影响DNA指纹技术进行玉米亲子鉴定准确性的主要因素研究

利用DNA指纹技术,分别采用不同数量的SSR引物,对14个同母异父杂交组合及其父本进行亲子鉴定,研究影响玉米亲子鉴定结果准确性的主要因素,结果表明:(1)与母本具有共同血缘的自交系作为父本将影响亲子鉴定的准确性;(2)采用引物数量不同,检测的DNA片段数量不同,也影响鉴定结果的准确性,采用引物数量越多,检测的DNA片段数量越多,鉴定结果越准确.     

利用DNA指纹技术进行玉米亲子鉴定方法研究

利用DNA指纹分析技术 ,分析了同母异父 5个杂交组合分别与其父母本之间的遗传距离 ,结果发现 ,每个杂交组合与其相应的父本之间的遗传距离最小 ,所有组合完全一一对应。因此 ,可以通过计算遗传距离来进行玉米亲子鉴定 ,只需计算出玉米杂交种与所有可能父本自交系之间的遗传距离 ,与其遗传距离最小的自交系就是该杂交种的真正父本。     

A Key Technology in Automatic Fingerprint Identification System:Directional Image

Because fingerprint directional image represents the basic structural feature really and abstractly, It has very important research value in automatic fingerprint identification system. Now it has been widely used to solve the problem of image enhancement, global feature extraction, automatic classification, template matching and image encoding. Especially the Gabor filter based on directional image gets a marvelous effect, which can filter the noises along ridges. Combining the author's research work, several newly developed methods to get directional image are introduced. The performance of real-time and robust to noise of these methods are assessed,and the mending methods are presented also. In the end, the typical applications are systematically overviewed and the direction for further study is provided.     

Fingerprint Identification Using Point Pattern Matching

Fingerprint is those lines distributing on the finger surface. It's unique and stable. It has been hundreds years to use fingerprint to identity. AFIS (Auto Fingerprint Identification System) has been widely used. Besides traditional criminal, AFIS has been used to validate user in OS and web access, and embedded AFIS also has been used in ATM, credit card and door-lock. Fingerprint matching is one of the most important problems in AFIS. In general, the minutiae such as ridge endings and ridge bifurcation is to be used to represent a fingerprint and the fingerprint matching through minutiae matching. Based on this observation and by representing the minutiae as a point pattern, an automatic fingerprint verification problem may be reduced to a point pattern matching (minutia matching) problem. Point pattern matching is a famous problem in the field of pattern recognition. For a good point pattern matching approach, it is able to perform the geometrical invariant quantities (translation, rotation, and scaling) efficiently. Various algorithms have been proposed for point pattern matching. For example, the relaxation approach handles translational difference only and the complexity of triangles approach is very high. In this paper, an algorithm for fingerprint identification using point pattern matching based on cluster approach is proposed, which effectively solves the problems of optimal matching between two fingerprint minutiae images under geometrical transformation and minutiae quantity change. Process which bases on the matching of vector pairs is developed to determine the registration parameters. The experimental results show that the proposed matching algorithm is fast and has high accuracy.     

新疆早熟棉品种SSR指纹图谱构建与品种鉴别

以新疆自1978年以来审定命名的42个早熟棉品种为材料,利用SSR分子标记对新疆早熟棉品种进行研究。从2300对SSR引物中筛选出了52对具有稳定多态性的引物,每对引物可检测到3～24个多态性片段,共检测到506个,平均9.7个,片段大小介于100～2000bp之间。对所得到的52个SSR指纹图谱进行组合和综合分析,用其中2对就可将所有供试品种区分开来。同时,分别对42个早熟棉品种进行指纹分析,获得各个品种的特异性指纹,可为今后棉花种子真伪快速鉴别奠定基础。     

Mouse with Capablility of Fingerprint Identifying

The mouse is developed with the purpose of providing reinforcement of database security for computer users, whose validity can be verified by taking advantage of the fingerprint identity, which is the uniqueness, immutability biological characteristic of human body. By one-by-one matching algorithm, rendering between pressured data and field acquisitions of finger print image, the vital heart of dactylogram verification with the mouse is realized. The mouse has been made possible by taking account of the roomy space within mouse,tiny chip patch for fingerprint identification and together with small size of available microprocessors. The mouse can be used as an normal pointing device while maintaining the capability of user identity distinguishing. The usage of this device is very easy and it is reliable. Development of this device mainly comprises of the hardware development, driver development, application software development, and the installation of network security verification center. The purpose, principle and system design of the mouse is introduced, while characteristics and applications of the device are also presented.     

阿魏蘑的DNA指纹分析与鉴定

为及时制止阿魏蘑菌株混乱现象的产生,对国内现有的11株栽培常用的阿魏蘑菌株进行DNA指纹分析;以RAPD、ISSR、SRAP多态性分析的方法为主,用筛选出来的8条RAPD、ISSR、SRAP引物共扩增出116个较为清晰的DNA条带,然后进行聚类分析;结果表明,可将11个供试菌株分为四个群体：类Ⅰ：包括Pl. f 0007;类Ⅱ：包括Pl. f 0002;类Ⅲ：包括Pl. f 0003;类Ⅳ：包括余下的8个菌株。本研究为阿魏蘑的核心种质的构建和资源的科学保存提供了依据,并为规范阿魏蘑的生产奠定了基础。     

Improved Cosine Window and Its Implementation with DSP

An improved cosine window is put forward, which can reduce the spectrum leakage and can be easily implemented. The center of the method is to apply an exponent-m-to the usual window function. A proper value can be chosen for the exponent in light of the particular emphasis on frequency resolution, amplitude precision or sidelobe suppression and we can modify the characteristic of the window function through altering the value of m. Cycle of different times is effect.Carried out to implement it in DSP with software. The result of the emluator makes it clear that it is a method with good effect.     

依赖性派生玉米品种DNA指纹鉴定标准研究

模仿育种是一快速、高效、针对性强的(改良)育种方法,但是根据最新国际保护公约规定,所育品种属于依赖性派生品种,侵害了原品种权人的权利。改良品种与原品种的遗传差异多大才算是依赖性派生品种,目前国内外没有标准。以BA为母本,以H28、昌7-29、502、P138、齐319、连87、吉853、京2101、京343、京832为父本组配了10对同母异父玉米杂交组合,研究了杂交组合两两之间的遗传差异。结果表明:遗传差异在20%以下的组合,父本之间具有明确的血缘关系;遗传差异在20%~30%的组合,父本之间有40%具有血缘关系,没有血缘关系的占60%;遗传差异在30%以上的组合,父本之间全部没有血缘关系。无论两父本之间是姊妹系,还是其中一个父本是用另一个父本回交2次改良而成,用它们组配的杂交组合之间的遗传差异都在10%以上。通过回交3次改良的组合,与原组合之间的遗传差异在10%以下。因此,将遗传差异小于10%作为判断是否是依赖性派生品种的DNA指纹鉴定标准。     

Investigation on ARM9-based Embedded Fingerprint Identification System

This paper introduces an implementation method of Embedded Automatic Fingerprint Identification Systerm(AFIS), dealing mainly with hardware components and software modules based on ARM9. Hardware system consists of ARM9 series S3C2500, FPS200 sensor, memories, power supply ,communication interface, etc. System initiation, fingerprint sampling procedure and communication program are main modules making up of system software.The conflict of time-sequence between S3C2500 and sensor FPS200-one of the key links of the system, has been solved successfully. The system has integrated functionalities,such as fast sampling, communicating with upper and lower computers, establishing a complete and reliable platform for fingerprint image processing and identification.     

基于SSR分子标记的8个薏苡新材料鉴定与品种比较试验

为筛选适宜贵州省栽培种植的优良品种,以Y10、ZY、Y42、QL、Y43、Y8、Y160、ZF等8个薏苡新材料为试验材料,以‘兴仁小白壳’为对照,采用SSR标记对8个材料间的亲缘关系进行了鉴定,随机区组设计进行品种比较试验。结果表明:将20对组合的SSR引物扩增结果进行聚类,遗传相似系数变化范围为0.467～0.922,供试材料间存在差异,说明这9个参试材料间各不相同;2015—2016年品种比较试验结果表明,Y10、ZY、Y42、QL、Y160与对照的产量比较均达到极显著水平,Y10 2年产量均最高,说明筛选出的Y10、ZY、Y42、QL、Y160薏苡新材料具有显著的优势,为薏苡新品种选育提供参考。     

ADSL Self-adapting detecting Method Based on DSP

Asymmetrical Digital Subscriber Loop (ADSL) is suitable to eliminate "the last mile" bottleneck of current broadband solutions, which use the existing telephone network and apply more and more abroad. It results in the higher demands of the detecting equipment. According to the review and comparison of today "s ADSL detect instruments, a new method is put forward with the system design, which self - adapting detecting can be used to test the ADSLs transmission characters on the whole band dynamically. Current DSP is used to operate arithmetic. The self - adapting detecting arithmetic can get the characteristic of signal channel through the MATLAB simulation.     

Accurately Extraction of Fingerprint Ridge''s Local Average Period

The period of local ridges is a critical parameter to constitute filters (for instance Gabor Filter) used in pre-processing of the fingerprint images. Behind acquiring orientation of local ridges, this thesis analyzes the influence of the wrong period of local ridges upon filtering , and presents a method for accurately extracting the period of local ridges on the basis of Mathematical Morphology . Numerous experimental results show that the method is effective and reliable.     

基于HPLC指纹图谱技术的烤烟质量分类研究

为解决当前烤烟质量分类费时费力且主观偶然性强的问题,采用高效液相指纹图谱技术,对来自多个省份的典型烟叶产区共14批B2F下限等级烟叶进行图谱信息采集,并基于14批烟叶图谱进行主成分提取、相关性分析和聚类分析。选取前5个主成分进行分析。相关性分析表明,钾和主成分值之间呈极显著相关。聚类分析表明,来自多个省份典型烟叶产区的烟叶可大致分为3类。其中第1类样品与第2类样品的钾含量差异明显;第2类样品中氯的含量明显高于第1、3类样品。试验结果表明,烟叶的HPLC指纹图谱可以作为烟叶质量控制的新方法,此方法可以提高烟草品质判别效率,降低人工评价的个体差异。     

Detecting Technology of Cabel Surface Defect Based on DSP and Image Recognition

The damage of bridge cable directly hazards the normal use of bridge and the safety of people. The paper discussed the signal acquisition and detection system which takes embedded DSP processor as core technology, combined with CCD, video decode chip, SDRAM, FLASH ROM, and established the fast measuring algorithm of the surface corrosion and damage, which includes deteminating the processing region, improving medium filter, sobel edge detection and dynamic threshold segment, close operation of morphology, computing defect area and saving. The experimental resuits show that this method implemented the real-time, non-contact and intelligence detecting of cable surface defect and gives the specifications of the system.     

Virtual FFT Spectrum Analyzer Based on DSP Technology

Virtual Instrument has become an important direction of instrument development, now it has been widely used in many fields. FFT analysis is a widely used method in mechanical engineering, fault detection and many other fields. However, the traditional FFT analyzer is expensive and is not easy to upgraded. The birth of virtual FFT Spectrum Analyzer gets rid of the limit of traditional FFT analyzer, and paves the way for the application of FFT analyzer. The uses of DSP technology make the future broadened of Virtual Instrument. This article ,based on the deep study of DSP system, designs a virtual FFT Spectrum analyzer based on DSP technology. The design is new and of great practicality, which indicates the important role of Virtual Instrument in the development of instruments.     

‘湘农大棉1号’的SSR数字指纹图谱构建及纯度鉴定

为了快速而准确地鉴定杂交棉种子的纯度和真伪,根据亲本材料来源和被推荐使用的SSR核心引物,挑选了33对引物对‘湘农大棉1号’及其亲本进行多态性筛选。结果表明,其中8对SSR引物在父母本及其杂交种扩增出清晰、稳定的多态性性条带,且这些位点在F1中均表现为共显性标记。获得的共显性标记都能用于该杂交种的纯度鉴定,可以用来区别其中混杂的母本、父本及其他种子。将这8对引物在3个材料中扩增得到的01（二进制）数据转换成十进制数据,构建了‘湘农大棉1号’及其亲本的数字指纹图谱。采用多对引物构建的杂交棉数字指纹图谱,能为杂交种的真伪鉴定、纯度检测及亲本提纯等工作提供更加准确的技术指导。     

Development of Data Acquisition and Processing System Based on DSP and USB 2.0

The data acquisition and processing system are proceeding towards faster, more powerful, easier to use and more cost effectiveness. This paper discuss the design of the high speed and convenient data acquisition and processing system based on DSP and USB 2.0, and expatiates the principle and method of the virtual instruments system in detail. The system employs an ADS8346 to sample the six channel signals simultaneously with a resolution of 16-bit.It utilizes EZ-USB FX2 as the USB2.0 interface, which can achieve the high speed communication between the host and the system. It uses TMS320VC33 (dsp) as the core chip, which can accomplish the function of the main control and real-time digital signal processing. The system enhances the virtual instruments' advantage more by the combination between software and hardware.     

用Rim2超级家族分子指纹鉴别杂交水稻及预测杂种优势

选用5对Rim2引物对21份籼稻和20份粳稻材料进行了DNA指纹扩增,共扩增出38条谱带,其中多态性谱带26条,多态性水平为69.64%;粳稻平均多态性水平为56.67%,明显高于籼稻。根据Nei’s遗传距离计算获得的聚类树状图表明,参试21份籼稻材料聚为6组,20份粳稻材料聚为7组。亲缘关系分析表明,Rim2分子指纹可以区分多数的籼稻或粳稻材料,且显示出材料的遗传特征。考察部分籼稻和粳稻杂交组合的产量对照优势与双亲间遗传距离的关系,发现杂交粳稻的遗传距离与杂交优势表现出相关性,而杂交籼稻无此相关性。     

ISSR分子标记技术在桃品种鉴定中的应用

为了有效地鉴别桃苗木优良品种或类型,为桃种质资源研究和新品种保护提供理论基础,利用ISSR标记对28份桃种质进行了品种鉴定及亲缘关系检测。结果发现：23条ISSR引物共扩增出188条DNA片段,其中96条具有多态性,多态性频率为51.06%;材料间遗传相似系数GS变幅为0.84～0.97,平均为0.92。获得28份供试材料的指纹图谱,鉴定出9条特异出现DNA片段和11条特异缺失DNA片段。通过UPGMA聚类分析发现,供试材料中地理来源及部分农艺性状相近的品种或类型聚为小类,但大的聚类群没有明确的划分标识,可能与桃种质在地区间互换及所选择的ISSR标记有关。