平均卵黄抗体滴度分别为3.85和3.67(log2)。由此可见,野生水禽种群对于高致病性禽流感的接触情况是较为频繁的,提示需要进一步加强对其种群的病毒携带情况进行监测。 相似文献
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Sanmartín ML Paramá A Castro R Cabaleiro S Leiro J Lamas J Barja JL 《Journal of fish diseases》2008,31(2):135-140
The efficacy of a vaccine against the fish pathogen Philasterides dicentrarchi was evaluated in turbot by measuring the production of specific antibodies and duration of protection. Four groups of turbot were vaccinated twice, on days 0 and 30, with phosphate-buffered saline, mineral oil adjuvant, antigen or antigen plus adjuvant. Specific serum antibodies were determined on day 0 and 1 month after the first and the second vaccinations. Protection was evaluated 1 month after the first vaccination and 1 and 5 months after the second vaccination. Serum antibody titres, measured by enzyme-linked immunosorbent assay, and protection, assessed by challenges, increased significantly 1 month after the second vaccination in the group injected with antigen plus adjuvant and the protection lasted for at least a further 5 months in this group. The relative protection was 77% and 66% 1 and 5 months after the second vaccination, respectively. Administration of antigen or adjuvant separately had no effect on antibody response or protection. The results indicate that emulsion containing antigen plus adjuvant induced durable protection against P. dicentrarchi after the administration of the two vaccinations, and that this preparation can be used as a vaccine against the pathogen. 相似文献
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在骡鸭与樱桃谷鸭的填肥料中添加4%玉米油或鸭油,研究添加动植物油脂填饲对鸭产肝性能的差异影响。结果表明:添加玉米油,与添加鸭油填饲相比,2个品种鸭获得较高的肝重与较低的料肝比,且骡鸭添加玉米油组的肥肝率显著高于鸭油组(高出30%);经填饲,骡鸭的肥肝重较樱桃谷鸭高,同时料肝比较低,但差异均不显著(P>0.05)。意味着肥肝生产中,填饲时添加玉米油较添加鸭油更利于提高产肝性能;并且对骡鸭更有效。对于其根本原因,需进一步从分子水平上研究鸭肝的生脂酶基因、与甘油三酯分泌及脂肪分解相关酶基因的表达差异。 相似文献
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鱼油微囊化工艺及其特性研究 总被引:1,自引:0,他引:1
研究了鱼油微囊工艺及特性,采用喷雾干燥法进行鱼油微囊化研究。混合表面活性剂最适HLB值为7.5。乳液粘度影响乳液稳定性及喷雾干燥效果。固形物含量与乳液粘度有关。筛选出最适壁材是阿拉伯胶、鼓甲基纤维素、蛋白粉。微囊鱼油具有良好的稳定性。微囊工艺改善了鱼油的风味。 相似文献
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Duck plague: clinical aspects, diagnosis, control 总被引:1,自引:0,他引:1
M Weingarten 《Tier?rztliche Praxis》1989,17(1):53-56
A predominantly acute, highly contagious disease of the waterfowl is called duck plague or virus enteritis of ducks. The pathogenic agent is a herpes virus, type A. The clinical course of the disease and its pathological-anatomical findings are described and the possibility of prophylactic immunization is discussed. The results of lab trials demonstrate the effectiveness of a vaccine against the duck plague. 相似文献
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Comparison of different challenge methods to evaluate the efficacy of furunculosis vaccines in Atlantic salmon, Salmo salar L. 总被引:1,自引:0,他引:1
Four challenge methods, intraperitoneal (i.p.) and intramuscular (i.m.) inoculation, bath and cohabitation exposure, were evaluated as methods for testing the efficacy of furunculosis vaccines in Atlantic salmon, Salmo salar L. Groups of fish vaccinated with one of two different vaccines containing aluminium phosphate or animal/vegetable oil as an adjuvant were challenged with Aeromonas salmonicida 6 and 12 weeks after vaccination. Relative per cent survival (RPS) was calculated daily during a 3-week observation period post-challenge. A large variation in protection measured by RPS, both between methods and between different time points for each method, was found. Towards the end of the observation period, RPS tended to be similar in the i.p. and cohabitation challenge groups. A high degree of protection was demonstrated for the oil adjuvanted vaccine. The i.m. challenge produced very low RPS numbers for both vaccines, but this was most marked for the aluminium-phosphate-adjuvanted vaccine. The bath exposure resulted in RPS values intermediate to the cohabitation and injection methods. The study also demonstrated that, after i.p. and i.m. challenges, the initial peak mortality caused by the inoculation was followed by a secondary increase in mortality, probably because of shedding of bacteria into the water during the first mortality phase and hence contributing to a superinfection state. 相似文献
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应用重组禽流感病毒灭活疫苗(H5N1亚型,Re-1株),禽流感-新城疫重组二联活疫苗(rL-H5株),首免用重组禽流感病毒灭活疫苗(H5N1亚型,Re-1株)、二免用禽流感-新城疫重组二联活疫苗(rL-H5株)对3组肉鸡进行禽流感的免疫效果观察。结果表明:免疫重组禽流感病毒灭活疫苗(H5N1亚型,Re-1株)的效果比免疫禽流感-新城疫重组二联活疫苗(rL-H5株)的效果好;首免用重组禽流感病毒灭活疫苗(H5N1亚型,Re-1株)、二免用禽流感-新城疫重组二联活疫苗(rL-H5株)的效果比免疫重组禽流感病毒灭活疫苗(H5N1亚型,Re-1株)的效果更好。 相似文献
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James M Barron Ronald G Twibell Heidi A Hill Kyle C Hanson Ann L Gannam 《Aquaculture Research》2016,47(12):3899-3906
The Pacific lamprey Entosphenus tridentatus is an ancestral species of critical importance to the ecosystem and indigenous cultures in the Pacific Northwest. Conservation aquaculture has been proposed as a potential technique to restore Pacific lamprey populations. Intensive culture methods and diets for this species have not been developed. A sixteen week feeding trial tested the effects of seven diet treatments on the survival, growth, fatty acid profile and whole body lipid content of Pacific lamprey ammocoetes. Dietary treatments were: active dry yeast, yeast plus fish oil emulsion, micro‐algae, micro‐algae plus fish oil emulsion, yeast with micro‐algae, yeast with micro‐algae plus fish oil emulsion and yeast with larval fish diet. Each diet was offered to five replicate tanks stocked with 20 ammocoetes that were 51 days post hatch. Survival during the trial was not affected by diet. The greatest length and weight increases were in fish fed diets containing yeast. Growth decreased as the amount of algae in the diet was increased. Lipid retention was significantly higher in fish fed yeast with larval fish diet relative to the other treatments. Feed conversion ratio was lowest in fish fed diets containing yeast. Whole body fatty acid profiles tended to reflect the fatty acid profile of the diet. Percentages of 20:5n‐3 and 22:6n‐3 were significantly higher in fish fed diets containing fish oil emulsion. Overall, yeast with larval fish diet provided the best growth performance in larval Pacific lamprey. 相似文献
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A new water-in-oil-in-water (W/O/W) type emulsion using lipophilized gelatin (LG) and cotton-seed oil was developed for a novel method of hormone administration in fishes. LG was prepared by attaching palmitic anhydride to gelatin. The best conditions for preparing stable emulsions were determined where the final concentrations of LG in the emulsion and the volume ratio of LG solution to cotton-seed oil were 2% and 1:2, respectively. Hormone releasing properties of LG emulsion were compared with a water-in-oil (W/O) type emulsion prepared with Freund's incomplete adjuvant (FIA) and saline solution by monitoring plasma profiles of salmon gonadotropin (sGtH) II in the Japanese eel. sGtH II is gradually released from the LG emulsion. LG emulsion had hormone-releasing properties different from those of the saline solution and the FIA emulsion. Immature female Japanese eel (BW, 566 to 1017 g) received a weekly intramuscular injections (total of 10 injections) of the LG emulsion, the FIA emulsion or the saline solution, each of which contained sGtH fraction. The LG emulsion was found to be more effective in inducing ovarian maturation. In mature eel, final maturation and ovulation could be induced by simultaneous administration of sGtH fraction and 17-hydroxyprogesterone using LG W/O/W emulsion. 相似文献